DE3030185A1 - Oxalate removal from e.g. foods, beverages or biological fluids - by reaction with oxygen in the presence of oxalate oxidase to give carbon di:oxide and water - Google Patents
Oxalate removal from e.g. foods, beverages or biological fluids - by reaction with oxygen in the presence of oxalate oxidase to give carbon di:oxide and waterInfo
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- DE3030185A1 DE3030185A1 DE19803030185 DE3030185A DE3030185A1 DE 3030185 A1 DE3030185 A1 DE 3030185A1 DE 19803030185 DE19803030185 DE 19803030185 DE 3030185 A DE3030185 A DE 3030185A DE 3030185 A1 DE3030185 A1 DE 3030185A1
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- oxalate
- oxygen
- oxidase
- beverages
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- MUBZPKHOEPUJKR-UHFFFAOYSA-N Oxalic acid Chemical compound OC(=O)C(O)=O MUBZPKHOEPUJKR-UHFFFAOYSA-N 0.000 title claims abstract description 55
- 108010063734 Oxalate oxidase Proteins 0.000 title claims abstract description 21
- 239000001301 oxygen Substances 0.000 title claims abstract description 9
- 229910052760 oxygen Inorganic materials 0.000 title claims abstract description 9
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 title claims abstract description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 title claims abstract description 6
- 239000013060 biological fluid Substances 0.000 title claims abstract 3
- 235000013305 food Nutrition 0.000 title claims description 7
- 235000013361 beverage Nutrition 0.000 title abstract description 5
- 238000006243 chemical reaction Methods 0.000 title description 5
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 title 1
- 229910052799 carbon Inorganic materials 0.000 title 1
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 claims abstract description 16
- 235000013405 beer Nutrition 0.000 claims abstract description 11
- 238000000034 method Methods 0.000 claims abstract description 11
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 claims abstract description 10
- 102000016938 Catalase Human genes 0.000 claims abstract description 10
- 108010053835 Catalase Proteins 0.000 claims abstract description 10
- 102000004190 Enzymes Human genes 0.000 claims abstract description 8
- 108090000790 Enzymes Proteins 0.000 claims abstract description 8
- 229910002092 carbon dioxide Inorganic materials 0.000 claims abstract description 5
- 239000001569 carbon dioxide Substances 0.000 claims abstract description 5
- 244000005700 microbiome Species 0.000 claims abstract 3
- 241001122767 Theaceae Species 0.000 claims abstract 2
- 235000013399 edible fruits Nutrition 0.000 claims abstract 2
- 230000002255 enzymatic effect Effects 0.000 claims abstract 2
- 235000011389 fruit/vegetable juice Nutrition 0.000 claims abstract 2
- 210000002966 serum Anatomy 0.000 claims abstract 2
- 235000013616 tea Nutrition 0.000 claims abstract 2
- 210000002700 urine Anatomy 0.000 claims abstract 2
- 238000009825 accumulation Methods 0.000 abstract description 2
- 238000006911 enzymatic reaction Methods 0.000 abstract description 2
- 239000000126 substance Substances 0.000 abstract description 2
- 231100000331 toxic Toxicity 0.000 abstract description 2
- 230000002588 toxic effect Effects 0.000 abstract description 2
- 238000004458 analytical method Methods 0.000 abstract 1
- 238000002845 discoloration Methods 0.000 abstract 1
- 239000000383 hazardous chemical Substances 0.000 abstract 1
- 231100000206 health hazard Toxicity 0.000 abstract 1
- 210000002381 plasma Anatomy 0.000 abstract 1
- 239000000243 solution Substances 0.000 description 7
- 230000000694 effects Effects 0.000 description 5
- 230000003197 catalytic effect Effects 0.000 description 4
- 150000003891 oxalate salts Chemical class 0.000 description 4
- 235000006408 oxalic acid Nutrition 0.000 description 3
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 2
- 102000003855 L-lactate dehydrogenase Human genes 0.000 description 2
- 108700023483 L-lactate dehydrogenases Proteins 0.000 description 2
- 108010053763 Pyruvate Carboxylase Proteins 0.000 description 2
- 102000013009 Pyruvate Kinase Human genes 0.000 description 2
- 108020005115 Pyruvate Kinase Proteins 0.000 description 2
- 102100039895 Pyruvate carboxylase, mitochondrial Human genes 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 229910052791 calcium Inorganic materials 0.000 description 2
- 239000011575 calcium Substances 0.000 description 2
- 239000003054 catalyst Substances 0.000 description 2
- 239000007795 chemical reaction product Substances 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 235000015203 fruit juice Nutrition 0.000 description 2
- 150000003839 salts Chemical class 0.000 description 2
- 238000003860 storage Methods 0.000 description 2
- 208000009043 Chemical Burns Diseases 0.000 description 1
- 240000005979 Hordeum vulgare Species 0.000 description 1
- 235000007340 Hordeum vulgare Nutrition 0.000 description 1
- 208000000913 Kidney Calculi Diseases 0.000 description 1
- 206010029148 Nephrolithiasis Diseases 0.000 description 1
- 239000004677 Nylon Substances 0.000 description 1
- 208000004880 Polyuria Diseases 0.000 description 1
- LCTONWCANYUPML-UHFFFAOYSA-M Pyruvate Chemical compound CC(=O)C([O-])=O LCTONWCANYUPML-UHFFFAOYSA-M 0.000 description 1
- 244000269722 Thea sinensis Species 0.000 description 1
- 208000009911 Urinary Calculi Diseases 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 230000031018 biological processes and functions Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 239000013078 crystal Substances 0.000 description 1
- 230000008021 deposition Effects 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 150000002085 enols Chemical class 0.000 description 1
- 229940125532 enzyme inhibitor Drugs 0.000 description 1
- 239000002532 enzyme inhibitor Substances 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 235000020006 fruit beer Nutrition 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 210000001035 gastrointestinal tract Anatomy 0.000 description 1
- GPRLSGONYQIRFK-UHFFFAOYSA-N hydron Chemical compound [H+] GPRLSGONYQIRFK-UHFFFAOYSA-N 0.000 description 1
- 230000001771 impaired effect Effects 0.000 description 1
- 230000002452 interceptive effect Effects 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 229910021645 metal ion Inorganic materials 0.000 description 1
- 229920001778 nylon Polymers 0.000 description 1
- 235000015205 orange juice Nutrition 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- -1 oxalate anion Chemical class 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 239000013049 sediment Substances 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 231100000167 toxic agent Toxicity 0.000 description 1
- 208000008281 urolithiasis Diseases 0.000 description 1
- 235000015192 vegetable juice Nutrition 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23F—COFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
- A23F3/00—Tea; Tea substitutes; Preparations thereof
- A23F3/16—Tea extraction; Tea extracts; Treating tea extract; Making instant tea
- A23F3/166—Addition of, or treatment with, enzymes or microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
- A23L5/20—Removal of unwanted matter, e.g. deodorisation or detoxification
- A23L5/25—Removal of unwanted matter, e.g. deodorisation or detoxification using enzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12H—PASTEURISATION, STERILISATION, PRESERVATION, PURIFICATION, CLARIFICATION OR AGEING OF ALCOHOLIC BEVERAGES; METHODS FOR ALTERING THE ALCOHOL CONTENT OF FERMENTED SOLUTIONS OR ALCOHOLIC BEVERAGES
- C12H1/00—Pasteurisation, sterilisation, preservation, purification, clarification, or ageing of alcoholic beverages
- C12H1/003—Pasteurisation, sterilisation, preservation, purification, clarification, or ageing of alcoholic beverages by a biochemical process
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Organic Chemistry (AREA)
- Polymers & Plastics (AREA)
- General Engineering & Computer Science (AREA)
- Physiology (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- General Chemical & Material Sciences (AREA)
- Biochemistry (AREA)
- Nutrition Science (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- General Preparation And Processing Of Foods (AREA)
Abstract
Description
Die Erfindung betrifft ein enzymatisches Verfahren zur Ent-The invention relates to an enzymatic method for developing
fernung von Oxalat.removal of oxalate.
Oxalsäure ist eine starke organische Säure. In Anwesenheit von Metallionen bilden sich schwerlösliche Salze, Oxalate, die wie die Säure vielfältige störende Einflüsse zeigen. A. HODGKINSON gibt in der Monographie Oxalat Acid in Biology and Medicine", Academic Preis, London 1977, 325 pp, ISBN 012-351750-8, eine Zusammenfassung. Auf einige schädliche und störende Einflüsse von Oxalat soll hier hingewiesen werden: in der Human- und Tiermedizin ist die Gefährlichkeit der Oxalsäure und ihrer Salze wohlbekannt. Diese Verbindungen können z.B. Zähne schädigen, Lebensmittel ansäuern, Kalzium aus der Nahrung und aus wichtigen biologischen Prozessen entziehen, im Verdauungstrakt Verätzungen hervorrufen sowie zu den gefürchteten Nieren- und Harnsteinen führen. Bei z.B. Fruchtsäften, bei diureseförderndem Bier und bei Tee würde eine Senkung des Oxalatgehaltes eine Steigerung des gesundheitlichen Wertes bedeuten. Getränke werden zudem durch die Bildung von Oxalatkristallen sowie durch Barbveränderung aufgrund einer Oxalsäure-verursachten pH-Senkung in ihrem optischen Aussehen beeinträchtigt.Oxalic acid is a strong organic acid. In the presence of metal ions Hardly soluble salts, oxalates, are formed which, like the acid, have a variety of disruptive effects Show influences. A. HODGKINSON gives in the monograph Oxalate Acid in Biology Medicine ", Academic Price, London 1977, 325 pp, ISBN 012-351750-8, an abstract. Some of the harmful and disruptive influences of oxalate should be pointed out here: in human and veterinary medicine is the danger of oxalic acid and its salts well known. These compounds can, for example, damage teeth, acidify foods, Withdraw calcium from food and important biological processes, in the digestive tract Can cause chemical burns and lead to the dreaded kidney and urinary stones. For example, fruit juices, diuresis-promoting beer and tea would decrease the oxalate content mean an increase in the health value. beverages are also caused by the formation of oxalate crystals and changes in Barb their visual appearance is impaired due to a decrease in pH caused by oxalic acid.
Aus dem Braugewerbe sind seit langem vielfältige Bemühungen bekann, den Oxalatgehalt des Bieres zu vermindern. Oxalate bewirken in Bieren einen unerwünschten silbrigen Glanz, und es kann zur Ablagerung dieser Oxalate in Form von kristallinen Bodensätzen kommen, Es sind Versuche bekannt, durch Verwendung von Braugerste mit möglichst geringem Oxalatgehalt ein möglichst oxalatarmes Bier zu erhalten. Daneben wurde dem Brauwasser Kalzium zugesetzt, d.h. es wurde "aufgehärtet", um möglichst viel Oxalat schon in den ersten Herstellungsprozessen auszufällen.Various efforts have long been known from the brewing industry to reduce the oxalate content of the beer. Oxalates cause an undesirable effect in beers silvery sheen, and there can be deposition of these oxalates in the form of crystalline ones Sediments come, There are known attempts by using brewing barley with to obtain a beer that is as low in oxalate as possible with the lowest possible oxalate content. Besides Calcium was added to the brewing water, i.e. it was "hardened" in order to to precipitate a lot of oxalate in the first manufacturing processes.
Auch durch längere Lagerung bei tiefen Temperaturen sollte eine Ausfällung von Oxalaten und damit nach Filtrierung eine Verminderung des Oxalatgehaltes im Bier erreicht werden.Precipitation should also occur through prolonged storage at low temperatures of oxalates and thus, after filtration, a reduction in the oxalate content in the Beer can be achieved.
Alle diese Bemühungen lieferten nicht den gewünschten Erfolg.All of these efforts did not produce the desired results.
Kommerziell erhältliches Bier enthält derzeit ca. 10 - 30 mg Oxalat je Liter, es kommen aber auch Konzentrationen von 50 mg Oxalat je Liter und mehr vor. Bei einem zugrundegelegten Molekulargewicht von 9) für die wasserfreie Oxalsäure entspricht dies Konzentrationen von 111 - 333 pmol/l bzw. 555 pmol/l.Commercially available beer currently contains approx. 10-30 mg oxalate per liter, but there are also concentrations of 50 mg oxalate per liter and more before. With an underlying molecular weight of 9) for the anhydrous oxalic acid this corresponds to concentrations of 111 - 333 pmol / l or 555 pmol / l.
Allgemein werden aus den vorgenannten gesundheitlichen und lebensmitteltechnologischen Gründen Nahrungs- und Genußmittel mit einem möglichst niedrigen Oxalatgehalt angestrebt.In general, the aforementioned health and food technology The aim is to create foodstuffs and luxury foods with the lowest possible oxalate content.
Das Oxalat-Anion spielt auch eine schädliche und störende Rolle als Enzyminhibitor. Wegen seiner Strukturanalogie mit (Enol-) Pyruvat sind u.a. Lactat-Dehydrogenase (EC 1.1.1.27), Pyruvat-Kinase (EC 2.7.1.40) und Pyruvat-Carboxylase (EC 6.4.1.1) betroffen. Bei ensynatischen Aktivitäts- und gegebenenfalls auch bei Substratbestinmungen in der Klinischen Chemie verfälscht somit in der Probe vorhandenes Oxalat die Meßergebnisse. Dabei sind das Ausmaß der Störungen und damit die Genauigkeit der Bestimmungen meist sicht bekannt, da die interferierenden OxaJa-bmengen unbekannt bleiben.The oxalate anion also plays a harmful and disruptive role as Enzyme inhibitor. Because of its structural analogy with (enol) pyruvate, lactate dehydrogenase is among others (EC 1.1.1.27), pyruvate kinase (EC 2.7.1.40) and pyruvate carboxylase (EC 6.4.1.1) affected. With ensynatic activity and possibly also with substrate determinations In clinical chemistry, oxalate present in the sample thus falsifies the measurement results. The extent of the disturbances and thus the accuracy of the determinations are mostly known, since the interfering OxaJa-b quantities remain unknown.
Aufgabe der Erfindung ist es, ein einfaches Verfahren zur Entfernung von Oxalat zur Verfügung zu stellen.The object of the invention is to provide a simple method for removal of oxalate available.
Die Aufgabe wird erfindungsgemäß gelöst, indem Oxalat und Sauerstoff unter Verwendung des bisher nicht zur Oxalatentfernung beschriebenen Enzrins Oxalat-Oxidase (EC 1.2.3.4) als spezifischer Katalysator zu Kohlendioxid und Wasserstoffperoxid umgesetzt werden: Das als Reaktionsprodukt entstandene Kohlendioxid kann als unschädlich bzw. vorteilhaft betrachtet werden. Einerseits ist es bei niedrigen pH-Werten als Gas flüchtig, andererseits ist es bei vielen Getränken erwünscht und wird kinstlich zugesetzt.The object is achieved according to the invention in that oxalate and oxygen are converted into carbon dioxide and hydrogen peroxide using the enzrin oxalate oxidase (EC 1.2.3.4) not previously described for oxalate removal as a specific catalyst: The carbon dioxide formed as a reaction product can be regarded as harmless or advantageous. On the one hand it is volatile as a gas at low pH values, on the other hand it is desirable in many beverages and is added kinetically.
Das erzeugte Wasserstoffperoxid wird in vielen Fällen wegen seiner bakteriziden Wirkung willkommen sein. Falls seine Anreicherung unerwünscht sein sollte, kann es iachfolgend mittels des Enzyms Katalase (EC 1.11.1.6) zu Sauerstcff und Wasser umgesetzt werden: Bei gemeinsamer katalytischer Wirkung von Oxalat-Oxidase und Katalase ergibt sich zusammen folgende Reaktionsgleichung für die Entfernung von Oxalat: Es 3011 darauf hingewiesen werden, daß die beiden Enzyme sowohl gleichzeitig als auch sequentiell, d.h. räumlich und/oder zeitlich nacheinander, eingesetzt werden können Als Vorteile der zusätzlichen katalytischen Wirkung von Katalase können angeführt werden; 1. Die Anreicherung von evtl. toxischem Wasserstoffperoxid wird verhindert.The hydrogen peroxide produced will in many cases be welcome for its bactericidal effect. If its enrichment is undesirable, it can be converted into oxygen and water using the enzyme catalase (EC 1.11.1.6): If oxalate oxidase and catalase have a common catalytic effect, the following reaction equation results for the removal of oxalate: It should be pointed out that the two enzymes can be used both simultaneously and sequentially, ie spatially and / or temporally one after the other. Advantages of the additional catalytic effect of catalase can be mentioned; 1. The accumulation of possibly toxic hydrogen peroxide is prevented.
2. Die Umsetzung von Oxalat kann auch in relativ sauerstoffarmen Lösungen vor sich gehen, da die benötigte Sauerstoffmenge halbiert ist.2. The conversion of oxalate can also take place in relatively low-oxygen solutions going on as the amount of oxygen required is halved.
3. Durch Entfernung des Reaktionsproduktes Wasserstoffperoxid erreicht man eine schnelle, quantitative Umsetzung von Oxalat.3. Achieved hydrogen peroxide by removing the reaction product a rapid, quantitative conversion of oxalate.
4. Zur Entfernung einer vorgegebenen Menge Oxalat innerhalb einer bestimmten Zeitspanne genügt eine kleinere Menge Oxalat-Oxidase.4. To remove a given amount of oxalate within one A smaller amount of oxalate oxidase is sufficient for a certain period of time.
5. Neben Wasser en-tsteht nur Kohlendioxid, das bei niedrigem pH-Wert flüchtig ist, d.h, Oxalat wird rückstandslos aus Lösungen entfernt.5. In addition to water, only carbon dioxide is produced, which occurs at a low pH value is volatile, i.e. oxalate is removed from solutions without leaving any residue.
Wenn Oxalat erfindungsgemäß mittels Oxalat-Oxidase und Katalase aus Lösungen entfernt wird, bleiben somit keine störenden oder toxischen Verbindungen zurück.When oxalate is made according to the invention by means of oxalate oxidase and catalase Solutions is removed, so there are no disruptive or toxic compounds return.
Eine Möglichkeit zur Darstellung von Oxalat-Oxidase ist in der Offenlegungsschrift 28 06 371 vom 23.8.79 beschrieben. Auch sind dort Wege zur Bestimmung von Oxalat aufgezeigt, die ebenso wie die von G. Kohlbecker et al. im J. Olin. Chem. Clin, Biochem. 17, 309-313 (1979) angegebene Methode bei den nach stehenden Beispielen eingesetzt wurden.One way of representing oxalate oxidase is in the laid-open specification 28 06 371 of August 23, 1979. There are also ways to determine oxalate shown, which like that of G. Kohlbecker et al. in J. Olin. Chem. Clin, Biochem. 17, 309-313 (1979) given method in the examples below were used.
Die Reaktionsbedingungen können innerhalb weiter Grenzen varîiert werden.The reaction conditions can be varied within wide limits will.
Aufgrund der großen Hitzestabilität der Oxalat-Oxidase sind Temperaturen bis iber 800C möglich. Nach unten wird der Temrecraturbereich nur curch den Gefrierpunkt der wässrigen Lösung beschränkt. Eine zufolge solcher tiefen Temperaturen oder anderer Umstände heralgesetzte Aktivität kann durch den Einsatz vermehrter Enzymmengen oder durch längere Umsatzdauer bis zu mehreren Wochen a:;sgeglichen werden.Due to the great heat stability of the oxalate oxidase, temperatures up to over 800C possible. The temperature range is only reduced by the freezing point limited to the aqueous solution. One due to such low temperatures or another Activity can be reduced due to the use of increased amounts of enzyme or be compensated for by longer sales periods of up to several weeks.
Zumeist wird es nicht nötig sein, reine Oxalat-Oxidase einzusetzen. Präparate mit verminderter Aktivität, die evtl. Katflv lase enthalten, können wirtschaftlich günstig sein, zumal wenn genügend Zeit für die Entfernung von Oxalat zur Verfügung steht. Das ist z.B. bei der nötigen Lagerung von Bier der Fall.In most cases it will not be necessary to use pure oxalate oxidase. Preparations with reduced activity, which may contain catalyst, can be economical be cheap, especially if enough time is available for the removal of oxalate stands. This is the case, for example, with the necessary storage of beer.
Der für die katalytische Wirksamkeit von Oxalat-Oxidase günstigste Wasserstoffionen-Konzentrationsbereich liegt bei pH-Werten zwischen 2,5 und 6,5. Da die meisten Fruchtsäfte, Biere und andere Produkte einen sauren pH-Wert zeigen, kann das Enzym direkt in solche Lösungen eingebracht weiden.The most favorable for the catalytic effectiveness of oxalate oxidase Hydrogen ion concentration range is at pH values between 2.5 and 6.5. Since most fruit juices, beers and other products have an acidic pH, the enzyme can be incorporated directly into such solutions.
Durch den Zusatz katalytischer Mengen Oxalat-Oxidase wird der natürliche Proteingehalt von z.B. Bier von ca. 0,5 - 1 g/l nur unwesentlich erhöht. Trotzdem ist nach dem Reinheitsgebot bzw.By adding catalytic amounts of oxalate oxidase, the natural Protein content of e.g. beer of approx. 0.5 - 1 g / l only marginally increased. Nevertheless is according to the purity law or
Lebensmittelgesetz die Zugabe fremder Substanzen nicht immer möglich. In solchen Fellen können Enzymreaktoren benutzt werden, die kein Protein in die von Oxalat zu befreiende Lösung entlassen. Mögliche Ausführungsformen schlieren beschichtete Filterplatten ein, durch die die Lösung hindurchgeführt wird.Food law, the addition of foreign substances is not always possible. In such pelts, enzyme reactors can be used which do not contain any protein Discharge the solution to be freed of oxalate. Possible embodiments streak coated filter plates through which the solution is passed.
Oxalat-Oxidase kann vorzugsweise auch in Nylonschläuchen immobilisiert werden (R. BAIS et al., Anal. Rohem 52, 508-511 (1980)). Beim Durchströmen solcher Systeme wird die Oxalatkonzentration der Lösung kontinuierlich vermindert.Oxalate oxidase can preferably also be immobilized in nylon tubing (R. BAIS et al., Anal. Rohem 52: 508-511 (1980)). When flowing through such Systems, the oxalate concentration of the solution is continuously reduced.
Die nachstehenden Ausfüh.rungsbeispiele erläutern die Erfindung: 1.a) 0,5 1 Bier (pH 4,2) werden mit 50 Rl (3 U) Oxalat-Oxidase versetzt. Die anfängliche Oxalat-Konzentration von 145 pmol/l sinkt bei 400 nach 5 Tagen auf 83 pmol/l, nach 14 Tagen ist kein Oxalat mehr nachweisbar (Nachweisgrenze der Oxalatbestimmung: 5 Xmol/l).The following exemplary embodiments explain the invention: 1.a) 50 Rl (3 U) oxalate oxidase are added to 0.5 1 beer (pH 4.2). The initial one Oxalate concentration of 145 pmol / l drops at 400 to 83 pmol / l after 5 days No more oxalate can be detected for 14 days (detection limit of the oxalate determination: 5 x mol / l).
1.b) Bei Zugabe von 50 pl Oxalat-Oxidase mit 1 U unter den ansonsten gleichen Voraussetzungen wie 1.a) wird nach 7 Tagen eine Oxalatkonzentration von 91 pmol/l erreicht, nach 14 Tagen sind noch 56 ):imol/l nachweisbar, nach 21 Tagen 33 Fmol/l.1.b) When adding 50 μl oxalate oxidase with 1 U below the otherwise same conditions as 1.a) after 7 days an oxalate concentration of 91 pmol / l reached, after 14 days there are still 56): imol / l detectable, after 21 days 33 fmol / l.
1,c) Werden im Beispiel 1.b) -gleichzeitig 50 µl (13 kU) Katalase zugegeben, so ergeben sich folgende Oxalatkonzentrationen: Nach 7 Tagen 72 pmol/l, nach 14 Tagen 43 umol/l, nach 21 Tagen 25 µmol/l, 2. 10 ml Gemüsesaft (pH 4,1) werden bei 220C mit 25 »1 (1,5 U) Oxalat-Oxidase versetzt. Nach einer Stunde ist die ursprüngliche Oxalatkonzentration von 87 µmol/l unter 5 Rmol/l gesunken.1, c) In example 1.b), 50 µl (13 kU) of catalase are used at the same time added, the following oxalate concentrations result: After 7 days 72 pmol / l, after 14 days 43 µmol / l, after 21 days 25 µmol / l, 2. 10 ml vegetable juice (pH 4.1) at 220C with 25 »1 (1.5 U) oxalate oxidase. After an hour is the original Oxalate concentration of 87 µmol / l dropped below 5 Rmol / l.
3. Zu 10 ml Orangensaft vom pH 3,7 werden bei 220C 10 pl (0,6 U) Oxalat-Oxidase gegeben. Die Oxalatkonzentration sinkt innerhalb einer Stunde von 12,5 Rmol/l unter die Nachweisgrenze von 5 µmol/l.3. 10 μl (0.6 U) of oxalate oxidase are added to 10 ml of orange juice with a pH of 3.7 at 220C given. The oxalate concentration drops from 12.5 Rmol / l within one hour the detection limit of 5 µmol / l.
Claims (7)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE19803030185 DE3030185A1 (en) | 1980-08-07 | 1980-08-07 | Oxalate removal from e.g. foods, beverages or biological fluids - by reaction with oxygen in the presence of oxalate oxidase to give carbon di:oxide and water |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE19803030185 DE3030185A1 (en) | 1980-08-07 | 1980-08-07 | Oxalate removal from e.g. foods, beverages or biological fluids - by reaction with oxygen in the presence of oxalate oxidase to give carbon di:oxide and water |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE3030185A1 true DE3030185A1 (en) | 1982-04-08 |
Family
ID=6109258
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE19803030185 Withdrawn DE3030185A1 (en) | 1980-08-07 | 1980-08-07 | Oxalate removal from e.g. foods, beverages or biological fluids - by reaction with oxygen in the presence of oxalate oxidase to give carbon di:oxide and water |
Country Status (1)
| Country | Link |
|---|---|
| DE (1) | DE3030185A1 (en) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0207039A1 (en) * | 1985-05-22 | 1986-12-30 | Synfina - Oleofina S.A. | Process for the elimination of oxygen from food and oral hygiene compositions and food and oral hygiene compositions containing enzymes to this effect |
| WO2003042380A3 (en) * | 2001-10-05 | 2003-12-18 | Ixion Biotechnology Inc | Materials and methods for reducing oxalate concentrations in fluids |
| WO2009145701A1 (en) * | 2008-05-29 | 2009-12-03 | Nils-Olof Nilvebrant | A method, a package, and a method for making a package, for removing oxalic acid from a beverage |
| US8486389B2 (en) | 1997-05-23 | 2013-07-16 | Oxthera, Inc. | Compositions and methods for treating or preventing oxalate-related disease |
| US8545836B2 (en) | 2005-12-14 | 2013-10-01 | Oxthera, Inc. | Pharmaceutical compositions and methods for treating oxalate-dependent conditions |
| US20140120211A1 (en) * | 2012-10-31 | 2014-05-01 | Erbsloh Geisenheim AG | Use of silicate particles in beverage production |
| US8900575B2 (en) | 2005-12-16 | 2014-12-02 | Oxthera Intellectual Property Ab | Compositions and methods for oxalate reduction |
-
1980
- 1980-08-07 DE DE19803030185 patent/DE3030185A1/en not_active Withdrawn
Non-Patent Citations (2)
| Title |
|---|
| Enzyme Nomenclature 1978, 1979, S. 68 und 106 * |
| Pharmazie in unserer Zeit, H. 2, 1977, S 42-48 * |
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0207039A1 (en) * | 1985-05-22 | 1986-12-30 | Synfina - Oleofina S.A. | Process for the elimination of oxygen from food and oral hygiene compositions and food and oral hygiene compositions containing enzymes to this effect |
| US8486389B2 (en) | 1997-05-23 | 2013-07-16 | Oxthera, Inc. | Compositions and methods for treating or preventing oxalate-related disease |
| US10149866B2 (en) | 2000-02-09 | 2018-12-11 | Oxthera Intellectual Property Ab | Compositions and methods for treating or preventing oxalate-related disease |
| WO2003042380A3 (en) * | 2001-10-05 | 2003-12-18 | Ixion Biotechnology Inc | Materials and methods for reducing oxalate concentrations in fluids |
| CN1578833B (en) * | 2001-10-05 | 2010-05-26 | 奥克斯赛拉公司 | Materials and methods for reducing oxalate concentration in fluids |
| US10653726B2 (en) | 2004-06-15 | 2020-05-19 | Oxthera Intellectual Property Ab | Compositions and methods for treating or preventing oxalate-related disease |
| US8545836B2 (en) | 2005-12-14 | 2013-10-01 | Oxthera, Inc. | Pharmaceutical compositions and methods for treating oxalate-dependent conditions |
| US8900575B2 (en) | 2005-12-16 | 2014-12-02 | Oxthera Intellectual Property Ab | Compositions and methods for oxalate reduction |
| US10272043B2 (en) | 2005-12-16 | 2019-04-30 | Oxthera Intellectual Property Ab | Compositions and methods for oxalate reduction |
| WO2009145701A1 (en) * | 2008-05-29 | 2009-12-03 | Nils-Olof Nilvebrant | A method, a package, and a method for making a package, for removing oxalic acid from a beverage |
| US20140120211A1 (en) * | 2012-10-31 | 2014-05-01 | Erbsloh Geisenheim AG | Use of silicate particles in beverage production |
| US10182586B2 (en) * | 2012-10-31 | 2019-01-22 | Erbslöh Geisenheim AG | Use of silicate particles in beverage production |
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