DE2264527A1 - PROCEDURES FOR THE REFURBISHMENT OF USE ITEMS AND SUITABLE MEANS - Google Patents

Description

UEXKÜLL * STOLBERG PATENTANWÄLTE

DR. J-D. FRHR. by UKXKÜLL OR. ULRICH ORAF «TOLBERO

2 HAMBURG 52

BESELERSTRASSE 4

DIPL.-INQ. JÜRGEN SUCHANTKK

CO-PHA-TEST, cosmetics- (Prio: August 22, 1972

pharmaceutics-test GmbH & Co. KG CH 012441/72 - 10808)

2000 Hamburg 39
Moorfurthweg 9 _ '. Hamburg, September 12th

Process for the sanitation of everyday objects as well as suitable means

The invention relates to a method for the dismantling of articles of daily use made of plastic or similar materials, which with come into contact with the human body. It is known that such objects on their surface a large number of Germs and pathogens, especially bacteria. This phenomenon is of particular importance in the case of objects which are constantly touched by different people without the germs being killed off immediately; this is especially true, for example for doorknobs, toilet seats and telephones, while at least toilets are subject to frequent disinfection the same is by no means true of telephone sets, although, for example, coin operated telephones or telephone sets in offices

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226-527.

constantly used by a wide variety of people, including both be contaminated with germs of all kinds through touch with the hand as well as through the transfer of droplets when speaking.

In all of the aforementioned cases, it is possible to achieve a largely complete destruction of all germs by disinfection with a known disinfectant. All disinfectants listed in the list of recommendations from the German "Bunde s ^ e sundhe it, including the disinfectants listed, in particular, for example, 70 to 80 vol. Ethyl alcohol, 50 to 60, are suitable for this vol. isopropyl alcohol, phenol derivatives and aldehyde-containing agents, especially agents based on formaldehyde. In terms of disinfection or disinfection, these agents are optimally effective because they have a bactericidal and sometimes even virucidal effect. However, the antibacterial effect is limited to a very short time, so that the treated object can be contaminated again practically immediately after disinfection. Attempts have been made to achieve an antiseptic effect over a longer period of time by emulsifying the active ingredients in fats, for example. Such preparations are not, however, for the purposes of interest here It is practical because it affects the human body when the objects are touched with the hands or other parts of the body Skin over, so that the effect is extremely limited in time. In addition, it is usually not possible to treat objects of daily use with greasy agents, because the adhesion of dust would result in inappropriately soiling.

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Also experiments with surface-active substances, in particular quaternary ammonium compounds, which are antibacterial over a long period of time are effective did not lead to success. A longer lasting effect is probably not achieved because of this Active ingredients are water-soluble, so that on contact with sweaty skin they remove from the surface of the treated objects removed.

In contrast, the invention is primarily concerned with the task based on the development of a process by means of which articles of daily use, which often come into contact with the human skin come, not just disinfected once, but beyond that for the period between two disinfections can be equipped effectively antibacterial in this way a to prevent the transmission of pathogens. In other words, the state reached by the disinfection should be over maintained for a longer period of time and. on. these. Way to prevent the transmission of infectious diseases. '* - ..

The invention accordingly relates to a method for the surface assembly of articles of daily use made of plastic or similar materials which come into contact with the human body and which is characterized in that • one, if necessary, cleaned and through

40981 α / 1 ΟΛΟ

Disinfection of sterilized items for a longer period of time Protection against contamination treated with an agent which has an essentially water-insoluble, bacteriostatic effect which is absorbed onto the surface of the object to be treated Contains substance or combination of active substances.

It is essential for the method according to the invention that the Objects of use are not only treated with a bactericidal agent in the sense of disinfection, but that In addition, a bacteriostatic treatment is carried out, whereby the actual sanitation is achieved.

So far, bacteriostatic agents have essentially only found use in medicine and cosmetics . In the tables kosme industry such compounds are incorporated into respective formulations which are used as deodorant soaps, aerosol sprays or similar products on the market. What is essential for the longer-lasting deodorant effect of these preparations is the fact that the compounds are absorbed onto the human skin and are deposited there in the sense of a depot. A similar depot effect is seen with other materials of natural origin, e.g. B. observed with line or cotton and also with animal full. Investigations into the impregnation of substances made from these materials by washing them with washing powders containing bacteriostatic substances were carried out.

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It was found that it is possible by adding
to reach from 1 to 3 wt.% of bacteriostatic agents to the washing-active substances in the washing process an antibacterial equipment. Appropriate equipment from
Fabrics made from synthetic fibers: On the other hand, it has proven impossible because the funds do not absorb. -

Completely surprisingly, on the other hand, it was found that certain bacteriostatically active compounds are absorbed onto plastics and have a long-lasting bacteriostatic activity there
unfold. As particularly suitable bacteriostatic agents
In the context of the present invention, halophenol compounds have been found. Multiple chlorinated ones are preferred
Phenolic compounds, especially the 2, U, H * -TricYilor-a * -hydroxy diphenyl ethers. Combinations of these compounds with di-, tri- and tetrahalosalieylanilides, for example 3,3 '» ^I », 5 ^I -tetraehlorsalicylanil, V , Ί', 5-trichloro- and 3, Ji ^t , 5-tribromosalicylanilide and their are particularly suitable Mixtures or with essential oils, as these combinations show a synergistically increased effect. However, the compounds mentioned can also be used alone *

Other useful water-insoluble bacteriostatic agents include 1,6-bis (p-chlorphenyldiguaniao) hexane, 3,4, ¹ I ¹ -Trichlorcarbanilid, 3-Trifluorniethyl- ^J! _> k '-diohlor-S.N'-diphenyl urea and similar compounds known per se, which can be used alone or in a mixture with the aforementioned.

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With these agents, a long-lasting antibacterial impregnation with a depot character is achieved, which remains effective even with frequent contact with human skin. Both immediately after the treatment and after repeated washing with water or after frequent contact, the contact growth index (KWI) according to the determination method described in detail below is between 0 and 1.

0 & 6 '\ 0/1 0 4 0
BATH

To carry out the method according to the invention, the objects are first cleaned of coarse dirt, if necessary. The objects must then be subjected to a disinfecting treatment with bactericidal agents in order to achieve a fine, largely aseptic condition. Agents based on formaldehyde have proven to be particularly suitable and effective. This is followed by treatment with the dissolved bacterium, which according to the invention may be practically insoluble in water and draws onto the object to be assambled8. The method according to the invention can, however, not only be carried out in two stages, as described above, but also in one stage if an agent is used which has a bactericidal and thus disinfecting effect at the same time and which has a long-lasting bacteriostatic effect. This can be done advantageously by using the bacteriostatic agent in the form of a solution in a high-percentage alcohol, which has a bactericidal effect and initially kills all germs, but then quickly evaporates. Aliphatic alcohols with 1 to 3 carbon atoms are particularly suitable; In particular, very good results were achieved with at least 70% aqueous ethanol and at least 50% aqueous isopropanol.

It was also found that the effect of the invention spent funds can be increased if you give them a add a certain amount of a non-ionic emulsifier. Preferred

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Emulsifiers are polyoxyethylene sorbitan esters and fatty acid alkyl esters. Isopropyl myristate, which additionally consists of the objects treated according to the invention, is particularly preferred Plastic gives increased shine. However, other non-ionic emulsifiers can also be used with advantage.

The invention further comprises means for carrying out the Assanierungsverfahrens ^ r ~ which-by, a content of 0.001 to 0.05, in particular 0.002 to 0.02 wt.% Of a water-insoluble bacteriostats and optionally 0.1 to 2.0 in particular 0.5 to 1.0 wt. I of a non-ionic emulsifier in an aqueous, at least 50 vol. Ji-igen aliphatic C. to C, alcohol is labeled. Contents of more than 0.05 wt.? Bäkteriostat do not make economic sense. If desired, the agent can also be used in the form of an aerosol spray. With this agent, an effective sanitation can be achieved for a longer period of time, whereby, due to the content of bactericidally effective high-percentage alkanol, prior disinfection may be dispensed with. Based on previous knowledge, it could not be foreseen in any way that such agents would be absorbed, for example, on plastic surfaces such as the material of telephone sets (acrylonitrile / butadiene / styrene polymers) and develop a long-lasting effect. For this reason, so far only a disinfection is known, but not a dismantling of everyday objects.

Proof of the effectiveness of the treatment according to the invention

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succeeded by determining the contact growth index (KWI). The bacteriological examination methods that have been used up to now, For example, the diffusion test, 'are not suitable for this, since the applied bacteriostatic substances as a result of the The lack of water solubility does not diffuse into the nutrient medium and thus does not lead to an inhibition zone. Rather, it had to be a new investigation method are developed, which is based in principle on the fact that the substances to be investigated on a Carrier are applied, whereupon the same is incorporated into a solid nutrient medium so that the carrier on its surface is just overlaid with nutrient agar, so that on one side an inoculation and thus a bacterial growth is possible, on the other hand, the layer thickness is only a fraction of one Millimeters. In this way there is almost direct contact between the inoculated carrier plate during the incubation the substance to be tested and the types of bacteria applied. With this method the control of the bacterial growth is thereby given that optimal bacterial growth must take place on the nutrient agar, which is not in contact with the carrier. The procedure at the same time enables a conclusion about the water solubility of the examined substance, because bacterial inhibition beyond the area of the wearer - i.e. in the control zone on it indicate that the substance to be investigated due to its water solubility during the incubation in the surrounding Culture medium has diffused into it. Here is the one after the incubation

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assessed KWI according to the following scale:

0 = total inhibition on the entire contact area ~~

1 = minimal growth at the edge of the contact area

2 = very isolated microcolonies on the contact area

3 = a small number of microcolonies on the contact area ή = optimal growth on the contact area corresponding to that in the control zone

The following example is intended to provide a more detailed explanation of the invention.

example

The following bacteriostatically effective agents were first produced for the subsequent tests, using 70% ethyl alcohol by volume as the solvent in each case:

Agent A: 0.02 wt. * 2, * !, 4'-Trichloro-2'-hydroxydiphenyl ether Agent B: 0.01 wt.? 2,! », Ή'-trichloro-2 ^{f -hydroxydiphenyl ether}

(5% 3,5-dibromosalicylanilide an impurity) 0.01% wt. 3, ¹ * ', 5-tribromosalicylanilide

Medium C: 0.02 wt. 2, H, 4'-trichloro-2 · hydroxydiphenyl ether and

1.0 wt% isopropyl myristate means. D: 0.01 Gew.JS 2, U, 4'-trichloro-2'-hydroxy diphenyl ether

0.01 wt. * 3,4 ', 5-tribromosalicylanilide (with 5% 3,5-dibromosalicylanilide as an impurity) and

% 0.1 wt high molecular alkyldimethylbenzylammonium: 1.0 wt% isopropyl myristate agent E..

chloride

(Comparison)

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BAOORfGiNAC

The antibacterial depot effect of the above agents aur the Surfaces of telephones made of acrylonitrile / butadiene / styrene polymers (Novodur) was examined by determining the KWI. For this purpose, square plates with an edge length of 2 × 2 cm were made from the Sawn out telephone sets and then treated with the means by vigorously rubbing the surface with a cotton swab which was soaked with the solution to be examined. The KV / I was determined "as described above, with 10 Staphylococcus aureus-, 10 Staphylococcus epidermidis-, 10 Escherichia coli-, 10 Proteus spec-, 10 Pseudomonas aeruginosa- and 10 Aerobacter Klebsiella strains were tested.

Test 1

First of all, the plastic platelets treated with agents A to E were applied determined by KWI after air drying. A second series, which had been pretreated in the same way, was then placed in a shaker and shaken in distilled water for 10 minutes, whereupon the platelets are placed in a new vessel were transferred with distilled water. This treatment was repeated six times, changing the water, whereupon the KWI was determined. In the first series, a KWI of 0 was found for all 5 agents tested; on the other hand emerged in the case of the second Series for the means E treated plastic platelets a KViI of 1J, for those treated with means A to D, however, a KV / I of

Test 2 -

After the above studies, an excellent effect had shown the treatment according to the invention, further investigations on the depot character of the impregnation were carried out in this way.

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assumed that after treating the surface of the plastic platelet with the cotton swab soaked with the agent to be tested, the platelets are incorporated six times in succession in nutrient agar and were taken out again; only after the last incorporation was inoculated with the various bacterial strains. Despite the necessary removal of the agar layer covering the platelet after each incorporation, Agent A showed treated Platelets still had a KWI of 1, while a KWI of O was found for agents B, C and D. In contrast, the KV / I was for means E at 4.

Test 3 .

Another series of tests was carried out with the aim of imitating the conditions in practical use as naturally as possible. For this purpose, the surfaces of the platelets were rubbed, as described above, with cotton swabs which had been soaked with the solutions to be examined. A total of 100 fingers were then pressed onto the treated surface before the platelets were incorporated into the nutrient agar. However, only one of the culture medium plates treated in this way was inoculated with bacteria, while another was used for comparison. The plate pairs were each ^{incubated for 20 hours at 37 °} C., whereupon the KWI determined for the inoculated plate, but in the case of the non-incubated plate the attack of the contamination germs applied to the plates by touching the fingers could be observed. Agent D showed the best effectiveness, but the other agents according to the invention also proved to be fully effective. The contamination germs applied by touching could not multiply on the platelets. in the

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For some, a KWI of 1 to 2 was determined for composition A, a KWI of 0 to 1 for B, C and D and a KWI for E

of 4. "''.

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Claims (1)

Patent application 1. Process for the surface cleaning of objects of daily use Made of plastic and similar materials which come into contact with the human body, characterized in that the possibly cleaned and disinfected aseptic objects for longer-lasting protection against Contamination treated with an agent that has a substantially water-insoluble, on the surface of the treated Contains bacteriostatic substance or combination of active substances that attract the object. 2. The method according to claim 1, characterized in that the disinfection and contamination protection by using a performs both bactericidal and bacteriostatic agent at the same time. 3. The method according to claim 1 and 2, characterized in that the bacteriostatic active ingredient used is halogenated phenol compounds and / or halosalicylanilides, preferably 3.3S ¹ US ¹ tetrachlorosalicylanilide and / or V j ¹ * ¹ , 5-trichlorosalicylanilide and / or tribromosalicylanilide . k. The method according to claim 1 to 3, characterized in that an agent is used which, in addition to the bacteriostat, also A0S810 / 1040 226452? a non-ionic emulsifier, preferably isopropyl myristate and / or contains polyoxyethylene sorbitan esters. 5. The method according to claim 1 to 4, characterized in that the agent in the form of a solution in a 50 to 100 vol. JS aliphatic C ₁ to C, alcohol is used. 6. Means for carrying out the method according to claims 1 to 5, characterized by a content of 0.001 to 0.05 wt. % Of a water-insoluble bacteriostatic in an aqueous, "at least 50 vol.Jf-igen aliphatic C. to (! .. -Alcohol. 7. Means according to claim 6, characterized in that it 0.1 to 2.0 wt. contains a non-ionic emulsifier. 8. Means according to claim 6 or 7> characterized in that as a bacteriostatic a halogenated phenol compound or a synergistic combination of the same with a halosalicylanilide and / or an essential oil. 9 · Means according to patent claims 6 to 8, characterized in that it is in the form of an aerosol spray. 10. Agent according to claim 6 to 9, characterized in that it additionally contains a disinfectant as a bactericidal active ingredient . ■ / 409810/1040