DE19905163A1 - Organs for transplanting are stored after removal from donor in liquid medium under high pressure at a low temperature which is above the freezing point of medium at prevailing pressure - Google Patents
Organs for transplanting are stored after removal from donor in liquid medium under high pressure at a low temperature which is above the freezing point of medium at prevailing pressureInfo
- Publication number
- DE19905163A1 DE19905163A1 DE19905163A DE19905163A DE19905163A1 DE 19905163 A1 DE19905163 A1 DE 19905163A1 DE 19905163 A DE19905163 A DE 19905163A DE 19905163 A DE19905163 A DE 19905163A DE 19905163 A1 DE19905163 A1 DE 19905163A1
- Authority
- DE
- Germany
- Prior art keywords
- pressure
- organs
- liquid medium
- removal
- transplanting
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
- 238000007710 freezing Methods 0.000 title claims abstract description 10
- 230000008014 freezing Effects 0.000 title claims abstract description 10
- 239000007788 liquid Substances 0.000 title claims abstract 5
- 210000000056 organ Anatomy 0.000 title abstract 4
- 238000000034 method Methods 0.000 claims abstract description 8
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims 1
- 238000001816 cooling Methods 0.000 abstract 1
- 238000000354 decomposition reaction Methods 0.000 description 4
- 230000015572 biosynthetic process Effects 0.000 description 2
- 239000013078 crystal Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 230000002427 irreversible effect Effects 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 238000001356 surgical procedure Methods 0.000 description 1
- 238000002054 transplantation Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/10—Preservation of living parts
- A01N1/16—Physical preservation processes
- A01N1/165—Pressure processes, e.g. following predefined pressure changes over time
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/10—Preservation of living parts
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
Description
In der derzeitigen Transplantationschirurgie stellt sich das Problem, daß zwischen der. Entnahme eines Transplantates vom Spenderorganismus und der Transplantation in den Empfängerorganismus eine Zeitspanne liegt, die u. a. davon abhängt, wie weit der Spender und der Empfänger räumlich getrennt sind. Da es mittlerweile weltweite Datenbanken gibt, in denen die Gewebedaten von Patienten gespeichert sind, die ein Transplantat benötigen, kann die Entfernung zwischen Spender und Empfänger bis zu 20 000 km sein. Diese Entfernung zu überbrücken und das Transplantat zum Empfänger zu bringen, kann somit bis zu 20 h dauern. Da jedoch bereits während der Entnahme eines Transplantates aus dem Spenderorganismus die biologische Zersetzung des Transplantates beginnt, kann nicht gewährleistet werden, daß das Transplantat bei Eintreffen am Ort des Empfängers noch in den Empfängerorganismus transplantiert werden kann, wenn die biologische Zersetzung schon so weit fortgeschritten ist, daß das Transplantat irreversible Schädigungen erlitten hat. Aus diesem Grund werden Transplantate gekühlt gelagert und transportiert. Die Herabsetzung der Temperatur verlangsamt die im Transplantat ablaufenden Prozesse der biologischen Zersetzung. Die Temperatur der Transplantate darf jedoch nicht unter den Gefrierpunkt des Transplantates fallen, der in einem Bereich von -5 bis 0°C liegt, da es sonst in den Zellen des Transplantates zur Eiskristallbildung kommen würde, was die Zellen wiederum zerstören könnte. Die Zugabe von gefrierpunkterniedrigenden Stoffen, wie Salz oder Alkohol, verbietet sich, da diese Stoffe das Transplantat schädigen würde. Das erfindungsgemäße Verfahren ermöglicht eine Absenkung des Gefrierpunktes von Transplantaten ohne Zugabe von Stoffen. Durch das erfindungsgemäße Verfahren können Transplantate auf Temperaturen unter -20°C abgekühlt werden, ohne daß es dabei zu einer Eiskristallbildung in den Zellen der Transplantate kommt. Durch diese Temperaturerniedrigung verlangsamen sich die Prozesse der biologischen Zersetzung der Transplantate. Der Zeitraum zwischen Entnahme und Reimplantation der Transplantate kann dadurch verlängert werden.In current transplant surgery, there is the problem that between the. Removal of a transplant from the donor organism and the Transplantation in the recipient organism is a period of time that u. a. from that depends on how far the donor and the recipient are spatially separated. Because it meanwhile there are worldwide databases in which the tissue data of patients that need a graft can be the distance between Donors and recipients up to 20,000 km. To bridge this distance and taking the graft to the recipient can take up to 20 hours. There however, already during the removal of a graft from the Donor organism can begin the biological decomposition of the graft no guarantee can be given that the graft will arrive at the site of the Recipient can still be transplanted into the recipient organism if the biological decomposition is so advanced that the graft has suffered irreversible damage. This is why grafts are used stored and transported refrigerated. The decrease in temperature slows down the biological decomposition processes taking place in the graft. The However, the temperature of the grafts must not be below the freezing point of the Grafts fall, which is in a range of -5 to 0 ° C, otherwise it is in the Cells of the graft would come to ice crystal formation, causing the cells again could destroy. The addition of freezing point depressants, such as Salt or alcohol is prohibited, as these substances would damage the graft. The method according to the invention enables the freezing point to be lowered of grafts without adding substances. By the invention Procedures, grafts can be cooled to temperatures below -20 ° C, without the formation of ice crystals in the cells of the grafts. This lowering of the temperature slows down the processes of the biological decomposition of the grafts. The period between removal and Reimplantation of the grafts can be extended.
Dem erfindungsgemäßen Verfahren liegt zugrunde, daß die Gefriertemperatur von Wasser bei Erhöhung des Druckes sinkt (siehe Zeichnung 1). Bei einem Druck von ca. 2000 bar liegt die Gefriertemperatur bei ca. -22°C. Die Transplantate werden direkt nach Entnahme aus dem Spenderorganismus in eine geeignete wässrige Lösung verbracht und in flexible Behältnisse verschlossen. Anschließend erfolgt eine Druckerhöhung auf 200 bis 2500 bar. Während der Druckerhöhung oder nach Erreichen des Druckes wird das Transplantat soweit abgekühlt, daß die Temperatur immer noch über der zu dem Druck gehörigen Gefriertemperatur liegt.The inventive method is based on the freezing temperature of Water drops when the pressure is increased (see drawing 1). At a pressure of The freezing temperature is approx. 2000 bar at approx. -22 ° C. The grafts will immediately after removal from the donor organism into a suitable aqueous Solution spent and sealed in flexible containers. Then there is one Pressure increase to 200 to 2500 bar. During the pressure increase or after When the pressure is reached, the graft is cooled until the temperature is still above the freezing temperature associated with the pressure.
Claims (2)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE19905163A DE19905163A1 (en) | 1999-02-08 | 1999-02-08 | Organs for transplanting are stored after removal from donor in liquid medium under high pressure at a low temperature which is above the freezing point of medium at prevailing pressure |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE19905163A DE19905163A1 (en) | 1999-02-08 | 1999-02-08 | Organs for transplanting are stored after removal from donor in liquid medium under high pressure at a low temperature which is above the freezing point of medium at prevailing pressure |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE19905163A1 true DE19905163A1 (en) | 2000-08-10 |
Family
ID=7896840
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE19905163A Withdrawn DE19905163A1 (en) | 1999-02-08 | 1999-02-08 | Organs for transplanting are stored after removal from donor in liquid medium under high pressure at a low temperature which is above the freezing point of medium at prevailing pressure |
Country Status (1)
| Country | Link |
|---|---|
| DE (1) | DE19905163A1 (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6931864B2 (en) | 2000-12-07 | 2005-08-23 | Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. | Cryostorage method and device |
| US7458285B2 (en) | 2003-06-26 | 2008-12-02 | Fraunhofer-Gesellschaft Zur Foerderung Der Angewandten Forschung E.V. | Device for cryopreservation of samples and method for producing the device |
| EP4106520A4 (en) * | 2019-07-05 | 2024-02-21 | Cryostasis Inc. | METHOD AND APPARATUS FOR STORAGE OF BIOLOGICAL MATERIALS |
-
1999
- 1999-02-08 DE DE19905163A patent/DE19905163A1/en not_active Withdrawn
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6931864B2 (en) | 2000-12-07 | 2005-08-23 | Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. | Cryostorage method and device |
| EP2302351A1 (en) | 2000-12-07 | 2011-03-30 | Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. | Method and apparatus for cryogenic storage of probes |
| US7458285B2 (en) | 2003-06-26 | 2008-12-02 | Fraunhofer-Gesellschaft Zur Foerderung Der Angewandten Forschung E.V. | Device for cryopreservation of samples and method for producing the device |
| EP4106520A4 (en) * | 2019-07-05 | 2024-02-21 | Cryostasis Inc. | METHOD AND APPARATUS FOR STORAGE OF BIOLOGICAL MATERIALS |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 8139 | Disposal/non-payment of the annual fee |