DE19649097A1 - Pharmaceutical or cosmetic compositions comprising lipase(s) - Google Patents

Abstract

A pharmaceutical or cosmetic composition (I) comprising \- 1 lipase is new.

Description

The invention relates to uses of lipases and preparations, containing these substances.

Lipases and their uses for a variety of purposes are known.

Lipases are enzymes from the group of hydrolases (especially the esterases), specifically cleaving triglycerides into glycerol and fatty acids; this lipolysis This process is activated in humans by bile acids. lipases are digestion-physiologically particularly important and found in almost all Animal species, but also in oil-containing plant seeds (eg Ricinus) u. in micro organisms. They are particularly abundant in pancreas, intestinal wall and the like. liver in front. The pH optimum is at pH 5-9. The pancreatic lipase only splits the While the gut wall enzyme 2-acyl glycerine attacks. Mechanistically, catalysis often involves an L-serine residue a role. Lipases become therapeutic in disorders of fat digestion used u. also serve for the analytical determination of triglycerides.

It is already known microorganisms on surfaces by microbicides kill or rinse with cleansers to get their way this way Reduce number on the surface. Both methods have known  Disadvantage. So z. B. disinfectants damage the surfaces and Detergents are often insufficient in their effect.

The object of the invention was, therefore, preparations and a gentle and to create an effective method of managing the number of Microorganisms, viruses, parasites and protozoa low on surfaces maintain or reduce or prevent microorganisms, viruses, Parasites and protozoa adhere to surfaces.

These tasks are solved by the use of lipases and Preparations containing lipases.

The invention relates to pharmaceutical or cosmetic Zuberei tions containing one or more compounds the group of lipases.

Preference is given to topical or topically applied pharmaceutical or cosmetic preparations.

The invention also relates to the use of a compound or several compounds from the group of lipases as antiadhesive agents, especially against microorganisms, viruses, parasites and protozoa.

With the terms "antiadhesive" and "antiadhesive effect" of the invention According to active ingredients, it is meant that the adhesion of microorganisms, viruses, Parasites and protozoa are reduced or suspended on surfaces.

The invention also relates to the use of a compound or several compounds from the group of lipases as antiadhesive agents against microorganisms, viruses, parasites and protozoa as part of Preparations.  

The invention also relates to preparations with a content of one Compound or compounds from the group of lipases with anti Adhesive action against microorganisms, viruses, parasites and protozoa.

The antiadhesive agents and preparations according to the invention cause that on surfaces only small and no disturbing accumulations of microorganisms, viruses, parasites and protozoa, or they displace already adhering microorganisms, viruses, parasites and Protozoa from the surface and thus reduce their number. You can also be used prophylactically.

The stated objects are achieved according to the invention. The invention Active ingredients according to the invention have the aforementioned effects Microorganisms, viruses, parasites and protozoa and are used to treat the suitable disorders and diseases.

Well suitable lipases according to the invention can

• a) of plant origin,
• b) of animal origin,
• c) be of bacterial origin or
• d) come from mushrooms.

Preference is given to the following lipases, for each of which the origin and a source of supply are given.

• a) of plant origin
  Lipase type I Wheat Germ (Sigma) Lipase type IA Wheat Germ (Sigma) lipase Wheat germ (Fluka)
• b) of animal origin
  Lipase type II (Steapsin) Porcine Pancreas (Sigma) lipase Human Pancreas (Sigma) Lipase type VI-S Porcine pancreas (Sigma) Lipoprotein lipase bovine milk (Sigma) lipase pig pancreas (Fluka)
• c) of bacterial origin
  Lipase type XII Chromobacterium viscosum (Sigma) Lipase type XIII Pseudomonas species (Sigma) lipase Thermus thermophilus (Fluka) lipase Thermus aquaticus (Fluka) lipase Thermus flavus (Fluka) Phosphatidylinositol phospholipase C Bacillus cereus, 1-phosphadityl-D-myoinositol phosphohydrolase (Boehringer Mannheim) Phospholipase D Streptomyces chromofuscus (Boehringer Mannheim)
• d) from mushrooms
  lipase Candida rugosa (Sigma) Lipase type VII Candida rugosa (Sigma) Lipase type XI Rhizopus arrhizus (Sigma) Lipase type VII-A Candida rugosa (Sigma) lipase Aspergillus niger (Fluka) lipase Aspergillus oryzae (Fluka) Lipase A u. B Candida antarctica (Fluka) lipase Candida cylindracea, triglycerol hydrolase (Fluka) lipase Candida lipolytica (Fluka) lipase Candida utilis (Fluka) lipase Mucor javanicus (Fluka) lipase Mucor miehei (Fluka) lipase Penicillum roqueforti (Fluka) lipase Pseudomonas cepacia (Fluka) lipase Pseudomonas fluorescens (Fluka) lipase Rhizomucor miehei (Fluka) lipase Rhizomucor arrhizus (Fluka) lipase Rhizomucor delemar (Fluka) lipase Rhizomucor niveus (Fluka)

The preparation of the active compounds according to the invention is carried out by the person skilled in the art common, usual methods.

Active ingredients according to the invention are commercially available or known or can be obtained by known methods.

Preparations, in particular topical preparations, eg. B. cosmetic and dermatological preparations, with the active compounds according to the invention can these z. In amounts of from 0.001 to 20% by weight, preferably from 0.01 to 10% by weight, but especially 0.1 to 5 wt .-%, each based on the total weight of the preparations. In particular, these quantities also apply in each case for the individual components of the combinations.

Surprisingly, it has been found that the active compounds according to the invention and preparations containing them, the adhesion, d. H. the assets z. B. the Microorganisms, viruses, parasites and protozoa to adhere to surfaces, reduce their usual number to such areas, or also that no or no significant amounts of micro organisms, viruses, parasites and protozoa.  

Such surfaces are z. B. Organ outer surfaces or organ surfaces, especially from the skin or mucous membrane and body cavities or organ cavities, wound cavities or the eye, or the eye socket, the eyes cornea and the area between eyeball and eyelid.

The healthy warm-blooded organism, especially the healthy person Liche skin is colonized with a variety of non-pathogenic microorganisms. This so-called microflora of the skin is not only harmless, it provides one important protection against opportunistic or pathogenic germs.

Bacteria belong to the prokaryotic unicellular organisms. You can roughly after their shape (sphere, cylinder, curved cylinder) and after the construction their cell wall (gram positive, gram negative) can be distinguished. finer Subdivisions also take into account the physiology of the organisms. So exist aerobic, anaerobic and facultative anaerobic bacteria. Some Individuals are more medicinal in their capacity as pathogenic germs Meaning, others are completely harmless.

Against bacteria active substances have been known for some time. The Term "antibiotics" for example, not all antimicrobial effective Substances applicable, can be dated to the year 1941, although the first findings on penicillin were already found in 1929. Antibiotics in the modern sense are not for all medical, especially not at all suitable for cosmetic applications, as often the warm-blooded Organism, that is about the diseased patient, when applied to any Is impaired in its metabolic functions.

An object of the present invention was therefore the prior art in enrich this direction, in particular, therefore, substances available which are against Gram-positive and / or Gram-negative bacteria are antiadhesive effect, without that with the application of the substances  unacceptable impairment of the health of the user would.

Gram-negative bacteria are, for example, Escherichia coli, Pseudomonas species and enterobacteriaceae, such as Citrobacter.

Gram-positive bacteria also play a role in cosmetics and dermatology. In the case of impure skin, for example, among other influences bacterial secondary infections of etiological significance. One of most important microorganisms associated with impure skin, is Propionibacterium acnes.

Impure skin and / or comedones affect the wellbeing of the Be but even in light cases. Because practically everyone or every youth If it is affected by blemished skin of any kind, it persists many persons need to remedy this condition.

A particular object of the present invention, it was a against Impure skin or Propionibacterium acnes effective substance or substance to find a combination.

The present invention relates in a further embodiment cosmetic Deodorants. Such formulations serve to treat body odor eliminate, which arises when the in itself odorless fresh sweat through especially gram-positive microorganisms is decomposed. The usual Cosmetic deodorants are based on different active principles.

Known and common are both liquid deodorants, for example Aerosol sprays, roll-ons and the like as well as solid preparations, for example, deodorant sticks ("sticks"), powders, powder sprays, intimate cleaners etc.  

In so-called antiperspirants can be caused by astringents - predominantly Aluminum salts such as aluminum hydroxychloride (Aluchlorhydrat) - the emergence the sweat be prevented. Apart from the denaturing of Skin proteins, however, access the substances used, depending on their Dosage, drastic in the heat balance of the axillary region and should if necessary in exceptional cases.

By using antimicrobial substances in cosmetic deodorants The bacterial flora on the skin can be reduced. It should ideally only the odor causing microorganisms are effectively reduced. In In practice, it has been found that the entire microflora of the skin can be affected.

The sweat flow itself is not affected, in the ideal case, only the microbial decomposition of sweat temporarily stopped.

The combination of astringents with antimicrobial substances in one and the same composition is in use. The disadvantages of both However, drug classes are not completely complete in this way remove.

Finally, body odor can also be covered by fragrances, a Method, the least the aesthetic needs of the consumer is fair, since the mixture of body odor and perfume fragrance rather unan smells pleasant.

However, most cosmetic deodorants, as well as the Most cosmetics overall, perfumed, even if they are deodorant Active ingredients include. Perfuming can also serve consumers increase the acceptance of a cosmetic product or a product to give a certain flair.  

The perfuming of active ingredient-containing cosmetic agents, in particular Cosmetic deodorants, however, is often problematic because Actives and perfume ingredients occasionally react with each other and can render each other ineffective.

Deodorants should fulfill the following conditions:

• 1) They should cause a reliable deodorization.
• 2) The natural biological processes of the skin must not be affected by the Deodorants are impaired.
• 3) The deodorants must in case of overdose or other not determin harmless to the intended use.
• 4) They should not be on the skin after repeated use accumulate.
• 5) They should work well into common cosmetic formulations to let.

Another object of the present invention was therefore cosmetic To develop deodorants that are not the disadvantages of the prior art respectively. In particular, the deodorants should be the microflora of the skin largely spare, but the number of microorganisms responsible for the body odor, reduce it selectively.

Furthermore, it was an object of the invention to provide cosmetic deodorants develop, which are characterized by good skin compatibility. Under no circumstance the deodorizing principles should accumulate on the skin.

Another task was to develop cosmetic deodorants, which with the largest possible variety of conventional cosmetic auxiliary and additive harmonize, especially with those in deodorising or antiperspirant formulations of significant perfume ingredients.  

Yet another object of the invention was to provide cosmetic deodorants To provide, which over a longer period, in the Order of magnitude of at least half a day, are effective without their Noticeable effect.

Finally, an object of the present invention was deodorising To develop cosmetic principles that are as universal as possible various forms of administration of cosmetic deodorants incorporated without one or a few specific dosage forms to be committed.

Fungi, also fungi [fungus = Latin fungus], Mycota [mukhσ = Greek fungus] or Mycobionts, count in contrast to the bacteria to the Eukaryotes. Eucaryotes are living things, whose cells (Eucyten) in the counter to those of the so-called procaryotes (Procytes) via a Nuclear envelope and nuclear membrane separated from the rest of the cytoplasm nucleus feature. The nucleus contains the genetic information stored in chromosomes.

Representatives of mycobionts include, for example, yeasts (Protoascomycetes), Molds (Plectomycetes), mildew (Pyrenomycetes), the downy mildew (Phycomycetes) and the stem fungi (Basidiomycetes).

Mushrooms, not even the Basidiomycetes, are not plant organisms but like these a cell wall, cell-filled vacuoles and a microscopic highly visible plasma flow. They contain no photosynthetic pigments and are C-heterotrophic. They grow under aerobic conditions and win Energy through oxidation of organic substances. Some representatives, for example wise yeasts, however, are optional anaerobes and for energy production enabled by fermentation processes.

Dermatomycoses are diseases in which certain types of fungi, in particular Dermatophytes, penetrate the skin and hair follicles. The symptoms of  Dermatomycoses are, for example, vesicles, exfoliation, rhagades and Erosion, usually associated with itching or allergic eczema.

Dermatomycoses can be divided essentially into the following four groups dermatophytics (eg epidermophytia, favus, microspore, trichophytia), Yeast mycoses (eg, pityriasis and other myrotic pityrosporum-related, Candida infections, blastomycosis, buses Buschke disease, torulosis, Piedra alba, torulopsidosis, trichosporosis), mold mycoses (eg aspergillosis, Cephalosporidosis, phycomycosis and scopular isopidemias), systemic mycoses (e.g. Chromomycosis, coccidiomycosis, histoplasmosis).

The pathogenic and facultative pathogens include, for example from the group of the yeast Candida kinds (eg Candida albicans) and those of the Family Pityrosporum. Pityrosporum species, in particular Pityrosporum ovale, are for skin conditions such as pityriasis versicolor, seborrhoea in the forms Seborrhoea oleosa and seborrhoea sicca, which are mainly called seborrhoea capitis (= dandruff), seborrheic dermatitis and pityrosporum folli to blame kulitis. Involvement of Pityrosporum ovale The development of psoriasis is discussed by experts.

All areas of human skin can be affected by dermatomycoses become. Dermatophytoses almost exclusively affect the skin, hair and nails. Yeast mycoses can also infect mucous membranes and internal organs, system mycoses regularly extend to whole organ systems.

Particularly often the areas of the body are affected by which Clothing, jewelry or footwear can dampen moisture and heat. So The athlete's foot is one of the best known and most widely used Dermatomycoses. Particularly unpleasant are further fungal diseases of Finger and toenail areas (onychomycoses).

Furthermore, superinfections of the skin by fungi and bacteria are not uncommon.  

In case of primary infection, d. h., the normal germination of the skin, entering new infection with high germ counts of one or more often physiological agents, such as staphylococci, but often also Unphysiological pathogen, for example Candida albicans, may be included Coincidence of unfavorable influences a "superinfection" of the afflicted Skin occur. The normal microflora of the skin (or other Körperor goose) is literally overgrown by the secondary pathogen.

Such superinfections may, depending on the particular germ, in favorable cases in unpleasant skin conditions (Juck irritant, unpleasant external appearance). In unfavorable running However, they can lead to extensive destruction of the skin, in the worst case even in the death of the patient culminating.

Superinfections of the aforementioned kind are z. B. the fullscreen of AIDS common secondary diseases. In itself - at least in small Germ densities - innocuous, but under certain circumstances also pronounced pathogenic germs overgrow the healthy heath flora in this way. at AIDS, however, other body organs are affected by superinfections fen.

Likewise, such superinfections in a variety of dermatological Diseases, eg. Atopic dermatitis, eczema, acne, seborrheic Dermatitis or psoriasis is observed. Also many medical and thera peutic measures, eg. As the radio or chemotherapy of tumors illnesses, caused as a side effect, medically induced Immunosuppression or systemic antibiotic treatment, as well external chemical or physical influences (eg environmental pollution, Smog), promote the appearance of superinfections of the external and internal Organs, in particular the skin and mucous membranes.  

Although it is in some cases readily possible, superinfections with Antibiotics, but most of the time, such substances have the effect Part unpleasant side effects. For example, patients are often against Penicillins are allergic, which is why a corresponding treatment in would forbid such a case.

Furthermore, topically administered antibiotics have the disadvantage that they Relieve skin flora not only from the secondary pathogen, but also in itself physiological skin flora severely impair and natural healing process is slowed down in this way again.

Object of the present invention was the disadvantages of the prior Eliminate technology and substances and preparations, such substances containing, by using them super Infections can be cured, the physiological skin flora no suffers significant losses.

Protozoa are parasitically living unicellular organisms with a clearly delineated nucleus, the reproduce asexually (by two- or fourfold division as well Budding), or sexually (gameto-, gamonto- and autogamy). The Food intake from the environment takes place by permeation and by Pino or phagocytosis. Most protozoa can live next to vegetative, mostly mobile state forms (so-called trophozoites) under unfavorable circumstances also form cysts as permanent forms.

Depending on the type and mode of transport, protozoa are divided into four different groups:

• (a) Mastigophora (flagellate with flagella)
• (b) Sarcodina / Rhizopoda (amoeboid movement pattern Plasmaausstülpungen)
• (c) Sporozoa (meandering or sliding pattern of movement)
• (d) Ciliata / Ciliophora (Bewimping or Flagellation).

Parasitic protozoa are found in subtropical and tropical areas often by stinging and sucking insects, but also dirt and Transmission of infection as well as through the food chain.

Some medically and dermatologically relevant protozoonoses are:
Trichomoniasis (caused by Trichomonas vaginalis), Lamblia (caused by Lamblia intestinalis), visceral as well as cutaneous and mucosal Lei shmaniosis (caused for example by Leishmania donovanii, L. tropica, L. brasiliensis, L. mexicana, L. diffusa or L. pifanoi), trypanosomiasis (caused by various species of Trypanosoma), amoebic dysentery and amebiasis (caused, for example, by various Entamoeba species, Jodamoeba butschlii or Naegleria fowleri), coccidosis (by Isospora belli) and balantidium (caused by Balantidium coli).

Protozoan-induced medical and dermatological Phenomena affect, in some cases significantly, human well-being. There is therefore a considerable need for the persons concerned, this one Conditions to remedy. An object of the present invention was so to find effective principles of action against protozoa.

Parasites are monocellular or multicellular plants or animals that live on (= Ectoparasites) or in (= endoparasites) other creatures at their expense nourish with (= pathogenic parasites) or without (apathogenes Parasites) causing disease. The way of life is either aprophytic or purely parasitic, possibly only as Periodic, temporary or stationary parasite. The development of parasites is bound to one or more different host organisms, the Can be an intermediate or final host.

Medicinal and dermatologically important parasites are for example the Helminths, which in turn are found in Trematodae, Cestodae and Nematodae  subdivide. Helminths affecting human well-being are, for example, schistosomiasis (caused by Schistosoma species), Tapeworm infestation of the intestine and other internal organs (caused by for example, Taenia species and Echinococcus species), Ascariasis (caused by Ascaris lumbricoides), enterobiasis (caused by Enterobium vermicularis), paragonimiasis (caused by Paragonium species), filariasis (caused for example by Wucheria bancrofti) as well as others Nematode infestation (caused for example by Trichuris trichura or Trichinella spiralis).

In addition, there are a variety of parasitic on or in humans and animals living insects or arachnids, the medical and dermato cause logical changes in the host organisms. In this regard for the impairment of human well-being responsible Parasitoses are, for example, Accrodermatitis (caused by Cereal mites, for example Pediculoides ventricosus), scabies (caused by Sarcoptes scabii), fly and / or fly larvae infestation (caused for example, by Glossina, Stomoxys, Tabanus, Chrysops, Lucilia, Chrysomya, Cochliamya, Wohlfartia, Cordylobia or Dermatobia species), Mosquito and / or mosquito larvae (caused for example by Aedes-Culex, Anopheles, Phlebotomus, Culicuides, Sumilium or Haemagoges species), Tick infestation (caused for example by Argas persicus and others Argas species, Ornithodorus erraticus and other Ornithodorus species, Orobius rhi phocephalus, Dermacentor, Haemaphysalis, Amblyomma, Ixodes species), Porocephalose (caused by Porocephalus species), flea infestation (caused by, for example, Pulex irritans, Ctenocephalides canis, Xenopsylla cheopsis, Nosophyllus fasciatus or Sarcopsylla penetrans), lice infestation (causes for example by Phthirius pubis, Pediculosus humanus or Pediculosus captits), bug infestation (caused for example by Cimex lectularius, Cimex hemipterus, Panstrongylus megistus, Rhodnius prolixus, Triatoma dimidata, Triatoma infestans, Triatoma sordida or Triatoma brasiliensis) as well as mites infestation (caused for example by Demodexfolliculorum and others  Demodex species and Dermamyskus species, Glyciphagus domesticus Pyemotes species, Sarcoptes species or Trombicula species).

It is of additional importance that the on or in the human Organism living parasites in turn re-carriers of bacteria Mycota, protozoa and viruses can be health and well-being of the host organism, for example of humans, sustainably impair can. There was therefore a need to be effective against parasitosis to find principles that the medical or dermatological Appearance are able to improve. To satisfy this need was Therefore, another object of the present invention.

In contrast to the prokaryotic and eukaryotic cellular Organisms are viruses [virus = lat. Poison] biological structures, which contribute to Biosynthesis need a host cell. Extracellular viruses (also called "virions") called) consist of a single- or double-stranded nucleic acid sequence (DNA or RNA) and a protein coat (called capsid), optionally surrounded by an additional lipid-containing envelope (Envelope). The totality nucleic acid and capsid are also called nucleocapsid. The Classification of viruses was classed according to clinical criteria, nowadays however, mostly according to their structure, their morphology, but especially after the nucleic acid sequence.

Medically important viral genera are, for example, influenza viruses (Family of the Orthomyxoviridae), Lyssaviren (eg rabies, family of the Rhabdoviruses) enteroviruses (eg hepatitis A, family Picornaviridae), Hepadnaviruses (eg hepatitis B, family Hepadnaviridae).

There are no virucidal substances, in the true sense of the term, that kill viruses. because viruses do not have their own metabolism. It became out of this Basically also discussed whether viruses should be classified as living things. Pharmacological intervention without damaging the unaffected cells  certainly difficult. Possible mechanisms of action in the fight against the viruses are primarily the disorder whose replication, z. B. by blocking the for replication of important enzymes present in the host cell. Furthermore, can the release of viral nucleic acids into the host cell can be prevented. In the context of the hereby presented disclosure is under terms such as "antiviral" or "effective against viruses", "virucidal" or similar property a substance, a single or multicellular organism harmful consequences of a viral infection, be it prophylactic or therapeu regardless of what the actual mechanism of action in a particular case.

The state of the art, however, lacks effective against viruses Substances which also do not represent the host organism or not damage the dimensions.

An object of the present invention was thus to remedy this evil helping to find substances that are effective one or more cellular organism from harmful consequences of a viral infection, be it pro phylactically or therapeutically.

By the active compounds according to the invention and the Zuberei thus obtained The above tasks are also solved. The invention According active ingredients are ideal for the treatment of said Conditions and diseases.

With the active compounds and preparations according to the invention containing them, May attach to the adhesion of all microorganisms, viruses, parasites and protozoa Surfaces are reduced or avoided. This is the case, for example in bacteria, in Gram-positive and Gram-negative bacteria Yeasts, fungi, dermatophytes, viruses, viroids and prions.  

In particular, the following microorganisms, viruses, parasites and protozoa for example, and the disorders and diseases caused by them According to the invention, they can be treated particularly well, especially topically become:

1. Gram-positive bacteria

As examples:
Optionally pathogenic and pathogenic Micrococcaceae, especially Staphylococcus epidermidis, e.g. B. in the development of underarm odor and atopic eczema and Staphylococcus aureus as an important pathogen, z. B. in atopic eczema, atopic dermatitis and psoriasis, Corynebacterium spec., Z. B. in the formation of underarm odor, Propionibacterium spec., Z. As in the development of acne and impure skin.

2. Gram-negative bacteria

As examples:
Escherichia coli, e.g. In ulcerative colitis; Pseudomonas aeruginosa, e.g. B. in superinfections of open wounds, z. B. in the abdomen and cystic fibrosis, Enterococcaceae, z. B. in gastrointestinal infections. The active ingredients can be used during flushing and peroral.

3. Yeasts

As examples:
Pityrosporum ovale, z. B. responsible for dandruff and z. In pityriasis versicolor, pityrosporum folliculitis, seborrheic dermatitis, psoriasis, cutaneous and systemic mycoses, in AIDS and related diseases, Candida albicans, e.g. B. responsible for the development of cutaneous candidiasis. The active ingredients can be administered topically, parenterally, but also perorally. Preferably, the intravascular administration, z. B. as an infusion or injection.

4. mushrooms

As examples:
Mucor spec., E.g. B. responsible for Mucor mycoses and thrush; Aspergillus niger, e.g. B. responsible for cutaneous aspergillosis and Cryptococcus neoformans, z. In cryptococidoidosis. The active ingredients can be administered as described for "yeasts".

5. dermatophytes

As examples:
z. B. responsible for the development of dermatophytoses, z. B. athlete's foot. The active ingredients can be administered as described for "yeasts".

6. Viruses

As examples:
Herpes simplex virus type 1 and 2, varicella zoster virus (shingles); Epstein-Barr virus (Pfeiffer glandular fever), cytomegalovirus, wart viruses and papilloma viruses but also other known viruses. The active ingredients can be administered as described in "yeasts".

Protozoa are parasitically living unicellular organisms with a clearly delineated nucleus, the reproduce asexually (by two- or fourfold division as well Budding), or sexually (gameto-, gamonto- and autogamy). The Food intake from the environment takes place by permeation and by Pino or phagocytosis. Most protozoa can live next to vegetative, mostly mobile state forms (so-called trophozoites) under unfavorable circumstances also form cysts as permanent forms.

Depending on the type and mode of transport, protozoa are divided into four different groups:

• (a) Mastigophora (flagellate with flagella)
• (b) Sarcodina / Rhizopoda (amoeboid movement pattern Plasmaausstülpungen)  
• (c) Sporozoa (meandering or sliding pattern of movement)
• (d) Ciliata / Ciliophora (Bewimping or Flagellation).

Parasitic protozoa are found in subtropical and tropical areas often by stinging and sucking insects, but also dirt and Transmission of infection as well as through the food chain.

Some medically and dermatologically relevant protozoonoses are: Trichomoniasis (caused by Trichomonas vaginalis), Lamblia (caused by Lamblia intestinalis), visceral as well as cutaneous and mucosal lei shamaniosis (caused for example by Leishmania donovanii, L. tropica, Brazilisis, L. mexicana, L. diffusa or L. pifanoi), trypanosomiasis (caused by various Trypanosoma species), amoebic dysentery and amoeba asis (caused, for example, by various Entamoeba species, Jodamoeba butschlii or Naegleria fowleri), coccidosis (by Isospora belli) and Balantidenruhr (caused by Balantidium coli).

Protozoan-induced medical and dermatological Phenomena affect, in some cases significantly, human well-being. There is therefore a considerable need for the persons concerned, this one Conditions to remedy. An object of the present invention was therefore to find effective principles of action against protozoa.

Parasites are monocellular or multicellular plants or animals that live on (= Ectoparasites) or in (= endoparasites) other creatures at their expense nourish with (= pathogenic parasites) or without (apathogenes Parasites) causing disease. The way of life is either aprophytic or purely parasitic, possibly only as Periodic, temporary or stationary parasite. The development of parasites is bound to one or more different host organisms, the Can be an intermediate or final host.  

Medicinal and dermatologically important parasites are for example the Helminths, which in turn are found in Trematodae, Cestodae and Nematodae subdivide. Helminths affecting human well-being are, for example, schistosomiasis (caused by Schistosoma species), Tapeworm infestation of the intestine and other internal organs (caused by for example, Taenia species and Echinococcus species), Ascariasis (caused by Ascaris lumbricoides), enterobiasis (caused by Enterobium vermicularis), paragonimiasis (caused by Paragonium species), filariasis (caused for example by Wucheria bancrofti) as well as others Nematode infestation (caused for example by Trichuris trichura or Trichinella spiralis).

In addition, there are a variety of parasitic on or in humans and animals living insects or arachnids, the medical and dermato cause logical changes in the host organisms. In this regard for the impairment of human well-being responsible Parasitoses are, for example, Accrodermatitis (caused by Cereal mites, for example Pediculoides ventricosus), scabies (caused by Sarcoptes scabii), fly and / or fly larvae infestation (caused for example, by Glossina, Stomoxys, Tabanus, Chrysops, Lucilia, Chrysomya, Cochliamya, Wohlfartia, Cordylobia or Dermatobia species), Mosquito and / or mosquito larvae (caused for example by Aedes-Culex, Anopheles, Phlebotomus, Culicuides, Sumilium or Haemagoges species), Tick infestation (caused for example by Argas persicus and others Argas species, Ornithodorus erraticus and other Ornithodorus species, Orobius rhi phocephalus, Dermacentor, Haemaphysalis, Amblyomma, Ixodes species), Porocephalose (caused by Porocephalus species), flea infestation (caused by, for example, Pulex irritans, Ctenocephalides canis, Xenopsylla cheopsis, Nosophyllus fasciatus or Sarcopsylla penetrans), lice infestation (causes for example by Phthirius pubis, Pediculosus humanus or Pediculosus captits), bug infestation (caused for example by Cimex lectularius, Cimex hemipterus, Panstrongylus megistus, Rhodnius prolixus, Triatoma dimidata,  Triatoma infestans, Triatoma sordida or Triatoma brasiliensis) as well as mites infestation (caused for example by Demodex folliculorum and others Demodex species and Dermamyskus species, Glyciphagus domesticus, Pyemotes species, Sarcoptes species or Trombicula species).

It is of additional importance that the on or in the human Organism living parasites in turn re-carriers of bacteria, Mycota, protozoa and viruses can be health and well-being of the host organism, for example of humans, sustainably impair can. There was therefore a need to be effective against parasitosis To find principles of action that the medical or dermatological Appearance are able to improve. To satisfy this need was Therefore, another object of the present invention.

According to the invention, this object has been achieved. The invention Active ingredients and preparations are for the treatment of parasitosis and Protozoonosen suitable, in particular for the treatment of the above Disorders and diseases.

The use of the active ingredients may be topical, percutaneous, transdermal, parenteral done orally or intravascularly.

Preparations containing active ingredients according to the invention can be topical Preparations, for example, cosmetic and dermatological topical Preparations or else conventional pharmaceutical dosage forms. Deodorants or deodorizing body cleansing are preferred products or personal care products. The active ingredients can also be used in Disinfectants and / or detergents may be included, not only for Treatment of the body or skin are intended, but also to Cleaning and disinfecting hard surfaces, medical materials, Appliances, instruments, furniture and walls.  

For the body certain cleaning agents, disinfectants, detergents can also be used to treat the skin like the skin topical preparations. But they are preferably used for the treatment of Body cavities, wounds and also the mouth and throat as well as the nose.

The active compounds according to the invention can be prepared by conventional pharmaceutical compatible diluents or carriers and optionally with others Auxiliaries are mixed and administered, for example, orally or parenterally become. They may preferably be administered orally in the form of granules, capsules, pills, Tablets, coated tablets, dragees, syrups, emulsions, suspensions, Dispersions, aerosols and solutions and liquids, or as Zäpfen, vaginal balls or parenteral z. In the form of solutions, emulsions or suspensions. Orally administered preparations may contain one or more additives such as sweeteners, flavoring agents, Dyes and preservatives included. Tablets can be the active ingredient containing mixed with conventional pharmaceutically acceptable auxiliaries, for Example of inert diluents such as calcium carbonate, sodium carbonate Lactose and talc, granulating agents and agents that inhibit the disintegration of the tablets when administered orally, such as starch or alginic acid, binders such as Starch or gelatin, lubricants such as magnesium stearate, stearic acid and Talc.

Suitable carriers are, for example, lactose (lactose), gelatin, Corn starch, stearic acid, ethanol, propylene glycol, ethers of tetrahydro furfuryl alcohol and water.

The formulations are prepared, for example, by stretching the Active substances with solvents and / or carriers, optionally under Use of emulsifiers and / or dispersants, wherein z. In the case the use of water as a diluent optionally organic Solvent can be used as auxiliary solvent.  

The application is carried out in a customary manner, preferably orally or parenterally, especially perlingual or intravenous. In the case of oral use can Tablets, of course, in addition to the mentioned carriers also additives, such as sodium citrate, calcium carbonate and dicalcium phosphate together with various additives, such as starch, preferably potato starch, Gelatin and the like. Furthermore, lubricants such as Magnesium stearate, sodium lauryl sulfate and talc for tabletting mitver be used. In the case of aqueous suspensions and / or elixirs, those for oral Applications are thought to have the active ingredients except with the above mentioned excipients with different flavor enhancers or Dyes are added.

In the case of parenteral administration, solutions of the active ingredients may be added Use of suitable liquid carrier materials can be used.

Capsules may contain the active ingredient as a single ingredient or mixed with one solid diluents such as calcium carbonate, calcium phosphate or kaolin contain. The injectable preparations are also known per se Formulated way.

The pharmaceutical preparations may contain the active ingredient in an amount of 0.1 to 90 weight percent, especially 1-90 wt .-%. Be capsules particularly preferred. Single doses contain the active ingredients preferably in one Amount of 0.1 to 10 g.

The cosmetic and / or dermatological according to the invention Formulations may be composed as usual and for treatment the skin and / or the hair in the sense of a dermatological treatment or a treatment in terms of nourishing cosmetics. But you can also be used in make-up products in the decorative cosmetics or in cosmetic and dermatological cleansing products.  

For use, the cosmetic and / or inventive dermatological formulations in the usual for cosmetics and dermatological Applied to the skin and / or hair in sufficient quantity.

Also advantageous are those cosmetic and dermatological preparations, which are in the form of a sunscreen. Advantageously, these contain additionally at least one UVA filter and / or at least one UVB filter and / or at least one inorganic pigment.

The additions of UVA filters and UVB filters or pigments are also available Stabilization of the preparations suitable.

The preparations according to the invention may also preferably comprise substances which absorb UV radiation in the UVB range, the total amount of filter substances z. 0.1% by weight to 30% by weight, preferably 0.5 to 10 wt .-%, in particular 1 to 6 wt .-%, based on the total Weight of the preparation to make cosmetic preparations available put the skin in front of the entire area of ultraviolet radiation protect. They can serve as sunscreen.

The UVB filters may be oil-soluble or water-soluble. As oil-soluble substances z. To name, for example:

• - 3-Benzylidencampher and its derivatives, for. B. 3- (4-methylbenzylidene) - camphor,
• 4-aminobenzoic acid derivatives, preferably 4- (dimethylamino) benzoic acid acid (2-ethylhexyl) ester, 4- (dimethylamino) benzoic acid amyl ester;
• Esters of cinnamic acid, preferably 4-methoxycinnamic acid (2-ethylhexyl) ester, 4-methoxycinnamate;
• Esters of salicylic acid, preferably 2-ethylhexyl salicylate, Salicylic acid (4-isopropylbenzyl) ester, salicylic acid homomenthyl ester;
• - Derivatives of benzophenone, preferably 2-hydroxy-4-meth oxybenzophenone, 2-hydroxy-4-methoxy-4'-methylbenzophenone, 2,2'-dihydroxy-4-methoxybenzophenone;  
• Esters of benzalmalonic acid, preferably 4-methoxybenzalmalonate (2-ethylhexyl) ester;
• 2,4,6-trianilino (p-carbo-2'-ethyl-1'-hexyloxy) -1,3,5-triazine.

As water-soluble substances are advantageous:

• - 2-phenylbenzimidazole-5-sulfonic acid and its salts, eg. For example, sodium, Potassium or triethanolammonium salts,
• Sulfonic acid derivatives of benzophenones, preferably 2-hydroxy-4-meth oxybenzophenone-5-sulfonic acid and its salts;
• - Sulfonic acid derivatives of 3-Benzylidencamphers, such as. B. 4- (2-oxo-3-boron nylidenemethyl) benzenesulfonic acid, 2-methyl-5- (2-oxo-3-bomylidene methyl) sulfonic acid and its salts.

The list of said UVB filters, which find use according to the invention can, of course, not be limiting.

It may also be advantageous in formulations according to the invention UVA filters commonly used in cosmetic and / or dermatological applications preparations are included. Such substances are preferred example derivatives of dibenzoylmethane, in particular 1- (4'-tert-butyl phenyl) -3- (4'-methoxyphenyl) propane-1,3-dione and 1-phenyl-3- (4'-isopropyl phenyl) propan-1,3-dione. Also preparations containing these combinations contain, are the subject of the invention. It can be the same amounts UVA filter substances are used, which for UVB filter substances were called.

Cosmetic and / or dermatological preparations according to the invention may also contain inorganic pigments commonly used in the art Cosmetics are used to protect the skin from UV rays. there are oxides of titanium, zinc, iron, zirconium, silicon, Manganese, aluminum, cerium and mixtures thereof, as well as modifications where the oxides are the active agents. It is particularly preferred  pigments based on titanium dioxide. It can be for the above Combinations mentioned quantities can be used.

Cosmetic and dermatological preparations according to the invention, also z. As to protect the skin from UV rays, can in various forms present as z. B. usually for this type of preparations be used. So they can z. B. a solution, an emulsion of the type Water-in-oil (W / O) or oil-in-water (O / W), or a multiple Emulsions, for example of the water-in-oil-in-water (W / O / W) type, a gel, a hydrodispersion, a solid stick or an aerosol.

The cosmetic preparations according to the invention can be cosmetic Contain excipients, such as those commonly used in such preparations be, for. Preservatives, bactericides, antioxidants, perfumes, agents for preventing foaming, dyes, pigments that have a coloring Have thickening agents, surface-active substances, emulsifiers, softening substances, moisturizing and / or moisturizing substances, Fats, oils, waxes or other common ingredients of a cosmetic Such as alcohols, polyols, polymers, foam stabilizers, electrolytes, organic solvents or silicone derivatives.

If the cosmetic or dermatological preparation is a solution or lotion, the solvent used may be:

• - water or aqueous solutions;
• - Oils, such as triglycerides of capric or caprylic acid, but preferably Castor oil;
• - fats, waxes and other natural and synthetic fats, preferably esters of fatty acids with lower C-number alcohols, e.g. B. with Isopropanol, propylene glycol or glycerol, or esters of fatty alcohols with Alkanoic acids of low C number or with fatty acids;
• Alcohols, diols or polyols of low C number, and their ethers, preferably ethanol, isopropanol, propylene glycol, glycerol, ethylene glycol,  Ethylene glycol monoethyl or monobutyl ether, propylene glycol monomethyl, mono ethyl or monobutyl ether, diethylene glycol monomethyl or mono ethyl ether and analog products.

In particular, mixtures of the abovementioned solvents used. For alcoholic solvents, water can be another Be part of it.

According to the invention as favorable antioxidants all for cosmetic and / or dermatological applications suitable or common Antioxidants are used.

Advantageously, the antioxidants are selected from the group consisting of amino acids (eg glycine, histidine, tyrosine, tryptophan) and their derivatives imidazoles (eg urocanic acid) and their derivatives, peptides such as D, L-carnosine, D-carnosine , L-carnosine and its derivatives (eg anserine), carotenoids, carotenes (eg α-carotene, β-carotene, lycopene) and their derivatives, lipoic acid and its derivatives (eg dihydrolipoic acid), aurothioglucose , Propylthiouracil and other thiols (eg thioredoxin, glutathione, cysteine, cystine, cystamine and their glycosyl, N-acetyl, methyl, ethyl, propyl, amyl, butyl and lauryl, palmitoyl, Oleyl, γ-linoleyl, cholesteryl and glyceryl esters) and their salts, dilauryl thiodipropionate, distearyl thiodipropionate, thiodipropionic acid and derivatives thereof (esters, ethers, peptides, lipids, nucleotides, nucleosides and salts) and sulfoximine compounds (eg buthionine sulfoximines, homocyst insulfoximine, buthione sulfones, penta-, hexa-, heptathiuninsul foximin) in very low tolerated dosages (e.g. Pmol to μmol / kg), furthermore (metal) chelators (for example α-hydroxyfatty acids, palmitic acid, phytic acid, lactoferrin), α-hydroxy acids (for example citric acid, lactic acid, malic acid), humic acid, Bile acid, bile extracts, bilirubin, biliverdin, EDTA, EGTA and their derivatives, unsaturated fatty acids and their derivatives (eg γ-linolenic acid, linoleic acid, oleic acid), folic acid and its derivatives, ubiquinone and ubiquinol and their derivatives, vitamin C and derivatives (eg, ascorbyl palmitate, Mg ascorbyl phosphate, ascorbyl acetate), tocopherols and derivatives (eg, vitamin E acetate), vitamin A and derivatives (vitamin A palmitate), and benzylic resin, rutinic acid and derivatives thereof, ferulic acid, and conyanyl benzoate their derivatives, butylhydroxytoluene, butylhydroxyanisole, nordihydroguaiacetic acid, Nordihy droguajaretsäure, trihydroxybutyrophenone, uric acid and its derivatives mannose and its derivatives, zinc and its derivatives (eg ZnO, ZnSO ₄ ) selenium and its derivatives (eg. Selenium methionine), stilbenes and their derivatives (e.g. B. stilbene oxide, trans-stilbene oxide) and the present invention suitable derivatives (salts, esters, ethers, sugars, nucleotides, nucleosides, peptides and lipids) of these drugs.

The amount of antioxidants (one or more compounds) in the Preparations are preferably from 0.001 to 30% by weight, more preferably 0.05-20 wt .-%, in particular 1-10 wt .-%, based on the total weight the preparation.

If vitamin E and / or its derivatives, the antioxidant (s) is advantageous, their respective concentrations from the range of 0.001-10 wt%, based on the total weight of the formulation choose.

If vitamin A, or vitamin A derivatives, or carotenes or their derivatives the one or more antioxidants are advantageous, their respective Concentrations in the range of 0.001-10 wt .-%, based on the Total weight of the formulation to choose.

Emulsions of the invention are advantageous and contain z. As the mentioned Fats, oils, waxes and other fatty substances, as well as water and an emulsifier, as commonly used for such type of formulation.

Gels according to the invention usually contain lower C-number alcohols, z. As ethanol, isopropanol, 1,2-propanediol, glycerol and water or a above-mentioned oil in the presence of a thickening agent which is oily Alcoholic gels preferably silica or an aluminum silicate, at aqueous-alcoholic or alcoholic gels preferably a polyacrylate.  

Solid pins according to the invention contain z. Natural or synthetic Waxes, fatty alcohols or fatty acid esters. Preference is given to lip balms and deodorant sticks ("deodorant sticks").

As a propellant for inventive sprayable from aerosol containers Cosmetic or dermatological preparations are the usual known volatile, liquefied propellant, z. B. hydrocarbons (propane, Bu tan, isobutane), used alone or in admixture with each other can be. Also, compressed air is advantageous to use.

Of course, the person skilled in the art knows that there are nontoxic propellants per se which basically suitable for the present invention, but nevertheless because of its detrimental effect on the environment or other accompanying circumstances should be waived, especially fluorocarbons and fluorochloro hydrocarbons (CFCs).

The preparations according to the invention may also preferably comprise substances which absorb UV radiation in the UVB range, the total amount of filter substances z. 0.1% by weight to 30% by weight, preferably 0.5 to 10 wt .-%, in particular 1 to 6 wt .-%, based on the total Weight of the preparation to make cosmetic preparations available put the skin in front of the entire area of ultraviolet radiation protect. They can also serve as sunscreen.

Cosmetic preparations according to the present invention may also contain inorganic pigments, which are commonly used in cosmetics for Protecting the skin from UV rays can be used. It refers to Oxides of titanium, zinc, iron, zirconium, silicon, manganese, aluminum, Cers and mixtures thereof, as well as modifications in which the oxides of the active agents are. Particularly preferred are pigments on the Base of titanium dioxide.

Cosmetic preparations for the care of hair are, for example wise to shampoo, preparations, when rinsing the hair before or after shampooing, before or after perming treatment or after the coloring or discoloration of the hair are applied to  Preparations for blow drying or inserting hair, preparations for coloring or discoloring to a barber and treatment lotion, a hair coat or around Permanent wave products.

The cosmetic preparations contain active ingredients and auxiliaries, as they are Usually for this type of preparations for hair care and Haarbe be used.

As auxiliaries serve preservatives, surface-active substances, Substances for preventing foaming, emulsifiers, thickeners, Fats, oils, waxes, organic solvents, bactericides, perfumes, dyes or pigments whose job it is to do the hair or the preparation itself coloring, electrolytes, preparations against the greasing of the hair.

Cosmetic preparations containing a shampoo or a washing, Shower or bath preparation, preferably contain at least an anionic, nonionic or amphoteric surfactant or Mixtures thereof, at least one ethoxylated or propoxylated organic compound in an aqueous medium and adjuvants such as Usually used for this. The surfactant can be used in a concentration between 1 wt .-% and 50 wt .-% in the Shampoo, or the washing, shower or bath preparation, available.

Is the cosmetic or dermatological preparation in the form of a lotion before, the rinsed out and z. B. before or after decolorization, before or after Shampooing, between two shampooing steps, before or after Perming treatment is applied, so it is z. B. order aqueous or aqueous-alcoholic solutions, optionally contain surface-active substances, preferably non-ionic or cationic surfactants whose concentration is between 0.1 and 10% by weight, preferably between 0.2 and 5% by weight. These cosmetic or dermatological preparation can also be an aerosol with the represent commonly used tools.  

A cosmetic preparation in the form of a lotion that is not rinsed out, in particular, a lotion for inserting the hair, a lotion when blown hair is used, a hairdressing and treatment lotion, poses in the generally an aqueous, alcoholic or aqueous-alcoholic solution and contains at least one cationic, anionic, nonionic or amphoteric polymer or mixtures thereof.

Cosmetic and dermatological preparations for the treatment and care of Hair may be in the form of emulsions other than non-ionic or non-ionic are anionic type. Non-ionic emulsions contain oils in addition to water or fatty alcohols which are, for example, polyethoxylated or polypropoxylated can, or mixtures of the two organic components. These Emulsions optionally contain cationic surfactants. Anionic emulsions are preferably of the soap type and are included at least one ethoxylated or propoxylated organic according to the invention Compound of anionic or non-ionic character.

Cosmetic and dermatological preparations for the treatment and care of Hair may be present as gels, in addition to at least one fiction, corresponding alkylated hydroquinone and conventionally used therefor Solvents still organic thickeners, eg. Gum arabic, Xanthan gum, sodium alginate, cellulose derivatives, preferably methylcel lulose, hydroxymethylcellulose, hydroxyethylcellulose, hydroxypropylcellulose, Hydroxypropylmethylcellulose or inorganic thickening agents, e.g. B. Aluminum silicates such as bentonites, or a mixture of Polyethylene glycol and polyethylene glycol stearate or distearate. The Thickener is in the gel z. B. in an amount between 0.1 and 30 wt .-%, preferably between 0.5 and 15% by weight.

Unless otherwise stated, quantities are percentages indications and parts by weight, in particular the total weight of the respective mixture or preparation.

The invention also provides the use of the invention Active ingredients for the preparation of preparations, in particular pharmaceutical Preparations for the treatment of the specified disorders or diseases.

The following examples are intended to illustrate the embodiments of the present invention clarify. The numerical values given are% by weight.

example 1

W / O Cream paraffin oil 10.00 ozokerite 4.00 vaseline 4.00 vegetable oil 10.00 Wool alcohol 2.00 aluminum stearate 0.40 Lipase type 1 0.10 Perfume, preservatives q.s. Water, VES ad 100.00

Example 2

W / O Lotion paraffin oil 25,00 silicone oil 2.00 ceresin 1.50 Wool alcohol 0.50 Glucosesesquiisostearat 2.50 Lipoprotein lipase 0.50 Perfume, preservatives q.s. Water, VES ad 100.00

Example 3

O / W Lotion paraffin oil 5.00 isopropyl palmitate 5.00 cetyl alcohol 2.00 beeswax 2.00 Ceteareth-20 2.00 PEG-20 glyceryl stearate 1.50 glycerin 3.00 Lipase type 2 1.00 Perfume, preservatives q.s. Water, VES ad 100.00

Example 4

O / W cream Vegetable oil 10.00 cetyl alcohol 2.00 glycerol 1.50 PEG-30 glyceryl stearate 2.00 glycerin 3.00 isopropyl palmitate 5.00 Carbopol 980 (neutralized) 0.30 Lipase type XII 0.10 Perfume, preservatives q.s. Water, VES ad 100.00

Example 5

ointment vaseline 36,00 ceresin 10.00 zinc oxide 4.00 Vegetable oil 20.00 Lipase type XI 3.50 Perfume, preservatives q.s. Paraffin oil ad 100.00

Example 6

skin oil cetyl palmitate 3.00 C _12-15 alkyl benzoate 2.00 polyisobutene 10.00 squalane 2.00 Lipoprotein lipase 1.00 Perfume, preservatives q.s. Paraffin oil ad 100.00

Example 7

Bath oil paraffin oil 20.00 PEG-40 hydrogenated castor oil 5.00 Lipase from porcine pancreas 3.00 Perfume, preservatives q.s. Soybean oil ad 100.00

Example 8

lipstick ceresin 8.00 beeswax 4.00 carnauba 2.00 vaseline 40,00 Hydrogenated castor oil 4.00 Lipase A 0.50 Perfume, preservatives q.s. Paraffin oil ad 100.00

Example 9

care mask PEG-50 lanolin 0.50 glyceryl stearate 2.00 Sunflower seed oil 3.00 bentonite 8.00 kaolin 35,00 zinc oxide 5.00 Lipase B 1.00 Perfume, preservatives q.s. Water, VES ad 100.00

Example 10

Liposome-containing gel lecithin 6.00 Vegetable oil 12.50 Hydrolyzed collagen 2.00 Xanthan gum 1.40 butylene 3.00 Rhizomucor lipase 1.00 Perfume, preservatives q.s. Water, VES ad 100.00

Example 11

Shower preparation with moisturizer Cocoamidodiacetat 10.00 sodium lauryl sulfate 25,00 Potassium cocyl hydrolyzed collagen 5.00 macadamia nut oil 5.00 sodium chloride 0.60 Lipase from Candida utilis 2.00 Perfume, preservatives q.s. Water, VES ad 100.00

Example 12

Of soap Na salt from tallow fatty acids 60,00 Na salt from coconut oil 28,00 sodium chloride 0.50 phospholipase 10.00 Perfume, preservatives q.s. Water, VES ad 100.00

Example 13

syndet sodium lauryl sulfate 30.00 sodium sulfosuccinate 10.00 Potassium cocoyl hydrolyzed collagen 2.00 Dimethicone copolyol 2.00 paraffin 2.00 corn starch 10.00 talc 10.00 glycerin 3.00 Lipase type 6-S 5.00 Perfume, preservatives q.s. Water, VES ad 100.00

Example 14

Hair care products TEA-cocoyl hydrolyzed collagen 30.00 monoethanolamine 25,00 almond oil 2.00 sodium chloride 1.00 Phosphatidylinositol phospholipase C 0.20 Perfume, preservatives q.s. Water, VES ad 100.00

Example 15

Care shampoo disodium 6.00 cocoamidopropyl 10.00 glycol distearate 5.00 Lipase type II 0.50 Perfume, preservatives q.s. Water, VES ad 100.00

Example 16

conditioner cetyl alcohol 5.00 Caprylic / Capric Triglycerides 3.00 petrolatum 2.00 Wool alcohol 0.50 Wheat Germ Lipase 1.50 Perfume, preservatives q.s. Water, VES ad 100.00

Example 17

hair conditioner cocoamidopropyl 5.00 cetyl alcohol 2.00 propylene glycol 2.00 citric acid 0.30 Lipase type 7-A 1.00 Perfume, preservatives q.s. Water, VES ad 100.00

Example 18

Haarfestiger Polyvinyl pyrrolidone / vinyl acetate / vinyl propionate copolymer 5.00 ethanol 45,00 Lipiprotein lipase 0.50 Perfume, preservatives q.s. Water, VES ad 100.00

Example 19

Frisiercreme vaseline 4.00 cetearyl 4.00 PEG-40 hydrogenated castor oil 2.00 isopropyl palmitate 5.00 citric acid 1.00 Lipase from Thermus aquaticus 1.00 Perfume, preservatives q.s. Water, VES ad 100.00

Example 20

shaving cream stearic acid 7.00 sodium lauryl sulfate 3.00 stearyl 1.00 glycerin 5.00 triethanolamine 3.60 Lipase from Aspergillus niger 0.10 Perfume, preservatives q.s. Water, VES ad 100.00

Example 21

Foot Cream Soluan 5 2.00 methyl salicylate 5.00 Caprylic / Capric Triglycerides 10.00 stearic acid 5.00 cetyl alcohol 1.00 glycerin 2.00 dimethicone 1.00 Carbopol 984 0.50 triethanolamine 1.50 Lipase from Aspergillus oryzae 2.50 Perfume, preservatives q.s. Water, VES ad 100.00

Example 22

aerosol spray octyldodecanol 0.50 Lipase type 0.1 preservatives q.s. Ethanol ad 100.00

The obtained by mixing the respective ingredients liquid Phase is in proportion with a propane-butane mixture (2: 7) 39: 61 filled in aerosol container.

Example 23

pump spray PEG-40 hydrogenated castor oil 2.00 glycerin 1.00 Lipase type XII 0.1 Perfume, preservatives q.s. Water, VES ad 100.00

Example 24

Roll-on gel 1,3-butylene glycol 2.00 PEG-40 hydrogenated castor oil 2.00 hydroxyethyl 0.50 Lipase type 7-A 0.25 Perfume, preservatives q.s. Water, VES ad 100.00

Example 25

Roll-on emulsion Tricetearethphosphat 0.30 octyldodecanol 2.00 C _12-15 alkyl benzoate 2.00 C _10-30 alkyl acrylate 0.15 Lipoprotein lipase 0.50 Perfume, preservatives q.s. Water, VES ad 100.00

Example 26

Crayon Hydrogenated castor oil 5.00 beeswax 6.00 ceresin 30.00 C _12-15 alkyl benzoate 17.00 Lipase A 2.50 Perfume, preservatives q.s. Octyldodecanol ad 100.00

Example 27 Production of capsules

Capsules containing the ingredients listed below are prepared according to known procedures. These are suitable for the treatment of the above purposes in dosage amounts of one capsule once or several times a day:

Porcine pancreatic lipase 0.10 g or
Lipoprotein lipase 0.10 g.

The numbers in the examples below are parts by weight.

The active compounds according to the invention can be used particularly advantageously in microemulsions. Cosmetic and dermatological preparations according to the invention can be particularly advantageous as

• a) undigested,
• b) classic, z. B. by addition of polyoxamers, Pluronics, carragenans or plant gums thickened,
• c) by addition of A-B-A triblock copolymers (eg PEG-150 distearate, Fa. Akzo Nobel) or alpha, omega-bis-polyethoxylated silanes or silicones) thickened
• d) by addition of star polymers (eg P EG-300 pentaerythrityl tetrastearate or hydrophobically modified tetrakis-polyethoxylated silanes and silicones) thickened
• e) by adding A-B-A-B multiblock copolymers, starburst polymers,  Dendrimers and other supramolecular crosslinkers (eg, Rheodol TWIS 399, KAO, or PEG-120 methyl glucose dioleate) thickened oil-in-water ser- (O / W-), bicontinuous or water-in-oil (W / O) micro emulsions find use.

Example 28

1,3-di- (2-ethylhexyl) -cyclohexane 35,00 Phospholipase D 2.50 sorbitan 10.00 Water (+ citric acid up to pH 5.5) 45,00

Example 29

1,3-di- (2-ethylhexyl) -cyclohexane 33,00 Lipase type 1 2.50 sorbitan 10.00 Water (+ citric acid up to pH 5.5) 45,00 PEG-150 distearate 2.00

Example 30

Steareth-15 4.80 Glycerol monostearate 2.40 Lipoprotein lipase 2.50 cyclomethicone 3.30 cetearyl 1.70 water 85,30

Example 31

Steareth-15 4.80 Glycerol monostearate 2.40 Lipase B 2.50 cyclomethicone 3.30 cetearyl 17.00 water 83.30 PEG-150 distearate 2.00

Claims (7)

1. Pharmaceutical or cosmetic preparations containing one Compound or several compounds from the group of lipases. 2. Use of one or more compounds from the group lipases as antiadhesive agents to microorganisms, viruses, Parasites and protozoa. 3. Use of a compound or several compounds from the group lipases as antiadhesive agents to microorganisms, viruses, Parasites and protozoa as a constituent of preparations. 4. Preparations containing one or more compounds Compounds from the group of lipases with antiadhesive effect Microorganisms, viruses, parasites and protozoa. 5. Preparations and uses according to claims 1-4, characterized characterized in that the lipases of plant origin or animal or are of bacterial origin or derived from fungi. 6. Preparations according to claims 1-5, characterized in that it is are topical preparations. 7. Use of the active ingredients according to claim 1-5 as active ingredients in Deodorants.