DE19512463A1 - Gene and cDNA encoding human cytokine HCC-1 - Google Patents
Gene and cDNA encoding human cytokine HCC-1Info
- Publication number
- DE19512463A1 DE19512463A1 DE1995112463 DE19512463A DE19512463A1 DE 19512463 A1 DE19512463 A1 DE 19512463A1 DE 1995112463 DE1995112463 DE 1995112463 DE 19512463 A DE19512463 A DE 19512463A DE 19512463 A1 DE19512463 A1 DE 19512463A1
- Authority
- DE
- Germany
- Prior art keywords
- hcc
- gene
- construction
- cdna
- expression
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Ceased
Links
- 101000978381 Homo sapiens C-C motif chemokine 14 Proteins 0.000 title claims abstract description 17
- 239000002299 complementary DNA Substances 0.000 title claims abstract description 15
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 14
- 102100023705 C-C motif chemokine 14 Human genes 0.000 claims description 8
- 108020004414 DNA Proteins 0.000 claims description 4
- 210000001185 bone marrow Anatomy 0.000 claims description 4
- 239000012634 fragment Substances 0.000 claims description 3
- 238000009396 hybridization Methods 0.000 claims description 2
- 238000010276 construction Methods 0.000 claims 5
- 238000010353 genetic engineering Methods 0.000 claims 3
- 239000003550 marker Substances 0.000 claims 3
- 206010028980 Neoplasm Diseases 0.000 claims 2
- 238000001415 gene therapy Methods 0.000 claims 2
- 210000001519 tissue Anatomy 0.000 claims 2
- 108020000948 Antisense Oligonucleotides Proteins 0.000 claims 1
- 206010064571 Gene mutation Diseases 0.000 claims 1
- 108020004711 Nucleic Acid Probes Proteins 0.000 claims 1
- 108091034117 Oligonucleotide Proteins 0.000 claims 1
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 claims 1
- 239000000074 antisense oligonucleotide Substances 0.000 claims 1
- 238000012230 antisense oligonucleotides Methods 0.000 claims 1
- 210000004369 blood Anatomy 0.000 claims 1
- 239000008280 blood Substances 0.000 claims 1
- 238000002512 chemotherapy Methods 0.000 claims 1
- 238000009795 derivation Methods 0.000 claims 1
- 201000010099 disease Diseases 0.000 claims 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims 1
- 230000004064 dysfunction Effects 0.000 claims 1
- 210000000987 immune system Anatomy 0.000 claims 1
- 230000002757 inflammatory effect Effects 0.000 claims 1
- 230000004054 inflammatory process Effects 0.000 claims 1
- 210000004185 liver Anatomy 0.000 claims 1
- 108020004999 messenger RNA Proteins 0.000 claims 1
- 239000002853 nucleic acid probe Substances 0.000 claims 1
- 108091033319 polynucleotide Proteins 0.000 claims 1
- 102000040430 polynucleotide Human genes 0.000 claims 1
- 239000002157 polynucleotide Substances 0.000 claims 1
- 238000003793 prenatal diagnosis Methods 0.000 claims 1
- 230000002062 proliferating effect Effects 0.000 claims 1
- 102000004169 proteins and genes Human genes 0.000 claims 1
- 239000000523 sample Substances 0.000 claims 1
- 210000000952 spleen Anatomy 0.000 claims 1
- 239000007858 starting material Substances 0.000 claims 1
- 239000000126 substance Substances 0.000 claims 1
- 210000001541 thymus gland Anatomy 0.000 claims 1
- 239000013598 vector Substances 0.000 claims 1
- 238000000034 method Methods 0.000 description 3
- AIYUHDOJVYHVIT-UHFFFAOYSA-M caesium chloride Chemical compound [Cl-].[Cs+] AIYUHDOJVYHVIT-UHFFFAOYSA-M 0.000 description 2
- 108090000765 processed proteins & peptides Proteins 0.000 description 2
- 108090000695 Cytokines Proteins 0.000 description 1
- 102000004127 Cytokines Human genes 0.000 description 1
- 101000685956 Homo sapiens SAP domain-containing ribonucleoprotein Proteins 0.000 description 1
- 101150062179 II gene Proteins 0.000 description 1
- 108091028043 Nucleic acid sequence Proteins 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 239000000813 peptide hormone Substances 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 108091008146 restriction endonucleases Proteins 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/52—Cytokines; Lymphokines; Interferons
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Gastroenterology & Hepatology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Toxicology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Abstract
The gene and a cDNA mol. coding for human cytokine HCC-1 are disclosed.
Description
Gegenstand der vorliegenden Erfindung ist die cDNA und das Gen kodierend für ein humanes Polypeptid aus der Klasse der Cytokine, das Cytokin HCC-1. Die Erfindung betrifft die cDNA und das Gen für das humane Chemokin HCC-1 sowie die daraus abgeleiteten modifizierten und nicht modifizierten Teilsequenzen sowie verknüpfte Sequenzen und Muteine. Die Erfindung betrifft darüberhinaus die Promotorsequenz des HCC-1-Gens und seine Verwendung in der Expression anderer Gene.The present invention relates to the cDNA and the gene coding for a human Polypeptide from the class of cytokines, the cytokine HCC-1. The invention relates to the cDNA and the gene for the human chemokine HCC-1 and the derived modified and unmodified partial sequences as well as linked sequences and muteins. The invention also relates to the promoter sequence of the HCC-1 gene and its use in the Expression of other genes.
Das humane Cytokin HCC-1 ist ein Peptidhormon mit 74 Aminosäuren Länge. Daraus abgeleitet wurden Primer zur Identifikation der HCC-1 spezifischen cDNA. Die komplette cDNA aus einer humanen cDNA-Bank des Knochenmarkes sowie die komplette Gensequenz aus einer humanen Genbank wurden nach den dem Fachmann bekannten Standardmethoden isoliert, kloniert und nachfolgend sequenziert. Diese Nukleotidsequenzen sind in Abbildung 1 und 2 dargestellt.The human cytokine HCC-1 is a peptide hormone with a length of 74 amino acids. Out of it Primers for identifying the HCC-1-specific cDNA were derived. The complete cDNA from a human cDNA bank of the bone marrow and the complete gene sequence a human gene bank was converted according to the standard methods known to the person skilled in the art isolated, cloned and subsequently sequenced. These nucleotide sequences are shown in Figure 1 and 2 shown.
Die cDNA wird aus humaner Knochenmarks-cDNA-Bank dargestellt. Dazu werden aus der Peptidsequenz abgeleitete Primer im PCR-Verfahren eingesetzt, amplifizierte Fragmente kloniert und nachfolgend sequenziert. Die erhaltene cDNA-Sequenz ist in Abbildung 1 dargestellt.The cDNA is displayed from the human bone marrow cDNA library. To do this, the Peptide sequence derived primers used in the PCR process, amplified fragments cloned and subsequently sequenced. The cDNA sequence obtained is shown in Figure 1 shown.
Das Gen für HCC-1 wurde mit dem Fachmann bekannten Methoden aus humaner Lambda Fix II Genbank isoliert. Dabei wurden aus 1×10⁶ Phagen-Klonen nach Plaque-Hybridisierung mit einem HCC-1-spezifischen cDNA-Teilfragment, Detektion der positiven Klone und zweifachem Nachscreening drei Phagenklone identifiziert. Die Phagen-DNA aller drei Klone wurde über CsCl-Gradienten aufgereinigt, mit Restriktionsendonukleasen geschnitten und das Restriktionsschnittmuster der Phagen-DNA′s als identisch erkannt. Die DNA wurde nach üblichen Methoden sequenziert. Sie enthielt das komplette Gen für HCC-1. Die Gen-Sequenz ist in Abbildung 2 dargestellt. The gene for HCC-1 was determined using methods known to those skilled in the art from human lambda fix II gene bank isolated. Thereby from 1 × 10⁶ phage clones after plaque hybridization with an HCC-1 specific cDNA fragment, detection of the positive clones and double post-screening identified three phage clones. The phage DNA of all three clones was purified over CsCl gradients, cut with restriction endonucleases and that Restriction cut pattern of the phage DNA's recognized as identical. The DNA was after usual methods sequenced. It contained the complete gene for HCC-1. The gene sequence is shown in Figure 2.
Claims (1)
- 1. Zur Konstruktion von Vektoren zur gentechnologischen Herstellung von HCC-1.
- 2. Zur Ableitung von Strukturanaloga, insbesondere Muteinen und deren gentechnologischer Herstellung.
- 3. Zur Konstruktion von Oligonukleotiden als Nukleinsäuresonden für die analytische Bestimmung.
- 4. In Testsystemen zur Kontrolle der Expression dieser Substanz in Blut, Geweben, insbesondere Knochenmark, Milz, Thymus, Leber und anderen biologischen Ausgangsmaterialien.
- 5. Als diagnostischer Marker bei Tumoren, von Funktionsstörungen des Immunsystems und von inflammatorischen Prozessen.
- 6. Zur Kontrolle der Expression in proliferierenden Geweben, insbesondere Knochenmark, zum Beispiel als Verlaufskontrolle der Chemotherapie bei Tumorleiden.
- 7. Zur Konstruktion von Anti-sense-Oligonukleotiden, zum Beispiel im Rahmen einer Gentherapie.
- 8. Als diagnostischer Marker von entzündlichen Systemerkrankungen.
- 9. Als gewerblich genutzter Marker zu Test-und Standardzwecken.
- 10. Zur Herstellung von Konstrukten aus dem HCC-1-Promotor und anderen Gen- bzw. cDNA-Sequenzen.
- 11. Zur Konstruktion von Primern für die pränatalen Diagnostik insbesondere über genomische PCR.
- 12. Zur Konstruktion von Shuttle-Systemen zur Gentherapie bei durch HCC-1-Genmutation geschädigten Patienten.
- 1. For the construction of vectors for the genetic engineering production of HCC-1.
- 2. For the derivation of structural analogs, especially muteins and their genetic engineering production.
- 3. For the construction of oligonucleotides as nucleic acid probes for analytical determination.
- 4. In test systems for controlling the expression of this substance in blood, tissues, in particular bone marrow, spleen, thymus, liver and other biological starting materials.
- 5. As a diagnostic marker for tumors, immune system dysfunction and inflammatory processes.
- 6. To control expression in proliferating tissues, especially bone marrow, for example as a follow-up control of chemotherapy in tumor diseases.
- 7. For the construction of anti-sense oligonucleotides, for example in the context of gene therapy.
- 8. As a diagnostic marker of inflammatory system diseases.
- 9. As a commercial marker for test and standard purposes.
- 10. For the production of constructs from the HCC-1 promoter and other gene or cDNA sequences.
- 11. For the construction of primers for prenatal diagnosis, in particular via genomic PCR.
- 12. For the construction of shuttle systems for gene therapy in patients damaged by HCC-1 gene mutation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE1995112463 DE19512463A1 (en) | 1995-04-03 | 1995-04-03 | Gene and cDNA encoding human cytokine HCC-1 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE1995112463 DE19512463A1 (en) | 1995-04-03 | 1995-04-03 | Gene and cDNA encoding human cytokine HCC-1 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE19512463A1 true DE19512463A1 (en) | 1996-10-10 |
Family
ID=7758667
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE1995112463 Ceased DE19512463A1 (en) | 1995-04-03 | 1995-04-03 | Gene and cDNA encoding human cytokine HCC-1 |
Country Status (1)
| Country | Link |
|---|---|
| DE (1) | DE19512463A1 (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1997041230A3 (en) * | 1996-04-30 | 1997-12-24 | Forssmann Wolf Georg | Cc-type chemokines |
| US6811773B1 (en) | 1993-12-22 | 2004-11-02 | Human Genome Sciences, Inc. | Human monocyte colony inhibitory factor (M-CIF) polypeptides |
-
1995
- 1995-04-03 DE DE1995112463 patent/DE19512463A1/en not_active Ceased
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6811773B1 (en) | 1993-12-22 | 2004-11-02 | Human Genome Sciences, Inc. | Human monocyte colony inhibitory factor (M-CIF) polypeptides |
| US7041460B2 (en) | 1993-12-22 | 2006-05-09 | Human Genome Sciences, Inc. | Antibodies to monocyte-colony inhibitory factor (M-CIF) |
| US7364865B2 (en) | 1993-12-22 | 2008-04-29 | Human Genome Sciences, Inc. | Antibodies to myeloid progenitor inhibitory factor-1 (MPIF-1) |
| US7851596B2 (en) | 1993-12-22 | 2010-12-14 | Human Genome Sciences, Inc. | Myeloid progenitor inhibitory factor-1 (MPIF-1) polypeptides |
| US6180773B1 (en) | 1994-04-30 | 2001-01-30 | Wolf-Georg Forssmann | Tandem cDNAs encoding chemokines CC-1, CC-2, and CC-3 |
| WO1997041230A3 (en) * | 1996-04-30 | 1997-12-24 | Forssmann Wolf Georg | Cc-type chemokines |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 8110 | Request for examination paragraph 44 | ||
| 8125 | Change of the main classification |
Ipc: C12N 15/19 |
|
| 8131 | Rejection |