DE1265453B - Diagnostic agent intended for the detection of blood - Google Patents
Diagnostic agent intended for the detection of bloodInfo
- Publication number
- DE1265453B DE1265453B DEM52734A DEM0052734A DE1265453B DE 1265453 B DE1265453 B DE 1265453B DE M52734 A DEM52734 A DE M52734A DE M0052734 A DEM0052734 A DE M0052734A DE 1265453 B DE1265453 B DE 1265453B
- Authority
- DE
- Germany
- Prior art keywords
- diagnostic agent
- buffer
- hydroperoxide
- blood
- organic peroxide
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
- 239000008280 blood Substances 0.000 title claims description 9
- 210000004369 blood Anatomy 0.000 title claims description 9
- 229940039227 diagnostic agent Drugs 0.000 title claims description 7
- 239000000032 diagnostic agent Substances 0.000 title claims description 7
- 238000001514 detection method Methods 0.000 title claims description 3
- -1 dialdehyde polysaccharide Chemical class 0.000 claims description 14
- 229920001282 polysaccharide Polymers 0.000 claims description 14
- 239000005017 polysaccharide Substances 0.000 claims description 14
- NUIURNJTPRWVAP-UHFFFAOYSA-N 3,3'-Dimethylbenzidine Chemical compound C1=C(N)C(C)=CC(C=2C=C(C)C(N)=CC=2)=C1 NUIURNJTPRWVAP-UHFFFAOYSA-N 0.000 claims description 7
- 239000000872 buffer Substances 0.000 claims description 7
- 229920000159 gelatin Polymers 0.000 claims description 7
- 150000001451 organic peroxides Chemical class 0.000 claims description 7
- 239000008273 gelatin Substances 0.000 claims description 6
- 235000019322 gelatine Nutrition 0.000 claims description 6
- 239000000126 substance Substances 0.000 claims description 6
- FRIBMENBGGCKPD-UHFFFAOYSA-N 3-(2,3-dimethoxyphenyl)prop-2-enal Chemical compound COC1=CC=CC(C=CC=O)=C1OC FRIBMENBGGCKPD-UHFFFAOYSA-N 0.000 claims description 5
- 229920002085 Dialdehyde starch Polymers 0.000 claims description 5
- 108010010803 Gelatin Proteins 0.000 claims description 5
- 235000011852 gelatine desserts Nutrition 0.000 claims description 5
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 claims description 3
- 230000003647 oxidation Effects 0.000 claims description 3
- 238000007254 oxidation reaction Methods 0.000 claims description 3
- XSZYESUNPWGWFQ-UHFFFAOYSA-N 1-(2-hydroperoxypropan-2-yl)-4-methylcyclohexane Chemical compound CC1CCC(C(C)(C)OO)CC1 XSZYESUNPWGWFQ-UHFFFAOYSA-N 0.000 claims description 2
- JGBAASVQPMTVHO-UHFFFAOYSA-N 2,5-dihydroperoxy-2,5-dimethylhexane Chemical compound OOC(C)(C)CCC(C)(C)OO JGBAASVQPMTVHO-UHFFFAOYSA-N 0.000 claims description 2
- 239000002250 absorbent Substances 0.000 claims description 2
- 230000002745 absorbent Effects 0.000 claims description 2
- SPTHWAJJMLCAQF-UHFFFAOYSA-M ctk4f8481 Chemical compound [O-]O.CC(C)C1=CC=CC=C1C(C)C SPTHWAJJMLCAQF-UHFFFAOYSA-M 0.000 claims description 2
- 239000007787 solid Substances 0.000 claims description 2
- RWGFKTVRMDUZSP-UHFFFAOYSA-N cumene Chemical compound CC(C)C1=CC=CC=C1 RWGFKTVRMDUZSP-UHFFFAOYSA-N 0.000 claims 2
- 239000000203 mixture Substances 0.000 description 13
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 12
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 8
- 244000215068 Acacia senegal Species 0.000 description 7
- 229920000084 Gum arabic Polymers 0.000 description 7
- 235000010489 acacia gum Nutrition 0.000 description 7
- 239000000205 acacia gum Substances 0.000 description 7
- 239000000839 emulsion Substances 0.000 description 7
- 239000000834 fixative Substances 0.000 description 7
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 6
- 238000009835 boiling Methods 0.000 description 5
- 239000000463 material Substances 0.000 description 5
- 239000007979 citrate buffer Substances 0.000 description 4
- 239000006185 dispersion Substances 0.000 description 4
- 150000002432 hydroperoxides Chemical class 0.000 description 4
- 239000001509 sodium citrate Substances 0.000 description 4
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 4
- 210000002700 urine Anatomy 0.000 description 4
- 229920000715 Mucilage Polymers 0.000 description 3
- 239000000853 adhesive Substances 0.000 description 3
- 238000005538 encapsulation Methods 0.000 description 3
- 150000004676 glycans Chemical class 0.000 description 3
- 235000018102 proteins Nutrition 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 239000004094 surface-active agent Substances 0.000 description 3
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 2
- 229920000615 alginic acid Polymers 0.000 description 2
- 235000010443 alginic acid Nutrition 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 230000003197 catalytic effect Effects 0.000 description 2
- YCIMNLLNPGFGHC-UHFFFAOYSA-N catechol Chemical compound OC1=CC=CC=C1O YCIMNLLNPGFGHC-UHFFFAOYSA-N 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 238000007598 dipping method Methods 0.000 description 2
- 239000003995 emulsifying agent Substances 0.000 description 2
- LHGVFZTZFXWLCP-UHFFFAOYSA-N guaiacol Chemical compound COC1=CC=CC=C1O LHGVFZTZFXWLCP-UHFFFAOYSA-N 0.000 description 2
- 238000007654 immersion Methods 0.000 description 2
- 239000004615 ingredient Substances 0.000 description 2
- RLSSMJSEOOYNOY-UHFFFAOYSA-N m-cresol Chemical compound CC1=CC=CC(O)=C1 RLSSMJSEOOYNOY-UHFFFAOYSA-N 0.000 description 2
- RNVCVTLRINQCPJ-UHFFFAOYSA-N o-toluidine Chemical compound CC1=CC=CC=C1N RNVCVTLRINQCPJ-UHFFFAOYSA-N 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- IWDCLRJOBJJRNH-UHFFFAOYSA-N p-cresol Chemical compound CC1=CC=C(O)C=C1 IWDCLRJOBJJRNH-UHFFFAOYSA-N 0.000 description 2
- RZXMPPFPUUCRFN-UHFFFAOYSA-N p-toluidine Chemical compound CC1=CC=C(N)C=C1 RZXMPPFPUUCRFN-UHFFFAOYSA-N 0.000 description 2
- WQGWDDDVZFFDIG-UHFFFAOYSA-N pyrogallol Chemical compound OC1=CC=CC(O)=C1O WQGWDDDVZFFDIG-UHFFFAOYSA-N 0.000 description 2
- 235000019333 sodium laurylsulphate Nutrition 0.000 description 2
- PFTAWBLQPZVEMU-DZGCQCFKSA-N (+)-catechin Chemical compound C1([C@H]2OC3=CC(O)=CC(O)=C3C[C@@H]2O)=CC=C(O)C(O)=C1 PFTAWBLQPZVEMU-DZGCQCFKSA-N 0.000 description 1
- GEYOCULIXLDCMW-UHFFFAOYSA-N 1,2-phenylenediamine Chemical compound NC1=CC=CC=C1N GEYOCULIXLDCMW-UHFFFAOYSA-N 0.000 description 1
- PVRZMTHMPKVOBP-UHFFFAOYSA-N 1-n,4-n-dimethylbenzene-1,4-diamine Chemical compound CNC1=CC=C(NC)C=C1 PVRZMTHMPKVOBP-UHFFFAOYSA-N 0.000 description 1
- JRBJSXQPQWSCCF-UHFFFAOYSA-N 3,3'-Dimethoxybenzidine Chemical compound C1=C(N)C(OC)=CC(C=2C=C(OC)C(N)=CC=2)=C1 JRBJSXQPQWSCCF-UHFFFAOYSA-N 0.000 description 1
- CDOUZKKFHVEKRI-UHFFFAOYSA-N 3-bromo-n-[(prop-2-enoylamino)methyl]propanamide Chemical compound BrCCC(=O)NCNC(=O)C=C CDOUZKKFHVEKRI-UHFFFAOYSA-N 0.000 description 1
- QTBSBXVTEAMEQO-UHFFFAOYSA-M Acetate Chemical compound CC([O-])=O QTBSBXVTEAMEQO-UHFFFAOYSA-M 0.000 description 1
- 102000009027 Albumins Human genes 0.000 description 1
- 108010088751 Albumins Proteins 0.000 description 1
- KSFOVUSSGSKXFI-GAQDCDSVSA-N CC1=C/2NC(\C=C3/N=C(/C=C4\N\C(=C/C5=N/C(=C\2)/C(C=C)=C5C)C(C=C)=C4C)C(C)=C3CCC(O)=O)=C1CCC(O)=O Chemical compound CC1=C/2NC(\C=C3/N=C(/C=C4\N\C(=C/C5=N/C(=C\2)/C(C=C)=C5C)C(C=C)=C4C)C(C)=C3CCC(O)=O)=C1CCC(O)=O KSFOVUSSGSKXFI-GAQDCDSVSA-N 0.000 description 1
- 229920002307 Dextran Polymers 0.000 description 1
- FEWJPZIEWOKRBE-JCYAYHJZSA-N Dextrotartaric acid Chemical compound OC(=O)[C@H](O)[C@@H](O)C(O)=O FEWJPZIEWOKRBE-JCYAYHJZSA-N 0.000 description 1
- ZNZYKNKBJPZETN-WELNAUFTSA-N Dialdehyde 11678 Chemical group N1C2=CC=CC=C2C2=C1[C@H](C[C@H](/C(=C/O)C(=O)OC)[C@@H](C=C)C=O)NCC2 ZNZYKNKBJPZETN-WELNAUFTSA-N 0.000 description 1
- 239000001828 Gelatine Substances 0.000 description 1
- 102000001554 Hemoglobins Human genes 0.000 description 1
- 108010054147 Hemoglobins Proteins 0.000 description 1
- 229920001202 Inulin Polymers 0.000 description 1
- 240000007472 Leucaena leucocephala Species 0.000 description 1
- 235000010643 Leucaena leucocephala Nutrition 0.000 description 1
- 240000003183 Manihot esculenta Species 0.000 description 1
- 235000016735 Manihot esculenta subsp esculenta Nutrition 0.000 description 1
- AFBPFSWMIHJQDM-UHFFFAOYSA-N N-methyl-N-phenylamine Natural products CNC1=CC=CC=C1 AFBPFSWMIHJQDM-UHFFFAOYSA-N 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 239000004372 Polyvinyl alcohol Substances 0.000 description 1
- 229920002125 Sokalan® Polymers 0.000 description 1
- 235000021307 Triticum Nutrition 0.000 description 1
- 244000098338 Triticum aestivum Species 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- HFACYLZERDEVSX-UHFFFAOYSA-N benzidine Chemical compound C1=CC(N)=CC=C1C1=CC=C(N)C=C1 HFACYLZERDEVSX-UHFFFAOYSA-N 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 239000005018 casein Substances 0.000 description 1
- BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 description 1
- 235000021240 caseins Nutrition 0.000 description 1
- ADRVNXBAWSRFAJ-UHFFFAOYSA-N catechin Natural products OC1Cc2cc(O)cc(O)c2OC1c3ccc(O)c(O)c3 ADRVNXBAWSRFAJ-UHFFFAOYSA-N 0.000 description 1
- 235000005487 catechin Nutrition 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 235000010980 cellulose Nutrition 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 229950001002 cianidanol Drugs 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 238000004040 coloring Methods 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
- 235000019329 dioctyl sodium sulphosuccinate Nutrition 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 229960001867 guaiacol Drugs 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 229940025294 hemin Drugs 0.000 description 1
- BTIJJDXEELBZFS-QDUVMHSLSA-K hemin Chemical compound CC1=C(CCC(O)=O)C(C=C2C(CCC(O)=O)=C(C)\C(N2[Fe](Cl)N23)=C\4)=N\C1=C/C2=C(C)C(C=C)=C3\C=C/1C(C)=C(C=C)C/4=N\1 BTIJJDXEELBZFS-QDUVMHSLSA-K 0.000 description 1
- GPRLSGONYQIRFK-UHFFFAOYSA-N hydron Chemical compound [H+] GPRLSGONYQIRFK-UHFFFAOYSA-N 0.000 description 1
- 229940029339 inulin Drugs 0.000 description 1
- JYJIGFIDKWBXDU-MNNPPOADSA-N inulin Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)OC[C@]1(OC[C@]2(OC[C@]3(OC[C@]4(OC[C@]5(OC[C@]6(OC[C@]7(OC[C@]8(OC[C@]9(OC[C@]%10(OC[C@]%11(OC[C@]%12(OC[C@]%13(OC[C@]%14(OC[C@]%15(OC[C@]%16(OC[C@]%17(OC[C@]%18(OC[C@]%19(OC[C@]%20(OC[C@]%21(OC[C@]%22(OC[C@]%23(OC[C@]%24(OC[C@]%25(OC[C@]%26(OC[C@]%27(OC[C@]%28(OC[C@]%29(OC[C@]%30(OC[C@]%31(OC[C@]%32(OC[C@]%33(OC[C@]%34(OC[C@]%35(OC[C@]%36(O[C@@H]%37[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O%37)O)[C@H]([C@H](O)[C@@H](CO)O%36)O)[C@H]([C@H](O)[C@@H](CO)O%35)O)[C@H]([C@H](O)[C@@H](CO)O%34)O)[C@H]([C@H](O)[C@@H](CO)O%33)O)[C@H]([C@H](O)[C@@H](CO)O%32)O)[C@H]([C@H](O)[C@@H](CO)O%31)O)[C@H]([C@H](O)[C@@H](CO)O%30)O)[C@H]([C@H](O)[C@@H](CO)O%29)O)[C@H]([C@H](O)[C@@H](CO)O%28)O)[C@H]([C@H](O)[C@@H](CO)O%27)O)[C@H]([C@H](O)[C@@H](CO)O%26)O)[C@H]([C@H](O)[C@@H](CO)O%25)O)[C@H]([C@H](O)[C@@H](CO)O%24)O)[C@H]([C@H](O)[C@@H](CO)O%23)O)[C@H]([C@H](O)[C@@H](CO)O%22)O)[C@H]([C@H](O)[C@@H](CO)O%21)O)[C@H]([C@H](O)[C@@H](CO)O%20)O)[C@H]([C@H](O)[C@@H](CO)O%19)O)[C@H]([C@H](O)[C@@H](CO)O%18)O)[C@H]([C@H](O)[C@@H](CO)O%17)O)[C@H]([C@H](O)[C@@H](CO)O%16)O)[C@H]([C@H](O)[C@@H](CO)O%15)O)[C@H]([C@H](O)[C@@H](CO)O%14)O)[C@H]([C@H](O)[C@@H](CO)O%13)O)[C@H]([C@H](O)[C@@H](CO)O%12)O)[C@H]([C@H](O)[C@@H](CO)O%11)O)[C@H]([C@H](O)[C@@H](CO)O%10)O)[C@H]([C@H](O)[C@@H](CO)O9)O)[C@H]([C@H](O)[C@@H](CO)O8)O)[C@H]([C@H](O)[C@@H](CO)O7)O)[C@H]([C@H](O)[C@@H](CO)O6)O)[C@H]([C@H](O)[C@@H](CO)O5)O)[C@H]([C@H](O)[C@@H](CO)O4)O)[C@H]([C@H](O)[C@@H](CO)O3)O)[C@H]([C@H](O)[C@@H](CO)O2)O)[C@@H](O)[C@H](O)[C@@H](CO)O1 JYJIGFIDKWBXDU-MNNPPOADSA-N 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- TWNIBLMWSKIRAT-VFUOTHLCSA-N levoglucosan Chemical group O[C@@H]1[C@@H](O)[C@H](O)[C@H]2CO[C@@H]1O2 TWNIBLMWSKIRAT-VFUOTHLCSA-N 0.000 description 1
- BHAAPTBBJKJZER-UHFFFAOYSA-N p-anisidine Chemical compound COC1=CC=C(N)C=C1 BHAAPTBBJKJZER-UHFFFAOYSA-N 0.000 description 1
- KHIWWQKSHDUIBK-UHFFFAOYSA-N periodic acid Chemical compound OI(=O)(=O)=O KHIWWQKSHDUIBK-UHFFFAOYSA-N 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- XNGIFLGASWRNHJ-UHFFFAOYSA-L phthalate(2-) Chemical compound [O-]C(=O)C1=CC=CC=C1C([O-])=O XNGIFLGASWRNHJ-UHFFFAOYSA-L 0.000 description 1
- 229920002451 polyvinyl alcohol Polymers 0.000 description 1
- 229920001592 potato starch Polymers 0.000 description 1
- 239000002243 precursor Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 229950003776 protoporphyrin Drugs 0.000 description 1
- 229940079877 pyrogallol Drugs 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 229940095064 tartrate Drugs 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/72—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood pigments, e.g. haemoglobin, bilirubin or other porphyrins; involving occult blood
- G01N33/721—Haemoglobin
- G01N33/725—Haemoglobin using peroxidative activity
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/904—Oxidation - reduction indicators
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Hematology (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Urology & Nephrology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Description
DEUTSCHESGERMAN
PATENTAMTPATENT OFFICE
AUSLEGESCHRIFTEDITORIAL
Int. Cl.: Int. Cl .:
GOInGOIn
Deutsche Kl.: 421-3/54German class: 421-3 / 54
Nummer: 1265 453Number: 1265 453
Aktenzeichen: M 52734IX b/421File number: M 52734IX b / 421
Anmeldetag: 3. Mai 1962 Filing date: May 3, 1962
Auslegetag: 4. April 1968Open date: April 4, 1968
Die Erfindung betrifft ein diagnostisches Mittel zum Nachweis von Blut in Körperflüssigkeiten. Es sind bereits derartige Mittel in trockener, fester Form bekannt, welche ein organisches Peroxyd, einen auf Blut in Gegenwart organischer Peroxyde durch Oxydation unter Farbänderung ansprechenden Indikator und einen Puffer enthalten.The invention relates to a diagnostic means for detecting blood in body fluids. It such agents are already known in dry, solid form which contain an organic peroxide Blood in the presence of organic peroxides by oxidation with a change in color, an appealing indicator and contain a buffer.
Das Mittel gemäß der Erfindung besteht demgegenüber aus einem mit den genannten Stoffen getränkten saugfähigen Träger in Streifen- oder Stäbchenform und enthält als organisches Peroxyd Cumolhydroperoxyd, Diisopropylbenzolhydroperoxyd, p-Menthanhydroperoxyd oder 2,5-Dimethylhexan-2,5-dihydroperoxyd, welches mit Hilfe eines durch ein Dialdehydpolysaccharid fixierten kolloidalen Stoffes eingekapselt ist, sowie einen Puffer, der den pH-Wert der zu untersuchenden Probe im Bereich von 4 bis 7 hält.In contrast, the agent according to the invention consists of one impregnated with the substances mentioned absorbent carrier in the form of strips or sticks and contains cumene hydroperoxide as an organic peroxide, Diisopropylbenzene hydroperoxide, p-menthane hydroperoxide or 2,5-dimethylhexane-2,5-dihydroperoxide, which is encapsulated with the help of a colloidal substance fixed by a dialdehyde polysaccharide as well as a buffer that keeps the pH of the sample to be examined in the range of 4 to 7.
Der kolloidale Stoff ist vorzugsweise Gelatine und das Dialdehydpolysaccharid Dialdehydstärke, während als Indikator am besten o-Tolidin verwendet wird.The colloidal substance is preferably gelatin and the dialdehyde polysaccharide dialdehyde starch, while the best indicator used is o-tolidine.
Die vorliegende Erfindung beruht auf der katalytischen Wirkung der im Blut vorhandenen prosthetischen Gruppen. Diese katalytisch wirksamen Substanzen, die im Hämoglobin identifiziert sind, gehören zu der allgemeinen Klasse von Hämoproteinen, konjugierten Proteinen, von denen alle die gleichen prosthetischen Gruppen Eisenprotoporphyrin oder Hämin besitzen. Diese prosthetische Gruppe besitzt die Fähigkeit, die Übertragung von Sauerstoff aus einer Sauerstoff quelle zu einem Akzeptor, der seinerseits oxydiert wird, zu katalysieren. Wenn der Akzeptor ein Farbstoffvorläufer ist, der farblos ist, bis er oxydiert wird, und in seiner oxydierten Form farbig ist, dann wird das Vorhandensein der katalytischen Aktivität durch Färbung angezeigt.The present invention is based on the catalytic effect of the prosthetic ones present in the blood Groups. These catalytically active substances that are identified in hemoglobin belong to to the general class of hemoproteins, conjugated proteins, all of which are the same prosthetic groups have iron protoporphyrin or hemin. This prosthetic group owns the ability to transfer oxygen from an oxygen source to an acceptor, in turn is oxidized to catalyze. If the acceptor is a dye precursor that is colorless until it is is oxidized, and is colored in its oxidized form, then the presence of the catalytic Activity indicated by coloring.
Die erfindungsgemäß angewandten Hydroperoxyde besitzen, wenn sie mit den anderen bei der Zubereitung der diagnostischen Zusammensetzung erforderlichen Materialien vermischt sind, geringere Stabilität als erwünscht. Erfindungsgemäß wird daher ein neues Einkapselungsmaterial für die organischen Hydroperoxyde aus Proteinen oder Polysacchariden, wie beispielsweise Gelatine, Algin, Carraghenin, Casein, Albumin oder anderen Materialien dieser Art verwendet. Die Proteine oder Polysaccharide werden durch die Behandlung mit einem Dialdehydpolysaccharid gehärtet.The hydroperoxides used according to the invention have when they are used with the others in the preparation the diagnostic composition required materials are mixed, lower stability than he wishes. According to the invention, therefore, a new encapsulation material for the organic hydroperoxides becomes from proteins or polysaccharides, such as gelatine, algin, carraghenin, casein, Albumin or other materials of this type are used. The proteins or polysaccharides are made hardened by treatment with a dialdehyde polysaccharide.
Das organische Hydroperoxyd wird vorteilhaft mit einem Emulgator, wie beispielsweise Gummiarabikumschleim
(acacia mucilage), oder Polyvinylalkohol, Gummiarabikum - Carboxyvinylpolymere vermischt.
Auch ein oberflächenaktives Mittel, wie Dioctylna-Zum Nachweis von Blut bestimmtes
diagnostisches MittelThe organic hydroperoxide is advantageously mixed with an emulsifier such as gum arabic mucilage (acacia mucilage), or polyvinyl alcohol, gum arabic carboxyvinyl polymers. Also a surfactant such as Dioctylna-For the detection of blood
diagnostic agent
Anmelder:Applicant:
Miles Laboratories, Inc.,Miles Laboratories, Inc.,
Elkhart, Ind. (V. St. A.)Elkhart, Ind. (V. St. A.)
Vertreter:Representative:
Dr. K. Th. Hegel, Patentanwalt,Dr. K. Th. Hegel, patent attorney,
2000 Hamburg 50, Große Bergstr. 2232000 Hamburg 50, Große Bergstr. 223
Als Erfinder benannt:
Ernest C. Adams jun.,
Norman R. Novak, Elkhart, Ind. (V. St. A.)Named as inventor:
Ernest C. Adams Jr.,
Norman R. Novak, Elkhart, Ind. (V. St. A.)
Beanspruchte Priorität:Claimed priority:
V. St. v. Amerika vom 4. Mai 1961 (107 646) - -V. St. v. America May 4, 1961 (107 646) - -
triumsulfosuccinat, kann verwendet werden. Die erhaltene Emulsion wird mit Hilfe eines Puffers, beispielsweise eines Gemisches von Natriumeitrat und Zitronensäure, gepuffert. Es kann auch ein Tartrat, Phosphat, Phthalat, Acetat oder ein anderer Puffer verwendet werden. Der bevorzugte Bereich der Wasserstoffionenkonzentration, auf die die Zusammensetzung abgepuffert wird, beträgt von etwa pH 4 bis pH 7. Vorzugsweise wird der Puffer zuerst mit dem sogenannten Umkapselungsmaterial, beispielsweise Gelatine, vor Zugabe zu der Hydroperoxydemulsion vermischt. Dann wird eine wäßrige Dispersion des Dialdehydpolysaccharids als Fixiermittel zugegeben.trium sulfosuccinate, can be used. The emulsion obtained is with the help of a buffer, for example a mixture of sodium citrate and citric acid, buffered. It can also be a tartrate, Phosphate, phthalate, acetate, or another buffer can be used. The preferred range of hydrogen ion concentration, to which the composition is buffered is from about pH 4 to pH 7. Preferably the buffer is first prepared with the so-called encapsulation material, e.g. gelatin Mixing addition to the hydroperoxide emulsion. Then an aqueous dispersion of the dialdehyde polysaccharide is made added as a fixative.
Gründliches Vermischen ist bei jeder Zugabe von außerordentlicher Bedeutung. Die Zusammensetzung wird dann auf Papierstreifen oder runde Papierblätter aufgebracht. Die Streifen oder Blätter werden getrocknet. Dann wird ein Indikator, wie beispielsweise o-Tolidin, etwa durch Eintauchen aufgebracht.Mixing thoroughly is of the utmost importance with every addition. The composition is then applied to strips of paper or round sheets of paper. The strips or leaves are dried. An indicator such as o-tolidine is then applied, for example by immersion.
Das Dialdehydpolysaccharid kann durch die bekannte Oxydation von Polysacchariden mit Perjodsäure hergestellt werden.The dialdehyde polysaccharide can by the known oxidation of polysaccharides with periodic acid getting produced.
Als Rohstoffe können Mais-, Weizen-, Tapioka- oder Kartoffelstärke, Cellulosen, Dextrane, Alginen, Inulin usw. verwendet werden. Vorzugsweise sollen fünfzig bis hundert der ursprünglichen Anhydroglucosegruppen in Dialdehydgruppen übergeführt sein.Corn, wheat, tapioca or potato starch, celluloses, dextrans, algins, Inulin, etc. can be used. Preferably there should be fifty to one hundred of the original anhydroglucose groups be converted into dialdehyde groups.
Bei der vorliegenden Erfindung wird das Dialdehydpolysaccharid in Form einer wäßrigen Dispersion von etwa 0,25 bis etwa 1,5 % verwendet.In the present invention, the dialdehyde polysaccharide is in the form of an aqueous dispersion of about 0.25 to about 1.5% is used.
809 537/280809 537/280
Als Indikatoren können außer o-Tolidin auch o-Toluidin, p-Toluidin, o-Phenylendiamin, N,N'-Dimethyl-p-phenylendiamin, Ν,Ν'-Diäthyl-p-phenylendiamin, Benzidin, p-Anisidin, Dianisidin, o-Krisol, m-Kresol, p-Kresol, «-Naphthol, /^-Naphthol, Catechin, Brenzcatechin, Guajacol und Pyrogallol benutzt werden.In addition to o-tolidine, o-toluidine, p-toluidine, o-phenylenediamine, N, N'-dimethyl-p-phenylenediamine, Ν, Ν'-diethyl-p-phenylenediamine, benzidine, p-anisidine, dianisidine, o-cisol, m-cresol, p-cresol, "-naphthol, / ^ - naphthol, catechin, Catechol, guaiacol and pyrogallol can be used.
Die folgende Tabelle veranschaulicht einige brauchbare Zusammensetzungen:The following table illustrates some useful compositions:
Emulgator (40 bis 60% Gew./Emulsifier (40 to 60% wt /
Vol.) 50 bis 100 mlVol.) 50 to 100 ml
Organisches Hydroperoxyd ... 2,5 bis 10 ml Oberflächenaktives Mittel für dieOrganic hydroperoxide ... 2.5 to 10 ml Surfactant for the
Hydroperoxydemulsion 0 bis 2 mlHydroperoxide emulsion 0 to 2 ml
Puffer I 25 bis 50 mlBuffer I 25 to 50 ml
Einkapselungsmaterial 0,7 bis 3,5 gEncapsulation material 0.7 to 3.5 g
DialdehydpolysaccharidDialdehyde polysaccharide
(1% Gew./Vol.) 0,4 bis 3 ml(1% w / v) 0.4 to 3 ml
Oberflächenaktives Mittel fürSurfactant for
andere Bestandteileother components
(5% Gew./Vol.) 3,75 bis 12 ml(5% w / v) 3.75 to 12 ml
Puffer II (3fache KonzentrationBuffer II (3 times the concentration
von Puffer I) 25 bis 75 mlof buffer I) 25 to 75 ml
Die Herstellung geschieht etwa wie folgt:The production takes place as follows:
In einen Mörser wurden 100 ml Gummiarabikumschleim (hergestellt durch Zugabe von 200 g Gummiarabikum zu 500 ml siedendem Wasser) und anschließend 2 ml Dioctylnatriumsulfosuccinat (5% Gew./Vol.) eingebracht. Dann wurden 5 ml Cumolhydroperoxyd zugegeben, und das Gemisch wurde 5 Minuten unter Bildung einer ersten Emulsion verrieben. Als nächstes wurde eine Citratpufferlösung durch Erhitzen von 1500 ml Wasser zum Sieden und Zugabe zu einem Gemisch von 163 g Natriumeitrat und 37 g Citronensäure hergestellt. Der Citratpuffer wurde dann mit 0,7 g Gelatine vermischt, und 50 ml des Puffer-Gelatine-Gemisches wurden zu der ersten Emulsion zugegeben und mit dieser gut vermischt. Als nächstes wurde 1 ml einer l%igen Dispersion von Dialdehydstärke unter gründlichem Vermischen zugesetzt. Anschließend wurde eine 5%ige Lösung von Natriumlaurylsulfat zu dem Gemisch in einer Menge von 12 ml zugesetzt. Die erhaltene Zusammensetzung wurde dann mit Hilfe eines handbetriebenen Homogenisators homogenisiert, und 50 ml einer, wie oben beschrieben, unter Verwendung von 500 ml Wasser, 163 g Natriumeitrat und 37 g Citronensäure hergestellten Pufferlösung wurde unter gutem Vermischen zugesetzt. Die Endemulsion wurde dann auf Papierstreifen durch Eintauchen derselben aufgebracht, und die imprägnierten Papierstreifen wurden in einem Ofen bei 75 bis 80° C 24 Stunden lang getrocknet. Schließlich wurden 320 mg o-Tolidin in 16 ml Chloroform gelöst und auf die getrockneten Papierstreifen durch Eintauchen derselben aufgebracht. Das überschüssige Chloroform wurde von den Streifen durch mildes einigen erfindungsgemäß unter Verwendung eines Dialdehydpolysaccharids als Fixierungsmittel hergestellten Stäbchen durchgeführt. Die so hergestellten Stäbchen wurden unter verschiedenen Bedingungen bezüglich Temperatur und relativer Feuchtigkeit in jedem Falle 7 Tage lang geprüft. Die Vergleichsergebnisse dieser Prüfungen sind in nachfolgender Tabelle gezeigt, in der A sich auf die Stäbchen, in denen kein Fixierungsmittel verwendet wurde, B auf diejenigen, in denen Formaldehyd als Fixierungsmittel verwendet wurde, und C auf diejenigen, die erfindungsgemäß hergestellt wurden, d. h. auf diejenigen, in denen ein Dialdehydpolysaccharid als Fixierungsmittel verwendet wurde, beziehen.100 ml of gum arabic mucilage (prepared by adding 200 g of gum arabic to 500 ml of boiling water) and then 2 ml of dioctyl sodium sulfosuccinate (5% Weight / volume) introduced. Then 5 ml of cumene hydroperoxide was added and the mixture became Rubbed for 5 minutes to form a first emulsion. Next was a citrate buffer solution by boiling 1500 ml of water and adding to a mixture of 163 g of sodium citrate and 37 g of citric acid. The citrate buffer was then mixed with 0.7 g gelatin, and 50 ml of the buffer-gelatin mixture were added to the first emulsion and mixed well with it. as next was 1 ml of a 1% dispersion of dialdehyde starch added with thorough mixing. A 5% solution of Sodium lauryl sulfate was added to the mixture in an amount of 12 ml. The composition obtained was then homogenized using a hand-operated homogenizer, and 50 ml of one as above described, using 500 ml of water, 163 g of sodium citrate and 37 g of citric acid prepared Buffer solution was added with good mixing. The final emulsion was then put on paper strips applied by dipping them, and the impregnated paper strips were placed in an oven dried at 75 to 80 ° C for 24 hours. Finally, 320 mg of o-tolidine was dissolved in 16 ml of chloroform and applied to the dried paper strips by dipping them. The excess Chloroform was obtained from the strips by mildly according to the invention using a dialdehyde polysaccharide performed as a fixative produced rods. The rods thus produced were made under various conditions tested for temperature and relative humidity in each case for 7 days. The comparison results These tests are shown in the table below, in which A refers to the chopsticks in which no Fixative was used, B to those in which formaldehyde was used as a fixative and C to those made in accordance with the present invention, i.e. H. on those in which one Dialdehyde polysaccharide was used as a fixative.
In einen 1000-ml-Becher wurden 500 ml Gummiarabikumschleim, der durch Zugabe von 500 ml siedendem Wasser zu 200 g Gummiarabikum hergestellt war, und 25 ml Cumolhydroperoxyd eingebracht. Diese Bestandteile wurden in einem mit einem Propellerrührer versehenen Mischer 5 Minuten bei einer Rheostateinstellung auf 70 bis 80 gemischt. In einem anderen Becher wurden 1500 ml siedendes Wasser zu einem Gemisch von 163 g Natriumeitrat und 37 g Citronensäure zugesetzt. Nach Abkühlen wurden 3,5 g Gelatine in dem Citratpuffer gelöst, und das Gemisch wurde in den den Gummiarabikumschleim und das Cumolhydroperoxyd enthaltenden Becher eingebracht. Als nächstes wurden 5 ml einer l%igen (Gew./Vol.) Dispersion von Dialdehydstärke zugegeben. Man ließ die Dialdehydstärke sich mit den anderen Bestandteilen vermischen, gab dann 50 ml Natriumlaurylsulfat zu und setzte das Vermischen 5 Minuten lang bei einer Rheostateinstellung von 60 fort. Die so erhaltene Emulsion wurde durch einen Manton-Gaulin-Homogenisator 5 Minuten lang bei einem Druck von 140 atü500 ml of gum arabic slime prepared by adding 500 ml of boiling water to 200 g of gum arabic and 25 ml of cumene hydroperoxide were placed in a 1000 ml beaker. These ingredients were mixed in a mixer equipped with a propeller stirrer for 5 minutes at a rheostat setting of 70 to 80. In another beaker, 1500 ml of boiling water was added to a mixture of 163 g of sodium citrate and 37 g of citric acid. After cooling, 3.5 g of gelatin was dissolved in the citrate buffer and the mixture was added to the beaker containing the gum arabic slime and cumene hydroperoxide. Next, a l% strength s (wt./vol.) Dispersion of dialdehyde starch was added 5 ml. The dialdehyde starch was allowed to mix with the other ingredients, then 50 ml of sodium lauryl sulfate was added and mixing continued for 5 minutes at a rheostat setting of 60. The emulsion thus obtained was passed through a Manton-Gaulin homogenizer for 5 minutes at a pressure of 140 atm
Erwärmen verdampft. Diese Streifen wurden dann an 60 geführt. Zu dem homogenisierten Gemisch wurdenHeating evaporates. These strips were then passed to 60. To the homogenized mixture were
verschiedenen Urinproben geprüft und zeigten positive Reaktionen bei Proben, die 1 Teil Blut in 30 000 Teilen Urin enthielten.tested various urine samples and showed positive reactions in samples containing 1 part of blood in 30,000 parts Contained urine.
Um die erhöhte Stabilität der erfindungsgemäß hergestellten Diagnosestäbchen zu zeigen, wurde ein Vergleichsversuch zwischen Stäbchen, in denen kein Fixierungsmittel verwendet wurde, einigen, in denen Formaldehyd als Fixierungsmittel verwendet wurde, und 250 ml Citratpuffer, der zuvor durch Zugabe von 500 ml siedendem Wasser zu einem Gemisch von 163 g Natriumeitrat und 37 g Citronensäure hergestellt war, zugegeben, und das Vermischen wurde fortgesetzt. Die Emulsion wurde dann auf Papierstreifen durch Eintauchen derselben aufgebracht, und die imprägnierten Streifen wurden 16 Stunden in einen Ofen bei 71° C gegeben. Schließlich wurden die Streifen in eine Chloro-In order to show the increased stability of the diagnostic sticks produced according to the invention, a comparative experiment was carried out between chopsticks in which no fixative was used, some in which formaldehyde was used as a fixative, and 250 ml of citrate buffer, previously prepared by adding 500 ml of boiling water was made to a mixture of 163 g of sodium citrate and 37 g of citric acid, added and mixing continued. The emulsion was then passed onto strips of paper Immersion was applied and the impregnated strips were placed in an oven at 71 ° C for 16 hours given. Finally the strips were placed in a chloro-
formlösung von o-Tolidin, die durch Auflösen von 1,28 g o-Tolidin in 64 ml Chloroform hergestellt war, eingetaucht, und das überschüssige Chloroform wurde unter Verwendung eines Ofens verdampft. Diese Streifen wurden dann in verschiedenen Urinproben getestet und zeigten sich bei 1 Teil Blut in 25 000 Teilen Urin empfindlich.form solution of o-tolidine, which was prepared by dissolving 1.28 g of o-tolidine in 64 ml of chloroform, immersed, and the excess chloroform was evaporated using an oven. These Strips were then tested in various urine samples and showed 25,000 parts on 1 part of blood Sensitive urine.
Claims (5)
USA.-Patentschrift Nr. 2 799 660.Considered publications:
U.S. Patent No. 2,799,660.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US107646A US3252762A (en) | 1961-05-04 | 1961-05-04 | Stabilized occult blood diagnostic |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE1265453B true DE1265453B (en) | 1968-04-04 |
Family
ID=22317688
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DEM52734A Withdrawn DE1265453B (en) | 1961-05-04 | 1962-05-03 | Diagnostic agent intended for the detection of blood |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US3252762A (en) |
| BE (1) | BE617211A (en) |
| CH (1) | CH427353A (en) |
| DE (1) | DE1265453B (en) |
| DK (1) | DK103213C (en) |
| GB (1) | GB981955A (en) |
| NL (1) | NL277911A (en) |
Families Citing this family (33)
| Publication number | Priority date | Publication date | Assignee | Title |
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| NL126365C (en) * | 1963-06-24 | |||
| DE2235127C2 (en) * | 1972-07-18 | 1974-08-08 | Boehringer Mannheim Gmbh, 6800 Mannheim | Diagnostic agent for the detection of blood and other peroxidatically active substances in body fluids |
| GB1456234A (en) * | 1973-05-24 | 1976-11-24 | Kodak Ltd | Formaldehyde testing material |
| US3975162A (en) * | 1974-03-13 | 1976-08-17 | Marine Colloids, Inc. | Applying reagent to medium and device therefor |
| CS175782B1 (en) * | 1974-10-16 | 1977-05-31 | ||
| JPS5263794A (en) * | 1975-11-21 | 1977-05-26 | Shionogi Seiyaku Kk | Test piece for latent blood |
| US4071318A (en) * | 1977-03-14 | 1978-01-31 | Miles Laboratories, Inc. | Test composition and device for determining peroxidatively active substances |
| US4071321A (en) * | 1977-03-14 | 1978-01-31 | Miles Laboratories, Inc. | Test composition and device for determining peroxidatively active substances |
| US4071317A (en) * | 1977-03-14 | 1978-01-31 | Miles Laboratories, Inc. | Test composition and device for determining peroxidatively active substances and process for preparing same |
| US4175923A (en) * | 1978-06-26 | 1979-11-27 | Friend William G | Method and apparatus for occult blood testing in the home |
| US4526869A (en) * | 1980-09-24 | 1985-07-02 | Regents Of The University Of Minnesota | Method for quantitatively determining the concentration of hemoglobin in a biological sample |
| US4329317A (en) * | 1981-01-29 | 1982-05-11 | Smithkline Instruments, Inc. | Method of stabilizing a specimen slide for occult blood testing |
| US4382064A (en) * | 1981-01-29 | 1983-05-03 | Smithkline Instruments, Inc. | Specimen slide for occult blood testing |
| US4956300A (en) * | 1982-01-05 | 1990-09-11 | Helena Laboratories Corporation | Aid for determining the presence of occult blood, method of making the aid, and method of using the aid |
| DE3311887A1 (en) * | 1983-03-31 | 1984-12-20 | Byk-Mallinckrodt Chemische Produkte Gmbh, 6057 Dietzenbach | METHOD FOR DETERMINING PEROXIDASE ACTIVITY BY FINAL DILUTION TITRATION AND MEANS FOR CARRYING OUT THE METHOD |
| US4447542A (en) * | 1983-04-04 | 1984-05-08 | Miles Laboratories, Inc. | Analytical test composition, device and method for the determination of peroxidatively active substances |
| US4556640A (en) * | 1983-06-29 | 1985-12-03 | Miles Laboratories, Inc. | Stabilized test composition, device and method for the determination of peroxidatively active substances |
| US5702913A (en) * | 1983-12-21 | 1997-12-30 | Helena Laboratories Corporation | Chromgen-reagent test system |
| US5273888A (en) * | 1984-01-16 | 1993-12-28 | Helena Laboratories Corporation | Chemical test kit and method for determining the presence of blood in a specimen and for verifying the effectiveness of the chemicals |
| US4676950A (en) * | 1984-02-03 | 1987-06-30 | Foster Research Corporation | Indicator and test device for detecting occult blood |
| US4615982A (en) * | 1984-12-11 | 1986-10-07 | Lawrence Paul J | Fecal occult blood test |
| NO157437C (en) * | 1985-08-30 | 1988-03-16 | Scandinavian Analytical Produc | TESTING DEVICE FOR DETERMINING BLOOD IN FAECES. |
| US4818702A (en) * | 1986-06-02 | 1989-04-04 | Litmus Concepts, Inc. | Fecal occult blood test reagent |
| US5081040A (en) * | 1987-06-29 | 1992-01-14 | Helena Laboratories Corporation | Composition and kit for testing for occult blood in human and animal excretions, fluids, or tissue matrixes |
| US4895798A (en) * | 1987-11-13 | 1990-01-23 | Miles, Inc. | Test devices for determination of occult blood |
| US5217874A (en) * | 1989-04-04 | 1993-06-08 | Helena Laboratories Corporation | Fecal occult blood test product with positive and negative controls |
| US5196167A (en) * | 1989-04-04 | 1993-03-23 | Helena Laboratories Corporation | Fecal occult blood test product with positive and negative controls |
| DE3925938C1 (en) * | 1989-08-03 | 1990-04-12 | Geb. Sehl Claudia 1000 Berlin De Keck | |
| EP1337830A2 (en) * | 2000-12-01 | 2003-08-27 | Auburn University | Use of acacia gum (arabic gum) to isolate and preserve biological material |
| US7604807B2 (en) * | 2000-12-01 | 2009-10-20 | Auburn University | Use of pullulan to isolate and preserve biological material |
| US6850633B2 (en) * | 2001-02-23 | 2005-02-01 | Beckman Coulter, Inc. | Devices and methods for reading and interpreting guaiac-based occult blood tests |
| GB201107466D0 (en) | 2011-05-05 | 2011-06-15 | Loktionov Alexandre | Device and method for non-invasive collection of colorectal mucocellular layer and disease detection |
| AU2019242190B2 (en) * | 2018-03-27 | 2024-06-13 | Exact Sciences Corporation | Method for stabilizing hemoglobin and reagents for performing the same |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US2799660A (en) * | 1954-04-15 | 1957-07-16 | Miles Lab | Blood test composition and method |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US2290436A (en) * | 1940-09-26 | 1942-07-21 | Miles Lab | Diagnostic composition and method |
| US2754289A (en) * | 1952-02-29 | 1956-07-10 | Shell Dev | Process for polymerizing unsaturated compounds by conducting droplets through an aqueous medium with control of pressure fluctuations |
| NL95045C (en) * | 1953-06-30 | |||
| BE566862A (en) * | 1957-04-23 | |||
| US2986453A (en) * | 1957-11-13 | 1961-05-30 | Miles Lab | Diagnostic compositions |
| US2886445A (en) * | 1958-12-08 | 1959-05-12 | Gen Foods Corp | Process for making chewing gum and product |
| US3043782A (en) * | 1958-12-22 | 1962-07-10 | Upjohn Co | Process for preparing a more impermeable coating by liquid-liquid phase separation |
| NL102893C (en) * | 1959-03-27 | |||
| US3057723A (en) * | 1959-06-24 | 1962-10-09 | Eastman Kodak Co | Hardening of gelatin with oxystarch |
| US3092463A (en) * | 1959-11-02 | 1963-06-04 | Miles Lab | Stable blood detecting composition |
-
0
- NL NL277911D patent/NL277911A/xx unknown
-
1961
- 1961-05-04 US US107646A patent/US3252762A/en not_active Expired - Lifetime
-
1962
- 1962-04-11 GB GB14043/62A patent/GB981955A/en not_active Expired
- 1962-05-02 CH CH524662A patent/CH427353A/en unknown
- 1962-05-03 BE BE617211A patent/BE617211A/en unknown
- 1962-05-03 DK DK200362AA patent/DK103213C/en active
- 1962-05-03 DE DEM52734A patent/DE1265453B/en not_active Withdrawn
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US2799660A (en) * | 1954-04-15 | 1957-07-16 | Miles Lab | Blood test composition and method |
Also Published As
| Publication number | Publication date |
|---|---|
| US3252762A (en) | 1966-05-24 |
| BE617211A (en) | 1962-08-31 |
| NL277911A (en) | |
| CH427353A (en) | 1966-12-31 |
| DK103213C (en) | 1965-11-29 |
| GB981955A (en) | 1965-02-03 |
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