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DE10342794A1 - Sekretion von Proteinen aus Hefen - Google Patents

Sekretion von Proteinen aus Hefen Download PDF

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Publication number
DE10342794A1
DE10342794A1 DE10342794A DE10342794A DE10342794A1 DE 10342794 A1 DE10342794 A1 DE 10342794A1 DE 10342794 A DE10342794 A DE 10342794A DE 10342794 A DE10342794 A DE 10342794A DE 10342794 A1 DE10342794 A1 DE 10342794A1
Authority
DE
Germany
Prior art keywords
shuttle
nucleic acid
proteins
acid sequences
peptides
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
DE10342794A
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English (en)
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
BASF SE
Original Assignee
BASF SE
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by BASF SE filed Critical BASF SE
Priority to DE10342794A priority Critical patent/DE10342794A1/de
Priority to CNA2004800265223A priority patent/CN1852983A/zh
Priority to PCT/EP2004/010346 priority patent/WO2005033316A2/de
Priority to US10/572,189 priority patent/US20070077619A1/en
Priority to EP04765253A priority patent/EP1664306A2/de
Priority to JP2006525798A priority patent/JP2007505602A/ja
Priority to CA002537492A priority patent/CA2537492A1/en
Publication of DE10342794A1 publication Critical patent/DE10342794A1/de
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Mycology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Treatment And Processing Of Natural Fur Or Leather (AREA)
  • Surface Treatment Of Glass (AREA)
  • Treatments For Attaching Organic Compounds To Fibrous Goods (AREA)

Abstract

Die vorliegende Erfindung betrifft Expressionskonstrukte, umfassend die kodierende Nukleinsäuresequenz für ein von Hefezellen prozessierbares Shuttlepeptidkonstrukt; entsprechende Expressionsvektoren, enthaltend derartige Konstrukte; mit deren Hilfe durchgeführte Verfahren zur rekombinanten Herstellung von Zielproteinen; damit transformierte Wirte; Shuttlepeptide und dafür kodierende Nukleinsäuresequenzen; Nukleinsäuresequenzen, kodierend für solche Shuttlepeptide, fusioniert mit einem Fremdprotein; Hydrophobin-Proteine, welche unter Verwendung von derartigen Shuttlepeptiden hergestellt wurden, sowie die Verwendung Hydrophobinen zur Beschichtung von Gegenständen, wie z. B. Leder.
DE10342794A 2003-09-15 2003-09-16 Sekretion von Proteinen aus Hefen Withdrawn DE10342794A1 (de)

Priority Applications (7)

Application Number Priority Date Filing Date Title
DE10342794A DE10342794A1 (de) 2003-09-16 2003-09-16 Sekretion von Proteinen aus Hefen
CNA2004800265223A CN1852983A (zh) 2003-09-16 2004-09-15 蛋白质从酵母的分泌
PCT/EP2004/010346 WO2005033316A2 (de) 2003-09-16 2004-09-15 Sekretion von proteinen aus hefen
US10/572,189 US20070077619A1 (en) 2003-09-15 2004-09-15 Secretion of proteins from yeasts
EP04765253A EP1664306A2 (de) 2003-09-16 2004-09-15 Sekretion von proteinen aus hefen
JP2006525798A JP2007505602A (ja) 2003-09-16 2004-09-15 酵母からのタンパク質の分泌
CA002537492A CA2537492A1 (en) 2003-09-16 2004-09-15 Secretion of proteins from yeasts

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE10342794A DE10342794A1 (de) 2003-09-16 2003-09-16 Sekretion von Proteinen aus Hefen

Publications (1)

Publication Number Publication Date
DE10342794A1 true DE10342794A1 (de) 2005-04-21

Family

ID=34352870

Family Applications (1)

Application Number Title Priority Date Filing Date
DE10342794A Withdrawn DE10342794A1 (de) 2003-09-15 2003-09-16 Sekretion von Proteinen aus Hefen

Country Status (7)

Country Link
US (1) US20070077619A1 (de)
EP (1) EP1664306A2 (de)
JP (1) JP2007505602A (de)
CN (1) CN1852983A (de)
CA (1) CA2537492A1 (de)
DE (1) DE10342794A1 (de)
WO (1) WO2005033316A2 (de)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006131478A3 (de) * 2005-06-06 2007-03-22 Basf Ag Verfahren zur beschichtung von oberflächen von faserigen substraten

Families Citing this family (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
TW200639179A (en) * 2005-02-07 2006-11-16 Basf Ag Method for coating surfaces with hydrophobins
EP1848733B1 (de) * 2005-02-07 2017-06-21 Basf Se Neue hydrophobinfusionsproteine, deren herstellung und verwendung
DE502006007963D1 (de) * 2005-03-30 2010-11-11 Basf Se Verwendung von hydrophobinen zur schmutzabweisenden behandlung von harten oberflächen
MX2007012018A (es) * 2005-03-30 2007-11-16 Basf Ag Uso de hidrofobinas para el tratamiento de superficie de materiales de construccion de mineral endurecido, piedra natural, piedra artificial y ceramica.
MX2007012021A (es) * 2005-03-31 2008-03-14 Basf Ag El uso de polipeptidos como promotores de adhesion.
EP1869138B1 (de) 2005-04-01 2009-11-25 Basf Se Bohrspülung enthaltend hydrophobin
EA012800B1 (ru) * 2005-04-01 2009-12-30 Басф Акциенгезелльшафт Применение белков в качестве деэмульгаторов
DE102005027139A1 (de) * 2005-06-10 2006-12-28 Basf Ag Neue Cystein-verarmte Hydrophobinfusionsproteine, deren Herstellung und Verwendung
DE102005027039A1 (de) * 2005-06-10 2006-12-21 Basf Ag Hydrophobin als Beschichtungsmittel für expandierbare oder expandierte, thermoplastische Polymerpartikel
DE102005029704A1 (de) * 2005-06-24 2007-01-11 Basf Ag Verwendung von Hydrophobin-Polypeptiden sowie Konjugaten aus Hydrophobin-Polypeptiden mit Wirk-oder Effektstoffen und ihre Herstellung sowie deren Einsatz in der Kosmetik
US20080227154A1 (en) * 2005-07-29 2008-09-18 Soren Kjaerulff Protein Expression
JP5105441B2 (ja) * 2005-08-01 2012-12-26 ビーエーエスエフ ソシエタス・ヨーロピア 界面活性非酵素タンパク質の織布洗浄への利用
DE102005048720A1 (de) 2005-10-12 2007-04-19 Basf Ag Verwendung von Proteinen als Antischaum-Komponente in Kraftstoffen
US8096484B2 (en) 2006-08-15 2012-01-17 Basf Se Method for the production of dry free-flowing hydrophobin preparations
AU2008225890B2 (en) * 2007-03-12 2012-03-08 B.R.A.I.N. Biotechnology Research And Information Network Ag Method of treating cellulosic materials with hydrophobins
US20110126143A1 (en) * 2009-11-24 2011-05-26 Williams Michael D System and method for interfacing fitness equipment and physiological monitoring
WO2012004255A1 (de) * 2010-07-07 2012-01-12 Basf Se Zusammensetzung enthaltend ein hydrophobin und verfahren zum reinigen von hydrophoben oberflächen
EP2631296A1 (de) 2012-02-22 2013-08-28 Kimmo Koivu Verfahren zur Hydrophobinherstellung in Pflanzen und Verfahren zur Herstellung von Hydrophobinmultimeren in Pflanzen und Mikroben
EP3942045A1 (de) 2019-03-21 2022-01-26 Onxeo Dbait-molekül in kombination mit einem kinaseinhibitor zur behandlung von krebs
KR20220098759A (ko) 2019-11-08 2022-07-12 인쎄름 (엥스띠뛰 나씨오날 드 라 쌍떼 에 드 라 흐쉐르슈 메디깔) 키나제 억제제에 대해 내성을 획득한 암의 치료 방법
JP7531589B2 (ja) * 2019-12-02 2024-08-09 エルジー・ケム・リミテッド Ｃｈｏ細胞由来のタンパク質分泌因子及びそれを含む発現ベクター
WO2021148581A1 (en) 2020-01-22 2021-07-29 Onxeo Novel dbait molecule and its use
KR102466926B1 (ko) * 2020-05-20 2022-11-14 전남대학교산학협력단 하이드로포빈의 가용성 발현 방법 및 정제 방법
CN115701464B (zh) * 2021-08-02 2024-06-11 江苏金太阳纺织科技股份有限公司 超疏水整理剂及其制备方法与应用

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IT1196484B (it) * 1986-07-11 1988-11-16 Sclavo Spa Vettore ad espressione e secrezione in lieviti,utile per la preparazione di proteine eterologhe
US5010182A (en) * 1987-07-28 1991-04-23 Chiron Corporation DNA constructs containing a Kluyveromyces alpha factor leader sequence for directing secretion of heterologous polypeptides
JP3508157B2 (ja) * 1993-05-19 2004-03-22 旭硝子株式会社 分裂酵母接合フェロモン前駆体遺伝子
GB2281421B (en) * 1993-08-23 1998-04-01 Advanced Risc Mach Ltd Integrated circuit
DE69426289T2 (de) * 1993-12-10 2001-04-12 Korea Institute Of Science And Technology, Seoul/Soul Signalsequenzen für die Secretion heterologer Proteine von Hefe
US5919654A (en) * 1995-02-03 1999-07-06 Asahi Glass Company Ltd. Secretion signal gene and expression vector containing it
AU5914196A (en) * 1995-06-12 1997-01-09 Proefstation Voor De Champignoncultuur Hydrophobins from edible fungi, genes, nucleotide sequences and dna-fragments encoding for said hydrophobins, and expression thereof
JP4341859B2 (ja) * 1996-12-13 2009-10-14 ノバルティス バクシンズ アンド ダイアグノスティックス， インコーポレーテッド 酵母での異種タンパク質の発現の方法
US6130070A (en) * 1997-10-31 2000-10-10 Asahi Glass Company Ltd. Induction promoter gene and secretory signal gene usable in Schizosaccharomyces pombe, expression vectors having the same, and use thereof
AU2001292574A1 (en) * 2000-09-06 2002-03-22 Zymogenetics Inc. Human phermone polypeptide
GB0030038D0 (en) * 2000-12-08 2001-01-24 Univ Warwick Yeast-based assay
FR2833490B1 (fr) * 2001-12-14 2004-12-10 Oreal Utilisition cosmetique d'au moins une hydrophobine pour le traitement des matieres keratiniques et compositions mises en oeuvre

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006131478A3 (de) * 2005-06-06 2007-03-22 Basf Ag Verfahren zur beschichtung von oberflächen von faserigen substraten

Also Published As

Publication number Publication date
CN1852983A (zh) 2006-10-25
JP2007505602A (ja) 2007-03-15
WO2005033316A2 (de) 2005-04-14
CA2537492A1 (en) 2005-04-14
US20070077619A1 (en) 2007-04-05
WO2005033316A3 (de) 2005-10-06
EP1664306A2 (de) 2006-06-07

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