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DE10337407A1 - Method for subcloning nucleic acid fragments, using source vector that contains two sequences recognized by an outside cutter, requires short incubation times and can be adapted to any target vector - Google Patents

Method for subcloning nucleic acid fragments, using source vector that contains two sequences recognized by an outside cutter, requires short incubation times and can be adapted to any target vector Download PDF

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Publication number
DE10337407A1
DE10337407A1 DE2003137407 DE10337407A DE10337407A1 DE 10337407 A1 DE10337407 A1 DE 10337407A1 DE 2003137407 DE2003137407 DE 2003137407 DE 10337407 A DE10337407 A DE 10337407A DE 10337407 A1 DE10337407 A1 DE 10337407A1
Authority
DE
Germany
Prior art keywords
nucleic acid
vector
acid fragments
subcloning
incubation times
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
DE2003137407
Other languages
German (de)
Inventor
Tobias Fromme
Martin Klingenspor
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Philipps-Universitaet Marburg - AG Klebe De
Philipps-Universitaet Marburg 35037 Marburg De
Philipps-Universitaet Marburg De
Original Assignee
Transmit Gesellschaft fuer Technologietransfer mbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Transmit Gesellschaft fuer Technologietransfer mbH filed Critical Transmit Gesellschaft fuer Technologietransfer mbH
Priority to DE2003137407 priority Critical patent/DE10337407A1/en
Publication of DE10337407A1 publication Critical patent/DE10337407A1/en
Withdrawn legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Method for subcloning a nucleic acid fragment (I). Method for subcloning a nucleic acid fragment (I) comprises: (a) providing a source vector (SV) that carries two sequences (X), each containing a recognition site for an outside cutter restriction enzyme (RE1) and boundary nucleotides that, when cut by RE1, form specific overhangs; (b) ligation of (I) into SV between (X); (c) providing a target vector (TV) that has at least one recognition site for a specific restriction enzyme (RE2); and (d) incubating RV and SV in presence of RE1, RE2 and ligase. An independent claim is also included for a kit for subcloning (I) into any TV.
DE2003137407 2003-08-13 2003-08-13 Method for subcloning nucleic acid fragments, using source vector that contains two sequences recognized by an outside cutter, requires short incubation times and can be adapted to any target vector Withdrawn DE10337407A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
DE2003137407 DE10337407A1 (en) 2003-08-13 2003-08-13 Method for subcloning nucleic acid fragments, using source vector that contains two sequences recognized by an outside cutter, requires short incubation times and can be adapted to any target vector

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE2003137407 DE10337407A1 (en) 2003-08-13 2003-08-13 Method for subcloning nucleic acid fragments, using source vector that contains two sequences recognized by an outside cutter, requires short incubation times and can be adapted to any target vector

Publications (1)

Publication Number Publication Date
DE10337407A1 true DE10337407A1 (en) 2005-03-10

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DE2003137407 Withdrawn DE10337407A1 (en) 2003-08-13 2003-08-13 Method for subcloning nucleic acid fragments, using source vector that contains two sequences recognized by an outside cutter, requires short incubation times and can be adapted to any target vector

Country Status (1)

Country Link
DE (1) DE10337407A1 (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008095927A1 (en) * 2007-02-05 2008-08-14 Philipps-Universität Marburg Method of cloning at least one nucleic acid molecule of interest using type iis restriction endonucleases, and corresponding cloning vectors, kits and system using type iis restriction endonucleases
WO2010063711A1 (en) * 2008-12-04 2010-06-10 Istituto Di Ricerche Di Biologia Molecolare P. Angeletti S.P.A. Method for introducing diversity into and assembly of polynucleotide sequences
CN114507904A (en) * 2022-04-19 2022-05-17 北京迅识科技有限公司 Method for preparing second-generation sequencing library

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992001786A1 (en) * 1990-07-26 1992-02-06 Foundation For Research And Technology - Hellas (Fo.R.T.H.) Institute Of Molecular Biology & Biotechnology Portable ribozyme cassettes, dna sequences containing them, ribozymes encoded by these dna sequences, and compositions containing these ribozymes
WO1998033901A2 (en) * 1997-01-31 1998-08-06 Cosmix Molecular Biologicals Gmbh Generation of diversity in combinatorial libraries
WO1998054336A1 (en) * 1997-05-28 1998-12-03 Samyang Genex Corporation Method for mass production of antimicrobial peptide
WO2002070720A1 (en) * 2001-03-02 2002-09-12 Riken Cloning vectors and method for molecular cloning

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992001786A1 (en) * 1990-07-26 1992-02-06 Foundation For Research And Technology - Hellas (Fo.R.T.H.) Institute Of Molecular Biology & Biotechnology Portable ribozyme cassettes, dna sequences containing them, ribozymes encoded by these dna sequences, and compositions containing these ribozymes
WO1998033901A2 (en) * 1997-01-31 1998-08-06 Cosmix Molecular Biologicals Gmbh Generation of diversity in combinatorial libraries
WO1998054336A1 (en) * 1997-05-28 1998-12-03 Samyang Genex Corporation Method for mass production of antimicrobial peptide
WO2002070720A1 (en) * 2001-03-02 2002-09-12 Riken Cloning vectors and method for molecular cloning

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Szybalski,W.,et.al.: Class IIS restriction enzymes - a review. In: Gene, 1991, Vol.100,S.13-26, bes.Fig.5 *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008095927A1 (en) * 2007-02-05 2008-08-14 Philipps-Universität Marburg Method of cloning at least one nucleic acid molecule of interest using type iis restriction endonucleases, and corresponding cloning vectors, kits and system using type iis restriction endonucleases
WO2010063711A1 (en) * 2008-12-04 2010-06-10 Istituto Di Ricerche Di Biologia Molecolare P. Angeletti S.P.A. Method for introducing diversity into and assembly of polynucleotide sequences
CN114507904A (en) * 2022-04-19 2022-05-17 北京迅识科技有限公司 Method for preparing second-generation sequencing library

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Legal Events

Date Code Title Description
OM8 Search report available as to paragraph 43 lit. 1 sentence 1 patent law
8110 Request for examination paragraph 44
8127 New person/name/address of the applicant

Owner name: PHILIPPS-UNIVERSITAET MARBURG, 35037 MARBURG, DE

R081 Change of applicant/patentee

Owner name: PHILIPPS-UNIVERSITAET MARBURG, DE

Free format text: FORMER OWNER: TRANSMIT GESELLSCHAFT FUER TECHNOLOGIETRANSFER MBH, 35394 GIESSEN, DE

Effective date: 20110310

Owner name: PHILIPPS-UNIVERSITAET MARBURG - AG KLEBE, DE

Free format text: FORMER OWNER: TRANSMIT GESELLSCHAFT FUER TECHNOLOGIETRANSFER MBH, 35394 GIESSEN, DE

Effective date: 20110310

R082 Change of representative

Representative=s name: PATENTANWAELTE OLBRICHT, BUCHHOLD, KEULERTZ PARTNE

Representative=s name: PATENTANWAELTE OLBRICHT, BUCHHOLD, KEULERTZ PA, DE

R016 Response to examination communication
R016 Response to examination communication
R016 Response to examination communication
R119 Application deemed withdrawn, or ip right lapsed, due to non-payment of renewal fee