DE10209158A1 - Thin layer chromatography volume-stable standard lipid solution comprises lipid dissolved in solvent pair selected from chlorinated hydrocarbon and alcohol - Google Patents
Thin layer chromatography volume-stable standard lipid solution comprises lipid dissolved in solvent pair selected from chlorinated hydrocarbon and alcoholInfo
- Publication number
- DE10209158A1 DE10209158A1 DE2002109158 DE10209158A DE10209158A1 DE 10209158 A1 DE10209158 A1 DE 10209158A1 DE 2002109158 DE2002109158 DE 2002109158 DE 10209158 A DE10209158 A DE 10209158A DE 10209158 A1 DE10209158 A1 DE 10209158A1
- Authority
- DE
- Germany
- Prior art keywords
- alcohol
- volume
- stable standard
- solvent pair
- standard solution
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
- 150000002632 lipids Chemical class 0.000 claims abstract description 16
- 239000002904 solvent Substances 0.000 claims abstract description 14
- 239000012086 standard solution Substances 0.000 claims abstract description 13
- 238000009835 boiling Methods 0.000 claims abstract description 4
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 3
- 150000008280 chlorinated hydrocarbons Chemical class 0.000 claims abstract description 3
- BDERNNFJNOPAEC-UHFFFAOYSA-N propan-1-ol Chemical compound CCCO BDERNNFJNOPAEC-UHFFFAOYSA-N 0.000 claims description 9
- 229920006395 saturated elastomer Polymers 0.000 claims description 3
- KNKRKFALVUDBJE-UHFFFAOYSA-N 1,2-dichloropropane Chemical compound CC(Cl)CCl KNKRKFALVUDBJE-UHFFFAOYSA-N 0.000 claims 2
- 238000007710 freezing Methods 0.000 abstract description 2
- 230000008014 freezing Effects 0.000 abstract description 2
- 239000000203 mixture Substances 0.000 description 4
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 2
- 238000001704 evaporation Methods 0.000 description 2
- 230000008020 evaporation Effects 0.000 description 2
- 239000003960 organic solvent Substances 0.000 description 2
- KNKRKFALVUDBJE-VMIGTVKRSA-N 1,2-dichloropropane Chemical group [13CH3][13CH](Cl)[13CH2]Cl KNKRKFALVUDBJE-VMIGTVKRSA-N 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 150000004665 fatty acids Chemical class 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 239000011877 solvent mixture Substances 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 239000010409 thin film Substances 0.000 description 1
- 238000004809 thin layer chromatography Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/90—Plate chromatography, e.g. thin layer or paper chromatography
- G01N30/94—Development
Landscapes
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
Description
Die Erfindung betrifft eine volumenstabile Standardlösung für Lipide, die insbesondere für die Dünnschichtchromatographie Verwendung findet. The invention relates to a volume-stable standard solution for lipids that is used in particular for thin layer chromatography.
Lipide haben die Eigenschaft, sich aneinander zu lagern und Aggregate zu bilden. Da sowohl die polaren Kopfgruppen als auch die unpolaren Fettsäuren interagieren können, muss das Lösungsmittel in der Lage sein, beide Funktionen abzuschirmen. Lipids have the property of attaching to each other and aggregates form. Because both the polar head groups and the non-polar Fatty acids can interact, the solvent must be able to shield both functions.
Bisher war es Praxis, Lipide in einem Lösungsmittelsystem aus 2 Teilen Chloroform und 1 Teil Methanol zu lösen und bei -20°C zu lagern (Lottspeich, F., Zorbas, H., Bioanalytik, Kapitel 4 "Lipidanalytik", Spektrum Akademischer Verlag GmbH, Heidelberg 1998, ISBN 3-8274- 0041-4.). So far it has been the practice to use lipids in a solvent system consisting of 2 parts Dissolve chloroform and 1 part methanol and store at -20 ° C (Lottsspeich, F., Zorbas, H., Bioanalytik, Chapter 4 "Lipidanalytik", Spectrum Akademischer Verlag GmbH, Heidelberg 1998, ISBN 3-8274- 0041-4.).
Das Lösungsmittelgemisch besitzt jedoch einen so hohen Dampfdruck, dass es sogar bei tiefer Temperatur durch die Verschlusskappen der Probegläschen diffundiert. Dadurch steigt die Konzentration der Lipide in der Lösung. Um die Vergleichbarkeit der Lösungen zu bewahren ist es aber nötig, die Konzentrationen konstant zu halten. However, the solvent mixture has such a high vapor pressure that even at low temperatures through the caps of the Sample bottles diffused. This increases the concentration of lipids in the solution. It is to keep the comparability of the solutions but necessary to keep the concentrations constant.
Dies trifft besonders auf Lipidstandards zu. Um Lipidklassen zu quantifizieren, muss bislang an jedem Versuchstag eine neue Standardlösung und nachfolgend eine Verdünnungsreihe hergestellt werden. Für eine praktikable Methode ist dies ein zu großer Aufwand. This is particularly true of lipid standards. To lipid classes too to quantify, a new one has Standard solution followed by a series of dilutions become. This is too much effort for a practicable method.
Der Erfindung liegt die Aufgabe zu Grunde, eine volumenstabile Standardlösung für Lipide anzugeben, deren Volumen und Zusammensetzung sich auch bei längerer Lagerung nicht ändert. Gleichzeitig soll ein einfaches Auftragen (Tupfen, "Spotten") der volumenstabilen Standardlösung auf Dünnschichtplatten möglich sein. Weiterhin soll der Gefrierpunkt der volumenstabilen Standardlösung unterhalb von -80°C liegen, weil manche Lipide bei dieser Temperatur gelagert werden und eine Aggregatzustandsänderung ausgeschlossen werden soll. The invention is based on the object, a volume-stable Specify standard solution for lipids, their volume and The composition does not change even after prolonged storage. At the same time, a simple application (dabbing, "spotting") of the volume-stable standard solution on thin-film panels. Furthermore, the freezing point of the volume-stable standard solution should are below -80 ° C because some lipids are at this temperature be stored and a change in physical state excluded shall be.
Das Wesen der Erfindung besteht darin, dass als volumenstabile Standardlösung für Lipide ein Lösungsmittelpaar mit annähernd gleichem Siedepunktlgleichem Dampfdruck bestehend aus einem Chlorkohlenwasserstoff und einem Alkohol verwendet wird. Diese Standardlösung weist stets die selbe Zusammensetzung auf, da immer annähernd gleiche Mengen beider Lösungsmittel verdampfen, wobei der Siedepunkt dieses Lösungsmittelpaars optimaler Weise bei ca. 100°C oder darüber liegt. Damit ist der Dampfdruck gering genug, um bei Lagerung durch niedrige Temperatur (-20°C, -80°C) Verluste durch Verdampfen auszuschließen, und gleichzeitig auch hoch genug, um bei Raumtemperatur ein einfaches Auftragen auf die Dünnschichtplatte zu gewährleisten. The essence of the invention is that as volume stable Standard solution for lipids is a pair of solvents with approximately the same Boiling point vapor pressure consisting of a Chlorinated hydrocarbon and an alcohol is used. This Standard solution always has the same composition, as always evaporate approximately equal amounts of both solvents, the Boiling point of this solvent pair optimally at approx. 100 ° C or above. So the vapor pressure is low enough to Storage through low temperature (-20 ° C, -80 ° C) losses through Exclude evaporation, and at the same time high enough to A simple application to the thin-layer plate at room temperature guarantee.
Ein Beispiel für ein solches Lösemittelsystem ist 1,2-Dichlorpropan/1-Propanol im Verhältnis 2 : 1 (V/W). Bei -20°C bleibt bei dreimonatiger Lagerung das Volumen, die Zusammensetzung und die Konzentration der Lipide konstant. An example of such a solvent system is 1,2-dichloropropane / 1-propanol in a ratio of 2: 1 (V / W). At -20 ° C stays at three months Storage the volume, composition and concentration of the Lipids constant.
Die Aufbewahrung in einer Lösemitteldampf-gesättigten Atmosphäre unterdrückt die Verdunstung von Lipid-Lösungen in organischen Lösemitteln bzw. Gemischen organischer Lösemittel zusätzlich. Eine Lösemitteldampf-gesättigte Atmosphäre kann beispielsweise durch Aufbewahrungsgefäße hergestellt werden, die ein offenes Lösemittel- Reservoire in ihrem Inneren besitzen. Store in a solvent vapor saturated atmosphere suppresses the evaporation of lipid solutions in organic Solvents or mixtures of organic solvents additionally. A Solvent vapor-saturated atmosphere can, for example, by Storage vessels are manufactured that an open solvent Have reservoirs inside.
Claims (4)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE2002109158 DE10209158A1 (en) | 2002-02-26 | 2002-02-26 | Thin layer chromatography volume-stable standard lipid solution comprises lipid dissolved in solvent pair selected from chlorinated hydrocarbon and alcohol |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE2002109158 DE10209158A1 (en) | 2002-02-26 | 2002-02-26 | Thin layer chromatography volume-stable standard lipid solution comprises lipid dissolved in solvent pair selected from chlorinated hydrocarbon and alcohol |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE10209158A1 true DE10209158A1 (en) | 2003-09-11 |
Family
ID=27740589
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE2002109158 Withdrawn DE10209158A1 (en) | 2002-02-26 | 2002-02-26 | Thin layer chromatography volume-stable standard lipid solution comprises lipid dissolved in solvent pair selected from chlorinated hydrocarbon and alcohol |
Country Status (1)
| Country | Link |
|---|---|
| DE (1) | DE10209158A1 (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0263684A2 (en) * | 1986-10-06 | 1988-04-13 | The University of Calgary | Lipid based radiation protection |
| DE3807406A1 (en) * | 1987-04-16 | 1988-10-27 | Fuji Photo Film Co Ltd | METHOD FOR PRODUCING A LIGHT-SENSITIVE LITHOGRAPHIC PLATE |
| DE68920108T2 (en) * | 1988-10-14 | 1995-05-11 | Dow Chemical Co | Process for the preparation of epoxy resins containing aliphatic, non-hydrolyzable chloride. |
| DE19803488A1 (en) * | 1998-01-29 | 1999-08-05 | Nath Guenther | Process for coating plastics based on polyhydrocarbons |
-
2002
- 2002-02-26 DE DE2002109158 patent/DE10209158A1/en not_active Withdrawn
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0263684A2 (en) * | 1986-10-06 | 1988-04-13 | The University of Calgary | Lipid based radiation protection |
| DE3807406A1 (en) * | 1987-04-16 | 1988-10-27 | Fuji Photo Film Co Ltd | METHOD FOR PRODUCING A LIGHT-SENSITIVE LITHOGRAPHIC PLATE |
| DE68920108T2 (en) * | 1988-10-14 | 1995-05-11 | Dow Chemical Co | Process for the preparation of epoxy resins containing aliphatic, non-hydrolyzable chloride. |
| DE19803488A1 (en) * | 1998-01-29 | 1999-08-05 | Nath Guenther | Process for coating plastics based on polyhydrocarbons |
Non-Patent Citations (1)
| Title |
|---|
| FOLCH, J., LEES, M., SOLANE-STANLEY, G.H.: A simple method for the isolation and purification of total lipids from animal tissues. In: J. Biol. Chem. 1957, Bd. 226, S. 497-509 * |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| ON | Later submitted papers | ||
| OP8 | Request for examination as to paragraph 44 patent law | ||
| 8130 | Withdrawal |