DE102018116918A1 - Fluidic detection system - Google Patents

Abstract

The invention relates to a fluidic detection system for the magnetic-electrical measurement of a fluid sample, the system each comprising at least one sample module, a magnetic field sensor and a magnet, which are arranged at a distance from one another. Here, the sample module has at least one channel, which is designed to convey a fluid sample along a flow direction. The magnet generates a magnetic field in a measurement and excitation area of the channel. The detection range of the magnetic field sensor corresponds to the measurement and excitation range of the channel. When the fluid sample is conveyed in the measuring and excitation area of the channel, the magnetic field is modified by the fluid sample to be examined, the magnetic field sensor detecting the modified magnetic field fluid sample.

Description

The invention relates to a fluidic detection system for the magnetic-electrical measurement of a fluid sample.

The next generation of biosensors are facing major challenges due to global demographic change. This concerns in particular the monitoring of the environment, the quality of food as well as the medical diagnosis and pharmaceutical industry. Digital microfluidics (also called drop-based microfluidics), as described, for example, in [1,8], can make a very big contribution to all of these tasks by controlling liquids on a small scale on the milli, micro and nanoscale. Here, two immiscible fluids are specifically sent through a small channel, whereby an emulsion forms at a connection point (for example a T-connection). In the emulsion, a liquid forms a first, continuous phase, which in turn contains a second, dispersed phase in the form of drops or bubbles (see e.g. [8]).

The substances to be examined are fluid, mostly biochemical and liquid samples, which are passed through the channels. The channels for the fluids are usually designed as a fluidic chip, as a capillary, as a tube or as a tube. The fluidic samples to be examined, which are in drop form, can be monitored with a high flow rate in these channels and have a volume of 1 μl to 1 nl. Monitoring is often carried out using optical measurement methods, e.g. using a UV spectrometer or photometer [2,3,6].

Based on the current designs of the detectors, in which, for example, the sample containers run through the coils, the flow rate of the drops through the tubes is currently around 100 to 200 drops per second or 200 µl / min [6,7], of which 30-40 drops per second can be measured [7].

Some companies research and develop in the field of fluidically trained detectors in Europe (e.g. Fluigent, ELVESYS, MilliDrop) and also worldwide (e.g. Biorad, RanDance Technologies). However, most companies develop digital fluidic systems with optical detectors, for example for monitoring bacteria (MilliDrop) or for real-time polymerase chain reaction (Biorad, RanDance Technologies). There are also some commercial activities in the field of sensors for fluidics. For example, MagArray Inc. uses a magnetic detector for molecular diagnosis, but only for static measurements and not in the flow. Other on-chip strategies are aimed at measuring the electrical response, e.g. electrical resistance or impedance. By integrating the sensor elements into the fluidic networks, resistive and capacitive detection in milli, micro and nanofluidics is already well advanced. Nevertheless, especially with resistance measurements, there is a risk of contamination and the problem of reuse due to the contact of the electrodes with the sample. Problems caused by charging can arise when using the impedance of ionic samples. These are significant restrictions that limit the application possibilities of all conventional electronic methods for biological samples. This concerns the monitoring of the reaction kinetics of living microorganisms or the influence of their microenvironment, which fundamentally requires culture media with isotonic buffers. In addition, capacitive detectors require large, expensive impedance analyzers, which counteracts the desired flexibility and compact design.

Disadvantageously, the materials of the channels have to be made transparent by the optical monitoring (e.g. transparent tubes) and the samples have to be provided with dyes in one process step in order to be detected by the optical detectors. In addition, the photodetectors are very expensive, which significantly reduces the cost-effectiveness of the measurements.

Therefore, completely electronically operated detectors are often used, which measure the properties of the materials passed through the channels, such as electrical resistance or impedance, electrically [ 4 . 5 ]. Magnetic-electrical measurements are also possible, in which the tube containing the fluid samples is passed directly through the coil [ 7 ]. Disadvantageously, the coil cannot be made smaller, which means that the detection range cannot be reduced and the measurement of closely spaced drops is not necessary. This is aggravated by the fact that the previous inner and outer diameters of the hoses can no longer be changed.

It is therefore an object to provide a system which overcomes the disadvantages of the prior art when measuring high flow rates and velocities of fluidically formed samples by means of a non-optical measurement method.

In particular, a reliable, inexpensive and time-saving and thus economical measurement should also be carried out for smaller sample volumes and small-sized fluid samples.

This should go hand in hand with a more compact design of the system, so that it is also designed to be portable.

According to the invention, the object is achieved by a fluidic detection system according to the independent claims. Advantageous embodiments of the invention are specified in the dependent claims.

A first aspect of the invention relates to a fluidic detection system for the magnetic-electrical measurement of a fluid sample in micro, milli and nanofluidics, the fluidic detection system comprising different components. According to the invention, the fluidic detection system comprises at least one sample module which has at least one channel which is designed to convey a fluid sample along a flow direction, the longitudinal axis of the channel corresponding to the flow direction. According to the invention, the fluidic detection system further comprises at least one magnet with a magnetic axis, the magnet being designed to generate a magnetic field in a measurement and excitation area of the channel, the dimensions of the magnet being perpendicular to its magnetic axis in at least one dimension is smaller than the outer dimension of the channel perpendicular to its longitudinal axis. According to the invention, the fluidic detection system further comprises at least one magnetic field sensor which is designed to detect a magnetic field and whose detection area includes the measurement and excitation area of the channel of the sample module Detection range of the magnetic field sensor of the dimensioning of the magnet perpendicular to its magnetic axis in at least one dimension.

According to the invention, the fluidic detection system comprises several components. The components of the fluidic detection system according to the invention include at least one magnet, at least one sample module and at least one magnetic field sensor.

In one embodiment, the components of the fluidic detection system according to the invention have variable dimensions and configurations, wherein the components can have different dimensions and configurations from one another. Depending on the intended use, any combinations are advantageously possible. In the following, the dimensioning of a component is also understood to mean the extension or cross-sectional dimension of the respective component.

In embodiments, the fluidics detection system according to the invention is designed to be portable.

According to the invention, the sample module, the magnetic field sensor and the magnet are arranged at a distance from one another. The channel, the magnetic field sensor and the magnet are preferably arranged at a distance from one another. Furthermore, all distances between the components are preferably minimal. In particular, by separating the magnet and the sample module, the configuration and dimensioning of the sample module can advantageously be selected independently of the configuration and dimensioning of the magnet (and vice versa) compared to the prior art [7].

In the sense of the invention, the magnetic axis of the magnet is understood to mean that axis which connects the north pole and south pole of the magnet to one another. The north pole and south pole in turn correspond to the end faces or ends of the magnet, which each have the highest field line density and thus the highest field strength. In one embodiment, the magnetic axis corresponds to the longitudinal axis of the magnet and thus the direction of the greatest extent of the magnet. In a further embodiment, the magnetic axis corresponds to the central axis of the magnet. In a preferred embodiment, the magnetic axis of the at least one magnet intersects the at least one magnetic field sensor.

The magnet and the magnetic field sensor are preferably located exactly opposite one another and thus positioned along the magnetic axis of the magnet. In an alternative embodiment, the at least one magnetic field sensor is not arranged along the magnetic axis of the at least one magnet, but in a different spatial direction and angle of rotation and thus displaced by the magnet and not positioned opposite one another.

In a preferred embodiment, the at least magnet and / or the at least one magnetic field sensor is designed to be adjustable and displaceable. The fluidic detection system can thus advantageously be adapted to the respective geometrical properties of the sample module and the components can also be changed in situ. The at least one magnet is particularly preferably adjustable and displaceable along its magnetic axis.

In embodiments, the adjustment and displacement options of the magnetic field sensor include all possible spatial directions and angles of rotation, in particular changes in distance, displacements with respect to the sample module or azimuthal orientations and changes in the respective angles. Alternatively, the magnet is in all possible spatial directions and angles of rotation designed to be adjustable and displaceable.

According to the invention, the fluidic detection system comprises at least one magnet.

In a preferred embodiment, the at least one magnet is arranged with its magnetic axis perpendicular to the longitudinal axis of the channel of the sample module and thus perpendicular to the direction of flow of the fluid sample flowing through the channel. In a further preferred embodiment, the magnetic axis of the magnet intersects the longitudinal axis of the channel. The at least one magnet is thus centered with respect to the channel, which advantageously increases the effectiveness of the measurement.

In an alternative embodiment, the at least one magnet is not arranged perpendicular to the longitudinal axis of the channel of the sample module along its magnetic axis. However, the measurement signal cannot be fully used in this alternative arrangement. In the sense of the invention, the at least one magnet is designed to generate a magnetic field in a measurement and excitation area of the channel. The magnetic field generated by the at least one magnet is also referred to as the first magnetic field. As a result, if the fluid sample to be examined is present or flows through in the measurement and excitation area, the magnetic field is modified by the fluid sample.

According to the invention, the dimension of the magnet perpendicular to its magnetic axis is smaller in at least one dimension than the outer dimension of the channel perpendicular to its longitudinal axis. "In at least one dimension" refers to a given spatial direction. The outer dimension of the magnet perpendicular to its magnetic axis is preferably smaller in at least one dimension than the outer dimension of the channel perpendicular to its longitudinal axis. This configuration of the dimensioning advantageously enables the number density of the objects, such as the number density of the drops, to be compared to the prior art (see, for example, [ 7 ]) can be increased many times by reducing the distance between two successive objects in the fluid sample due to the smaller measuring and excitation area. The time required to measure the fluid sample is also advantageously reduced.

In a particularly preferred embodiment, the at least one magnet is designed as an electromagnet with at least one coil or as a permanent magnet. By using an electromagnet with at least one coil, the most efficient measurement results are advantageously achieved. In embodiments, if the magnet is designed as an electromagnet with at least one coil, the at least one coil is designed as a hollow cylinder. In a further preferred embodiment, in the case of an electromagnet, the at least one coil is designed as a cylindrical coil. These embodiments advantageously represent the most efficient designs. In alternative embodiments, other designs of the at least one coil of the electromagnet are also possible, for example the at least one coil can be designed as a flat coil.

In one embodiment, the at least one coil of the electromagnet is designed as an air coil. In alternative embodiments, the at least one coil of the electromagnet has a glass core. In a further alternative embodiment, the at least one coil of the electromagnet has an iron core.

In the sense of the invention, if the magnet is designed as an electromagnet with at least one coil, the dimensioning of the magnet perpendicular to its magnetic axis means the coil cross section. In embodiments, when the magnet is designed as an electromagnet with at least one coil, such as, for example, a solenoid, the dimensioning of the magnet corresponds to the diameter of the coil. In a further embodiment, if the channel is designed as a tube, capillary or tube, the coil has a smaller coil cross-section than the outside diameter of the tube, the capillary or the tube. The outer diameter of the hose is 1.6 mm or 1/16 "or 0.8 mm or 1/32".

In embodiments, the at least one magnet is designed to generate a first magnetic field. This first magnetic field is used to excite the fluid sample passed through the channel as soon as the fluid sample being transported comes into the range of the highest field strength of the first magnetic field. The fluid sample to be examined preferably comes into contact with the area of the highest field strength of the first magnetic field in the measurement and excitation area of the channel of the sample module or when passing through it. The range of the highest field strength of the first magnetic field of the magnet preferably covers the measurement and excitation range of the channel of the sample module.

In a preferred embodiment, if the magnet is designed as an electromagnet with at least one coil, the at least one coil is inductive and the first magnetic field of the coil is designed as an alternating field. Depending on the application, the coil is used with high-frequency or low frequency AC operated. In an alternative embodiment, the first magnetic field of the coil is designed as a constant field. Depending on the application, the coil is operated with direct current. In further embodiments, the coil is operated at a frequency in the MHz range at low voltages. The required current depends on the dimensions of the coil and the sensitivity of the magnetic field sensor.

In one embodiment, the first magnetic field serves as a reference for the magnetic field sensor. This is the case if the objects contained in the fluid sample do not modify the first magnetic field.

According to the invention, the fluidic detection system comprises at least one magnetic field sensor as a component. The magnetic field sensor is also referred to as a magnetic field detector, magnetic sensor, magnetometer, magnetic-electrical detector or non-optical detector.

According to the invention, the at least one magnetic field sensor is designed to detect a magnetic field. The magnetic field sensor is sensitive to magnetic fields in its detection area and in the measurement and excitation area of the channel of the fluid module. The at least one magnetic field sensor is preferably designed to detect the modified magnetic field generated by the fluid sample to be examined in the measurement and excitation area. If no magnetic field is modified by the fluid sample to be examined in the measuring and excitation area, the at least one magnetic field sensor is designed to detect the magnetic field generated by the at least one magnet.

In one embodiment, the magnetic field sensor is designed as a SQUID, Hall sensor, field plate or GMR sensor. In further embodiments, the magnetic field sensor is designed as a gradiometer.

The magnetic field sensor can be arranged at any angle and distance around the sample module and thus the channel and / or the magnetic axis of the magnet. However, the detection area of the magnetic field sensor must still be at a sufficient distance from the modified magnetic field forming on the basis of the fluid sample in order to detect a usable signal strength.

The magnetic field sensor can also be a commercially available magnetic field sensor. As a result, no complex electronic circuit is advantageously necessary.

In a preferred embodiment, the at least one magnetic field sensor is designed to detect the strength or the gradient of the magnetic field. In particular, the at least one magnetic field sensor is designed to detect the strength or the gradient of the first magnetic field or the modified magnetic field.

In one embodiment, if the magnetic field sensor is designed as a SQUID, Hall sensor, field plate or GMR sensor, the strength of the first or the modified magnetic field and thus absolute change in the first or the modified magnetic field is detected. In further embodiments, if the magnetic field sensor is designed as a gradiometer, the change in the gradient of the first or of the modified magnetic field is measured. The changes in the first or the modified magnetic field are preferably changes over time.

In one embodiment, the magnetic field sensor is designed to detect the first magnetic field or the modified magnetic field. The magnetic field sensor is preferably arranged along the magnetic axis of the magnet. However, it can also be arranged or shifted in all possible spatial directions and angles of rotation, as long as it is able to detect the first magnetic field or the modified magnetic field.

The number, flow rate and / or speed of the objects contained in the fluid sample can be determined from the modified magnetic field detected by the magnetic field sensor, in particular the temporal change in the signal. The number density of the objects contained in the fluid sample can also be determined from the modified magnetic field. Furthermore, from the relative change in the field strength and the shape of the modified magnetic field, the magnetic, metallic and / or ionic properties of the objects contained in the fluid sample and conveyed through the channel can be determined.

According to the invention, the detection range of the at least one magnetic field sensor corresponds to the dimensioning of the magnet perpendicular to its magnetic axis in at least one dimension. The detection range of the at least one magnetic field sensor preferably corresponds at most to the dimensioning of the magnet perpendicular to its magnetic axis. In the case of an electromagnet with at least one coil, the detection range of the at least one magnetic field sensor corresponds at most to the coil cross section. The detection range of the at least one magnetic field sensor is thus optimally covered by the first magnetic field or the modified magnetic field, which advantageously increases the measurement sensitivity.

Objects of the fluid sample to be examined, which are in the detection area of the magnetic field sensor and are conveyed or flow through it, are therefore located in the measurement and excitation area of the channel of the fluidic detection system according to the invention.

In one embodiment, the measurement and excitation area corresponds to that area of the channel of the sample module which is in the area of the highest field strength of the first magnetic field of the magnet and the detection area of the magnetic field sensor or is covered by this. The measuring and excitation area is always local and is also referred to as the measuring section.

The detection area of the at least one magnetic field sensor preferably corresponds to that area of the sample module or of the channel which is arranged between the magnet and the magnetic field sensor, wherein in this preferred embodiment the magnet and magnetic field sensor are positioned exactly opposite one another.

In an alternative embodiment, the detection area of the at least one magnetic field sensor is in the measurement and excitation area of the channel of the sample module, which is covered by the area of the highest field strength of the first magnetic field or the modified magnetic field in order to generate a measurable signal.

In embodiments, the detection area of the at least one magnetic field sensor is made smaller than the dimensioning of the objects (for example the length of the drops) along the longitudinal axis of the channel. As a result, the distance between two successive objects in the fluid sample can be reduced, which, with respect to the objects contained in the fluid sample, advantageously leads to a multiple increase in the number density of the objects (for example an increased number density of the drops) and thus to a higher flow rate of the objects leads in the fluid sample. This in turn advantageously reduces the time required to measure the fluid sample.

In the sense of the invention, the detection range of the at least one magnetic field sensor includes the measurement and excitation range of the channel of the sample module. The entire magnetic field sensor is thus arranged in the immediate vicinity of the sample module. The magnetic field sensor and in particular its detection area must be arranged or designed such that the magnetic field sensor is still able to detect the first magnetic field or the modified magnetic field.

According to the invention, the fluidic detection system comprises at least one sample module as a component. The sample module corresponds to a sample container. In one embodiment, the sample module is designed as a fluid module.

In further embodiments, the sample module is designed as a fluidic chip. The fluidic chip comprises micro, milli and nanofluidic structures or channels and can therefore be used in micro, milli and nanofluidics. In one embodiment, the sample module with the at least one channel is printed as a fluidic chip, 3D printed, lithographically produced or milled.

In the sense of the invention, the sample module has at least one channel. The channel corresponds to a channel-like conveyor.

According to the invention, the channel is designed to convey a fluid sample along a flow direction. Since it is a fluid sample, the term “fluid sample passed through the channel” is also used below. The fluid sample flows through the at least one channel of the sample module. In embodiments, at least one syringe is connected to the at least one channel, which in turn is connected to at least one pump, the pump adjusting or metering the flow rate of the fluid sample by building up an internal pressure.

According to the invention, the longitudinal axis of the channel corresponds to the direction of flow of the fluid sample. The direction of flow of the fluid sample is also referred to as the direction of conveyance.

In a preferred embodiment, the at least one channel of the at least one sample module is designed as a tube, capillary, tube or as at least one structure on a fluidic chip. In further embodiments, the channel is a microchannel, i.e. with an internal dimensioning of significantly less than 1 mm.

In one embodiment, the channel has an internal dimensioning and an external dimensioning.

In embodiments, the cross section of the internal dimensioning of the channel and / or the cross section of the external dimensioning of the channel has different geometric shapes and dimensions and can be, for example, rectangular or circular. Furthermore, the internal dimensioning and the external dimensioning can have dimensions that can be changed in the flow direction within the course of the channel. The dimensioning of the magnet is then preferred perpendicular to its magnetic axis in at least one dimension smaller than the external dimensioning of the channel in at least one dimension perpendicular to its longitudinal axis.

In the case of a circular cross section, the inside dimension of the channel corresponds to an inside diameter and the outside dimension of the channel corresponds to an outside diameter.

The internal dimensioning of the channel corresponds to a cavity. In one embodiment, if the channel is designed as a tube, capillary or tube, the inside dimension corresponds to the inside diameter of the tube, the capillary or the tube. In alternative embodiments, the inside diameter corresponds to the dimensioning of the channel.

The external dimensioning of the channel corresponds to the required and required dimensions of the sample module. In one embodiment, if the channel is designed as a tube, capillary or tube, the outer dimension corresponds to the outer diameter of the tube, the capillary or the tube. In a further embodiment, if the channel is designed as a structure on a fluidic chip, the external dimensioning of the channel corresponds to the support structure of the fluidic chip.

In embodiments, the inner dimensioning and / or the outer dimensioning of the channel can be flexibly determined by the user, the magnetic field strength of the magnet and the sensitivity of the magnetic field sensor having to be adapted depending on the dimensioning selected.

Since at least one channel is available per sample module, the inside and / or outside dimensioning of which is variable, depending on the application, it is advantageously possible to choose from the required outside and / or inside dimensioning of the channel and to use and vary this in the fluidic detection system according to the invention become.

For example, in the case of a channel designed as a tube, capillary or tube, the outside diameter can range from 1/32 "or 1/16" and the inside diameter from 25 µm to 500 µm. In the case of a channel designed as at least one structure on a fluidic chip, the internal dimensions, which are designed as notches of the structures, and the external dimensions can be designed variably. The flexibility of the fluidic detection system according to the invention is thus advantageously increased by the flow of objects of different dimensions, for example droplets with variable sizes, in the fluid sample, since there are more possible uses and, for example, different industrial or laboratory standards can be implemented. The sample volume can also advantageously be reduced by one thousandth of the original sample volume.

In one embodiment, the sample module has a sample inlet through which the fluid sample is introduced into the sample module. In further embodiments, objects such as drops are already present in the transport medium of the fluid sample. From the sample inlet, the fluid sample to be examined is passed through the at least one channel of the sample module in the flow direction to and through the measurement and excitation area. After the fluid sample to be examined has passed the measurement and excitation area of the channel, it leaves the sample module through a sample exit.

In a further embodiment, the sample module has two sample inlets, through which the fluid sample with the transport medium is introduced into the sample module. A connection point of the sample module, which is designed as a T-connection or crossing point, connects the two sample inlets, through which the objects are formed. If, for example, the object medium of the fluid sample is in the form of a liquid such as water and the transport medium of the fluid sample is in the form of oil, the liquid in the oil is drawn in by viscous shear forces, which increases the contact area and, due to the surface tension of the liquid, which cannot be expanded at will, objects, in the present case drops, are formed. From the T-connection or the crossing point, the objects designed as drops are directed in the flow direction of the fluid sample through the channel of the sample module to and through the measurement and excitation area. After the fluid sample to be examined has passed the measurement and excitation area of the channel, it leaves the sample module through a sample exit.

In embodiments, the channel of the sample module has a support structure or fixation in which it is stably supported and embedded. If the channel is designed, for example, as a tube, capillary or tube, the support structure corresponds to a holder. If the channel is formed, for example, as a structure on a fluidic chip, the fluidic chip itself corresponds to the support structure.

In a preferred embodiment, the at least one sample module separates the at least one magnet and the at least one magnetic field sensor from each other. In a further preferred embodiment, the at least one channel separates the at least one magnet and the at least one magnetic field sensor from one another. In an alternative embodiment, the at least one magnet and the at least one magnetic field sensor are arranged on the same side relative to the sample module, so that the sample module does not separate the two components. However, all components are arranged at a distance from one another in the sense of the invention.

The sample module with the at least one channel is preferably located outside the magnet, very preferably on an end face of the magnet. For example, no coil is wound around the sample module or the channel of the sample module. The size of the magnet can thus advantageously be reduced, as a result of which less space is required. The sample module is therefore also advantageously in the region of the high field gradient and, consequently, a high magnetic field strength change, as a result of which a maximum excitation of the fluid sample to be examined is possible in the measurement and excitation region of the channel.

In a preferred embodiment, the at least one channel of the sample module is made of electrically insulating materials. Because the channel is not electrically conductive, there are advantageously no interactions with the first magnetic field or the modified magnetic field. This means that no additional interference signals are generated.

In one embodiment, the electrically insulating material of the channel is selected from Teflon, polymer, glass or ceramic. The channel is preferably designed as a polymer tube, polymer chip, polymer tube or ceramic capillary.

In one embodiment, the channel consists at least partially of a flexible material. In further embodiments, the channel is designed to be bendable, as a result of which it can advantageously be adapted to the particular structure. A space-saving arrangement of the channel in the sample module is also advantageously possible.

In embodiments, the channel is sterile and can be used, for example, as a disposable module. The fluidic detection system according to the invention can thus advantageously be used for the examination of microorganisms and is therefore of great importance in particular in the field of microbiology and medicine.
In one embodiment, a fluid sample is conveyed through the at least one channel of the sample module. The fluid sample largely comprises fluid media, but also solids. In embodiments, the fluid sample contains at least one transport medium and a large number of objects. This relates in particular to the case when the fluid sample is directed into the detection area of the at least one magnetic field sensor. The objects in the fluid sample are in turn formed by the transport medium and the object medium by an interaction of physical parameters.

The transport medium is designed as a carrier fluid and represents a first, continuous phase with a uniform concentration. The transport medium contains the object medium which forms the objects to be examined or the objects to be counted, which are transported in the transport medium after formation. The transport medium thus serves to transport and flow through the objects of the fluid sample which are guided through the channel. The transport medium has a flow or movement direction in the at least one channel of the sample module.

The transport medium is preferably designed as a liquid or gas. In a further preferred embodiment, the transport medium is designed as an organic liquid, such as an inert oil or an aqueous solution or an electrolyte. In embodiments, the transport medium has at least partially magnetic, metallic and / or ionic properties. Furthermore, the transport medium can be made electrically conductive.

In addition to the transport medium, the fluid medium contains an object medium which, like the transport medium, is passed through the at least one channel of the sample module. The transport medium and the object medium are not miscible with one another.

In a preferred embodiment, the object medium is designed as a liquid. In a further embodiment, the object medium is designed as a gel, gas, steam, solids or combinations of these aggregate states.

In embodiments, the solids of the object medium are particles with at least partially metallic, magnetic and / or ionic properties. In further embodiments, the solids of the object medium are living organisms such as bacteria or active biochemical species. The particles can be dissolved in a liquid such as water or in a gas.

In one embodiment, the objects contained in the fluid medium are formed at a junction of the sample module of the transport medium and the object medium. In a preferred embodiment, the objects are shaped outside the sample module. The fluid medium preferably has a large number of objects. These objects are responsible for modifying the from the at least one magnet generated magnetic field.

The objects represent a second, dispersed phase. In one embodiment, the objects contained in the fluid sample have at least partially magnetic, metallic and / or ionic properties.

These properties of the objects in the fluid sample must be designed in such a way as to produce a measurable change in the modified magnetic field compared to a modified magnetic field of the transport medium.

The transport medium separates the objects from one another, so that the objects are guided through the channel of the sample module at a distance from one another. The objects have no contact with the wall of the channel.

In one embodiment, the objects in the transport medium are guided through the at least one channel of the sample module and have a flow or movement direction. In further embodiments, the direction of flow or movement of the transport medium and the objects of the fluid sample match.

Which type of objects is formed depends on the aggregate states or the nature of the transport medium and object medium.

In one embodiment, the objects are designed as three-dimensional bodies such as drops, bubbles, particles or a combination of these three-dimensional bodies. In a preferred embodiment, the objects are designed as drops or they have a drop shape. This is the case, for example, if an oil is used as the transport medium and a liquid is used as the object medium. The objects are then preferably designed as water-in-oil compositions such as drops, for example as a biochemical liquid in an inert oil. In one embodiment, the objects have a number density.

In a further embodiment, the objects are designed as bubbles, such as gas bubbles. This is the case if a liquid such as water is used as the transport medium and a gas is used as the object medium.

In further embodiments, the objects are designed as particles such as metal particles. This is the case if a liquid such as water is used as the transport medium and a solid is used as the object medium.

It should be noted that the objects (and consequently also the object medium) must differ in their magnetic, metallic and / or ionic properties and in their electrical conductivity from the properties of the transport medium.

In one embodiment, the components of the fluidic detection system according to the invention are each modular. As a result, individual components can be exchanged simply and quickly, for example depending on the individual application or in the case of defects or in the case of repairs, without interacting with the rest of the measuring arrangement or other components of the fluidic detection system. Advantageously, a running measurement does not have to be interrupted by the modular structure. Furthermore, there is advantageously no need for complex readjustment of the fluid detection system according to the invention. For example, if the fluidic detection system according to the invention has at least two sample modules, at least one sample module can also be exchanged in situ during the measurement.

In a preferred embodiment, the components of the fluidic detection system are designed to be detachable and interchangeable.

Depending on the shape of the magnetic field, the fluidic detection system according to the invention can also have several sample modules and / or several magnetic field sensors. “Several” means more than one sample module and / or more than one magnetic field sensor, preferably at least two sample modules and / or at least two magnetic field sensors. In embodiments, the sample modules and / or the magnetic field sensors are preferably arranged or oriented toward the end face of the magnet, on which there is a high field line density and, consequently, a higher change in the magnetic field strength. It should be noted, however, that the sample modules and / or the magnetic field sensors should not be arranged on the same side or end face of the magnet, but rather at different positions, preferably oriented to the first and second end faces of the magnet. In embodiments, the sample modules and / or the magnetic field sensors can be rotated about the magnetic axis of the magnet.

In one embodiment, in the case of several sample modules, the flow directions of the fluid samples are of the same and / or opposite design. In a further embodiment, in the case of a plurality of sample modules, the sample modules are each arranged in parallel and / or not parallel to one another.

Another aspect of the invention relates to a method for operating the fluidic detection system according to the invention for the magnetic-electrical measurement of a fluid sample. The fluid sample is characterized by the measurement.

In the sense of the invention, the fluid sample is conveyed through the channel of the sample module in the flow direction.

In the method according to the invention, a first magnetic field is generated by the at least one magnet. In one embodiment, if the magnet is designed as an electromagnet with at least one coil, the first magnetic field is induced when the coil is energized with direct current or alternating current.

When the fluid sample to be examined with the objects contained therein flows through the detection area of the magnetic field sensor, which includes the measurement and excitation area of the at least one channel of the sample module, which is covered by the first magnetic field, the objects of the fluid sample are in the channel of the Sample module penetrated by this first magnetic field.

According to the invention, when the fluid sample is conveyed through the measuring and excitation area of the at least one channel, the first magnetic field is modified by the fluid sample.

The modified magnetic field arises when the objects of the fluid sample to be examined are excited by the first magnetic field in the measurement and excitation area of the channel and induce a second magnetic field. The first magnetic field is disturbed in that the first and second magnetic fields overlap and generate a modified magnetic field. The modified magnetic field corresponds to an overall magnetic field.
According to the invention, the modified magnetic field is detected by the at least one magnetic field sensor. The modified magnetic field corresponds to a change in the first magnetic field, in particular in terms of strength and shape.
According to the invention, the number, flow rate and / or speed of objects contained in the fluid sample are determined from the change in the modified magnetic field by the fluid sample. In this way, conclusions can be drawn, for example, about the number density of the objects contained in the fluid sample.

According to the invention, the modified magnetic field is used to determine the magnetic, metallic and / or ionic properties of the objects conveyed through the channel and contained in the fluid sample.

On the basis of the detection of the magnetic field sensor, it is determined whether objects are located in the detection area of the magnetic field sensor, are conveyed through it or flow through it, and are therefore in the measurement and excitation area of the channel of the fluidic detection system according to the invention. If this is the case, the change in the signal over time determines the number, flow rate and / or speed at which the objects of the fluid sample are conveyed through the channel of the sample module. The number density of objects, such as the number density of drops, can also be determined in this way. Furthermore, it is possible to determine the magnetic, metallic and / or ionic properties of the objects of the fluid sample.

The strength of the modified magnetic field is directly related to the magnetic, metallic and / or ionic properties of the objects in the fluid sample and thus also to their composition. For example, parameters such as the pH value or the salt concentration of the objects formed have an influence on these properties.

In a further embodiment, as long as no objects of the fluid sample with at least partially magnetic, metallic and / or ionic properties are located in the detection area of the magnetic field sensor or are transported through it or flow through it, which could generate the modified magnetic field, there is no change and thus modification of the first magnetic field, since the magnetic, metallic and / or ionic properties and conditions do not change in the detection range of the magnetic field sensor. Therefore, the measured signal of the magnetic field sensor is not changed and the magnetic field sensor only detects the first magnetic field.

In a preferred embodiment, the magnet is designed as an electromagnet with at least one coil, the at least one magnet designed as an electromagnet with at least one coil and / or the at least one magnetic field sensor being operated with alternating current. In a further embodiment, the at least one coil and / or the at least one magnetic field sensor is operated with direct current. The operation of the magnetic field sensor with alternating current is independent of the design of the magnet.

In a preferred embodiment, the fluidic detection system according to the invention is used to measure and characterize a fluid sample, the fluid sample containing at least one transport medium and a large number of objects, the objects at least partially have magnetic, metallic and / or ionic properties.

In a further preferred embodiment, the method according to the invention is used to measure and characterize a fluid sample, the fluid sample containing at least one transport medium and a large number of objects, the objects at least partially having magnetic, metallic and / or ionic properties.

In a preferred embodiment, the fluidic detection system according to the invention is used to determine the number, flow rate and / or speed of the fluid sample conveyed through the at least one channel and the objects contained therein, and to determine the magnetic, metallic and / or ionic properties of the objects fluid sample transported through the at least one channel.

In a further preferred embodiment, the method according to the invention for determining the number, flow rate and / or speed of the fluid sample conveyed through the at least one channel and the objects contained therein and for determining the magnetic, metallic and / or ionic properties of the objects of the uses the fluid sample conveyed at least one channel.

In a preferred embodiment, the method according to the invention is used in digital microfluidics.

In embodiments, technical impurities such as metal or plastic that are not desired are detected by the fluidic detection system according to the invention. For example, gas can be routed through the channel as a transport medium and any existing technical impurities, which correspond to objects with magnetic, metallic and / or ionic properties such as metal chips or oil drops, can be detected in this way.

In a preferred embodiment, the fluidics detection system according to the invention is used for lab-ob-a-chip applications in micro, milli and nanofluidics and / or digital microfluidics. For example, the kinetics of chemical reactions and metabolic activities of living organisms contained in the objects can be monitored. Resistance to antibiotics with regard to the possibilities for combating hospital germs or germs in the household can thus advantageously be analyzed or new medications can also be tested.

In further embodiments, the fluidic detection system according to the invention is used in the field of biosciences, in particular in bioanalytics and cell cultivation.

The fluidic detection system according to the invention also makes it possible to analyze biological, chemical or medical fluid samples. In the case of a medical analysis, the modular structure advantageously means that only the sample module and not all components of the system need to be replaced.

A contactless measurement of the fluid sample is advantageously possible through the fluidic detection system according to the invention. Due to the modular structure, no complex and time-consuming readjustments of the fluidic detection system are necessary and ongoing measurements do not have to be interrupted.

The new arrangement and dimensioning of the components of the fluidic detection system according to the invention furthermore economically and cost-effectively reduce the time of the sample measurement. By reducing the detection range of the magnetic field sensor, a higher sample throughput or a higher flow rate can advantageously be realized by increasing the number density of the objects in the fluid sample. At the same time, a more compact design of the fluidic detection system according to the invention is also possible, which also results in the possibility of a portable design.

As a result of the new arrangement and dimensioning of the components of the fluidic detection system according to the invention, significantly smaller sample quantities or smaller detection or sample volumes can also be measured. For example, a volume of the fluid sample of just a few nanoliters is sufficient for one measurement - compared to a volume of a few hundred nanoliters as was previously the case in the prior art.

The fluidic detection system also advantageously increases the flow rate by instead of the 30-40 drops / sec known from the prior art [ 7 ] can now be measured by the fluidic detection system according to the invention about 1000 drops / sec.

The fluidic detection system according to the invention ensures reliable and reliable counting and monitoring of the objects in fluid samples, the fluidic detection system according to the invention simultaneously meeting the high requirements of the industry.

Due to the non-optical detection, new, additional information can be provided during the reactions during the measurement, without being affected by tracers or dyes. The fluid samples therefore advantageously do not have to be marked or additionally treated.

Furthermore, no transparent tubes or dyes or optical detectors are advantageously required by non-optical detection. Nevertheless, it is possible to continue to use at least partially transparent materials, as a result of which the user of the fluidic detection system according to the invention remains flexible.

For the implementation of the invention, it is also expedient to combine the above-described configurations, embodiments and features of the claims with one another in a suitable arrangement.

embodiments

The invention will be explained in more detail below using an exemplary embodiment. The exemplary embodiment relates to a fluidic detection system for use in microfluidics and is intended to describe the invention without restricting it.

• 1 einen schematischen Aufbau und Funktionsweise des erfindungsgemäßen Fluidik-Detektionssystems in einer Draufsicht,
• 2 den schematischen Aufbau und Funktionsweise des erfindungsgemäßen Fluidik-Detektionssystems aus 1 mit einer T-Verbindung zur Tropfenerzeugung auf einem Fluidik-Chip,
• 3 einen schematischen Aufbau und Funktionsweise des erfindungsgemäßen Fluidik-Detektionssystems mit einer alternativen Position des Magnetfeldsensors in einer Draufsicht,
• 4 einen schematischen Aufbau und Funktionsweise des erfindungsgemäßen Fluidik-Detektionssystems mit einer anderen alternativen Position des Magnetfeldsensors in einer Vorderansicht,
• 5 einen schematischen Aufbau und Funktionsweise des erfindungsgemäßen Fluidik-Detektionssystems mit zwei Probenmodulen und zwei Magnetfeldsensoren in verschiedenen Positionen in einer Draufsicht,
• 6 einen schematischen Aufbau und Funktionsweise des erfindungsgemäßen Fluidik-Detektionssystems, in welchem Magnetfeldsensor und Spule in alternativen Positionen angeordnet sind, in einer Draufsicht.

The invention is explained in more detail with reference to drawings. Show

• 1 2 shows a schematic structure and mode of operation of the fluidic detection system according to the invention in a top view,
• 2 the schematic structure and operation of the fluidic detection system according to the invention 1 with a T-connection for droplet generation on a fluidic chip,
• 3 1 shows a schematic structure and mode of operation of the fluidic detection system according to the invention with an alternative position of the magnetic field sensor in a plan view,
• 4 1 shows a schematic structure and mode of operation of the fluidic detection system according to the invention with another alternative position of the magnetic field sensor in a front view,
• 5 1 shows a schematic structure and mode of operation of the fluidic detection system according to the invention with two sample modules and two magnetic field sensors in different positions in a plan view,
• 6 a schematic structure and operation of the fluidic detection system according to the invention, in which the magnetic field sensor and coil are arranged in alternative positions, in a plan view.

1 shows a schematic structure and operation of the fluidic detection system according to the invention 1 for use in microfluidics in a top view (sketch not to scale). The fluidic detection system 1 has a sample module 4 which has a channel designed as a polymer tube 5 having. Even in the 3 to 6 is the channel 5 of the sample module 4 designed as a polymer tube. The channel 5 has an internal dimension formed as an inner diameter 6 and an outer dimension formed as an outer diameter 7 on. The inside diameter is 0.5 mm and the outside diameter is 1.58 mm.

A fluid sample is fed into the channel via a sample inlet (not shown in the figure) by means of a syringe (not shown in the figure) 5 of the sample module 4 directed. The fluid sample contains a formed transport medium which has a continuous phase 2 and, by way of example, two objects drawn in as a dispersed phase 3 , Here is the transport medium 2 trained as an inert oil and the objects 3 there are drops formed as water bacteria. This setup with the one sample inlet also applies to the 3 to 6 to. The composition of the fluid sample meets that 2 to 6 to. The longitudinal axis of the channel 8th corresponds to the direction of flow of the fluid sample 9 (shown as arrows) in the channel 5 ,

The fluidic detection system according to the invention 1 further comprises an electromagnet designed as a solenoid 10 and a commercially available magnetic field sensor designed as a Hall sensor 13 which is along the magnetic axis of the coil 11 is arranged so that in the present case the sensor 13 and the coil 10 are positioned opposite and from the sample module 4 spaced apart and separated The coil 10 is along their magnetic axis 11 perpendicular to the longitudinal axis of the channel 8th arranged. The detection range of the magnetic field sensor 13 and the first face of the coil 16 point directly to the sample module 4 ,

The dimensioning of the coil perpendicular to its magnetic axis 15 corresponds exactly to the detection range of the magnetic field sensor 13 , Furthermore, the dimensioning of the coil is perpendicular to its magnetic axis 15 smaller than the outside dimension of the channel 5 ,

The fluid sample to be examined is passed through the channel 5 of the sample module 4 in the flow direction 9 to and through the measurement and excitation area of the channel 5 of the fluidic detection system 1 directed.

The sink 10 is operated with alternating current and induces a first exciting magnetic field designed as an alternating field 12 , The objects contained in the fluid sample 3 have magnetic properties and are measured and excited by the first magnetic field 12 excited and induce a second magnetic field 14 , The first magnetic field overlaps 12 and the second magnetic field 14 and this overlay is used as a modified magnetic field by the magnetic field sensor 13 measured. The magnetic field sensor detects 13 the strength of the modified magnetic field. To illustrate, instead of the modified magnetic field in the sense of a better representation in the 1 to 6 the first magnetic field 12 and the second magnetic field 14 of magnetically trained objects 3 shown. This representation would also refer to the metallic or ionic properties of the objects 3 hold true. The magnetically formed properties of the objects can be derived from the amount of the signal 3 be determined. From the change in the signal over time, the number and flow rate of the objects 3 be determined.

After the fluid sample passes the measurement and excitation area of the channel 5 has passed, it leaves the sample module 4 through a sample outlet (not shown in the figure).

2 shows the schematic structure and operation of the fluidic detection system according to the invention 1 for use in microfluidics 1 with a T-connection shown in the figure above for generating objects designed as drops 3 in a sample module designed as a fluidic chip with microfluidic structures 4 (Sketch not to scale). The sample module designed as a fluidic chip has 4 a channel designed as a structure 5 with an internal dimensioning 6 on.

The sample module 4 has two sample inlets (not shown in the figure) through which the fluid sample in each case into the channels 5 of the sample module 4 is directed. A connection designed as a T-connection (above in 2 shown) of the sample module 4 through which the formation of the objects formed as drops 3 he follows. The objects become from the T-connection 3 in the direction of flow of the fluid sample 9 through the channel 5 of the sample module 4 to and through the measurement and excitation area of the channel 5 of the fluidic detection system 1 directed. The rest of the structure and the measuring principle are the same 1 identical. After the fluid sample passes the measurement and excitation area of the channel 5 has passed, it leaves the sample module 4 through a sample outlet (not shown in the figure).

3 shows a schematic structure and operation of the fluidic detection system according to the invention 1 for use in microfluidics with an alternative position of the magnetic field sensor 13 in a top view (sketch not to scale).

The magnetic field sensor is located here 13 not on the magnetic axis of the coil 11 , but is shifted to this and on the side of the coil 10 positioned, which also changes the channel 5 of the sample module 4 not between the coil 10 and the magnetic field sensor 13 located. The magnetic field sensor 13 is shifted here, but the detection range of the magnetic field sensor 13 to the sample module 4 has. Here is the magnetic field sensor 13 adjusted in all three spatial directions and angles of rotation.

4 shows a schematic structure and operation of the fluidic detection system according to the invention 1 for use in microfluidics in the front view with another alternative position of the magnetic field sensor 13 (Sketch not to scale).

The magnetic field sensor is also located here 13 not on the magnetic axis of the coil 11 , but is shifted to this, but opposite from the coil 10 ,

5 shows a schematic structure and operation of the fluidic detection system according to the invention 1 for use in microfluidics with two sample modules arranged parallel to each other 4 and two magnetic field sensors 13 in different positions in a top view (sketch not to scale). The flow directions of the fluid sample 9 are trained in opposite directions.

There is one sample module each 4 which each have a channel 5 has, with a magnetic field sensor 13 on one side of the coil 10 , The first end face of the coil 16 on the left in 5 to the first, left in 5 arranged sample module 4 and the second face of the coil 17 on the right in 5 points to the second, right in 5 arranged sample module 4 , The magnetic field sensors 13 are each in different positions with respect to spatial directions and angles of rotation and opposite each other from the coil 10 arranged.

In this embodiment, the coil is advantageous 10 induced first magnetic field 12 more effective used because the two sample modules 4 with the respective measurement and excitation ranges of their channels 5 each on the first face 16 or the second end face 17 the coil 10 arranged and are thus in the area of a high field line density.

6 shows a schematic structure and operation of the fluidic detection system according to the invention 1 in which magnetic field sensor 13 and coil 10 are arranged in alternative positions in a top view (sketch not to scale).

In this construction is the coil 10 along their magnetic axis 11 not perpendicular to the longitudinal axis of the channel 5 of the sample module 4 arranged, so that the measurement signal can not be fully used. The magnetic field sensor 13 again is along the magnetic axis of the coil 11 arranged and is from the coil 10 , as in the 1 . 2 . 4 and 5 shown by the sample module 4 Cut.

Non-patent literature cited:

• [1] Whitesides, GM, The origins and the future of microfluidics, Nature, Vol. 442, 2006, 368-373
• [2] Baraban, L., Bartholle, F., Salverda, MLM, Bremond, N., Panizza, P., Baudry, J., de Visser, JAGM, Bibette, J., Microfluidic droplet analyzer for microbiology, Lab Chip, Vol. 11, 2011, 4057-4062
• [3] Mark, D., Haeberle, S., Roth, G., von Stetten, F., Zengerle, R., Microfluidic lab-on-a-chip platforms: requirements, characteristics and applications, Chemical Society Reviews, Vol. 39, 2010, 1153-1182
• [4] Vasan, ASS, Mahadeo, DM, Doraiswami, R., Huang, Y., Pecht, M., Point-of-care biosensor system, Frontiers in bioscience, S5, 2013, 39-71
• [5] Murran, MA, Najjaran, H., Capacitance-based droplet position estimator for digital microfluidic devices, Lab Chip, Vol. 12, 2012, 2053-2059
• [6] Trivedi, V., Doshi, A., Kurup, GK, Ereifej, E., Vandevord, PJ, Basu, AS, A modular approach for the generation, storage, mixing, and detection of dropletlibraries for high throughput screening, Lab Chip, Vol. 10, 2010, 2433-2442
• [7] Karnaushenko, D., Baraban, L., Ye, D., Uguz, I., Mendes, RG, Rümmeli, MH, de Visser, JAGM, Schmidt, OG, Cuniberti, G., Makarov, D., Monitoring microbial metabdites using an inductively coupled resonance circuit, Scientific Reports, Vol. 5, 2015, 12878
• [8th] Santos, RM, & Kawaji, M. (2010). Numerical modeling and experimental investigation of gasliquid slug formation in a microchannel T-junction. International Journal of Multiphase Flow, 36 (4), 314-323

LIST OF REFERENCE NUMBERS

1

Fluidic detection system

2

transport medium

3

object

4

sample module

5

channel

6

Internal dimensioning of the channel

7

External dimensioning of the channel

8th

Longitudinal axis of the channel

9

Flow direction of the fluid sample

10

Kitchen sink

11

Magnetic axis of the coil

12

First magnetic field

13

magnetic field sensor

14

Second magnetic field

15

Dimensioning of the coil perpendicular to its magnetic axis

16

First face of the coil

17

Second face of the coil

QUOTES INCLUDE IN THE DESCRIPTION

This list of documents listed by the applicant has been generated automatically and is only included for the better information of the reader. The list is not part of the German patent or utility model application. The DPMA assumes no liability for any errors or omissions.

Non-patent literature cited

• Whitesides, G.M., The origins and the future of microfluidics, Nature, Vol. 442, 2006, 368-373 [0136]
• Baraban, L., Bartholle, F., Salverda, MLM, Bremond, N., Panizza, P., Baudry, J., de Visser, JAGM, Bibette, J., Microfluidic droplet analyzer for microbiology, Lab Chip, Vol. 11, 2011, 4057-4062 [0136]
• Mark, D., Haeberle, S., Roth, G., von Stetten, F., Zengerle, R., Microfluidic lab-on-a-chip platforms: requirements, characteristics and applications, Chemical Society Reviews, Vol. 39, 2010, 1153-1182 [0136]
• Vasan, A.S.S., Mahadeo, D.M., Doraiswami, R., Huang, Y., Pecht, M., Point-of-care biosensor system, Frontiers in bioscience, S5, 2013, 39-71 [0136]
• Murran, M.A., Najjaran, H., Capacitance-based droplet position estimator for digital microfluidic devices, Lab Chip, Vol. 12, 2012, 2053-2059 [0136]
• Trivedi, V., Doshi, A., Kurup, GK, Ereifej, E., Vandevord, PJ, Basu, AS, A modular approach for the generation, storage, mixing, and detection of dropletlibraries for high throughput screening, Lab Chip, Vol. 10, 2010, 2433-2442 [0136]
• Karnaushenko, D., Baraban, L., Ye, D., Uguz, I., Mendes, RG, Rümmeli, MH, de Visser, JAGM, Schmidt, OG, Cuniberti, G., Makarov, D., Monitoring microbial metabdites using an inductively coupled resonance circuit, Scientific Reports, Vol. 5, 2015, 12878 [0136]
• Santos, R.M., & Kawaji, M. (2010). Numerical modeling and experimental investigation of gasliquid slug formation in a microchannel T-junction. International Journal of Multiphase Flow, 36 (4), 314-323 [0136]

Claims (15)

Fluidic detection system for the magnetic-electrical measurement of a fluid sample in micro, milli and nanofluidics, comprising the following components: at least one sample module which has at least one channel which is designed to convey a fluid sample along a flow direction, the longitudinal axis of the channel corresponding to the flow direction, - At least one magnet with a magnetic axis, the magnet being designed to generate a magnetic field in a measurement and excitation area of the channel, the dimensioning of the magnet perpendicular to its magnetic axis being at least one dimension smaller than the external dimensioning of the channel perpendicular to it Longitudinal axis is at least one magnetic field sensor, which is designed to detect a magnetic field and whose detection area includes the measurement and excitation area of the channel of the sample module, the sample module, the magnetic field sensor and the magnet being arranged at a distance from one another, wherein the detection range of the magnetic field sensor corresponds to the dimensioning of the magnet perpendicular to its magnetic axis in at least one dimension. Fluidic detection system according to Claim 1 , characterized in that the at least one magnet is arranged with its magnetic axis perpendicular to the longitudinal axis of the channel of the sample module, the magnetic axis of the magnet intersecting the longitudinal axis of the channel. Fluidic detection system according to Claim 1 or 2 , characterized in that the magnetic axis of the at least one magnet intersects the at least one magnetic field sensor. Fluidic detection system according to one of the Claims 1 to 3 , characterized in that the sample module separates the at least one magnet and the at least one magnetic field sensor. Fluidic detection system according to one of the Claims 1 to 4 , characterized in that the at least one channel of the at least one sample module is designed as a tube, capillary, tube or as at least one structure on a fluidic chip. Fluidic detection system according to one of the Claims 1 to 5 , characterized in that the at least one channel of the sample module is made of electrically insulating materials. Fluidic detection system according to one of the Claims 1 to 6 , characterized in that the components of the fluidic detection system are detachable and interchangeable. Fluidic detection system according to one of the Claims 1 to 7 , characterized in that the at least one magnet is designed as an electromagnet with at least one coil or as a permanent magnet. Fluidic detection system according to one of the Claims 1 to 8th , characterized in that the at least one magnetic field sensor is designed to detect the strength or the gradient of the magnetic field. Fluidic detection system according to one of the Claims 1 to 9 , characterized in that the at least one magnet and / or the at least one magnetic field sensor are designed to be adjustable and displaceable. Method for operating a fluidic detection system for the magnetic-electrical measurement of a fluid sample according to one of the Claims 1 to 10 , wherein a first magnetic field is generated by the at least one magnet, and the fluid sample is conveyed through the at least one channel of the sample module in the direction of flow, wherein when the fluid sample is conveyed through the measurement and excitation area of the at least one channel, the first magnetic field through the fluid Sample modified, wherein the modified magnetic field is detected by the at least one magnetic field sensor, and from the change in the modified magnetic field by the fluid sample, the number, flow rate and / or speed and the magnetic, metallic and / or ionic properties of the fluid Objects contained in the sample can be determined. Procedure according to Claim 11 , characterized in that the at least one magnet is designed as an electromagnet with at least one coil and the at least one magnet designed as an electromagnet with at least one coil and / or the at least one magnetic field sensor is operated with alternating current. Use of a fluidic detection system as in one of the Claims 1 to 10 defined, or a method as in one of the Claims 11 or 12 defined, for measuring and characterizing a fluid sample, the fluid sample at least one transport medium and a plurality of Contains objects, the objects at least partially having magnetic, metallic and / or ionic properties. Using a fluidics detection system such as one of the Claims 1 to 10 defined, or a method as in one of the Claims 11 or 12 defined, for determining the number, flow rate and / or speed of the fluid sample conveyed through the at least one channel and the objects contained therein, and for determining the magnetic, metallic and / or ionic properties of the objects of the fluid sample conveyed through the at least one channel , Using a fluidics detection system such as one of the Claims 1 to 10 defined, or a method as in one of the Claims 11 or 12 defined for Lab-ob-a-Chip applications in micro, milli and nanofluidics and / or digital microfluidics.

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