DE102008035285A1 - Extraction of active substances from group of anthraquinone and phenols and/or polyphenols from a plant, where new plant variety Igniscum (CPVO 2007/0149) or Candy (CPVO 2007/1958) registered to variety protection is requisitioned as plant - Google Patents

Abstract

The invention relates to a method for obtaining active substances from a plant, according to the preambles of claims 1, 6 and 7. In order to find a suitable approach in connection with a suitable plant as an active ingredient supplier, in which and with which a large-scale extraction of said plant active ingredients is possible, the invention proposes that the new plant variety registered for plant variety IGNISCUM (CPVO 2007/0149) or CANDY (CPVO 2007/1958) is used as a plant.

Description

The The invention relates to a process for the production of active ingredients a plant, as well as raw materials and uses, according to the generic terms of the claims 1, 8, 9 and 10.

A Plant of the genus Polygonacea, for example, the Polygonum Sachalinense. From it are known ingredients of various kinds, For example, substances from the group of anthraquinones, such as Emodin and Physcion. About that In addition, their range of active ingredients in terms of quantity valuable substances are of little interest. But even in case the appearance of valuable ingredients has to be considered that the known polygonaceans are extremely invasive plants that can not be grown in plantation cultivation. Their invasiveness is over theirs Seeds and over given extremely fast proliferating Wurzelausläufer. For this reason is a controlled cultivation of Polygonacea or Fallopia Sakhalinense not possible for drug extraction.

insofar represent these polygonacea species in those areas where she originally were not native to so-called neophytes dar. The cultivation of such known neophytes remain extreme because of their invasiveness despite valuable ingredients problematic, if not prohibited.

Further Methods are known in which shredded plant material and by means of alcohols such as ethanol or methanol and others the plant active ingredients detached from the same become.

There because of their invasiveness, the aforementioned plants are not planted in large quantities can be grown or may, is sufficient plant material of this kind for a large-scale exhaustion said plant active ingredients from said plant not to Available.

It it is also known that the substance compounds of the anthraquinones but also phenols or polyphenols are plant-derived substances, the one known generate high crop protection effect. Plants rich in such Substances or compounds are usually show a high self-protection against fungal infestation and / or other plant diseases. To some extent, too to detect a correspondingly high resistance to pest infestation, the but on the preventive Effect of these plant-based substances.

Of the The invention is therefore based on the object, a suitable approach in conjunction with a suitable plant as an active ingredient supplier to find, in which and with which a large-scale extraction of said Plant active ingredients possible is.

The Asked object is in a method of the generic type according to the invention the characterizing features of claim 1 solved.

Further advantageous embodiments are in the dependent claims 2 to 7 indicated.

in the With regard to an extract of active substance or raw material as raw material is the task according to the invention by the characterizing Features of the claims 8 solved.

Further Various uses are given in the remaining claims.

The new plant varieties Igniscum 2007/0149 CPVO and Candy CPVO 2007/1958 are available over the 2E Renewable Energies GmbH, Trimburgstraße 2, 81249 Munich. There they are led under the brand name Igniscum or Igniscum-basic, and Igniscum-Candy.

The The genus of plants from which the new plant varieties originate is the Genus of Polygonacea. In contrast, the plants are in their Properties through many years Selection breeding partly changed considerably.

Further Information on the plant is available on the publication number mentioned above the web page of the CPVO (Community Plant Variety Office) in Angers, France.

So Essentially two types are described in the prior art, namely the Reynoutria Sachalinense and the Reynoutria Japonica, also Polygonum Cuspitatum called.

The Species or sorts Sakhalinense and Japonica are as described above, extremely invasisv, and above all her very aggressively wild-driving roots, but also about her Blossoms- and fruit stalls.

The mentioned new plant varieties Igniscum CPVO 2007/0149 and Candy CPVO 2007/1958, on the other hand, are based on a spontaneously mutated from these strains in nature new plant, which was immediately isolated upon observation of this mutation. This mutation initially brought all biological systems. However, in order to reduce the invasiveness of the plant, another breeding selection was added, leading to sterility (empty fruiting) and overgrown root shoots. The measures of rigorous Selection then gradually led to the expression of these special properties. In the process, natural mutation processes were used, which supported the selection and were controlled by the same. The properties of seed formation and root shoots were virtually completely reduced at the conclusion of this laborious selection work. This has the consequence that the plant is exclusively only vegetatively reproducible. The property of the invasiveness of the parent varieties has thus regressed sustainably, and the new plant is now suitable for plantation cultivation.

Further Thus, all propagated new plants are genetically identical to the mother plant from which the new seedlings are extracted. This in turn ensures a high resistance, Varietal purity and homogeneity the seedlings of this new plant variety thus produced. Through her sterility It also prevents crossbreeding with other varieties comes in nature.

After this these properties were sufficiently stabilized, did the Plant variety protection applications on an international basis.

Dry Analyzes of these new plant varieties showed higher concentrations of the mentioned during breeding Substance groups of the anthraquinones and polyphenols as the original sorts of Sakhalinense and Japonica. The optimization of these substance concentrations stood while breeding, d. h Selection phase in the foreground. Thus, the non-invasiveness became synchronous increased for drug optimization.

This non-invasiveness and the drug optimization is optimal with the new plant varieties mentioned succeeded and was also not obvious. For breeding basically shift all plant characteristics, so that the synchronous expression of several plant characteristics of mastering a statistical chaotic system equals.

It was thus surprising that both properties could be optimized at the same time.

one following another chemical analytical observation forms the new Plant variety from a plurality of glucose compounds. This attribute was therefore also considered selectively. At another legal expression of this Glykosearten was therefore the increased glycosylation of the substance groups the phenols and polyphenols sought. This has succeeded and this too Property was developed in further selection, so that with the new plant varieties also a much higher proportion on glucose compounds as usual is obtained.

intention In this case, the extraction of said substance groups of the phenols, To facilitate polyphenols and anthraquinones from the plant.

For this was in the further selection of the plant made sure that the Glycosation of the above substance groups as far as possible already within the plant itself.

In order to These valuable substances can be extracted in high quantities from the plant material now by alcoholic extracts but also extract by alcohol-water mixtures. The accumulated glycoses, or the glycosides now obtained by their OH groups polar and therefore both alcohol soluble as partially water-soluble.

The so bred Plant varieties are therefore for a large-scale Extraction of valuable ingredients very well suited, even because their ingredients are easily extractable.

Especially interesting here is the cumulative effect of achieved high Mass yield of said plant, in connection with the expression relative high concentrations of said chemical ingredients.

• – hoher Massenertrag der neuen Pflanzensorten bei eliminierter Invasivität,
• – kombiniert mit den genannten chemischen Inhaltsstoffen,
• – kombiniert mit der Ausprägung der Inhaltsstoffe in glykosierter Form,
• – kombiniert mit einer Auszugsmethode mit Alkohol und/oder Alkohol-Wasser-Gemischen

eine einzigartige Innovation auf diesem technischen Gebiet dar.Thus, the combinations of the features of the invention

• High mass yield of the new plant species with eliminated invasiveness,
• - combined with the mentioned chemical ingredients,
• - combined with the expression of the ingredients in glycated form,
• - combined with an extraction method with alcohol and / or alcohol-water mixtures

a unique innovation in this technical field.

The said substances or groups of substances play a significant role as Basic material for pharmaceutical, cosmetic and other products.

The Plant forms significantly high concentrations of these under stress Substances, namely the anthraquinones and phenols and polyphenols right down to the foliage out.

The strong glycation is also achieved that the occurrence of stress factors, such as strong sunlight, drought, excessive daylight hours, exposure to insect attack and even bite the plant retrieve the active ingredients from the depot, ie from the root and stalk area extremely fast and can activate. The glycation supports the osmosis for the diffusion of these substances to the required plant location. In the selection of breeding methods were used, in which exactly these stress conditions were created artificially and so the properties of this property best performing plants could be selected and developed accordingly.

at These stressful situations were already visual changes to observe, some of which were reversible again after the stress situation was eliminated again.

So The plant seedlings of the mentioned new varieties contribute New shoot and in strong sunlight exposure during the Re-emergence a significant red coloration, which is reversible proved when the sunlight exposure decreased again. For this Sunlight reaction effect or sunscreen effect are known to be optical responsible for active substances. For this purpose, the substance groups of To name phenols and polyphenols, which are also used in dermatological Sunscreens are used.

A further significant observation is the self-resistance to fungal infestation and also against insect infestation. Here are from the substance groups of Antrachinones to call Emodin and Physcion, here also as Emodin glycose and Physcion glycose are present. These substances play an essential role in plant protection products and plant additives.

core of the invention The procedure is thus that as plant the new ones for plant variety protection registered plant variety IGNISCUM (CPVO 2007/0149) and Candy (CPVO 2007/1598), which developed over selection breeding with eliminated invasiveness provides a high mass yield, in which by Auslesezüchtung the Drug groups of anthraquinones and phenols and / or polyphenols in predominantly glycated Form, and with the aid of an alcohol (ethanol, methanol, Hexanol) and / or an alcohol-water mixture.

The Combination of said features first leads to said significant Advantage.

In Another advantageous embodiment is indicated that from the Drug group of the anthraquinones essentially emodin and / or Physcion is represented, and that they contain in glycated form are. By the selection-based glycation, as mentioned above has been developed, the substances as a whole are to a much greater extent alcoholic and water-soluble. This property became breeding technology also advantageously further developed.

By Glycation, the active ingredients are not only easily triggered, but also considerably more complete as that would be the case in non-glycated form.

The preferably full release The said active substance in turn has a considerable causal effect. These anthraquinones and phenols / polyphenols have come into use this plant material for biogas production is more of a gas-deterrent effect.

As you know Antrachinones like Emodin and Physcion, as well as polyphenols are a bacteriocidal Effect. So if these substances are more significant in plant material Way, then leads while this increased Self-protection of the plant against disease infestation, but also that due This bactericidal substances of the fermentation process, at least initially inhibited would. If However, these substances previously triggered d. H. from the plant substrate removed, so be open-minded, the biogas process can advantageously fed to a substrate which does not contain these gas inhibitors anymore.

The said plants Igniscum and Candy ferment with excellent Values at a gas production of 550 to 850 liters / kg dry matter. However, it has been shown that digestion and removal of the said Substances further increases these gas values.

Consequently creates a very significant and unobvious double benefit. On the one hand, the disrupted active ingredients are valuable raw materials, which can be further processed, for example, as plant protection products and plant adjuvants, on the other hand They then from the as fermentation substrate used plant residue material, resulting in a significantly increased gas generation rate leads.

This ultimately leads to that the said new plant variety developed by the Characteristics and by the chronology of the procedure double can be used. This opens up completely new perspectives in the systematic exploitation of such new biomass plants.

there There is a significant advantage in that the said plant is not a food crop and via said source, 2E Eneuerbare Energie GmbH & Co KG Trimburgstrasse 2, 81249 Munich, or Conpower GmbH & Co KG, Brunhamstrasse 21, 81249 Munich sufficiently to disposal stands.

This is taken into account in the further embodiment by the said Active ingredients by extracting first removed and chemically common Methods are processed while the remaining plant material as input substrate for fermentation or fed directly to a biogas plant.

In Another advantageous embodiment is specified that at least a specified from said drug groups active ingredients as a raw material for Plant protection products or plant additives is used.

In Another advantageous embodiment is specified that the base material preferably Emodin and / or Physcion is, which from the new Plant variety IGNISCUM (CPVO 2007/0149) or from the new plant variety CANDY (CPVO 2007/1958).

Further is a pesticide which can be prepared with corresponding advantage or a plant adjuvant, which or the raw material according to claim 6 contains.

there has by experiments an interesting observation conspicuous and shown repeatable.

The from the plant extract according to the invention prepared crop protection agent has known, on anthraquinones and / or phenols based plant protection products higher Preventive effect. For the Case that other plants are treated with it is also a remarkably fast Healing effect on the plant in relation to leaf diseases etc achievable.

One Pesticides produced on this basis of said base thus substitutes in many cases the use of questionable pesticides.

The entire concept according to the invention, d. H. Process, basic material, plant protection product / plant adjuvant, as well as the withdrawal of these substances before entry of the substrate in a biogas plant thus provides a consistent, significantly beneficial ecological Overall concept. These may read, "From the extraction of an ecological Plant basic material up to the optimized Biogassubstratgewinnung. "

The Combination of plant properties and procedures, as well the chronology of the measures For this reason, they deliver a surprising Overall result. Important is always the consideration the removal of the gas inhibitors before the biogas process. Whereby the "gas-dryers" turn highly valuable substances are those of a further utilization than natural chemical Supplied basic material become.

All in all this creates the possibility of one consortial benefits to one and the same planted crop.

This represents in particular for biomass energy production has significant commercial benefits represents.

This is particularly against the background to see that said yield a high mass yield to new plant varieties (300 to 600 Tons of fresh mass per hectare / year). Particularly noteworthy is that the plants are NOT food plants.

This helps as well the conflicting interests of competing food and to eliminate energy crops.

Next the production of a Auszugsgrundstoffes from the said new Plant varieties it is also possible to produce a plant adjuvant by that the same from regrind of the rhizomes and / or the stem and / or the leaves the plant variety IGNISCUM (CPVO 2007/0149) or CANDY (CPVO 2007/1958) exists, with a fineness of the ground material with a particle size of smaller than 1 millimeter.

This Grist can then either separately or with a fertilizer applied. The ground material can also be mixed with water be sprayed on plants. Overall, it thus provides a plant additive with good effect and with considerable ecological compatibility.

A Design of a complex process with a combination of extraction methods and subsequent reuse is shown in the drawing.

The Drawing shows a schematic representation of the procedure, in which technically also the subsequent energy production the substrate residue is indicated.

In a first step is the plant material in the manner indicated the two optionally used new varieties Igniscum and Candy harvested.

Thereafter, the crop, preferably the whole plant, is comminuted and placed in a chemical separation stage. This includes physical comminution of the plant and adding Alcohols, as described above, or an alcohol-water mixture, or first alcohol, and then pure water, if necessary as a distillate, so that the glycated substances present in the plant material are discharged and then chemically / physically separated.

The discharged active substances are used as basic substances for the specified Separate separation. The remaining plant substrate becomes preferably dried and a fermentation stage of a biogas plant supplied and involved in the gas generation process.

By The previously taken active ingredients are the otherwise annoying gas inhibitors removed, and the gas generation process runs faster and at higher gas generation rate from.

Of the remaining remainder after biogas production can be restored to the acreage as a fertilizer to be led back. This results in a closed ecological process, which ultimately also is commercially advantageous.

Claims (10)

Process for the production of active substances from the group of anthraquinones and phenols and / or polyphenols from a plant, characterized in that the new plant variety IGNISCUM (CPVO 2007/0149) or CANDY (CPVO 2007/1958) filed for plant variety protection is used as the plant. Method according to claim 1, characterized in that that the plant variety IGNISCUM (CPVO 2007/0149) or CANDY (CPVO 2007/1958) on selection breeding with eliminated invasiveness provides a high mass yield, in which by Auslesezüchtung the Drug groups of anthraquinones and phenols and / or polyphenols in predominantly glycated form are developed, and with the help of an alcohol (Ethanol, methanol, hexanol) and / or an alcohol-water mixture be pulled out. Method according to claim 1, characterized in that that from the drug group of the anthraquinones essentially emodin and / or Physcion is represented, and that these are glycated Form are pulled out. Method according to claim 1 or 2, characterized that said plants at least one or more days before Harvesting can be put under biological stress. Method according to claim 4, characterized in that that the biological stress is due to increased exposure to UV light and / or the reduction of the water supply is generated, and then the harvesting when a significant reddish color change occurs is made. Method according to one of the preceding claims, characterized characterized in that the said active substances initially removed by extraction and chemically common Methods are processed while the remaining plant material is supplied as an input substrate of a biogas plant. Method according to one of the preceding claims, characterized characterized in that at least one active ingredient from said active ingredient groups as a raw material for Plant protection products or plant additives is used. Basic substance, in particular the drug group of anthraquinones or phenols or polyphenols derived from a plant thereby characterized in that the base material is preferably emodin and / or Physcion is, which from the new plant variety IGNISCUM (CPVO 2007/0149) or from the new plant variety CANDY (CPVO 2007/1958) is. Plant protection product or plant adjuvant, thereby characterized in that the pesticide or the plant adjuvant the base material according to claim 8, ie emodin and / or Physcion, which from the new plant variety IGNISCUM (CPVO 2007/0149) or from the new plant variety CANDY (CPVO 2007/1958). Plant adjuvant, characterized in that the same from regrind of rhizomes and / or stalk and / or the leaves the plant variety IGNISCUM (CPVO 2007/0149) or CANDY (CPVO 2007/1958) exists, with a fineness of the ground material with a particle size of smaller than 1 millimeter.