DE102005020457A1 - Use of non-pathogenic sporulating bacteria for inhibiting the growth of infectious germs on the udder and/or teat of a lactating mammal - Google Patents

Abstract

Use of non-pathogenic sporulating bacteria for the treatment of the udder and/or teat of a lactating mammal, is new. An independent claim is included for a preparation for the prevention and treatment of infections of the udder and/or teat of a lactating mammal, comprising at least one non-pathogenic sporulating bacterium. ACTIVITY : Antibacterial. MECHANISM OF ACTION : None given.

Description

The The present invention relates to a composition for inhibition the growth of pathogenic bacteria on the teats of a lactating mammal, a method of inhibiting the growth of pathogenic bacteria lactating mammals and the use of non-pathogenic, spore-forming bacteria and / or their spores.

Bovine, Sheep and goats are becoming prevalent today milked by using automatic milking techniques. By many years breeding success Animals trimmed for high milk production are in the area of the mammary glands and Udder very susceptible to infection. The susceptibility to infection is due to heavy use of the teats during milking and through constantly environmental factors affecting the teats such as wind, rain and solar radiation elevated.

By this permanent Exposure to the teats often causes mastitis in the animals, as "subclinical" or "clinical" mastitis in the Literature will be described. Depending on the strength of the infection of the diseased Udder areas there is a slight to drastic increase somatic Cells in the milk. The increase somatic cells in the milk over the measure of healthier Udder (200,000 cells per ml) is undesirable because it affects the quality of the milk for the limits further processing steps or the milk in extreme cases inedible can be. A drastic increase somatic Cells in the milk are often associated with fever (clinical mastitis) until the loss of the animal by emergency slaughter.

A Cause for such infections is the immigration of udder pathogens such as Pseudomonas aeruginosa, Escherichia coli, Streptococcus ube ris, Streptococcus dysagalactiae, Streptococcus agalactiae, Staphylococcus aureus or Enterococcus hirae.

The biggest danger the udder infection is immediately after the milking process. In This time, the bar channel is open, causing it to germinate possible from the environment of the udder is to immigrate into the teat canal and cause infections.

for Avoiding or reducing the risk of infection, the teats the milked animals after each milking process with udder-dip preparations treated. Treatment is either by dipping or by spraying the teats with the disinfectant solution. By this measure are the germs present on the teat skin within 10 minutes killed, whereby a reduction of the risk of infection on the opened bar channel.

For example EP-A-90 205 describes a preparation for udder care and teat disinfection Dairy cattle being as watery Increased viscosity emulsion applicable is and among other things, 800 to 1200 ppm of iodine in the form of a disinfectant contains active iodine complex. As disinfecting iodine complexes, adducts of iodine with polyglycol ethers or used on polyvinylpyrrolidone.

Out WO-A-94/23581 teat disinfectants are known, many the above-mentioned disadvantages do not have: they contain a film-forming component consisting of polyvinyl alcohol, a polymeric Thickener and an antimicrobial nonionic iodine complex. When nonionic iodine complex, for example, adducts of iodine Polyethylene glycol, polypropylene glycol or copolymers thereof.

Of the Disadvantage of this from the prior art for the treatment of teats described preparations is, however, that the active substances, in particular then, when applied before milking, get into the milk can.

EP-A-1 312 371 beats to use bacteria of the type Brachybacterium as antibacterial agent. However, these bacteria are, depending on weather conditions, only limited on the surfaces the teats survive. Especially under particularly dry, particularly hot or particularly cold environmental conditions The bacteria of the type Brachybacterium die quickly, so that reapplying an udder-dip preparation containing these bacteria is required.

Of the Present invention was based on the object resulting from the Overcome prior art disadvantages.

Especially The present invention has the object, a for the treatment of inflammations in lactating mammals to provide suitable composition by a particularly high stability is tagged so that they only possible once a week must be applied.

The present invention also an object of the invention to provide a method for inhibiting the growth of pathogenic bacteria on lactating mammals, which compared with her conventional process is less expensive and at the same time the milk quality is not adversely affected.

a Contribution to the solution These objects provide a composition for inhibiting growth pathogenic bacteria on the teats of a lactating mammal, comprising a skin care agent, non-pathogenic, spore-forming Bacteria and / or their spores and optionally a carrier.

Under "pathogenicity" in the sense of the present Invention becomes the constant property of a special bacteria understood in a lactating mammal, preferably in cattle, To be able to cause disease symptoms. Under a "non-pathogenic" bacterium in the sense the present invention is therefore understood to mean a bacterium which in a lactating mammal, preferably in a cattle, does not cause any signs of illness.

Under "spore-forming" bacteria in the sense of present invention are preferably understood as bacteria, which are capable of transforming from their vegetative form into a Heat, cold and to transition to chemicals-resistant, non-vegetative, chronic stages, from this non-vegetative end stage again vegetative Bacteria can germinate.

It is preferred according to the invention that the composition is at least 1 × 10 ⁶ , preferably at least 1 × 10 ⁷ , particularly preferably at least 1 × 10 ⁸ , moreover preferably at least 1 × 10 ⁹ and most preferably at least 1 × 10 ¹⁰ non-pathogenic, spore-forming bacteria and / or spores of these bacteria per gram of the composition. The expression "and / or" means that the sum of (vegetative) bacterial cells and (non-vegetative) pores should reach the specified minimum values According to a particular embodiment of the composition according to the invention, this is based on at least 25%, preferably at least 50%, particularly preferably at least 75%, based on the total amount of (vegetative) bacteria and (non-vegetative), but most preferably exclusively on spores.

Furthermore, it is preferred according to the invention that the non-pathogenic, spore-forming bacteria and / or spores are bacteria or spores selected from the group consisting of Bacillus and Clostridium, the following bacterial strains being particularly preferred: Bacillus acidocaldarius, Bacillus acidoterrestris, Bacillus acidovorans, Bacillus aeolius, Bacillus agar-exedens, Bacillus agaradhaerens, Bacillus agarexedens, Bacillus agrestis, Bacillus agri, Bacillus alcalophilus, Bacillus alcalophilus subsp. Halibutans, Bacillus alginolyticus, Bacillus alpinus, Bacillus alvei, Bacillus aminovorans, Bacillus amyloliquefaciens, Bacillus amylolyticus, Bacillus aneurinilyticus, Bacillus apiarius, Bacillus aquimaris, Bacillus arenosi, Bacillus arseniciselenatis, Bacillus arvi, Bacillus atrophaeus, Bacillus azotofixans, Bacillus azotoformans, Bacillus badius, Bacillus barbaricus, Bacillus bataviensis, Bacillus bellus, Bacillus benzoevorans, Bacillus betainovorans, Bacillus borstelensis, Bacillus brevis, Bacillus butyricus, Bacillus caldolyticus, Bacillus caldotenax, Bacillus caldovelox, Bacillus caldoxylolyticus, Bacillus calidolactis, Bacillus capri, Bacillus carotarum, Bacillus centrosporus, Bacillus cereus , Bacillus cereus var. Mycoides. Bacillus cereus var. Thuringiensis, Bacillus chitinolyticus, Bacillus chondroitinus, Bacillus choshinensis, Bacillus cibi, Bacillus circulans, Bacillus clarkii, Bacillus clausii, Bacillus coagulans, Bacillus cobayae, Bacillus cohnii, Bacillus constellatus, Bacillus curdlanolyticus, Bacillus cycloheptanicus, Bacillus decolorationis, Bacillus dextrolacticus , Bacillus dipsosauri, Bacillus drentensis, Bacillus ehimensis, Bacillus endophyticus, Bacillus epiphytus, Bacillus farraginis, Bacillus fastidiosus, Bacillus filicolonicus, Bacillus finitimus, Bacillus firmus, Bacillus flavidus, Bacillus flavothermus, Bacillus flexus, Bacillus fordii, Bacillus formosus, Bacillus fortis, Bacillus funiculus, Bacillus fusiformis, Bacillus galactosidillyticus, Bacillus gelatini, Bacillus gibsonii, Bacillus globigii, Bacillus globisporus, Bacillus globisporus subsp. Marinus, Bacillus glucanolyticus, Bacillus gordonae, Bacillus halmapalus, Bacillus haloalkaliphilus, Bacillus halodenitrificans, Bacillus halodurans, Bacillus halophilus, Bacillus hollandicus, Bacillus horikoshii, Bacillus horti, Bacillus humi, Bacillus hwajinpoensis, Bacillus indicus, Bacillus infernos, Bacillus insolitus, Bacillus kaustophilus, Bacillus kobensis, Bacillus lactis niger 2, Bacillus laevolacticus, Bacillus larvae, Bacillus laterosporus, Bacillus lautus, Bacillus lentimorbus, Bacillus lentus, Bacillus licheniformis, Bacillus limnophilus, Bacillus lornisii, Bacillus longisporus, Bacillus longissimus, Bacillus macerans, Bacillus macquariensis, Bacillus macyae , Bacillus marinus, Bacillus marisflavi, Bacillus marismortui, Bacillus maroccanus, Bacillus medusa, Bacillus megaterium, Bacillus megaterium var phosphaticum, Bacillus megaterium / cereus, Bacillus mesentericus, Bacillus migulanus, Bacillus mojavensis, Bacillus muralis, Bacillus musculi, Bacillus mycoides, Bacillus my cours roseus II, Bacillus naganoensis, Bacillus natto, Bacillus nealsonii, Bacillus neidei, Bacillus niacini, Bacillus nigrificans, Bacillus nitritollens, Bacillus novalis, Bacillus okuhidensis, Bacillus oleronius, Bacillus pabuli, Bacillus pallidus, Bacillus palustris var. gelaticus, Bacillus pantothenticus, Bacillus para-alvei, Bacillus parabrevis, Bacillus pasteurii, Bacillus patagoniensis, Bacillus peoriae, Bacillus polymyxa, Bacillus pseudalcaliphilus, Bacillus pseudofirmus, Bacillus pseudogordonae, Bacillus pseudomycoides, Bacillus psychrodurans, Bacillus psychrophilus, Bacillus psychro saccharolyticus, Bacillus psychrotolerans, Bacillus pulvifaciens, Bacillus pumilus, Bacillus pycnoticus, Bacillus pycnus, Bacillus racemilacticus, Bacillus reuszeri, Bacillus salexigens, Bacillus saliphilus, Bacillus schlegelii, Bacillus selenitireducens, Bacillus silvestris, Bacillus similibadius, Bacillus simplex, Bacillus siralis, Bacillus smithii, Bacillus soli, Bacillus sonorensis, Bacillus sp., Bacillus sphaericus, Bacillus sphaericus subsp. Calidolactis, Bacillus subterraneus, Bacillus subtilis, Bacillus subtilis var. Aterrimus, Bacillus subtilis var. Niger, Bacillus subtilis subsp. Niger, Bacillus subtilis subsp. Spizizenii, Bacillus subtilis subsp. Subtilis, Bacillus teres, Bacillus terminalis, Bacillus thermantarcticus, Bacillus thermoacidurans, Bacillus thermoaegiptius, Bacillus thermoaerophilus, Bacillus thermoalcalophilus, Bacillus thermoamylovorans, Bacillus thermocatenulatus, Bacillus thermocloacae, Bacillus thermodenitrificans, Bacillus thermoglucosidasius, Bacillus thermoglucosidius, Bacillus thermoleovorans, Bacillus thermoruber, Bacillus thermosphaericus, Bacillus thermoterrestis, Bacillus thiaminolyticus, Bacillus thuringiensis, Bacillus thuringiensis subsp. aizawai., Bacillus thuringiensis subsp. alesti, Bacillus thuringiensis subsp. canadensis, Bacillus thuringiensis subsp. colmeri, Bacillus thuringiensis subsp. dakota, Bacillus thuringiensis subsp. darmstadiensis, Bacillus thuringiensis subsp. dendrolimus, Bacillus thuringiensis subsp. entomocidus, Bacillus thuringiensis subsp. finitimus, Bacillus thuringiensis subsp. galleriae, Bacillus thuringiensis subsp. Indiana, Bacillus thuringiensis subsp. israelensis, Bacillus thuringiensis subsp. kenyae, Bacillus thuringiensis subsp. kumamotoensis, Bacillus thuringiensis subsp. kurstaki, Bacillus thuringiensis subsp. kyushuensis, Bacillus thuringiensis subsp. morrisoni, Bacillus thuringiensis subsp. nigeriae, Bacillus thuringiensis subsp. ostriniae, Bacillus thuringiensis subsp. pakistani, Bacillus thuringiensis subsp. pondicheriensis, Bacillus thuringiensis subsp. shandongiensis, Bacillus thuringiensis subsp. sotto, Bacillus thuringiensis subsp. subtoxicus, Bacillus thuringiensis subsp. thuringiensis, Bacillus thuringiensis subsp. tochigiensis, Bacillus thuringiensis subsp. tohokuensis, Bacillus thuringiensis subsp. Tolworthi, Bacillus thuringiensis subsp. toumanoffi, Bacillus thuringiensis subsp. wuhanensis, Bacillus thuringiensis subsp. yunnanensis, Bacillus tusciae, Bacillus validus, Bacillus vallismortis, Bacillus vedderi, Bacillus vesiculiferous, Bacillus vireti, Bacillus viscosus, Bacillus vulcani, Bacillus weihenstephanensis, Bacillus xerothermodurans, Clostridium absonum, Clostridium aceticum, Clostridium acetireducens, Clostridium acetobutylicum, Clostridium acetonigenum, Clostridium acidisoli, Clostridium acidurici, Clostridium aerotolerans, Clostridium akagii, aldrichii Clostridium, Clostridium algidicarnis, algidixylanolyticum Clostridium, aminobutyricum Clostridium, aminophilum Clostridium, aminovalericum Clostridium, amygdalinum Clostridium, aurantibutyricum Clostridium autoethanogenum Clostridium baratii Clostridium, barkeri Clostridium, bartlettii Clostridium, Clostridium beijerinckii, Clostridium bifermentans , Clostridium bolteae, Clostridium bowmanii, Clostridium bryantii, Clostridium butylicum, Clostridium butyricum, Clostridium cadaveris, Clostridium caliptrosporum, Clostridium caminithermale, Clo stridium caproicum, Clostridium carnis, Clostridium celatum, Clostridium celerecrescens, Clostridium cellobioparum, Clostridium cellulolyticum, Clostridium cellulovorans, Clostridium chartatabidum, Clostridium chauvoei, Clostridium clostridioforme, Clostridium coccoides, Clostridium cochlearium, Clostridium cocleatum, Clostridium coli canis, Clostridium colinum, Clostridium collagenovorans, Clostridium corinoforum , Clostridium cylindrosporum, Clostridium difficile, Clostridium diolis, Clostridium disporicum, Clostridium durum, Clostridium estertheticum subsp. estertheticum, Clostridium estertheticum subsp. laramiense, Clostridium fallax, Clostridium favososporum, Clostridium felsineum, Clostridium fervidus, Clostridium filamentosum, Clostridium fimetarium, Clostridium flavum. Clostridium formicaceticum, frigidicarnis Clostridium, Clostridium frigoris, Clostridium gasigenes, Clostridium ghoni, ghonii Clostridium, glycolicum Clostridium, grantii Clostridium, Clostridium haemolyticum, Clostridium halophilum, hastiforme Clostridium, hathewayi Clostridium, Clostridium herbivorans, Clostridium hiranonis, histolyticum Clostridium, homopropionicum Clostridium, Clostridium hungatei , Clostridium hydroxybenzoicum, Clostridium hylemonae, Clostridium indolis, Clostridium innocuum, Clostridium intestinal, irregulare Clostridium, jejuense Clostridium isatidis, Clostridium, Clostridium kainantoi, Clostridium kluyveri, lactifermentum Clostridium lactatifermentans, Clostridium, lacusfryxellense Clostridium, laniganii Clostridium, Clostridium Laramie, laramiense Clostridium, Clostridium lentocellum, Clostridium lentoputrescens, Clostridium leptum, Clostridium limosum, Clostridium litorale, Clostridium lituseburense, Clostridium ljungdahlii, Clostridium lortetii, Clostridium magnum, Clostridium malenominatum, Clostridium mangenotii, Clostridium mayombei, Clostridium methoxybenzovorans, Clostridium methylpentosum, neopropionicum Clostridium, Clostridium nexile, Clostridium nigrificans, Clostridium novyi, Clostridium oceanicum, Clostridium oncolyticum, Clostridium orbiscindens, oroticum Clostridium, Clostridium oxalicum, Clostridium papyrosolvens, paradoxum Clostridium, Clostridium paraperfringens, paraputrificum Clostridium pasteurianum Clostridium pascui, Clostridium, pectinolyticum Clostridium, Clostridium peptidivorans, Clostridium perenne, Clostridium perfringens, Clostridium pfennigii, Clostridium plagarum, polysaccharolyticum Clostridium, populeti Clostridium propionicum Clostridium, proteoclasticum Clostridium, proteolyticum Clostridium, psychrophilum Clostridium, Clostridium puniceum Clostridium purinilyticum, Clostridium putrefaciens, Clostridium putrifi cum, quercicolum Clostridium, Clostridium quinii, ramosum Clostridium, Clostridium rectum, Clostridium roseum, rubrum Clostridium, Clostridium saccharo-butyl-acetonicuxn-liquefaciens, saccharobutylicum Clostridium saccharolyticum Clostridium, Clostridium saccharoperbutylacetonicum, Clostridium sardiniense, Clostridium sartagoforme, Clostridium scatologenes, Clostridium scindens, Clostridium septicum, Clostridium sordellii, Clostridium sp., Clostridium sphenoides, Clostridium spiroforme, Clostridium sporogenes, Clostridium sporosphaeroides, Clostridium stercorarium subsp. Leptospartum, Clostridium stercorarium subsp. Stercorarium, Clostridium stercorarium subsp. Thermolacticum, Clostridium sticklandii, straminisolvens Clostridium subterminale Clostridium symbiosum Clostridium, Clostridium termitidis, Clostridium tertium, Clostridium tetani, tetanomorphum Clostridium, thermaceticum Clostridium, thermautotrophicum Clostridium, thermoalcaliphilum Clostridium thermoamylolyticum Clostridium, thermobutyricum Clostridium thermocellum Clostridium thermohydrosulfuricum Clostridium, thermolacticum Clostridium, Clostridium thermopalmarium, Clostridium thermosaccharolyticum, Clostridium thermosuccinogenes, Clostridium thermosulfurigenes, Clostridium thiosulfatireducens, tunisiense Clostridium, tyrobutyricum Clostridium, Clostridium uliginosum, ultunense Clostridium, Clostridium uzonii, Clostridium villosum, Clostridium vincentii, Clostridium viride, Clostridium welchii, xylanolyticum Clostridium and Clostridium xylanovorans.

Under from these bacterial strains selected, non-pathogenic, spore-forming bacteria are again particular those bacteria or spores which are selected are preferred from the group consisting of Bacillus amyloliquefans, Bacillus subtilis, Bacillus licheniformis, Bacillus pumilus and Bacillus megaterium.

It is still according to the invention preferred that as non-pathogenic, spore-forming bacteria, those used bacteria selected from the bacterial strains mentioned above (or spores of those bacteria) that do not grow to secrete inhibitory agents from pathogenic bacteria. With "the growth active substances inhibiting pathogenic bacteria "are preferably antibiotics meant. Through the use of bacteria, which are not antibiotics can increase the proliferation of germs that are resistant to antibiotics resistant, are largely avoided.

Also particularly preferred are those of the above-mentioned, non-pathogenic, spore-forming bacteria that only poorly multiply in milk. By "poorly propagating in milk" it is preferably understood that 12 hours after inoculation of pasteurized milk with 1 x 10 ⁴ bacteria per milliliter of milk at an incubation temperature of 37 ° C less than 1 x 10 ⁶ , more preferably less than 1 x 10 ⁵ and most preferably less than 5 x 10 ⁴ bacteria are contained in the milk.

When Skin care products can in the composition of the invention All skin-care agents should be included, usually in udder-dip preparations are included. Particularly preferred as skin care agents Care products selected selected from the group consisting of glycerol, propylene glycol, 18- [beta] -glycyrrhetinic acid Licorice root extract (Gylcyrrhiza glabra), Aescin in horse chestnut (Aesculus hippocastanum), Allantoin, aloe vera (containing mainly sugar, anthraquinone and minerals like zinc), amino acids such as alanine, arginine, serine, lysine, ammonium glycyrrhizate from licorice root extract, Apigenin from chamomile extract (Matricaria recutita), Arnica, in particular Arnica montana or Arnica chamissonis, Asiaticoside and Madecassoside in Centella asiatica extract, Avenaanthramide from oat extract (Avena sativa), avocadol, azulene from chamomile extract (Matricaria recutita), Biotin (vitamin H), bisabolol from chamomile extract (Matricaria recutita), Brown algae extract (Ascophyllum nodosum), chlorogenic acid in water extract of the Japanese honeysuckle (Lonicera japonica), coenzyme Q10, creatine, Dexpanthenol, disodium glycyrrhizate from licorice root extract, extract from the red algae (Asparagopsis armata), flavonoids, in particular from birch extract (Betula alba), vitexin in the extract of passionflower (Passiflora incarnata), flavonoids, vitexin in linden extract (Tilia platyphyllos), ginkgo flavonium glycosides and terpenes, lactones in ginkgo extract (Ginkgo biloba), ginsenosides in ginseng extract (Panax ginseng), Glycogen, grapefruit extract, witch hazel extract from virgin Witch hazel (Hamamelis virgiana), honey, isoflavone glycosides in clover extract (Trifolium pratense), St. John's wort extract from St. John's wort (Hypericum perforatum), jojoba oil, Lecithin, corn oil (Zea mays), evening primrose oil, Niacinamide, Oenotheine B in Extract of fireweed (Epilobium angustifolium), Oleuropein in Olive Extract (Olea europea), Phytocohesin (Sodium Beta Sitosterolsulfate), Plankton extract (tetraselmis suecica, spirulina and others), polyphenols, Catechins from the extract of grape seeds (Vitis vinifera), polyphenols, Green catechins Tea (Camellia sinensis), Marigold Extract (Calendula officinalis), Rosmarinic acid in Melissa extract (Melissa officinalis), sandorn oil, [beta] -glucans from oats (Avena sativa), stearylglycyrrhetinic acid (stearyl ester the 18- [beta] beta Glycyrrhetinsäure) Sterols, sitosterol in stinging nettle extract (Urtica dioca), sweet almond oil (Prunus dulcis), vitamin C and its esters, vitamin E and its esters, wheat germ oil zinc gluconate / magnesium aspartate / copper gluconate, and zinc sulfate or zinc oxide used.

The Skin care agents are preferred in the composition of the invention in an amount ranging from 0.1 to 25% by weight, especially preferably in an amount in a range from 0.5 to 20% by weight and most preferably in an amount ranging from 1 to 10% by weight, based in each case on the total weight of the composition, contain. When the composition of the invention predominantly based on the spores of non-pathogenic, spore-forming bacteria, so may the proportion of skin care product in the composition also in a range of up to 99.9 wt .-%, preferably up to 90% by weight, more preferably up to 75% by weight, and most preferably up to 50 wt .-% amount.

Farther can the composition of the invention a carrier in particular when the composition according to the invention mainly based on (vegetative) bacteria, as carriers preferably all that Professional known nutrient media can be used. Particularly preferred nutrient media are nutrient broth, Meat water, dextrose broth, serum broth, ascites broth, blood broth, or even high-viscosity nutrient media such as Gelatine. With regard to the exact composition of the above Nutrient media as well further as a carrier suitable nutrient solutions on "guide of Medical Microbiology ", Gustav Fischer publishing house, Stuttgart, 5th edition, 1974, as well as on the EP-A-1 312 371, which are hereby incorporated by reference and their disclosure regarding the exact composition of Culture Media for bacteria forms part of the disclosure of the present invention.

The Composition according to the invention can in addition to the bacteria or spores, the skin care products and the carriers also further additives known to the person skilled in the art, such as dyes, Preservatives, viscosity regulators, Antioxidants and the like included.

Farther it is inventively preferred that the composition at a temperature of 25 ° C a Brookfield viscosity (DIN 53019) of at least 1.1 mPas, preferably at least 10 mPa.s, about that more preferably of at least 100 mPas, and most preferably of at least 1,000 mPa · s having.

a Contribution to the solution The input mentioned tasks also provides a method of inhibition the growth of pathogenic bacteria on lactating mammals, wherein the lactating mammal with non-pathogenic, spore-forming bacteria and / or with theirs Spores is brought into contact.

When non-pathogenic, spore-forming bacteria that interact with the lactating mammals those bacteria are preferred, already mentioned in the context of the composition according to the invention have been described. It can the non-pathogenic, spore-forming bacteria together with a suitable carrier, preferably a nutrient medium, be brought into contact with the lactating animal, as excipients those carriers preferred are those already mentioned in the context of the composition according to the invention have been described. Contacting mammals with bacteria or spores is in this case preferably by dipping or Spraying. However, it is also conceivable, the bacteria and / or spores as dry matter with the mammal to bring into contact. For this, the bacteria are beneficial first in a suitable aqueous broth multiplied and the bacterial suspension thus obtained ßend, for example by freeze-drying, converted into a solid, as in EP-A-1 312,371. This solid can then be lactated with mammal be brought into contact.

According to one particular embodiment of the inventive method become the non-pathogenic, spore-forming bacteria or their Spores in the form of the composition according to the invention described above brought into contact with the lactating animal.

Farther it is preferred in connection with the inventive method that the teats, the udder or the claws, but especially preferred the teats, the lactating mammal with the non-pathogenic, spore-forming bacteria or their spores be brought into contact. As lactating mammals are especially cattle, Buffalo, Goats, sheep, horses or camels are preferred.

According to one particular embodiment of the inventive method becomes the lactating mammal, preferably the teats, the udder or the claws of a lactating mammal, particularly preferably a cow, even with a disinfectant brought into contact, bringing into contact with the disinfectant before or after contact with those with non-pathogenic, spore-forming bacteria and / or can take place with their spores.

As disinfecting agents it is possible to use all disinfecting agents known to the person skilled in the art, which are usually used to disinfect the teats of a lactating mammal or else to disinfect other parts of the body of lactating mammals, such as the udder or the claws. Particularly preferred disinfectants are selected from the group consisting of alcohols, aldehydes, antimicrobial acids, carboxylic acid esters, acid amides, phenols, phenol derivatives, diphenyls, diphenylalkanes, urea derivatives, oxygen, nitrogen acetates and formals, benzamidines, isothiazolines, phthalimide derivatives, pyridine derivatives, guanidines , Chlorhexidines, alkylamines, alkyldiamines, alkyltriamines, antimicrobial amphoteric compounds, quinolines, 1,2-dibromo-2,4-dicyanobutane, iodo-2-propynyl-butyl-carbamate, iodine, iodophores, chlorine dioxide, alkali hypochlorites, peroxides and peracids ,

Especially Preferably, the disinfectant is selected from the group consisting of polyvinylpyrrolidine-iodine, chlorhexidine, derivatives of chlorhexidine, Mixtures of lactic acid and hydrogen peroxide or mixtures thereof.

According to one embodiment the method according to the invention becomes the lactating mammal, preferably the teat of a bovine, first with the disinfectant and subsequently with the non-pathogenic, spore-forming bacteria or with their Spores brought into contact. According to one another embodiment the method according to the invention becomes the lactating mammal, preferably the teat of a bovine, first with the non-pathogenic, spore-forming bacteria or with their spores and then with brought into contact with the disinfectant.

If in the method according to the invention mainly spores can be used according to a particular embodiment the method according to the invention the lactating mammal, preferably the teat of a cow, also simultaneously with the disinfectant and the spores are brought into contact. In this case, that will lactating mammal, preferably the teat of a cow, advantageously with a composition containing the disinfectant and the Spores brought into contact.

The Inventive method can basically used before or after milking a lactating animal become.

The The present invention also relates to the use of non-pathogenic, spore-forming bacteria or their spores, preferably for production a composition for inhibiting the growth of pathogenic bacteria in or on, preferably on a lactating mammal, being as a lactating mammal Cattle, Buffalo, Goats, sheep, horses, camels or humans are preferred and cattle are particularly preferred.

When non-pathogenic, spore-forming bacteria or their spores those bacteria or spores that are already preferred initially described in connection with the composition according to the invention were. According to one particular embodiment the use according to the invention the non-pathogenic, spore-forming bacteria or their spores These are used to treat mastitis or Mortellaro. In this context, it is further preferred that the non-pathogenic, spore-forming bacteria or their spores in the form of an udder-dip preparation be used.

The The invention further relates to the use of non-pathogenic, spore-forming Bacteria or their spores, preferably for the preparation of a composition, to inhibit the growth of pathogenic bacteria on the surfaces of Treatment devices for lactating mammals.

When Treatment device come in particular milking devices, preferably robotic Milking devices, as described for example in WO-A-03/79772 are described. Such milking devices comprise a milking facility with at least two milking stalls, which are arranged on a movable platform. Continue to come as a treatment device in example from WO-A-04/61436 known devices for determining the health status of an animal into consideration. Other treatment devices include in particular teat cups, Udder and teat cleaning devices as well as pre- and post-dipping devices.

for Inhibition of the growth of pathogenic bacteria on the surfaces of The aforementioned treatment devices can first disinfect them be in which the surfaces with be brought into contact with a suitable disinfectant. Suitable for disinfecting the surfaces For example, a device as described in WO-A-04/34775 is described. Then you can the surface brought into contact with the non-pathogenic, spore-forming bacteria become. It is also conceivable that, as well as in the inventive method for inhibiting the growth of pathogenic bacteria on lactating mammals, the surface the treatment devices first with the bacteria or spores and subsequently is brought into contact with the disinfectant.

The present invention further relates to the use of non-pathogenic, spore-forming bacteria or their spores for inhibiting the growth of pathogenic bacteria on the surfaces of stalls for preferably lactating mammals, wherein these surfaces preferably to lying areas, claw baths, linkage or wall surfaces in the stables is. Again, as with the treatment devices, the surfaces may be disinfected by suitable disinfectants before or after contact with the non-pathogenic, spore-forming bacteria.

The The invention will now be explained in more detail by way of non-limiting examples:

EXAMPLE 1

Bacillus subtilis is incubated in a nutrient broth as described in EP-A-1 321 371 on page 9, lines 11-12 for 18 hours at 30 ° C until a cell density of about 1 x 10 ⁹ cells / ml was reached.

After this the teats of a cow were cleaned with 40% ethanol solution the contacting teats with the cell suspension.

EXAMPLE 2

The The cell suspension obtained in Example 1 was freeze-dried. Subsequently the resulting powder containing spores of Bacillus subtilis, taken in glycerol with. It became a composition according to the invention obtained in the form of a suspension.

Claims (17)

A composition for inhibiting growth pathogenic bacteria on the teats of a lactating mammal, comprising a skin care agent, non-pathogenic, spore-forming Bacteria and / or their spores and optionally a carrier. The composition of claim 1, wherein the composition includes at least 1 x 10 ⁶ non-pathogenic, spore-forming bacteria and / or spores of these bacteria per gram of the composition. The composition according to any one of claims 1 or 2, wherein the non-pathogenic, spore-forming bacteria are selected from the group consisting of Bacillus and Clostridium. The composition according to one of the preceding Agent, wherein the non-pathogenic, spore-forming bacteria are selected from the group consisting of Bacillus amyloliquefans, Bacillus subtilis, Bacillus licheniformis, Bacillus pumilus and Bacillus megaterium. The composition according to one of the preceding Claims, where the non-pathogenic bacteria are not growth. of pathogenic bacteria secrete inhibitory agents. Method for inhibiting growth of pathogenic bacteria on lactating mammals, wherein the lactating mammal with non-pathogenic, spore-forming bacteria and / or with their spores is brought into contact. The method of claim 6, wherein the lactating mammal is also contacted with a disinfectant and Contact the disinfectant before or after in contact with those with non-pathogenic, spore-forming Bacteria and / or can be done with their spores. The method of claim 7, wherein the teats of the lactating mammals with the disinfectant and the non-pathogenic, spore-forming bacteria and / or the spores are brought into contact. Method according to one of claims 6 to 8, wherein the non-pathogenic, selected spore-forming bacteria are from the group consisting of Bacillus and Clostridium. Method according to one of claims 6 to 9, wherein the non-pathogenic, selected spore-forming bacteria are from the group consisting of Bacillus amyloliquefans, Bacillus subtilis, Bacillus licheniformis, Bacillus pumilus and Bacillus megaterium. A method according to any one of claims 6 to 10, wherein the composition according to one of the claims 1 to 5 is used. A method according to any one of claims 6 to 11, wherein the mammal selected is from the group comprising cattle, buffaloes, goats, sheep, and horses Camels. Use of non-pathogenic, spore-forming bacteria and / or their spores to inhibit the growth of pathogenic bacteria in or on a lactating mammal. Use of non-pathogenic, spore-forming bacteria and / or their spores for inhibiting the growth of pathogenic bacteria on the surfaces of treatment devices for lactating mammals. Use of non-pathogenic, spore-forming bacteria and / or their spores to inhibit the growth of pathogenic bacteria on the upper areas of stables for mammals. Use according to claim 12 for the treatment of mastitis or Mortellaro. Use according to claim 16, wherein the non-pathogenic, spore-forming bacteria and / or their spores in the form of an udder-dip preparation be used.