[go: up one dir, main page]

DE102004052707A1 - Sweat reducing cosmetic preparation - Google Patents

Sweat reducing cosmetic preparation Download PDF

Info

Publication number
DE102004052707A1
DE102004052707A1 DE102004052707A DE102004052707A DE102004052707A1 DE 102004052707 A1 DE102004052707 A1 DE 102004052707A1 DE 102004052707 A DE102004052707 A DE 102004052707A DE 102004052707 A DE102004052707 A DE 102004052707A DE 102004052707 A1 DE102004052707 A1 DE 102004052707A1
Authority
DE
Germany
Prior art keywords
sweat
nkcc1
glands
cosmetic preparation
sweat glands
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
DE102004052707A
Other languages
German (de)
Inventor
Thomas Schmidt-Rose
Lara Terstegen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Beiersdorf AG
Original Assignee
Beiersdorf AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Beiersdorf AG filed Critical Beiersdorf AG
Priority to DE102004052707A priority Critical patent/DE102004052707A1/en
Priority to EP05801499A priority patent/EP1804840A1/en
Priority to PCT/EP2005/055448 priority patent/WO2006042863A1/en
Priority to JP2007537284A priority patent/JP2008517039A/en
Publication of DE102004052707A1 publication Critical patent/DE102004052707A1/en
Withdrawn legal-status Critical Current

Links

Classifications

    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00Cosmetics or similar toiletry preparations
    • A61K8/18Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/46Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing sulfur
    • A61K8/466Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing sulfur containing sulfonic acid derivatives; Salts
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00Cosmetics or similar toiletry preparations
    • A61K8/18Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/49Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds
    • A61K8/4973Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with oxygen as the only hetero atom
    • A61K8/498Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with oxygen as the only hetero atom having 6-membered rings or their condensed derivatives, e.g. coumarin
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00Cosmetics or similar toiletry preparations
    • A61K8/18Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/67Vitamins
    • A61K8/673Vitamin B group
    • A61K8/675Vitamin B3 or vitamin B3 active, e.g. nicotinamide, nicotinic acid, nicotinyl aldehyde
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00Cosmetics or similar toiletry preparations
    • A61K8/18Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9783Angiosperms [Magnoliophyta]
    • A61K8/9789Magnoliopsida [dicotyledons]
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61QSPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q15/00Anti-perspirants or body deodorants

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • General Health & Medical Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Birds (AREA)
  • Epidemiology (AREA)
  • Mycology (AREA)
  • Microbiology (AREA)
  • Botany (AREA)
  • Biotechnology (AREA)
  • Engineering & Computer Science (AREA)
  • Cosmetics (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

Verwendung von Modulatoren und/oder Inhibitoren NKCC1 zur Beeinflussung des zellulären Ionentransportes.Use of modulators and / or inhibitors NKCC1 for influencing the cellular ion transport.

Description

Als klassische Antitranspirantien werden vor allem Aluminiumsalze oder Aluminium-/Zirkonium-Salze verwendet. Diese hemmen den Schweißfluss durch Verstopfung der Ausführungsgänge der Schweißdrüsen. Daher kann es zu einem Stau des Schweisses innerhalb der Drüse kommen. Darüber hinaus können solche Aluminiumsalze wie Aluminiumhydroxychloride bei häufiger Anwendung und empfindlichen Personen Hautschäden hervor rufen. Darüber hinaus kann es durch den Einsatz der Aluminiumsalze zu Verfärbungen von Textilien kommen, die mit dem Antitranspirans in Kontakt kommen.When classic antiperspirants are mainly aluminum salts or Aluminum / zirconium salts used. These inhibit the flow of sweat by obstructing the Excretory ducts of the sweat glands. Therefore There may be a congestion of sweat within the gland. Furthermore can such aluminum salts as Aluminiumhydroxychloride with frequent use and sensitive persons cause skin damage. Furthermore It may cause discoloration through the use of aluminum salts come from textiles that come into contact with the antiperspirant.

Zusätzlich zu den bislang eingesetzten Aluminium-/Zirkonium-Salzen sollen neue Wirkansätze gefunden werden, mit denen idealerweise der Schweiß effektiver und hautverträglicher unterdrückt werden kann. Hierbei ist das Ziel, nicht nur wie bisher den Schweißausfluss zu blockieren, sondern bereits die Bildung des Primärschweißes zu unterbinden.In addition to The aluminum / zirconium salts used so far are expected to be new Active approaches found which, ideally, make sweat more effective and more skin-friendly be suppressed can. Here is the goal, not just as before the sweat outflow to block, but already to prevent the formation of the primary sweat.

Der Mensch weist ca. 2 – 3 Mio. Schweißdrüsen am ganzen Körper auf. Ihre größte Dichte findet sich unter der Achsel und an Hand- und Fußflächen. Pro Tag werden 0,5 – max. 10 l Schweiß abgegeben.Of the Man has about 2 - 3 Million sweat glands all over body on. Your biggest density can be found under the armpit and on the palms and feet. Per day 0.5 - max. 10 l sweat.

Die Innervation der ekkrinen Schweißdrüse erfolgt ausgehend vom nervalen Stimulus über die peripheren vegetativen Nerven des Sympathikus. Die Signalübertragung von der Nerven- zur Schweißdrüsenzelle wird jedoch nicht wie üblich über Catecholamine sondern über den Neurotransmitter Acetylcholin (ACh) vermittelt (Sato et al., 1989). Die durch das entsprechende Aktionspotenzial ausgelöste Depolarisation der synaptischen Membran führt zur Öffnung Potenzial-abhängiger Kalzium-Kanäle. Der darauffolgende extrazelluläre Kalzium-Einstrom triggert die Exocytose der Acetylcholin-haltigen synaptischen Vesikel. Die daraufhin in den synaptischen Spalt ausgeschütteten Neurotransmitter binden an den G-Protein-gekoppelten muskarinischen Acetylcholinrezeptor der Schweißdrüsen-zelle (1). Das Signal wird über die G-Proteine auf die PLCβ übertragen, führt im weiteren zu einer Aktivierung der Proteinkinase C und zur Ausschüttung von intrazellulärem Kalzium aus den endoplasmatischen Kalzium-Speichern (2) (Quinton et al.). Aufgrund dieser zellulären Vorgänge kommt es zur Ausbildung eines Ionengradienten an dem im wesentlichen die Natrium/Kalium-ATPase, Natrium- und Chlorid-Kanäle sowie der Natrium-Kalium-Chlorid- Cotransporter (NKCC1) beteiligt sind (3). Das so entstehende osmotischen Gefälle führt zu einem Wassereinstrom von der basolateralen zur apikalen (luminalen) Seite der Zelle.The innervation of the eccrine sweat gland proceeds from the nervous stimulus via the peripheral vegetative nerves of the sympathetic. However, signaling from the nerve to the sweat gland cell is not mediated via catecholamines, as usual, but through the neurotransmitter acetylcholine (ACh) (Sato et al., 1989). The depolarization of the synaptic membrane triggered by the corresponding action potential leads to the opening of potential-dependent calcium channels. The subsequent extracellular calcium influx triggers the exocytosis of the acetylcholine-containing synaptic vesicles. The neurotransmitters released into the synaptic cleft bind to the G protein-coupled muscarinic acetylcholine receptor of the sweat gland cell ( 1 ). The signal is transferred via the G proteins to the PLC β, furthermore leads to an activation of the protein kinase C and to the release of intracellular calcium from the endoplasmic calcium stores ( 2 ) (Quinton et al.). As a result of these cellular processes, an ion gradient is formed in which essentially the sodium / potassium ATPase, sodium and chloride channels and the sodium-potassium chloride cotransporter (NKCC1) are involved ( 3 ). The resulting osmotic gradient leads to an influx of water from the basolateral to the apical (luminal) side of the cell.

Eine wesentliche Rolle beim Aufbau des Ionengradienten spielt der Na-K-Cl-Cotransporter (3).An essential role in the construction of the ion gradient plays the Na-K-Cl cotransporter ( 3 ).

Es hat sich nun überraschend und für den Fachmann nicht vorhersehbar herausgestellt, dass die Verwendung von Modulatoren und/oder Inhibitoren NKCC1 zur Beeinflussung des zelluären Ionentransportes den Nachteilen des Standes der Technik abhilft. Dabei ist es besonders bevorzugt, wenn Bumetanid oder Furosemid zur Beeinflussung des zelluären Ionentransportes eingesetzt wird. Weiter ist es besonders bevorzugt, wenn Sojaextrakt zur Inhibition der Schweißbildung von Schweißdrüsen, insbesondere menschlichen Schweißdrüsen verwendet wird.It has now become surprising and for the expert unpredictably pointed out that the use of modulators and / or inhibitors NKCC1 for influencing the zelluären Ion transport helps the disadvantages of the prior art. It is particularly preferred if Bumetanide or furosemide for influencing the cellular Ion transport is used. Furthermore, it is particularly preferred if soy extract to inhibit the sweat formation of sweat glands, in particular used human sweat glands becomes.

Eine Hemmung des Cotransporters unterbindet den Aufbau des Ionengradienten und des damit verbunden osmotischen Gefälles. Als Folge dessen erfolgt ein deutlich reduzierter Wassereinstrom von der basolateralen Seite der Zelle zum Lumen der Schweißdrüse hin. Analoge Vorgänge spielen sich auch in der Niere ab. Hier kann durch Blockierung des dort vorkommenden Na-K-Cl-Cotransporters (NKCC2) durch z.B. Bumetanid der Wassertransport inhibiert werden, was in Form von Diuretika auch therapeutisch getan wird. Die Inhibitoren bzw. Modulatoren des Na-K-Cl-Cotransporter 1, wie z.B. Bumetanid und Furosemid, sind für den kosmetischen Einsatz als Antitranspirantien geeignet.A Inhibition of the cotransporter prevents the formation of the ion gradient and the associated osmotic gradient. As a result of this occurs a significantly reduced influx of water from the basolateral side the cell to the lumen of the sweat gland. Analogous processes also take place in the kidney. Here can by blocking the occurring there Na-K-Cl cotransporter (NKCC2) by e.g. bumetanide the water transport can be inhibited, resulting in the form of diuretics is also done therapeutically. The inhibitors or modulators Na-K-Cl cotransporter 1, e.g. Bumetanide and Furosemide, are for the cosmetic use suitable as antiperspirants.

Darüber hinaus wurde folgendes herausgefunden:
In den Schweißzellen hemmt Bumetanid die Schweißsekretion.
Bumetanid beeinflußt die NaCl-Sekretion in den Sekretdrüsen.
Strukturen, die das NKCC1 beeinflussen, können zur Behandlung einer übermäßigen Schweißabsonderung an den Handflächen (Hyperhidrose) eingesetzt werden.
In addition, the following was found:
In the sweat cells, bumetanide inhibits sweat secretion.
Bumetanide affects NaCl secretion in the secretory glands.
Structures that affect NKCC1 can be used to treat excessive perspiration on the palms (hyperhidrosis).

Immunhistochemische und immunelektronenmikroskopische Lokalisierung von NKCC1Immunohistochemical and immunoelectron microscopic localization of NKCC1

Die immunhistochemische Analyse zeigte eine starke NKCC1-Markierung der basal-lateralen Plasmamembranbereiche der Sekretdrüsen von Ratten und der menschlichen Schweißdrüsen, jedoch keine Markierung des apikalen Plasmamembranbereichs. Im Vergleich zu den Sekretdrüsen war die Markierung im Reabsorptionsgang signifikant reduziert. Anti-NKCC1-Antikörper haben die Epidermis nicht markiert. Ein identisches Markierungsmuster wurde in den Schweißdrüsen der Maus gefunden. Anti-NKCC2-Antikörper haben Schweißdrüsen nicht markiert, aber Kontrollnierenschnitte. Wir konnten das NKCC1 örtlich auf die basal-laterale Plasmamembran der Sekretzellen von Schweißdrüsen beschränken. Das weist darauf hin, dass ein NKCC-Protein an der Schweißbildung sowie an der Abheilung der Schrumpfung, die man in ekkrinen Klarzellen nach Schweißeinleitung beobachtet, beteiligt ist.Immunohistochemical analysis revealed strong NKCC1 labeling of the basal-lateral plasma membrane areas of the rat gland and human sweat glands, but no labeling of the apical plasma membrane area. Compared to the secretory glands, the labeling was significantly reduced in the Reabsorptionsgang. Anti-NKCC1 antibodies did not label the epidermis. An identical labeling pattern was found in the sweat glands of the mouse. Anti-NKCC2 antibodies did not label sweat glands, but control kidney cuts. We localized the NKCC1 locally to the basal-lateral plasma restrict the membrane of the secrets of sweat glands. This suggests that an NKCC protein is involved in sweat formation as well as healing of shrinkage observed in eccrine clear cells after sweat introduction.

Damit bestätigt diese Arbeit die Hypothese und erbringt den physiologischen Nachweis für eine NKCC1-Lokalisierung auf den sekretorischen Teil der Schweißdrüsen sowie für die Beteiligung von NKCC1 an der Absonderung von isotonischer Flüssigkeit. Es ist sehr wahrscheinlich, dass NKCC1 an der Cl--Akkumulation in den Drüsenzellen gegen den elektrochemischen Gradienten beteiligt ist, und diese Akkumulation wiederum kann mit der Bildung des Gradienten für Cl- zu tun haben und somit mit der Na+/H2O-Sekretion in den Schweiß wie auch in anderen exokrinen Drüsen.Thus, this work confirms the hypothesis and provides the physiological evidence for NKCC1 localization to the secretory portion of the sweat glands and for the involvement of NKCC1 in the secretion of isotonic fluid. It is very likely that NKCC1 is involved in Cl - accumulation in the glandular cells against the electrochemical gradient, and this accumulation, in turn, may have to do with the formation of the gradient for Cl - and thus with the Na + / H 2 O- Secretion in the sweat as well as in other exocrine glands.

Schlussfolgernd konnten wir feststellen, dass NKCC1 in der basal-lateralen Plasmamembran von menschlichen Sekretdrüsen vorkommt, wo es wahrscheinlich für die Bumetanid-sensitive Sekretion von NaCl verantwortlich ist.In conclusion we could conclude that NKCC1 is present in the basal-lateral plasma membrane of human secretory glands occurs where it is likely for the bumetanide-sensitive secretion of NaCl is responsible.

Literaturliterature

  • Quinton, P. M. E., H. Y.; Mc Ewan Jenkinson, D.; Bovell, D. L. Structure and Function of Human Sweat Glands. In Antiperspirants and Deodorants, pp. 17-51Quinton, P.M.E., H.Y .; Mc Ewan Jenkinson, D .; Bovell, D.L. Structure and Function of Human Sweat Glands. In antiperspirants and deodorants, pp. 17-51
  • Sato, K., Kang, W. H., Saga, K., and Sato, K. T. (1989). Biology of sweat glands and their disorders. I. Normal sweat gland function, J Am Acad Dermatol 20, 537-563Sato, K., Kang, W.H., Saga, K., and Sato, K.T. (1989). Biology of sweat glands and their disorders. I. Normal sweat gland function, J Am Acad Dermatol 20, 537-563

Claims (3)

Verwendung von Modulatoren und/oder Inhibitoren NKCC1 zur Beeinflussung des zelluären Ionentransportes.Use of modulators and / or inhibitors NKCC1 for influencing the cellular ion transport. Verwendung nach Patentanspruch 1 dadurch gekennzeichnet, dass Bumetanid oder Furosemid zur Beeinflussung des zelluären Ionentransportes eingesetzt wird.Use according to claim 1, characterized that bumetanide or furosemide is used to influence the cellular ion transport becomes. Verwendung von Sojaextrakt zur Inhibition der Schweißbildung von Schweißdrüsen, insbesondere menschlichen Schweißdrüsen.Use of soy extract to inhibit perspiration of sweat glands, especially human Sweat glands.
DE102004052707A 2004-10-22 2004-10-22 Sweat reducing cosmetic preparation Withdrawn DE102004052707A1 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
DE102004052707A DE102004052707A1 (en) 2004-10-22 2004-10-22 Sweat reducing cosmetic preparation
EP05801499A EP1804840A1 (en) 2004-10-22 2005-10-21 Sweat-reducing cosmetic preparation
PCT/EP2005/055448 WO2006042863A1 (en) 2004-10-22 2005-10-21 Sweat-reducing cosmetic preparation
JP2007537284A JP2008517039A (en) 2004-10-22 2005-10-21 Antiperspirant cosmetic formulation

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE102004052707A DE102004052707A1 (en) 2004-10-22 2004-10-22 Sweat reducing cosmetic preparation

Publications (1)

Publication Number Publication Date
DE102004052707A1 true DE102004052707A1 (en) 2006-04-27

Family

ID=35502618

Family Applications (1)

Application Number Title Priority Date Filing Date
DE102004052707A Withdrawn DE102004052707A1 (en) 2004-10-22 2004-10-22 Sweat reducing cosmetic preparation

Country Status (4)

Country Link
EP (1) EP1804840A1 (en)
JP (1) JP2008517039A (en)
DE (1) DE102004052707A1 (en)
WO (1) WO2006042863A1 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2022258385A2 (en) 2021-06-07 2022-12-15 Unilever Ip Holdings B.V. Compositions and methods for controlling sweat production

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5019375A (en) * 1989-03-14 1991-05-28 The Procter & Gamble Company Low residue antiperspirant creams
US6309657B2 (en) * 1999-02-12 2001-10-30 The Procter & Gamble Company Cosmetic compositions
JP3661981B2 (en) * 1999-06-09 2005-06-22 株式会社ノエビア Antiperspirant
JP4033605B2 (en) * 2000-06-19 2008-01-16 ライオン株式会社 Aerosol antiperspirant composition
US20060088537A1 (en) * 2002-04-11 2006-04-27 Terrett Jonathan A Protein involved in cancer
DE10260954A1 (en) * 2002-12-20 2004-07-01 Henkel Kgaa Arylsulfatase inhibitors in deodorants and antiperspirants
DE60309027T2 (en) * 2003-08-26 2007-05-10 Johnson & Johnson Consumer France Sas Stabilized antiperspirant agent containing soy products
DE102004006829A1 (en) * 2004-02-09 2005-08-25 Beiersdorf Ag Cosmetic composition for e.g. preventing or treating acne or sebum production contains actives combination of isoflavones and antiperspirants

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2022258385A2 (en) 2021-06-07 2022-12-15 Unilever Ip Holdings B.V. Compositions and methods for controlling sweat production

Also Published As

Publication number Publication date
WO2006042863A1 (en) 2006-04-27
JP2008517039A (en) 2008-05-22
EP1804840A1 (en) 2007-07-11

Similar Documents

Publication Publication Date Title
DE69434115T2 (en) INHIBITOR OF THE GROWTH FACTOR FOR VITAL TREATMENT CELLS
EP0500605B1 (en) Cosmetic deodorants
KR101858095B1 (en) Cosmetic composition comprising Curcumae Longae Rhizoma extract comprising curcumin as an active ingredient for skin lightening, reducing wrinkle formation, and alleviating pruritus
DE1467921A1 (en) Process for the preparation of therapeutic or cosmetic agents for topical application
Olsson et al. The opioid receptor antagonist naloxone influences the pathophysiology of spinal cord injury
DE102004052707A1 (en) Sweat reducing cosmetic preparation
EP3463273A1 (en) Non-ionic surfactants for reducing fatty tissue
DE60026500T2 (en) POLYPEPTIDE FROM STRATUM CORNEUM AND ITS USE
WO2002100895A2 (en) Antimicrobially active peptide
JP2019530655A (en) Use of Arbutus unedo fruit extract as cosmetics
Baldwin The fine structure of healing over in mammalian cardiac muscle
DE60130802T2 (en) AGONISTS AND ANTAGONISTS OF UROTENSIN-II
Terlou et al. The development of a hypothalamic monoaminergic system for the regulation of the pars intermedia activity in Xenopus laevis
EP1169056A1 (en) Caspase inhibitors for inhibiting blood cell proliferation and for treating autoimmune diseases
DE60116478T2 (en) USE OF CDP-CHOLIN FOR THE PROPHYLAXIS OF CEREBRAL ISCHEMIA
Shen et al. Differential modulatory effects of α-and β-adrenoceptor agonists and antagonists on cortical immediate–early gene expression following focal cerebrocortical lesion-induced spreading depression
EP1054963B1 (en) Sequences of an ih ionic channel and the use thereof
WO2001027265A1 (en) Nucleic acid sequences of hyperplasia and tumours of the thyroid
WO2002017958A1 (en) Agent for influencing angiogenesis
Tsuda et al. Synergistic effects of Bay K 8644 and bradykinin on norepinephrine release in the hypothalamus of spontaneously hypertensive rats
Klee Einfluss der Angst sowie Niedergeschlagenheit im stationären Aufenthalt auf das klinische Outcome von Patienten mit osteoporotischer, thorakolumbaler Wirbelkörperfraktur
Beneit et al. Effects of oxytocin on the isolated vas deferens of the rat
Hattori et al. Morphological Estimation of Brain Extracellular Fluid Dynamics in Cold‐induced Edema from the Aspect of Cerebrospinal Fluid‐Extracellular Fluid Communication
DE102011052006A1 (en) A cosmetic composition containing a particular water and its use as a depigmenting or anti-aging active ingredient
Kurdow et al. Vergleich der Apoptoseinduktion durch Gemcitabine und durch Todes-Rezeptor (CD95)-Aktivierung beim bronchialen Plattenepithelzellkarzinom in vitro und in vivo

Legal Events

Date Code Title Description
R012 Request for examination validly filed

Effective date: 20110905

R120 Application withdrawn or ip right abandoned
R120 Application withdrawn or ip right abandoned

Effective date: 20130920