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DE102004048949A1 - Fish fodder, useful e.g. as feed additive in the pisciculture for the pigmentation of salmons and trout, comprises water soluble astaxanthin and solubilizer apart from proteins, lipids, carbohydrate, vitamins and minerals - Google Patents

Fish fodder, useful e.g. as feed additive in the pisciculture for the pigmentation of salmons and trout, comprises water soluble astaxanthin and solubilizer apart from proteins, lipids, carbohydrate, vitamins and minerals Download PDF

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Publication number
DE102004048949A1
DE102004048949A1 DE102004048949A DE102004048949A DE102004048949A1 DE 102004048949 A1 DE102004048949 A1 DE 102004048949A1 DE 102004048949 A DE102004048949 A DE 102004048949A DE 102004048949 A DE102004048949 A DE 102004048949A DE 102004048949 A1 DE102004048949 A1 DE 102004048949A1
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DE
Germany
Prior art keywords
astaxanthin
fish
pigmentation
trout
vitamins
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DE102004048949A
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German (de)
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Aquapharm Health & Nutrition G
Aquapharm Health & Nutrition GmbH
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Aquapharm Health & Nutrition G
Aquapharm Health & Nutrition GmbH
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Priority to DE102004048949A priority Critical patent/DE102004048949A1/en
Publication of DE102004048949A1 publication Critical patent/DE102004048949A1/en
Withdrawn legal-status Critical Current

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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K50/00Feeding-stuffs specially adapted for particular animals
    • A23K50/80Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/179Colouring agents, e.g. pigmenting or dyeing agents

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Polymers & Plastics (AREA)
  • Animal Husbandry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Insects & Arthropods (AREA)
  • Marine Sciences & Fisheries (AREA)
  • Birds (AREA)
  • Feed For Specific Animals (AREA)
  • Fodder In General (AREA)

Abstract

Fish fodder (I), for the pigmentation of salmons and trout in the fish farming, comprises water-soluble astaxanthin and additionally solubilizer with hydrophilic lipophilic balance (HLB) value of 9-18, e.g. polysorbate, apart from proteins, lipids, carbohydrate, vitamins and minerals. Independent claims are also included for: (1) a fish pigment comprising emulsifiers of E432, E435 and/or E433, where astaxanthin is dissolved in it; and (2) the preparation of (I).

Description

1. Anwendungsgebiet1. Application

Die Erfindung betrifft Fischfutter, in das stabiles, zur Pigmentierung von Lachsen und Forellen geeignetes wässriges Solubilisat von Astaxanthin mit einer Partikelgröße kleiner 50 nm, das mit Hilfe von Emulgatoren hergestellt wird, eingearbeitet wird.The The invention relates to fish feed, in the stable, for pigmentation Aqueous solubilisate of astaxanthin suitable for salmon and trout smaller with a particle size 50 nm, which is produced with the aid of emulsifiers incorporated becomes.

2. Stand der Technik2. Stand the technology

Fischfutter besteht im allgemeinen aus Proteinen, Lipiden, Kohlenhydraten, Vitaminen und Mineralstoffen.fish food generally consists of proteins, lipids, carbohydrates, vitamins and minerals.

Nach der EU-Richtlinie EU-RL 70/524/EWG, aktualisiert vom 26.2.2004, ist zur Pigmentierung von Lachsen und Forellen bei der Fischzucht eine Menge von Astaxanthin (E 161 j) bis 100 mg pro Kg Fischfutter zugelassen. Das fettlösliche Astaxanthin wird in sog. beadlets mikroverkapselt, die eine Partikelgröße von > 300 μm (0,3 mm) haben, dem Fischfutter beigemischt. Das Carotinoid Astaxanthin, das entweder synthetisch erzeugt oder vorwiegend aus Mikroalgen gewonnen wird, wird daneben auch als Wachstumsförderer, Photoprotektor, Immunstimulans und als Radikalfänger eingesetzt.To EU Directive 70/524 / EEC, updated on 26.2.2004, is for the pigmentation of salmon and trout in fish farming a quantity of astaxanthin (E 161 j) to 100 mg per kg of fish food authorized. The fat-soluble Astaxanthin is microencapsulated in so-called beadlets, which have a particle size of> 300 μm (0.3 mm) have added to the fish food. The carotenoid astaxanthin, either synthetically produced or obtained predominantly from microalgae becomes, besides, also as a growth promoter, photoprotector, immune stimulant and as radical scavengers used.

3. Nachteile des Standes der Technik3. Disadvantages of the prior art

In einer Stellungnahme vom 8. März 1989 stellt das wissenschaftliche Komitee der EU-Kommission fest, dass nur 10 % des im Fischfutter enthaltenen Astaxanthin absorbiert werden. Diese geringe Absorption (Bioverfügbarkeit) des fettlöslichen Astaxanthin ist deshalb von großer Bedeutung, weil Astaxanthin ca, 60 % der Futterkosten ausmacht. IM EFSA Journal (2004) 43, 1-4, stellt das Wissenschaftliche Gremium für Zusatzstoffe, Erzeugnisse und Stoffe in der Tierernährung über die Umweltauswirkungen von Astaxanthin reicher Phaffia rhodozyma als Futterzusatzstoff gemäß Richtlinie 70/524/EWG des Rates in einem Gutachten fest, dass nach einer von einem Hersteller vorgelegten Studie 50 % des Astaxanthin ausgeschieden werden. „Dabei kann nicht unterstellt werden, dass sein Verbleib und dessen Auswirkungen ohne jeglichen Einfluss auf die Umwelt bleiben". Es wurde beanstandet, dass jedoch keine Daten über den Verbleib und das Verhalten von Astaxanthin in Wasser (Löslichkeit) und Sediment vorgelegt wurden.In an opinion dated 8 March In 1989, the scientific committee of the EU Commission states that only 10% of the astaxanthin contained in the fish feed absorbs become. This low absorption (bioavailability) of the fat-soluble Astaxanthin is therefore of great Meaning, because astaxanthin makes up about 60% of the feed costs. In the EFSA Journal (2004) 43, 1-4, represents the Scientific Panel on Additives, Products and Substances used in animal nutrition via Environmental effects of astaxanthin rich Phaffia rhodozyma as Feed additive according to Directive 70/524 / EEC of the Council in an opinion that, according to one of a manufacturer 50% of astaxanthin are excreted. "It can not be assumed that his whereabouts and its effects without any impact on the environment. "However, it was objected no data about the fate and behavior of astaxanthin in water (solubility) and Sediment were submitted.

4. Aufgabe der Erfindung4th task the invention

Die Aufgabe dieser Erfindung ist es, Fischfutter mit einem wasserlöslichen Astaxanthin mit einer Parfikelgröße von < 50 nm anzureichern, da hierdurch die Bioverfügbarkeit stark erhöht werden kann.The The object of this invention is to fish food with a water-soluble To enrich astaxanthin with a particle size of <50 nm, because of this the bioavailability greatly increased can be.

Um Astaxanthin mit einer Partikelgröße von < 50 nm wasserlöslich zu machen, werden die Polysorbate 20 (E 432), 60 (E 435) bzw. 80 (E433) verwendet. Dieses wasserlösliche Astaxanthin wird zusammen mit den übrigen Wirkstoffen dem Fischfutter beigemischt. 1 zeigt den RMS-Radius (nm) verschiedener Proben von wasserlöslichem Astaxanthin.In order to make astaxanthin with a particle size of <50 nm water-soluble, the polysorbates 20 (E 432), 60 (E 435) and 80 (E433) are used. This water-soluble astaxanthin is added to the fish food together with the other active ingredients. 1 shows the RMS radius (nm) of various samples of water-soluble astaxanthin.

Studien zeigen, dass wasserlösliches Astaxanthin eine bessere Bioverfügbarkeit von 1,7 bis 3,7 fach hat (EUR J Pharm Scl. 2003 Jul; 10(4):299-304....studies show that water-soluble Astaxanthin for better bioavailability from 1.7 to 3.7 times (EUR J Pharm Scl. 2003 Jul; 10 (4): 299-304 ....

Überraschenderweise wurde gefunden, dass durch die bessere Bioverfügbarkeit das von dem, in Fischfutter verarbeiteten, wasserlöslichen Astaxanthin 40-70 % weniger eingesetzt werden kann (gemessen mit dem Chroma-Meter CR-300 von Minolta), um die gewünschte Pigmentierung bei der Fischzucht zu bewirken.Surprisingly It has been found that by the better bioavailability of that, in fish feed processed, water-soluble Astaxanthin 40-70% less can be used (measured with Minolta's chroma meter CR-300) to achieve the desired pigmentation at the Fish farming.

2 zeigt den Vergleich der Bioverfügbarkeit (Resorption) von fettlöslichem und wasserlöslichem Astaxanthin. 2 shows the comparison of the bioavailability (absorption) of fat-soluble and water-soluble astaxanthin.

Nachdem die Kosten von Astaxanthin ca. 60 % der Gesamtkosten des Fischfutter ausmachen, lassen sich die Herstellungskosten des Fischfutters um 25 – 40 % senken. Da weniger eingesetzt wird, wird auch weniger ausgeschieden und damit wird die Umweltbelastung deutlich reduziert.After this the cost of astaxanthin is approximately 60% of the total cost of the fish feed make up, the manufacturing costs of the fish feed can be 25 - 40 % reduce. As less is used, less is eliminated and thus the environmental impact is significantly reduced.

Claims (5)

Fischfutter, dadurch gekennzeichnet, dass es neben Proteinen, Lipiden, Kohlehydraten, Vitaminen und Mineralien zur Pigmentierung von Lachsen und Forellen in der Fischzucht wasserlösliches Astaxanthin und zusätzlich Lösungsvermittler mit einem HLB-Wert von ungefähr 9 bis ungeführ 18 enthält, z.B. Polysorbate.Fish food, characterized in that it is next to Proteins, lipids, carbohydrates, vitamins and minerals Pigmentation of salmon and trout in fish farming watersoluble Astaxanthin and additionally solubilizers with an HLB value of approximately 9 to approx Contains 18, e.g. Polysorbate. Fischfutter nach Anspruch 1 dadurch gekennzeichnet, dass der Anteil des wasserlöslichen Astaxanthin eine Menge von < 100 g/kg Fischfutter enthält.Fish food according to claim 1, characterized that the proportion of water-soluble Astaxanthin a lot of <100 g / kg fish feed. Fischfutter nach Anspruch 1 oder 2 dadurch gekennzeichnet, dass das darin enthaltene wasserlösliche Astaxanthin eine Partikelgröße von < 50 nm hat.Fish food according to claim 1 or 2, characterized that the water-soluble astaxanthin contained therein has a particle size of <50 nm. Fischpigment bestehend aus Emulgatoren E432, E435 und/oder E433 und darin gelöstem Astaxanthin.Fish pigment consisting of emulsifiers E432, E435 and / or E433 and dissolved therein Astaxanthin. Verfahren zur Herstellung von Fischfutter, dadurch gekennzeichnet, dass Astaxanthin in Polysorbaten gelöst dem Fischfutter zugesetzt wird.Process for the preparation of fish feed, characterized in that astaxanthin in polysor Baten solved the fish feed is added.
DE102004048949A 2004-10-07 2004-10-07 Fish fodder, useful e.g. as feed additive in the pisciculture for the pigmentation of salmons and trout, comprises water soluble astaxanthin and solubilizer apart from proteins, lipids, carbohydrate, vitamins and minerals Withdrawn DE102004048949A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
DE102004048949A DE102004048949A1 (en) 2004-10-07 2004-10-07 Fish fodder, useful e.g. as feed additive in the pisciculture for the pigmentation of salmons and trout, comprises water soluble astaxanthin and solubilizer apart from proteins, lipids, carbohydrate, vitamins and minerals

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DE102004048949A DE102004048949A1 (en) 2004-10-07 2004-10-07 Fish fodder, useful e.g. as feed additive in the pisciculture for the pigmentation of salmons and trout, comprises water soluble astaxanthin and solubilizer apart from proteins, lipids, carbohydrate, vitamins and minerals

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3117838B1 (en) 2014-03-10 2020-09-16 Saraya Co., Ltd. Composition containing sophorolipid, physiologically active substance and oil and fat, and method of producing said composition
EP4193845A1 (en) 2021-12-13 2023-06-14 OASE GmbH Fish feed composition and use thereof

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2411529A1 (en) * 1973-03-12 1974-09-26 Hoffmann La Roche COLOR PREPARATION
US4239782A (en) * 1979-04-16 1980-12-16 Aquatic Diet Technology Inc. Fish food composition and a process for enhancing the color of fish
DE4100739A1 (en) * 1991-01-12 1992-07-16 Basf Ag Feedstuff contg. neurospora-xanthine or its ester(s) - used to improve pigmentation of egg yolks, skin, meat and legs of broiler(s) and flesh of fish and crustacea and their shells
EP0628258A1 (en) * 1992-12-25 1994-12-14 Kanegafuchi Kagaku Kogyo Kabushiki Kaisha Granular colorant and formula feed containing the same
WO1997042838A1 (en) * 1996-05-15 1997-11-20 Ewos Limited Method for producing feed pellets
US6190686B1 (en) * 1996-01-22 2001-02-20 Chr. Hansen A/S Water dispersible compositions containing natural hydrophobic pigment, method of preparing same and their use
EP1176876B1 (en) * 1999-05-07 2004-01-14 Ewos Limited Pigment
WO2004016099A1 (en) * 2002-08-14 2004-02-26 Zoolife International Limited Composition for dietary enrichment

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2411529A1 (en) * 1973-03-12 1974-09-26 Hoffmann La Roche COLOR PREPARATION
US4239782A (en) * 1979-04-16 1980-12-16 Aquatic Diet Technology Inc. Fish food composition and a process for enhancing the color of fish
DE4100739A1 (en) * 1991-01-12 1992-07-16 Basf Ag Feedstuff contg. neurospora-xanthine or its ester(s) - used to improve pigmentation of egg yolks, skin, meat and legs of broiler(s) and flesh of fish and crustacea and their shells
EP0628258A1 (en) * 1992-12-25 1994-12-14 Kanegafuchi Kagaku Kogyo Kabushiki Kaisha Granular colorant and formula feed containing the same
US6190686B1 (en) * 1996-01-22 2001-02-20 Chr. Hansen A/S Water dispersible compositions containing natural hydrophobic pigment, method of preparing same and their use
WO1997042838A1 (en) * 1996-05-15 1997-11-20 Ewos Limited Method for producing feed pellets
EP1176876B1 (en) * 1999-05-07 2004-01-14 Ewos Limited Pigment
WO2004016099A1 (en) * 2002-08-14 2004-02-26 Zoolife International Limited Composition for dietary enrichment

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3117838B1 (en) 2014-03-10 2020-09-16 Saraya Co., Ltd. Composition containing sophorolipid, physiologically active substance and oil and fat, and method of producing said composition
EP4193845A1 (en) 2021-12-13 2023-06-14 OASE GmbH Fish feed composition and use thereof

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