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DE102004016247A1 - Solid support systems for homogeneous detection of interactions of biomolecules without washing steps - Google Patents

Solid support systems for homogeneous detection of interactions of biomolecules without washing steps Download PDF

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Publication number
DE102004016247A1
DE102004016247A1 DE200410016247 DE102004016247A DE102004016247A1 DE 102004016247 A1 DE102004016247 A1 DE 102004016247A1 DE 200410016247 DE200410016247 DE 200410016247 DE 102004016247 A DE102004016247 A DE 102004016247A DE 102004016247 A1 DE102004016247 A1 DE 102004016247A1
Authority
DE
Germany
Prior art keywords
biomolecules
decay time
washing steps
interactions
solid support
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
DE200410016247
Other languages
German (de)
Inventor
Otto S Wolfbeis
Petra Bastian
Michaela Gruber
Bianca Katherina Wetzll
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Chromeon GmbH
Original Assignee
Chromeon GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Chromeon GmbH filed Critical Chromeon GmbH
Priority to DE200410016247 priority Critical patent/DE102004016247A1/en
Priority to PCT/EP2005/003523 priority patent/WO2005095982A1/en
Publication of DE102004016247A1 publication Critical patent/DE102004016247A1/en
Withdrawn legal-status Critical Current

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

Abstract

Gegenstand der Erfindung sind feste Materialien, insbesondere Glas- oder Plastikplatten, multiwell Mikrotiterplatten, Mikroskopie-Träger, Teststreifen und andere, die mit Biomolekülen, insbesondere fluoreszent markierten, kovalent oder unkovalent beschickt sind. Diese ermöglichen es, komplementäre Biomoleküle über die Messung der Änderung der Fluoreszenzintensität, der Fluoreszenz-Abklingzeit ("Lifetime" oder "Decay time"), einem Energietransfer oder einer Löschung von Luminophoren direkt und ohne Waschschritte an der Grenzfläche nachzuweisen. Weiterhin ist Gegenstand der Erfindung ein Verfahren, das auf festen Materialien, beispielsweise Gips- oder Plastikplatten, Cellulosen, oder silikatischen Materialien, multiwell Mikrotiterplatten, Mikroskopie-Trägern, Teststreifen und anderen, beruht, welche mit Biomolekülen kovalent oder unkovalent beschickt wurden, und eine Wechselwirkung von Biomolekülen über eine Änderung der Fluoreszenz-Abklingzeit und eventuell über Änderungen der Fluoreszenzintensität anzeigt.The invention relates to solid materials, in particular glass or plastic plates, multiwell microtiter plates, microscopy carrier, test strips and others, which are charged with biomolecules, in particular fluorescently labeled, covalent or uncovalent. These make it possible to detect complementary biomolecules via the measurement of the change in the fluorescence intensity, the fluorescence decay time ("lifetime" or "decay time"), an energy transfer or a quenching of luminophores directly and without washing steps at the interface. The invention furthermore relates to a process based on solid materials, for example gypsum or plastic plates, celluloses, or siliceous materials, multiwell microtiter plates, microscope slides, test strips and others, which have been covalently or noncovalently charged with biomolecules, and an interaction of biomolecules indicating a change in fluorescence decay time and possibly changes in fluorescence intensity.

DE200410016247 2004-04-02 2004-04-02 Solid support systems for homogeneous detection of interactions of biomolecules without washing steps Withdrawn DE102004016247A1 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
DE200410016247 DE102004016247A1 (en) 2004-04-02 2004-04-02 Solid support systems for homogeneous detection of interactions of biomolecules without washing steps
PCT/EP2005/003523 WO2005095982A1 (en) 2004-04-02 2005-04-04 Solid support systems for the homogeneous detection of interactions of biomolecules without washing steps

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE200410016247 DE102004016247A1 (en) 2004-04-02 2004-04-02 Solid support systems for homogeneous detection of interactions of biomolecules without washing steps

Publications (1)

Publication Number Publication Date
DE102004016247A1 true DE102004016247A1 (en) 2005-10-20

Family

ID=34965491

Family Applications (1)

Application Number Title Priority Date Filing Date
DE200410016247 Withdrawn DE102004016247A1 (en) 2004-04-02 2004-04-02 Solid support systems for homogeneous detection of interactions of biomolecules without washing steps

Country Status (2)

Country Link
DE (1) DE102004016247A1 (en)
WO (1) WO2005095982A1 (en)

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9119735D0 (en) * 1991-09-16 1991-10-30 Secr Defence Gene probe biosensor method
JPH11164700A (en) * 1997-12-04 1999-06-22 Hitachi Ltd DNA detection method
US6207831B1 (en) * 1998-12-21 2001-03-27 Novartis Ag Fluorescent dyes (AIDA) for solid phase and solution phase screening
DE10038080A1 (en) * 2000-08-04 2002-02-21 Giesing Michael Registering the presence of immobilized substances on a bio-chip carrier, comprises using a fluorescence scanner, where a pulsed laser excites fluorescent markings to be detected between the pulses with local resolution
DE10133844B4 (en) * 2001-07-18 2006-08-17 Micronas Gmbh Method and device for detecting analytes
DE10153818A1 (en) * 2001-11-05 2003-05-15 Chromeon Gmbh Methods and compounds for fluorescent labeling of biomolecules and polymer particles
DE10157615A1 (en) * 2001-11-26 2003-06-05 Chromeon Gmbh New fluorochromes for labeling biomolecules and polymer particles, and the bioanalytical and screening processes based on them
DE10308814A1 (en) * 2003-02-27 2004-09-09 Chromeon Gmbh Bioanalytical method based on the measurement of the decay time of phosphorescence

Also Published As

Publication number Publication date
WO2005095982A1 (en) 2005-10-13

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