DE1068862B - Procedure for extending the shelf life of blood products - Google Patents
Procedure for extending the shelf life of blood productsInfo
- Publication number
- DE1068862B DE1068862B DENDAT1068862D DE1068862DA DE1068862B DE 1068862 B DE1068862 B DE 1068862B DE NDAT1068862 D DENDAT1068862 D DE NDAT1068862D DE 1068862D A DE1068862D A DE 1068862DA DE 1068862 B DE1068862 B DE 1068862B
- Authority
- DE
- Germany
- Prior art keywords
- blood
- shelf life
- extending
- blood products
- procedure
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000000034 method Methods 0.000 title claims description 6
- 239000010836 blood and blood product Substances 0.000 title description 6
- 229940125691 blood product Drugs 0.000 title description 6
- 210000004369 blood Anatomy 0.000 claims description 16
- 239000008280 blood Substances 0.000 claims description 16
- 229940099898 chlorophyllin Drugs 0.000 claims description 6
- 235000019805 chlorophyllin Nutrition 0.000 claims description 6
- 150000001447 alkali salts Chemical class 0.000 claims description 5
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 3
- JVOGSHDZLOJKKR-MXFMKSRJSA-I [Na+].[Na+].[Na+].[Mg++].CCc1c(C)c2cc3[n-]c(c(C)c3C=C)c(C)c3nc(C[C@H]3CCC([O-])=O)c(CC([O-])=O)c3[n-]c(cc1n2)c(C)c3C([O-])=O Chemical class [Na+].[Na+].[Na+].[Mg++].CCc1c(C)c2cc3[n-]c(c(C)c3C=C)c(C)c3nc(C[C@H]3CCC([O-])=O)c(CC([O-])=O)c3[n-]c(cc1n2)c(C)c3C([O-])=O JVOGSHDZLOJKKR-MXFMKSRJSA-I 0.000 claims description 2
- 239000000654 additive Substances 0.000 claims 1
- 230000000996 additive effect Effects 0.000 claims 1
- 230000023597 hemostasis Effects 0.000 claims 1
- 210000002381 plasma Anatomy 0.000 description 5
- 239000000243 solution Substances 0.000 description 5
- 210000003743 erythrocyte Anatomy 0.000 description 4
- 239000003381 stabilizer Substances 0.000 description 3
- 230000035602 clotting Effects 0.000 description 2
- 230000002949 hemolytic effect Effects 0.000 description 2
- 239000001509 sodium citrate Substances 0.000 description 2
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 2
- RZVAJINKPMORJF-UHFFFAOYSA-N Acetaminophen Chemical compound CC(=O)NC1=CC=C(O)C=C1 RZVAJINKPMORJF-UHFFFAOYSA-N 0.000 description 1
- 206010053567 Coagulopathies Diseases 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 206010018910 Haemolysis Diseases 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 230000036770 blood supply Effects 0.000 description 1
- 230000000711 cancerogenic effect Effects 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 239000000975 dye Substances 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 230000008588 hemolysis Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 229930014626 natural product Natural products 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 239000001044 red dye Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/14—Blood; Artificial blood
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/10—Preservation of living parts
- A01N1/12—Chemical aspects of preservation
- A01N1/122—Preservation or perfusion media
- A01N1/126—Physiologically active agents, e.g. antioxidants or nutrients
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Zoology (AREA)
- Dentistry (AREA)
- Wood Science & Technology (AREA)
- Environmental Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Hematology (AREA)
- Immunology (AREA)
- Virology (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Description
Verfahren zur Verlängerung der Haltbarkeit von Blutkonserven Die Erfindung betrifft ein Verfahren zur Verlängerung der Haltbarkeit von Blutkonserven.Method for extending the shelf life of blood products The invention relates to a method for extending the shelf life of blood products.
Bekanntlich bestehen Blutkonserven aus Aufschwemmungen von größtenteils 4 Teilen Frischblut mit 1 Teil einer Stabilisatorlösung, die das Blut vor Gerinnung und Ger innselbildung schützen soll. Als Stabilisator wird am häufigsten Natrium-Zitrat mit einem Zusatz von Traubenzucker in wäßriger Lösung benutzt.It is well known that blood supplies consist of mostly suspensions 4 parts of fresh blood with 1 part of a stabilizer solution that prevents the blood from clotting and should protect clot formation. Sodium citrate is the most common stabilizer used with an addition of glucose in an aqueous solution.
In dieser Weise hergestellte und bei etwa -l-4° C aufbewahrte Blutkonserven weisen nach 14 Tagen eine zunehmende Rotfärbung im darüberstehenden, normalerweise zartgelben Plasmaanteil auf. Dieser als Hämolyse bezeichnete Vorgang kommt durch Platzen der roten Blutkörperchen zustande, deren roter Farbstoff dann in das Blutplasma übertritt. Von hämolytischen Blutkonserven wird üblicherweise kein Gebrauch mehr gemacht. Nach C u c c i o 1 i , U., Arch. ist. biochim. ital., 14, 189 (1942), treten die ersten hämolytischen Zeichen in Blutkonserven vom 15. Tage ab auf, so daß das Blut von diesem Zeitpunkt ab nicht mehr zu Transfusionen zu benutzen ist. S chürch, O., Acta soc. medic. Fenn. duodezim., B 31, 58 (1941), dagegen gibt 3 Wochen als höchste Verwendungsdauer von Blutkonserven an. Diese beschränkte Verwendungsdauer ist bei dem ständig steigenden Bedarf von erheblichem Nachteil, auch wenn man bei innerhalb dieser Zeit nicht verbrauchten Blutkonserven versucht, das über den roten Blutkörperchen stehende Blutplasma einer weiteren Verwendung zuzuführen, indem man es abpipettiert und in dieser Form zu sogenannten Plasmatransfusionen benutzt. In diesem fehlen jedoch die roten Blutkörperchen, so daß Plasmatransfusionen kein voller Ersatz der Vollbluttransfusion sein können. Neben dem Funktionsverlust ist auch die volumetrische Abnahme der Transfusionsflüssigkeit beträchtlich, da mit den Erythrocyten etwa ein Drittel bis die Hälfte des Volumens einer Blutkonserve verlorengeht.Consists of blood produced in this way and stored at around -1-4 ° C show after 14 days an increasing red color in the upper part, usually pale yellow plasma portion. This process known as hemolysis comes through The red blood cells burst, their red dye then enters the blood plasma transgresses. Hemolytic blood reserves are usually no longer used made. According to C u c c i o 1 i, U., Arch. Is. biochim. ital., 14, 189 (1942) the first haemolytic signs in blood products from the 15th day onwards, so that the Blood can no longer be used for transfusions from this point onwards. S chürch, O., Acta soc. medic. Fenn. duodezim., B 31, 58 (1941), on the other hand there are 3 weeks as maximum usage time of blood products. This limited period of use is at a considerable disadvantage with the constantly increasing demand, even if you are at within this time unused blood products tried that over the red Blood cells to supply standing blood plasma for further use by it is pipetted off and used in this form for so-called plasma transfusions. In However, this lacks the red blood cells, so that plasma transfusions do not have a full Replacement of whole blood transfusion. In addition to the loss of function is also the volumetric decrease of the transfusion liquid is considerable, since with the erythrocytes about a third to half the volume of a blood unit is lost.
Obwohl man durch Variationen des Stabilisators versucht hat, die Konservierungs- bzw. Verwendungsdauer menschlichen Blutes zu verlängern, ist es bis jetzt nicht gelungen, die maximale Grenze von 3 Wochen zu überschreiten.Although attempts have been made to reduce the preservation properties by varying the stabilizer To date, it has not been done to extend or extend the useful life of human blood managed to exceed the maximum limit of 3 weeks.
Die geschilderten Mängel werden durch die Erfindung behoben, indem dem Blut unmittelbar nach der Entnahme, d. h. dem Frischblut, Alkalisalze des Chlorophyllins in isotonischer Kochsalzlösung in einer Konzentration von etwa 0,0001 bis 2,011/o zugesetzt werden. Die so vorbehandelte Blutkonserve ist etwa zwei- bis dreimal so lange haltbar wie bisher. Selbstverständlich muß die Chlorophyllin-Verbindung bzw. die aus den Alkalisalzen des Chlorophyllins hergestellte Lösung steril filtriert und frei von Schwebestoffen sein. So kann man beispielsweise zu einer Menge von 100 ccm Frischblut, welches in bekannter Weise durch den Zusatz von 20 ccm einer 3,8%-igen Natrium-Citrat-Lösung ungerinnbar gemacht ist, ein Alkalisalz des Chlorophyllins in isotonischer Kochsalzlösung in einer Konzentration von 2% hinzufügen. Die verwendeten Alkalisalze des Chlorophyllins, wie beispielsweise Chlorophyllin-Natrium, sind ein praktisch atoxisches Naturprodukt, dem auch keine carcinogene Wirkung wie manchen anderen Farbstoffen zukommt.The deficiencies outlined are remedied by the invention by the blood immediately after collection, d. H. the fresh blood, alkali salts of chlorophyllin in isotonic saline solution at a concentration of about 0.0001 to 2.011 / o can be added. The blood reserve pretreated in this way is about two to three times as high long shelf life as before. Of course, the chlorophyllin compound or the solution made from the alkali salts of chlorophyllin is sterile filtered and be free of suspended matter. For example, you can go to a set of 100 ccm of fresh blood, which in a known manner by the addition of 20 ccm of a 3.8% sodium citrate solution is made incoagulable, an alkali salt of chlorophyllin add in isotonic saline solution at a concentration of 2%. The used Alkali salts of chlorophyllin, such as chlorophyllin sodium, are a practically atoxic natural product, which also has no carcinogenic effect like some other dyes.
Claims (2)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DEP0018464 | 1957-05-03 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE1068862B true DE1068862B (en) | 1959-11-12 |
Family
ID=7367026
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DENDAT1068862D Pending DE1068862B (en) | 1957-05-03 | Procedure for extending the shelf life of blood products |
Country Status (3)
| Country | Link |
|---|---|
| DE (1) | DE1068862B (en) |
| FR (1) | FR1272491A (en) |
| GB (1) | GB901675A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE1164022B (en) * | 1960-05-31 | 1964-02-27 | Univ Minnesota | Method of preserving semen |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4082509A (en) * | 1976-08-05 | 1978-04-04 | Dow Corning Corporation | Method of storing blood and a blood storage bag therefor |
| ES2197168T3 (en) * | 1993-07-05 | 2004-01-01 | Sheffield Teaching Hospitals National Health Service Trust | PREPARATION AND STABILIZATION OF CELLS. |
| CN1149338A (en) * | 1994-04-05 | 1997-05-07 | 北方总医院N·H·S·托拉斯 | Preparation and stabilization of cell suspensions |
-
0
- DE DENDAT1068862D patent/DE1068862B/en active Pending
-
1959
- 1959-08-19 FR FR803256A patent/FR1272491A/en not_active Expired
- 1959-08-21 GB GB28576/59A patent/GB901675A/en not_active Expired
Non-Patent Citations (1)
| Title |
|---|
| None * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE1164022B (en) * | 1960-05-31 | 1964-02-27 | Univ Minnesota | Method of preserving semen |
Also Published As
| Publication number | Publication date |
|---|---|
| FR1272491A (en) | 1961-09-29 |
| GB901675A (en) | 1962-07-25 |
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