DD279165A1 - MEANS FOR INFLUENCING SPARKLING AND ROOTING FOR SPRING AND LAERCHE - Google Patents

Abstract

In the following, an agent is described, which consists of a combination of amino acids and phenolcarboxylic acids and in the in vitro cultivation of spruce and larch positively influences the shoot extension and rooting. Not only a quantitative improvement of rooting, but also a qualitative (fine root share) is achieved.

Description

Ausfühi ungsbeisptele

Four weeks old aseptically germinated spruce seedlings were used as hypocotyl cuttings (unfolded cotyledons + 15mm hypocoty!) 10mm deep with the hypocotyl in the solidified nutrient medium described below. To a BORNMAN base medium (Physiol Plant 57 (1983) 5-16), which was used at half concentration, was added 20 g of sucrose and 4.5 g of agar agar for solidification. In addition, an addition of 100 mg / l glutamine (GIn) and arginine (Arg) as well as different amounts of vanillin are carried out after autoclaving the nutrient media. The measurement of the shoot axis extension gave the following values:

Example 1:

variant

Sprooth Axial Extension (mm) Increase in%

Control (+ Arg, + GIn) 0.75

Combined with Arg, GIn + 50 mg / l vanillin 1.52

Combined with Arg, GIn + 25 mg / l vanillin 1.70

Combined with Arg, GIn + 1 mg / l vanillin 1.45

Combination with Arg, GIn + 1 mg / l p-coumaric acid 2.02

Combined with Arg, GIn + 25 mg / l p-coumaric acid 3.26

Combined with Arg, GIn + 50 mg / l p-coumaric acid 3.32

102.6 126.8 93.3 169.3 334.6 342.6

Example 2:

Hypocotyl cuttings of spruce were prepared according to Example 1 on a nutrient medium according to BORNMAN with 100 mg / l arginine and glutamine and various concentrations of vanillin. After 22 weeks of incubation, the rooting was scored.

variant

control

Combination with Arg + GIn + 1 mg / l vanillin combination with Arg + GIn + 10 mg / l vanillin combination with Arg + GIn + 50 mg / l vanillin

Example 3:

Hypocotyl cuttings of four week old aseptically grown spruce seedlings were incubated for adventitious bud induction in a solution containing 1 (10 mg / l kinetin (pH = 5.5) for 24 hours.

This was followed by a transfer to the nutrient medium described in Examples 1 and 2 with the addition of vanillin or p-coumaric acid.

20 weeks after the start of the test, the rooting was rated:

% -Bewurzelung % Plants with fine root 82.35 21.42 58.82 0 100.00 30.76 85,70 50,00

variant

% -Bewurzelung

% Plants with fine roots

Control 14.81

Combined with Arg + GIn + 50 mg / l vanillin 37.03

Combination with Arg + GIn + 25 mg / l p-coumaric acid 13,63

20.00 33.00

Example 4:

Established shoot tips of a hybrid larch clone (Larix eurolepis) were cultivated on a nutrient medium modified with reference to BOULAY (Ann. Rech. Sylvicoles, Afocel, Nangis, France 1976, 84-145) with 10 ^-3 M glutamine with the addition of the described substances was determined after 21 days:

control

Combination of 10 " ³ MGIn + 0.1 ppm p-coumaric acid combination of 10" ³ mgIn + 1.0 ppm p-coumaric acid combination of 10 " ³ mgIn + 10ppm p-coumaric acid combination of 10" ³ mgIn + 1 ppm vanillin

Sprout length increase (mm)

Claims (1)

Agent for influencing the shoot extension and rooting of spruce and larch with known combinations of phenolcarboxylic acids and amino acids, characterized in that as phenolcarboxylic vanillin and paracumaric acid in concentrations of 0.01 mg / l to 100mg / l with amino acids such as arginine and glutamine in concentrations of 0.1 mg / l to 200 mg / l. Field of application of the invention The invention relates to the field of propagation and growth control of spruce and larch. Characteristic of the known state of the art The generative and vegetative propagation of spruce and larch is often limited by insufficient seed germination, shoot elongation, poor rooting, especially fine roots and, in the case of rooting sprouts, irregular and absent root inductions. The stimulation of shoot elongation of intact plants with phytohormones, preferably gibberellins, is known and confirmed (WAGENBRETH et al., Progress Reports fd Agriculture and Food 19 [198115, page 14, DUNBERG and ODEN, Biochemistry and Physiology of Gibberellins (A.CROZIER, ed .), Praeger, New York 1983), but also cinnamic acid and benzoic acid derivatives (MICHNIEWICZ and GALOCH, Acta Soc., Bot., Pol., 43 [1974J, 273-281]) and the rooting of coniferous plug-in veins with auxins, preferably with indolyl-3 butyric acid (SU 719561). In addition to known auxins, other stimulators for the induction of adventitious roots such as aliphatic monocarboxylic acids (SU 990661), adenine derivatives (DE 2431392), aromatic compounds such as derivatives of cinnamic acid (GASPAR, T., Joint DPGRG and BPGRG Symp. Monogr. 6, 39-49) or flavonoid derivatives (US Pat. No. 4,152,869, ZIMMERMANN and FORDHAM, J. Amer Soc. Hort, Sci. 110 [1985] 34-38) for rooting shoots of woody plants. According to GASPAR (so 1980), for example, pine cuttings rooted under the influence of gibberellin A ₃ and the cinnamic acid derivatives chlorogenic and ferulic only when the substances are given after the induction phase, drug applications at the beginning of the experiment in the first four days suppress the training of roots. Root formations on sprouts, especially of newcomers to in vitro culture require their extension, which in spruce with known stimulators such as gibberollins, aliphatic monocarboxylic acids or auxins (BORNMAN 1987) insufficient or not, especially not when the by Kinetin-induced buds (according to VARNELL and VASIL, Amer., J. Bot., 65 [1978J, 47-49]) have been rendered dormant by this hormone. In addition to insufficient rooting / spontaneous rooting of sprouts, especially in conifers, there is a significant shortcoming in the qualitative root education in particular the expression of lateral and fine roots; These are not only a prerequisite for improved nutrient uptake, but also synthetic sites for the phytohormone group of cytokinins. Object of the invention The object of the invention is to promote physiological processes by finding agents which contribute to the influence of shoot elongation and root development, preferably to influence the fine root fraction in conifers. Explanation of the essence of the invention Accordingly, the object of the invention is to stimulate by means of per se known phenolic carboxylic acids in combination with amino acids in synthetic nutrient media, the Sproßs'.reckung the spruce and larch, d. h., to stretch stretched buds of these tree species, which are in a physiological rest and to improve the rooting qualitatively. Morkmale dar invention Agent for influencing germination and plant development by combination of known per se phenolcarboxylic acids and amino acids, characterized in that phenolic acids in concentrations of 0.01 mg / l to 100mg / l with amino acids, preferably arginine and glutamine in concentrations of 0.1 mg / l up to 200mg / l are used.