[go: up one dir, main page]

CZ81794A3 - Process for preparing gamma linoleic acid by the action of delta-6 desaturation - Google Patents

Process for preparing gamma linoleic acid by the action of delta-6 desaturation Download PDF

Info

Publication number
CZ81794A3
CZ81794A3 CZ94817A CZ81794A CZ81794A3 CZ 81794 A3 CZ81794 A3 CZ 81794A3 CZ 94817 A CZ94817 A CZ 94817A CZ 81794 A CZ81794 A CZ 81794A CZ 81794 A3 CZ81794 A3 CZ 81794A3
Authority
CZ
Czechia
Prior art keywords
nucleic acid
desaturase
isolated nucleic
gla
organism
Prior art date
Application number
CZ94817A
Other languages
English (en)
Other versions
CZ285471B6 (cs
Inventor
L Thomas Terry
S Reddy Avutu
Michael Nuccio
L Freyssinet Georges
Original Assignee
Rhone Poulenc Agrochimie
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Rhone Poulenc Agrochimie filed Critical Rhone Poulenc Agrochimie
Publication of CZ81794A3 publication Critical patent/CZ81794A3/cs
Publication of CZ285471B6 publication Critical patent/CZ285471B6/cs

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0083—Miscellaneous (1.14.99)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8247—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6472—Glycerides containing polyunsaturated fatty acid [PUFA] residues, i.e. having two or more double bonds in their backbone
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y114/00—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
    • C12Y114/19—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14) with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water (1.14.19)
    • C12Y114/19003—Linoleoyl-CoA desaturase (1.14.19.3)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
    • Y10S530/82—Proteins from microorganisms
    • Y10S530/825—Bacteria

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Nutrition Science (AREA)
  • Cell Biology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Fats And Perfumes (AREA)

Claims (19)

1. Izolovaná nukleová kyselina kódující bakteriální Á6-desaturázu.
2. Nukleová kyselina podle nároku 1 obsahující nukleotidy ze SEQ. ID N0:3.
3. Izolovaná nukleová kyselina, která kóduje pro sekvenci aminokyseliny kódovanou nukleovou kyselinou podle nároku 1.
4. Izolovaná nukleová kyselina podle kteréhokoli z nároků
1 až 3, kde zmíněná nukleová kyselina Je obsažena ve vektoru.
5. Izolovaná nukleová kyselina podle nároku 4 operačně napojená na promotor a/nebo terminační signál schopný účinného vyjádření genového produktu zmíněné izolované nukleové kyseliny.
6. Izolovaná nukleová kyselina podle nároku 5, kde zmíněný promotor je promotor Αβ-desaturázy, promotor Anabaena karboxylázy, promotor helianthininu, promotor glycinu, promotor napinu nebo specifický tkáňový promotor helianthininu.
Τ'. Izolovaná nukleová kyselina podle nároku 5, kde terminační signál je Synechocystle terminační signál, terminační signál syntézy nopalinu nebo terminační signál semena.
8. Izolovaná nukleová kyselina podle7 kteréhokoli z nároků 1 až 7, kde izolovaná nukleová kyselina je obsažena ve transgenickém organismu.
9. Izolovaná nukleová kyselina podle nároku 8, kde transgenický organismus je bakterie, houba, rostlinná buňka nebo zvíře.
10. Způsob produkce rostliny se zvýšeným obsahem gamma linolenové kyseliny (GLA), vyznačující se tím, že obsahuje kroky:
(a) transformaci rostlinné buňky izolovanou nukleovou kyselinou podle kteréhokoli z nároků 1 až 7 a (b) regeneraci rostliny se zvýšeným obsahem GLA ze zmíněné rostlinné buňky.
11. Způsob podle nároku 10, vyznačující se tím, že rostlina je slunečnice, sojový bob, kukuřice, tabák, arašid nebo řepka olejka.
12. Způsob indukce tvorby gamma linolenové kyseliny (GLA) v organismu se sníženým obsahem nebo s chybějící GLA, vyznačující se tím, že se zmíněný organismus transformuje izolovanou nukleovou kyselinou podle kteréhokoli z nároků 1 až 7.
-2013. Způsob indukce tvorby gamma linolenové kyseliny (GLA) v organismu se sníženým obsahem nebo s chybějící GLA a linolovou kyselinou (LA), vyznačující se tím, že se zmíněný organismus transformuje izolovanou nukleovou kyselinou kódující bakteriální A6-desaturázu a izolovanou nukleovou kyselinou kódující Al2-desaturázu.
14. Způsob indukce tvorby gamroa linolenové kyseliny (GLA) v organismu se sníženým obsahem nebo s chybějící GLA a linolovou kyselinou (LA)* vyznačující se tím, že se zmíněný organismus transformuje alespoň jedním vektorem vyjádření obsahujícím izolovanou nukleovou kyselinu kódující bakteriální A6-desaturázu a izolovanou nukleovou kyselinu kódující Al2-desaturézu.
15. Způsob podle kteréhokoli z nároků 13 nebo 14, vyznačující se tím, že zmíněná izolovaná nukleová kyselina kódující 66-desaturázu obsahuje nukleotidy 317 až 1507 ze SEQ. ID N0:l.
16. Způsob indukce tvorby oktadekatetraeonové kyseliny v organismu se sníženým obsahem nebo chybějící gamma linolenovou kyselinou, vyznačující se tím, že se zmíněný organismus transformuje izolovanou nukleovou kyselinou podle kteréhokoli z nároků 1 až 7.
17. Způsob podle nároku 16, vyznačující se tím, že organismus je bakterie, houba, rostlina nebo zvíře.
18. Způsob produkce rostlinjr se zvýšenou odolností proti chladu, vyznačující se tím, že obsahuje:
(a) transformaci rostlinné buňky izolovanou nukleovou kyselinou podle kteréhokoli z nároků 1 až 7 a (b) regeneraci zmíněné rostliny se zlepšenou odolností proti chladu ze zmíněné rostlinné buňky.
19. Způsob podle nároku 18, vyznačující se tím, že rostlina je slunečnice, sojový bob, kukuřice, tabák, arašid nebo řepka olejka.
20. Izolovaná bakteriální A6-desaturéza.
21. Izolovaná bakteriální A6-desaturáza podle nároku 20, vyznačujíc í ac- léByr=£e má sekvenci aminokyselin ze SEQ. ID NO:2.
CZ94817A 1991-10-10 1992-10-13 Způsob výroby gama linolenové kyseliny účinkem delta-6 Desaturázy CZ285471B6 (cs)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US77447591A 1991-10-10 1991-10-10
US81791992A 1992-01-08 1992-01-08
PCT/US1992/008746 WO1993006712A1 (en) 1991-10-10 1992-10-13 Production of gamma linolenic acid by a δ6-desaturase

Publications (2)

Publication Number Publication Date
CZ81794A3 true CZ81794A3 (en) 1995-09-13
CZ285471B6 CZ285471B6 (cs) 1999-08-11

Family

ID=27118889

Family Applications (1)

Application Number Title Priority Date Filing Date
CZ94817A CZ285471B6 (cs) 1991-10-10 1992-10-13 Způsob výroby gama linolenové kyseliny účinkem delta-6 Desaturázy

Country Status (23)

Country Link
US (3) US5552306A (cs)
EP (1) EP0666918B1 (cs)
JP (1) JP3537434B2 (cs)
KR (1) KR100316068B1 (cs)
CN (2) CN1053469C (cs)
AT (1) ATE284961T1 (cs)
AU (1) AU667848B2 (cs)
BG (1) BG61791B1 (cs)
BR (1) BR9206613A (cs)
CA (1) CA2120629C (cs)
CZ (1) CZ285471B6 (cs)
DE (1) DE69233459T2 (cs)
DK (1) DK0666918T3 (cs)
ES (1) ES2231767T3 (cs)
HU (1) HU217328B (cs)
IL (1) IL103407A (cs)
MX (1) MX9205820A (cs)
NZ (1) NZ244685A (cs)
PH (1) PH31293A (cs)
RO (1) RO113256B1 (cs)
RU (1) RU2152996C2 (cs)
UA (1) UA43314C2 (cs)
WO (1) WO1993006712A1 (cs)

Families Citing this family (122)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5614393A (en) * 1991-10-10 1997-03-25 Rhone-Poulenc Agrochimie Production of γ-linolenic acid by a Δ6-desaturase
US20070130654A1 (en) * 1991-10-10 2007-06-07 Thomas Terry L Production of gamma linolenic acid by a delta6-desaturase
US6872872B1 (en) 1992-11-17 2005-03-29 E. I. Du Pont De Nemours And Company Genes for microsomal delta-12 fatty acid desaturases and related enzymes from plants
US6372965B1 (en) 1992-11-17 2002-04-16 E.I. Du Pont De Nemours And Company Genes for microsomal delta-12 fatty acid desaturases and hydroxylases from plants
EP0684998A1 (en) 1993-02-05 1995-12-06 Monsanto Company Altered linolenic and linoleic acid content in plants
US5639645A (en) * 1993-09-22 1997-06-17 Mitsubishi Corporation Recombinant Δ9 desaturase and a gene encoding the same
US6310194B1 (en) * 1994-09-26 2001-10-30 Carnegie Institution Of Washington Plant fatty acid hydroxylases
US5965793A (en) 1995-09-20 1999-10-12 Monsanto Company, Inc. Strong early seed-specific gene regulatory region
US5959175A (en) * 1997-04-09 1999-09-28 Thomas; Terry L. Sunflower albumin 5' regulatory region for the modification of plant seed lipid composition
US5977436A (en) 1997-04-09 1999-11-02 Rhone Poulenc Agrochimie Oleosin 5' regulatory region for the modification of plant seed lipid composition
US6428990B1 (en) 1997-04-11 2002-08-06 Abbott Laboratories Human desaturase gene and uses thereof
US6075183A (en) * 1997-04-11 2000-06-13 Abbott Laboratories Methods and compositions for synthesis of long chain poly-unsaturated fatty acids in plants
US6051754A (en) * 1997-04-11 2000-04-18 Abbott Laboratories Methods and compositions for synthesis of long chain poly-unsaturated fatty acids in plants
US5972664A (en) * 1997-04-11 1999-10-26 Abbott Laboratories Methods and compositions for synthesis of long chain poly-unsaturated fatty acids
US7745694B1 (en) 1997-04-11 2010-06-29 Monsanto Technology Llc Methods and compositions for synthesis of long chain polyunsaturated fatty acids in plants
US5968809A (en) * 1997-04-11 1999-10-19 Abbot Laboratories Methods and compositions for synthesis of long chain poly-unsaturated fatty acids
SK139999A3 (en) * 1997-04-11 2000-05-16 Calgene Llc A nucleic acids construct, isolated nucleotide sequence, recombinant plant cell, method of biosynthesis of polyunsaturated long chain fatty acids, pharmaceutical, food and cosmetic preparation and use thereof
US6432684B1 (en) 1997-04-11 2002-08-13 Abbott Laboratories Human desaturase gene and uses thereof
IL132393A0 (en) * 1997-04-15 2001-03-19 Commw Scient Ind Res Org Plant fatty acid epoxygenase genes and uses therefor
US6080911A (en) * 1997-04-15 2000-06-27 Ohio University Mice models of growth hormone insensitivity
US6100450A (en) * 1997-10-22 2000-08-08 Rhone-Poulenc Agrochimie Seed specific promoters based on arabidopsis genes
GB9724783D0 (en) * 1997-11-24 1998-01-21 Inst Arable Crops Research Novel polypeptides
AU4544799A (en) * 1998-05-29 1999-12-13 Ohio University Compositions and methods for the synthesis of fatty acids, their derivatives and downstream products
EP1895009A1 (en) * 1998-06-12 2008-03-05 Calgene LLC Polyunsaturated fatty acids in plants
US6913916B1 (en) 1998-09-02 2005-07-05 Abbott Laboratories Elongase genes and uses thereof
US6677145B2 (en) 1998-09-02 2004-01-13 Abbott Laboratories Elongase genes and uses thereof
US20030163845A1 (en) * 1998-09-02 2003-08-28 Pradip Mukerji Elongase genes and uses thereof
US6403349B1 (en) 1998-09-02 2002-06-11 Abbott Laboratories Elongase gene and uses thereof
PL357415A1 (en) 1999-06-07 2004-07-26 Basf Plant Science Gmbh Delta6-acetylenase and delta6-desaturase from ceratodon purpureus
RU2268939C2 (ru) * 1999-06-07 2006-01-27 Басф Плант Сайенс Гмбх ДЕЛЬТА 6-АЦЕТИЛЕНАЗА/ДЕЛЬТА-6-ДЕСАТУРАЗА И ДЕЛЬТА-6-ДЕСАТУРАЗА ИЗ Ceratodon purpureus
DE10030976A1 (de) 2000-06-30 2002-01-10 Basf Ag DELTA6-Desaturasegene exprimierende Pflanzen und PUFAS enthaltende Öle aus diesen Pflanzen und ein Verfahren zur Herstellung ungesättigter Fettsäuren
US7070970B2 (en) 1999-08-23 2006-07-04 Abbott Laboratories Elongase genes and uses thereof
GB9929897D0 (en) * 1999-12-18 2000-02-09 Slabas Antoni R Improvements in or relating to conjugated fatty acids and related compounds
FR2803592A1 (fr) 2000-01-06 2001-07-13 Aventis Cropscience Sa Nouveaux derives de l'acide 3-hydroxypicolinique, leur procede de preparation et compositions fongicides les contenant.
RU2311457C2 (ru) * 2000-02-09 2007-11-27 Басф Акциенгезельшафт Новый ген элонгазы и способ получения полиненасыщенных кислот жирного ряда
FR2810994B1 (fr) * 2000-06-30 2004-03-12 Biogemma Fr Acides nucleiques codant pour une phosphatase vegetale de type mipp a activite phytasique et applications
EP1197550A3 (en) * 2000-08-25 2002-11-20 Pfizer Products Inc. Methods and compositions for diagnosing and treating disorders involving angiogenesis
CN105483142A (zh) * 2000-09-28 2016-04-13 生物源食物及科学公司 脂肪酸去饱和酶家族成员fad4、fad5、fad5-2和fad6及它们的应用
FR2815969B1 (fr) 2000-10-30 2004-12-10 Aventis Cropscience Sa Plantes tolerantes aux herbicides par contournement de voie metabolique
DE10102337A1 (de) 2001-01-19 2002-07-25 Basf Plant Science Gmbh Verfahren zur Herstellung mehrfach ungesättigter Fettsäuren, neue Biosynthesegene sowie neue pflanzliche Expressionskonstrukte
US6635451B2 (en) 2001-01-25 2003-10-21 Abbott Laboratories Desaturase genes and uses thereof
BR0205508A (pt) * 2001-01-25 2005-05-03 Abbott Lab Genes para dessaturase e usos dos mesmos
US7045683B2 (en) * 2001-05-04 2006-05-16 Abbott Laboratories Δ4-desaturase genes and uses thereof
WO2002092540A1 (en) * 2001-05-14 2002-11-21 Martek Biosciences Corporation Production and use of a polar lipid-rich fraction containing omega-3 and/or omega-6 highly unsatruated fatty acids from microbes, genetically modified plant seeds and marine organisms
US20070004678A1 (en) * 2001-05-14 2007-01-04 Gerhard Kohn Production and use of a polar lipid-rich fraction containing stearidonic acid and gamma linolenic acid from plant seeds and microbes
ES2337564T3 (es) 2002-01-30 2010-04-27 Basf Plant Science Gmbh Metodo para elaborar acidos grasos poliinsaturados mediante un nuevo gen de elongasa.
US7211656B2 (en) * 2002-01-30 2007-05-01 Abbott Laboratories Desaturase genes, enzymes encoded thereby, and uses thereof
GB2385852A (en) 2002-02-27 2003-09-03 Rothamsted Ex Station Delta 6-desaturases from Primulaceae
US20040025202A1 (en) * 2002-03-15 2004-02-05 Laurie Cathy C. Nucleic acid molecules associated with oil in plants
DE10219203A1 (de) 2002-04-29 2003-11-13 Basf Plant Science Gmbh Verfahren zur Herstellung mehrfach ungesättigter Fettsäuren in Pflanzen
EP2216405A1 (en) 2002-05-03 2010-08-11 Monsanto Technology LLC Speed specific USP promoters for expressing genes in plants
FR2848064B1 (fr) * 2002-12-06 2006-01-13 Bayer Cropscience Sa Plantes legumineuses transplastomiques fertiles
FR2848570B1 (fr) 2002-12-12 2005-04-01 Bayer Cropscience Sa Cassette d'expression codant pour une 5-enol pyruvylshikimate-3-phosphate synthase (epsps) et plantes tolerantes aux herbicides la contenant
US7078234B2 (en) 2002-12-18 2006-07-18 Monsanto Technology Llc Maize embryo-specific promoter compositions and methods for use thereof
EP1576166B1 (en) 2002-12-19 2014-10-15 University Of Bristol Novel method for the production of polyunsaturated fatty acids
AU2004215705B2 (en) 2003-02-27 2009-11-12 Basf Plant Science Gmbh Method for the production of polyunsaturated fatty acids
WO2004087878A2 (en) 2003-03-28 2004-10-14 Monsanto Technology, Llc Novel plant promoters for use in early seed development
EP2390313B1 (de) 2003-03-31 2016-05-11 University Of Bristol Neue pflanzliche Acyltransferase spezifisch für langkettige mehrfach ungesättigte Fettsäuren
MXPA05010571A (es) 2003-04-08 2005-11-23 Basf Plant Science Gmbh Delta-4-desaturasas a partir de euglena gracilis, que expresan plantas y aceites que comprenden pufa
US11952581B2 (en) 2003-08-01 2024-04-09 Basf Plant Science Gmbh Process for the production of polyunsaturated fatty acids in transgenic organisms
MX347962B (es) 2003-08-01 2017-05-19 Basf Plant Science Gmbh * Metodo para la produccion de acidos grasos poli-insaturados en organismos transgenicos.
AR045290A1 (es) 2003-08-21 2005-10-19 Monsanto Technology Llc Acido graso desaturasas de primula
CA2450000A1 (en) * 2003-12-18 2005-06-18 Alberta Research Council Inc. Method of creating plants with reduced level of saturated fatty acid in seed oil
MXPA06009572A (es) 2004-02-27 2007-05-23 Basf Plant Science Gmbh Metodo para la produccion de acidos grasos poliinsaturados en plantas transgenicas.
PL1720988T3 (pl) 2004-02-27 2012-05-31 Basf Plant Science Gmbh Sposób wytwarzania nienasyconych kwasów tłuszczowych omega-3 w organizmach transgenicznych
US20050220901A1 (en) * 2004-03-22 2005-10-06 Huttenbauer Samuel Jr Methods of pharmaceutical separation from plants
EP2902021B1 (en) 2004-04-16 2018-11-14 Monsanto Technology LLC Expression of fatty acid desaturases in corn
CA3023314C (en) 2004-04-22 2019-12-10 Commonwealth Scientific And Industrial Research Organisation Synthesis of long-chain polyunsaturated fatty acids by recombinant cells
US7807849B2 (en) 2004-04-22 2010-10-05 Commonwealth Scientific And Industrial Research Organisation Synthesis of long-chain polyunsaturated fatty acids by recombinant cells
US7138391B2 (en) * 2004-08-09 2006-11-21 Silverbrook Research Pty Ltd Hydrophilizable and hydrophilic cyanine dyes
US7456270B2 (en) * 2004-09-01 2008-11-25 Abbott Laboratories Δ6-desaturase genes and uses thereof
CN101128591B (zh) 2005-02-26 2013-02-13 巴斯福植物科学有限公司 用于植物中种子偏好性表达的表达盒
DE102005013779A1 (de) 2005-03-22 2006-09-28 Basf Plant Science Gmbh Verfahren zur Herstellung von mehrfach ungesättigten C20- und C22-Fettsäuren mit mindestens vier Doppelbindungen in transgenen Pflanzen
ATE552343T1 (de) 2005-04-19 2012-04-15 Basf Plant Science Gmbh Endosperm-spezifische expression und/oder expression in keimenden embryos monokotyledoner pflanzen
CA2605393A1 (en) 2005-04-20 2006-10-26 Basf Plant Science Gmbh Expression cassettes for seed-preferential expression in plants
EP1882037A2 (en) 2005-05-10 2008-01-30 BASF Plant Science GmbH Expression cassettes for seed-preferential expression in plants
JP5033122B2 (ja) 2005-05-23 2012-09-26 アルカディア バイオサイエンシズ，インコーポレイテッド 増量ガンマ−リノレン酸を含むベニバナ
DE102005038036A1 (de) 2005-08-09 2007-02-15 Basf Plant Science Gmbh Verfahren zur Herstellung von Arachidonsäure und/oder Eicosapentaensäure in transgenen Nutzpflanzen
GB2431158A (en) 2005-10-13 2007-04-18 Rothamsted Res Ltd Process for the production of arachidonic and/or eicosapentaenoic acid
DE102005052551A1 (de) 2005-11-02 2007-05-16 Rothamsted Res Harpenden Verfahren zur Herstellung von y-Linolensäure und/oder Stearidonsäure in transgenen Brassicaceae und Linaceae
EP1806398A1 (en) * 2006-01-04 2007-07-11 Monsanto S.A.S. Fad-2 mutants and high oleic plants
GB0603160D0 (en) 2006-02-16 2006-03-29 Rothamsted Res Ltd Nucleic acid
AR059376A1 (es) 2006-02-21 2008-03-26 Basf Plant Science Gmbh Procedimiento para la produccion de acidos grasos poliinsaturados
US8629195B2 (en) 2006-04-08 2014-01-14 Bayer Materialscience Ag Production of polyurethane foams
US20080043087A1 (en) 2006-07-19 2008-02-21 James Cowan Digital transfer method for printing on a target surface
WO2008022963A2 (en) 2006-08-24 2008-02-28 Basf Plant Science Gmbh Isolation and characterization of a novel pythium omega 3 desaturase with specificity to all omega 6 fatty acids longer than 18 carbon chains
WO2008025068A1 (en) 2006-08-29 2008-03-06 Commonwealth Scientific And Industrial Research Organisation Synthesis of fatty acids
EP3066934A1 (de) 2006-10-06 2016-09-14 BASF Plant Science GmbH Verfahren zur herstellung mehrfach ungesättigter fettsäuren in transgenen organismen
CN101631868B (zh) 2007-02-16 2016-02-10 巴斯福植物科学有限公司 用于在单子叶植物中调节胚特异性表达的核酸序列
SG182157A1 (en) 2007-06-01 2012-07-30 Solazyme Inc Production of oil in microorganisms
EP2176416B1 (de) 2007-07-31 2016-03-16 BASF Plant Science GmbH Elongasen und verfahren zur herstellung mehrfach ungesättigter fettsäuren in transgenen organismen
US20130323801A1 (en) * 2007-11-01 2013-12-05 Wake Forest University School Of Medicine Compositions, Methods, and Kits for Polyunsaturated Fatty Acids from Microalgae
DE112008003237T5 (de) 2007-12-14 2011-03-17 Basf Plant Science Gmbh Promotoren von Brassica napus für samenspezifische Genexpression
US8785727B2 (en) 2008-04-30 2014-07-22 Rothamsted Research Ltd. Desaturase and method for the production of polyunsaturated fatty acids in transgenic organisms
CA2989798C (en) 2008-07-01 2019-11-05 Basf Plant Science Gmbh Promoters from brassica napus for seed specific gene expression
JP5757870B2 (ja) 2008-08-26 2015-08-05 ビーエーエスエフ プラント サイエンス ゲーエムベーハー デサチュラーゼをコードする核酸および改変植物油
CN102317459A (zh) 2008-11-18 2012-01-11 联邦科学技术研究组织 产生ω-3脂肪酸的酶和方法
AU2009319721B2 (en) 2008-11-28 2016-01-14 Corbion Biotech, Inc. Manufacturing of tailored oils in recombinant heterotrophic microorganisms
PL2376638T3 (pl) 2008-12-12 2014-01-31 Basf Plant Science Gmbh Desaturazy oraz sposób wytwarzania wielonienasyconych kwasów tłuszczowych w organizmach transgenicznych
AR074941A1 (es) 2009-01-07 2011-02-23 Bayer Cropscience Sa Plantas transplastomicas exentas de marcador de seleccion
CA2758824A1 (en) 2009-04-17 2010-10-21 Basf Plant Science Company Gmbh Plant promoter operable in endosperm and uses thereof
UA109110C2 (ru) 2009-04-22 2015-07-27 Басф Плант Саєнс Компані Гмбх Полинуклеотид, который обеспечивает возможность специфической экспрессии в целых семенах однодольного растения
WO2010130725A1 (en) 2009-05-13 2010-11-18 Basf Plant Science Company Gmbh Acyltransferases and uses thereof in fatty acid production
BR112012000448A2 (pt) 2009-07-10 2015-10-06 Basf Plant Science Gmbh cassete de expressão para a regulação da expressão semente-específica de um polinucleotídeo de interesse, vetor, célula hospedeira, tecido de planta, órgão de planta, planta ou semente transgênicos, metodo para expressar um polinucleotídeo de interesse em uma célula hospedeira, métodos para produzir um tecido vegetal, órgão de planta, planta, ou semente transgênicos e uso do cassete de expressão
US8188335B2 (en) 2009-07-17 2012-05-29 Abbott Laboratories Δ9-elongase for production of polyunsaturated fatty acid-enriched oils
CA2782290A1 (en) 2009-12-03 2011-06-09 Basf Plant Science Company Gmbh Expression cassettes for embryo-specific expression in plants
CA2788052C (en) 2010-02-02 2020-02-04 Bayer Cropscience Ag Soybean transformation using hppd inhibitors as selection agents
AU2011257982B2 (en) 2010-05-28 2017-05-25 Corbion Biotech, Inc. Tailored oils produced from recombinant heterotrophic microorganisms
WO2011161093A1 (en) 2010-06-25 2011-12-29 Basf Plant Science Company Gmbh Acyltransferases and uses therof in fatty acid production
CN103282473A (zh) 2010-11-03 2013-09-04 索拉兹米公司 具有降低倾点的微生物油、从其中产生的介电流体、以及相关方法
CN103827307A (zh) 2011-02-02 2014-05-28 索拉兹米公司 产自重组产油微生物的定制油
WO2013153404A1 (en) 2012-04-12 2013-10-17 Rothamsted Research Ltd Production of omega-3 long chain polyunsaturated fatty acids
US8945908B2 (en) 2012-04-18 2015-02-03 Solazyme, Inc. Tailored oils
US9719114B2 (en) 2012-04-18 2017-08-01 Terravia Holdings, Inc. Tailored oils
HUE033766T2 (en) 2012-06-15 2018-01-29 Commw Scient Ind Res Org Production of long chain polyunsaturated fatty acids in plant cells
GB201217524D0 (en) 2012-10-01 2012-11-14 Rothamsted Res Ltd Recombinant organisms
US9249252B2 (en) * 2013-04-26 2016-02-02 Solazyme, Inc. Low polyunsaturated fatty acid oils and uses thereof
BR112016006839A8 (pt) 2013-10-04 2017-10-03 Solazyme Inc Óleos customizados
CA3241340A1 (en) 2013-12-18 2015-06-25 Grains Research And Development Corporation Lipid comprising long chain polyunsaturated fatty acids
EP3160482A4 (en) 2014-06-27 2018-02-14 Commonwealth Scientific and Industrial Research Organisation Lipid comprising docosapentaenoic acid
CN106574255A (zh) 2014-07-10 2017-04-19 泰拉瑞亚控股公司 酮脂酰acp合酶基因及其用途
KR102054762B1 (ko) 2018-03-06 2020-01-22 대한민국(관리청: 특허청장, 승계청: 농촌진흥청장) 갈고리 흰오징어 유래의 지방산 사슬연장효소 유전자 및 이의 용도

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4736866A (en) * 1984-06-22 1988-04-12 President And Fellows Of Harvard College Transgenic non-human mammals
NZ221259A (en) * 1986-07-31 1990-05-28 Calgene Inc Seed specific transcriptional regulation
CA1333397C (en) * 1987-11-04 1994-12-06 Jean Bernard Ducep Fluorinated arachidonic acid derivatives
DK0413019T3 (da) * 1989-02-24 2001-11-12 Monsanto Technology Llc Syntetiske plantegener og fremgangsmåde til fremstilling af disse
DE69133261D1 (de) * 1990-03-16 2003-06-26 Calgene Llc Davis Dnas, die für pflanzliche desaturasen kodieren und deren anwendungen

Also Published As

Publication number Publication date
EP0666918B1 (en) 2004-12-15
JPH07503605A (ja) 1995-04-20
KR100316068B1 (ko) 2002-11-13
RU2152996C2 (ru) 2000-07-20
UA43314C2 (uk) 2001-12-17
BG98695A (bg) 1995-05-31
WO1993006712A1 (en) 1993-04-15
CZ285471B6 (cs) 1999-08-11
BR9206613A (pt) 1995-04-11
MX9205820A (es) 1993-04-01
EP0666918A1 (en) 1995-08-16
EP0666918A4 (en) 1995-03-17
BG61791B1 (bg) 1998-06-30
DE69233459D1 (de) 2005-01-20
HU9401007D0 (en) 1994-08-29
NZ244685A (en) 1994-06-27
CN1121499C (zh) 2003-09-17
IL103407A0 (en) 1993-03-15
HUT69781A (en) 1995-09-28
CN1174236A (zh) 1998-02-25
CA2120629C (en) 2009-02-17
ATE284961T1 (de) 2005-01-15
CN1053469C (zh) 2000-06-14
AU667848B2 (en) 1996-04-18
DK0666918T3 (da) 2005-03-14
DE69233459T2 (de) 2005-12-15
HU217328B (hu) 1999-12-28
US5689050A (en) 1997-11-18
JP3537434B2 (ja) 2004-06-14
PH31293A (en) 1998-07-06
US5552306A (en) 1996-09-03
RO113256B1 (ro) 1998-05-29
AU2881292A (en) 1993-05-03
ES2231767T3 (es) 2005-05-16
IL103407A (en) 1999-09-22
CA2120629A1 (en) 1993-04-15
CN1072722A (zh) 1993-06-02
US5663068A (en) 1997-09-02

Similar Documents

Publication Publication Date Title
CZ81794A3 (en) Process for preparing gamma linoleic acid by the action of delta-6 desaturation
RU2181772C2 (ru) Синтез гамма-линоленовой кислоты под действием дельта-6-десатуразы
US6355861B1 (en) Production of gamma linolenic acid by a Δ6-desaturase
EP1656449B1 (en) Fatty acid desaturases from primula
US6683232B1 (en) Production of γ linolenic acid by a Δ6-desaturase
US7615364B2 (en) Polypeptide having activity of unsaturating W3-fatty acid, polynucleotide coding for the polypeptide and use thereof
US20070130654A1 (en) Production of gamma linolenic acid by a delta6-desaturase
WO2011010485A1 (ja) エイコサノイド生産方法、並びにゼニゴケ由来のエイコサノイド生合成系遺伝子及びその利用
US20090077692A1 (en) Production of gamma linolenic acid by a delta6-desaturase

Legal Events

Date Code Title Description
IF00 In force as of 2000-06-30 in czech republic
MK4A Patent expired

Effective date: 20121013