CY1399A

CY1399A - 4,5,5a-6-tetrahydrodibenz(cd,f) indoles

Info

Publication number: CY1399A
Application number: CY139981A
Authority: CY
Original assignee: Sandoz Ltd
Priority date: 1980-06-27
Filing date: 1981-06-24
Publication date: 1987-12-18
Also published as: FI75153B; ATA284481A; FI811922L; FR2485530B1; PH22773A; ES8300701A1; CA1155447A; PT73258A; ES8400089A1; ES515568A0; GB2136418A; AT380875B; DE3124086A1; KE3749A; IE811421L; ES515567A0; IE51341B1; GB2136418B; SE8103983L; AU546599B2

Description

1

GB 2 078 225 A - 1

SPECIFICATION

Heterocyclic compounds, their preparation and pharmaceutical compositions containing them

The present invention relates to phenanthrene derivatives, their preparation and pharmaceutical compositions containing them.

5 Belgian patent n° 877 169 describes a class of 4,5,5a,6-tetrahydro-dibenz[cd,f]indole derivatives 5 having stimulant activity on central dopaminergic receptors. All the compounds specifically exemplified which have alkyl groups attached to positions 4 and 5, contain as one alkyl group a methyl group. It has been now surprisingly found that a group of (4R*,5aS*)-4,5,5a,6-tetrahydro-dibenz[cd,f]indoie derivatives having ethyl and n-propyl groups in the 4 and 5 positions, which are nowhere specifically , 10 described or suggested in this patent, possess a particularly interesting pharmacological profile, inter 10 alia, long duration of action and/or notable potency as central dopaminergic agents and good tolerability.

In accordance with the invention there are provided compounds of formula I,

15 wherein 1 g

R, and R2 independently, are ethyl or n-propyl radicals, and the R3 substituents are the same and are hydroxy or acyloxy radicals,

in racemicform having the relative configuration 4R*,5aS*, or in optically active isomer form having the absolute configuration 4S,5aR.

20 The acyloxy radicals are conveniently radicals of formula 20

R— CO—Clin which Ra is an alkyl radical, (C3_7)cycloalkyl, a phenyl radical or a 5- or 6-membered heterocyclic ring.

When Ra is an alkyl radical, this may conveniently contain 1—17 carbon atoms, preferably 1 to 7 carbon atoms, and may be straight chain or branched chain.

25 Ra may be a substituted alkyl radical, containing in the alkyl chain conveniently 1—5 carbon 25

atoms. Conveniently it is a monosubstituted alkyl radical. The substituents may be, e.g., carboxy,

hydroxy, amino, (C1_4)alkylamino, (C,_4)alkoxy, dMC^Jalkyiamino, halogen, (C^Jalkylthio, phenoxy, a phenyl radical, 1 -pyrrolidinyl, piperidino or morpholino, and conveniently a phenyl radical. R, may also be e.g. an alkyl radical containing in the alkyl chain 1—4 carbon atoms and substituted by (C3_7)cycloalkyl. 30 When Ra is a phenyl radical or alkyl substituted by a phenyl radical, the phenyl radical may bs 30 unsubstituted or contain 1,2 or 3 identical or different substituents, selected, for example, from halogen, trifluoromethyl, {C1_4)alkyl, {C^Jalkoxy, (C1_4)alkylthlo and di-fC^Jalkylamino, or a methylenedioxy group. Preferably the phenyl ring is mono- or disubstituted.

When Ra is an alkyl substituted by a phenyl radical, Ra is preferably a benzyl radical. The phenyl 35 ring may bear substituents selected, for example, from halogen, (C,_4)alkyl, (C^Jalkoxy or 35

(C1_4)alkyIthio.

When Ra is a heterocyclic ring, this is suitably a heterocycle containing oxygen, nitrogen or sulfur as sole heteroactom, e.g. pyridine, thiophene orfuran.

Preferably Ra is (C,_7]alkyl; (C3_6)cycloalkyl; unsubstituted phenyl; phenyl mono- or disubstituted * 40 by chlorine, fluorine, trifluoromethyl, (C1_4)alkyl or (C.,_4)alkoxy; unsubstituted benzyl; or benzyl mono- 40 or disubstituted by chlorine, fluorine, <C1_4)aIkyl or (C^alkoxy.

Halogen may signify chlorine, bromine or fluorine, preferably chlorine or fluorine.

? In a group of compounds, R, is n-propyl and R2 is ethyl. In another group of compounds, R, and R2

are the same and signify ethyl orn-propyl. Preferably in the compounds of formula I the R3 substituents 45 are hydroxy. The preferred compounds are the (4S,5aR) optical isomers. 45

More particularly the invention provides a process for the production of compounds of formula I,

which comprises > a) producing a compound of formula la

50 wherein R, and R2 are as defined above, by splitting the ether groups Z of a compound of formula II

BNSDOCID: <GB 2078225A_1_>

GB 2 078 225 A

wherein R, and R2 are defined above and Z is a splittable ether group, in racemic form having the relative configuration 4R*,5aS*, or in optically active isomer form having the absolute configuration 4S,5aR, or

5 b) producing a compound of formula lb 5

wherein R, and R2 are defined above and the radicals are identical acyloxy radicals, by acylating a compound of formula la in racemic form having the relative configuration 4R*,5aS*orin optically active isomer form having the absolute configuration 4S,5aR.

10 The ether splitting process according process a) may be effected in conventional manner for 10

splitting ether groups. For example the reaction may be carried out by treatment of the starting materials with a strong mineral acid, e.g. hydrobromic or hydroiodic acid, at a temperature of at least 100°C, preferably from 100°C to the boiling point of the reaction mixture, especially at about 130°C. The ether group Z is preferably a methoxy radical.

15 The acylation process according to process b), may be effected in conventional manner for the 15

selectivs acylation of phenolic groups in the presence of an amine function. For example there may be used, as acylating agent, a functional derivative of an acid such an acid chloride, acid bromide or the acid anhydride. Conveniently the reaction is carried out by reacting an acid chloride in the presence of trifluoroacetic acid at temperatures frofm 20°C to the boiling point of the reaction mixture or in the 20 presence of pyridine at temperature from 0°C to room temperature. 20

The resulting compounds of formula I may be isolated from the reaction mixture and purified in known manner. The free base forms of compounds of formula I may be converted into acid addition salt forms in conventional manner and vice versa. Suitable acids for salt formation are, for example,

hydrochloric acid, tartaric acid, di-0,0-p-toluoyl-D- or L-tartaric acid, and hydrobromic acid. 25 Racemic compounds of formula I may be obtained from racemic starting materials. Optically 25

active isomers of formula I may be obtained from optically active precursors with the configuration (4S,5aR) or from the racemate. The 4S,5aR enantiomer may be obtained from racemate by known methods, for example by fractional crystallization of diastereoisomeric salts, e.g. their salts with {+)-di-0,0-p-toiuoyl-D-tartaric acid or {—)-di-0,0-p-toluoyl-L-tartaric acid. Racemic resolution into the 30 optically active isomers may be effected preferably at an early stage in the synthesis, e.g. before 30

splitting of the ether groups.

The starting materials of formula II may be prepared by reducing compounds of formula 111

wherein Rv R2 and R3 are as defined above.

35 The reduction may be effected conveniently under acidic conditions suitable for the acidic 35

reduction of enamines or imines, for example with zinc in an aqueous mineral acid, preferably hydrochloric acid, conveniently in the presence of a mercury (II) salt, for example mercury{!l) chloride. The reaction may suitably be effected for example in ethanol, and at temperatures from 50°C to the boiling point of the reaction mixture.

40 Starting materials of formula 111 may be prepared, for example, according the following reaction 40

scheme:

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ter t.-butyl 4 lithium '

R -MgBr or Rj-Li

(IT)

N-CO-R,

0 (VI)

.Hal-CO-R,

(V)

(Til)

•tiutyllithiuni

(Till)

(IX)

NH-CO-RJ

R'-CO-Hal

NaN3

COOH

W

10

tn the reaction scheme the radicals R„ R2 and Z are as defined above and R2 is methyl or ethyl. The reactions may be carried out in conventional manner and the products of the above reactions may be isolated and purified in known manner.

In the above intermediates the ether groups Z are conveniently methoxy.

Insofar the preparation or any particular starting material is not particularly described, this may be effected in conventional manner. For example the phenanthrene derivatives which are starting materials of formula XI, are described in the Elsevier's Encyclopaedia of Organic Chemistry, vol. 13, Tricyclic compounds, Elsevier Publishing Company Inc. New York (1946).

In the following Examples all temperatures are given in degrees Celsius and are uncorrected.

10

EXAMPLE 1

a) 9-(N-ethyl-N-butyryl-amino)-3-bromo-3,4-dimethoxy-phenanthrene Icompound of formula IV)]

A solution of 50 g (138 mM) 9-ethylamino-8-bromo-3,4-dimethoxy-phenanthrene in 57.5 ml (333 mM) N-ethyl-diisopropylamine and 420 ml methylene chloride is added dropwise, with stirring, 15 under a nitrogen atmosphere over a period of 20 minutes to a solution of 28.8 ml (276 mM) butyryl 15 chloride in 420 ml methylene chloride. During the addition the reaction mixture is maintained at room

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GB 2 078 225 A • 4

temperature by cooling in a water bath. The resultant yellow-red solution is then worked up in known manner to afford 9-(N-ethyl-N-butyryI-amino)-8-bromo-3,4-dimethoxy-phenanthrene; IW.pt 102—104°.

b) (4RS)-5-ethyl-4,5-dihydo-4-hydroxy-9,10-dimethoxy-4-/7-propyl-dibenz[cd,f]indole [compound of 5 formula III]

25 g (58.14 mM) of 9-(N-ethyl-N-butyryIamino)-8-bromo-3,4-dimethoxy phenanthrene are dissolved with stirring in 450 ml anhydrous tetrahydrofuran and the solution obtained is cooled to —25° with a bath of methylene chloride and dry ice. 73 ml (116.28 mM) of a 1.7 molar solution of fe/t.-butyl-lithium in pentane are then added dropwise over 4 minutes. Then the temperature of the reaction 10 mixture is allowed to increase to +5° over a period of 30 minutes, the reaction mixture is poured onto 500 ml of a mixture water and ice, extracted three times with, each time, 500 mi methylene chloride, and the organic phases are washed with water, dried and evaporated. After the product is dried in high vacuum, there is obtained (4RS)-5-ethyl-4,5-dihydro-4-hydroxy-9,10-dimethoxy-4-n-propyl-dibenz[cd,f]indole in the form of a yellow green foam.

15 c) (±)-(4R*,5aS*)-5-ethyl-4,5,5a,6-tetrahydro-9,10-dimethoxy-4-n-propyl-dibenz[cd,f]indole [compound of formula II]

A suspension of 40.6 g (116 mM) of (4RS)-5-ethyl-4,5-dihydro-4-hydroxy-9,10-dimethoxy-4-/j-propyl-dibenz[cd,f]indole in 1060 ml ethanol is added with stirring to a suspension of 140 g of zinc dust and 31.6 g (116 mM) of mercury (II) chloride in 1060 ml distilled water (alternatively the zinc . 20 dust/mercury chloride may be added to the dibenzindole). The reaction mixture is refluxed, 360 ml of 18% hydrochloric acid are added dropwise over a period of 15 to 20 minutes and the mixture is refluxed overnight with stirring. The reaction mixture is cooled to room temperature, filtered and the zinc amalgam is washed with 500 ml methylene chloride. The filtrate is made alkaline with 1 litre of concentrated NH40H and the alkaline phase is extracted once with 1 litre methylene chloride and twice 25 with, each time, 500 ml methylene chloride. The combined organic phases are washed with water,

dried and evaporated. The resultant oil is chromatographied on silicagel using methylene chloride with 2% methanol as eluant to give (±!-(4R*,5aS*)5-ethyl-4,5,5a,6-tetrahydro-9,10-dimethoxy-4-fl-propyl-dibenz[cd,f]indole in form of an oil. <

EXAMPLE 2

30 The (4RS)-5-ethyl-4,5-dihydro-4-hydroxy-9,10-dimethoxy-4-/j-propyl-dibenz[cd,f]indole obtained in the Example 1 b), may also be prepared as follows:

a) 9-amino-3,4-dimethoxy-phenanthrene [compound of formula X]

A mixture of 430 ml (3.08 moles) trifluoroacetic anhydride and 430 ml (5.62 moles)

trifluoroacetic acid is added at room temperature under a nitrogen atmosphere to 53.6 g (0.19 mole) 35 3,4-dimethoxy-phenanthrene-9-carboxylic acid and the mixture is stirred for 10 minutes. After the mixture is cooled to —5°, 15.2 g (0.234 mole) sodium azide are carefully added in solid form, the mixture is stirred for 3 hours at 2°, poured onto ice and the resulting suspension worked up in known manner to give 9-amino-3,4-dimethoxy-phenanthrene as a solid. The hydrochloride melts at over 215° with decomposition.

40 b) 9-acetylamino-3,4-dimethoxy-phenanthrene [compound of formula IX]

55 ml (0.319 mole) N-ethyldiisopropylamine are added to a solution of 38.2 g (0.151 mole) 9-amino-3,4-dimethoxy-phenanthrene in 200 ml methylene chloride. To the resulting mixture is added dropwise over 200 minutes a solution of 20.8 ml (0.292 mole) acetyl chloride in 250 ml methylene chloride. During the addition, the temperature of the reaction mixture is maintained at 20° by cooling 45 with a ice bath. The reaction mixture is stirred for 14 hours at room temperature and worked up in . known manner to afford 9-acetylamino-3,4-dimethoxy-phenanthrene; M.pt. 190—195° after recrystallisation from ether.

c) 9-ethylamino-3,4-dimethoxy-phenanthrene [compound of formula VIII]

34 g (0.124 mole) 9-acetylamino-3,4-dimethoxy-phenanthrene in suspension in 450 ml 50 anhydrous tetrahydrofuran are reduced with 500 ml (0.5 mole) of a molar solution of diborane in anhydrous tetrahydrofuran and worked up in known manner to give 9-ethylamino-3,4-dimethoxy-phenanthrene; M.pt. 94—95°.

d) 4,5-dihydro-9(10-dimethoxy-5-ethyl-4-oxo-dibenz[cd,f]indole [compound of formula VI]

122 ml (0.2 mole) of a 15% solution of /j-butyl-lithium in hexane are added at 0°, under a nitrogen 55 atmosphere, to a solution of 28.1 g (0.1 mole) of 9-ethylamino-3,4-dimethoxy-phenanthrene in 300 ml anhydrous tetrahydrofuran; the reaction mixture becomes bright red. Dry ice is then added until the colouration disappears: the formation of a solution with yellow-green fluorescence is observed. After the temperature of the reaction mixture is allowed to reach room temperature, the mixture is poured

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GB 2 078 225 A 5

onto water/ice( extracted three times with methylene chloride and the organic phase dried over sodium sulfate and evaporated. There is thus obtained 4,5-dihydro-9,10-dimethoxy-5-ethyl-4-oxo-dibenz[cd,f]indole which melts at 125—126° (with decomposition) after crystallisation from ether/petroleum ether.

5 e) (4RS)-5-ethyl-4,5-dihydro-4-hydroxy-9,10-dimethoxy-4-/7-propyl-dibenz[cd,f]indole [compound of 5 formula 111]

2.1 mi (33 mM) of a solution of n-propyl-magnesium bromide in ether are added dropwise at room • temperature to a solution of 10 g (33 mM) of 4,5-dihydro-9,10-dimethoxy-5-ethyl-4-oxo-dibenz[cd,f]indole in 300 ml anhydrous tetrahydrofuran. After 30 minutes, a solution of ammonium 10 chloride is added, the mixture extracted with methylene chloride and the organic phase dried and 10

evaporated to give (4RS)-5-ethyl-4,5-dihydro-4-hydroxy-9,10-dimethoxy-4-n-propyl-dibenz[cd,f]indole in the form of a yellow green foam. [IR Spectrum (CH2CI2): 3540 cm-1 (OH)]. The crude product is directly used for the next step.

EXAMPLE 3

15 (+}-(4R*,5aS*)-5-ethyl-4,5,5a,6-tetrahydro-9,10-dihydroxy-4-n-propyl-dibenz[cd,f]indole 15

20 g (27.66 mM) (+)-(4R*,5aS*)-5-ethyl-4,5,5a,6-tetrahydro-9,10-dimethoxy-4-/?-propyl-dibenz[cd,fjindole in 200 ml of a 47°6 aqueous solution of hydrobromic acid are warmed for 6 hours at reflux, at a bath temperature of 150°. After evaporation of the reaction mixture to dryness, the crystalline residue is stirred in acetone and filtered under suction. The precipitate is washed with 20 acetone then with ether and dried under high vacuum. There is thus obtained (±)-(4R*,5aS*)-5-ethyl- 20 4,5,5a,6-tetrahydro-9,10-dihydroxy-4-/?-propyl-dibenz[cd,f]indole hydrobromide which melts at 200°

with decomposition.

The following compounds may be prepared in analogous manner from the appropriate starting materials:

25 a) (±)-(4R*,5aS*)-4,5-diethyl-4,5,5a,6-tetrahydro-9,10-dihydroxy-dibenz[cd,f]indole 25

(hydrobromide, M.pt >175° with decomposition);

b) (+)-(4R*,5aS*)-4,5,5a,6-tetrahydro-9,10-dihydroxv-4 5-di-/7-propyl-dibenzLcd,fJindole

(hydrobromide, M.pt >190° with decomposition).

c) (+)-{4R*,5aS*)-4-ethyl-4,5,5a,6-tetrahydro-9,10-dihydroxy-5-n-propyl-dibenz[cd,f]indole.

30 EXAMPLE 4 30

(—)-(4S,5aR)-5-ethyl-4,5,5a,6-tetrahydro-9,10-dihydroxy-4-A7-propyl-dibenz[cd,f]indole a) (—M4S,5aR)-5-ethyl-4,5,5a,6-tetrahydro-9,10-dimethoxy-4-/7-propyl-dibenz[cd,f]indole

30 g (89 mM) of (±)-(4R*,5aS*)-5-ethyl-4,5,5a,6-tetrahydro-9,10-dimethoxy-4-n-propyl-dibenz[cd,f]indole are dissolved in 300 ml ether and a solution of 35.95 g of (—)-di-0,0-p-toluoyl-L-35 tartaric acid monohydrate in 300 ml ether is added with stirring. The mixture is further stirred for one 35 hour at room temperature, a total of 1 litre ether being added in portions during this period. The resultant precipitate is filtered off under suction, washed with ethyl acetate until it remains light yellow, and dried.

61.88 g of the crystals obtained from the first crystallisation are dissolved in 1 litre acetone and 40 300 ml methanol at reflux and the solution is filtered and concentrated until a major part of the product 40 crystallizes out. The mixture is stirred for about 15 minutes, the product is filtered off under suction,

washed with ethyl acetate until it remains colourless and dried.

The resulting product is recrystallised in the same manner by using 1.7 litres acetone and 35 ml methanol to give colourless crystals.

45 The resulting crystals are recrystallised in the same manner, by using 1.2 litres acetone and 60 ml 45

methanol. There is thus obtained {—)-(4S,5aR)-5-ethyl-4,5,5a,6-tetrahydro-9,10-dimethoxy-4-n-propyl-dibenz[cd,f]indole (—)-di-0,0-p-toluoyl-L-tartrate in form of colourless crystals which melt at 178—179°; [do0 =—138° (c = 0.29 in methanol).

The following compounds may be prepared in analogous manner from the appropriate starting 50 materials: 50

— (—)-(4S,5aR)-4,5-diethyl-4,5,5a,6-tetrahydro-9,10-dimethoxy-dibenz[cd,f]indole (—)-di-0,0-p-toluoyl-L-tartrate, M.pt 187—189°; [a]£° = —138° (c = 0.5 in methanol); the racemic starting material is reacted first with (+)-di-0,0-p-toluoyl-D-tartaric acid instead of the L-isomer and the mother liquor obtained on crystallisation is treated with NH4OH to liberate the base. The base is reacted with (—)-di-

55 0,0-p-toluoyl-L-tartaric acid and the crystals obtained from an ether solution. 55

— (—)-(4S,5aR)-4,5,5a,6-tetrahydro-9,10-dimethoxy-4,5-di-n-propyl-dibenz[cd,f]indole (—)-di-0,0,-p-toluoyl-L-tartrate, M.pt 165—166°; [a]^0 = —123° (c = 0.27 in methanol).

b) (—)-(4S,5aR)-5-ethyl-4,5,5a,6-tetrahydro-9,10-dihydroxy-4-/7-propyl-dibenz[cd,f]indole

Proceding as described in Example 3, (—)-(4S,5aR)-5-ethyl-4,5,5a,6-tetrahydro-9,10-dihydroxy-60 4-/?-propyl-dibenz[cd,f]indole hydrobromide is obtained from the tartrate obtained above under a). It 60

2078225A I >

6

GB 2 078 225 A 6

melts above 210°; lajg0 = —64° (c = 0.245 in methanol).

The corresponding hydrochloride melts at above 185° with decomposition; [ct]§° = —75° (c = 0.28 in methanol).

EXAMPLE 5

5 The following compounds may be prepared in analogous manner as described in Example 4b)

from the appropriate starting materials:

a) (—)-(4S,5aR)-4,5-diethyl-4,5,5a,6-tetrahydro-9,10-dihydroxy-dibenz[cd,f]indole hydrochloride, M.pt. 200° with decomposition; [arj£° = —72° (c = 0.25 in methanol);

b) (—)-(4S,5aR)-4.5,5a,6-tetrahydro-9,10-dihydroxy-4,5-di-n-propyl-dibenz[cd,f]indole hydrochloride, 10. M.pt > 187° with decomposition; la]J° = —58.5° (c = 0.35 in methanol).

c) (-)-(4S,5aR)-4-ethyl-4,5,5a,6-tetrahydro-9,10-dihydroxy-5-n-propyl-dibenz[cd,f]indole.

EXAMPLE 6

{+)-(4S,5aR)-5-ethyl-9,10-dibenzoyloxy-4,5,5a,6-tetrahydro-4-n-propyl-dibenz[cd,f]indole

0.376 ml (3.23 mM) of benzoyl chloride are added dropwise with stirring, over 5 minutes and at a 15' temperature of +5°, to a solution of 600 mg (1.54 mM) of (—)-(4S,5aR)-5-ethyl-4,5,5a,6-tetrahydro-9,10-dihydroxy-4-/j-propyl-dibenz[cd,f]indole hydrobromide in 5 ml anhydrous pyridine and the reaction mixture is further stirred for 14 hours at room temperature. The mixture is then evaporated to dryness, the residue is dissolved in methylene chloride and the solution is washed first with a mixture of ice and a saturated solution of potassium bicarbonate, and then with water. The aqueous phases are extracted 20 twice with methylene chloride and the combined organic phases are dried and evaporated. The residue is dissolved in methylene chloride and the solution evaporated under high vacuum. The procedure of dissolving the residue in methylene chloride and evaporating is repeated twice more. 550 mg of the resulting product are dissolved in acetone and this solution is added dropwise to a solution of 156 mg L(+)-tartaric acid in acetone. The resultant precipitate is filtered off and dried to give (+)-(4S,5aR)-5-25 ethyl-9,10-dibenzoyloxy-4,5,5a,6-tetrahydro-4-n-propyl-dibenz[cd,f]indole L(+)tartrate; it melts at 161—162° after crystallisation in ethyl acetate/ether; [ar]§4 = +23.5° (c = 0.28 in methanol).

The following compounds may be prepared in analogous manner from the appropriate starting materials

— {—)-(4S,5aR)-9,10-diacetoxy-5-ethyl-4,5,5a,6-tetrahydro-4-/}-propyl-dibenz[cd,f]indoIe 30 hydrochloride, M.pt >188° (with decomposition); [ajg0 = —99° (c = 0.28 in methanol);

— (_)-(4S,5aR)-5-ethyl-4,5,5a,6-tetrahydro-4-/7-propyl-9,10-dipropionyloxy-dibenz[cd,f]indole hydrochloride, M.pt >175° (with decomposition); [a]o° = —76° (c = 0.25 in methanol);

— (—)-(4S,5aR)-9,10-dibutyryloxy-5-ethyl-4,5,5a,6-tetrahydro-4-/7-propyl-dibenz[cdrf]indole hydrochloride, M.pt > 105° (with decomposition); [a]£° = —77° (c = 0.28 in methanol)

35 — (—)-(4S,5aR)-5-ethyl-4,5,5a,6-tetrahydro-9,10-diisobutyryloxy-4-/j-propyl-dibenz[cd,f]indole hydrochloride, M.pt > 165° (with decomposition); = —96° (c = 0.29 in methanol).

— (—)-(4S,5aR)-5-ethyl-4,5,5a,6-tetrahydro-4-/7-propyl-9f10-divalerYloxy-dibenz[cd,f]indoie hydrochloride;

— (—)-(4S,5aR)-5-ethyl-4,5,5a,6-tetrahydro-4-n-propyl-9,10-dipivaloyloxy-dibenz[cd,f]indole 40 hydrochloride;

— (—)-(4S,5aR)-4,5,5a,6-tetrahydro-9,10-dipropionyloxy-4,5-di-/7-propyl-dibenz[cd,f]indole, M.pt 115°; [a]*0 = —76° (c = 0.51 in methanol).

In analogous manner to Example 6, the following compounds of formula I wherein R, and R2 are each independently ethyl or /7-propyl and R3 is

45 —acetoxy

— propionyloxy

— butyryloxy

— isobutyryloxy

— valeryloxy 50 —pivaioyloxy may be prepared.

The compounds of formula 1 possess pharmacological activity. In particular, the compounds are indicated for use as central dopaminergic stimulant agents, as indicated by the following standard tests: The dopaminergic activity of compounds of formula I was studied in the rat according the method 55 described by U. Ungerstedt in Acta Physiol. Scand., Suppl. 367,69—93 (1971). 6-hydroxydopamine is unilaterally injected in the substantia nigra which produces, after a week, unilateral degeneration of nigrostriatal pathways. Administration of from about 0.03 to about 1 mg/kg i.p. of the compounds of formula I to these rats leads to a notable turning behaviour of long duration contralateral to the lesion. The central dopaminergic activity of compounds of formula I has been also confirmed according 60 the following test.

BNSDOCID: <GB 2078225A_I_>

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GB 2 078 225 A 7

Rats, 180—222 g, are placed in "Perspex" (registered Trade Mark) cylinders of 30 cm diameter on a wire grid floor. After 30 minutes to allow acclimatisation to the cage, the rats are injected with the compound under investigation. The behaviour of the rats is observed for 2 minutes at 30 minutes intervals for 2 hours and then at 60 minutes intervals for total of up to 6 hours. The degree of 5 stereotyped behaviour observed is assessed using a scoring system based on that described by Costall, 5 Naylor and Olley [Euro J. Pharmac. 18, 83—94 (1972)].

The scores and criteria are as follows:

1. Intermittent sniffing

2. Persistent sniffing, ocassiona! licking

10 3. Licking, occasional biting 10

4. Intense and persistent biting.

Administered i.p. from about 0.03 to about 30 mg/kg animal body weight, the compounds of formula I induce stereotyped sniffing, licking and biting behaviour in the rat.

The compounds are therefore indicated for use as central dopaminergic stimulant agents, for 15 example for treating Morbus Parkinson. For this indication, an indicated daily dose is from about 0.1 to 15 about 20 mg, conveniently administered in divided doses 2 to 4 times a day in unit dosage form containing from about 0.025 to about 10 mg, or in sustained release form.

The compounds of the invention exhibit furthermore anti-depressant activity, as indicated by the inhibition of catalepsy induced by reserpine in mice on s.c. administration of about 0.001 mg to about 1 20 mg/kg of the compounds and by the inhibition of the catalepsy induced by tetrabenazine in rats on i.p. 20 administration of about 0.2 to about 2 mg/kg of the compounds.

The compounds are therefore indicated for use as anti-depressant agents.

An indicated daily dosage is in the range from about 0.05 to about 2 mg, conveniently given in divided doses 2 to 4 times a day in unit dosage form containing from about 0.01 mg to about 1 mg of 25 the compounds admixed with a solid or liquid pharmaceutical carrier or diluent. 25

The compounds of formula l exhibit furthermore prolactin secretion inhibition as indicated by the lowering of serum levels of prolactin of the rat after s.c. administration of from about 0.1 to about 1 mg/kg of the compounds, using radio-immunological techniques, e.g. described by Niswender, G.D. et a!., Biol. &Med. 130, 793 (1969) and Neil, J.J. etal.. Endocrinology 88, 548 (1971). 30 The compounds of formula I are therefore indicated for use as prolactin inhibitors, e.g. in the 30

treatment of conditions involving hyperprolactinemia, such as menstrual dysfunctions, e.g.

ammenorrhea and galactorrhea, or hypertension of mammary carcinoma associated with elevated serum prolactin levels, or in the treatment of conditions involving hypogonadism, e.g. infertility or impotence, or in the regulation of post-partem lactation.

35 An indicated daily dosage is in the range from about 5 to about 50 mg, conveniently given in 35

divided doses 2 to 4 times a day in unit dosage form containing from about 1.25 mg to about 25 mg of the compounds admixed with a solid or liquid pharmaceutical carrier or diluent.

The compounds of the invention exhibit furthermore antipsychotic activity as indicate by the inhibition of the locomotion in mice on s.c. administration of from about 0.001 mg to about 0.1 mg/kg 40 of the compounds and by the dopamine agonistic action on the presynaptic receptors of the rat at doses 40 of from about 1 mg to about 10 mg/kg of the compounds, according the following test.

The dopamine agonistic action of the compounds of formula I on the presynaptic receptors was studied on the rat using the in vivo method described by J. R. Walters and coll. in Naunyn Schmiedeberg's Arch. Pharmacol. 296, 5—14 (1976). The dopaminergic impulse flow was inhibited 45 pharmacologically by p-butyrolactone. The activity of the aromatic amino acid decarboxylase was 45

inhibited by hydroxybenzylhydrazine (NSD 1015) and half an hour later the rats were sacrificed. The resulting accumulation of DOPA during 30 minutes in striatal tissue was taken as a measure of the in vivo activity of tyrosine hydroxylase. Administration per os of the compounds reversed the effects of y-butyrolactone in a dose dependent manner.

50 The compounds of formula I are therefore indicated for use as antipsychotic agents, for example 50 for the treatment of schizophrenia. An indicated daiiy dosage is in the range from about 0.01 to about 1 mg, conveniently given in divided doses 2 or 4 times a day in unit dosage form containing from about 0.0025 mg to about 0.5 mg of the compounds admixed with a solid or liquid pharmaceutical carrier or diluent.

55 The preferred indication is the anti-parkinson indication. 55

The compounds (4S,5aR)-5-ethyl-4,5,5a,6-tetrahydro-9,10-dihydroxy-4-/7-propyl-dibenz[cd,f]indole, (4S,5aR)-4-ethyl-4,5,5a,6-tetrahydro-9,10-dihydroxy-5-/7-propyl-9,10-dihydroxy-dibenz[cd,f]indole, (4S,5aR)r4,5-diethyl-4,5,5a,6-tetrahydro-9,10-dihydroxy-dibenz[cd,f]indole and (4S,5aR)-4,5,5ar6-tetrahydro-9,10-dihydroxy-4,5-di-/?-propyl-dibenz[cd,f]indole and the corresponding 60 di-n-propionyl- and di-isobutyrylester-derivatives are the preferred compounds. 60

The compounds of formula I may be administered in pharmaceutically acceptable acid addition salt form. These salt forms exhibit the same order of activity as the free base forms.

The present invention also provides a pharmaceutical composition comprising a compound of

2078225A I >

8

GB 2 078 225 A 8

formula f, in free base form or in pharmaceutical^ acceptable acid addition salt form, in association with a pharmaceutically acceptable diluent or carrier.

These compositions may be formulated in conventional manner so as to be, for example, a solution, a capsule or a tablet.

Claims

5 CLAIMS

1. A process for the production of a compounds of formula I,

wherein

R, and R2 independently, are ethyl or /j-propyi radicals, and 10 the R3 substituents are the same and are hydroxy or acyloxy radicals, 1 q in racemic form having the relative configuration 4R*,5aS*, or in optically active isomer form having the absolute configuration 4S,5aR, which comprises a) producing a compound of formula la'

la

15. wherein R, and R2 are defined above and Z is a splittable ether group, in racemic form having the 15

wherein R, and R2 are defined above and Z is a splittable ether group, in racemid form having the relative configuration 4R*,5aS*, or in optically active isomer form having the absolute configuration 4S,5aR, or

20 b) producing a compound of formula lb

20

lb wherein R, and R2 are defined above and the radicals R3 are identical acyloxy radicals, by acylating a compound of formula la in racemic form having the relative configuration 4R",5aS* or in optically active isomer form having the absolute configuration 4S,5aR.

25 2. A process for the production of a compound of formula I defined in Claim 1 substantially as 25 hereinbefore described with reference to any one of the examples 3 to 6.

3. A compound of formula I whenever produced by a process as claimed in Claim 1.

4. A compound of formula I as defined in Claim 1.

5. A compound of Claim 4 wherein each R3 is hydroxy.

30 6. A compound of Claim 4 wherein each R3 is acyloxy. 30

7. A compound of Claim 6 wherein each acyloxy radical is of formula

Ra—CO—O-

wherein Ra is an alkyl radical, (C3_7)cycIoalkyl, a phenyl radical or a 5- or 6-membered heterocyclic ring. 8. A compound of Claim 7 wherein RaC00-— is propionyioxy.

35 9. A compound of Claim 7 wherein RaCOO—is isobutyryloxy.

10. A compound of any one of Claims 4 to 9 wherein R, and R2 are each ethyl.

11. A compound of any one of Claims 4 to 9 wherein R, and R, are each n-propyl.

35

BNSDOCID: <GB 2078225A_L>

9

GB 2 078 225 A 9

12. A compound of any one of Claims 4 to 9 wherein R1 is ethyl and R2 is /7-propyf.

13. A compound of any one of Claims 4 to 9 wherein R, is /7-propyi and R2 is ethyl.

14. A compound of any one of Claims 4 to 13 in racemic form having the relative configuration 4R*,5aS*.

5 15. A compound of any one of Claims 4 to 13 in optically active isomer form having the absolute 5 configuration 4S,5aR.

16. (±)-{4R*,5aS*)-5-ethyl-4,5,5a,6-tetrahydro-9,10-dihydroxy-4-n-propyl-dibenz[cd,f]indole.

17. (±H4R*,5aS*)-4,5-diethyl-4,5,5a,6-tetrahydro-9,10-dihydroxy-dibenz[cd,f]indole.

18. (±M4R*,5aS*)-4,5,5a,6-tetrahydro-9,10-dihydroxy-4,5-di-/7-propyl-dibenz[cd,f]indole.

10 19. (±)-(4R*,5aS*)-4-ethyl-4,5,5a,6-tetrahydro-9,10-dihydroxy-5-/?-propyl-dibenz[cd,f]indole 10

20. (4S,5aR)-5-ethyl-4,5,5a,6-tetrahydro-9,10-dihydroxy-4-/7-propyl-dibenzlcd,f]indole.

21. (4S,5aR)-4-ethyl-4,5,5a,6-tetrahydro-9,10-dihydroxy-4-/7-propyl-dibenz[cd,f]indole.

22. (4S,5aR)-4,5-diethyl-4,5,5a,6-tetrahydro-9,10-dihydroxy-dibenz[cd,f]indole.

23. (4S,5aR)-4,5,5a,6-tetrahydro-9,10-dihydroxy-4,5-di-/7-propyl-dibenz[cd,f]indole.

15 24. (4S,5aR)-5-ethyl-9,10-dibenzoyloxy-4,5,5a,6-tetrahydro-4-/7-propyl-dibenz[cd,fjindole. 15

25. (4S,5aR)-9,10-diacetoxy-5-ethyl-4,5,5af6-tetrahydro-4-/?-pr9pyl-dibenzlcd/fJindole.

26. (4S,5aR)-5-ethyl-4,5,5a,6-tetrahydro-4-/7-propyl-9,10-dipropionyloxy-dibenz[cd,f]indole.

27. (4S,5aR)-9,10-dibutyryloxy-5-ethyl-4,5,5a,6-tetrahydro-4-n-propyl-dibenz[cd,f]indoIe.

28. (4S,5aR)-5-ethyl-4,5,5a,6-tetrahydro-9,10-diisobutyryIoxy-4-/7-propyl-dibenz[cd,f]indole.

20 29. (4S,5aR)-4,5,5a,6-tetrahydro-9,10-dipropionyloxy-4,5-di-/?-propyl-dibenz[cd,f]indole. 20

30. A compound of any one of Claims 4 to 29 in free base form.

31. A compound of any one of Claims 4 to 29 in acid addition salt form.

32. A pharmaceutical composition which comprises a compound of any one of claims 4 to 29, in free base form or in pharmaceutical^ acceptable acid addition salt form, in association with a

25 pharmaceutical carrier or diluent. 25

Printed for Her Majesty's Stationery Office by the Courier Press, Leamington Spa, 198Z Published by the Patent Office, 25 Southampton Buildings, London, WC2A 1AY, from which copies may be obtained.

BNSDOCID: <GB 2078225A_I_>