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CU20120055A7 - Método para la detección, recuperación, identificación y enumeración simultánea de microorganismos - Google Patents

Método para la detección, recuperación, identificación y enumeración simultánea de microorganismos

Info

Publication number
CU20120055A7
CU20120055A7 CUP2012000055A CU20120055A CU20120055A7 CU 20120055 A7 CU20120055 A7 CU 20120055A7 CU P2012000055 A CUP2012000055 A CU P2012000055A CU 20120055 A CU20120055 A CU 20120055A CU 20120055 A7 CU20120055 A7 CU 20120055A7
Authority
CU
Cuba
Prior art keywords
recovery
identification
microorganisms
detection
simultaneous
Prior art date
Application number
CUP2012000055A
Other languages
English (en)
Other versions
CU24137B1 (es
Inventor
Martínez Claudio Rodríguez
Ruíz Jesús Eduardo González
Rodríguez Tamara Lobaina
Raisa Zhurbenko
González Ana Iris Brito
Hernández Mónica López
Fernández Javier Aragón
Valdés Ivonne Alfonso
Surís Adelaida Ortega
Original Assignee
Ct Nac Biopreparados
Cnic Ct Nac Investigaciones
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ct Nac Biopreparados, Cnic Ct Nac Investigaciones filed Critical Ct Nac Biopreparados
Priority to CU20120055A priority Critical patent/CU24137B1/es
Priority to ES13767434.7T priority patent/ES2650453T3/es
Priority to EP13767434.7A priority patent/EP2837690B1/en
Priority to CA2883197A priority patent/CA2883197C/en
Priority to BR112014024361-1A priority patent/BR112014024361B1/pt
Priority to US14/389,568 priority patent/US20150148258A1/en
Priority to RU2014143767A priority patent/RU2644683C2/ru
Priority to PCT/CU2013/000002 priority patent/WO2013143508A2/es
Priority to MX2014011804A priority patent/MX2014011804A/es
Publication of CU20120055A7 publication Critical patent/CU20120055A7/es
Publication of CU24137B1 publication Critical patent/CU24137B1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/14—Scaffolds; Matrices
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/36—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of biomass, e.g. colony counters or by turbidity measurements
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C12Q1/06—Quantitative determination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C12Q1/10—Enterobacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C12Q1/14—Streptococcus; Staphylococcus
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
    • G01N2333/21—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Pseudomonadaceae (F)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
    • G01N2333/24—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • G01N2333/245—Escherichia (G)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
    • G01N2333/315—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Streptococcus (G), e.g. Enterococci
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/37—Assays involving biological materials from specific organisms or of a specific nature from fungi
    • G01N2333/38—Assays involving biological materials from specific organisms or of a specific nature from fungi from Aspergillus
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/37—Assays involving biological materials from specific organisms or of a specific nature from fungi
    • G01N2333/39—Assays involving biological materials from specific organisms or of a specific nature from fungi from yeasts
    • G01N2333/40—Assays involving biological materials from specific organisms or of a specific nature from fungi from yeasts from Candida

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Toxicology (AREA)
  • Biomedical Technology (AREA)
  • Sustainable Development (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

La presente invención describe un método y dispositivos para la detección, recuperación, identificación y enumeración simultánea de una pluralidad de microorganismos consistente en proveer mezclas de sustancias nutritivas especialmente seleccionadas entre aquellas que acortan la fase lag de crecimiento de bacterias y mohos y que, conjuntamente con marcadores enzimáticos fluorescentes, cromogénicos o bioluminiscentes y otros componentes nutritivos o inhibidores del crecimiento, son embebidos en estructuras tridimensionales de arcillas o cerámicas naturales o artificiales con cavidades de diferentes dimensiones y formas y superficies específicas entre 2 x 10?3 y 6 x 108 m2/m3.
CU20120055A 2012-03-30 2012-03-30 Método para la detección, recuperación, identificación y enumeración simultánea de microorganismos CU24137B1 (es)

Priority Applications (9)

Application Number Priority Date Filing Date Title
CU20120055A CU24137B1 (es) 2012-03-30 2012-03-30 Método para la detección, recuperación, identificación y enumeración simultánea de microorganismos
ES13767434.7T ES2650453T3 (es) 2012-03-30 2013-03-27 Método para la detección, recuperación, identificación y conteo simultáneos de bacterias y hongos, y disposición de estructura tridimensional para la implementación de dicho método
EP13767434.7A EP2837690B1 (en) 2012-03-30 2013-03-27 Method for simultaneous detection, recovery, identification and counting of microorganisms and three dimentioanl structure for the implementation of said method
CA2883197A CA2883197C (en) 2012-03-30 2013-03-27 Method for simultaneous detection, recovery, identification and counting of microorganisms and devices for the implementation of said method
BR112014024361-1A BR112014024361B1 (pt) 2012-03-30 2013-03-27 Método para a detecção, a recuperação, a identificação e a contagem simultâneas de micro-organismos e dispositivos para a sua implementação
US14/389,568 US20150148258A1 (en) 2012-03-30 2013-03-27 Method for simultaneous detection, recovery, identification and counting of microorganisms and devices for the implementation of said method
RU2014143767A RU2644683C2 (ru) 2012-03-30 2013-03-27 Способ получения трехмерных структур, используемых для детекции, выделения или подсчета микроорганизмов
PCT/CU2013/000002 WO2013143508A2 (es) 2012-03-30 2013-03-27 Método para la detección, recuperación, identificación y enumeración simultánea de microorganismos y dispositivos para su ejecución
MX2014011804A MX2014011804A (es) 2012-03-30 2013-03-27 Metodo para la deteccion, recuperacion, identificacion y enumeracion simultanea de microorganismos y dispositivos para su ejecucion.

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CU20120055A CU24137B1 (es) 2012-03-30 2012-03-30 Método para la detección, recuperación, identificación y enumeración simultánea de microorganismos

Publications (2)

Publication Number Publication Date
CU20120055A7 true CU20120055A7 (es) 2013-12-27
CU24137B1 CU24137B1 (es) 2015-12-23

Family

ID=49261336

Family Applications (1)

Application Number Title Priority Date Filing Date
CU20120055A CU24137B1 (es) 2012-03-30 2012-03-30 Método para la detección, recuperación, identificación y enumeración simultánea de microorganismos

Country Status (9)

Country Link
US (1) US20150148258A1 (es)
EP (1) EP2837690B1 (es)
BR (1) BR112014024361B1 (es)
CA (1) CA2883197C (es)
CU (1) CU24137B1 (es)
ES (1) ES2650453T3 (es)
MX (1) MX2014011804A (es)
RU (1) RU2644683C2 (es)
WO (1) WO2013143508A2 (es)

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WO2016077293A1 (en) * 2014-11-10 2016-05-19 Charm Sciences, Inc. Culture peel plate
US10344253B2 (en) 2014-11-25 2019-07-09 3M Innovative Properties Company Devices and kits for the propagation or storage of microorganisms, and methods of making and using
RU2636620C1 (ru) * 2016-10-25 2017-11-24 Федеральное государственное бюджетное учреждение науки "Тюменский научный центр Сибирского отделения РАН" (ТюмНЦ СО РАН) Способ оптической оценки концентрации микробных клеток в суспензии
CN109182445B (zh) * 2018-09-20 2021-10-22 吉林农业大学 一种霉菌和酵母计数测试片、制备方法及其应用
CN113039262B (zh) * 2018-10-30 2024-02-06 科莱鹤株式会社 用于捕捉细胞外囊泡的器件、细胞外囊泡的保存方法和转送方法
CN110257468B (zh) * 2019-06-03 2022-05-27 北京鑫骥金诺医疗器械有限公司 一种用于细菌计数的质控的组合物、制备方法及其应用
CN112795486B (zh) * 2020-12-30 2023-09-19 兴源环境科技股份有限公司 一种畜禽粪污微藻养殖同步产乙醇的方法
CN114563396A (zh) * 2022-01-26 2022-05-31 湖南农业大学 一种水中原核细胞型微生物总数现场快速检测试剂盒和检测方法

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Also Published As

Publication number Publication date
EP2837690A4 (en) 2015-11-25
CU24137B1 (es) 2015-12-23
WO2013143508A3 (es) 2014-05-08
EP2837690B1 (en) 2017-09-13
EP2837690A2 (en) 2015-02-18
WO2013143508A2 (es) 2013-10-03
US20150148258A1 (en) 2015-05-28
MX2014011804A (es) 2015-03-19
CA2883197A1 (en) 2013-10-03
ES2650453T3 (es) 2018-01-18
BR112014024361A2 (pt) 2017-07-25
CA2883197C (en) 2020-03-31
RU2014143767A (ru) 2016-05-27
BR112014024361A8 (pt) 2022-03-08
RU2644683C2 (ru) 2018-02-13
BR112014024361B1 (pt) 2022-06-07

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