CS270801B1 - Method of lysine or tylosine containing stable bioconcentrates preparation - Google Patents

Abstract

This presented innovation concerns to a method of preparation of stable bioconcentrates containing lysine and/or tylosin where satisfactory physical, physico-chemical, and stabilizing parameters of a product are achieved by a dosage of hydrophobized calcium carbonate into the drying area during the spray-drying process. Use of the subject of this solution is assumed during the production of technical products with the content of lysine and tylosin being further processed into biofactor supplements or medicated fodder and crops for livestock.

Description

The invention relates to a process for preparing stable bioconcentrates containing lysine or tylosin.

At present, essential amino acids, in addition to vitamins, are the most commonly used substances in the nutrition of agricultural, utility animals in the form of stabilized bioconcentrates as biofactor supplements, possibly domedicated feed preparations and compound feeds. They are prepared predominantly by fermentation and subsequent isolation either by crystallization from purified solutions or by spray-drying of concentrated biomass to technical products. Spray-dried fermented, more or less thickened biomass products are very economically and biologically advantageous, since energy, time-consuming and laborious isolation and associated losses of the active substance are eliminated, and the material present in the concentrate is bioavailable, as it consists of coagulated protein walls of microorganisms from fermentation broth. A considerable disadvantage of such products is the pronounced hygroscopicity, tackiness and hence their stability. In a short time after drying, particle aggregation, increased surface moisture and a risk of microbial contamination of the dried biomass will occur.

Several methods have been used to reduce the hygroscopicity of dusts, crystals and granules. Most often, the film coating of the particles with the film former is dissolved in an organic solvent. Technology is known in conventional pharmaceutical practice (E. L. Parrott: Pharmaceutical Technology, Burgess Publ. Compl, Minneapolis USA, 1970). The disadvantage is a complicated process, higher production costs, increased risk of explosion hazard and endangering the health of the operating personnel. Film coating also reduces the bioavailability of nutrients due to the uneven thickness of the applied film-forming substance. '

The present invention addresses the above drawbacks in the preparation of dried lysine-tylosin-containing technical products by admixing a hydrophobic material in powder form which encapsulates the hygroscopic hydrophilic particles of the dried bioconcentrate during the biomass spraying. The hydrophobic film thus formed prevents the particles from sticking together, the product being free-flowing and more stable to external influences. A carrier base of the inorganic compound may be used to better distribute the hydrophobic material.

Calcium carbonate has proven to be the most suitable carrier, which can be floated, precipitated or micronized with a maximum particle size of up to 100 µm. The surface treatment of the fine calcium carbonate is made by the physical mixing method of a strong hydrophobic substance with a large surface area or by simultaneous grinding of the calcium carbonate with a hydrophobic substance with a lower melting point which uniformly encapsulates the inorganic carrier. Hydrophobic substances must be harmless to health.

In the preparation of a hydrophobic inorganic carrier, such as by mechanical mixing, hydrophobic silica (e.g., Sipermat 17, Aerosil R 972 and the like) may be advantageously used, or the inorganic carrier may be hydrophobized with hydrophobic substances with a lower melting point by grinding it heat. *

SUMMARY OF THE INVENTION The invention is based on the addition of hydrophobized calcium carbonate to an atomized concentrated biomass in an amount of 10 to 30% by weight, containing lysine or tylosin during the drying process, at a temperature of 85 to 110 ° C.

The loosening of the treated, hydrophobized technical lysine was tested by the Klein method (Klein, K., Seifen-01e-Fette-Wachse, 94, 12, 1968). In this method, the masses are evaluated by factors ranging from 1 to 6, where the lower number always indicates the higher and more preferably the flow properties of the test substance.

CS 270 801 B1

In the experiments we used amino acids such as e.g. technical lysine, antibiotics such as e.g. tylosin, bacitracin, which are very difficult to process into a dry form, but the process we present is also applicable to other biologically active materials of technical quality, i. in cases where the active substance is dried directly together with the biomass.

Technical lysine, untreated with a hydrophobic inorganic carrier, was not evaluated by the above method, as a solid aggregate was formed for several minutes when stored on a Petri dish at a relative humidity of 80% at 25 ° C.

In the model experiment, fermented lysine (42% solids content) was used and as a hydrophobic carrier calcium carbonate hydrophobized with hydrophobic silica and in the second case with stearin.

Under the same conditions, stored lysine technical homogenates with close concentrations of hydrophobized calcium carbonate exhibited the following values:

A

Concentration of hydrophobized calcium carbonate (% by weight) (10 4 wt.

Sipermat 0) В Concentration of hydrophobized carbonate . ¹ . 3 5 ¹⁰ . calcium (wt.%) (treatment with 1 wt. stearin) 1. 3 5 ¹⁰ . Klein factor · -. χ: In A 6 5 3 1 В 6 5 2 1 In the following, the subject matter of the invention is illustrated in three without examples that, to on not obmedzo-

val:.

Example 1.

The fermented, concentrated biomass with an L-lysine content of 62 kg.m ^- 1 is fed to the spray dryer at a rate of 34.6 kg.h ^-1 at 210 ° C inlet and 106 ° C at outlet. At the same time, hydrophobised calcium carbonate is sprayed in a counter-direction through the nozzle at a rate of 1.4 kg.h- ^{1 with} non-toxic hydrophobic silica. The calcium carbonate was hydrophobised by mixing with a hydrophobic cream of 10 µl in a homogenizer. The prepared, stable, technical grade bioconcentrate with an L-lysine content of 40 to 43% by weight is adjusted by conventional means.

Example 2. '

After fermentation, the biomass containing 7% by weight of the macrolide anitobiotics tylosin is atomized in a spray dryer at an inlet temperature of 195 ° C, 102 ° C at an outlet at a rate of 23 kg.h ¹ . Hydrophobic calcium carbonate in a ratio of 100: 8 at the rate of 0.32 kg.h -1 is simultaneously added to the drying space through the nozzle in the opposite direction by hydrophobic silica. The prepared stable technical bioconcentrate containing the antibiotic tylosin is adjusted in a conventional manner before further processing into compound feed for veterinary use.

CS 270 801 B1

Example 3

The fermented, concentrated biomass with L-lysine content of 60 kg.m · ⁵ is fed to the spray dryer at a rate of 35 kg.h-1 at the inlet temperature of 210 ° C, 106 ° C at the outlet, and sprayed with stearin in the opposite direction hydrophobised calcium carbonate at a ratio of 1: 99 at a rate of 2.8 kg.h -1. The prepared technical bioconcentrate containing L-lysine is adjusted in a conventional manner.

Claims (3)

SUMMARY OF THE INVENTION A process for the preparation of stable, atomized, dried lysine or tylosin-containing bioconcentrates while feeding an inorganic carrier into a drying chamber, characterized in that a hydrophobized inorganic carrier, e.g. calcium carbonate hydrophobized with hydrophobic silica, stearin or its salts to atomized concentrated biomass in an amount of 10 to 30% by weight, calculated on the amount of dried product, at a temperature of 85 to 210 ° C. 2. The process according to claim 1, wherein the hydrobromic silica is used in an amount of 0.5 to 3% by weight, based on the amount of the inorganic carrier. 3. Process according to claim 1, characterized in that the stearin or its salts are used in an amount of 5 to 10% by weight, calculated on the amount of carrier.