CO4480059A1 - PROCEDURE TO PURIFY A NEW ENZYME: PHTHALYL AMIDASE - Google Patents
PROCEDURE TO PURIFY A NEW ENZYME: PHTHALYL AMIDASEInfo
- Publication number
- CO4480059A1 CO4480059A1 CO95031239A CO95031239A CO4480059A1 CO 4480059 A1 CO4480059 A1 CO 4480059A1 CO 95031239 A CO95031239 A CO 95031239A CO 95031239 A CO95031239 A CO 95031239A CO 4480059 A1 CO4480059 A1 CO 4480059A1
- Authority
- CO
- Colombia
- Prior art keywords
- approximately
- elute
- kcl
- followed
- stage
- Prior art date
Links
- 108030006347 Phthalyl amidases Proteins 0.000 title abstract 2
- 238000000034 method Methods 0.000 title abstract 2
- 108090000790 Enzymes Proteins 0.000 title 1
- 102000004190 Enzymes Human genes 0.000 title 1
- 108090000623 proteins and genes Proteins 0.000 abstract 4
- 102000004169 proteins and genes Human genes 0.000 abstract 4
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 abstract 3
- 229910052921 ammonium sulfate Inorganic materials 0.000 abstract 3
- 239000001166 ammonium sulphate Substances 0.000 abstract 3
- 235000011130 ammonium sulphate Nutrition 0.000 abstract 3
- LWIHDJKSTIGBAC-UHFFFAOYSA-K tripotassium phosphate Chemical compound [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 abstract 3
- 241000448280 Elates Species 0.000 abstract 2
- 239000003957 anion exchange resin Substances 0.000 abstract 2
- 239000008187 granular material Substances 0.000 abstract 2
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 abstract 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-M hydroxide Chemical class [OH-] XLYOFNOQVPJJNP-UHFFFAOYSA-M 0.000 abstract 1
- 230000003993 interaction Effects 0.000 abstract 1
- 229910000160 potassium phosphate Inorganic materials 0.000 abstract 1
- 239000008057 potassium phosphate buffer Substances 0.000 abstract 1
- 235000011009 potassium phosphates Nutrition 0.000 abstract 1
- 239000011347 resin Substances 0.000 abstract 1
- 229920005989 resin Polymers 0.000 abstract 1
- 229910021653 sulphate ion Inorganic materials 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
- C12N15/76—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Actinomyces; for Streptomyces
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
REIVINDICACION NUMERO UN PROCEDIMIENTO PARA PURIFICAR AMIDASA DE FTALILO QUE COMPRENDE: A) CROMATOGRAFIAR UN EXTRACTO BRUTO LIBRE DE CELU- LAS DE AMIDASA DE FTALILO, OBTENIDO MEDIANTE DIS- RUPCION DE CELULAS QUE CONTIENEN AMIDASA DE FTALI- LO, OBTENIDO MEDIANTE LA DISRUPCION DE CELULAS QUE CONTIENEN AMIDADASA DE FTALILO SOBRE UNA RESINA DE INTERCAMBIO DE ANIONES, ELUIR PROTEINAS LIGADAS CON UN GRADIENTE LINEAL DE KCL DE 0-1.5 M, SEGUIDO POR AGRUPAR LAS FRACCIONES ELUIDAS EN KCL ENTRE APROXIMADAMENTE 1M Y APROXIMADAMENTE 1,1 M; B) FRACCIONAR EL ELUADO AGRUPADO DE LA ETAPA A CON SULFATO DE AMONIO Y SOLUBILIZAR GRANULOS OBTENIDOS EN UNA CONCENTRACION DE SULFATO DE AMONIO DE ENTRE APROXIMADAMENTE 67% Y APROXIMADAMENTE 97%; C) CROMATOGRAFIAR LOS GRANULOS SOLUBILIZADOS DE LA ETAPA B SOBRE UNA RESINA DE INTERACCION HIDROFOBA, ELUIR PROTEINAS UNIDAS CON UN GRADIENTE LINEAL DE- CRECIENTE DE SULFATO DE AMONIO 2,6-0 M, SEGUIDO POR AGRUPAR LAS FRACCIONES ELUIDAS EN SULFATO DE AMONIO ENTRE APROXIMADAMENTE 0,4 M Y 0 M Y ELIMI- NAR LAS SALES CONTENIDAS EN EL ELUADO AGRUPADO; D) CROMATOGRAFIAR EN ELUADO AGRUPO DE LA ETAPA C SOBRE HIDROXI LA PATITA ELUIR PROTEINAS UNIDAS CON UN GRADIENTE LINEAL DE FOSFATO DE POTASIO 50-500MM , PH 8,0 SEGUIDO POR AGRUPAR LAS FRACCIONES ELUI- DAS EN FOSFATO DE POTASIO ENTRE APROXIMADAMENTE 150 Y APROXIMADAMENTE 190 MM; Y E) CROMATOGRAFIAR EL ELUADO AGRUPADO DE LA ETAPA D SOBRE UNA RESINA DE INTERCAMBIO DE ANIONES, ELUIR PROTEINAS UNIDAS CON UN GRADIENTE LINEAL DE KCL 0-15 M, SEGUIDO POR AGRUPAR LAS FRACCIONES ELUIDAS EN KCL ENTRE APROXIMADAMENTE 0,72 M Y APROXIMADA- MENTE 0,8 M; EN EL QUE LAS ETAPAS A,B,C,D, Y E SE REALIZAN A UNA TEMPERATURA ENTRE APROXIMADAMENTE 0 GRADOS CENTIGRADOS Y APROXIMADAMENTE 20 GRADOS CENTIGRA- DOS; Y EN EL QUE CADA UNA DE LAS ETAPAS A, B, C, D Y E SE REALIZAN EN TAMPON DE FOSFATO DE POTASIO 50 MM, PH 8,0. FIGURA 1CLAIMS IS A PROCEDURE FOR PURIFYING PHTHALYL AMIDASE, WHICH INCLUDES: THEY CONTAIN AMPHALYL AMPHALYLATE ON AN ANION EXCHANGE RESIN, ELUTE LINKED PROTEINS WITH A LINEAR GRADIENT OF 0-1.5 M KCL, FOLLOWED BY GROUPING THE ELACTION FRACTIONS IN KCL BETWEEN APPROXIMATELY 1.1 M; B) FRACTIONATE THE GROUPED ELATE OF STAGE A WITH AMMONIUM SULPHATE AND SOLUBILIZE GRANULES OBTAINED IN A CONCENTRATION OF AMMONIUM SULPHATE BETWEEN APPROXIMATELY 67% AND APPROXIMATELY 97%; C) CHROMATOGRAPH THE SOLUBLIZED GRANULES OF STAGE B ON A HYDROPHOBA INTERACTION RESIN, ELUTE UNITED PROTEINS WITH A LINEAR DEGRADE OF AMMONIUM SULPHATE 2.6-0 M, FOLLOWED BY GROUPING THE ELUIDED FRACTIONS IN SULPHATE AMULPHATE 0.4 MY 0 MY ELIMINATE THE SALTS CONTAINED IN THE GROUPED ELUATE; D) CHROMATOGRAPH IN ELUATE GROUP OF STAGE C ON HYDROXY LA PATITA ELUTE UNITED PROTEINS WITH A LINEAR GRADIENT OF POTASSIUM PHOSPHATE 50-500MM, PH 8.0 FOLLOWED BY AGRUPAR THE ELUI-FRIDGES IN POTASIUM PHOSPHATE IN POTASIUM 190 MM; YE) CHROMATOGRAPH THE GROUPED ELATE OF STAGE D ON AN ANION EXCHANGE RESIN, ELUTE UNITED PROTEINS WITH A LINEAR GRADIENT OF KCL 0-15 M, FOLLOWED BY GROUPING THE ELACTIONED FRACTIONS IN KCL BETWEEN APPROXIMATELY 0.20 M AND APPROXIMATELY , 8 M; IN WHICH STAGES A, B, C, D, AND E ARE PERFORMED AT A TEMPERATURE BETWEEN APPROXIMATELY 0 DEGREES DEGREE AND APPROXIMATELY 20 DEGREES DEGREE; AND IN WHICH EACH OF THE STAGES A, B, C, D AND E ARE MADE IN POTASSIUM PHOSPHATE BUFFER 50 MM, PH 8.0. FIGURE 1
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US27548994A | 1994-07-15 | 1994-07-15 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CO4480059A1 true CO4480059A1 (en) | 1997-07-09 |
Family
ID=23052522
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CO95031239A CO4480059A1 (en) | 1994-07-15 | 1995-07-14 | PROCEDURE TO PURIFY A NEW ENZYME: PHTHALYL AMIDASE |
Country Status (5)
| Country | Link |
|---|---|
| AU (1) | AU3135595A (en) |
| CO (1) | CO4480059A1 (en) |
| PE (1) | PE32396A1 (en) |
| WO (1) | WO1996002630A1 (en) |
| ZA (1) | ZA955898B (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2000037486A1 (en) * | 1998-12-22 | 2000-06-29 | Holland Sweetener Company V.O.F. | Synthesis and recovery of aspartame involving enzymatic deformylation step |
| US6617127B2 (en) | 1998-12-22 | 2003-09-09 | Holland Sweetener Company, V.O.F. | Synthesis and recovery of aspartame involving enzymatic deformylation step |
| AU6049000A (en) * | 1999-07-23 | 2001-02-13 | Eli Lilly And Company | Enantioselective acylation of cis racemic azetidinones |
| CN109468347A (en) * | 2018-08-30 | 2019-03-15 | 湖南师范大学 | A method for biocatalytic synthesis of optically active 2R-fluorocarboxylic acids and 2R-hydroxycarboxylic acids |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS52148678A (en) * | 1976-06-02 | 1977-12-10 | Agency Of Ind Science & Technol | Microbial decomposition of phthalic acid esters |
| JPS5381681A (en) * | 1976-12-25 | 1978-07-19 | Agency Of Ind Science & Technol | Bio-reaction process |
| US5455959A (en) * | 1992-03-02 | 1995-10-03 | Alcatel Network Systems, Inc. | System for collecting from masters information independently collected from associated slaves in shelves of a telecommunications terminal |
| US5451522A (en) * | 1994-07-15 | 1995-09-19 | Eli Lilly And Company | Genes encoding and method of expressing an enzyme: phthalyl amidase |
-
1995
- 1995-07-14 PE PE1995273858A patent/PE32396A1/en not_active Application Discontinuation
- 1995-07-14 ZA ZA955898A patent/ZA955898B/en unknown
- 1995-07-14 CO CO95031239A patent/CO4480059A1/en unknown
- 1995-07-14 AU AU31355/95A patent/AU3135595A/en not_active Abandoned
- 1995-07-14 WO PCT/US1995/009082 patent/WO1996002630A1/en not_active Ceased
Also Published As
| Publication number | Publication date |
|---|---|
| WO1996002630A1 (en) | 1996-02-01 |
| ZA955898B (en) | 1997-01-14 |
| PE32396A1 (en) | 1996-08-07 |
| AU3135595A (en) | 1996-02-16 |
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