CN2911668Y - Monomolecular controlled traverse magnetic tweezers - Google Patents
Monomolecular controlled traverse magnetic tweezers Download PDFInfo
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- CN2911668Y CN2911668Y CN 200620117879 CN200620117879U CN2911668Y CN 2911668 Y CN2911668 Y CN 2911668Y CN 200620117879 CN200620117879 CN 200620117879 CN 200620117879 U CN200620117879 U CN 200620117879U CN 2911668 Y CN2911668 Y CN 2911668Y
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- magnetic tweezers
- tweezers
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- 230000005291 magnetic effect Effects 0.000 title claims abstract description 77
- 238000003384 imaging method Methods 0.000 claims abstract description 20
- 238000012545 processing Methods 0.000 claims abstract description 4
- 230000005298 paramagnetic effect Effects 0.000 claims abstract 2
- 239000000696 magnetic material Substances 0.000 claims description 9
- 238000011160 research Methods 0.000 abstract description 4
- 102000004190 Enzymes Human genes 0.000 abstract description 3
- 108090000790 Enzymes Proteins 0.000 abstract description 3
- 238000004458 analytical method Methods 0.000 abstract description 2
- 230000009286 beneficial effect Effects 0.000 abstract description 2
- 108090000623 proteins and genes Proteins 0.000 abstract description 2
- 102000004169 proteins and genes Human genes 0.000 abstract description 2
- 210000004027 cell Anatomy 0.000 description 17
- 238000005259 measurement Methods 0.000 description 5
- 239000011324 bead Substances 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 238000010586 diagram Methods 0.000 description 3
- 230000005408 paramagnetism Effects 0.000 description 3
- 238000013519 translation Methods 0.000 description 3
- 102000029749 Microtubule Human genes 0.000 description 2
- 108091022875 Microtubule Proteins 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 238000005286 illumination Methods 0.000 description 2
- 210000004688 microtubule Anatomy 0.000 description 2
- 230000003287 optical effect Effects 0.000 description 2
- 230000007306 turnover Effects 0.000 description 2
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 1
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 1
- 102000004163 DNA-directed RNA polymerases Human genes 0.000 description 1
- 108090000626 DNA-directed RNA polymerases Proteins 0.000 description 1
- 101710183280 Topoisomerase Proteins 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000006073 displacement reaction Methods 0.000 description 1
- 238000012921 fluorescence analysis Methods 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 238000004904 shortening Methods 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
本实用新型公开了一种单分子操纵横向磁镊装置，包括：显微成像装置、磁镊装置、样品槽和中央监控器，磁镊装置包括磁镊和磁镊控制器，磁镊水平设置在样品槽的侧面，并由磁镊控制器控制；样品槽为一透明的密闭容器，两端有供溶液进出的开口，生物单分子的一端连接在该透明容器内远离磁镊的侧壁上，另一端连接一顺磁性磁球，该磁球通过磁镊控制；显微成像装置设置在样品槽的上部或下部，显微成像装置与样品槽可在水平方向做相对平移；显微成像装置将获取的图像信息传输给中央监控器进行数据处理。本实用新型的有益效果是：可对生物单分子进行研究分析，包括生物分子相关的酶及蛋白等，并可直接给出单分子的长度信息，并可对生物单分子进行旋转操作。
The utility model discloses a single-molecule manipulation transverse magnetic tweezers device, comprising: a microscopic imaging device, a magnetic tweezers device, a sample tank and a central monitor, the magnetic tweezers device includes magnetic tweezers and a magnetic tweezers controller, and the magnetic tweezers are horizontally arranged on The side of the sample tank is controlled by the magnetic tweezers controller; the sample tank is a transparent airtight container with openings at both ends for the solution to enter and exit, and one end of the biological single molecule is connected to the side wall of the transparent container away from the magnetic tweezers. The other end is connected to a paramagnetic magnetic ball, which is controlled by magnetic tweezers; the microscopic imaging device is set on the upper or lower part of the sample tank, and the microscopic imaging device and the sample tank can be relatively translated in the horizontal direction; the microscopic imaging device will The acquired image information is transmitted to the central monitor for data processing. The beneficial effect of the utility model is that the research and analysis of the biological single molecule can be carried out, including the enzyme and protein related to the biological molecule, and the length information of the single molecule can be directly given, and the biological single molecule can be rotated.
Description
Technical field
The utility model relates to a kind of single molecule manipulation magnetic tweezers, belongs to single molecule manipulation and detection range.
Background technology
Monomolecular instrument of many researchs and technology appearred in past 10 years, for example, and light tweezer, magnetic tweezer, atomic force microscope, glass microtubule or the like.The development of small power measuring technique makes the elastic property of studying nucleic acid molecules on single molecules level and the structural change that external force causes become possibility.At present, these technology are applied to research and interactional enzyme of DNA and albumen, for example archaeal dna polymerase, RNA polymerase, topoisomerase etc. more and more.Simultaneously, this technology also can be used for studying the molecular motor of a series of DNA of acting on.
In all these equipment, dna molecular or other big molecule one end are connected in the surface, and the other end connects a force transducer.This force transducer is the bead or the cantilever of micron dimension often.The displacement of bead or cantilever can be used to calculate the stressed size of unimolecule.Different experimental facilitiess provides the different dynamometry scope and the time scale of measurement.In addition, except that stretching molecule, can reach the purpose of control magnetic ball rotation by the rotation of rotating magnetic field direction or control glass microtubule, thereby rotate big molecule.
1992, people such as Smith washed away by flow force and magnetic attraction paramagnetism bead is controlled single biomacromolecule, and studied its character.Though the method can be observed monomolecular fluorescence, extremely complicated to the measurement of single molecular length, and also error is also very big.1996, people's utility models such as Strick vertical magnetic tweezer operating control, measure monomolecular length by optical sensor, but, can't observe unimolecule with fluorescent method because monomolecular prolonging direction is parallel to the optical axis of micro objective.As shown in Figure 1.
The utility model content
Problem at above-mentioned existence, the purpose of this utility model is to provide a kind of monomolecular control transverse magnetic forceps device, the molecule that feasible quilt is handled moves in the focal plane of micro objective, can simplify unimolecule measurement of length problem, and can use fluorescent method simultaneously and observe unimolecule.
For achieving the above object, a kind of monomolecular control transverse magnetic forceps device of the utility model comprises: microscopic imaging device, magnetic tweezers, sample cell and central monitor, described magnetic tweezers comprises magnetic tweezer and magnetic tweezer controller, the magnetic tweezer is positioned horizontally in the side of described sample cell, and by described magnetic tweezer controller control; Described sample cell is a transparent closed container, and there is the opening for the solution turnover at two ends, and an end of single biomolecule is connected on the interior sidewall away from described magnetic tweezer of this transparent vessel, and the other end connects a paramagnetism magnetic ball, and this magnetic ball is by described magnetic tweezer control; Described microscopic imaging device is arranged on the top or the bottom of sample cell, but microscopic imaging device and the relative translation of sample cell along continuous straight runs, to seek biologic single molecular and to obtain the image of biologic single molecular length direction; Described microscopic imaging device is transferred to described central monitor with the image information of obtaining and carries out data processing.
Further, described imaging device comprises light source, object lens and CCD (charge-coupled image sensor), and the described sample cell of light illumination also shows and record, described object lens and the relative translation in the horizontal direction of described sample cell at described central monitor by object lens and CCD.
Further, described magnetic tweezer is that the end is the cylinder-shaped magnetic material of taper, or the two block length strip magnetic materials that separated by the non-magnetic sheet, or U magnet.
Further, described magnetic material is permanent magnet or electromagnet.
The beneficial effects of the utility model are:
(1) can research and analyse biologic single molecular, comprise enzyme that biomolecule is relevant and protein etc.;
(2) can manipulate and measure monomolecular elongation and rotation directly;
(3) can carry out fluorescence analysis to unimolecule.
Description of drawings
Fig. 1 is the prior art synoptic diagram;
Fig. 2 is the utility model monomolecular control transverse magnetic forceps device synoptic diagram;
Fig. 3 is the utility model partial structurtes synoptic diagram (comprising sample cell);
Fig. 4 is (a) and (b) be respectively the structural representation of U type magnetic tweezer and cylindrical magnetic tweezer.
Fig. 5 is the stretching photo of single DNA;
Fig. 6 is the stress strain curve of single DNA;
Fig. 7 is the curve of the length of single DNA with the DNA rotation change.
Embodiment
As shown in Figure 2, microscopic imaging device 1, magnetic tweezers 2, central monitor 3 and sample cell 4, micro-magnetic tweezers 2 comprise magnetic tweezer 5 and magnetic tweezer controller 6, and magnetic tweezer 5 is horizontally set on the side of sample cell 4, and by 6 controls of magnetic tweezer controller.Magnetic tweezer controller 6 can be stepper motor, also can be that other can drive magnetic tweezer 5 in mechanism that X, Y, Z direction move and rotate; As shown in Figure 3, sample cell 4 is a transparent closed container, and there is the opening 15,16 for the solution turnover at two ends.This sample cell 4 is placed on the sample stage 9.The end of single biomacromolecule is connected on the interior sidewall away from magnetic tweezer 5 of this transparent vessel, and the other end connects a paramagnetism magnetic ball, and this magnetic ball is by 5 controls of magnetic tweezer; Microscopic imaging device 1 is arranged on the top or the bottom of sample cell 4, and sample cell 4 and imaging device 1 relative motion in the horizontal direction are to seek biomacromolecule and to obtain the image information of its length direction; Imaging device 1 is transferred to central monitor 3 with the image information of obtaining and carries out data processing, and this central monitor 3 comprises a display 7 and main frame 8, after 8 pairs of images of main frame are handled, is shown by display 7, and can control magnetic tweezer controller 6.Wherein, imaging device 1 comprises light source 10, object lens 11 and CCD12, and light source 11 illumination sample cells 4 also show and records object lens 11 and sample cell 4 relative translation in the horizontal direction by object lens 11 and CCD12 at central monitor 3; Magnetic tweezer 5 be the end be the vertebra shape cylinder-shaped magnetic material (also can be as shown in Figure 4, two block length strip magnetic material or the U magnets that separated by the non-magnetic sheet.The purpose of this design is the rotary freedom of restriction magnetic ball, thereby can rotate the magnetic ball).Magnetic tweezer 5 is designed to the cylinder-shaped magnetic material that the end is the vertebra shape, can stretch to unimolecule, and the shape that is designed to as shown in Figure 4 just can be rotated and stretching experiment unimolecule.Stressed size of magnetic ball and magnetic field gradient and magnet and bead distance dependent.Put on the magnetic ball in order to obtain enough big power, guarantee that magnet front end gradient is enough big, the magnet front end will be made taper, will guarantee that simultaneously magnetic field gradient is similar in the position of magnetic ball and has only a direction, thereby makes biomacromolecule only stressed on single direction.The magnetic ball is stressed to be given by the following formula:
In the formula, m is a magnetic moment,
B is a magnetic field intensity.
But above-mentioned metering system also is not easy and accurately, and in general to be subjected to force measurement be that Brownian movement by the magnetic ball provides to unimolecule, and formula is as follows:
In the formula, k
BBe Boltzmann constant,
T is an absolute temperature,
＜Z〉be monomolecular average length,
＜(δ x)
2It is the variance of magnetic ball x direction.
In our equipment,＜Z〉and＜(δ x)
2Can directly measure by image.
This device can be implemented in and observe monomolecular elongation in the imaging device focal plane of lens, can measure for example variation of DNA length of biomacromolecule more quickly and accurately, simultaneously owing to there are a plurality of unimolecules to be connected to sample cell side edge, can carry out a plurality of monomolecular measurements simultaneously at every turn, improve conventional efficient.Utilize this device, carry out the stress strain curve of dna single molecule, shown in Fig. 5,6, experiment value and theoretical curve (worm-like-chain model) have well and meet.Simultaneously, utilize this device that DNA has been carried out rotary manipulation because the double-spiral structure of DNA, find along with DNA by increase around self axle rotating cycle, the length of DNA is shortening gradually, as shown in Figure 7.
Claims (4)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200620117879 CN2911668Y (en) | 2006-05-30 | 2006-05-30 | Monomolecular controlled traverse magnetic tweezers |
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| CN 200620117879 CN2911668Y (en) | 2006-05-30 | 2006-05-30 | Monomolecular controlled traverse magnetic tweezers |
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| CN2911668Y true CN2911668Y (en) | 2007-06-13 |
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| CN 200620117879 Expired - Lifetime CN2911668Y (en) | 2006-05-30 | 2006-05-30 | Monomolecular controlled traverse magnetic tweezers |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1869650B (en) * | 2006-05-29 | 2010-09-08 | 中国科学院物理研究所 | Transverse Magnetic Tweezers Device for Single Molecule Manipulation |
| CN102749441A (en) * | 2012-07-05 | 2012-10-24 | 中国科学院物理研究所 | Imaging magnetic tweezers device, and system and method for integrating imaging magnetic tweezers device with single-molecule fluorescence technology |
| CN103344565A (en) * | 2013-04-10 | 2013-10-09 | 温州大学 | Temperature-controlled magnetic tweezer device |
| CN105004702A (en) * | 2015-06-18 | 2015-10-28 | 华中科技大学 | Dual-imaging magnetic tweezer system |
| CN108645795A (en) * | 2018-04-28 | 2018-10-12 | 华南理工大学 | A kind of multichannel list albumen magnetic tweezer investigating method and system |
| CN110320186A (en) * | 2019-06-06 | 2019-10-11 | 华南理工大学 | A multi-channel flow chamber and magnetic tweezers composite detection system and method |
| CN112098376A (en) * | 2019-06-17 | 2020-12-18 | 中国科学院物理研究所 | Magnetic tweezers fluorescent device |
-
2006
- 2006-05-30 CN CN 200620117879 patent/CN2911668Y/en not_active Expired - Lifetime
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1869650B (en) * | 2006-05-29 | 2010-09-08 | 中国科学院物理研究所 | Transverse Magnetic Tweezers Device for Single Molecule Manipulation |
| CN102749441A (en) * | 2012-07-05 | 2012-10-24 | 中国科学院物理研究所 | Imaging magnetic tweezers device, and system and method for integrating imaging magnetic tweezers device with single-molecule fluorescence technology |
| CN103344565A (en) * | 2013-04-10 | 2013-10-09 | 温州大学 | Temperature-controlled magnetic tweezer device |
| CN105004702A (en) * | 2015-06-18 | 2015-10-28 | 华中科技大学 | Dual-imaging magnetic tweezer system |
| CN108645795A (en) * | 2018-04-28 | 2018-10-12 | 华南理工大学 | A kind of multichannel list albumen magnetic tweezer investigating method and system |
| CN108645795B (en) * | 2018-04-28 | 2020-01-14 | 华南理工大学 | Multi-channel single-protein magnetic tweezers measurement and control method and system |
| CN110320186A (en) * | 2019-06-06 | 2019-10-11 | 华南理工大学 | A multi-channel flow chamber and magnetic tweezers composite detection system and method |
| CN112098376A (en) * | 2019-06-17 | 2020-12-18 | 中国科学院物理研究所 | Magnetic tweezers fluorescent device |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| AV01 | Patent right actively abandoned |
Granted publication date: 20070613 Effective date of abandoning: 20100908 |
|
| AV01 | Patent right actively abandoned |
Granted publication date: 20070613 Effective date of abandoning: 20100908 |