CN2793720Y - Biological chip scanning device - Google Patents

Abstract

The utility model relates to a biological wafer scanning device, which comprises a light source capable of emitting light; a rotatable grating hologram is arranged at one side of the light source, and the incident light can be diffracted to form diffracted light; arranging a sampling table at one end of the grating type hologram, and placing a sampling sample above the sampling table; and a linear Charge Coupled Device (CCD) for receiving the light reflected from the sample irradiated by the diffracted light. The rotatable grating hologram can make the incident light generate uniform diffraction, so as to obtain more accurate signal-to-noise ratio and ensure the accuracy of light signal. In addition, the light source can be replaced by a linear light source to achieve the effects of greatly increasing the scanning speed and reducing the time consumption.

Description

Biowafer scanning device

technical field

The utility model relates to a biological chip scanning device, in particular to a biological chip scanning device which can obtain a more accurate signal-to-noise ratio to ensure the correctness of light signals.

Background technique

Biotechnology is advancing rapidly, especially the detection of DNA is a very important source of information for human genetics, disease and identification, and plays a key role in the prevention of early tumors and the tracking of drug effectiveness. Therefore, the accuracy and precision of the DNA detection instrument form a necessary condition. Basically, the DNA detection instrument is a biological chip scanner. Its principle is that when the DNA sample is coated on the test piece, in order to detect the difference of the type such as A, G, C, T four kinds of nucleotides, it is necessary to add fluorescent dyes Planted on the test strip first, when the light of the appropriate wavelength is irradiated on the sample, different fluorescence brightness reactions will be produced due to the difference in the DNA type, so as to determine the presence or absence of nucleotides. Since the fluorescence response is weak, the SNR (signal-to-noise ratio) of the fluorescence brightness response detection signal becomes very important.

Please refer to Fig. 1, which is a schematic structural view of a known front-projection reflective biochip scanning device, as shown in the figure: the biochip scanning device 1 includes a laser emitter 11 that can emit laser light; The excitation spectrum filter (Excitation Spectral Filter) 12 of the wavelength accuracy of the excitation light source required by the dyeing agent; one side can make the light transmit, and the other side can reflect the light beam splitter 13; one can transmit the light through the beam splitter 13 Focusing focusing objective lens 14; a sampling platform 15 with the sample 151 to be inspected is placed on the top, and the sampling platform 15 can move freely in the X-Y direction, and the light focused by the focusing objective lens 14 can be used for single-point scanning on the sampling sample 151 Aiming; a fluorescence emission spectrum filter (Fluorescent Emission Spectral Filter) 16 used to filter the fluorescent signal excited by the scanned sample 151; a sensor that can focus the fluorescence signal passing through the fluorescence emission spectrum filter 16 an imaging lens 17 ; and a signal sensing receiver 18 capable of receiving the fluorescent signal focused by the sensor imaging lens 17 . When scanning, the sampling stage 15 can move quickly in the X and Y axis directions, so that all the samples 151 under inspection can be scanned by a single point for analysis. However, although moving the sampling table 15 back and forth for scanning can achieve the function of single-point scanning for analysis, it will cause time-consuming and system vibration problems.

Please refer to FIG. 2, which is a schematic structural diagram of a known back-projection transmission biochip scanning device, as shown in the figure:

The structure of the rear-projection transmissive bio-wafer scanning device 2 is basically the same as that of the above-mentioned front-projection reflective bio-wafer scanning device 1 , except that the beam splitter 13 is removed in terms of structure. The functions and principles of single-point scanning are the same, but they also have time-consuming and system vibration problems.

Please refer to FIG. 3 , which is a schematic structural view of another known front projection reflective biochip scanning device, as shown in the figure: the structure of the front projection reflective biochip scanning device 3 is basically the same as the above-mentioned front projection reflective biochip scanning device. The scanning device 1 is substantially the same, except that a rotating reflector 31 is provided between the reflector 13 and the focusing objective lens 14 and can rotate in the X direction to change the reflection angle of light. When scanning, the rotating mirror 31 can be used to rotate the angle to change the light reflection angle so that the light can be sampled and scanned in the X-axis of the sampling table 15, so that the X-axis can be saved and the scanning can be greatly reduced. Undesirable effects of platform vibration.

Please refer to FIG. 4 , which is a structural schematic diagram of another known front-projection reflective biochip scanning device, as shown in the figure: the structure of the front-projection reflective biochip scanning device 4 is basically the same as that of the above-mentioned front-projection reflective biochip The scanning device 3 is roughly the same, except that the rotating reflective polygon mirror 31 is replaced by a rotating reflective polygon mirror 41 in structure, and its function and principle are the same as that of the above, which can greatly reduce the adverse effects of platform vibration.

Please refer to FIG. 5 , which is a structural schematic view of another known rear-projection transmissive biochip scanning device, as shown in the figure: the structure of the rear-projection transmissive biochip scanning device 5 is basically the same as that of the above-mentioned front-projection reflective biochip The scanning device 4 is substantially the same, except that the structure is changed to a rear-projection transmission type. In addition, the signal sensing receiver 18 can also use a linear charge-coupled device (CCD).

However, as shown in Fig. 3, Fig. 4 and Fig. 5, although the use of the rotating reflector 31 or the rotating reflective polygon mirror 41 can save the moving and scanning in the X-axis, and can greatly reduce the adverse effects of the platform vibration, but this method is very easy to be caused by The swing or rotation of the reflective mirror produces a deflection of the rotation axis, which causes the illumination light source to be unable to focus on the sample, and due to the different rotation angles, the beam of the illumination light at the edge of the test piece will be deformed into an ellipse, resulting in insufficient and uneven illumination , causing the signal to generate an error.

Utility model content

The main purpose of the utility model is to provide a biological chip scanning device, which can obtain a more accurate signal-to-noise ratio to ensure the correctness of the light signal.

Another object of the present invention is to provide a biochip scanning device, which can greatly increase the scanning speed.

Another object of the present invention is to provide a biochip scanning device, which can achieve the effect of reducing time consumption.

In order to achieve the above object, the biological wafer scanning device provided by the utility model includes:

a light source capable of emitting light;

A sampling platform, which is located on one side of the light source, and a sampling sample can be placed on the sampling platform, so that the light of the light source is irradiated on the sampling sample on the sampling platform; and

A charge-coupled device is above the sampling table, and the charge-coupled device can receive the light reflected by the sampling sample.

The light emitted by the light source can be linear light.

One side of the light source is provided with a rotating grating-type hologram, so that the light from the light source passes through the rotating grating-type hologram, and the incident light is diffracted to form diffracted light.

The type of the light source can be one of laser light, incandescent light and gas excitation light source.

Wherein the sampling platform can move freely in the X-Y direction.

Wherein the light of the light source passes through the rotating grating type hologram, and an excitation spectrum filter may be arranged between the light source and the grating type hologram.

Wherein the light from the light source passes through the rotating grating hologram, and a focusing objective lens may be provided between the grating hologram and the sampling platform.

Wherein the light of the light source passes through the rotating grating hologram, and a beam splitter can be arranged between the grating hologram and the sampling stage.

The light from the light source passes through the rotating grating-type hologram to form diffracted light, and the diffracted light is irradiated on the sampling sample, and the light reflected by the sampling sample can pass through a fluorescence emission spectrum filter and then be transmitted to the linear charge coupling device.

The light from the light source passes through the rotating grating-type hologram to form diffracted light, and the light reflected by the sampling sample after being irradiated by the diffracted light can be transmitted to the linear charge-coupled device through a sensor imaging lens.

Wherein the light emitted by the light source is linear light, an excitation spectrum filter can be arranged between the light source and the sampling platform.

Wherein the light emitted by the light source is linear light, a light splitter can be arranged between the light source and the sampling platform.

Wherein the light emitted by the light source is a linear light, a focusing objective lens can be arranged between the light source and the sampling platform.

Wherein the light emitted by the light source is linear light, the linear light irradiates the sampling sample, and the light reflected by the sampling sample can pass through a fluorescent emission spectrum filter and then be transmitted to the linear charge-coupled device.

Wherein the light emitted by the light source is a linear light, and the light reflected by the sampling sample after being irradiated by the linear light can pass through a sensor imaging lens and then be transmitted to the linear charge-coupled device.

Description of drawings

FIG. 1 is a schematic structural diagram of a known front-projection reflective biological wafer scanning device.

FIG. 2 is a schematic structural diagram of a conventional rear-projection transmission type biological wafer scanning device.

FIG. 3 is a schematic structural diagram of another known front-projection reflective biological wafer scanning device.

FIG. 4 is a structural schematic diagram of yet another known forward projection reflective biological wafer scanning device.

FIG. 5 is a schematic structural diagram of another conventional rear-projection transmission biological wafer scanning device.

Fig. 6 is a diagram of an embodiment of the utility model.

Fig. 7 is another embodiment figure of the utility model.

Figure 8 is a comparison diagram of the deflection of the mirror surface and the hologram.

Fig. 9 is another embodiment diagram of the present utility model.

Fig. 10 is another embodiment diagram of the utility model.

Specific implementation method

In order to further understand the purpose, features and effects of the present utility model, the utility model is described in detail by the following specific embodiments in conjunction with the accompanying drawings, as follows:

Please refer to Fig. 6, which is a diagram of an embodiment of the present utility model, as shown in the figure:

The front projection reflective biochip scanning device 6 includes a light source 61 that can emit light, and the light source 61 can be a single point light source, and its type can be one of laser, incandescent light and gas excitation light source; One side of the light source 61 is provided with a rotatable grating type hologram 63, which can make the incident light diffract and form a diffracted light; A sampling sample 651 is placed, and the sampling platform 65 can move freely in the X-Y direction, so that the sampling sample 651 on it can be scanned at a single point; The light reflected from sample 651 is sampled. In addition, an excitation spectrum filter 62 can be added between the light source 61 and the grating-type hologram 63 to filter the accuracy of the light source at the excitation wavelength required by the fluorescent dye; between the grating-type hologram 63 and the sampling A focusing objective lens 64 and a beam splitter 69 can be added between the tables 65. The focusing objective lens 64 can focus the light transmitted through the grating type hologram 63. One side of the beam splitter 69 can transmit the light, and the other side can sample the light. The fluorescent signal generated by the sample 651 is reflected by the irradiation; the fluorescent signal generated by the sample 651 by the irradiation is reflected by the spectrometer 69, and can pass through a fluorescence emission spectrum filter 66 to filter the sample 651 to be excited and reflected after scanning Fluorescence signal, and a sensor imaging lens 67 can focus the fluorescence signal passing through the fluorescence emission spectral filter 66, and then transmit it to a linear charge-coupled device (CCD) 68. When scanning, the rotating grating-type hologram 63 diffracts the incident laser beam evenly onto the sampling sample 651, and after being corrected by the focusing objective lens 64, the incident laser light can be irradiated to the sampling stage completely uniformly 65, so that a more accurate signal-to-noise ratio can be obtained to ensure the correctness of the signal. The advantage of using this rotating grating type hologram 63 is that the problem caused by the deflection of the rotation axis can be largely eliminated, and the prerequisite is that the incident angle of the laser light source is preferably controlled at about 1° to 30° of the Bragg regime. The angle of diffraction will be equivalent to the angle of incidence, and the small amount of rotation axis deflection can be ignored, so the angle deviation produced is about one thousandth of that when using polygon mirrors or rotating mirrors, and can greatly improve the grating type hologram The mass production requirements of sheet 63.

Please refer to Fig. 7, which is another embodiment figure of the present utility model, as shown in the figure:

The structure of the rear-projection transmissive bio-wafer scanning device 7 is basically the same as the above-mentioned front-projection reflective bio-wafer scanning device 6 , only the structure is changed to the rear-projection transmissive type, and the beam splitter 69 is removed. Its function and principle are the same as that of the above, which can obtain a more accurate signal-to-noise ratio and ensure the correctness of the signal.

Please refer to Figure 8, which is a comparison diagram of the deflection of the mirror surface and the hologram, as shown in the figure:

The left figure in the figure is the relationship between the deflection angle of the rotation axis of a general polygon mirror and the angle deviation error after reflection, and the right figure in the figure is the relationship between the deviation and the diffraction angle when using a hologram, and the vertical axis on the left is The measure is 1000 times the measure on the right vertical axis. Therefore, it can be clearly seen that the angle deviation produced by using a hologram is about one-thousandth that of using a polygon mirror or a rotating mirror.

Please refer to Fig. 9, which is another embodiment diagram of the present utility model, as shown in the figure:

The front-projection reflective biochip scanning device 8 includes a line light source 81 that can emit light, and the type of the line light source 81 can be one of laser light, incandescent light and gas excitation light source; One side is provided with a spectroscopic sheet 83 that can transmit light and reflect light on the other side; a sampling platform 85 is provided at one end of the spectroscopic sheet 83, and a sampling sample 851 is placed above the sampling platform 85, and the sampling platform 85X- Y can move freely in the X-Y direction, so that the sampling sample 851 on it can be scanned at a single point; and a line-type charge-coupled device (CCD) 88 can receive light reflected from the line light source 81 to the sampling sample 851. In addition, an excitation spectrum filter 82 can be added between the line light source 81 and the light splitter 83 to filter the accuracy of the light source at the excitation wavelength required by the fluorescent dye; There is a focusing objective lens 84, which can focus the light passing through the beam splitter 83 and irradiate it onto the sampling sample 851; the fluorescent signal generated by the sampling sample 851 penetrates into a fluorescence emission spectrum filter 86 for filtering After the sample 851 is scanned, the excited and reflected fluorescent signal, together with a sensor imaging lens 87 , can focus the fluorescent signal passing through the fluorescent emission spectrum filter 86 , and then transmit it to a linear charge-coupled device (CCD) 88 .

Please refer to Fig. 10, which is another embodiment diagram of the present utility model, as shown in the figure:

The structure of the rear-projection transmissive bio-wafer scanning device 9 is basically the same as that of the above-mentioned front-projection reflective bio-wafer scanning device 8 , only the structure is changed to the rear-projection transmissive type, and the beam splitter 83 is removed.

As shown in Figures 9 and 10, when scanning, the scanned sample is irradiated with a light source in the form of a linear light source, so that the number of scanning points increases at one time. When using a linear charge-coupled device, it can be simultaneously Receive signal exposure sampling, greatly improve scanning speed, increase efficiency and reduce time consumption.

The above descriptions are only preferred embodiments of the present utility model, and are not intended to limit the patent scope of the present utility model; all other equivalent changes or modifications completed without departing from the spirit disclosed by the present utility model shall be Included within the scope of the patent application.

Claims (15)

1. A biochip scanning device, characterized in that it comprises: a light source emitting light; a sampling platform, located on one side of the light source, and a sampling sample is placed on the sampling platform, and the light of the light source irradiates the sampling sample on the sampling platform; and A charge-coupled device is above the sampling table, and the charge-coupled device receives the light reflected by the sampling sample. 2. The biological wafer scanning device as claimed in claim 1, wherein the light emitted by the light source is a linear light. 3. The biological wafer scanning device according to claim 1, wherein a rotating grating-type hologram is arranged on one side of the light source, and the light of the light source passes through the rotating grating-type hologram, so that The incident light is diffracted to form diffracted light. 4. The biological wafer scanning device as claimed in claim 1, wherein the type of the light source is one of laser light, incandescent light and gas excitation light source. 5. The biological wafer scanning device as claimed in claim 1, wherein the sampling table is free to move in the X-Y direction. 6. The biological wafer scanning device according to claim 3, wherein the light from the light source passes through the rotating grating hologram, and an excitation spectrum filter is arranged between the light source and the grating hologram . 7. The biological wafer scanning device according to claim 3, wherein the light from the light source passes through the rotating grating hologram, and a focusing objective lens is arranged between the grating hologram and the sampling table . 8. The biowafer scanning device as claimed in claim 3, wherein the light from the light source passes through the rotating grating hologram, and a beam splitter is arranged between the grating hologram and the sampling stage . 9. The biological wafer scanning device according to claim 3, wherein the light from the light source passes through the rotating grating hologram to form diffracted light, and the diffracted light is irradiated on the sampling sample, and the sampling sample The reflected light is transmitted to the linear charge-coupled device through a fluorescence emission spectrum filter. 10. The biological wafer scanning device according to claim 3, wherein the light from the light source passes through the rotating grating-type hologram to form diffracted light, and the light reflected by the sampling sample passes through the diffracted light. A sensor imaging lens is then sent to the linear charge-coupled device. 11. The biochip scanning device according to claim 2, wherein the light emitted by the light source is a linear light, and an excitation spectrum filter is arranged between the light source and the sampling platform. 12. The biological wafer scanning device as claimed in claim 2, wherein the light emitted by the light source is a linear light, and a beam splitter is provided between the light source and the sampling table. 13. The biological wafer scanning device as claimed in claim 2, wherein the light emitted by the light source is a linear light, and a focusing objective lens is provided between the light source and the sampling table. 14. The biochip scanning device as claimed in claim 2, wherein the light emitted by the light source is a linear light, and the linear light irradiates the sampling sample, and the light reflected by the sampling sample passes through a The fluorescence emission spectrum filter is sent to the linear charge-coupled device. 15. The biological wafer scanning device as claimed in claim 2, wherein the light emitted by the light source is a linear light, and the light reflected by the sampling sample after being irradiated by the linear light passes through a sensor imaging lens and then sent to the linear charge-coupled device.

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