Fanless constant temperature incubator
Technical Field
The utility model relates to the technical field of microorganism constant temperature incubators, in particular to a fan-free constant temperature incubator.
Background
The CO2 constant temperature incubator is a device for culturing cells and tissues by forming a growth environment similar to cells and tissues in a living body in the incubator, wherein the incubator requires stable temperature, stable CO2 concentration and uniformity and high relative saturation humidity. Is widely applied to the research and production of the scientific fields of microorganisms, medicine, pharmacy, environmental protection, food, livestock and the like.
The CO2 incubator controls the concentration of CO2 by means of a CO2 concentration sensor. The sensor transmits the detection result to control elements such as a control circuit, an electromagnetic valve and the like to control the opening and closing of the CO2 gas until the concentration is stable and reaches a set value. Humidity control is to directly place a humidifying disc inside the box body, and generate moisture through evaporation of water in the disc. There are two general heating modes of incubator on the market, water jacket type heating and air jacket type heating, the water jacket type CO2 constant temperature incubator is heated by heating wire water, and then the temperature change is detected by the temperature sensor of the incubator, so that the temperature in the incubator is constant at the set temperature. The air jacket type CO2 constant temperature incubator directly heats the inner incubator body through the heater distributed in the incubator body, and then the air in the incubator body is uniformly stirred through the fan, so that the temperature and the uniformity of CO2 in the incubator body are ensured.
Although the water jacket type heat preservation effect is better, water needs to be added regularly, the water jacket type heat preservation device is clean and disinfected, if the operation is improper, cell pollution is easy to cause, a high-temperature sterilization system is not arranged, and the pollution risk is higher. The air-jacketed incubator has the advantages of quick heating, quicker temperature recovery than water jacket type incubator, low cost, convenient maintenance and the like.
Most incubators currently adopt an air-jacketed heating mode, but fans are arranged in the air-jacketed incubators for air circulation, and turbulence and vibration caused by the fans can cause uneven cell generation. The box is all integrative welding, and not only processing cost is high, and this kind of structure heat preservation cotton fills into the box after all tailorring, will fill the gap, leads to the temperature uniformity not ideal so. And the liner partition frame is formed by stamping a sheet metal part, is not easy to detach, and is inconvenient to clean and sterilize.
Disclosure of utility model
The utility model aims to provide a fan-free constant-temperature incubator, which aims to overcome the defects of the prior art and solve the problems that the temperature uniformity is not ideal due to filling gaps in heat-insulating cotton, the cell generation is uneven due to vibration of a liner fan, and the liner partition frame is formed by stamping a sheet metal part, so that the liner partition frame is not easy to detach and is inconvenient to clean and sterilize.
The utility model provides a fanless constant temperature incubator which comprises a box body, an inner container, a test box, an inner door and an outer door, wherein heating wires are arranged outside each inner container, the box body is formed by splicing a plurality of panels in a buckle type connection mode, an integrated heat insulation material is filled between the box body and the inner container, the test box is arranged outside the inner container, a CO2 sensor, an axial flow fan and a humidity probe are arranged inside the test box, an air port of the test box is connected with an air port of the inner container through silicon rubber, and box gas is extracted through the axial flow fan for detection.
As a preferable technical scheme of the application, the partition plate frame is fixed on the side wall of the inner container through nuts, and the partition plate frame is formed by welding round steel.
As a preferable technical scheme of the application, the inner container is manufactured by adopting mirror surface stainless steel argon arc welding to form an integrated structure, and four corners of the inner container are semicircular.
As a preferable technical scheme of the application, an ultraviolet sterilizing lamp is arranged in the test box.
As a preferable technical scheme of the application, the liner bottom plate is provided with an independently controlled condensing device.
As a preferable technical scheme of the application, the bottom limit design of the partition plate frame is used for placing the humidifying disc, and meanwhile, the bottom of the inner container is provided with a heating rod.
As a preferable technical scheme of the application, the heating wires arranged outside the inner container are controlled by using independent heating loops.
As a preferable technical scheme of the application, the heating wire is distributed in the outer door, and the outer door is filled with the heat insulation material.
As a preferable technical scheme of the application, the inner door adopts a toughened glass door.
The fan-free constant temperature incubator provided by the utility model has the advantages that the incubator body is in buckle type connection, the disassembly is convenient, the filling of heat insulation materials is realized, the fan-free design is realized in the inner container, the turbulence and vibration are reduced, the uniformity of cell growth is ensured, the external test box is used for carrying out CO2 control through the CO2 concentration sensor, the ultraviolet sterilization lamp is used for regularly carrying out ultraviolet sterilization on the incubator body, so that pollution during cell culture is effectively prevented, the detachable structure of the baffle frame is convenient for cleaning and sterilization, and the design of the condensation point at the bottom is used for avoiding the formation and drip of condensed water at other parts in the incubator body and polluting test samples.
In addition to the objects, features and advantages described above, the present application has other objects, features and advantages. The present application will be described in further detail with reference to the drawings.
Drawings
The accompanying drawings, which are included to provide a further understanding of the application and are incorporated in and constitute a part of this specification, illustrate embodiments of the application and together with the description serve to explain the application. In the drawings:
FIG. 1 is a schematic diagram of the overall structure of a fanless incubator of the present utility model;
FIG. 2 is a schematic diagram showing the installation of a buckle structure in a fanless constant temperature incubator of the present utility model;
FIG. 3 is a schematic view of the structure of the inside of a test box in a fanless incubator of the present utility model;
FIG. 4 is a schematic view of the structure of the partition frame of the fanless incubator of the present utility model;
FIG. 5 is a schematic diagram of the operation of the fanless incubator of the present utility model;
The reference numerals indicate that 1, a box body, 2, an inner container, 3, a test box, 4, a partition plate frame, 5, an inner door, 6, an outer door, 7, a condensing device, 8, a heat insulation material, 9, a heating wire, 10, a CO2 sensor, 11, an axial flow fan, 12, a humidity probe, 13, an ultraviolet sterilizing lamp, 14, a nut, 15 and a humidifying disc.
Detailed Description
It should be noted that, without conflict, the embodiments of the present application and features of the embodiments may be combined with each other. The application will be described in detail below with reference to the drawings in connection with embodiments.
As shown in figures 1-5, the fanless constant temperature incubator of the utility model comprises a box body 1, an inner container 2, a test box 3, a partition plate frame 4, an inner door 5, an outer door 6 and a bottom condensing device 7.
Specifically, as shown in fig. 2, the material of the box body 1 is carbon steel plastic spraying, the thermal insulation material 8 fills the box body, the box body 1 is connected in a buckle mode, and the thermal insulation material is convenient to fill integrally by adopting a buckle structure, so that the processing cost is greatly reduced. The box is detachable buckle structure, but not traditional whole welded structure, and this kind of structure insulation material can wholly fill, wraps up the inner bag around, need not to tailor, plays better heat preservation effect.
The inner container 2 is manufactured by adopting mirror surface stainless steel argon arc welding, an integrated structure is formed, four corners are semicircular and easy to clean, heating wires 9 are arranged on each surface of the inner container 2 to independently control a heating loop, the box body is directly heated, heat transfer is carried out instead of a fan design, the consistency of temperature is ensured, the fan design is not arranged inside, turbulence and vibration are reduced, and the cells cultured on each layer are ensured to be identical.
As shown in fig. 1 and 3, the test box 3 is arranged outside the inner container 2, and the air port of the test box 3 is connected with the air port of the inner container 2 through silicon rubber, the CO2 sensor 10 is installed inside the test box 3, the axial flow fan 11 extracts CO2 gas in the inner container to the test box 3, the concentration of CO2 in the box body is detected through the CO2 sensor, the humidity of the box body is detected through the humidity probe 12, and the ultraviolet sterilizing lamp 13 in the test box regularly performs ultraviolet sterilization on the gas in the box body, so that pollution during cell culture is effectively prevented. The test box 3 is arranged outside the inner container, thereby being convenient for cleaning the inner container and increasing the internal use area.
As shown in figure 4, the baffle frame 4 is welded by round steel and fixed on the side wall of the inner container 2 by screwing nuts 14 by hand, the inner door 5 is a toughened glass door, so that cells can be observed conveniently and box gas can be sealed better, the baffle frame 4 is welded by round steel and screwed by hand and fixed, disassembly and installation are convenient, the bottom limit design of the baffle frame 4 can be realized, a humidifying disc 15 can be placed, and the whole detachable structure is convenient for cleaning and disinfection of the inside of the box.
Meanwhile, a heating rod is arranged at the bottom of the inner container, the generated relative humidity can reach about 95% through the moisture generated by the evaporation of the humidifying disc, and then the humidity sensor in the test box is used for measuring, so that the actual numerical value is displayed on the liquid crystal screen.
The heating wire 9 is distributed in the outer door 6, the heat insulation material is filled, the generation of condensed water is effectively prevented, the independently controlled condensing device 7 is arranged on the bottom plate of the inner container 2, the temperature is the lowest temperature point of the whole box body, and the condensed water is prevented from forming at other parts in the box body, dripping and polluting a culture sample.
The working principle and working process of the fanless constant temperature incubator of the present utility model are briefly described below.
As shown in fig. 5, the fanless constant temperature incubator directly heats each surface of the incubator body by using a heating wire, does not need a fan to uniformly mix gas for heat transfer, and detects temperature change by a temperature sensor on the incubator body so as to ensure that the temperature in the incubator body is constant at a set temperature. The axial flow fan in the test box sucks CO2 gas in the inner container into the test box through the air suction port communicated with the inner container, the CO2 concentration is detected by the CO2 sensor, the detection result is transmitted to control elements such as a control circuit and an electromagnetic valve, if the detection result shows that the concentration of CO2 in the box body is lower, the electromagnetic valve is opened, CO2 enters the box body until the concentration of CO2 reaches a set value, at the moment, the electromagnetic valve is closed, and the CO2 in the box body is cut off.
The humidity is the humidity generated by the evaporation of the humidifying disc, the generated relative humidity can reach about 95%, and then the actual numerical value is displayed on the liquid crystal screen through a humidity sensor in the test box. The ultraviolet sterilizing lamp can regularly sterilize the gas in the test box through program setting, and the sucked gas is discharged to the box body through the gas outlet after detection and sterilization are completed, so that a closed loop is formed.
The above description is only of the preferred embodiments of the present application and is not intended to limit the present application, but various modifications and variations can be made to the present application by those skilled in the art. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application should be included in the protection scope of the present application.