CN211035866U - A plunger-type nucleic acid detection integrated cartridge - Google Patents
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Abstract
本实用新型公开了一种柱塞式核酸检测一体化卡盒。所述一体化卡盒包括反应室组、试剂室组、均液室组、检测室组和活动柱塞，反应室组与试剂室组、废液室和均液室组通过管道连接，均液室组与检测室组通过管道对应连接，所述反应室组与试剂室组和均液室组间均设有阀门。本实用新型所述柱塞式核酸检测一体化卡盒是一个全封闭体系，能够完成病菌检测的整个过程，包括核酸提取、扩增/杂交和检测，能够保证被测样品不会混淆，同时不会造成气溶胶污染，减小检测人员被病菌感染的机率。本实用新型所述柱塞式核酸检测一体化卡盒可通过外部动力控制实现自动化检测，具有很强的实用性。
The utility model discloses a plunger-type nucleic acid detection integrated cartridge. The integrated cartridge includes a reaction chamber group, a reagent chamber group, a liquid homogenization chamber group, a detection chamber group and a movable plunger. The chamber group and the detection chamber group are correspondingly connected through pipelines, and valves are provided between the reaction chamber group, the reagent chamber group and the liquid homogenization chamber group. The plunger-type nucleic acid detection integrated cartridge of the utility model is a fully enclosed system, which can complete the entire process of bacteria detection, including nucleic acid extraction, amplification/hybridization and detection, and can ensure that the tested samples will not be confused, and at the same time, no It will cause aerosol contamination and reduce the probability of detection personnel being infected by germs. The plunger-type nucleic acid detection integrated cartridge of the utility model can realize automatic detection through external power control, and has strong practicability.
Description
技术领域technical field
本实用新型涉及医疗器械技术领域，更具体地，涉及一种柱塞式核酸检测一体化卡盒。The utility model relates to the technical field of medical devices, in particular to a plunger-type nucleic acid detection integrated cartridge.
背景技术Background technique
目前，致病菌检测主要方法有培养法、免疫分析法和核酸法。At present, the main methods of pathogenic bacteria detection are culture method, immunoassay method and nucleic acid method.
培养法是将不同的细菌培养在不同的培养基，大多非可培养状态菌无法通过培养被检测；不同菌体可能产生相同的特异性物质，产生假阳性菌落。免疫分析法是利用抗原-抗体反应的特异性对目标物质进行检测的方法，包括酶免疫技术、荧光免疫技术和免疫磁分离技术等。这两种方法的操作过程时间长，对外界环境要求高，检测结果可靠性不强。The culture method is to cultivate different bacteria in different media, most of the non-culturable state bacteria cannot be detected by culture; different bacteria may produce the same specific substances, resulting in false positive colonies. Immunoassay is a method that uses the specificity of antigen-antibody reaction to detect target substances, including enzyme immunoassay, fluorescence immunoassay, and immunomagnetic separation technology. The operation time of these two methods is long, the requirements for the external environment are high, and the reliability of the detection results is not strong.
核酸法是应用分子生物学方法，检测致病菌遗传物质结构或表达水平的变化，继而作出诊断的技术。核酸的检测过程包括样本前处理与纯化、核酸扩增/杂交与检测等。在检测过程中，传统的检测方法人为操作过程复杂，自动化程度不高，样本检测时容易出现交叉污染、结果出现假阳性等一系列问题，并且检测实验的开放环境容易增加工作人员感染病菌的机率。Nucleic acid method is a technology that uses molecular biology methods to detect changes in the structure or expression level of genetic material of pathogenic bacteria, and then make a diagnosis. The nucleic acid detection process includes sample pretreatment and purification, nucleic acid amplification/hybridization and detection, etc. In the detection process, the traditional detection method has complicated manual operation process and low degree of automation. A series of problems such as cross-contamination and false positive results are prone to occur during sample detection, and the open environment of the detection experiment is easy to increase the probability of staff being infected with bacteria. .
发明内容SUMMARY OF THE INVENTION
本实用新型要解决的技术问题是针对现有技术中检测效率低、准确度不高、易用性不强的不足，提供一种柱塞式核酸检测一体化卡盒。The technical problem to be solved by the utility model is to provide a plunger-type nucleic acid detection integrated cartridge, aiming at the shortcomings of low detection efficiency, low accuracy and low ease of use in the prior art.
本实用新型的目的通过以下技术方案予以实现：The purpose of the present utility model is achieved through the following technical solutions:
一种柱塞式核酸检测一体化卡盒，包括反应室组、试剂室组、检测室组和活动柱塞，所述反应室组、试剂室组、检测室组为卡盒内置的中空腔体，腔体的开口在卡盒表面，所述活动柱塞与反应室组、试剂室组和检测室组的腔体相匹配，活动柱塞可借助外部动力在腔体内上拉或下推；所述反应室组与试剂室组通过管道连接，反应室组与检测室组通过管道连接；所述反应室与试剂室组、反应室组和检测室组间设有阀门。A plunger-type nucleic acid detection integrated cartridge, comprising a reaction chamber group, a reagent chamber group, a detection chamber group and a movable plunger, wherein the reaction chamber group, the reagent chamber group and the detection chamber group are hollow cavities built in the cartridge , the opening of the cavity is on the surface of the cartridge, the movable plunger matches the cavity of the reaction chamber group, the reagent chamber group and the detection chamber group, and the movable plunger can be pulled up or pushed down in the cavity with the help of external power; The reaction chamber group and the reagent chamber group are connected by pipes, and the reaction chamber group and the detection chamber group are connected by pipes; valves are arranged between the reaction chambers and the reagent chamber group, the reaction chamber group and the detection chamber group.
进一步地，反应室组和检测室组之间还设有均液室组，均液室底部有管道与检测室对应连接，连接管道设有流体双向阀门或流体单向阀门。优选地，所述均液室组内的均液室位于同一平面，均液室之间由相同高度的管道连接，有效的保证检测室内的溶液均分，减小检测误差。进一步优选地，所述均液室之间设有阀门。Further, a liquid homogenization chamber group is also arranged between the reaction chamber group and the detection chamber group, the bottom of the liquid equalization chamber is connected with the detection chamber correspondingly, and the connecting pipe is provided with a fluid two-way valve or a fluid one-way valve. Preferably, the homogenizing chambers in the homogenizing chamber group are located on the same plane, and the homogenizing chambers are connected by pipes of the same height, which effectively ensures the equal distribution of the solution in the detection chamber and reduces the detection error. Further preferably, a valve is provided between the liquid homogenization chambers.
进一步地，所述反应室组至少包括1个反应室，所述试剂室组至少包括1个试剂室，所述均液室组至少包括2个均液室，所述检测组至少包括2个检测室。反应室组可满足核酸提取不同物质反应分开提纯，均液室组和检测室组一一对应连接。优选地，根据试验检测要求，3组检测可定性分析，5组检测可定量或多重分析。Further, the reaction chamber group includes at least one reaction chamber, the reagent chamber group includes at least one reagent chamber, the homogenizing chamber group includes at least two equalizing chambers, and the detection group includes at least two detection chambers. room. The reaction chamber group can satisfy the separation and purification of different substances in nucleic acid extraction, and the homogenization chamber group and the detection chamber group are connected in one-to-one correspondence. Preferably, according to the test requirements, 3 groups of tests can be qualitatively analyzed, and 5 groups of tests can be quantitatively or multiplexed.
进一步地，所述反应室组底部设有磁铁和加热器；所述检测室组底部设有变温器。所述磁铁可将磁性物质吸附在反应室底部，可以与目标物质吸附或反应，也可以防止磁性物质流失或对检测造成影响。加热器和变温器可对反应和检测的温度进行调整。Further, the bottom of the reaction chamber group is provided with a magnet and a heater; the bottom of the detection chamber group is provided with a temperature changer. The magnet can adsorb the magnetic substance on the bottom of the reaction chamber, can adsorb or react with the target substance, and can also prevent the loss of the magnetic substance or affect the detection. Heaters and thermostats allow adjustment of reaction and detection temperatures.
进一步地，所述连接反应室的管道连接处设有流体双向阀门，试剂室组管道连接口设有流体双向阀门，试剂室内的试剂压入反应室反应，反应后可压会原溶剂储存室。Further, a fluid bidirectional valve is provided at the pipe connection connecting the reaction chamber, and a fluid bidirectional valve is provided at the pipe connection port of the reagent chamber group. The reagent in the reagent chamber is pressed into the reaction chamber for reaction, and can be pressed into the original solvent storage chamber after reaction.
进一步地，所述柱塞式核酸检测一体化卡盒还包括废液室，所述废液室与反应室组通过管道连接，所述废液室与反应室组之间设有流体单向阀门。溶剂室内溶剂单向进入反应室，反应完后废液直接排进废液室，可保证原溶剂室内的纯度，可多次使用。Further, the plunger-type nucleic acid detection integrated cartridge further includes a waste liquid chamber, the waste liquid chamber and the reaction chamber group are connected by pipes, and a fluid one-way valve is arranged between the waste liquid chamber and the reaction chamber group. . The solvent in the solvent chamber enters the reaction chamber in one direction, and the waste liquid is directly discharged into the waste liquid chamber after the reaction, which can ensure the purity of the original solvent chamber and can be used multiple times.
进一步地，所述外部动力由电机、气泵、液泵的一种或多种方式提供。可通过微处理器对外部动力的控制，实现核酸自动化检测，解决人员因检测病菌而感染的问题。Further, the external power is provided by one or more ways of a motor, an air pump, and a liquid pump. Through the control of the external power by the microprocessor, the automatic detection of nucleic acid can be realized, and the problem of personnel infection caused by the detection of germs can be solved.
进一步地，所述卡盒还设有检测孔，所述检测孔可以对检测室内的溶剂采用对射、散射、反射的一种或多种检测。优选地，所述检测方法为荧光检测、紫外线检测、浊度检测的一种或多种。Further, the cartridge is further provided with a detection hole, and the detection hole can use one or more detections of radiation, scattering and reflection for the solvent in the detection chamber. Preferably, the detection method is one or more of fluorescence detection, ultraviolet detection, and turbidity detection.
进一步地，所述柱塞式检测一体化卡盒可用于分子的纯化和检测一种或多种。优选地，所述柱塞式检测一体化卡盒可用于核酸的提纯、扩增、杂交和检测的一种或多种，也可用于蛋白质纯化和检测的一种或多种。Further, the plunger-type detection integrated cartridge can be used for one or more of the purification and detection of molecules. Preferably, the plunger-type detection integrated cartridge can be used for one or more of nucleic acid purification, amplification, hybridization and detection, and can also be used for one or more of protein purification and detection.
根据上述柱塞式核酸检测一体化卡盒，提供一种高效安全的检测步骤，包括：According to the above-mentioned plunger-type nucleic acid detection integrated cartridge, an efficient and safe detection step is provided, including:
S1.将所需试剂、磁性物质和检测标记物等液体或固体物质分别事先封存进试剂室组和检测室组，活动柱塞密封，再将被检测物放入反应室中，塞上活动柱塞；S1. Store liquid or solid substances such as required reagents, magnetic substances and detection markers in the reagent chamber group and detection chamber group respectively in advance, seal the movable plunger, and then put the detected object into the reaction chamber and plug the movable column plug;
S2.将试剂组中的反应试剂和磁性物质分别通过活动柱塞压入反应室中与被检测物，加热、混匀，通过反应室内活动柱塞和溶剂室的活动柱塞的上拉与下推实现溶液的混匀。S2. Press the reaction reagents and magnetic substances in the reagent group into the reaction chamber through the movable plunger and the detected object respectively, heat and mix, and then pull up and down through the movable plunger in the reaction chamber and the movable plunger in the solvent chamber. Push to mix the solution.
S3.反应完后，通过反应室底部的磁性物质将被检测物吸附在反应室，将反应试剂通过活动柱塞压入原溶剂存放室或废液室。S3. After the reaction is completed, the detected object is adsorbed in the reaction chamber through the magnetic substance at the bottom of the reaction chamber, and the reaction reagent is pressed into the original solvent storage chamber or the waste liquid chamber through the movable plunger.
S4.重复步骤S2~3将各溶剂压入反应室操作，对核酸进行提纯、扩增、杂交的一种或多种，将被检测物与溶剂混合均匀后，将混合溶液压入均液室组均匀分液。S4. Repeat steps S2-3 to press each solvent into the reaction chamber to perform one or more of nucleic acid purification, amplification, and hybridization. After mixing the detected substance and the solvent evenly, press the mixed solution into the homogenizing chamber. The components are evenly dispensed.
S5.将分液室中的混合液压入与之对应的检测室组中，通过检测孔对混合液体进行多重定量和/或定性检测。S5. Put the mixed liquid in the liquid separation chamber into the corresponding detection chamber group, and perform multiple quantitative and/or qualitative detection on the mixed liquid through the detection holes.
与现有技术相比，有益效果是：Compared with the prior art, the beneficial effects are:
本实用新型创造性的将密封卡盒内完成核酸检测的核酸提取、扩增/杂交和检测。本实用新型所述柱塞式核酸检测一体化卡盒是一个全封闭体系，卡盒内试剂事先封存，保证被测样品不会混淆，同时不会造成气溶胶污染，减小检测人员被病菌核酸感染的几率。手动将被测样品加入卡盒内反应室，可通过外部动力对移动柱塞的升降控制溶剂与核酸的反应，对病菌核酸进行提取、扩增/杂交、均液定量，再通过与检测室对应的检测孔进行多个指标检测。The utility model creatively completes nucleic acid extraction, amplification/hybridization and detection of nucleic acid detection in a sealed cartridge. The plunger-type nucleic acid detection integrated cartridge of the utility model is a fully enclosed system, and the reagents in the cartridge are sealed in advance to ensure that the tested samples will not be mixed up, and at the same time, aerosol pollution will not be caused, and the detection personnel will be less affected by pathogenic nucleic acids. chance of infection. Manually add the sample to be tested into the reaction chamber in the cartridge, and the reaction between the solvent and nucleic acid can be controlled by the lifting and lowering of the moving plunger by external power, and the nucleic acid of pathogenic bacteria can be extracted, amplified/hybridized, quantified by homogenization, and then passed through the corresponding detection chamber. The detection hole is used for multiple index detection.
本实用新型所述柱塞式核酸检测一体化卡盒结构简单，能够解决病菌检测存在交叉污染、以及人员操作失误造成的准确度不高的问题。本实用新型所述方法在卡盒中，能够完成病菌检测的整个过程，包括核酸提取、扩增/杂交和检测，具有很强的实用性，并且可通过外部动力控制实现自动化检测。The plunger-type nucleic acid detection integrated cartridge of the utility model has a simple structure, and can solve the problems of cross-contamination in the detection of germs and low accuracy caused by operator errors. The method of the utility model can complete the whole process of bacteria detection in a cassette, including nucleic acid extraction, amplification/hybridization and detection, has strong practicability, and can realize automatic detection through external power control.
附图说明Description of drawings
图1是本实用新型所述一体化卡盒表面结构图；Fig. 1 is the surface structure diagram of the integrated cassette of the present invention;
图2是本实用新型所述一体化卡盒底部结构图；Fig. 2 is the bottom structure diagram of the integrated cassette of the present invention;
图3是本实用新型所述一体化卡盒内部结构图；Fig. 3 is the internal structure diagram of the integrated cassette of the present invention;
图4是本实用新型所述一体化卡盒内部结构图。FIG. 4 is an internal structure diagram of the integrated cartridge according to the present invention.
其中，1、反应室；2、试剂室；3、均液室；4、检测室；5流体双向阀；6、流体单向阀；7、磁铁；8、加热器；9、活动柱塞；10、变温器。Among them, 1. reaction chamber; 2. reagent chamber; 3. liquid homogenization chamber; 4. detection chamber; 5. fluid two-way valve; 6. fluid one-way valve; 7. magnet; 8. heater; 9. movable plunger; 10. Thermostat.
具体实施方式Detailed ways
下面结合实施例进一步解释和阐明，但具体实施例并不对本实用新型有任何形式的限定。若未特别指明，实施例中所用的方法和设备为本领常规方法和设备，所用原料均为常规市售原料。The following is further explained and illustrated in conjunction with the examples, but the specific examples do not limit the present invention in any form. Unless otherwise specified, the methods and equipment used in the examples are conventional methods and equipment in the art, and the raw materials used are conventional commercially available raw materials.
实施例1Example 1
如图1~4所示，一种柱塞式核酸检测一体化卡盒，包括一个反应室1、六个试剂室2、五个均液室3、五个检测室4和活动柱塞9，所述反应室组、试剂室组、均液室组、检测室组为卡盒内置的中空腔体，腔体的开口在卡盒表面，所述活动柱塞9与反应室1、试剂室2、均液室3和检测室4的腔体相匹配，活动柱塞9可借助外部动力在腔体内上拉或下推；所述反应室1与试剂室2通过管道连接，同时反应室1通过管道与均液室3其中的1个连接。均液室3由相同水平高度的管道连接，均液室3与检测室4在底部通过管道对应连接。在反应室1和各个试剂室2的管道入口设有流体双向阀门5，均液室3与其对应连接的检测室4之间设有流体单向阀门6。均液室3之间设有阀门。As shown in Figures 1-4, a plunger-type nucleic acid detection integrated cartridge includes a
所述反应室1底部设有磁铁7和加热器8，所述磁铁7可将磁性物质吸附在反应室1底部，防止磁性物质流失或对检测造成影响。The bottom of the
所述均液室3和检测室4底部设有变温器10。底部的加热器8和变温器10能够对反应溶剂进行温度调节。A
所述外部动力由电机、气泵、液泵的一种或多种方式提供。可通过微处理器对外部动力的控制，实现分子（核酸、蛋白质等）自动化检测。The external power is provided by one or more ways of a motor, an air pump, and a liquid pump. The automatic detection of molecules (nucleic acids, proteins, etc.) can be realized through the control of the external power by the microprocessor.
所述卡盒还设有检测孔，所述检测孔可以对检测室内的溶液采用对射、散射、反射的一种或多种的检测，例如荧光检测、紫外线检测、浊度检测等光学检测。The cartridge is further provided with a detection hole, which can detect one or more of the solution in the detection chamber by radiation, scattering, and reflection, such as optical detection such as fluorescence detection, ultraviolet detection, and turbidity detection.
实施例2Example 2
一种柱塞式核酸检测一体化卡盒，包括一个废液室、二个反应室、六个试剂室、三个均液室、三个检测室和活动柱塞，所述反应室、试剂室、均液室和检测室组为卡盒内置的中空腔体，腔体的开口在卡盒表面，所述活动柱塞与反应室组、试剂室组、均液室组和检测室组的腔体相匹配，活动柱塞可借助外部动力在腔体内上拉或下推。所述反应室与废液室连接，反应室与试剂室组通过管道连接，同时反应室通过管道与均液室其中的一个连接。均液室由相同水平高度的管道连接，均液室与检测室在底部通过管道对应连接。在反应室和各个试剂室的管道入口设有流体双向阀门，均液室与其对应连接的检测室之间设有流体单向阀门，反应室和废液室之间设有流体单向阀门。A plunger-type nucleic acid detection integrated cartridge, comprising a waste liquid chamber, two reaction chambers, six reagent chambers, three liquid homogenization chambers, three detection chambers and a movable plunger, the reaction chambers, the reagent chambers , The liquid homogenization chamber and the detection chamber group are hollow chambers built into the cartridge, the opening of the cavity is on the surface of the cartridge, and the movable plunger is connected to the chambers of the reaction chamber group, the reagent chamber group, the liquid equalization chamber group and the detection chamber group. The movable plunger can be pulled up or pushed down in the cavity with the help of external power. The reaction chamber is connected with the waste liquid chamber, the reaction chamber is connected with the reagent chamber group through a pipeline, and the reaction chamber is connected with one of the liquid homogenization chambers through a pipeline. The liquid homogenization chambers are connected by pipes of the same level, and the liquid homogenization chamber and the detection chamber are connected correspondingly through pipes at the bottom. A fluid two-way valve is arranged at the pipeline inlet of the reaction chamber and each reagent chamber, a fluid one-way valve is arranged between the liquid homogenization chamber and its correspondingly connected detection chamber, and a fluid one-way valve is arranged between the reaction chamber and the waste liquid chamber.
所述反应室底部设有磁铁和加热器，所述磁铁可将磁性物质吸附在反应室底部，防止磁性物质流失或对检测造成影响。The bottom of the reaction chamber is provided with a magnet and a heater, and the magnet can adsorb the magnetic substance on the bottom of the reaction chamber to prevent the loss of the magnetic substance or affect the detection.
所述均液室和检测室底部设有变温器。底部的加热器和变温器能够对反应溶剂进行温度调节。A temperature changer is provided at the bottom of the liquid homogenization chamber and the detection chamber. The bottom heater and temperature changer can adjust the temperature of the reaction solvent.
所述外部动力由电机、气泵、液泵的一种或多种方式提供。可通过微处理器对外部动力的控制，实现分子（核酸、蛋白质等）自动化检测。The external power is provided by one or more ways of a motor, an air pump, and a liquid pump. The automatic detection of molecules (nucleic acids, proteins, etc.) can be realized through the control of the external power by the microprocessor.
所述卡盒还设有检测孔。所述检测孔可以对检测室内的溶液采用对射、散射、反射的一种或多种的检测，例如荧光检测、紫外线检测、浊度检测等光学检测。The cassette is also provided with a detection hole. The detection hole can use one or more detections of radiation, scattering, and reflection for the solution in the detection chamber, such as optical detection such as fluorescence detection, ultraviolet detection, and turbidity detection.
上述实施例仅仅为清楚地说明本实用新型所作的举例，而并非是对本实用新型的实施方式的限定。对于所属领域的普通技术人员来说，在上述说明的基础上还可以根据检测的需要，对反应室、试剂室、均液室和检测室进行个数、体积大小及位置关系的调整。The above-mentioned embodiments are only examples for clearly illustrating the present invention, and are not intended to limit the embodiments of the present invention. For those of ordinary skill in the art, on the basis of the above description, the number, volume and positional relationship of the reaction chambers, reagent chambers, homogenization chambers and detection chambers can also be adjusted according to the needs of detection.
实施例3Example 3
本实施提供实施例所述柱塞式核酸检测一体化卡盒的检测方法，其步骤包括：This implementation provides the detection method of the plunger-type nucleic acid detection integrated cartridge described in the embodiment, the steps of which include:
S1.将所需试剂、磁性物质和检测标记物等液体或固体物质分别事先封存进试剂室组和检测室组，活动柱塞密封，再将被检测物放入反应室中，塞上活动柱塞；S1. Store liquid or solid substances such as required reagents, magnetic substances and detection markers in the reagent chamber group and detection chamber group respectively in advance, seal the movable plunger, and then put the detected object into the reaction chamber and plug the movable column plug;
S2.将试剂组中的反应试剂和磁性物质分别通过活动柱塞压入反应室中与被检测物，加热、混匀，通过反应室内活动柱塞和溶剂室的活动柱塞的上拉与下推实现溶液的混匀。S2. Press the reaction reagents and magnetic substances in the reagent group into the reaction chamber through the movable plunger and the detected object respectively, heat and mix, and then pull up and down through the movable plunger in the reaction chamber and the movable plunger in the solvent chamber. Push to mix the solution.
S3.反应完后，通过反应室底部的磁性物质将被检测物吸附在反应室，将反应试剂通过活动柱塞压入原溶剂存放室或废液室；S3. After the reaction is completed, the detected object is adsorbed in the reaction chamber through the magnetic substance at the bottom of the reaction chamber, and the reaction reagent is pressed into the original solvent storage chamber or the waste liquid chamber through the movable plunger;
S4.重复步骤S2~3将各溶剂压入反应室操作，对核酸进行提纯、扩增、杂交的一种或多种，将被检测物与溶剂混合均匀后，将混合溶液压入均液室组均匀分液；S4. Repeat steps S2-3 to press each solvent into the reaction chamber to perform one or more of nucleic acid purification, amplification, and hybridization. After mixing the detected substance and the solvent evenly, press the mixed solution into the homogenizing chamber. The components are evenly divided;
S5.将分液室中的混合液压入与之对应的检测室组中，通过检测孔对混合液体进行多重定量和/或定性检测。S5. Put the mixed liquid in the liquid separation chamber into the corresponding detection chamber group, and perform multiple quantitative and/or qualitative detection on the mixed liquid through the detection holes.
显然，本实用新型的上述实施例仅仅是为清楚地说明本实用新型所作的举例，而并非是对本实用新型的实施方式的限定。对于所属领域的普通技术人员来说，在上述说明的基础上还可以做出其它不同形式的变化或变动。这里无需也无法对所有的实施方式予以穷举。凡在本实用新型的精神和原则之内所作的任何修改、等同替换和改进等，均应包含在本实用新型权利要求的保护范围之内。Obviously, the above-mentioned embodiments of the present invention are only examples for clearly illustrating the present invention, rather than limiting the implementation of the present invention. For those of ordinary skill in the art, changes or modifications in other different forms can also be made on the basis of the above description. There is no need and cannot be exhaustive of all implementations here. Any modifications, equivalent replacements and improvements made within the spirit and principles of the present utility model shall be included within the protection scope of the claims of the present utility model.
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