CN210953816U - Fluorescence detection system - Google Patents

Abstract

The application discloses a fluorescence detection system, which comprises one or more optical modules, a light transmission device, one or more sample storages and a driving device. The optical module comprises a light source and a fluorescence detection device which are matched, the light transmission device comprises one or more light transmission pieces, the sample storage is made of transparent plastic or glass materials, and the driving device comprises a motor and a transmission part. One end of the light transmission piece is matched with the optical module, and the other end of the light transmission piece is matched with the sample storage. Through setting up a plurality of assorted fluorescence detection device and light source, ensure to send the light of different wavelength and detect the material of different grade type, rethread drive arrangement drive optical module and/or sample memory and/or light transmission piece remove, realize carrying out the material detection of polymorphic type to the determinand in a plurality of sample memories simultaneously.

Description

Fluorescence detection system

Technical Field

The utility model relates to a biological class detects technical field, especially relates to a fluorescence detecting system.

Background

The following are known to the applicant and do not necessarily become known techniques.

The fluorescence detection system has high sensitivity and good selectivity, so the fluorescence detection system is always an important biological detection technology. In most biological detection applications, it is often desirable to measure multiple biological samples and multiple wavelengths simultaneously. To achieve such multi-sample multi-wavelength detection, the existing fluorescence detection system generally adopts one of the following two methods: 1) the photographic technique comprises the following steps: the same broad-spectrum light source is adopted, excitation light with required wavelength is obtained through a light filter with specific wavelength, and then reaction fluorescence of all samples is collected simultaneously by adopting a photographic technology (CCD). Then, the filters with different wavelengths are switched to obtain the reaction fluorescent signals with different wavelengths. 2) Scanning method: similar to the photographic technology, the same broad-spectrum light source is adopted, the excitation light with the required wavelength is obtained through a filter with a specific wavelength, but the detection element is a single photoelectric sensor, only one sample is measured each time, and then the light source and the detection module are mechanically moved to measure the next sample. The switching between different wavelengths is also done by mechanically switching the filters, which can be switched to the next sample after the measurement of all wavelengths of the same sample is done, or the filters can be switched after the measurement of all samples at the same wavelength is done, and then all samples are scanned repeatedly.

In the above conventional methods, the photographic technique is fast, but the cost is high; the scanning method is low in cost, but the detection speed is slow.

SUMMERY OF THE UTILITY MODEL

The embodiment of the utility model provides a problem to be solved provides a fluorescence detection system with low costs.

On the one hand, the fluorescence detection system comprises one or more optical modules, a driving device, a light transmission device and one or more sample storages, wherein each optical module comprises a light source and the fluorescence detection device which are matched, the driving device is used for driving the optical modules and/or the sample storages and/or the light transmission device, the light transmission device comprises one or more light transmission pieces, one end of each light transmission piece is matched with the optical module, and the other end of each light transmission piece is matched with the sample storages.

In one possible embodiment, the drive device is used to drive the optical modules and/or the sample storage devices and/or the light transmission device such that one end of the light transmission element cooperates with one or more optical modules, and/or the drive device is used to drive the optical modules and/or the sample storage devices and/or the light transmission device such that the other end of the light transmission element cooperates with one or more sample storage devices.

In one possible embodiment, when one end of the light transmission member is opposite to the optical module and the other end is opposite to the sample storage, the light emitted by the light source in the optical module is transmitted to the corresponding sample storage through the light transmission device, and the light emitted by the sample in the sample storage is transmitted back to the fluorescence detection device corresponding to the light source through the light transmission device.

In one possible embodiment, when one end of the light transmission member corresponds to the output end of the light source, one end of the light transmission member corresponds to the input end of the fluorescence detection device matched with the light source; in one possible embodiment, the other end of the light transmission member corresponds to the sample storage.

In one possible embodiment, the light transmitting member is an optical fiber.

In one possible embodiment, the movement track of the other end of the light transmission piece driven by the driving device is matched with the distribution position of the sample storage; and/or the driving device drives the moving track of one end of the light transmission piece to move to be matched with the distribution position of the output end of the light source; and/or the driving device drives the moving track of one end of the light transmission piece to move to be matched with the distribution position of the input end of the fluorescence detection device; and/or the movement track of the sample storage driven by the driving device is matched with the distribution position of the other end of the light transmission piece; and/or the moving track of the light source and/or the fluorescence detection device driven by the driving device is matched with the distribution position of one end of the light transmission piece;

in one possible embodiment, the movement track of the sample storage and/or the light transmission member and/or the optical module, which is driven by the driving device, is circular, square, elliptical or polygonal; optimally, the shape is circular;

in one possible embodiment, the distribution locus of the one or more sample storages and/or light transmission elements and/or optical modules is circular, square, elliptical or polygonal; optimally, the shape is circular;

in one possible embodiment, the distribution trajectory of the one or more sample storages and/or light transmission elements and/or optical modules is adapted to the movement trajectory of the drive device.

In one possible embodiment, the optical module further includes a light splitting device, and the light splitting device is used for changing paths of light with different wavelengths;

in one possible embodiment, the light splitting device is located between the output end of the light source and one end of the light transmission member, and more preferably, the light emitted by the light source reaches one end of the light transmission member through the light splitting device;

in one possible embodiment, the light splitting device is located between the input end of the fluorescence detection device and one end of the light transmission member, and more preferably, the light emitted from one end of the light transmission member reaches the input end of the fluorescence detection device through the light splitting device;

in one possible embodiment, the light splitting device is one or more of a spectroscope and a dichroic mirror, and preferably, the light splitting device is a prism spectroscope or a grating spectroscope.

In one possible embodiment, the fluorescence detection device is provided with a first filtering device for filtering clutter; preferably, the wavelength of the light filtered by the first filtering device in the fluorescence detection device is the same as the wavelength of the output light of the light source matched with the wavelength of the filtered light; preferably, the first filtering device is arranged between the fluorescence detection device and one end of the light transmission piece;

or the light source is provided with a second filtering device, and the second filtering device is used for filtering clutter; preferably, the wavelength of the light filtered by the second filter device in the fluorescence detection device is the same as the wavelength of the output light of the light source matched with the wavelength of the filtered light; preferably, the second filtering device is arranged between the fluorescence detection device and one end of the light transmission piece;

or the fluorescence detection system further comprises a third filtering device, wherein the third filtering device is used for filtering clutter and is arranged between the other end of the light transmission piece and the sample storage;

in one possible embodiment, the first filtering device, the second filtering device and the third filtering device are filters, and most preferably one or more of a band-pass filter, a cut filter, a spectral filter, a neutral density filter, a reflective filter and a thin film filter.

In one possible embodiment, the output light of at least two light sources has different wavelengths;

preferably, the output light of different light sources is of different wavelengths.

In one possible embodiment, the fluorescence detection system comprises a module fixing disc, the module fixing disc is used for fixing each optical module, and the driving device is used for driving each optical module to move through driving the module fixing disc;

or the light transmission device comprises a transmission piece fixing disc, the transmission piece fixing disc is used for fixing each light transmission piece, and the driving device is used for driving each light transmission piece to move by driving the fixing disc;

or comprises a memory fixing disc used for fixing each sample memory, and the driving device is used for driving each sample memory to move by driving the memory fixing disc.

In one possible embodiment, the driving device is one or more of an electric motor, a pneumatic cylinder and a motor, preferably one or more of a rotary pneumatic cylinder, a rotary electric motor and a rotary motor.

Compared with the prior art, the technical scheme has the advantages that:

(1) firstly, the fluorescence detection device and the light source are combined in one optical module, each optical module comprises one wavelength, and a user can flexibly replace or add the optical modules according to needs to realize single specific wavelength or multi-wavelength detection, so that the scheme has good expansibility and pertinence.

(2) The detection efficiency of the scheme is high, a plurality of sample storages can be detected simultaneously, and the content of various substances can be detected simultaneously.

(3) In addition, the scheme transmits incident light and reflected light (fluorescence) through the light transmission piece, so that the complexity of the whole system is reduced, and the cost is reduced.

Drawings

Fig. 1 is a schematic structural view of embodiment 1.

Fig. 2 is a schematic structural view of embodiment 2.

Fig. 3 is a schematic structural view of embodiment 3.

FIG. 4 is a diagram illustrating the distribution of sample memories according to one embodiment.

FIG. 5 is a schematic diagram of the distribution of optical modules in one embodiment.

The labels in the figure are: 1. a light transmission device; 2. an optical module; 21. a fluorescence detection device; 22. a light source; 3. a sample reservoir; 4. a drive device; 5. a light splitting device; 6. a first filtering means; 7. a second filtering means; 8. and a third filtering device.

Detailed Description

To further illustrate the technical means and effects of the present invention adopted to achieve the intended purpose of the present invention, the following detailed description is given to the embodiments, structures, features and effects according to the present invention with reference to the accompanying drawings and preferred embodiments as follows: it is to be understood that the terms "plurality," "plurality," and the like are two or more unless otherwise specified.

Example 1

As shown in fig. 1, the fluorescence detection system comprises one or more optical modules 2, a driving device 4, a light transmission device 1 and one or more sample storages 3, wherein the optical module 2 comprises a light source 22 and a fluorescence detection device 21 which are matched, the driving device 4 is used for driving the sample storages 3 and/or light transmission members to move, the light transmission device 1 comprises one or more light transmission members, one end of each light transmission member is matched with the optical module 2, the other end of each light transmission member is matched with the sample storages 3 (it should be understood that one end of each light transmission member is matched with the optical module 2, that is, one end of each light transmission member can be matched with one or more optical modules 2 (through rotation), the other end of each light transmission member is matched with the sample storages 3, that is, the other end of each light transmission member can be matched with one or more sample storages 3 (through, in the present application, the number of matching is not limited, that is, at a certain time, one or more optical modules 2 can be matched with one light transmission member and one or more sample storages, so that the light emitted from the optical modules 2 is transmitted to the corresponding sample storages 3 through the light transmission device, and the light emitted from the samples in the sample storages is transmitted back to the fluorescence detection device corresponding to the light source by the light transmission device. It should be understood that normally one optical module 2 is matched to one sample holder 3 at the same time, although i do not exclude that one or more optical modules are matched to one or more sample holders 3 at the same time. ). The fluorescence detection device 21 is used for detecting fluorescence coming back from the sample storage 3, thereby detecting the content of the corresponding substance. It should be understood that the sample storage 3 may be a vessel such as a beaker, a sample bottle, or the like for storing the analyte, and may also be an object such as a reactor that can be used for reaction.

In the above basic technical solution, by setting a plurality of matched fluorescence detection devices 21 and light sources 22, it is ensured that light with different wavelengths can be emitted and different types of substances can be detected, and then the driving device 4 drives the sample storage 3 and/or the light transmission member to move, so as to realize simultaneous multi-type substance detection on samples (i.e. objects to be detected) in the plurality of sample storage 3, thereby detecting the content of each substance in the objects to be detected in a short time and accelerating the detection speed. Moreover, the above solution reduces the complexity of the whole device by placing the light source 22 and the fluorescence detection device 21 at the same time at one end of the light transmission member, so that only one light transmission member (preferably an optical fiber in this embodiment) is required to simultaneously satisfy the requirement of light reaching one sample storage 3 and fluorescence returning from one sample storage 3 to the fluorescence detection device 21. It should be understood that in the above basic solution, the number of the optical modules 2 and the number of the sample storages 3 may not correspond to one another.

In one possible embodiment, the drive means 4 are used to drive the sample holders 3 and/or the fluorescence detection means 21 such that one end of the light transmissive element cooperates with one or more optical modules 2, and/or the drive means 4 are used to drive the sample holders 3 and/or the fluorescence detection means 21 such that the other end of the light transmissive element cooperates with one or more sample holders 3. It will be appreciated that the light transmissive element comprises at least two ports, preferably two ports.

In one possible embodiment, when one end of the light transmission member is opposite to the optical module 2 and the other end is opposite to the sample storage 3, the light emitted from the light source 22 in the optical module 2 is transmitted to the corresponding sample storage 3 through the light transmission device 1, and the light emitted from the sample storage 3 is transmitted back to the fluorescence detection device 21 corresponding to the light source 22 by the light transmission device 1.

In one possible embodiment, one end of the light transmitting member corresponds to the input end of the fluorescence detecting device 21 matching the light source 22, while the other end of the light transmitting member corresponds to the output end of the light source 22. While the other end of the light transmitting member corresponds to a sample reservoir 3.

In order to ensure that one end of the light transmission member can correspond to the position of the other optical module 2 and the other end can correspond to the position of the other sample storage 3 after the driving device 4 drives the sample storage 3 and/or the light transmission member to move, the following design is provided:

in one possible embodiment, the driving device 4 drives the other end of the light transmission member to move along a moving track matched with the distribution position of the sample storage 3; and/or the driving device 4 drives the moving track of one end of the light transmission member to move to match with the distribution position of the output end of the light source 22; and/or the driving device 4 drives one end of the light transmission member to move along a moving track matched with the distribution position of the input end of the fluorescence detection device 21.

In one possible embodiment, the movement track of the sample storage 3 and/or the light transmission member is driven by the driving device 4 to move in a circular, square, elliptical or polygonal shape; optimally, the shape is circular;

in one of the possible embodiments, the distribution locus of the plurality of sample storages 3 is a circle, a square, an ellipse, or a polygon; optimally, the shape is circular;

in one of the possible embodiments, the distribution trajectory of the plurality of sample storages 3 is matched to the movement trajectory of the drive device 4.

In one possible embodiment, the optical module 2 further includes a light splitting device 5, the light splitting device 5 is located between one end of the light transmission member and the optical module 2, and the light splitting device 5 is used for changing paths of light with different wavelengths;

in one possible embodiment, the light splitting device 5 is located between the output end of the light source 22 and one end of the light transmission member, and more preferably, the light emitted from the light source 22 reaches one end of the light transmission member through the light splitting device 5;

in one possible embodiment, the light splitting device 5 is located between the input end of the fluorescence detection device 21 and one end of the light transmission member, and more preferably, the light emitted from one end of the light transmission member reaches the input end of the fluorescence detection device 21 through the light splitting device 5;

it should be understood that by arranging the light splitting device 5, the incident light entering from one end of the light transmission member and the reflected light (fluorescence) coming out from one end of the light transmission member can travel different optical paths, that is, the light with the same wavelength as the incident light is reflected, and the light with the different wavelength from the incident light (especially the light with the same wavelength as the fluorescence) directly passes through the light splitting device 5. Therefore, one can associate the matched fluorescence detecting means 21 with the positions of the light source 22 and one end of the light transmitting member, so that it is possible to accomplish both the transmission of the incident light from the light source 22 to the sample storage 3 and the transmission of the reflected light from the sample storage 3 to the fluorescence detecting means 21 with only one light transmitting member. The complexity of the whole device is greatly reduced.

In one possible embodiment, the light splitting device 5 is one or more of a spectroscope and a dichroic mirror, and preferably, may be a prism spectroscope or a grating spectroscope.

In one of the possible embodiments, the fluorescence detection device 21 is provided with a first filtering device 6, the first filtering device 6 being used for filtering clutter; preferably, the wavelength of the light filtered by the first filter 6 in the fluorescence detection device 21 is the same as the wavelength of the output light of the light source 22; preferably, the first filter device 6 is arranged between the fluorescence detection device 21 and one end of the light transmission member;

or the light source 22 is provided with a second filtering device 7, and the second filtering device 7 is used for filtering clutter; preferably, the wavelength of the light filtered by the second filter 7 in the fluorescence detection device 21 is the same as the wavelength of the output light of the light source 22; preferably, the second filter device 7 is arranged between the fluorescence detection device 21 and one end of the light transmission member;

in one possible embodiment, the first filtering device 6 and the second filtering device 7 are filters, and most preferably one or more of a band-pass filter, a cut filter, a spectral filter, a neutral density filter, a reflective filter, and a thin film filter.

In one possible embodiment, the output light of at least two light sources 22 is of different wavelength; preferably, the output light of different light sources 22 is of different wavelengths.

In one possible embodiment, the device comprises a transmission member fixing disc, wherein the transmission member fixing disc is used for fixing each light transmission member, and the driving device 4 is used for driving each light transmission member to move by driving the fixing disc;

or a memory fixing disk for fixing each sample memory 3, and the driving device 4 for driving each sample memory 3 to move by driving the memory fixing disk.

In one possible embodiment, the driving device 4 is one or more of an electric motor, a pneumatic cylinder, and a motor, preferably one or more of a rotary pneumatic cylinder, a rotary electric machine, and a rotary motor.

In one possible embodiment, the material of the sample storage 3 is a transparent material, and preferably, the sample storage 3 is made of a transparent plastic or glass material.

In one possible embodiment, one end of one light-transmitting member corresponds to one optical module 2, and more preferably, one end of one light-transmitting member corresponds to one light source 22, and one end of the light-transmitting member also corresponds to one fluorescence detection device 21.

In one possible embodiment, the other end of one light transmitting member corresponds to one sample reservoir 3.

It should be understood that the light source 22 may be an LED light fixture, a laser emitting device, or the like. The fluorescence detection device 21 is used for receiving the reflected light and calculating the content of the substance contained in the analyte, and may be a combination of a photoelectric sensor and an analysis detection circuit.

In this embodiment, the driving device 4 is used to drive the sample storage 3 to rotate, as shown in fig. 1, the sample storage 3 is fixed on the storage fixing disk, and the driving device 4 drives the sample storage 3 to rotate by driving the storage fixing disk to rotate, wherein the sample storage 3 is arranged to form a circle with the rotation center as the center. The other ends of the corresponding light transmission members also enclose a parallel circle with the same size, and the connection line of the centers of the two circles is perpendicular to the rotation plane (i.e. the horizontal plane in this embodiment) of the sample storage 3.

When detecting, optical module 2 corresponds with a sample memory 3 and light transmission piece position, a light source 22 sends incident light, reaches the beam splitter behind the light filter, and under the effect of beam splitter, the laser of this wavelength can only take place the reflection to enter into light transmission piece through the one end of light transmission piece, then come out at the other end of light transmission piece, reach a sample memory 3, arouse the fluorescent substance in the determinand in a sample memory 3, make it send fluorescence. Meanwhile, the reflected fluorescent light enters the light transmission member through the other end of the light transmission member and exits from one end of the light transmission member. The reflected light of the fluorescence information reaches the spectroscopic plate, and the reflected light directly passes through the spectroscopic plate (without emission) because the wavelength of the reflected light is different from that of the incident light (fluorescence), and directly enters the first fluorescence detection device 21. Then, the sample storage 3 and/or the light transmission member are/is rotated, so that the second sample storage 3 corresponds to the first optical module 2, i.e. the laser of the first light source 22 can reach the second sample storage 3, and the fluorescence from the second sample storage 3 can return to the first fluorescence detection device 21. Meanwhile, the sample storage 3 can be corresponding to the positions of other optical modules 2 and can be detected by other optical modules 2. It will be appreciated that the number of sample holders 3 and the number of optical modules 2 need not be the same. The number of the sample storages 3 may be larger than the number of the optical modules 2, or the number of the optical modules 2 may be larger than the number of the sample storages 3.

It will be appreciated that the present application is also provided with fixing means (not shown) for fixing the light transmissive member to ensure that one end of the light transmissive member is aligned with the optical module 2 and one end is aligned with the sample holder 3.

Example 2

Unlike embodiment 1, as shown in fig. 2, in this embodiment, the optical module 2 is driven to rotate instead of the sample storage device 3, that is, the driving device 4 drives the optical module 2 (i.e., the light source 22 and/or the fluorescence detection device 21) to move along a moving track matched with the distribution position of one end of the light transmission member, so that when the optical module 2 rotates to the position corresponding to the optical transmission member, the output light source 22 of the light source 22 can be transmitted to the sample storage device 3 through the light transmission member, and the fluorescence of the sample storage device 3 can be transmitted back to the fluorescence detection device 21 through the light transmission member.

In one possible embodiment, the driving device 4 drives the optical module 2 (i.e. including the light source 22 and/or the fluorescence detection device 21) to move along a moving track matched with the distribution position of one end of the light transmission member;

in one possible embodiment, the movement track of the optical module 2 driven by the driving device 4 is circular, square, elliptical or polygonal; optimally, the shape is circular;

in one of the possible embodiments, the distribution locus of the plurality of sample storages 3 is a circle, a square, an ellipse, or a polygon; optimally, the shape is circular;

in one of the possible embodiments, the distribution trajectory of the sample holders 3 is matched to the movement trajectory of the optical module 2.

In one possible embodiment, the fluorescence detection system comprises a module fixing disc for fixing each optical module 2, and the driving device 4 is used for driving each optical module 2 to move by driving the module fixing disc;

in addition, in the present embodiment, the optical module 2 is placed on the module fixing tray. The rotating disc is provided with a driving device 4, the driving device 4 drives the module fixing disc to rotate, so as to drive the optical module 2 to rotate together, as shown in fig. 5, the optical module 2 surrounds a circle by taking the rotation center as the center of the circle. One end of each of the corresponding light transmission members also encloses a parallel circle with the same size, and a connection line of centers of the two circles is perpendicular to a rotation plane (i.e., a horizontal plane in this embodiment) of the optical module 2. Meanwhile, the sample storage 3 is fixed on the fixing plate as shown in fig. 4, and also encloses a circle of the same size.

The advantage of this embodiment over embodiment 1 is that the drive means 4 is prevented from moving the sample reservoir 3 so that the liquid in the sample reservoir 3 is relatively still and does not cause the liquid in the sample reservoir 3 to stir, which would affect the accuracy of the measurement. In addition, the movement of an optical transmission part (such as an optical fiber) is avoided, and the influence of the vibration of the optical fiber alignment head on the detection precision is reduced.

Example 3

Unlike embodiment 1, as shown in fig. 3, in one possible embodiment, the fluorescence detection system further comprises a third filtering device 8, the third filtering device 8 is used for filtering noise, the third filtering device 8 is arranged between the other end of the light transmission member and the sample storage 3, and preferably, the third filtering device 8 is a filter, and most preferably, the third filtering device is one or more of a band-pass filter, a cut-off filter, a beam splitter filter, a neutral density filter, a reflection filter and a thin film filter. It will be appreciated that the third filter means 8 may be secured between the other end of the light transmissive member and the sample reservoir 3 by some fixing means.

It should be understood that in the present embodiment, the third filtering device 8 is added between the other end of the light transmission member and the sample storage 3, so that after the light is emitted from the light source 22 excitation device, the light directly enters the light transmission member through the light splitter, and after the light comes out from the other end of the light transmission member, the light passes through the third filtering device, thereby avoiding the influence of other stray light on the fluorescence detection effect. The fluorescence excited by the analyte in the sample storage 3 also passes through the third filter device, and then passes through the light transmission member and the spectroscope to return to the fluorescence detection device 21. By adopting the mode, each optical module 2 can be provided with only one filtering device, so that the cost is reduced.

The above embodiments are only preferred embodiments of the present invention, and the protection scope of the present invention cannot be limited thereby, and any insubstantial changes and substitutions made by those skilled in the art based on the present invention are all within the protection scope of the present invention.

Claims (37)

1. A fluorescence detection system is characterized by comprising one or more optical modules, a light transmission device and a driving device, wherein each optical module comprises a light source and a fluorescence detection device which are matched, the light transmission device comprises one or more light transmission pieces, and the driving device is used for driving the optical modules and/or the light transmission device.

2. A fluorescence detection system according to claim 1, further comprising one or more sample reservoirs, wherein the drive means is arranged to drive the one or more sample reservoirs.

3. A fluorescence detection system according to claim 2, wherein the light transmissive member has one end adapted to engage the optical module and another end adapted to engage the sample holder.

4. A fluorescence detection system according to claim 2, wherein said drive means is adapted to drive the optical module and/or the sample holder and/or the light transmission means such that one end of the light transmission member cooperates with one or more optical modules, and/or wherein said drive means is adapted to drive the optical module and/or the sample holder and/or the light transmission means such that the other end of the light transmission member cooperates with one or more sample holders.

5. The fluorescence detecting system according to claim 2, wherein when the light transmitting member has one end facing the optical module and the other end facing the sample storage, the light emitted from the light source in the optical module is transmitted to the corresponding sample storage via the light transmitting device, and the light emitted from the sample in the sample storage is transmitted back to the fluorescence detecting device corresponding to the light source by the light transmitting device.

6. The fluorescence detection system of claim 1, wherein an end of the light transmissive member corresponds to an input end of a fluorescence detection device that mates with the light source when the end of the light transmissive member corresponds to the output end of the light source.

7. A fluorescence detection system according to claim 5, wherein the other end of the light transmissive member corresponds to the sample reservoir.

8. A fluorescence detection system according to claim 1, wherein said light transmissive member is an optical fiber.

9. A fluorescence detection system according to claim 2,

the driving device drives the moving track of the other end of the light transmission piece to move to be matched with the distribution position of the sample storage;

and/or the driving device drives the moving track of one end of the light transmission piece to move to be matched with the distribution position of the output end of the light source,

and/or the driving device drives the moving track of one end of the light transmission piece to move to be matched with the distribution position of the input end of the fluorescence detection device;

and/or the movement track of the sample storage driven by the driving device is matched with the distribution position of the other end of the light transmission piece;

and/or the driving device drives the light source and/or the fluorescence detection device to move along a moving track matched with the distribution position of one end of the light transmission piece.

10. A fluorescence detection system according to claim 2, wherein the movement locus along which the driving means drives the sample storage and/or the light transmission member and/or the optical module to move is circular, square, elliptical or polygonal.

11. A fluorescence detection system according to claim 2, wherein the distribution locus of the one or more sample reservoirs and/or the light transmissive member and/or the optical module is circular, square, elliptical or polygonal.

12. A fluorescence detection system according to claim 2, wherein the distribution locus of the one or more sample storage devices and/or the light transmissive element and/or the optical module matches the movement locus of the light transmissive element and/or the optical module driven by the driving means.

13. A fluorescence detection system according to claim 3, wherein the optical module further comprises beam splitting means for altering the path of light of different wavelengths.

14. A fluorescence detection system according to claim 13, wherein the light-splitting means is located between the output end of the light source and an end of the light transmitting member.

15. A fluorescence detection system according to claim 14, wherein light from the light source passes through the light-splitting means to one end of the light-transmissive member.

16. A fluorescence detection system according to claim 13, wherein the light-splitting means is located between the input end of the fluorescence detection means and an end of the light transmitting member.

17. A fluorescence detection system according to claim 16, wherein light emitted from an end of the light transmissive member passes through the light-splitting means to the input end of the fluorescence detection means.

18. A fluorescence detection system according to claim 13, wherein the spectroscopic means is one or more of a spectroscope, a dichroic mirror.

19. A fluorescence detection system according to claim 18, wherein said beam splitting means is a prism beam splitter or a grating beam splitter.

20. A fluorescence detection system according to claim 1, wherein the fluorescence detection means is provided with first filtering means for filtering clutter.

21. A fluorescence detection system according to claim 20, wherein the first filter means in the fluorescence detection means filters light having the same wavelength as the output light of the matched light source.

22. A fluorescence detection system according to claim 20, wherein said first filter means is disposed between the fluorescence detection means and an end of the light transmissive member.

23. A fluorescence detection system according to claim 20, wherein said first filter means is a filter.

24. The fluorescence detection system of claim 23, wherein the first filter means is one or more of a band pass filter, a cut filter, a spectral filter, a neutral density filter, a reflective filter, and a thin film filter.

25. A fluorescence detection system according to claim 1, wherein the light source is provided with second filtering means for filtering clutter.

26. A fluorescence detection system according to claim 25, wherein the wavelength of the light filtered by the second filter means in the fluorescence detection means is the same as the wavelength of the output light of the matched light source.

27. A fluorescence detection system according to claim 25, wherein said second filter means is disposed between the fluorescence detection means and an end of the light transmissive member.

28. A fluorescence detection system according to claim 25, wherein said second filter means is a filter.

29. A fluorescence detection system according to claim 28, wherein said second filter means is one or more of a band pass filter, a cut filter, a spectral filter, a neutral density filter, a reflective filter, and a thin film filter.

30. A fluorescence detection system according to claim 2, further comprising third filtering means for filtering noise, said third filtering means being disposed between the other end of the light transmitting member and the sample reservoir.

31. A fluorescence detection system according to claim 30, wherein said third filter means is a filter.

32. A fluorescence detection system according to claim 31, wherein said third filter means is one or more of a band pass filter, a cut filter, a spectral filter, a neutral density filter, a reflective filter, and a thin film filter.

33. A fluorescence detection system according to claim 1, wherein the output light of at least two of the light sources has a different wavelength.

34. A fluorescence detection system according to claim 33, wherein the output light from different light sources has different wavelengths.

35. A fluorescence detection system according to claim 2,

the optical module fixing device comprises a module fixing disc, a driving device and a module fixing disc, wherein the module fixing disc is used for fixing each optical module, and the driving device is used for driving each optical module to move through the driving module fixing disc;

or the driving device comprises a transmission piece fixing disc, the transmission piece fixing disc is used for fixing each light transmission piece, and the driving device is used for driving each light transmission piece to move by driving the fixing disc;

or comprises a memory fixing disc used for fixing each sample memory, and the driving device is used for driving each sample memory to move by driving the memory fixing disc.

36. The fluorescence detection system of claim 1, wherein the driving device is one or more of an electric motor, an air cylinder, and a motor.

37. A fluorescence detection system according to claim 35, wherein said driving means is one or more of a rotary air cylinder, a rotary electric motor, and a rotary electric motor.

Images