CN210803492U

CN210803492U - Kit

Info

Publication number: CN210803492U
Application number: CN201921706242.7U
Authority: CN
Inventors: 田克恭; 王莹; 邓均华
Original assignee: Luoyang Pu Tai Biotechnology Co ltd
Current assignee: Luoyang Pu Tai Biotechnology Co ltd
Priority date: 2019-10-12
Filing date: 2019-10-12
Publication date: 2020-06-19
Anticipated expiration: 2029-10-12

Abstract

A kit having a housing, the housing comprising: the liquid sealing pool is used for storing buffer liquid; each nitrocellulose membrane is in a strip shape, and an enzyme labeling pad, a detection line and a contrast line are sequentially arranged on the upper surface of each nitrocellulose membrane; the buffer pad, the first part is connected with the upper surface of one end of each nitrocellulose membrane, the second part is immersed in the buffer solution, the buffer solution can be conveyed to the nitrocellulose membrane through capillary action, and the buffer solution can sequentially pass through the enzyme labeling pad, the detection line and the comparison line along the nitrocellulose membrane; wherein, different enzyme labeling pads of different nitrocellulose membranes have enzyme thereinMonoclonal antibody Y of labeled different antigens₁And detecting monoclonal antibody Y with corresponding antigen in line₂A goat anti-mouse secondary antibody is arranged in the control line; antigen and corresponding monoclonal antibody Y₁And monoclonal antibody Y₂A color reaction occurs, monoclonal antibody Y₁And the antibody reacts with a goat anti-mouse secondary antibody in a color reaction manner. Therefore, multiple antigens in the sample can be detected simultaneously, and the detection process is more convenient and faster.

Description

Kit

Technical Field

The invention relates to the technical field of biology, in particular to a kit.

Background

At present, more and more people like to raise pets such as cats, dogs and the like, and the pets enable the life of people to be more full, thereby bringing much joy to people. However, pets carry a lot of viruses, which brings great danger to the health of pets and pet owners. In order to detect whether a pet carries dangerous viruses, samples such as blood of the pet and the like are generally extracted and then detected through various types of kits, so that the operation is very complicated. Therefore, the market needs a kit which can detect antigens such as various viruses and the like at the same time, so that the detection process is more convenient and faster.

Disclosure of Invention

In view of the above, the main objective of the present invention is to provide a kit for simultaneously detecting multiple antigens.

The invention provides a kit, which is provided with a shell, wherein the shell comprises: the liquid sealing pool is used for storing buffer liquid; each nitrocellulose membrane is in a strip shape, and an enzyme labeling pad, a detection line and a contrast line are sequentially arranged on the upper surface of each nitrocellulose membrane; the buffer pad, the first part is connected with the upper surface of one end of each nitrocellulose membrane, the second part is immersed in the buffer solution, the buffer solution can be conveyed to the nitrocellulose membrane through capillary action, and the buffer solution can sequentially pass through the enzyme labeling pad, the detection line and the comparison line along the nitrocellulose membrane; wherein, different nitrocellulose membranes have monoclonal antibodies Y of different enzyme-labeled antigens in different enzyme labeling pads₁And detecting monoclonal antibody Y with corresponding antigen in line₂A goat anti-mouse secondary antibody is arranged in the control line; antigen and corresponding monoclonal antibody Y₁And monoclonal antibody Y₂A color reaction occurs, monoclonal antibody Y₁And the antibody reacts with a goat anti-mouse secondary antibody in a color reaction manner.

Adopt as above structure, after dripping the sample on the ELIAS pad on a plurality of nitrocellulose membranes, can carry the material in the ELIAS pad through buffer solution simultaneously and detect line and control line on a plurality of nitrocellulose membranes to this detects the multiple antigen in the sample simultaneously, makes the process of detecting convenient and fast more.

Preferably, the shell is provided with a first space and a plurality of second spaces arranged around the first space; the buffer pad is arranged in the first space, the second part of the buffer pad is arranged corresponding to the first space, and the first part of the buffer pad comprises a plurality of extending parts which are arranged corresponding to the plurality of nitrocellulose membranes in an outward divergence mode.

Preferably, the housing is cylindrical; the first space is cylindrically arranged in the middle of the shell; the plurality of second spaces are fan-shaped spaces which are evenly divided outside the first space in the shell.

By adopting the structure, the detection process can be arranged in different second spaces, so that the influence among the detections is prevented, and the accuracy of the detection result is improved.

Preferably, the buffer sealing pool is provided with an opening sealed by a sealing film, and the other end of the buffer cushion can be immersed in buffer after breaking the sealing film.

By adopting the structure, the buffer solution is sealed and stored by the sealing film, and the buffer solution can be prevented from being spilled accidentally.

Preferably, the shell is provided with a button, the button is provided with a sharp part at a position corresponding to the other end of the buffer pad, and the sharp part can be abutted against the other end of the buffer pad to break the sealing membrane and then be immersed in the buffer solution by pressing the button.

Adopt like above structure, thereby destroy the seal membrane through the button pressing and make the other end of blotter dip in buffer solution, it is easy and simple to handle, make the process of detection convenient and fast more.

Preferably, the shell is provided with a sample hole at a position corresponding to the enzyme labeling pad, and an observation port is arranged at a position corresponding to the detection line and the control line.

By adopting the structure, the sample can be added more conveniently, and the detection result can be observed more conveniently.

Preferably, the housing is provided with a groove, and the sample well is located at the bottom of the groove.

By adopting the structure, only the sample is required to be dripped into the groove, and the sample holes are not required to be dripped one by one, so that the detection process is more convenient and faster.

Preferably, the other end of the nitrocellulose membrane is provided with a water absorption pad.

By adopting the structure, after the buffer solution reaches the water absorption pad through the enzyme labeling pad, the detection line and the control line, the water absorption pad can continuously absorb the buffer solution, so that the detection line and the control line can be fully contacted with substances carried out in the buffer solution enzyme labeling pad, and the detection accuracy can be improved.

Drawings

FIG. 1 is a schematic view showing a configuration in the axial direction of a cartridge according to a first embodiment;

FIG. 2 is a schematic structural diagram of the button of FIG. 1;

FIG. 3 is a schematic view of the internal structure of the reagent cartridge of FIG. 1;

FIG. 4 is a schematic view of the test strip of FIG. 3;

FIG. 5 is a schematic top view of the reagent cartridge of FIG. 1;

FIG. 6 is a cross-sectional view taken along line A-A of FIG. 5;

FIG. 7 is a schematic view showing the structure of the second embodiment in the axial direction of the cartridge.

Description of the reference numerals

A housing 1; a sample well 11; a viewing port 12; a first partition plate 13; a first space 131; a liquid seal tank 132; a sealing film 133; a second separator 14; the second space 141; a detection station 142; a pad attaching part 143; a first clamping portion 144; a second clamping portion 145; the third clamping portion 146; a groove 15; a button 2; an opening 21; a spike portion 22; a test strip 3; a bottom plate 31; a nitrocellulose membrane 311; an enzyme label pad 32; a detection line 33; a control line 34; a water-absorbing pad 4; a cushion pad 5.

Detailed Description

First embodiment

Next, the specific structure of the first embodiment will be described in detail with reference to the drawings.

Fig. 1 is a schematic view of the configuration of the first embodiment in the axial direction of the cartridge. As shown in fig. 1, the reagent cartridge of the first embodiment has a thin cylindrical shape as a whole, and specifically includes: a housing 1 in a thin cylindrical shape; a cylindrical button 2 protruding from the inside of the shell 1 is arranged in the middle of the top of the shell 1; eight round hole-shaped sample holes 11 are evenly distributed on the top of the shell 1 at the radius position of 1/2; a rectangular observation port 12 is provided at a position intermediate between the sample hole 11 and the edge of the case 1 in the radial direction. That is, the eight sample holes 11 and the eight observation ports 12 are respectively located on two concentric circles with different radii, and each sample hole 11 and its corresponding observation port 12 are located on the same radius line.

Fig. 2 is a schematic structural diagram of the button 2 in fig. 1. FIG. 3 is a schematic view of the internal structure of the reagent cartridge of FIG. 1. As shown in fig. 2, the button 2 is a hollow thin-walled structure, the bottom of the button has a circular opening 21, and the button 2 has a spike 22 extending vertically downward and protruding from the inside of the button 2. As shown in fig. 3, a circular first partition plate 13 concentric with the casing 1 is arranged in the casing 1 to divide the cylindrical space in the casing 1 into a smaller cylindrical first space 131 in the middle; a second partition 14 is provided between the first partition 13 and the edge of the casing 1 in the radial direction, and divides the space outside the first partition 13 in the casing 1 into eight fan-shaped second spaces 141 on average. A cylindrical liquid sealing tank 132 is arranged in the middle of the first space 131, the top of the liquid sealing tank 132 is open, buffer liquid is contained in the liquid sealing tank, and the opening position is sealed by a sealing film 133. A strip-shaped detection table 142 is extended in the radial direction from the middle of the second space 141, one end of the detection table 142 is connected to the first partition 13, and the other end is connected to the middle of a water absorption pad mounting portion 143 provided on the outer edge of the housing 1 in the second space 141. The pad-holding portion 143 has a rectangular shape, and has a rectangular holding space therein. The top of the detection table 142 is provided with a detection strip 3 along the direction of the detection table 142, and a first clamping part 144 for clamping and fixing the detection strip 3 is arranged in the middle of the detection table 142. The first holding portion 144 is formed of two C-shaped members oppositely disposed on both sides of the test table 142, and holds the test strip 3 by a portion of the C-shaped members higher than the test table 142. The position where the pad attaching portion 143 is connected to the test table 142 has a second holding portion 145 through which the test strip 3 passes, and a pad 4 connected to the test strip 3 is provided in the pad attaching portion 143. The third clamping portion 146 is disposed at a position of the first partition plate 13 where the detection platform 142 is connected to the first partition plate 13, and can fix the detection strip 3. The upper part of the detection strip 3 in the third clamping part 146 is also clamped and fixed with strip-shaped cushion pads 5 connected with the detection strip 3, and the eight cushion pads 5 extend towards the center of the top of the liquid sealing pool 132 and are connected with the same.

Fig. 4 is a schematic structural diagram of the detection strip 3 in fig. 3. As shown in FIG. 4, the detection strip 3 comprises a strip-shaped bottom plate 31, a nitrocellulose membrane 311 is laid on the top of the bottom plate 31, one end of the bottom plate 31 is connected with a buffer pad 5, and the other end of the bottom plate 31 is connected with a water absorbent pad 4. In the area of the first clamp portion 144 on the upper surface of the middle of the base plate 31, a rectangular parallelepiped microplate reader pad 32 having the same width as the base plate 31 is provided along the base plate 31. The top of the bottom plate 31 is positioned between the elisa pad 32 and the absorbent pad 4, and a detection line 33 close to the elisa pad 32 and a control line 34 close to the absorbent pad 4 are arranged in parallel along the width direction of the bottom plate 31.

FIG. 5 is a schematic top view of the reagent cartridge of FIG. 1. Fig. 6 is a sectional view taken along line a-a in fig. 5. As shown in fig. 5 and 6, each of the eight sample wells 11 corresponds to one microplate-labeled pad 32; eight viewing ports 12 each viewing port 12 corresponds to a set of detection lines 33 and control lines 34. The button 2 is positioned above the liquid sealing pool 132, the axial lead of the button 2 is superposed with the axial lead of the liquid sealing pool 132, meanwhile, the button 2 can be sleeved outside the liquid sealing pool 132 when moving downwards, and the spine part 22 in the button 2 can be abutted against the buffer pad 5 to enable the buffer pad 5 to damage the sealing membrane 133 and be immersed into the buffer liquid.

In addition, the enzyme label pad 32 contains an enzyme-labeled monoclonal antibody Y₁And the detection line 33 contains a monoclonal antibody Y₂Control line 34 contains a goat anti-mouse secondary antibody (or goat anti-mouse polyclonal antibody, goat anti-mouse IgG). Monoclonal antibody Y₁Binding to antigen and monoclonal antibody Y₂The formed complex will develop color; if no antigen is present, monoclonal antibody Y₁With monoclonal antibody Y₂No complex formation and no color development occurs; monoclonal antibody Y₁The complex formed with the goat anti-mouse secondary antibody in control line 34 develops color. Among them, monoclonal antibody Y in eight ELISA pads 32₁The monoclonal antibody is a CDV canine distemper virus monoclonal antibody, a CPV canine parvovirus monoclonal antibody, a CAV1 canine adenovirus 1 type monoclonal antibody, a CAV2 canine adenovirus 2 type monoclonal antibody, a CPIV canine parainfluenza virus monoclonal antibody, an RV rabies virus monoclonal antibody, a canine leptospirosis virus monoclonal antibody and a toxoplasma gondii monoclonal antibody.

When the kit is used, the kit is horizontally placed, and samples are dripped into the eight sample holes 11, so that the samples are dripped onto the enzyme labeling pad 32 through the sample holes 11. If the sample has monoclonal antibody Y in the enzyme labeling pad 32₁Corresponding antigen, which will react with monoclonal antibody Y₁And (4) combining. Pressing the button 2 downward causes the sharp portion 22 to push the cushion 5 to move downward and pierce the sealing membrane 133, so that a portion of the cushion 5 is immersed in the buffer solution reservoir 132. The buffer solution reaches the nitrocellulose membrane 311 through the buffer pad 5 under the capillary action, and reaches the enzyme labeling pad 32 along the nitrocellulose membrane 311, and after passing through the enzyme labeling pad 32, the buffer solution continues to reach the water absorption pad 4 along the nitrocellulose membrane 311 through the detection line 33 and the control line 34, so that the substances in the enzyme labeling pad 32 are transmitted to the detection line 33 and the control line 34. Goat anti-mouse secondary antibody and monoclonal antibody Y in control line 34₁The complex is formed and thus developed, and also represents that the buffer solution has passed through the detection line 33, i.e., the substance in the enzyme label pad 32 has come into contact with the detection line 33. In addition, the absorbent pad 4 has an adsorption effect on the buffer solution, so that the buffer solution can continuously flow on the nitrocellulose membrane 311 and reach the absorbent pad 4 through the enzyme labeling pad 32, the detection line 33 and the control line 34. Further, the substance in the microplate reader pad 32 can be brought into sufficient contact with the detection line 33 and the control line 34. If the sample contains antigen, the antigen in the ELISA pad 32 is mixed with the monoclonal antibody Y₁Will bind to monoclonal antibody Y in test line 33₂The binding forms a complex and develops color, which means that the sample contains the monoclonal antibody Y₁The corresponding antigen. That is, when examiningWhen the color reaction of the measuring line 33 and the control line 34 occurs simultaneously, the antigen is contained in the sample; when the detection line 33 does not develop a color reaction and the control line 34 develops a color reaction, it indicates that the sample does not contain the antigen. By the method, eight antigens in the sample can be detected simultaneously.

Second embodiment

The utility model discloses the second embodiment is still provided.

FIG. 7 is a schematic view showing the structure of the second embodiment in the axial direction of the cartridge. As shown in fig. 7, the second embodiment is different from the first embodiment in that a circular groove 15 is provided on a circle on which eight sample holes 11 are located at the top of the housing 1 in the second embodiment, and the eight sample holes 11 are located at the bottom of the groove 15. Through above-mentioned structure, when dropwise add the sample, no longer need carry out the dropwise add in sample hole 11 one by one, only need with the sample dropwise add to the groove 15 in, the sample will follow groove 15 and flow into each sample hole 11 automatically, make the detection operation more convenient, swift. In the second embodiment, the same parts as those of the first embodiment are denoted by the same reference numerals.

In the same principle, the kit of the present application can be adjusted according to the amount of the antigen to be detected. For example, a kit can be configured to detect six antigens from cats (FPV feline panleukopenia virus, FCV feline calicivirus, FHV-1 feline herpesvirus, feline rabies virus, feline Toxoplasma, feline leptospirosis virus).

The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.

Claims

1. A kit having a housing, comprising within the housing:

the liquid sealing pool is used for storing buffer liquid;

each nitrocellulose membrane is in a strip shape, and an enzyme labeling pad, a detection line and a contrast line are sequentially arranged on the upper surface of each nitrocellulose membrane;

the buffer pad, the first part is connected with the upper surface of one end of each nitrocellulose membrane, the second part is immersed in the buffer solution, the buffer solution can be conveyed to the nitrocellulose membrane through capillary action, and the buffer solution can sequentially pass through the enzyme labeling pad, the detection line and the comparison line along the nitrocellulose membrane;

wherein, different nitrocellulose membranes have monoclonal antibodies Y of different enzyme-labeled antigens in different enzyme labeling pads₁And detecting monoclonal antibody Y with corresponding antigen in line₂A goat anti-mouse secondary antibody is arranged in the control line; antigen and corresponding monoclonal antibody Y₁And monoclonal antibody Y₂A color reaction occurs, monoclonal antibody Y₁And the antibody reacts with a goat anti-mouse secondary antibody in a color reaction manner.

2. The kit according to claim 1, wherein the case has a first space and a plurality of second spaces provided around the first space; the buffer pad is arranged in the first space, the second part of the buffer pad is arranged corresponding to the first space, and the first part of the buffer pad comprises a plurality of extending parts which are arranged corresponding to the plurality of nitrocellulose membranes in an outward divergence mode.

3. The kit of claim 2, wherein the housing is cylindrical; the first space is cylindrically arranged in the middle of the shell; the plurality of second spaces are fan-shaped spaces which are evenly divided outside the first space in the shell.

4. The kit of claim 3, wherein the buffer reservoir has an opening sealed by a sealing membrane, and the other end of the buffer pad is adapted to be immersed in the buffer after the sealing membrane is broken.

5. The kit according to claim 4, wherein the case is provided with a button, and the button is provided with a spike at a position corresponding to the other end of the cushion pad, and the spike is brought into contact with the other end of the cushion pad by pressing the button to break the sealing film and then immersed in the buffer solution.

6. The kit of claim 1, wherein the housing is provided with a sample hole at a position corresponding to the elisa pad, and a viewing port at a position corresponding to the detection line and the control line.

7. A kit as claimed in claim 6, wherein the housing is provided with a well and the sample well is located at the bottom of the well.

8. The kit according to claim 1, wherein the nitrocellulose membrane is provided at the other end thereof with a water absorbent pad.