CN203537926U - Edible fungus liquid culture production and cultivation system - Google Patents
Edible fungus liquid culture production and cultivation system Download PDFInfo
- Publication number
- CN203537926U CN203537926U CN201320726309.XU CN201320726309U CN203537926U CN 203537926 U CN203537926 U CN 203537926U CN 201320726309 U CN201320726309 U CN 201320726309U CN 203537926 U CN203537926 U CN 203537926U
- Authority
- CN
- China
- Prior art keywords
- pipeline
- nutrient solution
- tank
- heat exchanger
- strain cultivation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 241000233866 Fungi Species 0.000 title claims abstract description 11
- 238000004519 manufacturing process Methods 0.000 title abstract description 5
- 238000009630 liquid culture Methods 0.000 title abstract 8
- 235000015097 nutrients Nutrition 0.000 claims abstract description 57
- 230000001954 sterilising effect Effects 0.000 claims abstract description 54
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 35
- 238000001816 cooling Methods 0.000 claims abstract description 17
- 238000012384 transportation and delivery Methods 0.000 claims abstract description 8
- 239000010865 sewage Substances 0.000 claims abstract description 5
- 239000007788 liquid Substances 0.000 claims description 35
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 24
- 235000016709 nutrition Nutrition 0.000 claims description 15
- 230000035764 nutrition Effects 0.000 claims description 15
- 230000002209 hydrophobic effect Effects 0.000 claims description 4
- 239000011148 porous material Substances 0.000 claims description 4
- 238000003756 stirring Methods 0.000 claims description 4
- 239000002826 coolant Substances 0.000 claims description 3
- 230000008676 import Effects 0.000 claims description 3
- 208000015181 infectious disease Diseases 0.000 abstract description 7
- 238000000034 method Methods 0.000 abstract description 6
- 208000037805 labour Diseases 0.000 abstract 2
- 239000000498 cooling water Substances 0.000 abstract 1
- 230000036512 infertility Effects 0.000 abstract 1
- 239000006193 liquid solution Substances 0.000 abstract 1
- 238000002255 vaccination Methods 0.000 abstract 1
- 238000004140 cleaning Methods 0.000 description 16
- 239000007789 gas Substances 0.000 description 15
- 230000001580 bacterial effect Effects 0.000 description 10
- 238000011081 inoculation Methods 0.000 description 9
- 238000010899 nucleation Methods 0.000 description 9
- 241000894006 Bacteria Species 0.000 description 3
- 230000001737 promoting effect Effects 0.000 description 3
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 239000012531 culture fluid Substances 0.000 description 2
- 230000003203 everyday effect Effects 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 238000005507 spraying Methods 0.000 description 2
- 235000004443 Ricinus communis Nutrition 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000007850 degeneration Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000029142 excretion Effects 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
Images
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Mushroom Cultivation (AREA)
Abstract
The utility model discloses an edible fungus liquid culture production and cultivation system which comprises a nutrient solution sterilization tank, a heat exchanger, a cooling-water machine set, a plurality of liquid culture cultivation tanks, a nutrient solution circulation pipeline, a nutrient solution sterilization and cooling pipeline, a nutrient solution conveying pipeline, a sterility compressed air inlet pipeline, a sewage drainage pipeline and a liquid culture delivery pipeline. The utility mode further discloses a method for producing and cultivating edible fungus liquid cultures through the system. According to the method, cultures left in the liquid culture cultivation tanks can be used as mother cultures for secondary cultivation, the infection risk for sequential vaccination is avoided, sterilization, cooling and delivery of a culture liquid solution and feeding of sterile air are accomplished in the closed containers and the pipelines, and the inflection rate is nearly zero. The liquid culture cultivation tanks do not need to be carried, investment can be reduced, the edible fungus liquid culture production and cultivation system is safe and reliable, the number of needed labors is reduced, and the labor intensity is relieved.
Description
Technical field
The utility model relates to making and the culture systems of bacterial classification, and particularly a kind of edible fungi liquid strain is made and culture systems.
Background technology
In prior art, it is all to adopt independently equipment that edible fungi liquid strain is made, and its making pattern is:
First strain cultivation tank is carried out to manual cleaning at seeding tank cleaning shop.After cleaning finishes, by manually promoting strain cultivation tank, enter strain cultivation tank weighing area.By manually the culture fluid preparing and clear water being poured in strain cultivation tank.With manually entering seeding tank sterilizer by tank promotion strain cultivation tank, carry out sterilizing again.After sterilizing finishes, by manually under hot conditions, strain cultivation tank being pulled out from seeding tank sterilizer is inner, enter seeding tank chilling room, after entering chilling room, first filtrated air pipeline is accessed to the aseptic gas interface of strain cultivation pot bottom, prevent that strain cultivation tank from forming negative pressure in cooling procedure, thereby cause in room gas to enter strain cultivation tank, cause infection.Cooling be by running water stop-spraying drench method lowered the temperature, winter, cooling need were about 8 hours.Summer the cooling 10-14 hour that needs.
After cooling end, the filtrated air pipeline being connected with the aseptic gas interface of strain cultivation pot bottom is pulled up, by manually promoting strain cultivation tank, enter hundred grades of laminar flow hood belows between seeding tank inoculation again, first filtrated air pipeline in seeding tank inoculation is accessed to the aseptic gas interface of 3 strain cultivation pot bottom, make to form malleation in tank, protect from infection.Afterwards under hundred grades of laminar flow hood by manually opening inoculation quick-actuating closure, mother is planted to access and completes inoculation in tank.
After having inoculated, first the filtrated air pipeline being connected with the aseptic gas interface of strain cultivation pot bottom is pulled up, by manually promoting strain cultivation tank, enter between strain cultivation again, after entering, by the aseptic gas interface of filtrated air pipeline access strain cultivation pot bottom between strain cultivation, to mycelia oxygen supply in tank.Cultivate one-period after 7-8 days, bacterial classification output channel is connected with body bacteria culture tank bottom bacterial classification output interface, cultured liquid spawn is flowed to inoculating facility.After inoculation finishes.Empty strain cultivation tank is manually pushed to seeding tank cleaning shop and cleans, recycle.
Mainly there is following defect in this liquid spawn making apparatus of the prior art and operator scheme:
1, working strength is large: because each bacteria culture tank need be installed castor, manually pull carrying, so gross weight can only be below one ton, or manpower is difficult to drive;
2, infection rate is high: each seeding tank needs to patch, dismantle filtrated air joint for 3 times, patches bacterial classification output channel joint for 1 time, successively opens the inoculation of inoculation quick-actuating closure in a cultivation cycle.Above process all must complete under aseptic condition, and requires employee's technical ability very skilled, otherwise very easily causes infection;
3, cost of investment is high.By every day, by 3 tanks, cultivation cycle, within 8 days, calculate.Separately add 3 non-conservation tanks, amount to 3 × 8+3=27 tank.Sterilizing need be equipped with sterilizer special;
4, water consumption is large, not stop-spraying pouring seeding tank of running water when cooling, and trickling, can not recycle in vain, causes waste.
Summary of the invention
Technical problem to be solved in the utility model is for the deficiencies in the prior art, provides a kind of reasonable in design, edible fungi liquid strain safe, that greatly reduce infection rate easy to use to make and culture systems.
Technical problem to be solved in the utility model is to realize by following technical scheme.The utility model is that a kind of edible fungi liquid strain is made and culture systems, is characterized in: this system comprises nutrient solution sterilization tank, heat exchanger, handpiece Water Chilling Units and some strain cultivation tanks; And,
Nutrient solution circulation line: its one end is connected to the top of nutrient solution sterilization tank, the other end is connected to the bottom of nutrient solution sterilization tank; This pipeline is provided with circulating pump, and this pipeline access heat exchanger picking out from heat exchanger, on Gai road, is connected with into steam pipework;
On nutrient solution sterilization tank or nutrient solution circulation line, be connected with inlet pipeline;
Nutrient solution sterilizing and cooling line: its one end is connected with the water inlet of handpiece Water Chilling Units
,the other end is connected with the delivery port of handpiece Water Chilling Units; This pipeline is provided with coolant pump, and this pipeline access heat exchanger picking out from heat exchanger, is connected with into steam pipework and hydrophobic exhaust line on Gai road;
Nutrition pipeline road: its outlet is connected to the bottom of nutrient solution sterilization tank, import is connected with the entrance of each strain cultivation tank;
Enter aseptic compressed gas pipeline: it is connected with the top of nutrient solution sterilization tank, the bottom of each strain cultivation tank respectively;
Blowoff line: its entrance point is connected with the bottom of each strain cultivation tank respectively, and discharges by sewage draining exit;
Liquid spawn transfer pipeline: its entrance point is connected with each strain cultivation pot bottom respectively, and exports by liquid spawn delivery outlet.
In system described in the utility model, further preferred technical scheme is:
1. the heat exchanger described in is heat-exchangers of the plate type;
2. on nutrient solution sterilization tank, be provided with liquid level gauge and temperature sensor;
3. in nutrient solution sterilization tank bottom, be provided with and be provided with bilge discharge pipe road;
4. on liquid spawn transfer pipeline, be connected with into steam pipework and inlet pipeline;
5. on nutrition pipeline road, be connected with inlet pipeline;
6. entering on aseptic compressed gas pipeline to be connected with pressure relief pipe;
7. in strain cultivation tank, bottom is provided with agitator;
8. the agitator described in is preferably agitator air, and described agitator air comprises stirring back shaft and establishes puddler thereon, the pore group that is each side provided with at least one group of perforate opposite direction of puddler.
In the utility model system, on each pipeline, conventional valve is set on demand or instrument is controlled.Between each pipeline, according to arranging conveniently, can adopt the mode that shares one section of pipeline to settle.
Compared with prior art, the advantage of technical solutions of the utility model is mainly reflected in:
1. the utility model system can complete independently bacterial classification making and cultivation are a kind of production of hybrid seeds equipment of integrated form.
2. the supply of the sterilizing of bacteria culture fluid, cooling, conveying and filtrated air all completes in airtight container and pipeline, and infecting probability is zero.
3. strain cultivation tank, without moving, can customize according to user's consumption every day the specification of tank body.
4. can reduce investment: do not need liquid spawn tank sterilizer.The more former scheme of strain cultivation tank quantity relatively has large batch of minimizing, but the volume of each strain cultivation tank will meet this factory's consumption of a day.
5. liquid spawn tank does not need to clean in principle, general annual cleaning 1-2 time.
6. the remaining bacterial classification in strain cultivation tank can be planted to use as the mother who again cultivates, avoid the successively infection risk of inoculation, greatly reduce and infect probability.
7. the utility model system can be controlled automatically by computer, safe and reliable, reduces the amount of labour used, has reduced labour intensity.
Accompanying drawing explanation
Fig. 1 is a kind of structural representation of the utility model system;
Fig. 2 is a kind of plan structure schematic diagram of agitator.
Embodiment
Referring to accompanying drawing, further describe concrete technical scheme of the present utility model, so that those skilled in the art understands the utility model further, and do not form the restriction to its right.
Nutrient solution circulation line 4: its one end is connected to the top of nutrient solution sterilization tank 1, the other end is connected to the bottom of nutrient solution sterilization tank 1; This pipeline is provided with circulating pump, and this pipeline access heat exchanger 10 picking out from heat exchanger, on Gai road, is connected with into steam pipework;
On nutrient solution sterilization tank 1 or nutrient solution circulation line 4, be connected with inlet pipeline;
Nutrient solution sterilizing and cooling line 2: its one end is connected with the water inlet of handpiece Water Chilling Units 3
,the other end is connected with the delivery port of handpiece Water Chilling Units 3; This pipeline is provided with coolant pump, and this pipeline access heat exchanger 10 picking out from heat exchanger, is connected with into steam pipework and hydrophobic exhaust line on Gai road;
Nutrition pipeline road 8: its outlet is connected to the bottom of nutrient solution sterilization tank 1, import is connected with the entrance of each strain cultivation tank 7;
Enter aseptic compressed gas pipeline 5: it is connected with the top of nutrient solution sterilization tank 1, the bottom of each strain cultivation tank 7 respectively;
Blowoff line 9: its entrance point is connected with the bottom of each strain cultivation tank 7 respectively, and discharges by sewage draining exit;
Liquid spawn transfer pipeline 6: its entrance point is connected with each strain cultivation tank 7 bottoms respectively, and exports by liquid spawn delivery outlet.
Use system of the present invention to carry out bacterial classification and make and the method for cultivating, its step is as follows:
(1) without bacterial nutrient solution, make:
A. add water: prepare in proportion nutrient solution, and the charge door by nutrient solution sterilization tank 1 adds in tank body by it, start on nutrient solution sterilization tank 1 or nutrient solution circulation line 4 and be connected with inlet pipeline, add running water quantitative to adjust concentration;
B. heat up: start nutrient solution circulation line 4, will in nutrition liquid pump heat exchanger 10, circulate; Nutrient solution sterilizing simultaneously and cooling line 2, the nutrient solution in heat exchanger 10 heats up; Simultaneously by hydrophobic exhaust line excretion steam condensate (SC);
C. sterilizing: when nutrient solution intensification reaches setting value, carry out the sterilizing of nutrient solution constant temperature under 100-130 degree Celsius; The amount of entering steam during intensification sterilizing is subject to temperature control, during lower than setting value, opens, and during higher than setting value, closes;
D. it is cooling: after sterilizing finishes, steam off pipeline, is pumped into the cold water of handpiece Water Chilling Units 3 in heat exchanger 10 and circulates by nutrient solution sterilizing and cooling line 2, and nutrient solution also carries out circulating cooling in heat exchanger 10 simultaneously, when solution temperature is reduced to setting value, cooling completing;
E. aseptic nutrient solution is carried: open nutrition pipeline road 8 and enter aseptic compressed gas pipeline 5, nutrient solution is delivered to respectively in strain cultivation tank 7;
F. nutrient solution sterilization tank 1 cleans: open on nutrient solution sterilization tank 1 or nutrient solution circulation line 4 and be connected with inlet pipeline, in tank, inject clear water, carry out wash cycles, heat exchanger 10 and nutrient solution sterilization tank 1 are cleaned;
(2) cultural hypha: nutrient solution enters after strain cultivation tank 7, under aseptic condition, the inoculation piece of strain cultivation tank 7 is uncapped mother is planted in access tank; During cultivation, open into aseptic compressed gas pipeline 5, aseptic gas is sent in strain cultivation tank 7, to mycelia oxygen supply;
When agitator air is housed, the puddler of the agitator air of liquid spawn transfer pipeline 6 is communicated with, and just aseptic gas evenly feeds in tank, and the pore group oppositely arranging can make puddler rotation, realizes and stirring;
(3) liquid spawn output: after cultural hypha completes, open strain cultivation tank 7, by liquid spawn transfer pipeline 6, liquid spawn is exported; After output, in tank, remaining part bacterial classification is used when female kind, while injecting new nutrient solution, need not connect new mother's kind again;
(4) strain cultivation tank 7 cleaning and sterilizings: the mother in strain cultivation tank 7 plants the degeneration of considering spawn activity after continuing to use a period of time, mother's kind that need more renew; Renew before female kind, need carry out cleaning and sterilizing to strain cultivation tank 7, its method is as follows:
A. clean: open liquid spawn transfer pipeline 6, by the emptying of residual liquid bacterial classification; By nutrition pipeline road 8, running water is sent into the strain cultivation tank 7 of required cleaning, aseptic gas is sent in tank and cleaned simultaneously, after cleaning, by blowoff line 9, sewage is discharged;
B. sterilization: after cleaning, by nutrition pipeline road 8, high-temperature steam is sent in the strain cultivation tank 7 of required sterilizing, carried out sterilizing;
(5) nutrition pipeline road 8, liquid spawn transfer pipeline 6 cleaning and sterilizings: after each conveying finishes, need carry out cleaning and sterilizing to nutrition pipeline road 8, liquid spawn transfer pipeline 6;
A. nutrition pipeline road 8 cleaning and sterilizings: running water is sent into nutrition pipeline road 8 and clean; After cleaning finishes, high-temperature steam is sent into nutrition pipeline road 8 and carry out disinfection;
B. liquid spawn transfer pipeline 6 cleaning and sterilizings: running water is sent into liquid spawn transfer pipeline 6 and clean; After cleaning finishes, high-temperature steam is sent into liquid spawn transfer pipeline 6 and carry out disinfection.
Claims (9)
1. edible fungi liquid strain is made and a culture systems, it is characterized in that: this system comprises nutrient solution sterilization tank, heat exchanger, handpiece Water Chilling Units and some strain cultivation tanks; And,
Nutrient solution circulation line: its one end is connected to the top of nutrient solution sterilization tank, the other end is connected to the bottom of nutrient solution sterilization tank; This pipeline is provided with circulating pump, and this pipeline access heat exchanger picking out from heat exchanger, on Gai road, is connected with into steam pipework;
On nutrient solution sterilization tank or nutrient solution circulation line, be connected with inlet pipeline;
Nutrient solution sterilizing and cooling line: its one end is connected with the water inlet of handpiece Water Chilling Units
,the other end is connected with the delivery port of handpiece Water Chilling Units; This pipeline is provided with coolant pump, and this pipeline access heat exchanger picking out from heat exchanger, is connected with into steam pipework and hydrophobic exhaust line on Gai road;
Nutrition pipeline road: its outlet is connected to the bottom of nutrient solution sterilization tank, import is connected with the entrance of each strain cultivation tank;
Enter aseptic compressed gas pipeline: it is connected with the top of nutrient solution sterilization tank, the bottom of each strain cultivation tank respectively;
Blowoff line: its entrance point is connected with the bottom of each strain cultivation tank respectively, and discharges by sewage draining exit;
Liquid spawn transfer pipeline: its entrance point is connected with each strain cultivation pot bottom respectively, and exports by liquid spawn delivery outlet.
2. system according to claim 1, is characterized in that: described heat exchanger is heat-exchangers of the plate type.
3. system according to claim 1, is characterized in that: on nutrient solution sterilization tank, be provided with liquid level gauge and temperature sensor.
4. system according to claim 1, is characterized in that: on nutrient solution sterilization tank bottom, be provided with bilge discharge pipe road.
5. system according to claim 1, is characterized in that: on liquid spawn transfer pipeline, be connected with into steam pipework and inlet pipeline.
6. system according to claim 1, is characterized in that: on nutrition pipeline road, be connected with inlet pipeline.
7. system according to claim 1, is characterized in that: entering on aseptic compressed gas pipeline to be connected with pressure relief pipe.
8. the system described in any one according to claim 1-7, is characterized in that: in strain cultivation tank, bottom is provided with agitator.
9. system according to claim 8, it is characterized in that: described agitator is agitator air, described agitator air comprises stirring back shaft and establishes puddler thereon, the pore group that is each side provided with at least one group of perforate opposite direction of puddler.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320726309.XU CN203537926U (en) | 2013-11-18 | 2013-11-18 | Edible fungus liquid culture production and cultivation system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320726309.XU CN203537926U (en) | 2013-11-18 | 2013-11-18 | Edible fungus liquid culture production and cultivation system |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN203537926U true CN203537926U (en) | 2014-04-16 |
Family
ID=50456117
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201320726309.XU Expired - Fee Related CN203537926U (en) | 2013-11-18 | 2013-11-18 | Edible fungus liquid culture production and cultivation system |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN203537926U (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103548577A (en) * | 2013-11-18 | 2014-02-05 | 连云港国鑫食用菌成套设备有限公司 | System and method for producing and culturing liquid spawns of edible fungus |
| CN104521563A (en) * | 2014-12-26 | 2015-04-22 | 广州苏雷锘克新能源科技有限公司 | Casing type edible fungus cultivation device |
| CN111802170A (en) * | 2019-04-10 | 2020-10-23 | 四季洋圃生物机电股份有限公司 | Environment-friendly liquid cultivation method of mushroom |
-
2013
- 2013-11-18 CN CN201320726309.XU patent/CN203537926U/en not_active Expired - Fee Related
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103548577A (en) * | 2013-11-18 | 2014-02-05 | 连云港国鑫食用菌成套设备有限公司 | System and method for producing and culturing liquid spawns of edible fungus |
| CN103548577B (en) * | 2013-11-18 | 2015-07-22 | 连云港国鑫食用菌成套设备有限公司 | System and method for producing and culturing liquid spawns of edible fungus |
| CN104521563A (en) * | 2014-12-26 | 2015-04-22 | 广州苏雷锘克新能源科技有限公司 | Casing type edible fungus cultivation device |
| CN104521563B (en) * | 2014-12-26 | 2017-02-01 | 广州苏雷锘克新能源科技有限公司 | Casing type edible fungus cultivation device |
| CN111802170A (en) * | 2019-04-10 | 2020-10-23 | 四季洋圃生物机电股份有限公司 | Environment-friendly liquid cultivation method of mushroom |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN202077430U (en) | Fermentation tank with sterilizer at outer part | |
| KR102302881B1 (en) | Microorganism automatic cultivation and distribution system | |
| CN102552896B (en) | Preparation method of porcine reproductive and respiratory syndrome vaccines by utilizing bioreactor | |
| CN203048953U (en) | Detachable plant tissue liquid culture apparatus | |
| AU2013285060A1 (en) | Photobioreactor for liquid cultures | |
| CN108249574A (en) | It is a kind of to handle pig farm breeding wastewater using microalgae and produce the device and its technique of algae powder | |
| CN203537926U (en) | Edible fungus liquid culture production and cultivation system | |
| CN101353618A (en) | Production method and fermentation device of edible fungus liquid strain | |
| CN103548577B (en) | System and method for producing and culturing liquid spawns of edible fungus | |
| CN104017721B (en) | A kind of plural serial stage fluid surface fermenting organism reactive system | |
| CN206887129U (en) | Disposable pneumo lift-type cell culture reactor | |
| CN102630493B (en) | Method and equipment for preparing liquid strain of edible and medicinal fungi by using conventional drinking water | |
| CN201878571U (en) | Dual cultivation tank of liquid spawn of edible mushroom | |
| CN109762723B (en) | Device for photosynthetic bacteria culture | |
| CN107058089A (en) | Liquid spawn fermentation tank | |
| CN202077429U (en) | Integral disinfection fermentation tank | |
| CN109251847B (en) | Apparatus and method for cultivating photosynthetic microorganisms using sunlight | |
| CN100395326C (en) | Microbial cultivation system and method for cultivating microorganisms using the system | |
| CN205347257U (en) | Bio -feritlizer fermentation system | |
| CN209957772U (en) | A device for culturing photosynthetic bacteria | |
| CN2675680Y (en) | A liquid strain production tank | |
| CN216764869U (en) | Tertiary fermenting installation of benefit fungus | |
| CN105325171A (en) | Integrated sterilization fermentation cylinder | |
| CN208250300U (en) | A kind of ferment strain three-level continuously spreads cultivation device | |
| CN102416186B (en) | Regular disinfection method for continuous unicellular alga culture system and vapor disinfection system thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20140416 Termination date: 20161118 |