CN203432973U - Carbon nano-tube micro-cantilever biosensor for detecting tumor markers - Google Patents
Carbon nano-tube micro-cantilever biosensor for detecting tumor markers Download PDFInfo
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- CN203432973U CN203432973U CN201320374839.2U CN201320374839U CN203432973U CN 203432973 U CN203432973 U CN 203432973U CN 201320374839 U CN201320374839 U CN 201320374839U CN 203432973 U CN203432973 U CN 203432973U
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- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 title claims abstract description 48
- 239000002041 carbon nanotube Substances 0.000 title claims abstract description 44
- 229910021393 carbon nanotube Inorganic materials 0.000 title claims abstract description 44
- 206010028980 Neoplasm Diseases 0.000 title abstract description 24
- 239000000463 material Substances 0.000 claims abstract description 22
- 238000001514 detection method Methods 0.000 claims abstract description 16
- 239000000439 tumor marker Substances 0.000 claims abstract description 4
- 108091008104 nucleic acid aptamers Proteins 0.000 claims abstract 2
- 238000000034 method Methods 0.000 claims description 10
- 230000000694 effects Effects 0.000 claims description 6
- 229910052710 silicon Inorganic materials 0.000 claims description 4
- 239000010703 silicon Substances 0.000 claims description 4
- 238000000576 coating method Methods 0.000 claims description 3
- 238000000197 pyrolysis Methods 0.000 claims 1
- 108091023037 Aptamer Proteins 0.000 abstract description 16
- 238000005516 engineering process Methods 0.000 abstract description 3
- 150000001875 compounds Chemical class 0.000 description 9
- 239000000523 sample Substances 0.000 description 7
- 230000004048 modification Effects 0.000 description 5
- 238000012986 modification Methods 0.000 description 5
- 238000006243 chemical reaction Methods 0.000 description 3
- 230000001419 dependent effect Effects 0.000 description 3
- 230000002209 hydrophobic effect Effects 0.000 description 3
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 2
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 description 2
- 239000011248 coating agent Substances 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000006073 displacement reaction Methods 0.000 description 2
- 238000004518 low pressure chemical vapour deposition Methods 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000005253 cladding Methods 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 239000008367 deionised water Substances 0.000 description 1
- 229910021641 deionized water Inorganic materials 0.000 description 1
- 230000001934 delay Effects 0.000 description 1
- 238000007306 functionalization reaction Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000004377 microelectronic Methods 0.000 description 1
- -1 pick-up circuit Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 239000000377 silicon dioxide Substances 0.000 description 1
- 239000002210 silicon-based material Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 238000004506 ultrasonic cleaning Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
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Abstract
用于肿瘤标志物检测的碳纳米管微悬臂梁生物传感器，包括包括支架(1)，基底材料(2)，碳纳米管(3)、拾取电路(4)；基底材料(2)固定在支架(1)一侧构成微悬臂梁结构，碳纳米管(3)生长在基底材料(2)的上面，拾取电路(4)在基底材料(2)的下面；还包括附在碳纳米管(3)上面的核酸适配体(5)。本实用新型利用修饰在碳纳米管上的核酸适配体检测肿瘤标志物。以微悬臂梁作为肿瘤标志物检测的传感器平台，易于实现检测的高通量、微型化、阵列化要求，实现肿瘤标志物多种指标联合检测的目的。微悬臂梁通过MEMS加工工艺制成，可进行批量生产，从而降低器件的成本。
A carbon nanotube microcantilever biosensor for tumor marker detection, including a bracket (1), a base material (2), carbon nanotubes (3), and a pick-up circuit (4); the base material (2) is fixed on the bracket (1) One side forms a micro-cantilever beam structure, carbon nanotubes (3) are grown on the base material (2), and the pick-up circuit (4) is under the base material (2); it also includes carbon nanotubes (3) ) above the aptamer (5). The utility model uses nucleic acid aptamers modified on carbon nanotubes to detect tumor markers. Using the micro-cantilever beam as the sensor platform for tumor marker detection can easily meet the requirements of high-throughput, miniaturization, and arraying for detection, and achieve the purpose of joint detection of multiple indicators of tumor markers. The micro-cantilever beam is made by MEMS processing technology and can be mass-produced, thereby reducing the cost of the device.
Description
Technical field
The present invention relates to biomedical engineering field, relate in particular to a kind of carbon nano-tube micro-cantilever biology sensor.
Background technology
The method of utilizing sensor to detect tumor markers has been reported, and has quality and heat immunosensor, electrochemical immunosensor etc.These sensors are general, and only for the detection of single tumor markers, false positive rate is higher, detects degree of accuracy inadequate, easily delays or increases the weight of the state of an illness.Develop new detection technique, improve positive rate and accuracy of detection, realize online and detecting, become technical matters urgently to be resolved hurrily.
Micro-cantilever beam sensor is fixed together determinand and micro-cantilever by certain mode, by the deflection displacement of micro-cantilever or the variation of resonance frequency, realize signal conversion.Name is called the method for utilizing the micro-cantilever beam sensor that functionalized carbon nano-tube is sensitive material, and the application for a patent for invention that publication number is CN201010241824.X, is combined carbon nano-tube with micro-cantilever, realizes the detection to specific gas.The method is highly sensitive, reproducible; Weak point is to carbon nano-tube, to carry out functionalization base group modification according to the chemical property of object gas, and operation is comparatively complicated.The sensor platform that the micro-cantilever of usining detects as tumor markers, utilize the specific reaction between aptamer and tumor markers to form compound, this compound produces mass effect on carbon nano-tube micro-cantilever biology sensor, and the technology of utilizing this mass effect to realize tumor markers detection have not been reported.
Summary of the invention
Technical matters to be solved by this invention is to provide a kind of carbon nano-tube micro-cantilever biology sensor detecting for tumor markers, and this biology sensor size is little, simple in structure, easy to operate, can realize online many indexs detects.
In order to solve this technical problem, the present invention realizes the detection to tumor markers by modification of nucleic acids aptamers in carbon nano-tube.
Biology sensor of the present invention comprises the aptamer in support, base material, pick-up circuit, carbon nano-tube, carbon nano-tube.Described base material is fixed on support one side and forms micro cantilever structure, the preferred silicon of base material; Described pick-up circuit part is below base material; Described pick-up circuit part connects into wheatstone bridge form by four voltage dependent resistor (VDR)s; Described carbon nano tube growth forms carbon nano-tube micro-cantilever beam sensor on micro-cantilever; Described aptamer is to be modified in carbon nano-tube by hydrophobic effect, can be to be also modified in carbon nano-tube by π-π superposition; Aptamer is reacted and is formed compound by specific recognition with tumor markers, and the mass effect of utilizing this compound to produce on micro-cantilever biology sensor realizes detection.
The sensor platform that the present invention utilizes micro-cantilever to detect as tumor markers, is made into a kind of carbon nano-tube micro-cantilever biology sensor that can realize Diagnostic Value of Several Serum Tumor Markers detection.
The features and advantages of the invention:
1, built a kind of carbon nano-tube micro-cantilever biology sensor that detects tumor markers.This sensor, by modification of nucleic acids aptamers in carbon nano-tube, utilizes aptamer to react with tumor markers and forms compound, and this compound produces mass effect on micro-cantilever, utilizes this mass effect to realize the detection to tumor markers.
2, because growth on micro-cantilever has carbon nano-tube, modification of nucleic acids aptamers in carbon nano-tube, so this biology sensor size is little, simple in structure; The design is new reasonable, easy to operate, can realize online detection.
Accompanying drawing explanation
Fig. 1 is the schematic diagram that tumor markers detects the carbon nano-tube micro-cantilever biology sensor of use.
Embodiment
Below in conjunction with drawings and Examples, the present invention is described in further detail, but is not limited to this embodiment.
Embodiment:
Referring to Fig. 1, Fig. 1 is the schematic diagram that tumor markers detects carbon nano-tube micro-cantilever biology sensor, comprises support 1, base material 2, carbon nano-tube 3, pick-up circuit 4 and be attached to carbon nano-tube 3 aptamer 5 above.Wherein base material 2 is fixed on support 1 one sides formation micro cantilever structures; Carbon nano-tube 3 be grown in base material 2 above; And pick-up circuit 4 is below base material 2; On carbon nano-tube 3, by hydrophobic effect, be modified with one deck aptamer 5.
The present invention is according to following common process preparation and operation
1, the manufacture of micro cantilever structure
Micro-cantilever is to be base material 2 by Semiconducting Silicon Materials, is processed into micro cantilever structure.
2, the making of pick-up circuit 4
Pick-up circuit is to utilize microelectronic technique to make silicon voltage dependent resistor (VDR) at base material 2 lower surfaces, and four voltage dependent resistor (VDR)s are connected into wheatstone bridge form.
3, semi-girder growth and coating carbon nanotube technique
Upper surface to the base material 2 in abovementioned steps carries out cleaning treatment, carries out Ultrasonic Cleaning respectively with acetone, absolute ethyl alcohol, deionized water, then uses Low Pressure Chemical Vapor Deposition (LPCVD) carbon nano-tube.Also can use pyrolysismethod, cladding process or additive method carbon nano-tube coating on silica-based.
4, the modification of aptamer on carbon nano-tube micro-cantilever
Aptamer is modified in carbon nano-tube by hydrophobic effect, forms a kind of detector probe of energy specific recognition tumor markers, thereby built carbon nano-tube micro-cantilever biology sensor; Aptamer can be to be also modified in carbon nano-tube by π-π superposition.
The step that the present invention detects tumor markers is as follows:
(1) on carbon nano-tube micro-cantilever, first make the detector probe that contains tumor markers aptamer;
(2) detector probe is put into sample to be tested, in sample to be tested, tumor markers is formed compound and is attached on micro-cantilever by the aptamer in specific reaction and detector probe.
(3) in the quality of formed compound size and sample to be tested, tumor-marker substrate concentration is proportionate.
(4) mass change that described compound produces on micro-cantilever causes the variation of micro-cantilever deflection displacement or resonance frequency, thereby realizes the detection to tumor markers.
Claims (4)
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| Application Number | Priority Date | Filing Date | Title |
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| CN201320374839.2U CN203432973U (en) | 2013-06-27 | 2013-06-27 | Carbon nano-tube micro-cantilever biosensor for detecting tumor markers |
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| CN201320374839.2U CN203432973U (en) | 2013-06-27 | 2013-06-27 | Carbon nano-tube micro-cantilever biosensor for detecting tumor markers |
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| CN203432973U true CN203432973U (en) | 2014-02-12 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103293309A (en) * | 2013-06-27 | 2013-09-11 | 桂林电子科技大学 | Carbon nano-tube micro-cantilever biosensor for detecting tumor markers |
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- 2013-06-27 CN CN201320374839.2U patent/CN203432973U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103293309A (en) * | 2013-06-27 | 2013-09-11 | 桂林电子科技大学 | Carbon nano-tube micro-cantilever biosensor for detecting tumor markers |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20140212 Termination date: 20150627 |
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| EXPY | Termination of patent right or utility model |