CN203310752U - Aflatoxin quantitative detector - Google Patents

Abstract

The utility model relates to a sample liquid antigen detection device, in particular to an aflatoxin quantitative detector, which belongs to the technical field of sample liquid quantitative detection. A quantitative aflatoxin detector, characterized in that it includes aflatoxin colloidal gold immunochromatographic test paper (5), a light-emitting diode (1), uniform light glass (4), a filter (3), and a photosensitive sensor (2), the light emitted by the light-emitting diode (1) is transmitted through the homogenizing glass to the color-developing area of the aflatoxin colloidal gold immunochromatography test paper (5), and the reflected fluorescence is transmitted through the homogenizing glass ( 4) Pass through the filter (3) onto the photosensitive sensor (2); the photosensitive sensor (2) is connected with the control system. The aflatoxin quantitative detector of the utility model can not only perform qualitative interpretation but also quantitatively detect the content of aflatoxin in samples.

Description

A kind of aflatoxin quantitative detector

technical field

The utility model relates to a sample liquid antigen detection device, in particular to an aflatoxin quantitative detector, which belongs to the technical field of sample liquid quantitative detection. the

Background technique

Colloidal gold immunochromatography test paper is based on rapid diagnostic technology, see Figure 1, the principle is to fix the specific antibody on a certain zone of the nitrocellulose membrane first, when one end of the dry nitrocellulose membrane is immersed in the sample, due to the capillary function, the sample will move forward along the membrane, and when it moves to the area where the antibody is immobilized, the corresponding antigen in the sample will specifically bind to the antibody, and the area can be displayed if stained with immunocolloidal gold or immunoenzyme A certain color, so as to achieve specific immunodiagnosis. the

This experimental method can qualitatively detect whether there is a corresponding antigen in the sample, that is, a certain value of positive and negative judgments, and quantitative detection cannot be completed; even if the degree of discoloration can roughly determine the content of the corresponding antigen in the sample liquid , however, this quantitative determination is rough and imprecise, often unable to achieve the required detection accuracy. the

In the prior art, for the detection of aflatoxin, there are also thin-layer chromatography for measurement. After the aflatoxin in the sample is extracted, concentrated, and thin-layer separated, it will reflect blue-purple fluorescence through light irradiation. According to its The blue-purple fluorescence emitted on the thin layer is used to qualitatively determine the negative and positive values of a certain value. This method is suitable for Aspergillus flavus in various foods such as grains, peanuts and their products, potatoes, beans, fermented foods, and wines. Determination of prime B1. After extraction, concentration, and thin-layer separation of aflatoxin B1 in the sample, it produces blue-violet fluorescence under light with a wavelength of 365nm, and its content is determined according to the minimum detection amount of fluorescence displayed on the thin layer. Its disadvantages are: complex operation: professionals need to use multiple supporting instruments in a specialized laboratory for several hours to complete the detection. In addition, certain toxic organic solvents are used in the detection process. the

Contents of the invention

In order to solve the technical problem of the appeal, the utility model provides a quantitative detector of aflatoxin. Quantitative detection of aflatoxin by measuring the reflection of the color-developed area of the aflatoxin colloidal gold immunochromatographic test paper to light of a specific wavelength. When in use, the keyboard inputs measurement commands, irradiates the colloidal gold immunochromatographic test paper and receives optical feedback signals, the micro-processing system calculates the results, and the display shows the results. the

The utility model adopts the following technical solutions:

A kind of aflatoxin quantitative detector, including aflatoxin colloidal gold immunochromatographic test paper 5, light-emitting diode (LED) 1, homogeneous glass 4, filter 3, photosensitive sensor 2, the light emitted by the light-emitting diode 1 The light is transmitted to the color region of the aflatoxin colloidal gold immunochromatographic test paper 5 through the uniform glass, and the reflected fluorescence passes through the filter lens 3 through the uniform glass 4 to the photosensitive sensor 2; the photosensitive sensor 2 is connected with the control system. the

Further, the photosensitive sensor is CMOS (Complementary Metal Oxide Semiconductor) integration. the

Further, the wavelength of the light emitted by the light emitting diode is 660nm. the

Further, the control system includes a micro-processing system, and the micro-processing system is respectively connected with a parameter memory, a keyboard, a communication interface, an LCD display, and a USB interface, and the USB interface is connected with a host computer. the

The utility model is characterized in that: after the light of a specific wavelength is irradiated on the color region 501 of the aflatoxin colloidal gold immunochromatography test paper 5, it can reflect a specific amount of fluorescence, that is, the reflected fluorescence intensity is consistent with the color region 501. The amount of aflatoxin above has a specific corresponding relationship. Utilizing this feature, a quantitative aflatoxin detector is designed and provided. The quantitative aflatoxin detector can not only obtain the reflected fluorescent signal, collect corresponding data, and process the detected aflatoxin through the control system. The results are reflected on the display. the

Quantitatively detect aflatoxin by measuring the reflection of the color-developed area of the aflatoxin colloidal gold immunochromatographic test paper to light of a specific wavelength. When in use, the keyboard inputs measurement commands, irradiates the colloidal gold immunochromatographic test paper and receives optical feedback signals, the micro-processing system calculates the results, and the display shows the results. the

The beneficial effects of the utility model are:

1) Fast: the entire testing process including instrument testing only takes 20 minutes;

2) Inexpensive: Aflatoxin B1 colloidal gold immunochromatographic test strips are low in cost. the

3) Good operability: dry chemical method reagents are matched with instruments, and the operation is simple. the

4) Quantitative determination: not only qualitative interpretation but also quantitative detection of the content of aflatoxin B1 in the sample. the

Description of drawings

Fig. 1 is a schematic diagram of the structure of a colloidal gold immunochromatographic test paper. the

Fig. 2 is a schematic diagram of the utility model aflatoxin quantitative detector irradiating the colloidal gold immunochromatography test paper and receiving the optical feedback signal. the

Fig. 3 is a schematic diagram of the data processing flow. the

Among them, 1. light-emitting diode, 2. photosensitive sensor, 3. filter, 4. uniform light glass, 5. aflatoxin colloidal gold immunochromatographic test paper, 501. color developing area. the

Detailed ways

Below in conjunction with specific embodiment the utility model is further described. the

Referring to Figure 1, the sample liquid is dropped on the sample pad of the aflatoxin colloidal gold immunochromatographic test paper, chromatographically to the right, and reaches T and C, that is, the color developing area 501 in Figure 2;

See Fig. 2-Fig. 3, keyboard input measurement command, two 660-wavelength LEDs uniformly irradiate the color area 501 of the colloidal gold immunochromatography test paper through the uniform glass 4, and pass through the filter 3 by CMOS (complementary metal oxide semiconductor) The camera receives the feedback light signal. The received optical signal enters the microprocessor system through the communication interface for calculation, and the final result is displayed on the LCD display. The USB interface is responsible for communicating with the host computer to control parameters. The parameter memory is responsible for storing detection data and control parameters. The principle of its quantification is that, using the host computer input, the parameter memory stores the standard curve, and characterizes the functional relationship between the feedback optical signal and the content of aflatoxin B1 in the detection sample. The detector compares the optical signal with the standard curve to obtain the Aflatoxin B1 content. the

The specific operation steps are as follows:

(1) Instrument calibration: Put the uncolored aflatoxin B1 colloidal gold immunochromatography test paper into the instrument, find out the correction factor of the instrument and calibrate it. the

(2) Preparation of aflatoxin B1 standard curve: Weigh 1ug/kg to 100ug/kg aflatoxin B1 standard with precise gradient, measure it on aflatoxin quantitative detector and make a standard curve, pass the upper Machine into the aflatoxin quantitative detector. the

(3) Determination of the concentration of the test sample: Take 50ul of the test sample and drop it into the aflatoxin B1 colloidal gold immunochromatography test paper, put it into the aflatoxin quantitative detector after 10 minutes, measure, and the instrument will get the test sample concentration and displayed on the display. the

Claims (4)

1. A kind of aflatoxin quantitative detector, is characterized in that: Including aflatoxin colloidal gold immunochromatographic test paper (5), light-emitting diode (1), uniform light glass (4), filter (3), photosensitive sensor (2), the light emitted by the light-emitting diode (1) It is transmitted through the homogenizing glass to the color-developing area of the aflatoxin colloidal gold immunochromatographic test paper (5), and the reflected fluorescence passes through the homogenizing glass (4) and passes through the filter (3) to the photosensitive sensor (2 )superior; The photosensitive sensor (2) is connected with the control system. the 2. Aflatoxin quantitative detector as claimed in claim 1, characterized in that: said photosensitive sensor is CMOS integration. the 3. The aflatoxin quantitative detector as claimed in claim 1, characterized in that: the wavelength of the light emitted by the light-emitting diode is 660nm. the 4. aflatoxin quantitative detector as claimed in claim 1, is characterized in that: described control system comprises microprocessing system, and described microprocessing system is connected with parameter memory, keyboard, communication interface, LCD display, USB interface respectively. Connect, the USB interface is connected with the upper computer. the

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