CN203203853U

CN203203853U - Liquid based thin-layer cell free settling and flaking device

Info

Publication number: CN203203853U
Application number: CN201320049475.0U
Authority: CN
Inventors: 陈秀英; 杨熙
Original assignee: CIXI SANYANG MEDICAL TECHNOLOGY Co Ltd
Current assignee: CIXI SANYANG MEDICAL TECHNOLOGY Co Ltd
Priority date: 2013-01-30
Filing date: 2013-01-30
Publication date: 2013-09-18
Anticipated expiration: 2023-01-30

Abstract

The utility model discloses a liquid based thin-layer cell free settling and flaking device which comprises a cell preserving fluid bottle, a settling cabin connector, a glass slide and a settling cabin support, wherein the glass slide can be freely picked or placed in the settling cabin support, the settling cabin connector is provided with a channel through which cell sap passes, the first end of the settling cabin connector can be connected to a bottle opening of the cell preserving fluid bottle in a sealing manner, and the second end of the settling cabin connector can be pressed on the glass slide in a sealing manner and connected with the settling cabin support into a whole. Specimens of cell free settling are manufactured in an all-closed environment, and the liquid based thin-layer cell free settling and flaking device has the advantages that firstly, the cell specimens are protected from being polluted in a flaking process, the environment is not polluted, a human body is not hurt; secondly, remained cell specimens can be completely recycled so as to be used for re-inspection and other virus detection; fourthly, an external force is not applied, the cell specimens are always kept in an original state without distortion, multiple cell specimens exist on the glass slide, and are uniformly spread, an accurate and reliable basis is provided for medical detection and diagnosis; the simplicity is achieved in flaking operation, less procedures are needed, the efficiency is high, the energy is saved and the consumption is reduced.

Description

Liquid based thin-layer cell natural subsidence pelletizer

Technical field

The utility model relates to the cell specimen pelletizer, relates in particular to a kind of liquid based thin-layer cell natural subsidence pelletizer.

Background technology

The liquid based thin-layer cell detects (TCT) technology and introduces China from last century from the America and Europe, and the medical science that now has been widely used in tumor prevention inspection and examination detects.The TCT that generally uses detects the film-making mode at present three kinds: (1), membrane type film-making are (by utilizing negative pressure, be adsorbed on the filtering net film that the aperture is 5-8um collecting the cell specimen liquid of preserving in the liquid bottle, with positive air pressure the cell specimen on the filtering membrane blown to be attached to then and carry on the sloping sheet).(2), centrifugal film-making (will be collected and preserve cell specimen liquid in the liquid bottle, concentrate at the bottom of preservation liquid bottle, after the part supernatant is removed in suction with the centrifugal cell that makes of hydro-extractor, the mixing remainder, should mix liquid and inject a centrifugal film-making frame, and use hydro-extractor centrifugal again, and cell specimen is got rid of be attached on the microslide.(3), the automatic sedimentation film-making (relies on hydro-extractor will preserve the bottle end that the cell specimen in the liquid bottle concentrates, after whole supernatants are removed in suction, adds a certain amount of damping fluid and mixing.Should mix liquid again and inject a film-making frame with the powerful back of stirring of machine, be attached on the microslide through cell after the sedimentation).There is following technological deficiency in above film-making mode:

1, cell specimen must be proposed to finish film-making outside the bottle, therefore, cell specimen is vulnerable to the cross pollution between cell specimen in environmental pollution and the film-making process, makes the film-making distortion.

2, propose to preserve the outer film-making of liquid bottle after, do not attach to that remaining cell specimen can not reclaim on the microslide, can only abandon to external environment virulent material contaminated environment and influence health very easily in the sample.

3, must use external force packed cells sample, or by force cell specimen be attached on the microslide, the stressed back of cell specimen form produces distortion in various degree, influences the accuracy that medical science is read the sheet diagnosis.

The utility model content

Technical problem to be solved in the utility model is at above-mentioned prior art present situation, provide a kind of can be in enclosed environment film-making and the cell specimen print quality that makes can meet the required liquid based thin-layer cell natural subsidence pelletizer of medical diagnosis.

The utility model solves the problems of the technologies described above the technical scheme that adopts: this liquid based thin-layer cell natural subsidence pelletizer, comprise cell-preservation liquid bottle and microslide, it is characterized in that: also include a sedimentation cabin bearing and sedimentation cabin interface, described microslide can freely pick and place in the bearing of sedimentation cabin, described sedimentation cabin interface has the passage that passes through for cell liquid, first end of sedimentation cabin interface can be sealedly connected on the bottleneck of described cell-preservation liquid bottle, and second end can seal to be pressed on the described microslide and with described sedimentation cabin bearing and connect as one.

Can adopt the multiple mode that is tightly connected between sedimentation cabin interface and the cell-preservation liquid bottle, preferably, adopt between first end of described sedimentation cabin interface and the bottleneck of cell-preservation liquid bottle to be spirally connected or clamping.

In order to prevent that when making cell specimen the cell in the cell-preservation liquid bottle from leaking, second end of described sedimentation cabin interface is provided with O-ring seal.

Between sedimentation cabin interface and the sedimentation cabin bearing multiple syndeton can be arranged, preferably, described sedimentation cabin interface card is connected on the bearing of sedimentation cabin.

Further preferred as such scheme, it is box-like with the suitable strip of microslide that described sedimentation cabin bearing is, described microslide is located at the bottom of sedimentation cabin bearing, all form opening at the top of sedimentation cabin bearing and a side of length direction, on the two side of length direction, respectively form an evagination and with the suitable arcwall of second end of sedimentation cabin interface, be respectively equipped with a horizontal bayonet socket at described arcwall, be provided with two fixture blocks radially at second end of sedimentation cabin interface, described radially fixture block snaps in the corresponding horizontal bayonet socket and sedimentation cabin interface card is connected on the bearing of sedimentation cabin.

Compared with prior art, the utility model is not owing to adopt equipment and external force to get involved to collecting the cell specimen liquid of preserving in the liquid bottle, do not need to propose to preserve cell specimen outside the liquid bottle, the preparation of specimen that under a totally-enclosed environment, finishes the cell natural subsidence, thereby has a following advantage: (1), definitely guarantee that cell specimen is not contaminated in the film-making process, also free from environmental pollution and harmful to human.(2), can all reclaim the remaining cell sample, to be used for reinspection and other virus detection.(3), do not impose external force, it is undistorted that cell specimen remains ortho states, and because of natural subsidence, cell specimen quantity on microslide is many, and evenly tiling provides foundation accurately and reliably for medical science detects with diagnosis.(4), whole film-making is simple to operate, operation is few, efficient, energy-saving consumption-reducing.

Description of drawings

Fig. 1 is the structural representation of the utility model embodiment when making cell specimen;

Fig. 2 is the perspective exploded view of the utility model embodiment.

Embodiment

Describe in further detail below in conjunction with the utility model of accompanying drawing embodiment.

As depicted in figs. 1 and 2, the liquid based thin-layer cell natural subsidence pelletizer in the present embodiment comprises cell-preservation liquid bottle 1, sedimentation cabin interface 2, microslide 3 and sedimentation cabin bearing 4.Wherein, sedimentation cabin interface 2 has vertical passage to be passed through for cell liquid, first end of sedimentation cabin interface 2 is rounded and be sealedly connected on the bottleneck of cell-preservation liquid bottle 1, in the present embodiment, first end of sedimentation cabin interface 2 has screw socket 22 with formation screw socket end, and is threaded with the bottleneck employing of cell-preservation liquid bottle 1; Second end of sedimentation cabin interface 2 is also rounded and be provided with O-ring seal 5, has two radially fixture blocks 21 that distribute relatively to form the bayonet socket end at second end of sedimentation cabin interface 2.Sedimentation cabin bearing 4 is elongated box-like, microslide 3 can be placed on the bottom of sedimentation cabin bearing 4, the top of sedimentation cabin bearing 4 and a side of length direction all form opening, on the two side 41 of length direction, respectively form an evagination and with the suitable arcwall 42 of the bayonet socket end of sedimentation cabin interface 2, on arcwall 42, form the horizontal bayonet socket 43 that matches with fixture block 21 radially respectively.

During film-making, adopt following operation steps:

1), microslide 3 is put into sedimentation cabin bearing 4, again the bayonet socket end of sedimentation cabin interface 2 is pushed down microslide 3 surfaces, and rotation sedimentation cabin interface 2 makes radially, and fixture block 21 is stuck in the horizontal bayonet socket 43, at this moment, sedimentation cabin interface 2 blocks microslide 3 and makes sedimentation cabin interface 2, microslide 3 and sedimentation cabin bearing 4 these sedimentation cabins of three parts formation.

2), the bottle cap of cell-preservation liquid bottle 1 is backed out, at this moment, in cell-preservation liquid bottle 1, fill cell specimen liquid and preserve the mixed liquid that liquid forms, the screw socket end of sedimentation cabin interface 2 is screwed on the bottleneck of cell-preservation liquid bottle 1, form a totally enclosed cell settlement cabin device, guarantee that cell specimen and the mixed liquid of preserving liquid formation can enter the sedimentation cabin smoothly, do not leak.

The cell settlement cabin device that 3), will seal shakes up the back and is inverted (cell-preservation liquid bottle bottleneck down), cell specimen in the cell-preservation liquid bottle 1 with preserve the mixed liquid that liquid forms, flow to the sedimentation cabin and be contained on microslide 3 planes by the passages in the sedimentation cabin interface 2.At this moment, be suspended in the cell specimen that mixes in the liquid because of the self gravitation natural subsidence to microslide 3, and the microslide 3 that is had positive ion polarity adsorbs.

4), after settling time of setting, the cell settlement cabin device of this sealing is just placed (the cell-preservation liquid bottleneck up), the cell specimen that is not adsorbed in the cell-preservation liquid bottle 1 and all the other are preserved liquid and are mixed liquid and all be recovered to automatically in the cell-preservation liquid bottle 1.

5), screw off sedimentation cabin and sedimentation cabin interface 2 successively, take out microslide 3, the cell specimen (normally neck tube cell and " macronucleus " cell) that is deposited in advance on the microslide 3 just is adsorbed onto on the microslide 3, be at microslide 3 and show one deck uniform cell specimen that tiles, cell specimen print dyeing back is used for diagnosis.It should be noted that to preventing in the cell-preservation liquid bottle 1 cell specimen liquid and preserving liquid and polluted, after screwing off the sedimentation cabin, should be in time the bottle cap of cell-preservation liquid bottle 1 be covered.

The above only is preferred implementation of the present utility model; should be pointed out that for those of ordinary skills, do not breaking away under the principle prerequisite of the present utility model; can make multiple remodeling or improvement to the utility model, these all are regarded as within the protection domain of the present utility model.

Claims

1. liquid based thin-layer cell natural subsidence pelletizer, comprise cell-preservation liquid bottle (1) and microslide (3), it is characterized in that: also include a sedimentation cabin bearing (4) and sedimentation cabin interface (2), described microslide (3) can freely pick and place in sedimentation cabin bearing (4), described sedimentation cabin interface (2) has the passage that passes through for cell liquid, first end of sedimentation cabin interface (2) can be sealedly connected on the bottleneck of described cell-preservation liquid bottle (1), and second end can seal to be pressed on the described microslide (3) and with described sedimentation cabin bearing (4) and connect as one.

2. liquid based thin-layer cell natural subsidence pelletizer according to claim 1 is characterized in that: adopt between the bottleneck of first end of described sedimentation cabin interface (2) and cell-preservation liquid bottle (1) to be spirally connected or clamping.

3. liquid based thin-layer cell natural subsidence pelletizer according to claim 1 and 2, it is characterized in that: second end of described sedimentation cabin interface (2) is provided with O-ring seal (5).

4. liquid based thin-layer cell natural subsidence pelletizer according to claim 1 and 2, it is characterized in that: described sedimentation cabin interface (2) is connected on the sedimentation cabin bearing (4).

5. liquid based thin-layer cell natural subsidence pelletizer according to claim 4, it is characterized in that: described sedimentation cabin bearing (4) is box-like with the suitable strip of microslide (3), described microslide (3) is located at the bottom of sedimentation cabin bearing (4), all form opening at the top of sedimentation cabin bearing (4) and a side of length direction, on the two side (41) of length direction, respectively form an evagination and with the suitable arcwall (42) of second end of sedimentation cabin interface (2), be respectively equipped with a horizontal bayonet socket (43) at described arcwall (42), be provided with two fixture blocks (21) radially at second end of sedimentation cabin interface (2), described radially fixture block (21) snaps in the corresponding horizontal bayonet socket (43) and sedimentation cabin interface (2) is connected on the sedimentation cabin bearing (4).