CN202748529U - Objective table capable of automatically rotating, positioning and culturing - Google Patents

Abstract

An object stage for automatic rotation positioning and cultivation, comprising an object stage shell, on which the stage is installed, and a rotating motor is arranged in the middle of the object stage to control the stage on the stage The object stage shell is rotated, and a stepping motor is provided on the adjacent two sides of the object stage shell, and two lead screws are respectively matched with the stepping motors on the two sides to control the object stage. It moves forward, backward, left, and right on the shell of the stage, and the stage is provided with a plurality of sample positions, and the center of each sample position is a light-transmitting hole. The object table is circular, and a plurality of the sample positions are evenly arranged on the circumference of the object table. The sample position is a small circular stage with a diameter of 3.5 cm. A temperature control module is arranged below the object table. The utility model greatly improves the observation and detection efficiency of medical or biological samples, uses the computer to record the position and imaging, and greatly facilitates the operation of selecting a specific sample in the field of view and the accuracy of experiments.

Description

Auto-rotating stage for positioning and culturing

technical field

The utility model relates to an object platform for automatic rotation, positioning and cultivation, in particular to an object platform for automatic rotation, positioning and cultivation for a biological microscope.

Background technique

At present, the mobile stage of the automatic biological microscope can move along the X and Y directions according to the specified step distance according to the instructions issued by the computer or the control box, and reach the specified position, realizing the automatic control of the 2 axes of the biological microscope, and can be used as a separate Accessories are installed on various types of ordinary biological microscopes, but this type of stage can only realize the movement, positioning and observation of a single culture plate, culture bottle or culture dish, etc., and cannot realize multiple culture plates, culture bottles or culture dishes. Real-time observation of dishes.

Contents of the invention

The problem to be solved by the utility model is to provide an object stage that can be automatically rotated, positioned and cultivated for a biological microscope, so as to realize automatic observation and positioning of samples in multiple culture vessels.

For this reason, a kind of object stage of automatic rotation positioning and cultivation of the present utility model comprises an object stage shell, and the object stage is installed on the object stage shell, and a rotating motor is arranged in the middle of the object stage, and the control The stage rotates on the stage shell, and a stepping motor is provided on the adjacent two sides of the stage shell, and two lead screws are respectively matched with the stepping motors on the two sides, To control the stage to move back and forth, left, and right on the shell of the stage, the stage is provided with a plurality of sample positions, and the center of each sample position is a light-transmitting hole.

Wherein, the object table is circular, and a plurality of the sample positions are evenly arranged on the circumference of the object table.

Wherein, the sample position is a small circular stage with a diameter of 3.5 cm.

Wherein, a temperature control module is provided below the stage.

Wherein, the temperature control module maintains a preset constant temperature within the range of -20°C to +90°C.

Wherein, a rotating disk cover matching the object table is covered above the object table.

Wherein, the stepping motor, the rotating motor and the temperature control module are all connected with a computer or a control box.

The utility model greatly improves the observation and detection efficiency of medical or biological samples. By using the automatic movement and focusing of the stage, objects in different fields of view and different levels in each detection sample can be observed, and the position and imaging can be recorded by computer. It facilitates the operation of selecting a specific sample in the field of view and the accuracy of the experiment. In addition, this stage has good interchangeability and can be easily installed on various types of microscopes such as Zeiss, Nikon, Olympus, and Leica.

Description of drawings

Fig. 1 is the structural representation of the utility model.

Explanation of reference signs

1-stage shell; 2-stepping motor; 3-lead screw; 4-temperature control module; 5-sample position; 6-rotating motor.

Detailed ways

In order to better understand the shape, structure and characteristics of the utility model, preferred embodiments will be listed below and described in detail with reference to the accompanying drawings.

The utility model is an improvement made on the basis of the stage of the patent of the authorized announcement number CN2876813Y. It includes a rectangular stage shell 1 on which the stage 7 is installed and can be rotated on the stage shell 1 . A stepping motor 2 is respectively arranged on the adjacent two sides of the stage shell 1, and two lead screws 3 are respectively matched with the stepping motors 2 on both sides to control the movement of the stage 7 in the stage shell. Move back and forth, left and right in the plane of 1.

A plurality of sample positions 5 are arranged on the stage 7, and the center of each sample position 5 is a light-transmitting hole. In this embodiment, the stage 7 is circular, and a plurality of sample positions 5 are uniformly arranged on the circumference of the stage 7 . The sample position 5 is a small circular stage with a diameter of 3.5 cm, which can place a circular petri dish. In this embodiment, there are 8 sample positions 5, numbered 1-8.

A rotation motor 6 is provided in the middle of the stage 7 to rotate each sample position 5 to a position suitable for observation. A temperature control module 4 is provided below the stage 7, which can realize a constant temperature at any temperature within the range of -20°C to +90°C. The top of the stage 7 can be covered with a rotating disk cover that matches the stage 7 . The stepping motor 2, the rotating motor 6 and the temperature control module 4 are all connected to a computer or a control box, and can receive instructions to perform the above-mentioned relative movement and rotation.

In the actual sample observation, the stage 7 is first positioned at the No. 1 sample position 5, and then the stage 7 moves horizontally and vertically to focus and image according to the instructions issued by the computer or the control box, and records the observed The position of the indicator. After the sample observation of the No. 1 small circular stage is completed, the stage 7 is controlled by the computer or the control box, and automatically rotates to the No. 2 small circular stage, and then moves, focuses and images in the horizontal and vertical directions again. And record the position of the observed index, and so on, until all the samples in the sample position 5 are all observed.

The temperature control module 4 set below the stage 7 is in order to adapt to the application under some specific environments (such as in the cell culture process, continuously observe the cell state changes, screen a specific cell, and the specific temperature has a certain effect on the culture. Influences, etc.), so that the stage 7 can be set to a certain constant temperature (-20 ℃ ~ +90 ℃ arbitrary adjustment), and all parts of the utility model is moisture-proof design, can be used in a long-term humid environment.

The use of the stage of the utility model greatly improves the observation and detection efficiency of medical or biological samples. By using the automatic movement and focusing of the stage, objects in different fields of view and layers in each detection sample can be observed, and by using The computer records the position and imaging, which greatly facilitates the operation of selecting a specific sample in the field of view and the accuracy of the experiment. In addition, this stage has good interchangeability, and can be easily installed on various types of microscopes such as Zeiss, Nikon, Olympus, Leica, etc., and the rotation, horizontal and vertical of the stage can be automatically controlled by the computer software system Moving and focusing imaging, realize the cultivation and automatic continuous amplification observation of multiple cultures on this stage, and record the position of the sample in the culture, realize the function of multi-field automatic movement and automatic focusing, and can be controlled by software Realize functions such as image stitching and target sample positioning.

The above description of the utility model is illustrative rather than restrictive. Those skilled in the art understand that many modifications, changes or equivalents can be made to it within the spirit and scope of the claims, but they will all fall into the Into the scope of protection of the present utility model.

Claims (7)

1. an automatic rotation is located and the objective table of cultivating, it is characterized in that, comprise the objective table shell, objective table is installed on the described objective table shell, be provided with rotation motor in the middle of the described objective table, controlling described objective table rotates on the objective table shell, respectively be provided with a step motor on the adjacent two sides of described objective table shell, two leading screws match with the described step motor of two sides respectively, to control described objective table move left and right after the objective table shell is gone forward, described objective table is provided with a plurality of samples position, and the center of each described sample position is light hole.

2. the objective table of automatic rotation location as claimed in claim 1 and cultivation is characterized in that, described objective table is circular, and a plurality of described samples position evenly is located on the circumference of described objective table.

3. the objective table of automatic rotation location as claimed in claim 1 and cultivation is characterized in that described sample position is the small circular objective table, diameter 3.5cm.

4. the objective table of automatic rotation location as claimed in claim 1 and cultivation is characterized in that the below of described objective table is provided with temperature control module.

5. the objective table of automatic rotation location as claimed in claim 4 and cultivation is characterized in that, described temperature control module is to keep a default constant temperature in-20 ℃～+ 90 ℃ scopes.

6. the objective table of automatic rotation location as claimed in claim 1 and cultivation is characterized in that, the rotation disk cover that is complementary with described objective table is added a cover in described objective table top.

7. the objective table of automatic rotation location as claimed in claim 4 and cultivation is characterized in that described step motor, rotation motor and temperature control module all link to each other with computing machine or control enclosure.