CN201971839U - Potential difference circulatory bioreactor - Google Patents
Potential difference circulatory bioreactor Download PDFInfo
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- CN201971839U CN201971839U CN 201120003914 CN201120003914U CN201971839U CN 201971839 U CN201971839 U CN 201971839U CN 201120003914 CN201120003914 CN 201120003914 CN 201120003914 U CN201120003914 U CN 201120003914U CN 201971839 U CN201971839 U CN 201971839U
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/34—Internal compartments or partitions
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/36—Means for collection or storage of gas; Gas holders
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Abstract
The utility model relates to a potential difference circulatory bioreactor which comprises a container with a gas containing chamber at the upper part of an inner chamber and a liquid containing chamber at the lower part of the inner chamber, the side wall of the container is provided with a flank chamber structure extending to a flank at the connection part of the gas containing chamber and the liquid containing chamber, the inner port of the flank chamber structure is communicated with the inner chamber of the container, the outer port of the flank chamber structure is communicated with a circulating pipe, the lower port of the circulating pipe is communicated with the bottom of the inner chamber of the container, and a one-way venting valve is arranged inside the circulating pipe or at the lower port of the circulating pipe. When any power drives the potential difference circulatory bioreactor to swing left and right, or drives the flank chamber structure to swing up and down, under the action of the one-way venting valve, the liquid level in the upper section of the circulating pipe and the liquid level in the liquid containing chamber form a height difference, and the potential difference can push a culture solution to flow to the bottom of the container trough the circulating pipe at the lower part, and resulting in the circulation of the culture solution between the liquid containing chamber and the circulating pipe, thus realizing sufficient gas-liquid exchange and high-efficient culture solution mixing.
Description
Technical field
The utility model relates to a kind of gravitional force that utilizes and promotes the circulating bio-reactor of nutrient solution round-robin potential difference, this bio-reactor is mainly used in animal cell culture and utilizes zooblast to carry out the production of biological technology products, also can be used for the cultivation of insect cell and vegetable cell and microorganism and the production of biological technology products thereof.
Background technology
Utilize cultured animals cell (comprising the human cell) to carry out life science; cellular transplantation therapy clinical disease and production biological technology products are (as protein; the polypeptide class; the nucleic acid class; antibody class; virus vector and virus vaccines class) production, often need to carry out efficiently the animal cell culture with mass-producing, therefore need research and development and produce the zooblast reactor that can satisfy high-level efficiency and large-scale production.So-called animal bioreactor is exactly to carry out large-scale animal cell culture and utilize the zooblast of these large scale culturing to carry out the device that biological technology products is produced under the artificial conditions of setting such as temperature, PH and dissolved oxygen.Because zooblast poor growth and acellular wall, to environmental change adaptive faculty difference such as temperature and PH and to ventilation with stir the shearing force sensitivity that causes, therefore, when carrying out animal cell culture, will be under suitable temperature and Ph environment, the oxygen (O2) and the nutritive substance of cell fullest are provided, and avoid stirring the shearing damage that causes with gas-filled bubbles to greatest extent.This also is zooblast reactor and other reactors (as microorganism fermentation tank) difference.Scientists is being devoted to the research and development and the production of ideal animal bioreactor always in recent decades, come into the market from being amplified to the existing dissimilar bio-reactors of suitability for industrialized production step by step at present, multi-ply wood reactor for example, the rolling bottle incubator, the plastics bag multiplier (-icator), the spiral membrane reactor, the tubular type helical reactors, the cellular reactor of ceramic rectangular channel, fluidized-bed reactor, hollow fiber reactor, film reactor, stirred reactor, airlift reactor and perfusion type reactor etc., its principle all is to improve the exchange of gas-to-liquid contact and gas-liquid as far as possible to increase the dissolved oxygen of nutrient solution, realizes that with as far as possible little shearing force thorough mixing is to satisfy the demand of cell to oxygen and nutritive substance.Assign to from the growth forms of culturing cell, above-mentioned bio-reactor can be suspension culture formula and adherent culture formula two big classes.Suspension culture formula bio-reactor is mainly used in the suspension culture of non-adherent cell and the suspension culture of attached cell-microcarrier, so-called non-adherent type cell, promptly be to need not to be attached to support surface can grow, comprise blood, Lymphoid tissue cell, many tumour cells and some transformant.This class cell need carry out suspension culture.The serum-free suspension culture is a kind of cell cultures mode that replaces animal serum with the known protein of the people source of purifying or animal-origin or hormone, and it can reduce later stage purifying work, improves the quality of products, and becomes the trend that animal cell large-scale is cultivated just gradually.So-called adherent type cell then is to be attached to the cell that could grow with the support surface of an amount of electric charge, most of zooblasts, and non-Lymphoid tissue cell and many heteroploid cells all belong to this class.
Although various bio-reactors are in structure, the culturing cell type, transmission of power, scale, efficient, shearing force, Operating Complexity, have nothing in common with each other in aspect such as crossed contamination and price, but can form mainly including of main product at home and abroad: the rolling bottle bio-reactor, magnetic agitation bio-reactor, NBS are the cage type bioreactor of representative, WAVE wave bio-reactor and AP torrent formula bio-reactor etc.Wherein the rolling bottle reactor can carry out the attached cell cultivation, has the little advantage of shearing force; The magnetic agitation reactor all can carry out suspension culture to non-adherent cell and attached cell-microcarrier, but the magnetic agitation shearing force is bigger; No matter the rolling bottle reactor still is the magnetic agitation reactor; all can only carry out the small-scale cell cultures or cultivate providing seed to amplify for mass-producing; labour intensity is big; efficient is low; can not mass-producing or perhaps mass production; just be difficult to quality control, there is cross-contamination issue in non-once use in addition.The representative main flow bio-reactor that can carry out large-scale industrial production on the market mainly contains three kinds, and the one, the static perfusion type bio-reactor of NBS, the 2nd, WAVE shakes the pocket type bio-reactor, and the 3rd, pacify general torrent formula reactor.The static perfusion type bio-reactor of NBS is to utilize the oxygenation of nutrient solution bubbling, the stirring arm agitation cycle, cell on the static diaphragm carrier that is grown in the cage is carried out perfusion type to be cultivated, it is little and be suitable for extensive attached cell and cultivate to have shearing force, shortcoming is to be unsuitable for suspension culture, cross conference because of the shearing force that stirs suspension cultured cells is formed damage, and this device structure complexity, also exist the operative technique difficulty big, cost an arm and a leg, the non-once use exists wasting time and energy of cleaning and sterilizing and cross-contamination issue.It is that cell cultures is cultivated in the soft bag of disposable plastic that WAVE shakes the pocket type bio-reactor, produces wave by shaking bag, makes cell suspension, nutrient solution mixes, gaseous interchange, its shearing force is less relatively, is suitable for the suspension culture of non-adherent cell and microcarrier-attached cell.Its main drawback is: this product can not highdensityly be cultivated attached cell, it is reported, at least 10 times to 100 times are lower than the static perfusion type of picture and cultivate.To suspension cultured cells, when small volume is cultivated, can not produce enough waves and make cell suspension, cell deposition at the bottom of bag, phase mutual friction in the process of shaking and damaging, when therefore amplifying on a small scale, often need be by rolling bottle or magnetic agitation culturing bottle.Secondly, the WAVE culture bag of different model will be with the rocker that matches, and pouch portion can use big rocker, and sack can not use little rocker, need buy the equipment of a series of costlinesses through being amplified to large-scale industrial production step by step from the small-scale cultivation.In addition, it is the soft bags of plastics that WAVE shakes bag, adds continuous a series of pipelines, operates also more complicated.Pacify general torrent formula reactor and have the dissolved oxygen height; little and the disposable torrent formula of shearing force reaction bag does not have the advantage of crossed contamination; but only can be used for the static cultivation of mass-producing attached cell; the nutrient solution circulation that utilizes peristaltic pump to produce can not be used for the suspension culture of non-adherent cell, because can be pushed suspension cultured cells repeatedly.This equipment is also expensive, and operation is also complicated.
The utility model content
It is a kind of simple in structure, easy to use that the purpose of this utility model is to provide, and shearing force is little, it is good to mix ventilation effect, not only has been suitable for preparation on a small scale but also can have realized the circulating bio-reactor of potential difference of suitability for industrialized production.
For achieving the above object, the utility model adopts following technical scheme: the circulating bio-reactor of a kind of potential difference, this reactor comprises that the top of inner chamber is for holding air chamber, the bottom is the container body of liquid storage chamber, has the flank cavity structure of extending in the adjoiner that holds air chamber and liquid storage chamber on the sidewall of this container body to the side, the inner port of flank cavity structure is communicated with the inner chamber of container body, be communicated with on the external port of flank cavity structure and be provided with circulation tube, the lower port of circulation tube is communicated with the bottom of the inner chamber of container body, and in the pipeline of circulation tube or lower port place is provided with unidirectional venting valve.
The below that the epimere of described circulation tube extends to the external port place of flank cavity structure by the outer side wall place of container body and is close to flank cavity structure is provided with.
Described unidirectional venting valve structure is plural bag shape flexible valve, and the sack of bag shape flexible valve is towards the inner chamber of container body, and the outer side correspondence of bag shape flexible valve is arranged on the inwall of circulation tube.
Described unidirectional venting valve structure is positioned at the lower port place of circulation tube, unidirectional venting valve structure is the limit diaphragm of the paddle type of coincideing with the lower port shape of circulation tube, the limit diaphragm folding is sticked on the lower port of circulation tube, and the lower port place of circulation tube has the diameter that the is embedded limit diaphragm counterbore greater than the interior caliber of circulation tube.
String is provided with the perfusion post that inside is mounted with the diaphragm carrier that is used to attach cell on the described circulation tube.
The top of the inner chamber of described container body is that oval column, bottom are the semielliptical shape, and described flank cavity structure is two, lays respectively at the two ends of the major axis of container body.
The upper end open of described appearance air chamber is provided with container cover, and the sidewall that holds air chamber is provided with ventilative window, and ventilative window is provided with the bacteriological filtration film.
Sealing is provided with container cover on the upper end open of described appearance air chamber, hold be communicated with on the sidewall of air chamber be provided with the air inlet mouth of pipe, the mouth of pipe of giving vent to anger, the liquid in-out mouth of pipe and sampling nozzle, PH susceptor interface and DO susceptor interface.
Described flank cavity structure and circulation tube epimere can swing up and down with respect to the container body.
The sidewall of described appearance air chamber has the elastic laminates section that can stretch up and down, and the sidewall that holds air chamber is provided with the swing arm that the other end is connected with the external end head of flank cavity structure in the top of telescopic section.
Has the flank cavity structure of extending at appearance air chamber and liquid storage chamber's adjoiner on the sidewall of container body of the present utility model to the side, the inner port of flank cavity structure is communicated with the inner chamber of container body, the external port of flank cavity structure is connected with circulation tube, the lower port of circulating line is communicated with the bottom of the inner chamber of container body, and in the pipeline of circulation tube or the lower port place of circulation tube is provided with unidirectional venting valve.When any power wheel drive the utility model is vacillated now to the left, now to the right, when perhaps driving flank cavity structure and swinging up and down, under the effect of unidirectional venting valve, can cause the liquid level in circulation tube epimere and the liquid storage chamber to form difference of altitude, just the formation gravitional force of nutrient solution is poor in nutrient solution in the circulation tube epimere and the liquid storage chamber, the gravitional force that this gravitional force difference is saved can promote nutrient solution and flow to container bottom through the bottom circulating line, and the nutrient solution that is in the liquid storage chamber bottom originally can upwards turn over from the bottom, cause nutrient solution circulation repeatedly between liquid storage chamber and circulating line, thereby realize sufficient gas-liquid exchange and nutrient solution mixing efficiently, this circulation is simply effective, it is good to mix ventilation effect, circulation shear power is minimum, and it is good to mix ventilation effect, can suspension culture can staticly cultivate again, not only can be suitable for preparation on a small scale but also can realize suitability for industrialized production.Hold air chamber and facing of liquid storage chamber meet the place because flank cavity structure of the present utility model is positioned at, when practicality, the liquid level of nutrient solution can be in the middle part of flank cavity structure inner chamber, like this top aerification of flank cavity, and the bottom is full of nutrient solution, can increase the exchange gas area.Further strengthen ventilation effect.
Unidirectional venting valve structure of the present utility model is plural bag shape flexible valve, the sack of bag shape flexible valve is towards the port of lower opening, bag shape flexible valve is arranged on the inwall of circulating line by a side, drive reactor-side of the present utility model to waving in external force, perhaps drive in the process that flank cavity structure swings up and down, when flank cavity locations of structures reduces, the inner chamber of the calm body of nutrient solution meeting enters the inner chamber of flank cavity structure, enter in the circulating line then, and the nutrient solution that is positioned at the lower port inboard of the circulating line bottom the liquid storage chamber this moment is full of the flexible valve bag, bag shape flexible valve expands lower port is shut, when flank cavity locations of structures raises, nutrient solution squeeze bag shape flexible valve in the circulating line, the liquid in the bag shape flexible valve is extruded, bag shape flexible valve smaller volume, nutrient solution will be discharged by lower opening, and this valve is a biomimetic features, unidirectional through-flow reliability height, bag shape flexible flaps film body is flexible, and shearing force is minimum.
The upper end open of container body of the present utility model is provided with container cover, be communicated with at interval on the sidewall of appearance air chamber and be provided with inlet mouth and air outlet, when the volume of culture vessel is big, nutrient solution is when only dissolved oxygen can't be satisfied the demand by gaseous interchange in culture vessel upper end ventilation mouth and the incubator, can be by inlet mouth oxygenation in the culture vessel, discharge the waste gas that organism produces by the air outlet simultaneously, can well guarantee the vegetative activity of organism.
String is provided with the perfusion post on the circulation tube of the present utility model, attaches attached cell in the perfusion post, just can carry out static perfusion and cultivate, when carrying out suspension culture, the perfusion post removed and get final product, therefore the utility model both can carry out suspension culture, can carry out static perfusion again and cultivate, and subject range is extensive.
Description of drawings
Fig. 1 is the structural representation of the front view of the utility model embodiment 1;
Fig. 2 is the left view of Fig. 1;
Fig. 3 is the vertical view of Fig. 1;
Fig. 4 is the structural representation behind the base of adding of the utility model embodiment 1;
Fig. 5 is the stereographic map at a visual angle of bag shape flexible valve among Fig. 1;
Fig. 6 is the structural representation of bag shape flexible valve among Fig. 1;
Fig. 7 is the fundamental diagram of bag shape flexible valve among Fig. 1;
Fig. 8 is the another kind of form of the unidirectional venting valve structure of the utility model embodiment 1;
Fig. 9 is the fundamental diagram of another kind of form of the unidirectional venting valve structure of the utility model embodiment 1;
Figure 10 is the structural representation of the front view of the utility model embodiment 2;
Figure 11 is the structural representation of the front view of the utility model embodiment 3;
Figure 12 is the structural representation of the front view of the utility model embodiment 4.
Embodiment
The embodiment 1 of the circulating bio-reactor of a kind of potential difference, in Fig. 1 ~ 7, this reactor comprises the container body, and the inner chamber of container body is divided into two portions up and down, and top is liquid storage chamber 2 for appearance air chamber 1, bottom.Have two flank cavity structures 4 of extending on the sidewall of container body to both sides, flank cavity structure 4 is positioned at the adjoiner that holds air chamber and liquid storage chamber, flank cavity structure 4 has inside and outside two ports, the inner port of flank cavity structure 4 is communicated with the inner chamber of container body, the external port of flank cavity structure 4 is communicated with the upper port of circulation tube 5, the below that the epimere of circulation tube 5 is close to flank cavity structure 4 is provided with, the upper end of circulation tube 5 extends to the external port place of flank cavity structure 4 by the side-walls of container body, and the external port of flank cavity structure 4 is divided a word with a hyphen at the end of a line with the upper port of circulation tube 5 and is communicated with.The interlude of circulation tube 5 and hypomere move down along the outer side wall of container body, and the lower port of circulation tube 5 is communicated with the bottom of the inner chamber of container body.The inboard that the lower port place of circulation tube 5 is provided with is provided with marsupial shape flexible valve 7, bag shape flexible flaps is three, the sack of bag shape flexible valve 7 is towards the inner chamber of container body, the bag top is towards the inside of circulation tube 5, three bag shape flexible valve 7 are distributed on the lower port place of circulation tube 5 and with the lower port of circulation tube 5 obturages, the outer side that bag shape flexible valve 7 is adjacent with the tube wall of circulation tube 5 is fixedly installed on the tube wall of circulation tube 5, bag shape flexible valve is a biomimetic features, copy the endovascular valve designs of organism, bag shape flexible valve can be semi-moon shaped pocket-like, also can be the pocket-like of semielliptical shape.Fig. 7 is the one-way flow schematic diagram of bag shape flexible valve, when liquid stream flows to sack by the bag top, liquid in the bag shape flexible valve will be extruded, each bag shape flexible valve is pressed against the tube wall place of circulation tube 5, and liquid stream is flow through by the center of circulation tube, when liquid stream is backflowed when bag pushes up by sack, in the bag shape flexible valve with full of liquid, liquid is big with the support of bag shape flexible valve, and each bag shape flexible valve 7 is sealed up the lower port of circulation tube 5, thereby realizes the purpose of one-way flow.
The below of container body is provided with base 17, base 17 is flat, the length direction of base 17 is consistent with the direction of extension of flank cavity structure 4, both sides at the width of base 17 are provided with support 16, the lower end of support 16 is fixed on the base 17, the upper end of support 16 is hinged on by rotational pin 15 on the sidewall of container body, and like this, the container body can rotate with respect to base.When needs container body is swung with base 17, rotational pin 15 can be fixed on the support 16, also rotational pin and container body are fixed simultaneously, stop the container body to rotate with respect to base.
Unidirectional venting valve structure in the present embodiment is a bag shape flexible valve, unidirectional venting valve also can adopt structure as shown in Figure 8, in Fig. 8, the be sticked limit diaphragm 13 of paddle type of the outside of the lower port of circulation tube 5, the outer rim of limit diaphragm 13 is greater than the internal diameter of the lower port of circulation tube 5, the lower port place of circulation tube 5 has the counterbore of diameter greater than the interior caliber of circulation tube 5, limit diaphragm 13 is embedded in this counterbore, this counterbore can be arranged on the end face of lower port of circulation tube 5, also can be arranged on the chamber wall of inner chamber of container body.Fig. 9 is limit diaphragm 13 one-way flow schematic diagrams, when in liquid flows by the lateral of circulation tube, flowing, the pressure of liquid stream can be pressed on limit diaphragm 13 on the bottom surface of counterbore at lower port place of circulation tube 5, closing of the lower port of realization circulation tube, when liquid stream is outwards flowed by the inboard of circulation tube, liquid fails to be convened for lack of a quorum and presses off the limit diaphragm 13 that is sticked on the lower port of circulation tube 5, the opening of the lower port of circulation tube, and liquid stream passes through smoothly.
Hold as the improvement of present embodiment that sealing is provided with container cover 6 on the upper end open of air chamber 1, hold be communicated with on the sidewall of air chamber 1 be provided with the air inlet mouth of pipe 9, the mouth of pipe 10 of giving vent to anger, the feed liquor mouth of pipe 8 and the fluid mouth of pipe 3, PH susceptor interface 11 and DO susceptor interface 12 and sampling nozzle 14.The air inlet mouth of pipe 9, the mouth of pipe 10 of giving vent to anger, the feed liquor mouth of pipe 8 and the fluid mouth of pipe 3, PH susceptor interface 11 and DO susceptor interface 12 and and sampling nozzle 14 all with hold air chamber 1 and be communicated with.
The embodiment 2 of the circulating bio-reactor of a kind of potential difference, in Figure 10, the difference of present embodiment and embodiment 1 is: the pars intermedia bit string of the circulation tube 5 in the present embodiment is provided with perfusion post 20, perfusion post 20 is a stylostome, the inside of perfusion post 20 is mounted with the diaphragm carrier, and the diaphragm carrier is used for attaching for cell.The perfusion post is existing a kind of structure of cultivating attached cell.Other structure of the circulating bio-reactor of potential difference in the present embodiment is the same with embodiment 1 corresponding construction.
The embodiment 3 of the circulating bio-reactor of a kind of potential difference, in Figure 11, the difference of present embodiment and embodiment 1 is: be installed with perfusion post 19 in the present embodiment in the inner chamber of container body, the cavity that perfusion post 19 coincide for the inner chamber for profile and container body, reprint the inside of perfusion post 19 has diaphragm carrier, diaphragm carrier to be used for attaching for cell.The perfusion post is existing a kind of structure of cultivating attached cell.Other structures of the circulating bio-reactor of potential difference in the present embodiment are the same with embodiment 1 corresponding construction.
The embodiment 4 of the circulating bio-reactor of a kind of potential difference, in Figure 12, the difference of present embodiment and embodiment 1 is: have the elastic laminates section 23 that an end can stretch up and down on the sidewall of the appearance air chamber 1 of present embodiment, one end of swing arm 22 is hinged to be arranged on the sidewall that holds air chamber 1, swing arm 22 is positioned at the top of elastic laminates section 23 at appearance air chamber 1 sidewall upper link point, the other end of swing arm 22 is hinged to be arranged on the external end head of flank cavity structure 4, flank cavity structure 4 is a flexible materials with the junction of container body, and flank cavity structure 4 can be swung with respect to the container body.Other structures of the circulating bio-reactor of potential difference in the present embodiment are the same with embodiment 1 corresponding construction.Be hinged setting between the swing arm of present embodiment and container body and the flank cavity configuration, when container body and flank cavity configuration are softer silica gel, when latex glue material or plastics were made, swing arm and container chamber and flank cavity structure also can directly be fixed.Swing arm in the present embodiment also can replace with lifting silk.
Flank cavity configuration among the above embodiment and circulation tube turn back at the external port place of flank cavity configuration for being wholely set, and the section of turning back is the epimere of circulation tube.
The top of the inner chamber of container body is that oval column, bottom are the semielliptical shape among the above embodiment, and two flank cavity structures lay respectively at the two ends of the major axis of container body.
Be provided with the air inlet mouth of pipe on the appearance gas chamber sidewall among the above embodiment, the mouth of pipe of giving vent to anger, the feed liquor mouth of pipe, the fluid mouth of pipe and sampling nozzle, PH susceptor interface and DO(oxygen dissolved) the susceptor interface, when the volume of container body hour, these mouthfuls need be set on the sidewall that holds air chamber.At this moment only on the sidewall that holds air chamber, ventilative window is set, now takes a breath,, can on ventilative window, the bacteriological filtration film be set in order to prevent the nutrient solution in the extraneous bacterial contamination container intracoelomic cavity by the transom cause for gossip.
Packed flexible valve among the above embodiment is arranged on the lower port place of circulation tube, also packed flexible valve can be arranged in the tube chamber of circulation tube.
Packed flexible valve among the above embodiment can be wholely set on circulation tube.
Flank cavity structure among the above embodiment can be arranged to oblate shape, and the epimere of corresponding circulation tube is positioned at the oblate shape that flank cavity structural shape adapts to.
The bag shape flexible valve of above embodiment is two, also can be for more.
The below that the epimere of the circulation tube among the above embodiment is close to flank cavity structure is provided with, and the below that also can not be close to flank cavity structure is provided with, and circulation tube has the external port place of flank cavity structure directly to hang down.
The liquid storage chamber of the container body of above embodiment and the chamber face of circulating line are smooth camber line shape and smooth surface, when flowing, nutrient solution produces as far as possible little shearing force, the flexible unidirectional venting valve of bag shape of bionical thing cardiovascular valve has been proved to be the shearing force that the blood circulation cell is had minimum; The shearing force that circulation produced that promotes by gravitional force stirs more than mechanicalness, and the shearing force that wave mixes and the peristaltic pump circulation is produced is little.
Be that a kind of gravitional force that utilizes promotes the circulating bio-reactor of nutrient solution round-robin potential difference among the above embodiment, this reactor is from bionic angle design.
When the circulating bio-reactor of the potential difference in above embodiment is worked, can swing with respect to the container body together with the epimere of circulation tube by various power wheel drive reactors swings or the flank cavity structure that drives reactor.When the epimere swing that drives flank cavity structure and circulation tube, can adopt the outer end of the piston roof pressure flank cavity structure of hydro-cylinder, make it upwards put and make its bottom under the effect of gravity, with flank cavity structure outer section of living directly is fixed on the piston rod of hydro-cylinder, allow piston rod in the process that moves up and down, drive flank cavity structure and swing up and down, form and drive liquid stream round-robin potential difference together with the epimere of circulation tube.Also can be provided with on the container body fixing on driving gear, driving gear and transmitting gear engagement, transmitting gear are by electric motor driving, motor drives the container body by rotating and swings.Can also be on the container body fixing horizontal lever, drive the horizontal lever swing, realize the swing of container body.
Life science is told us, from the lower animal to the mankind, from animalcule to huge elephant, the terricole dinosaur that body weight is 130 tons, the whale that surpasses 200 tons of weights, none does not provide oxygen and nutritive substance by circulation for body, and therefore, circulation provides the exchange efficiency of oxygen and nutritive substance to want high more than mixing.Animal is realized circulation, and by the power-driven pump of this shearing force minimum of heart, and the most critical structure of formation way flow is the minimum cardiovascular valve of shearing force.The activity of animal hearts and cardiovascular valve to all the time all not have to damage in circulation and to the highstrung red corpuscle of shearing force, show that its shearing force is minimum, and the artificial mechanical valve prosthesis's of heart (wear-resisting reach 100 years) successful clinical application, it is feasible that enlightenment utilizes the artificial mechanical valve prosthesis to research and develop circulating bio-reactor as the way flow valve of bio-reactor, utilize run by gravity for impellent also more than the stirring arm of using with present bio-reactor, the shearing force that wave and peristaltic pump produced is much smaller.
Claims (10)
1. circulating bio-reactor of potential difference, it is characterized in that: this reactor comprises that the top of inner chamber is the container body of liquid storage chamber for appearance air chamber, bottom, has the flank cavity structure of extending in the adjoiner that holds air chamber and liquid storage chamber on the sidewall of this container body to the side, the inner port of flank cavity structure is communicated with the inner chamber of container body, be communicated with on the external port of flank cavity structure and be provided with circulation tube, the lower port of circulation tube is communicated with the bottom of the inner chamber of container body, and in the pipeline of circulation tube or lower port place is provided with unidirectional venting valve.
2. the circulating bio-reactor of potential difference according to claim 1 is characterized in that: the below that the epimere of described circulation tube extends to the external port place of flank cavity structure by the outer side wall place of container body and is close to flank cavity structure is provided with.
3. the circulating bio-reactor of potential difference according to claim 1, it is characterized in that: described unidirectional venting valve structure is plural bag shape flexible valve, the sack of bag shape flexible valve is towards the inner chamber of container body, and the outer side correspondence of bag shape flexible valve is arranged on the inwall of circulation tube.
4. the circulating bio-reactor of potential difference according to claim 1, it is characterized in that: described unidirectional venting valve structure is positioned at the lower port place of circulation tube, unidirectional venting valve structure is the limit diaphragm of the paddle type of coincideing with the lower port shape of circulation tube, the limit diaphragm folding is sticked on the lower port of circulation tube, and the lower port place of circulation tube has the diameter that the is embedded limit diaphragm counterbore greater than the interior caliber of circulation tube.
5. according to any circulating bio-reactor of the described potential difference of claim in the claim 1 ~ 4, it is characterized in that: string is provided with the perfusion post that inside is mounted with the diaphragm carrier that is used to attach cell on the described circulation tube.
6. the circulating bio-reactor of potential difference according to claim 1 is characterized in that: the top of the inner chamber of described container body is that oval column, bottom are the semielliptical shape, and described flank cavity structure is two, lays respectively at the two ends of the major axis of container body.
7. the circulating bio-reactor of potential difference according to claim 1, it is characterized in that: the upper end open of described appearance air chamber is provided with container cover, and the sidewall that holds air chamber is provided with ventilative window, and ventilative window is provided with the bacteriological filtration film.
8. the circulating bio-reactor of potential difference according to claim 1, it is characterized in that: sealing is provided with container cover on the upper end open of described appearance air chamber, hold be communicated with on the sidewall of air chamber be provided with the air inlet mouth of pipe, the mouth of pipe of giving vent to anger, the liquid in-out mouth of pipe and sampling nozzle, PH susceptor interface and DO susceptor interface.
9. the circulating bio-reactor of potential difference according to claim 1 is characterized in that: described flank cavity structure and circulation tube epimere can swing up and down with respect to the container body.
10. the circulating bio-reactor of potential difference according to claim 9, it is characterized in that: the sidewall of described appearance air chamber has the elastic laminates section that can stretch up and down, and the sidewall that holds air chamber is provided with the swing arm that the other end is connected with the external end head of flank cavity structure in the top of telescopic section.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201120003914 CN201971839U (en) | 2011-01-07 | 2011-01-07 | Potential difference circulatory bioreactor |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201120003914 CN201971839U (en) | 2011-01-07 | 2011-01-07 | Potential difference circulatory bioreactor |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102154100A (en) * | 2011-01-07 | 2011-08-17 | 郑州威瑞生物技术有限公司 | Potential difference circulating bioreactor |
| CN114874878A (en) * | 2022-07-11 | 2022-08-09 | 浙江金仪盛世生物工程有限公司 | Support frame with two clamping states and biological reaction device |
| CN114907978A (en) * | 2022-05-23 | 2022-08-16 | 江南大学 | Low-shear perfusion culture system and method suitable for high-density mammalian cell culture |
-
2011
- 2011-01-07 CN CN 201120003914 patent/CN201971839U/en not_active Expired - Lifetime
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102154100A (en) * | 2011-01-07 | 2011-08-17 | 郑州威瑞生物技术有限公司 | Potential difference circulating bioreactor |
| CN114907978A (en) * | 2022-05-23 | 2022-08-16 | 江南大学 | Low-shear perfusion culture system and method suitable for high-density mammalian cell culture |
| CN114874878A (en) * | 2022-07-11 | 2022-08-09 | 浙江金仪盛世生物工程有限公司 | Support frame with two clamping states and biological reaction device |
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