CN201408192Y - LSPR sensor chip for detecting blood coagulation factor and D dipolymer in blood - Google Patents
LSPR sensor chip for detecting blood coagulation factor and D dipolymer in blood Download PDFInfo
- Publication number
- CN201408192Y CN201408192Y CN2009200506963U CN200920050696U CN201408192Y CN 201408192 Y CN201408192 Y CN 201408192Y CN 2009200506963 U CN2009200506963 U CN 2009200506963U CN 200920050696 U CN200920050696 U CN 200920050696U CN 201408192 Y CN201408192 Y CN 201408192Y
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- clotting factor
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- 239000003114 blood coagulation factor Substances 0.000 title claims abstract description 42
- 102000015081 Blood Coagulation Factors Human genes 0.000 title claims abstract description 38
- 108010039209 Blood Coagulation Factors Proteins 0.000 title claims abstract description 38
- 239000008280 blood Substances 0.000 title abstract description 8
- 210000004369 blood Anatomy 0.000 title abstract description 8
- 229940019700 blood coagulation factors Drugs 0.000 title abstract 5
- 229910052751 metal Inorganic materials 0.000 claims abstract description 35
- 239000002184 metal Substances 0.000 claims abstract description 35
- 239000013307 optical fiber Substances 0.000 claims abstract description 5
- 239000003154 D dimer Substances 0.000 claims description 20
- 239000000758 substrate Substances 0.000 claims description 18
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 claims description 9
- 229910052710 silicon Inorganic materials 0.000 claims description 9
- 239000010703 silicon Substances 0.000 claims description 9
- 238000001514 detection method Methods 0.000 claims description 7
- 108010000487 High-Molecular-Weight Kininogen Proteins 0.000 claims description 5
- 108090000113 Plasma Kallikrein Proteins 0.000 claims description 5
- BTCSSZJGUNDROE-UHFFFAOYSA-N gamma-aminobutyric acid Chemical group NCCCC(O)=O BTCSSZJGUNDROE-UHFFFAOYSA-N 0.000 claims description 5
- RTAQQCXQSZGOHL-UHFFFAOYSA-N Titanium Chemical group [Ti] RTAQQCXQSZGOHL-UHFFFAOYSA-N 0.000 claims description 3
- 239000010936 titanium Substances 0.000 claims description 3
- 229910052719 titanium Inorganic materials 0.000 claims description 3
- 102000002261 High-Molecular-Weight Kininogen Human genes 0.000 claims 1
- 230000023555 blood coagulation Effects 0.000 abstract description 8
- 239000005543 nano-size silicon particle Substances 0.000 abstract 3
- 238000000034 method Methods 0.000 description 5
- 102100035792 Kininogen-1 Human genes 0.000 description 4
- 239000003527 fibrinolytic agent Substances 0.000 description 4
- 230000003480 fibrinolytic effect Effects 0.000 description 4
- 208000032843 Hemorrhage Diseases 0.000 description 3
- BWGVNKXGVNDBDI-UHFFFAOYSA-N Fibrin monomer Chemical group CNC(=O)CNC(=O)CN BWGVNKXGVNDBDI-UHFFFAOYSA-N 0.000 description 2
- 108010088842 Fibrinolysin Proteins 0.000 description 2
- 208000007536 Thrombosis Diseases 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 229940001501 fibrinolysin Drugs 0.000 description 2
- 239000013589 supplement Substances 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 102100030563 Coagulation factor XI Human genes 0.000 description 1
- 208000005189 Embolism Diseases 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108010074864 Factor XI Proteins 0.000 description 1
- 108010080805 Factor XIa Proteins 0.000 description 1
- 108010073385 Fibrin Proteins 0.000 description 1
- 102000009123 Fibrin Human genes 0.000 description 1
- 208000031220 Hemophilia Diseases 0.000 description 1
- 208000009292 Hemophilia A Diseases 0.000 description 1
- 206010019663 Hepatic failure Diseases 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 208000001435 Thromboembolism Diseases 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000000740 bleeding effect Effects 0.000 description 1
- 239000012503 blood component Substances 0.000 description 1
- 210000004204 blood vessel Anatomy 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 230000015271 coagulation Effects 0.000 description 1
- 238000005345 coagulation Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940088598 enzyme Drugs 0.000 description 1
- 229950003499 fibrin Drugs 0.000 description 1
- 108010073651 fibrinmonomer Proteins 0.000 description 1
- 230000020764 fibrinolysis Effects 0.000 description 1
- 230000003760 hair shine Effects 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 239000003547 immunosorbent Substances 0.000 description 1
- 208000007903 liver failure Diseases 0.000 description 1
- 231100000835 liver failure Toxicity 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 230000035935 pregnancy Effects 0.000 description 1
- 208000037920 primary disease Diseases 0.000 description 1
- 235000004252 protein component Nutrition 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 208000011580 syndromic disease Diseases 0.000 description 1
- 230000002537 thrombolytic effect Effects 0.000 description 1
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- Investigating Or Analysing Materials By Optical Means (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The utility model discloses an LSPR sensor chip for detecting blood coagulation factor and D dipolymer in blood, by combining optical fiber and a spectrometer, the blood coagulation factor and D dipolymer are detected quantitatively; the chip is provided with a basal plate, two metal layers, one nano-silicon layer and a sensitive layer, the metal layers are arranged on the basal plate, the nano-silicon layer is arranged on the metal layers, and the metal layer is re-arranged on the nano-silicon layer, the sensitive layer is arranged on the metal layer, and sensitive factors reacted with the blood coagulation factor or D dipolymer are arranged on the sensitive layer. The LSPR sensor chip can comprehensively detect the blood coagulation factor and D dipolymer in flood, furthermore, the LSPRsensor chip can judge the function of blood coagulation rapidly and sensitively.
Description
Technical field
The utility model belongs to blood coagulation Function detection technical field, relates in particular to a kind of be used to detect clotting factor and the dimeric chip of D.
Background technology
Clotting factor is the range protein component that participates in the blood clotting process.Its physiological action is, when angiorrbagia, be activated and PA together and mend leak on the plug blood vessel, this process is called as blood coagulation.Found successively in the last hundred years clotting factor is a lot.
Because wound and operation massive haemorrhage are lost clotting factor; Primary disease such as liver failure, hemophilia also makes the synthetic minimizing of clotting factor, influences coagulation function, and hemorrhage being difficult for ends; Blood component defeated during treatment is incomplete, and the clotting factor deficiency also causes bleeding.During supplement of coagulation factors, need to measure the content of clotting factor, the method of measuring clotting factor now mostly is ELISA (enzyme connects immunosorbent and measures) method, finding speed is slow, the reagent costliness that consumes, the blood sample that needs is originally many, and the content of a certain clotting factor of only single mensuration lacks directive significance for kind that instructs supplement of coagulation factors and dosage.
The D dimer be the activated factor XI, plasma thromboplastin antecedent II of fibrin monomer crosslinked after, a species specificity catabolite that is produced through the fibrinolysin hydrolysis is a specific fibrinolytic process label again.The D dimer derives from the crosslinked fibrin grumeleuse of fibrinolysin dissolving.It is a test understanding Secondary cases fibrinolysis function that the plasma D dimer is measured.Measure the main factor of fibrinolytic system, reach and fibrinolytic system diseases related (as tumour, pregnancy syndrome) for Clinics and Practices fibrinolytic system disease (as DIC, various thrombus), and the thromboembolism treatment monitoring, have great significance.But the thrombolysis effect of the dimeric detection by quantitative quantitative response of D-medicine and can be used for diagnosing, screening the thrombus of new formation.The D dimer is the index of hyperfibrinolysis, and D dimer content height illustrates hyperfibrinolysis.
The utility model content
The technical problems to be solved in the utility model provides the chip that a kind of blood testing is used, and can detect clotting factor and D dimer in the blood rapidly with this chip.
For addressing the above problem, the technical solution of the utility model is: a kind of chip that is used for detecting blood clotting factor and the dimeric LSPR sensor of D, by combining clotting factor and D dimer are carried out detection by quantitative with optical fiber and spectrometer, this chip has a substrate, two metal levels, a nanometer silicon layer and a sensitive layer, metal level is set on the substrate, the nanometer silicon layer is set on the metal level, metal level is set on the nanometer silicon layer again, on this metal level sensitive layer is set, this sensitive layer is made up of the sensitive factor that clotting factor or D dimer are reacted.
Sensitive factor on the sensitive layer is the antibody of clotting factor, people's activated clotting factor XII (FXIIa), D dimer, kallikreinogen, high-molecular-weight kininogen or the vWF factor, and described clotting factor is factor I, II, III, V, VI, VII, VIII, IX, X, XI, XII or XIII.
Sensitive factor on the sensitive layer is the combination of the antibody of human blood coagulation, people's activated clotting factor XII (FXIIa), D dimer, kallikreinogen, high-molecular-weight kininogen or the vWF factor, and described human blood coagulation is the combination of factor I, II, III, V, VI, VII, VIII, IX, X, XI, XII or XIII.
The bottom of described substrate is distributed with the sphere pit.Be distributed with sphere pit or the relative sphere pit of spherical surface hill on the described metal level with B.B.P.
The bottom of described substrate is distributed with spherical surface hill.Be distributed with sphere pit or the relative spherical surface hill of spherical surface hill on the described metal level with B.B.P.
Metal level on the described substrate is golden film.
The upper strata of the metal level under the described sensitive layer is golden film, and lower floor is a titanium film.
The utility model can detect clotting factor and the D dimer in the blood more all sidedly, and quick, sensitive.
Description of drawings
Fig. 1 is the structural representation of first embodiment of the utility model;
Fig. 2 is the front view of Fig. 1;
Fig. 3 is the front view of second embodiment of the utility model;
Fig. 4 is the front view of the 3rd embodiment of the utility model;
Fig. 5 is the front view of the 4th embodiment of the utility model;
Fig. 6 is the front view of the 4th embodiment of the utility model;
Fig. 7 is the upward view of Fig. 3
Fig. 8 is the vertical view of Fig. 6;
Fig. 9 is a utilization synoptic diagram of the present utility model.
Embodiment
The utility model is a kind of chip that is used for detecting blood clotting factor and the dimeric LSPR sensor of D, and it carries out detection by quantitative by combining with optical fiber and spectrometer to clotting factor and D dimer.
As shown in Figure 1, 2, this chip has a substrate 1, nanometer silicon layer 3, sensitive layer 5 and two metal levels 2,4, metal level 2 is set on the substrate 1, nanometer silicon layer 3 is set on the metal level 2, metal level 4 is set on the nanometer silicon layer 3 again, metal level 4 is provided with sensitive layer 5, and sensitive layer 5 is made up of the sensitive factor 51 that clotting factor or D dimer are reacted.
Sensitive factor on the sensitive layer is the antibody of clotting factor, people's activated clotting factor XII (FXIIa), D dimer, kallikreinogen, high-molecular-weight kininogen or the vWF factor, and clotting factor is factor I, II, III, V, VI, VII, VIII, IX, X, XI, XII or XIII.Clotting factor or the D dimer of such chip in can single detection blood.
Sensitive factor on the sensitive layer is the combination of the antibody of human blood coagulation, people's activated clotting factor XII (FXIIa), D dimer, kallikreinogen, high-molecular-weight kininogen or the vWF factor, and human blood coagulation is the combination of factor I, II, III, V, VI, VII, VIII, IX, X, XI, XII or XIII.Such chip can detect each known clotting factor and the D dimer in the blood.
Shown in Fig. 3,7, as different from Example 1, the bottom of substrate 1 is distributed with sphere pit 6.
As shown in Figure 4, as different from Example 2, be distributed with the sphere pit 7 relative on the metal level 4 with the sphere pit 6 of substrate 1 bottom.
As shown in Figure 5, as different from Example 2, be distributed with the spherical surface hill 8 relative on the metal level 4 with the sphere pit 6 of substrate 1 bottom.
As shown in Figure 6, as different from Example 4, what the bottom of substrate 1 distributed is spherical surface hill 13.
Fig. 8 is the vertical view of Fig. 6, and as shown in the figure, the sensitive factor 51 of sensitive layer is distributed on each projection 8 of metal level.Fig. 8 also can be Fig. 4,5 vertical view, and then the sensitive factor 51 of sensitive layer is distributed on each pit 8 of metal level.
In embodiment 2,3,4, sphere pit or spherical surface hill on substrate or metal level can play the effect of converging to light, improve the sensitivity that detects.
Fig. 9 illustrates utilization synoptic diagram of the present utility model.The light that light source 11 sends shines on the utility model by optical fiber 9, and reflected back is received by spectrometer 10 again, carries out Treatment Analysis by computing machine 12, realizes clotting factor and the dimeric detection by quantitative of D.
Claims (8)
1. chip that is used for detecting blood clotting factor and the dimeric LSPR sensor of D, by combining clotting factor and D dimer are carried out detection by quantitative with optical fiber (9) and spectrometer (10), it is characterized in that: this chip has a substrate (1), two metal levels (2,4), a nanometer silicon layer (3) and a sensitive layer (5), metal level (2) is set on the substrate (1), nanometer silicon layer (3) is set on the metal level (2), a metal level (4) is set on the nanometer silicon layer (3) again, sensitive layer (5) is set on the metal level (4), and described sensitive layer (5) is made up of the sensitive factor (51) that clotting factor or D dimer are reacted.
2. chip according to claim 1, it is characterized in that: the sensitive factor (51) on the described sensitive layer (5) is the antibody of clotting factor, people's activated clotting factor XII (FXIIa), D dimer, kallikreinogen, high-molecular-weight kininogen or the vWF factor, and described clotting factor is factor I, II, III, V, VI, VII, VIII, IX, X, XI, XII or XIII.
3. chip according to claim 1 is characterized in that: the bottom of described substrate (1) is distributed with sphere pit (6).
4. chip according to claim 1 is characterized in that: the bottom of described substrate (1) is distributed with spherical surface hill (13).
5. according to claim 4 or 5 described chips, it is characterized in that: be distributed with sphere pit (6) or the relative sphere pit (7) of spherical surface hill (13) on the described metal level (4) with substrate (1) bottom.
6. according to claim 4 or 5 described chips, it is characterized in that: be distributed with sphere pit (6) or the relative spherical surface hill (8) of spherical surface hill (13) on the described metal level (4) with substrate (1) bottom.
7. chip according to claim 1 is characterized in that: described metal level (2) is golden film.
8. chip according to claim 1 is characterized in that: the upper strata of described metal level (4) is golden film, and lower floor is a titanium film.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009200506963U CN201408192Y (en) | 2009-01-22 | 2009-01-22 | LSPR sensor chip for detecting blood coagulation factor and D dipolymer in blood |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009200506963U CN201408192Y (en) | 2009-01-22 | 2009-01-22 | LSPR sensor chip for detecting blood coagulation factor and D dipolymer in blood |
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| Publication Number | Publication Date |
|---|---|
| CN201408192Y true CN201408192Y (en) | 2010-02-17 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2009200506963U Expired - Fee Related CN201408192Y (en) | 2009-01-22 | 2009-01-22 | LSPR sensor chip for detecting blood coagulation factor and D dipolymer in blood |
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| Country | Link |
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| CN (1) | CN201408192Y (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104073425A (en) * | 2014-06-17 | 2014-10-01 | 深圳威芯华创科技有限公司 | LSPR (localized surface plasma resonance) sensing device and preparation method thereof as well as DNA detection method |
| CN104792747A (en) * | 2014-01-22 | 2015-07-22 | 希华晶体科技股份有限公司 | Sensing method |
| US9778183B2 (en) | 2015-08-20 | 2017-10-03 | Industrial Technology Research Institute | Sensing chip |
| CN109358037A (en) * | 2018-10-23 | 2019-02-19 | 大连理工大学 | Heterogeneous two-nanoparticle structures insensitive to excitation light polarization state and their applications |
-
2009
- 2009-01-22 CN CN2009200506963U patent/CN201408192Y/en not_active Expired - Fee Related
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104792747A (en) * | 2014-01-22 | 2015-07-22 | 希华晶体科技股份有限公司 | Sensing method |
| CN104073425A (en) * | 2014-06-17 | 2014-10-01 | 深圳威芯华创科技有限公司 | LSPR (localized surface plasma resonance) sensing device and preparation method thereof as well as DNA detection method |
| US9778183B2 (en) | 2015-08-20 | 2017-10-03 | Industrial Technology Research Institute | Sensing chip |
| CN109358037A (en) * | 2018-10-23 | 2019-02-19 | 大连理工大学 | Heterogeneous two-nanoparticle structures insensitive to excitation light polarization state and their applications |
| CN109358037B (en) * | 2018-10-23 | 2020-12-11 | 大连理工大学 | Heterogeneous two-nanoparticle structures insensitive to excitation light polarization state and their applications |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20100217 Termination date: 20110122 |