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CN201145651Y - Sampling apparatus for measuring plant discharged N2O and 15/14 N isotopic abundance - Google Patents

Sampling apparatus for measuring plant discharged N2O and 15/14 N isotopic abundance Download PDF

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Publication number
CN201145651Y
CN201145651Y CNU2007201852057U CN200720185205U CN201145651Y CN 201145651 Y CN201145651 Y CN 201145651Y CN U2007201852057 U CNU2007201852057 U CN U2007201852057U CN 200720185205 U CN200720185205 U CN 200720185205U CN 201145651 Y CN201145651 Y CN 201145651Y
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CN
China
Prior art keywords
gas
parts
collecting device
isotopic abundance
plant
Prior art date
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Expired - Fee Related
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CNU2007201852057U
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Chinese (zh)
Inventor
徐慧
张颖
李慧
倪志龙
史荣久
杨伟超
韩斯琴
陈冠雄
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Institute of Applied Ecology of CAS
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Institute of Applied Ecology of CAS
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Priority to CNU2007201852057U priority Critical patent/CN201145651Y/en
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Abstract

The utility model relates to the trace gas stable isotope analysis technical field, in particular to a sample collecting device for measuring the <15/14>N isotope abundance of N2 discharged by a plant. The gas collecting device comprises a part which has a variable shape and volume during the process of gas pumping, and a gas collector; the plant to be tested is arranged in the closed part; a gas sampling port is arranged on the part, and the gas collector is hermetically connected with the part through the gas sampling port. The collecting device provided by the utility model can be used to quickly and correctly measure the <15/14> isotope abundance of N2 discharged by the plant system.

Description

Measure plant discharging N 2O's 15/14The sample collecting apparatus of N isotopic abundance
Technical field
The utility model relates to the stable isotope analysis technical field of trace gas, specifically is a kind of mensuration plant discharging N 2O's 15/14The sample collecting apparatus of N isotopic abundance.
Background technology
The main cause of global warming is that atmosphere percent of greenhouse gases concentration rises.N 2O comes tertiary important greenhouse gases, and it is CO that its unimolecule heats potentiality 2296 times, the contribution of global warming is accounted for whole greenhouse gases total contribution 7%, it also participates in destroying stratospheric ozone and strengthens UV radiation to the face of land.N in the present atmosphere 2The concentration of O is about 310ppbv, and annual growth is 0.25%, this means present N 2The increase of O concentration will produce long-range influence to Global climate change.
To soil-atmosphere interface N 2The closed enclosure law technology is generally adopted in the research of O exchange, and the gaseous sample that collects calculates N by the gas chromatographic technique analysis 2The O emission flux.At present, measure the N of plant 2The O drainage rate mainly adopts closed separation method, mainly is the immutable glass box of shape, and plant is contained in wherein, regathers gaseous sample in it.
Stable isotope ratios mass-spectrometric technique (IRMS) is a kind of "dead", high-sensitivity analysis method, can be used to analyze N 2The δ value of O, promptly 15N/ 14N.N 2The δ value of O can be used for studying N 2O emission source or production process.
But, when utilizing closed separation method to collect gaseous sample to be determined, owing to contained a certain amount of N in the background air in the gaseous sample of gathering 2O, and from the N of plant or soil 2O may only account for N in the sample 2The very fraction of O, therefore, the δ value that this class sample is measured by isotopic ratio mass spectrum (IRMS) is not the N that determined object-plant emitted just 2The δ value of O, thus a kind of new method must be sought, to eliminate original N in the air 2O is to the influence of measurement result.
Simultaneously, owing to measuring plant discharging N with stable isotope ratio mass spectrometer 2O's 15/14During the N isotopic abundance, generally need the N in the gaseous sample 2More than the O concentration 300ppm, more than the sample volume 100ml.And the N of plant 2The O discharge capacity is often very little, thereby, usually can't measure the sample that is obtained with the stable isotope ratios mass spectrometer.
Because above-mentioned reason can not be measured plant origin N with current techniques 2The δ value of O.
The utility model content
The object of the present invention is to provide a kind of all variable mensuration plant discharging N of shape and volume that utilizes 2O's 15/14The sample collecting apparatus of N isotopic abundance.
For achieving the above object, the technical scheme that the utility model adopted is:
Harvester: be included in the parts 1 and the gas collecting device 2 of shape in the pumping process, variable volume, treat that measuring plants 3 is placed in the airtight parts 1; Have gas sampling mouth 3 on parts 1, gas collecting device 2 is tightly connected by gas sampling mouth 3 and parts 1.
Described parts 1 are provided with seal 4, treat that measuring plants 3 is placed in the parts 1 by seal 4, and seal 4 seals at the gas collecting state.Described parts 1 can be printing opacity, air-locked polybag or teflon bag.Described gas collecting device 2 can be impermeability needle tubing or gas collecting bag.
The advantage that the utility model had:
1. the utility model utilizes all variable transparent plastic bag of shape and volume as sampling apparatus, and volume is dwindled as far as possible, that is to say, even the N that newly emits 2O amount is smaller, its concentration height of also can trying one's best.In the past at the N of plant 2Can not measure its N under the low situation of O discharge capacity 2The δ value of O adopts method and apparatus of the present invention, then can measure.
2. the utility model gas collecting device is parts and the gas collecting device that is included in shape in the pumping process, variable volume, treats that measuring plants is placed in the airtight parts; Have the gas sampling mouth on parts, the gas collecting device is tightly connected by gas sampling mouth and parts.Adopt harvester of the present invention can measure plant botanical system discharging N convenient, exactly 2O's 15/14N stable isotope abundance.
Description of drawings
Fig. 1 does not replace the preceding synoptic diagram of artificial air for the utility model determinator.
Fig. 2 does not replace synoptic diagram behind the artificial air for the utility model determinator.
Embodiment:
Be included in the parts 1 and the gas collecting device 2 of shape in the pumping process, variable volume, treat that measuring plants 3 is placed in the airtight parts 1; Have gas sampling mouth 3 on parts 1, gas collecting device 2 is tightly connected by gas sampling mouth 3 and parts 1.Described parts 1 are provided with seal 4, treat that measuring plants 3 is placed in the parts 1 by seal 4, and seal 4 seals at the gas collecting state.Described parts 1 can be transparent polybag or teflon bag.Described gas collecting device 2 can be needle tubing.
Choosing soybean is research object, measures the N of soybean discharging season at soybeans they grow 2The δ of O 15The N value.Will be in the season of growth of soybean, the fresh sample of gathering soybean plant strain respectively is as plant sample to be determined, puts into the polybag 1 of determinator by seal 4, then with seal 4 in the sealing of gas collecting state, with not containing N 2The O artificial air replaces 3 times by the sample collecting mouth of offering on the determinator, pack into syringe extracting gases sample from thieff hatch after 40-60 minute in the 200-500ml sampler bag closing the sample collecting mouth, treat that the isotopic ratio mass spectrum (IRMS) analysis is with the δ that measures in the gaseous sample 15The N value.

Claims (4)

1. measure plant discharging N for one kind 2O's 15/14The sample collecting apparatus of N isotopic abundance is characterized in that: be included in the parts (1) and the gas collecting device (2) of shape in the pumping process, variable volume, treat that measuring plants (3) is placed in the airtight parts (1); Have gas sampling mouth (3) on parts (1), gas collecting device (2) is tightly connected by gas sampling mouth (3) and parts (1).
2. by the described mensuration plant discharging of claim 1 N 2O's 15/14The sample collecting apparatus of N isotopic abundance is characterized in that: described parts (1) are provided with seal (4), treat that measuring plants (3) is placed in the parts (1) by seal (4), and seal (4) seals at the gas collecting state.
3. by the described mensuration plant discharging of claim 1 N 2O's 15/14The sample collecting apparatus of N isotopic abundance is characterized in that: described parts (1) can be printing opacity, air-locked polybag or teflon bag.
4. by the described mensuration plant discharging of claim 1 N 2O's 15/14The sample collecting apparatus of N isotopic abundance is characterized in that: described gas collecting device (2) can be impermeability needle tubing or gas collecting bag.
CNU2007201852057U 2007-12-26 2007-12-26 Sampling apparatus for measuring plant discharged N2O and 15/14 N isotopic abundance Expired - Fee Related CN201145651Y (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CNU2007201852057U CN201145651Y (en) 2007-12-26 2007-12-26 Sampling apparatus for measuring plant discharged N2O and 15/14 N isotopic abundance

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CNU2007201852057U CN201145651Y (en) 2007-12-26 2007-12-26 Sampling apparatus for measuring plant discharged N2O and 15/14 N isotopic abundance

Publications (1)

Publication Number Publication Date
CN201145651Y true CN201145651Y (en) 2008-11-05

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Country Status (1)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104913956A (en) * 2015-06-10 2015-09-16 广西大学 Device for collecting carbon isotopes of aldehyde ketone compound discharged by plants
CN106248444A (en) * 2016-08-26 2016-12-21 吕文强 Soil respiration CO2the collection of gas and carbon stable isotope value method of testing thereof

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104913956A (en) * 2015-06-10 2015-09-16 广西大学 Device for collecting carbon isotopes of aldehyde ketone compound discharged by plants
CN106248444A (en) * 2016-08-26 2016-12-21 吕文强 Soil respiration CO2the collection of gas and carbon stable isotope value method of testing thereof

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Granted publication date: 20081105

Termination date: 20100126