CN201136864Y - Cell program freezing instrument with rapid cooling and rewarming functions - Google Patents

Abstract

The utility model relates to a cell procedure refrigeration appearance with rapid cooling rewarming, including the temperature control pipe and the temperature measurement and control device on arranging the sample frame of low-temperature box in, its characteristics are: the temperature control tube consists of a copper tube, an insulating layer and an electric heating wire, wherein the copper tube is sleeved on the sample tube, the insulating layer covers the copper tube, and the electric heating wire is wound on the insulating layer; the temperature measurement and control device consists of a temperature data collector and a temperature control device, wherein the temperature data collector comprises a temperature control pipe and a thermocouple, the thermocouple is inserted into the temperature control pipe, and a thermoelectric signal of the thermocouple controls the electric heating wire after passing through the temperature control device. The device can solve the technical problems of biological cell cooling and rewarming control, has the characteristics of high uniformity of sample temperature, effective rewarming control device, timely and rapid response, suitability for cooling in various cold source environments, small size, low manufacturing cost, convenience in operation and the like, and can be widely used for program cooling and rewarming of various biological materials for low-temperature storage.

Description

Programmed cell freezer with rapid cooling and rewarming

technical field

The utility model relates to a temperature control device for biological materials, in particular to a device for biological cell freezing and cryopreservation which adopts program cooling and rewarming.

Background technique

In industries such as medicine, biology, agriculture, animal husbandry and fishery, some biological materials need to be stored at low temperature. Such as cryopreservation of biological materials such as cornea, blood, bone marrow, semen, embryos, etc. With the development of transplantation research, the demand for various cells is increasing, and effective long-term storage of cells is urgently needed to promote the role of transplantation in the clinic. Low temperature preservation is an important method for preserving various cells at present. In order to meet the requirements of low temperature preservation for the cooling process, there are various cooling methods. At present, there is a two-step cooling method using a refrigerator. First, put the sample to be preserved in ice water at 0°C for a period of time, and then put it in an ordinary refrigerator at -20°C and a low-temperature refrigerator at -80°C for storage. After a period of time, the sample was finally put into liquid nitrogen. The advantage of this method is that the equipment is simple and the operation is convenient, but the cooling rate of the sample cannot be controlled, let alone a complicated cooling program. When cryopreserving various biological materials, different materials require different cooling programs. Some complex cells also require the cooling program to be divided into multiple stages, and the key equipment to realize the complex cooling program is the programmed cooling device. The current program cooling instruments can be roughly divided into two categories, liquid nitrogen jet cooling instruments and lifting cooling instruments, each with its own characteristics. The structure of the liquid nitrogen jet cooling instrument is complex and expensive, and its low-temperature solenoid valve is prone to frost under low temperature conditions, causing the valve to be closed tightly or unable to operate; and the liquid nitrogen consumption is relatively large. Refilling the liquid nitrogen container with liquid nitrogen will interfere with or destroy the cooling procedure. Although the lifting type cooling instrument has a simple structure, since the cooling and rewarming are carried out by using the temperature gradient in the gas phase region in the neck tube of the liquid nitrogen container, it is difficult to control the uniformity of the temperature for the cooling test of the slender sample tube. The above several cooling methods do not have effective rewarming control.

Contents of the invention

The utility model is to provide a cell freezing device, and at the same time solve the technical problem of cooling and rewarming control of biological cells.

In order to achieve the above object, the technical scheme of the utility model is: a cell freezing device, including a temperature control tube and a temperature measurement and control device placed on the sample rack of the cryogenic box, and its characteristics are: the temperature control tube is made of copper tube, insulating layer , composed of electric heating wire, in which the copper tube is sleeved on the sample tube, and the copper tube is covered with an insulating layer, and the electric heating wire is wound on the insulating layer; the temperature measurement and control device is composed of a temperature data collector and a temperature control device, in which the temperature The data collector includes a temperature control tube and a thermocouple. The thermocouple is inserted in the temperature control tube, and the thermoelectric signal of the thermocouple passes through the temperature control device to control the electric heating wire.

The temperature control device includes an amplifier, an A/D converter, a microcomputer, a D/A converter, and a current driver. The thermoelectric signal of the thermocouple is input into the microcomputer through the amplifier and the A/D converter. After the program is compared and processed, the control signal is output, and the electric heating wire is controlled by the D/A converter and the current driver; the electric heating wire of the temperature control tube is covered with a protective layer.

The beneficial effects of the utility model are:

1. Since the cold or heat is directly transferred to the sample by the solid metal, the heat transfer is enhanced, and the temperature difference between the measured temperature and the actual temperature of the sample caused by thermal inertia is reduced;

2. The response is timely and rapid, and the temperature of the sample can be accurately and quickly controlled;

3. The temperature uniformity of the sample is high when heating and cooling;

4. Effective rewarming control device, without taking out the sample, it can be directly rewarmed in the device;

5. Suitable for cooling in various cold source environments;

6. It is easy to operate. The user puts the sample that needs to be stored at low temperature directly into the device, and then the program can cool down. The temperature control and data processing can be completed through the pre-programmed program;

7. The structure is simple, the device is small and exquisite, and the cost is low.

Description of drawings

Fig. 1 is the overall structural representation of the utility model;

Fig. 2 is a schematic perspective view of the structure of the temperature control tube.

Detailed ways

The utility model is described in detail below in conjunction with accompanying drawing and embodiment.

As shown in Figure 1, the cell freezing device of the present utility model comprises a low temperature box 1, an insulating layer 2, a sample rack 3, a sample tube 4, a temperature control tube 5, a thermocouple 6, an amplifier 7, an A/D converter 8, Microcomputer 9, D/A converter 10, current driver 11, copper tube 12, insulating layer 13, electric heating wire 14, protective layer 15.

As shown in Figure 2, the temperature control tube 5 is composed of a copper tube 12, an electric heating wire 14, an insulating layer 13, etc., wherein the copper tube 12 is set on the sample tube 4, and the temperature control tube 5 adopts a copper tube 12 or other materials that are easy to conduct heat. It is made of metal, covered with a layer of insulating material 13, then wound with an electric heating wire 14 on the insulating layer, and finally covered with a protective layer 15 to protect the electric heating wire 14.

The temperature control tube 5 can be fixed by the sample holder 3, and a thermocouple 6 is placed in the reference tube therein to form a temperature measurement and control device.

As shown in Figure 1, the temperature control device is composed of an amplifier 7, an A/D converter 8, a microcomputer 9, a D/A converter 10, and a current driver 11. The lead wire of the thermocouple 6 is connected to the amplifier 7, the A/D converter 8, and the microcomputer 9 in sequence. The sample tubes containing the samples are put into the temperature control tube 5 in sequence and then put into the low temperature box 1 . The signal from the thermocouple 6 is converted into a digital signal by the amplifier 7 and the A/D converter 8, processed by the microcomputer 9 and compared with the preset cooling program, and then passed by the D/A converter 10 and driven by the current driver 11 Determine the electric heating wire 14 power. After fixing the micro-temperature control tube 5 with a shelf, put it into a low-temperature environment, such as a low-temperature refrigerator or a gas phase region of a liquid nitrogen tank. In order to obtain the temperature of the sample, a temperature control tube 5 can be selected as a reference tube, and a thermocouple 6 is placed in the sample to measure the temperature. After the output signal is processed by the microcomputer 9, the electric heating wire is controlled by the temperature control device. 14 power, so as to achieve the purpose of precisely controlling the sample temperature and cooling rate.

The utility model has the characteristics of high sample temperature uniformity, effective re-temperature control device, timely and rapid response, suitable for cooling in various cold source environments, small size, low cost, and convenient operation. It can be widely used in the programmed cooling and rewarming of cryopreservation of various biological materials.

Claims (3)

1. A programmed cell freezer with fast cooling and rewarming, comprising a temperature control tube (5) and a temperature measurement and control device placed on the sample rack (43) of the low temperature box (1), it is characterized in that the temperature control The tube (5) is composed of a copper tube (12), an insulating layer (13), and an electric heating wire (14), wherein the copper tube (12) is sleeved on the sample tube (4), and the copper tube (12) is covered with a layer An insulating layer (13), an electric heating wire (14) is wound on the insulating layer (13); the temperature measurement and control device is composed of a temperature data collector and a temperature control device, wherein the temperature data collector includes a temperature control tube (5), a thermocouple (6), the thermocouple (6) is inserted in the temperature control tube (5), and the thermoelectric signal of the thermocouple (6) controls the electric heating wire (14) after passing through the temperature control device. 2. The programmed cell freezer with fast cooling and rewarming according to claim 1, characterized in that, the temperature control device comprises an amplifier (7), an A/D converter (8), a microcomputer (9), a D /A converter (10), current driver (11), the thermoelectric signal of described thermocouple (6) inputs microcomputer (9) through amplifier (7), A/D converter (8), and microcomputer (9) gathers After the temperature signal is compared with the set cooling program, the control signal is output to control the electric heating wire (14) through the D/A converter (10) and the current driver (11). 3. The cell cryochamber with rapid cooling and rewarming according to claim 1, characterized in that, the electric heating wire (14) of the temperature control tube is covered with a protective layer (15).

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