CN201072428Y - A multifunctional microelectrode chip flow detection cell device - Google Patents

Abstract

The utility model discloses a multifunctional microelectrode chip circulation detection pool device. The upper surface of the lower platen is embedded with an electromagnetic field coil, and guide rail electrodes are respectively provided on both sides, and the guide rail electrodes are respectively electrically connected with the coil leads of the electromagnetic field coil; There are guide rail electrodes on both sides of the pressure plate. The guide rail electrodes are electrically connected to the electrode leads of the microelectrode chip. The upper pressure plate has a fluid inlet and a fluid outlet. Two pipe connectors respectively install the pipes on the fluid inlet and outlet of the upper pressure plate. wherein the microelectrode chip is located below the elastic membrane and above the lower platen, the electromagnetic field coil is located below the microelectrode chip and above the lower platen, and the fluid inlet and outlet fluids are respectively aligned with the two ports of the elastic membrane flow groove. The utility model has the advantages of simple structure, advanced function, convenient installation and cleaning, and can be widely used in the fields of biology, chemistry, life science and the like.

Description

A multifunctional microelectrode chip flow detection cell device

technical field

The utility model relates to a multifunctional microelectrode chip circulation detection pool device.

Background technique

Due to its small physical scale, microfluidic chips can reach micron and nanometer scales, and their excellent detection characteristics are widely used in the fields of biology, chemistry, and life sciences. At present, a microfluidic chip is generally composed of a microelectrode chip and a detection cell. The microelectrode chip and the detection cell are fixedly assembled into a microfluidic chip through a certain technology (chemical combination or physical combination), but the microfluidic chip However, its production and processing are limited by processing equipment and technology, and its performance is greatly affected, such as flow cell blockage, leakage, and difficult cleaning.

Contents of the invention

The purpose of the utility model is to provide a multifunctional microelectrode chip circulation detection pool device.

The technical scheme that the utility model adopts is as follows:

It includes an upper platen, an elastic membrane, a lower platen, a microelectrode chip, an electromagnetic field coil, four rail electrodes, two pipe connectors and two pipes. The upper surface of the lower platen is embedded with an electromagnetic field coil, and the two long sides of the lower platen are respectively equipped with guide rail electrodes, and the two guide rail electrodes are respectively electrically connected to the coil leads of the electromagnetic field coil. There is a flow groove on the end face, guide rail electrodes are respectively provided on the two long sides of the upper platen, and the two guide rail electrodes are electrically connected to the electrode leads of the microelectrode chip, and the upper platen is provided with a fluid inlet and a fluid outlet, and the two pipe connectors are connected by threads Pipelines are respectively installed on the fluid inlet and outlet fluid of the upper platen; the upper platen, the lower platen and the elastic film are fixed by fixing bolts, wherein the microelectrode chip is located below the elastic film and above the lower platen, and the electromagnetic field coil is located on the microelectrode chip Below and above the lower pressing plate, the fluid inlet and outlet are respectively aligned with the two ports of the elastic membrane flow groove.

The utility model has the beneficial effects that: the multifunctional microelectrode chip circulation detection pool device has simple structure, advanced function, convenient installation and cleaning, can be widely used in biology, chemistry, life science and other fields, and can replace complicated MEMS-based Microfluidic chips of (MEMS) technology can be widely used in life sciences, chemical analysis, food safety, environmental monitoring and related scientific fields.

Description of drawings

Fig. 1 is the system schematic diagram of the present utility model;

Fig. 2 is a microelectrode chip diagram of the present utility model;

Fig. 3 is a plan view of the elastic membrane of the present utility model;

Fig. 4 is the A-A sectional view of Fig. 3;

Fig. 5 is a top view of the upper platen of the present utility model;

Fig. 6 is the front view of Fig. 5;

Fig. 7 is a top view of the lower platen of the present invention;

Fig. 8 is a sectional view along A-A of Fig. 7 .

In the figure, 1. upper platen, 2. elastic film, 3. lower platen, 4. microelectrode chip, 5. electromagnetic field coil, 6. 7. pipe connector, 8. 9. 10. 11. rail electrode, 12. 13, pipeline, 14, 15, fixing bolt, 101, 102, 103, 104, fixing hole, 105, fluid inlet, 106, fluid outlet, 201, circulation groove, 202, 203, 204, 205, fixing hole, 301, 302, 303, 304, fixing holes, 401, 402, electrode leads, 501, 502, coil leads.

Detailed ways

Below in conjunction with accompanying drawing and embodiment the utility model is further described.

As shown in Figures 1 to 8, the utility model includes an upper platen 1, an elastic membrane 2, a lower platen 3, a microelectrode chip 4, an electromagnetic field coil 5, four rail electrodes 8, 9, 10, 11, and two pipes connected 6, 7 and two pipes 12, 13; the upper end surface of the lower platen 3 is embedded with an electromagnetic field coil 5, and the two long sides of the lower platen 3 are respectively provided with guide rail electrodes 10, 11, and the two guide rail electrodes 10, 11 are connected to the electromagnetic field respectively. The coil leads 501 and 502 of the coil 5 are electrically connected, the lower end surface of the elastic membrane 2 is embedded with a microelectrode chip 4, the upper end surface of the elastic membrane 2 is opened with a flow groove 201, and the two long sides of the upper platen 1 are respectively provided with rail electrodes 8, 9 , two rail electrodes 8,9 are electrically connected with the electrode lead wires 401,402 of the microelectrode chip 4 respectively, and the upper platen 1 has a fluid inlet 105 and a fluid outlet 106, and the two pipeline connectors 6,7 respectively connect the pipeline 12 through threaded connections. , 13 are installed on the fluid inlet 105 and the outlet fluid 106 of the upper pressing plate 1; The fixing holes 202, 203, 204, 205 on the four corners of the film 2 and the fixing holes 301, 302, 303, 304 on the four corners of the lower pressing plate 3 are fixed with fixing bolts 14 and 15. Wherein the microelectrode chip 4 is located below the elastic membrane 2 and above the lower pressing plate 3, the electromagnetic field coil 5 is located below the microelectrode chip 4 and above the lower pressing plate 3, and the fluid inlet 105 and the outlet fluid 106 are respectively aligned on the elastic membrane 2 Two ports of the flow cell 201.

The microelectrode chip 4 adopts the microelectrode chip of ABTECH Company in the United States. The elastic membrane 2 is made of PDMS material. The included angle between the two pipeline connectors 6, 7 and the normal line is 35°-85°.

The application process of the present utility model is as follows:

1. Push the microelectrode chip flow detection cell device into the existing impedance detection instrument, the guide rail electrodes 8 and 9 on the upper platen 1 are respectively connected to the signal input terminals of the impedance detection instrument, and the guide rail electrodes 10, 9 on the lower platen 3, 11 are respectively connected with the electromagnetic field coil control circuit.

2. Inject the sample to be detected into the pipeline 12, and enter the flow channel 201, and flow to the upper surface of the microelectrode chip 4; the flow channel 201 is perpendicular to the interdigitated electrodes of the microelectrode chip 4; read the impedance signal of the impedance detection instrument Then the impedance characteristic of the sample to be detected can be known.

3. If there are immunoseparation magnetic beads in the specimen to be detected, start the electromagnetic field coil control circuit and generate a magnetic field, so that the immunoseparation magnetic beads gather on the surface of the microelectrode chip 4, and the impedance characteristic of the specimen to be detected can be known by detecting the impedance signal.

4. To clean the electrodes, it is only necessary to disassemble the microelectrode chip flow detection cell device and clean the elastic membrane 2 and the microelectrode chip 4, so that the chip can be reused.

5. By changing the size of the flow groove 201 of the elastic membrane 2, the size of the flow detection cell can be changed.

The above-mentioned specific embodiments are used to explain the utility model, rather than to limit the utility model. Within the spirit of the utility model and the scope of protection of the claims, any modifications and changes made to the utility model fall into the scope of the utility model. scope of protection.

Claims (4)

1. A multifunctional microelectrode chip circulation detection cell device is characterized in that: comprise upper platen (1), elastic film (2), lower platen (3), microelectrode chip (4), electromagnetic field coil (5), Four guide rail electrodes (8, 9, 10, 11), two pipeline connectors (6, 7) and two pipelines (12, 13); the upper end surface of the lower pressure plate (3) is embedded with an electromagnetic field coil (5), The two long sides of the lower pressing plate (3) are respectively provided with guide rail electrodes (10, 11), and the two guide rail electrodes (10, 11) are respectively electrically connected to the coil leads (501, 502) of the electromagnetic field coil (5), and the elastic film ( 2) A microelectrode chip (4) is embedded on the lower end surface, a circulation groove (201) is opened on the upper end surface of the elastic membrane (2), and guide rail electrodes (8, 9) are respectively provided on the two long sides of the upper pressing plate (1). The root rail electrodes (8, 9) are electrically connected to the electrode leads (401, 402) of the microelectrode chip (4) respectively, and the upper platen (1) is provided with a fluid inlet (105) and a fluid outlet (106), and two pipe connectors (6,7) Pipelines (12,13) are respectively installed on the fluid inlet (105) and outlet fluid (106) of the upper platen (1) through threaded connections; the upper platen (1), the lower platen (3) and the elastic film (2) Fix by fixing bolts, wherein the microelectrode chip (4) is located below the elastic film (2) and above the lower platen (3), and the electromagnetic field coil (5) is located below the microelectrode chip (4) and is located on the lower platen (3). Above the pressing plate (3), the fluid inlet (105) and outlet fluid (106) are respectively aligned with the two ports of the flow groove (201) of the elastic membrane (2). 2. A multifunctional microelectrode chip flow detection cell device according to claim 1, characterized in that: said microelectrode chip (4) is a microelectrode chip produced by ABTECH Corporation of the United States. 3. A multifunctional microelectrode chip flow-through detection cell device according to claim 1, characterized in that: the elastic membrane (2) is made of PDMS material. 4. A kind of multifunctional microelectrode chip flow-through detection cell device according to claim 1, characterized in that: the angle between the two pipe connectors (6, 7) and the normal line is 35°- 85 degrees.

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