CN209802988U - Multifunctional spectrum system - Google Patents

Abstract

The utility model discloses a multifunctional spectrum system, which relates to the technical field of analysis and detection. The technical solution is that, according to the order of the optical path, a light source assembly, a first spectrum assembly, a first sample chamber, a second spectrum assembly and a first detector assembly are sequentially provided, wherein a second sample chamber is also provided; the first The second sample chamber is connected to the first spectrum assembly, and the second sample chamber is provided with an exit slit; the first detector assembly is connected to the second spectrum assembly; or, the first detector assembly is connected to the second sample chamber connect. A spectral test method that can realize multiple functions by using one system.

Description

A multifunctional spectroscopic system

technical field

The utility model relates to the technical field of analysis and detection, in particular to a multifunctional spectrum system.

Background technique

Spectral technology is a new analysis and detection technology developed in recent years. It has the advantages of high sensitivity, low detection limit, simple operation, short analysis time, no loss, no pollution and low cost. Now the spectral technology and its application began in the 1960s In recent decades, due to its unique characteristics, spectroscopic technology has been widely used and developed in the fields of chemical industry, wood industry, medicine and health, etc. Now, spectroscopic technology has gradually developed into an important and effective spectrochemical means of analysis.

At present, there are many analysis methods of spectral technology, including fluorescence excitation spectroscopy, fluorescence emission spectroscopy, absorption spectroscopy, fluorescence lifetime spectroscopy, etc. These spectral analysis methods are all implemented by specific spectral systems, but currently Most of the instruments can only realize the function of one or two kinds of spectral analysis methods, which results in the incomplete spectral information of materials obtained by people on one spectroscopic system, and needs to use other spectroscopic instruments to obtain more spectral information, which not only It has caused inconvenience to test analysts and increased the cost of purchasing instruments.

Utility model content

The technical problem to be solved by the utility model is how to implement multiple spectral means on a system to comprehensively detect the properties of materials, and to provide a multifunctional spectral system.

In order to solve the above problems, the utility model proposes the following technical solutions:

A multifunctional spectroscopic system, according to the sequence of optical paths, sequentially provided with a light source assembly, a first spectral assembly, a first sample chamber, a second spectral assembly and a first detector assembly, wherein a second sample chamber is also provided; The second sample chamber is connected to the first spectrum assembly, and the second sample chamber is provided with an exit slit; the first detector assembly is connected to the second spectrum assembly; or, the first detector assembly is connected to the second spectrum assembly. Sample compartment connection.

Its further technical solution is to further include a second detector assembly, the second detector assembly is connected to the second spectrum assembly; or, the second detector assembly is connected to the second sample chamber.

Its further technical solution is to further include a refrigeration device connected to the first sample chamber; or, the refrigeration device to be connected to the second sample chamber.

Its further technical solution is that the refrigerating device is provided with a refrigerating chamber, the refrigerating chamber is provided with a place for placing samples, and the refrigerating chamber is surrounded by transparent quartz plates.

Its further technical solution is that the refrigeration device is a refrigeration device whose refrigeration temperature can reach 10-300K.

Its further technical solution is that the first detector component is a visible light detector or a near-infrared detector; the second detector component is a visible light detector or a near-infrared detector.

Its further technical solution is to further include an extended light source assembly, and the extended light source assembly is connected to the first sample chamber.

Its further technical solution is that the extended light source component is a picosecond laser.

Its further technical solution is that the light source assembly includes a bromine tungsten lamp light source and/or a white laser light source; the white laser light source is a light source that can emit a spectrum with a wavelength of 200-2000nm.

Compared with the prior art, the multifunctional spectrum system provided by the utility model combines the light source component, the first spectrum component, the first sample chamber, the second spectrum component and the first detector component to perform fluorescence excitation spectrum experiments , Fluorescence emission spectrum experiment; and the combination of the light source component, the first spectrum component, the second sample chamber and the first detector component can be used for absorption spectrum experiment. Therefore, only one set of system can realize the spectral testing method with multiple functions.

Description of drawings

In order to more clearly illustrate the technical solutions of the embodiments of the utility model, the following will briefly introduce the accompanying drawings that need to be used in the description of the embodiments. Obviously, the accompanying drawings in the following description are some embodiments of the utility model. For Those of ordinary skill in the art can also obtain other drawings based on these drawings without making creative efforts.

Fig. 1 is a schematic structural diagram of a multifunctional spectroscopic system provided by an embodiment of the present invention.

Reference signs: light source assembly 10, first spectrum assembly 20, first sample chamber 30, first sample placement place 31, second spectrum assembly 40, first detector assembly 50, second sample chamber 60, exit narrow Slit 61, second sample placement place 62, second detector assembly 70, cooling device 80, extended light source assembly 90.

Detailed ways

The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention, and similar component numbers in the drawings represent similar components. Apparently, the embodiments to be described below are only some of the embodiments of the present invention, not all of them. Based on the embodiments of the present utility model, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the scope of protection of the present utility model.

It should be understood that when used in this specification and the appended claims, the terms "comprising" and "comprises" indicate the presence of described features, integers, steps, operations, elements and/or components, but do not exclude one or Presence or addition of multiple other features, integers, steps, operations, elements, components and/or collections thereof.

It should also be understood that the terms used in the description of the embodiments of the present invention are only for the purpose of describing specific embodiments and are not intended to limit the embodiments of the present invention. As used in the description of embodiments of the present invention and the appended claims, unless the context clearly indicates otherwise, the singular forms "a", "an" and "the" are intended to include the plural forms.

Referring to Fig. 1, the embodiment of the utility model proposes a multi-functional spectroscopic system, which is provided with a light source component 10, a first spectroscopic component 20, a first sample chamber 30, a second spectroscopic component 40 and a first detection The device assembly 50, wherein a second sample chamber 60 is also provided; the second sample chamber 60 is connected to the first spectrum assembly 20, and the second sample chamber 60 is provided with an exit slit 61; the first detector The component 50 is connected with the second spectrum component 40 ; or, the first detector component 50 is connected with the second sample chamber 60 .

In a specific implementation, the light source assembly 10 is provided with a light source and at least one focusing lens, and the focusing lens is used for converging the light emitted by the light source.

In some embodiments, the light source assembly 10 is further provided with a half-mirror, and the light emitted by the light source passes through the half-mirror to change the light path, and then converged by the focusing lens to increase the light intensity.

In a specific implementation, the first spectral component 20 is provided with a grating and at least one focusing mirror. The first spectral component receives the light emitted from the light source component 10, and is converged by the focusing mirror to the grating to be divided into the required monochromatic light. The monochromatic light is converged to increase the light intensity of the monochromatic light.

In some embodiments, the first spectrum component 20 is also provided with a half-mirror. The light emitted by the light source component 10 passes through the half-mirror, changes the optical path, and is converged by the focusing mirror to the grating to be divided into the required monochromatic light. , and then the monochromatic light is converged by the focusing mirror, and then emitted to the first sample chamber 30 after increasing the light intensity.

In some embodiments, the direction of the light path can be changed by adding a half-mirror, so that the light path is sent to a preset direction.

In specific implementation, the first sample chamber 30 is provided with a first sample placement place 31 and at least one focusing lens. The light emitted by the spectroscopic component 20 is converged, collimated, and then focused to the first sample placement place 31 through the focusing lens.

In a specific implementation, the second spectroscopic component 40 is provided with at least one focusing lens for converging the fluorescence from the first sample compartment 30 to increase light intensity and improve detection sensitivity. In some embodiments, the optical path can also be changed by adding a half-mirror, so that the optical path reaches the first detector assembly 50 according to a preset track.

Thus, the light source assembly 10, the first spectrum assembly 20, the first sample chamber 30, the second spectrum assembly 40 and the first detector assembly 50 can be used in combination to perform fluorescence excitation spectroscopy experiments and fluorescence emission spectroscopy experiments.

In specific implementation, a second sample compartment 60 is also provided; the second sample compartment 60 is provided with an exit slit 61 and a second sample placement place 62; the first detector assembly 50 is connected to the second sample compartment 60; The light transmitted by the first spectral component 20 turns to the second sample chamber 60, and is decomposed into monochromatic light beams of required wavelengths through the exit slit 61, and is absorbed by the sample at the second sample placement place 62, and then the light is transmitted by the focusing lens To the first detector assembly 50, the light intensity absorbed by the sample at a given wavelength is measured, that is, an absorption spectrum experiment is performed.

It should be noted that the light source assembly 10, the first spectrum assembly 20, the first sample chamber 30, the second spectrum assembly 40 and the second sample chamber 60 can be added with a half mirror, a focusing mirror, and a focusing lens according to the needs. It is used to adjust the direction of the optical path, collimate and focus the light. This embodiment does not specifically limit the number and specifications of the above optical mirrors.

In this embodiment, the first detector assembly 50 is connected to the second spectrum assembly 40; or, the first detector assembly 50 is connected to the second sample chamber 60, and only one set of detector assemblies is used to detect the spectrum, which can reduce the The cost.

The light source assembly 10, the first spectrum assembly 20, the second sample chamber 60 and the first detector assembly 50 are used in combination to perform absorption spectroscopy experiments.

Therefore, only one set of system can realize the spectral testing method with multiple functions.

In some embodiments, such as this embodiment, the multifunctional spectroscopy system further includes a second detector assembly 70, which is connected to the second spectroscopy assembly 50; or, the second detector assembly 70 is connected with the second sample chamber 60.

In a specific embodiment, the first detector assembly 50 and the second detector assembly 70 are detachable, and the two can exchange positions. Both the first detector assembly 50 and the second detector assembly 70 are provided with detectors for detecting light intensity. Appropriate detectors can be selected according to the range of fluorescence intensity emitted by the sample, which is not specifically limited in this embodiment.

In this embodiment, a set of detector components is added, and each corresponding set of detector components is used for detection without repeated disassembly and assembly, which is simple and convenient.

In some embodiments, the multifunctional spectroscopic system further includes a refrigeration device 80 connected to the first sample compartment 30 ; or, the refrigeration device 80 is connected to the second sample compartment 60 .

The refrigeration device 80 is a turnable liquid nitrogen refrigerator, which can be connected to the required sample chamber according to the detection needs, so as to assist in the fluorescence excitation spectrum temperature change experiment, the fluorescence emission spectrum temperature change experiment, the absorption spectrum temperature change experiment, and the fluorescence lifetime Spectral variable temperature experiment.

In some embodiments, the refrigerating device 80 is provided with a refrigerating cavity, which can place samples, and the refrigerating cavity is surrounded by a transparent quartz plate.

When conducting variable temperature experiments, move the cooling chamber of the liquid nitrogen refrigerator to the first sample placement place 31 or the second sample placement place 62, and place the samples in the cooling chamber. In order to ensure accurate detection, the cooling chamber is made of 5 pieces of transparent Surrounded by quartz sheets.

In some embodiments, the refrigeration device is a refrigeration device whose refrigeration temperature can reach 10-300K.

In some embodiments, the first detector assembly 50 is a visible light detector or a near-infrared detector; the second detector assembly 70 is a visible light detector or a near-infrared detector. Wherein, the detection range of the visible light detector is 200-900nm, and the detection range of the near-infrared detector is 800-2600nm.

In some embodiments, the near infrared detector is an InGaAs detector.

In some embodiments, the multifunctional spectroscopy system further includes an extended light source assembly 90 connected to the first sample compartment 30 .

In some embodiments, the extended light source assembly 90 is a 375nm picosecond laser.

When performing fluorescence lifetime spectrum detection, the laser light emitted by the extended light source assembly 90 is irradiated on the first sample placement place 31, the sample is excited to generate fluorescence, and the fluorescence enters the second spectrum assembly 40, and the grating selection spectrum is set at the most emitted fluorescence spectrum. The strength is detected by the focusing mirror to the first detector assembly 50, and the first detector assembly 50 is connected to the data acquisition card to collect the fluorescence decay lifetime spectrum curve.

In some embodiments, the light source assembly 10 includes a bromine tungsten light source and/or a white laser light source; the white laser light source is a light source that can emit a spectrum with a wavelength of 200-2000nm. The white light laser light source has a relatively smooth spectral curve in the full spectral range, and has a higher optical power density than the usual xenon lamp and bromine tungsten light source, and can obtain a better spectral signal-to-noise ratio.

The multifunctional spectrum system provided in this embodiment can be used for fluorescence excitation spectrum experiments, fluorescence emission spectrum experiments, absorption spectrum experiments, and fluorescence lifetime spectroscopy experiments. By adding a refrigeration device whose refrigeration temperature can reach 10K to 300K, it can assist in the fluorescence excitation spectrum temperature change experiment, the fluorescence emission spectrum temperature change experiment, the absorption spectrum temperature change experiment, and the fluorescence lifetime spectrum temperature change experiment.

The spectral testing means provided by the multifunctional spectral system provided in this embodiment are respectively:

Fluorescence excitation spectrum: Let the excitation light of different wavelengths excite the fluorescent substance to make it fluoresce, and let the fluorescence irradiate the detector at a fixed emission wavelength, and then use the wavelength of the excitation light as the abscissa and the fluorescence intensity as the ordinate to draw The figure is the fluorescence excitation spectrum.

In a specific implementation, a specific fluorescence emission wavelength is fixedly selected at the second spectrum component 40 , and the excitation wavelength is converted by rotating the grating in the second spectrum component 40 .

Fluorescence emission spectrum: Keep the wavelength and intensity of the excitation light unchanged, and let the fluorescence emitted by the fluorescent substance irradiate the detector through the emission monochromator, that is, scan, take the fluorescence wavelength as the abscissa, and take the fluorescence intensity as the Plotting the ordinate is the fluorescence emission spectrum.

In the specific implementation, the bromine tungsten lamp light source or the white light laser light source enters the first spectrum component 20, and a specific excitation wavelength is selected through grating splitting. On the sample in the sample chamber 30 , the fluorescence generated by the sample is collected through the lens into the second spectrum component 40 , and finally reaches the first detector component 50 .

Absorption Spectroscopy: An analysis method based on measuring the light intensity absorbed by a sample at a given wavelength, usually called spectrophotometry.

In the specific implementation, the bromine tungsten lamp or white light laser light source enters the first spectrum component 20, selects specific output monochromatic light through grating spectroscopic separation, the monochromatic light becomes parallel light after being collimated, and passes through the sample in the second sample chamber 60 Converge to the second detector assembly 70 .

Fluorescence lifetime spectroscopy: When a substance is excited by a beam of laser light, the molecules of the substance absorb energy and transition from the ground state to an excited state, and then fluoresce back to the ground state in the form of a radiative transition. When the excitation light is removed, the time required for the fluorescence intensity of the molecule to drop to 1/e of the maximum fluorescence intensity I0 at the time of excitation is called the fluorescence lifetime, which is usually represented by τ, and the measured fluorescence lifetime data is the fluorescence lifetime spectrum.

In a specific implementation, an extended laser is used to irradiate the sample in the first sample chamber 30 , and the fluorescence emitted by the sample passes through the second spectrum component 40 and enters the first detector component 50 .

In addition, by introducing a refrigeration device, it can assist in the fluorescence excitation spectrum temperature change experiment, the fluorescence emission spectrum temperature change experiment, the absorption spectrum temperature change experiment, and the fluorescence lifetime spectrum temperature change experiment.

In the foregoing embodiments, the descriptions of each embodiment have their own emphases, and for parts not described in detail in a certain embodiment, reference may be made to relevant descriptions of other embodiments.

The above is a specific embodiment of the present utility model, but the scope of protection of the present utility model is not limited thereto. Any skilled person familiar with the technical field can easily think of various other methods within the technical scope disclosed by the utility model. If there are any effective modifications or replacements, these modifications or replacements should be covered within the protection scope of the present utility model. Therefore, the protection scope of the present utility model should be based on the protection scope of the claims.

Claims (9)

1. A multifunctional spectroscopic system, characterized in that, according to the light path sequence, a light source assembly, a first spectral assembly, a first sample chamber, a second spectral assembly and a first detector assembly are successively provided, wherein, a The second sample compartment; the second sample compartment is connected to the first spectrum component, and the second sample compartment is provided with an exit slit; the first detector component is connected to the second spectrum component; or, the first The detector assembly is connected to the second sample compartment. 2. The multifunctional spectroscopic system according to claim 1, further comprising a second detector assembly, the second detector assembly is connected with the second spectrum assembly; or, the second detector assembly is connected with the second spectrum assembly The second sample compartment is connected. 3. The multifunctional spectroscopic system according to claim 1 or 2, further comprising a refrigeration device connected to the first sample chamber; or, the refrigeration device connected to the second sample chamber. 4. The multifunctional spectroscopic system according to claim 3, wherein the refrigeration device is provided with a refrigeration chamber, the refrigeration chamber can place samples, and the refrigeration chamber is surrounded by transparent quartz plates. 5. The multifunctional spectroscopic system according to claim 4, wherein the refrigeration device is a refrigeration device whose refrigeration temperature can reach 10-300K. 6. The multifunctional spectroscopy system according to claim 2, wherein the first detector assembly is a visible light detector or a near-infrared detector; the second detector assembly is a visible light detector or a near-infrared detector device. 7. The multifunctional spectroscopic system according to claim 1, further comprising an extended light source assembly connected to the first sample chamber. 8. The multifunctional spectroscopic system according to claim 7, wherein the extended light source component is a picosecond laser. 9. The multifunctional spectrum system according to claim 1, wherein the light source assembly comprises a bromine tungsten light source and/or a white laser light source; the white laser light source is a light source that can emit a spectrum with a wavelength of 200-2000nm .