CN1965885B - Application of chickpea total flavonoids in the preparation of drugs for treating diabetes - Google Patents
Application of chickpea total flavonoids in the preparation of drugs for treating diabetes Download PDFInfo
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Abstract
本发明涉及鹰嘴豆总黄酮在制备治疗糖尿病药物中的应用。其制备方法如下：鹰嘴豆粉经石油醚脱脂，干燥后用乙醇提取，得到的浸膏用热水溶解，调节pH值至9～11，离心，取上清液，将所得上清液上大孔树脂柱分离，以20％～60％乙醇水溶液梯度洗脱，收集黄酮含量高的部分，蒸发溶剂，得鹰嘴豆总黄酮，黄酮含量大于50％。本发明提取工艺简便，成本低廉，经过纯化的总黄酮含量大于50％，可用于制备治疗糖尿病的药物。The invention relates to the application of chickpea total flavonoids in the preparation of medicines for treating diabetes. The preparation method is as follows: the chickpea powder is degreased with petroleum ether, extracted with ethanol after drying, the obtained extract is dissolved in hot water, the pH value is adjusted to 9-11, centrifuged, and the supernatant is taken. Separation on a macroporous resin column, gradient elution with 20%-60% ethanol aqueous solution, collecting the part with high flavonoid content, evaporating the solvent, and obtaining the total flavonoids of chickpea, the flavonoid content is greater than 50%. The extraction process of the invention is simple and low in cost, the content of the purified total flavonoids is greater than 50%, and can be used for preparing medicines for treating diabetes.
Description
Technical field
The present invention relates to total flavones of chickpea and extracting method thereof and the application in medicine, specifically, is total flavones of chickpea and extracting method thereof and the application in preparation treatment diabetes medicament.
Background technology
Diabetes are metabolic diseases of a kind of multi-pathogenesis, and characteristics are chronic hyperglycemias, follow because of insulin secretion and (or) effect the defective sugar, fat and the protein metabolism disorder that cause.Along with the prolongation of ill time of diabetes, the intravital metabolism disorder of body is controlled well as can not get, will cause losing one's sight, multiple complications such as cardiovascular and cerebrovascular disease, renal failure, neuropathy, stupor, in addition dead.With regard to sickness rate, China's actual trouble diabetic number has reached more than 4,000 ten thousand, and sickness rate increases just with surprising rapidity, the whole nation newly-increased diabetics every day has more than 3000, and diabetes are the Chronic Non-Communicable Diseasess that become the 3rd serious threat human health after the cardiovascular and cerebrovascular disease tumor.
The medicine of the diabetes of China's treatment at present accounts for 73.68% based on chemical medicine; Chinese patent medicine is auxilliary, accounts for 24.7%.The chemistry medicine has insulin, sulphanylureas and biguanides, alpha-glucosidase inhibitor, regulator, euglycemic agent etc. before the meal.Though effect is obvious, the side effect such as can causing drug resistance, Drug resistance and lesions of liver and kidney of taking medicine for a long time.And the flavones ingredient toxic and side effects in the Chinese medicine is little, and have blood fat reducing simultaneously, improve effects such as immunity, endocrine regulation, diabetic complication is had prevention and therapeutical effect, thereby the natural drug of the treatment diabetes of excavation better efficacy has great importance and vast market prospect from medicinal plants.
Chickpea is second largest in the world beans, is the Xinjiang natural plant resource, is widely used in treating diseases such as diabetes, hyperlipemia, dyspepsia, bronchitis according to " Uigurs medicine will " record.Studies show that the significantly blood fat reducing of its isoflavone, but its hypoglycemic activity still there is not research.At present, the thick product flavones content of chickpea alcohol extraction thing is low, no medical value.
Summary of the invention
The new purposes that the purpose of this invention is to provide total flavones of chickpea, i.e. application in preparation treatment diabetes medicament.
Another object of the present invention provides total flavones of chickpea and extracting method thereof.
Total flavones of chickpea mainly contains Biochanin A, formoononetin, genistein and biochanin C, and its weight percentage is greater than 50%; The extracting method of total flavones of chickpea: the olecranon Semen Glycines powder is through defat with petroleum ether, dry back ethanol extraction, the extractum that obtains is used the hot water dissolving, regulates pH value to 9～11, and is centrifugal, get supernatant, macroporous resin column on the gained supernatant is separated,, collect the high part of flavones content with 20%～60% ethanol water gradient elution, evaporating solvent gets total flavones of chickpea.
The part that described collection flavones content is high is 40%～60% eluent; The macroporous resin model is D-101, DS-401 or AB-8 type.
The total flavones of chickpea that the present invention obtains can be used for preparing the medicine for the treatment of diabetes, comprises the medicine of preparation treatment type i diabetes and type ii diabetes.
Aspect pharmacy, can adopt conventional dosage form, as tablet, capsule, granule, powder, pill or slow releasing preparation etc.
The inventor finds that total flavones of chickpea hypoglycemic activity and metformin are suitable, illustrate that the total flavones of chickpea that extracts from chickpea can be used for preparing the medicine for the treatment of diabetes.
The effect experiment of hypoglycemic activity:
1. acute toxicity testing
The Kunming mouse acute toxicity test shows, with the total flavones of chickpea (content is 50.5%) that extracts, during single administration, does not measure LD50, and recording its maximum tolerated dose is the 25g total flavones of chickpea.
2. the hypoglycemic activity of the diabetic mice that alloxan is caused
The NIH mice, tail vein injection alloxan (80mg/kg) is measured serum level of glucose behind the 72h, select blood glucose value to supply examination the above person of 16mmol/L.Be divided into alloxan group, metformin (100mg/kg) group, the high, medium and low dosage of total flavones of chickpea (250,100,50mg/kg) for the examination mice, and establish the normal control group.Every day, gastric infusion was 1 time, and normal control group and alloxan group give the equal-volume distilled water, continuously 7d.1h after the last administration gets blood from the mouse orbit venous plexus, measures blood sugar level.The result shows that each dosage group of total flavones of chickpea all can significantly reduce the blood sugar level of alloxan diabetes mice; The hypoglycemic activity of heavy dose of (250mg/kg) group is similar to the effect of metformin (100mg/kg) group.
Advantage of the present invention: extraction process of the present invention is easy, and is with low cost; The total flavones of chickpea that extracts from chickpea is the hypoglycemic effective ingredient of chickpea, and the general flavone content of extraction can be used for preparing the medicine for the treatment of diabetes greater than 50%.
The specific embodiment
Embodiment 1:
Step 1: will be crushed to 0.5～5mm olecranon Semen Glycines powder 200g and place round-bottomed flask, and add the 1000ml petroleum ether, and be heated to little backflow 1h that boils, sucking filtration, collection olecranon Semen Glycines powder add 1000ml petroleum ether backflow 1h, altogether triplicate once more.With the dry adding of gained defat chickpea dried bean noodles 2400ml 80% alcoholic solution, reflux 3 times down at 65 ℃, each 3 hours, filter, rotary evaporation reclaims ethanol, obtains the dark brown thick paste.
Step 2: the gained thick paste is used the hot water dissolving, regulate pH value to 10,, get supernatant with the gained solution centrifugal.With D101 macroporous resin column on the gained solution, with 20%～60% ethanol water gradient elution, collect 60% eluent, rotary evaporation gets yellowish green powder, and this sample detects through HPLC, and flavones content is 54.3%.
Embodiment 2:
Step 1: will be crushed to 0.5～5mm olecranon Semen Glycines powder 200g and place round-bottomed flask, and add the 1000ml petroleum ether, and be heated to little backflow 1h that boils, sucking filtration is collected the olecranon Semen Glycines powder, adds 1000ml petroleum ether backflow 1h once more, altogether triplicate.Gained defat chickpea dried bean noodles is dry, add 2400ml 80% alcoholic solution, reflux 3 times down at 70 ℃, each 3 hours, filter, rotary evaporation reclaims ethanol, obtains the dark brown thick paste.
Step 2: the gained thick paste is used the hot water dissolving, regulate pH value to 10,, get supernatant with the gained solution centrifugal.With DS-401 macroporous resin column on the gained solution, with 20%～60% ethanol water gradient elution, collect 50% eluent, rotary evaporation gets yellowish green powder, and this sample detects through HPLC, and flavones content is 53.2%.
Claims (7)
1. total flavones of chickpea, it is characterized in that: mainly contain Biochanin A, formoononetin, genistein and biochanin C, its weight percentage is greater than 50%;
The extracting method of total flavones of chickpea: the olecranon Semen Glycines powder is through defat with petroleum ether, dry back ethanol extraction, the extractum that obtains is used the hot water dissolving, regulates pH value to 9～11, and is centrifugal, get supernatant, macroporous resin column on the gained supernatant is separated,, collect 40%～60% ethanol water eluting liquid with 20%～60% ethanol water gradient elution, evaporating solvent gets total flavones of chickpea.
2. the extracting method of the described total flavones of chickpea of claim 1 is characterized in that, the olecranon Semen Glycines powder is through defat with petroleum ether, dry back ethanol extraction, the extractum that obtains is used the hot water dissolving, regulates pH value to 9～11, and is centrifugal, get supernatant, macroporous resin column on the gained supernatant is separated,, collect 40%～60% ethanol water eluting liquid with 20%～60% ethanol water gradient elution, evaporating solvent gets total flavones of chickpea.
3. the extracting method of total flavones of chickpea according to claim 2 is characterized in that the macroporous resin model is D-101, DS-401 or AB-8 type.
4. the application of the described total flavones of chickpea of claim 1 in preparation treatment diabetes medicament.
5. the application of the described total flavones of chickpea of claim 1 in preparation treatment type i diabetes medicine.
6. the application of the described total flavones of chickpea of claim 1 in preparation treatment type ii diabetes medicine.
7. according to one of any described application of claim 4 to 6, be characterised in that the total flavones of chickpea extract is prepared into tablet, capsule, granule, powder, pill or slow releasing preparation.
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| CN2006101048535A CN1965885B (en) | 2006-11-06 | 2006-11-06 | Application of chickpea total flavonoids in the preparation of drugs for treating diabetes |
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| CN2006101048535A CN1965885B (en) | 2006-11-06 | 2006-11-06 | Application of chickpea total flavonoids in the preparation of drugs for treating diabetes |
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| CN1965885A CN1965885A (en) | 2007-05-23 |
| CN1965885B true CN1965885B (en) | 2010-09-08 |
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Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101199581B (en) * | 2007-11-05 | 2012-07-04 | 中国科学院新疆理化技术研究所 | Chick-pea embryo and bean sprout valid part, preparing method and application thereof |
| CN101480421B (en) * | 2008-01-11 | 2012-02-22 | 新疆维吾尔自治区药物研究所 | Application of chickpea lipid in preparing medicament for treating diabetes or health-care food |
| CN101238909B (en) * | 2008-03-06 | 2011-04-13 | 中国科学院新疆理化技术研究所 | Chickpea bean sprout juice and preparation method and application |
| CN101238904B (en) * | 2008-03-20 | 2011-04-13 | 中国科学院新疆理化技术研究所 | Chickpea bean sprout juice tea and preparation method and application |
| CN101757640B (en) * | 2009-06-09 | 2011-08-31 | 东华大学 | Preparation of clathrate compound containing isoflavone and cyclodextrin of chickpea |
| CN102000138B (en) * | 2010-10-28 | 2012-04-18 | 中国科学院新疆理化技术研究所 | Method for purifying effective ingredients of cicer ariethnum L. bean sprouts |
| CN103285070A (en) * | 2013-06-03 | 2013-09-11 | 中国科学院新疆理化技术研究所 | Preparation method and application of chickpea sprout extract dispersible tablet |
| CN106187973B (en) * | 2016-07-15 | 2018-08-10 | 北京中医药大学 | The composition and preparation method and medical application of a kind of polyisocyanate chromocor derivative |
| CN106220602A (en) * | 2016-07-20 | 2016-12-14 | 陕西嘉禾生物科技股份有限公司 | A kind of synthetic method of Biochanin A |
| CN109568252B (en) * | 2018-11-20 | 2020-11-17 | 张明 | Preparation method of biochanin A solution, atomized liquid and taking method |
| CN111560406B (en) * | 2020-04-27 | 2023-05-16 | 黑龙江大学 | A method for producing flavonoids by Bacillus natto fermenting vegetable soybeans and other foreign products |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1264290A (en) * | 1997-07-25 | 2000-08-23 | 尤尼利弗公司 | skin care composition |
| CN1830464A (en) * | 2005-03-07 | 2006-09-13 | 骏神生物医学（上海）有限公司 | Application of olecranone bean extraction in preparation of food for preventing and treating obesity and type II diabetes |
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2006
- 2006-11-06 CN CN2006101048535A patent/CN1965885B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1264290A (en) * | 1997-07-25 | 2000-08-23 | 尤尼利弗公司 | skin care composition |
| CN1830464A (en) * | 2005-03-07 | 2006-09-13 | 骏神生物医学（上海）有限公司 | Application of olecranone bean extraction in preparation of food for preventing and treating obesity and type II diabetes |
Non-Patent Citations (3)
| Title |
|---|
| 何桂香.鹰嘴豆总异黄酮的提取及其对小鼠高脂血症的预防作用研究.中国优秀硕士学位论文全文数据库 医药卫生科技辑 3.2006,(3),第4、6页. * |
| 侯水薇等.鹰嘴豆对小鼠血糖及血脂的影响.新疆师范大学学报 自然科学版24 4.2005,24(4),第62-64页. * |
| 梁少玲等.大孔树脂吸附在黄酮及皂苷类成分分离纯化中的应用.时珍国医国药17 2.2006,17(2),第277页左栏第2部分. * |
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