CN1839773B - Method for making heterogeneous bone free of antibody - Google Patents
Method for making heterogeneous bone free of antibody Download PDFInfo
- Publication number
- CN1839773B CN1839773B CN2005100314052A CN200510031405A CN1839773B CN 1839773 B CN1839773 B CN 1839773B CN 2005100314052 A CN2005100314052 A CN 2005100314052A CN 200510031405 A CN200510031405 A CN 200510031405A CN 1839773 B CN1839773 B CN 1839773B
- Authority
- CN
- China
- Prior art keywords
- bone
- heterogeneous
- antigen
- graft
- water
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 210000000988 bone and bone Anatomy 0.000 title claims abstract description 73
- 238000000034 method Methods 0.000 title claims abstract description 18
- 201000010099 disease Diseases 0.000 claims abstract description 9
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims abstract description 9
- 241000700605 Viruses Species 0.000 claims abstract description 6
- 238000001514 detection method Methods 0.000 claims abstract description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 14
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 12
- 230000001054 cortical effect Effects 0.000 claims description 10
- 238000004140 cleaning Methods 0.000 claims description 6
- 238000007789 sealing Methods 0.000 claims description 4
- 241001465754 Metazoa Species 0.000 claims description 3
- 208000006735 Periostitis Diseases 0.000 claims description 3
- 210000003460 periosteum Anatomy 0.000 claims description 3
- 238000005238 degreasing Methods 0.000 claims 3
- 239000000243 solution Substances 0.000 claims 3
- 230000002045 lasting effect Effects 0.000 claims 2
- 101000937129 Drosophila melanogaster Cadherin-related tumor suppressor Proteins 0.000 claims 1
- 239000000427 antigen Substances 0.000 claims 1
- 102000036639 antigens Human genes 0.000 claims 1
- 108091007433 antigens Proteins 0.000 claims 1
- 210000002257 embryonic structure Anatomy 0.000 claims 1
- 239000000834 fixative Substances 0.000 claims 1
- 239000002504 physiological saline solution Substances 0.000 claims 1
- 238000002791 soaking Methods 0.000 claims 1
- 238000004506 ultrasonic cleaning Methods 0.000 claims 1
- 238000005406 washing Methods 0.000 claims 1
- 239000000463 material Substances 0.000 abstract description 20
- 238000002360 preparation method Methods 0.000 abstract description 11
- 238000001356 surgical procedure Methods 0.000 abstract description 6
- 238000004519 manufacturing process Methods 0.000 abstract description 3
- 230000001097 osteosynthetic effect Effects 0.000 abstract 2
- 230000000399 orthopedic effect Effects 0.000 abstract 1
- 241000283690 Bos taurus Species 0.000 description 5
- 230000001954 sterilising effect Effects 0.000 description 5
- 238000004659 sterilization and disinfection Methods 0.000 description 5
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 4
- 239000003814 drug Substances 0.000 description 4
- 239000007769 metal material Substances 0.000 description 4
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- 230000002159 abnormal effect Effects 0.000 description 3
- 230000001476 alcoholic effect Effects 0.000 description 3
- 230000002742 anti-folding effect Effects 0.000 description 3
- 238000006243 chemical reaction Methods 0.000 description 3
- 230000006835 compression Effects 0.000 description 3
- 238000007906 compression Methods 0.000 description 3
- 244000309465 heifer Species 0.000 description 3
- 229920003023 plastic Polymers 0.000 description 3
- 238000002604 ultrasonography Methods 0.000 description 3
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 2
- 206010002198 Anaphylactic reaction Diseases 0.000 description 2
- 208000005623 Carcinogenesis Diseases 0.000 description 2
- 229910000831 Steel Inorganic materials 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 230000001154 acute effect Effects 0.000 description 2
- 230000036783 anaphylactic response Effects 0.000 description 2
- 208000003455 anaphylaxis Diseases 0.000 description 2
- 230000002929 anti-fatigue Effects 0.000 description 2
- 230000036952 cancer formation Effects 0.000 description 2
- 231100000504 carcinogenesis Toxicity 0.000 description 2
- 239000013527 degreasing agent Substances 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 230000035876 healing Effects 0.000 description 2
- 239000007943 implant Substances 0.000 description 2
- 238000002513 implantation Methods 0.000 description 2
- 208000015181 infectious disease Diseases 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 210000003205 muscle Anatomy 0.000 description 2
- 238000003307 slaughter Methods 0.000 description 2
- 239000010959 steel Substances 0.000 description 2
- 210000001519 tissue Anatomy 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 206010011732 Cyst Diseases 0.000 description 1
- 206010018910 Haemolysis Diseases 0.000 description 1
- 241000521257 Hydrops Species 0.000 description 1
- 206010030113 Oedema Diseases 0.000 description 1
- 235000008331 Pinus X rigitaeda Nutrition 0.000 description 1
- 235000011613 Pinus brutia Nutrition 0.000 description 1
- 241000018646 Pinus brutia Species 0.000 description 1
- 229920001244 Poly(D,L-lactide) Polymers 0.000 description 1
- 206010067268 Post procedural infection Diseases 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- 208000031320 Teratogenesis Diseases 0.000 description 1
- 208000018756 Variant Creutzfeldt-Jakob disease Diseases 0.000 description 1
- 208000020538 atrophic muscular disease Diseases 0.000 description 1
- 208000005881 bovine spongiform encephalopathy Diseases 0.000 description 1
- 230000000711 cancerogenic effect Effects 0.000 description 1
- 231100000315 carcinogenic Toxicity 0.000 description 1
- 230000002490 cerebral effect Effects 0.000 description 1
- 210000000038 chest Anatomy 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 208000031513 cyst Diseases 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 230000003111 delayed effect Effects 0.000 description 1
- 238000006073 displacement reaction Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 230000008588 hemolysis Effects 0.000 description 1
- 210000000003 hoof Anatomy 0.000 description 1
- 238000007654 immersion Methods 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 230000007794 irritation Effects 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 210000005036 nerve Anatomy 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 238000004806 packaging method and process Methods 0.000 description 1
- 238000012856 packing Methods 0.000 description 1
- 230000007170 pathology Effects 0.000 description 1
- 229920001432 poly(L-lactide) Polymers 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 239000002510 pyrogen Substances 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000035943 smell Effects 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
Landscapes
- Materials For Medical Uses (AREA)
Abstract
本发明涉及一种制备用于制备骨外科处理中的移植填充材料和接骨固定物材料的方法。同时本发明还进一步涉及用这样的材料制备骨移植填充材料和接骨固定物的方法以及所制得的骨移植物和骨固定物。本发明的技术方案是在通常的检疫项目之外，还增加疯牛病病毒检测。本发明提供的一种去抗原异种骨的制备方法，确保生产的产品无疯牛病病毒及其他疫病，生产成本较现有方法低。骨移植或骨固定物的制备方法更完善，产品质量更有保障。The present invention relates to a method for the preparation of graft filling materials and osteosynthetic fixation materials for use in orthopedic surgical treatments. At the same time, the present invention further relates to methods for preparing bone graft filling materials and osteosynthetic fixtures using such materials, as well as the resulting bone grafts and bone fixtures. The technical scheme of the invention is to increase the detection of mad cow disease virus in addition to the usual quarantine items. The invention provides a method for preparing the antigen-removed heterogeneous bone, which ensures that the produced product is free from mad cow disease virus and other diseases, and the production cost is lower than that of the existing method. The preparation method of bone graft or bone fixation is more perfect, and the product quality is more guaranteed.
Description
Technical field
The present invention relates to a kind of preparation and be used for preparing the replanting filling material of Orthopeadic Surgery processing and the method for synthetism fixity material.The present invention simultaneously also further relates to method and prepared bone graft and the bone fixture with such material preparation bone replanting filling material and synthetism fixity.
Technical background
The material of using in the bone surgery mainly is two classes.
One class is a fixture materials.Except that being the nonmetallic materials seldom partly, most fixtures are metal material.Class of metal materials: as screw, round needle, intramedullary pin, blade plate, Cage etc., still in occupation of domestic and international greatly part market.Though they have higher mechanical strength and anti-fatigue performance, but satisfy the fixed needs of bone surgery, but complication such as they also may exist that implant is deteriorated, metal anaphylaxis and embedded material tumorigenesis; Generally all need second operation to take out; Especially on biomechanics, because the elastic modelling quantity gap of metal material and skeleton is too big, stress force shelter reaction is too strong, and on inside-fixture (steel plate) plane, cortical bone disuse atrophy and pine that the metal material below easily takes place become, and cause refracture; On the screw plane, easily fatigue fracture takes place or screw fractures; At the steel plate two ends, also easily fracture.Complication such as Cage has displacement, sinking, do not merge, infection and nerve root irritation take place.DIKFIX (Di Kang) screw that nonmetallic materials class fixture has BIOFIX (hundred is excellent) that Finland made by SR-PGA and SR-PLLA material and China to be made by super high molecular weight PDLLA material.They all are a kind of high molecular polymers, the reaction of local hydrops is often arranged and cause the aseptic cyst, can not secondary sterilization, cost an arm and a leg.The bone implant material of the same race that also has the Shanxi bone bank to produce in addition, but there is bone source difficulty in this material and may causes that human diseases propagates.Or utilize from the body bone, but this method has been damaged the healthy bone of patient from body.
Another kind of is replanting filling material, as the golden generation bone grafting spirit (RBX) of Kiel bone (moral), Oswestry bone (U.S.) and China.These materials all can only be used for " filling is damaged " and " promoting healing ".
Chinese patent ZL 98112370.8 discloses a kind of preparation method of xenogenesis bone fixture.It is to adopt qualified animal, gets its long bone in back 6 hours slaughtering, and divests periosteum, removes organic principles such as bone internal protein, fat, and preliminary working becomes rectangular, dull and stereotyped, the block blank of semicircle is inserted after the cleaning in 75% ethanol.Arrive this, made the material that is suitable for making xenogenesis bone fixture.Then, be machined into the fixture such as screw, pole of different size in the week, cleaning is placed on and seals in the container that fills with Precerving liquid, and with the Co of 15KGY
60Irradiation sterilization.
The xenogenesis bone fixture that foregoing invention provides our experiments show that it has enough mechanical strengths and anti-fatigue performance, and is good, nontoxic with the compatibility of tissue, non-carcinogenesis and anaphylaxis, no immunological rejection.
At present, because the appearance of bovine spongiform encephalopathy, countries in the world especially are the goods in source with the cattle to animal product, and quarantine control is very strict.
Summary of the invention
The present invention is the further improvement on the above-mentioned patent ZL98112370.8 of applicant basis.
Technical scheme of the present invention is outside common quarantine item, also increases crazy heifer disease virus and detects.
Need the regular hour because crazy heifer disease virus detects, therefore original technical scheme was got bone in interior 6 hours and is difficult to realize, through discovering, preserved under refrigerated condition, can prolong and get the bone time, and product quality is not all had influence.
In the former technical scheme, adopt 75% alcohol-pickled.Find after deliberation, adopt to be not less than 50% and can to realize goal of the invention to ethanol less than 75% concentration.And mechanical strength slightly improves, and the tension as 75% when alcohol-pickled, resistance to compression, anti-folding, antitorque ultimate load are respectively 139.9MPa, 178.42MPa, 22.2MPa, 117.77MPa; 50% tension, resistance to compression, anti-folding, antitorque ultimate load when alcohol-pickled then is 142.8MPa, 181.77MPa, 167.4MPa, 50MPa.Because the ethanol consumption that is used to soak is big, thereby adopt the ethanol of low concentration to reduce production costs significantly.
The present invention further provides the method for preparing bone transplanting or bone fixture with the heterogeneous bone free of antibody of above-mentioned preparation, concrete step is:
(1) get as stated above, in the four stars phase with interior preparation heterogeneous bone free of antibody, be processed into the fixture or the graft of required form;
(2) after water cleans, under aseptic condition, use the degreaser defat;
(3) reuse water cleans after the defat, and single preservation in the environment that normal saline or water are arranged; Use Co after the sealing
60Store for future use under the irradiation sterilization, room temperature.
Further improving is that processed and molded product first water in 2 hours cleans 2 times at least, and the product after the cleaning is changed clean container and entered aseptic by pass-through box, adds water with ultrasound wave then and cleans 2 times, continues 5 minutes at least at every turn.
The time of defat is not less than 24 hours in the 2nd step.
To in above-mentioned the 3rd step after defat, said cleaning is that ultrasound wave adds water and cleans 2 times and continue 5 minutes at least at every turn.
Degreaser can be general chloroform, acetone, chloroform, methanol etc.Co
60Irradiation sterilization can carry out according to national standard.
According to Chinese patent ZL98112370.8, the prepared fixture that just is used for bone surgery.The present invention then discloses with prepared heterogeneous bone free of antibody can also be used to prepare the bone surgery graft.The shape of graft can be graininess, also can be powder.
Heterogeneous bone free of antibody be can prepare with method provided by the invention and bone transplanting or bone fixture prepared,
The preparation method of a kind of heterogeneous bone free of antibody provided by the invention guarantees that the product of producing does not have crazy heifer disease virus and other eqpidemic diseases, and production cost is low than existing methods.The preparation method of bone transplanting or bone fixture is more perfect, and product quality is more secure.
Specific embodiment
Select to have more than 1 years old " the top grade cattle " of accurate government official's card and do not have " the first-class cattle " of extremity physiological defect to be the raw material object.Butchering mode (the clean body of shower, electric fiber crops cause dusk, assassinate blood-letting, clean thorax etc. is shedded, begun to speak in peeling) all carries out by the NY467-2001 standard.Get cerebral tissue behind the government official immediately and do pathology inspection and Western detection, cattle is temporarily put freezer, just gives after the feminine gender as a result and gets bone.When getting bones of limbs, peeling hair, meat, hoof and joint stay long bone of limbs to do.Cattle slaughter to the time of getting the bone immersion treatment should be less than 24 hours.Select fresh gloss, no abnormal mottle, deformity and defective, no fur muscle and dirt stick, the skeleton of no abnormal abnormal smells from the patient.Os Bovis seu Bubali packed into fill in the 50% alcoholic acid Plastic Drum.Fresh Os Bovis seu Bubali need be removed periosteum, muscle, spongy bone, only keeps cortical bone, handles and wants clean, thorough.The Os Bovis seu Bubali that pretreatment is good is processed into rectangular, dull and stereotyped, nahlock shape blank.After cleaning up with 50% alcoholic solution, classification is packed into and is filled in the 50% alcoholic acid different vessels.
Defat is carried out at aseptic.After the defat 24 hours, reuse ultrasound wave and water clean 2 times.
Product carries out single packing at aseptic, contains Precerving liquid in the plastic bag, adds 2 layers of plastic bag packaging, sealing after the sealing again.Use Co
60Store for future use under the room temperature behind the irradiation sterilization.
Resistance to compression ultimate load with the heterogeneous bone free of antibody of rectangular, dull and stereotyped, the nahlock shape blank of heterogeneous bone free of antibody preparation is 186.4～205.2MPa, anti-folding ultimate load 154.7～179.8MPa, tension ultimate load 117.1～139.1MPa, antitorque ultimate load 53.5～55MPa.Detection method is:
Heterogeneous bone free of antibody is successively in the attached sweet spring water of Shanghai Tongji University hospital, attached four hospitals of Dalian Medical Univ, Affiliated Hospital of Guangdong Pharmaceutical University, the Zhejiang Prov. Hospital of Traditional Chinese Medicine, Xiangye No. 2 Hospital of Central South University, an attached hospital of Hunan College of Traditional Chinese Medicine, People's Hospital, Hunan Prov., an attached hospital of University Of Nanhua, Guangdong Shi Jie hospital, Shaoshan City institute of traditional Chinese medicine, Yiyang City institute of traditional Chinese medicine, second the People's Hospital, Shaodong city, Changsha the 4th hospital, Changsha the 7th hospital, Yueyang Affiliated Hospital of Vocationl Technical College, First People's Hospital, Hengyang City, Chenzhou railway hospital, Zhangjiajie railway hospital etc. is distributed in more than 20 tame hospital clinicals of 5 provinces and regions and uses example (wherein being used to transplant 15 examples) surplus in the of about 400 altogether, through following up a case by regular visits to for many years, effect is satisfied: patient's whole body is all right, does not have because of product the bacterium person that causes the postoperative infection is arranged; The person of immediately pyrogen reaction thing take place after do not have implanting; Do not have the back of implantation acute general toxic reaction person is taken place; Do not have the back of implantation acute haemolysis person is taken place; Nothing is carcinogenic because of product, the teratogenesis person; Otch does not have infection; Press Yang Li people's standard, fracture is healing all, no delayed union, disunion and malunion.The 15 routine patients that are used to transplant observed 6～18 months, and bone merges, no prosthetic loosening, and no bone dissolving, no sequestrum forms.
Claims (6)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2005100314052A CN1839773B (en) | 2005-04-01 | 2005-04-01 | Method for making heterogeneous bone free of antibody |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2005100314052A CN1839773B (en) | 2005-04-01 | 2005-04-01 | Method for making heterogeneous bone free of antibody |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1839773A CN1839773A (en) | 2006-10-04 |
| CN1839773B true CN1839773B (en) | 2011-05-04 |
Family
ID=37029087
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2005100314052A Expired - Lifetime CN1839773B (en) | 2005-04-01 | 2005-04-01 | Method for making heterogeneous bone free of antibody |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1839773B (en) |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1188015A (en) * | 1996-12-09 | 1998-07-22 | 中国人民解放军第四军医大学第一附属医院 | Lump type defatted antigen-removing heterogenic bone grafting material and preparing method thereof |
| CN1228292A (en) * | 1998-03-09 | 1999-09-15 | 长沙铁路总公司长沙医院 | Heterogenic cortical endosteal fixator |
| CN1290182A (en) * | 1998-02-06 | 2001-04-04 | 骨科技术公司 | Osteoimplant and method for its manufacture |
| CN1463756A (en) * | 2002-06-06 | 2003-12-31 | 北京市创伤骨科研究所 | Method for preparation of artificial bone antigen for biological origin |
| CN1507925A (en) * | 2002-12-20 | 2004-06-30 | 上海组织工程研究与开发中心 | Use of decellularized, decalcitied bone as tissue engineered material |
-
2005
- 2005-04-01 CN CN2005100314052A patent/CN1839773B/en not_active Expired - Lifetime
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1188015A (en) * | 1996-12-09 | 1998-07-22 | 中国人民解放军第四军医大学第一附属医院 | Lump type defatted antigen-removing heterogenic bone grafting material and preparing method thereof |
| CN1290182A (en) * | 1998-02-06 | 2001-04-04 | 骨科技术公司 | Osteoimplant and method for its manufacture |
| CN1228292A (en) * | 1998-03-09 | 1999-09-15 | 长沙铁路总公司长沙医院 | Heterogenic cortical endosteal fixator |
| CN1463756A (en) * | 2002-06-06 | 2003-12-31 | 北京市创伤骨科研究所 | Method for preparation of artificial bone antigen for biological origin |
| CN1507925A (en) * | 2002-12-20 | 2004-06-30 | 上海组织工程研究与开发中心 | Use of decellularized, decalcitied bone as tissue engineered material |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1839773A (en) | 2006-10-04 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Vastel et al. | Effect of different sterilization processing methods on the mechanical properties of human cancellous bone allografts | |
| JP5053470B2 (en) | Graft prosthesis and materials and methods thereof | |
| CN101810855A (en) | II-type collagen joint cartilage fluid and preparation method thereof | |
| JP2002505144A (en) | Soft tissue xenograft | |
| CN105435307A (en) | Natural-tissue-derived decellularized and decalcified bone material and preparation method thereof | |
| CN106492285A (en) | Injectable Acellular cartilaginous matrix particulate and its application in implant | |
| CN107744413A (en) | The preparation method of new Valved allograft | |
| Breugem et al. | Is there evidence-based guidance for timing of soft tissue coverage of grade III B tibia fractures? | |
| RU2499612C2 (en) | Biological nasal bridge implant and method for making it | |
| JP5763790B2 (en) | Biological transplant material derived from mammalian cartilage tissue | |
| CN101492491A (en) | Method for preparing type II collagen mix powder | |
| CN1839773B (en) | Method for making heterogeneous bone free of antibody | |
| CN110101919A (en) | A kind of high-strength, fatigue-resistant low elastic modulus orthopaedics implant | |
| RU2722266C1 (en) | Lyophilized biological biodegradable mineralized osteoplastic material and method for production thereof | |
| CN201104913Y (en) | Homogeneous modified character bone hands surgery bone-fracture internal fixation machine | |
| CA2752245C (en) | Implants made of the inner core of drupes and/or palm tree fruits | |
| CN106075407B (en) | A kind of functional calcium preparation and preparation method thereof | |
| Mooradian | Allografts and xenografts in soft tissue repair: Current use and future trends | |
| RU2211685C2 (en) | Method for manufacturing carcassless biological prosthesis of cardiac aortic valve | |
| CN1073395C (en) | Heterogenic cortical endosteal fixator | |
| RU2332948C2 (en) | Method of revascularisation of large joints bone fragments | |
| SU1297833A1 (en) | Method of endoprosthesis of joints of hand fingers | |
| CN109452986A (en) | A kind of method that rat femur external fixator of variable rigidity promotes the healing of II phase of fracture | |
| Sh | Biological osteosynthesis as the treatment mode for multifragmental extra-articular fractures of lower limb long bones | |
| Vorobyov et al. | Autologous regenerative stimulants for bone allograft implantation |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| ASS | Succession or assignment of patent right |
Owner name: HUNAN HENGTIAN BIOTECHNOLOGY CO., LTD. Free format text: FORMER OWNER: ZHU TIANJING Effective date: 20120511 |
|
| C41 | Transfer of patent application or patent right or utility model | ||
| COR | Change of bibliographic data |
Free format text: CORRECT: ADDRESS; FROM: 410001 CHANGSHA, HUNAN PROVINCE TO: 412007 ZHUZHOU, HUNAN PROVINCE |
|
| TR01 | Transfer of patent right |
Effective date of registration: 20120511 Address after: 412007 A1-3 building, small and medium-sized enterprise promotion zone, Tianyuan District, Zhuzhou, Hunan Patentee after: Hunan Hengtian Biological Technology Co.,Ltd. Address before: 410001 Changsha seventh hospital, 39 Wuyi Road, Hunan, Changsha, China Patentee before: Zhu Tianjing |
|
| CX01 | Expiry of patent term |
Granted publication date: 20110504 |
|
| CX01 | Expiry of patent term |