CN1825121B - Plastic chip - Google Patents

Abstract

A plastic chip relates to the field of biochip technology and in vitro clinical diagnosis and detection technology, and can be applied to clinical multi-indicator and multi-person parallelized disease detection. It consists of the following components: upper fixture, lower fixture, chip strip, reaction plate. Among them, the chip strip is made of plastic with affinity to biologically active samples, and is detachable and freely combined. After the chip strip is fixed by the upper and lower clamps, a high-speed spotting instrument is used to construct a biological sample dot matrix on the chip strip, and then buckled into the reaction plate containing the serum or sample and the reaction solution to be tested for reaction. Compared with the prior art, the invention has the advantages of low background signal value, convenient manufacture and use, and low price.

Description

a plastic chip

technical field

The invention relates to the technical fields of biochip technology and in vitro clinical diagnosis and detection, more precisely, relates to a biochip suitable for multi-indicator and multi-person parallel detection.

Background technique

The concept of biochip originated from semiconductors and computer chips, mainly referring to DNA microarrays, oligonucleotide microarrays and protein microarrays coated on solid phase carriers. Currently common biochips are divided into three categories: gene chips, protein chips, and lab-on-a-chip. The main features of biochips are high-throughput, miniaturization and automation. They can analyze a large number of biomolecules in a short period of time, and quickly and accurately obtain biological information in samples. The detection efficiency is hundreds or thousands of times that of traditional detection methods. At present, there are two types of solid phase carriers commonly used for biochips at home and abroad: glass slides and nylon membranes. Glass slides are mainly used for gene chips, which have the advantages of low cost and low detection background. However, complex chemical treatments are required on the surface of slides before sample application, which is difficult to process and fragile. Although nylon membrane has good adsorption to biomolecules and helps to maintain the natural activity of biomolecules, it usually has a high background signal value. Currently, it mainly relies on imports and is expensive.

Therefore, there is an urgent need in this field to develop a biochip with a low background signal value, cheap and easy-to-use material as a solid phase carrier. Plastic is an ideal material for preparing biochips. The plastic has moderate protein adsorption capacity, which significantly overcomes the disadvantage of high background signal value of nylon membrane. Plastics are also easy to form into shapes, are not brittle, are inexpensive, and require minimal handling prior to sample placement. Therefore, we have developed a plastic chip using plastic as a material. In addition to the above-mentioned advantages of easy production and use, the chip strip can also be disassembled and combined freely, so that the indicators of clinical testing and the number of patients can be combined freely, which is more suitable for clinical practice. actual need for testing.

Contents of the invention

The object of the present invention is to solve the above problems and provide a multi-indicator, multi-person parallelized disease detection biochip that can be applied in clinic. A chip system with low background, low cost and easy operation is provided.

The invention provides a plastic biochip, which is characterized in that it contains the following components:

- a chip strip (1), the chip strip has a raised horizontal end surface and a base for clamping and fixing the upper and lower clamps;

- the upper fixture (2), the upper fixture is a sealed box with a framed bottom on four sides, and at the same time, the four sides have protrusions or holes for engaging with the lower fixture;

- the lower clamp (3), the lower clamp has a hole corresponding to the horizontal end face of the chip protrusion, and at the same time, the four sides have protrusions or hole grooves for easy engagement with the upper clamp;

- Reaction plate (4), the reaction plate has a sealing hole corresponding to the protruding end surface of the chip strip, and a reaction solution can be added in the hole to facilitate the contact of the end surface of the chip strip for biochemical reaction;

Wherein, each component is separated from each other, and when assembled together, the various parts of the chip strip (1), the upper and lower clamps (2, 3) and the reaction plate (4) correspond to each other and are tightly combined.

In a preferred example, the shapes, sizes, and positions of the hole arrays of the chip strip (1), the upper clamp (2), the lower clamp (3), and the reaction plate (4) are in one-to-one correspondence and can be completely matched.

In another preferred embodiment, the chip strip (1) is made of polystyrene material or other materials that can adsorb biologically active samples (DNA, protein, etc.).

In another preferred example, the chip strip (1) can be easily disassembled from the upper and lower fixtures (2, 3), and the required detection portions can be freely combined.

In another preferred example, the number of holes corresponding to the lower fixture (3) and the reaction plate (4) is 48, 96, 128, 384.

In another preferred example, the shape and size of the chip strip (1) are consistent with the size and shape of the hole of the lower clamp (3), and the shape is basically rectangular, hexagonal, octagonal or circular.

In another preferred example, after the assembly of the chip strip (1) and the upper and lower clamps (2, 3) is completed, it can be reversed in the reaction plate (4) to perform various reactions such as immunity, hybridization, and biochemistry.

In another preferred example, the materials of the upper and lower clamps (2, 3) and the reaction plate (4) are selected from the following group: plastic, rubber or metal and all available materials.

Description of drawings

The accompanying drawing is an exploded view of an example of the present invention.

specific implementation plan

As shown in the figure, the biochip in the present invention includes four components of upper clamp 2, lower clamp 3, chip strip 1, and reaction plate 4, each component is independent of each other, in addition, also contains optional fixing means (protrusion or hole Grooves, etc.) for fixing the above components together.

Below in conjunction with specific embodiment, further illustrate the present invention.

Example

(1) Put the chip strip 1 into the corresponding hole of the lower fixture, tighten the upper and lower fixtures 2 and 3, and fix the chip strip 1.

(2) Construct a dot matrix of biological samples (DNA, protein, etc.) on the upper end surface of the chip strip 1 with a high-speed spotting instrument.

(3) Add the serum samples to be tested and related reaction solutions to the 4 wells of the reaction plate in sequence, and buckle the tightly closed upper and lower clamps and the chip strip assembly upside down into the reaction plate for incubation and reaction.

(4) Rinse according to the conventional washing method.

(5) Put the washed upper and lower fixtures 2, 3 and chip strip 1 assembly into the detector for detection.

It should be understood that after reading the above teaching content of the present invention, those skilled in the art can make various changes or modifications to the present invention, and these equivalent forms also fall within the scope defined by the appended claims of the present application.

Claims (8)

1. A plastic biochip, characterized in that it contains the following components: A chip strip (1), the chip strip has a raised horizontal end face and a base for clamping and fixing by the upper clamp (2) and the lower clamp (3); An upper fixture (2), the upper fixture is a sealed box with a framed bottom on four sides, and the four sides are provided with protrusions or slots to facilitate engagement with the lower fixture; a lower clamp (3), the lower clamp has a hole corresponding to the raised horizontal end surface of the chip strip, and at the same time, the four sides have protrusions or hole grooves to facilitate engagement with the upper clamp; A reaction plate (4), the reaction plate has a sealed hole corresponding to the raised horizontal end face of the chip strip, adding a reaction solution in the hole to facilitate the contact of the raised horizontal end face of the chip strip for immunoreaction or hybridization reaction; Wherein, each component is separated from each other, and when assembled together, the various parts of the chip strip (1), the upper clamp (2), the lower clamp (3) and the reaction plate (4) correspond to each other and are tightly combined; After the chip strip (1) is assembled with the upper fixture (2) and the lower fixture (3), it is turned upside down in the reaction plate (4) to carry out immunoreaction or hybridization reaction. 2. biochip according to claim 1, is characterized in that, the position of chip strip (1), upper clamp (2), lower clamp (3), reaction plate (4) shape, size, hole array are all One-to-one correspondence, can completely match. 3. The biochip according to claim 1, characterized in that, the material of the chip strip (1) is selected from materials with adsorption properties for DNA and protein biologically active samples. 4. The biochip according to claim 3, characterized in that, the material of the chip strip (1) is polystyrene material. 5. The biochip according to claim 1, characterized in that the chip strip (1) is disassembled from the upper clamp (2) and the lower clamp (3), and the required detection portions are freely combined. 6. The biochip according to claim 1, characterized in that the number of holes corresponding to the lower fixture (3) and the reaction plate (4) is 48, 96, 128, 384. 7. The biochip according to claim 1, characterized in that, the shape and size of the chip strip (1) are consistent with the shape and size of the hole of the lower fixture (3), and the external shape is substantially rectangular, hexagonal, Octagon or circle. 8. The biochip according to claim 1, characterized in that the materials of the upper clamp (2), the lower clamp (3) and the reaction plate (4) are selected from the group consisting of plastic, rubber or metal.

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