CN1818651B - Rabbit Anti-Goose IgY+IgY(△Fc)(H+L) Horseradish Peroxidase Labeled Antibody - Google Patents
Rabbit Anti-Goose IgY+IgY(△Fc)(H+L) Horseradish Peroxidase Labeled Antibody Download PDFInfo
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- CN1818651B CN1818651B CN 200610009804 CN200610009804A CN1818651B CN 1818651 B CN1818651 B CN 1818651B CN 200610009804 CN200610009804 CN 200610009804 CN 200610009804 A CN200610009804 A CN 200610009804A CN 1818651 B CN1818651 B CN 1818651B
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- 241000283973 Oryctolagus cuniculus Species 0.000 title claims abstract description 23
- 108010001336 Horseradish Peroxidase Proteins 0.000 title claims abstract description 20
- 102000004190 Enzymes Human genes 0.000 claims abstract description 30
- 108090000790 Enzymes Proteins 0.000 claims abstract description 30
- 241000272814 Anser sp. Species 0.000 claims abstract description 20
- 238000002965 ELISA Methods 0.000 claims description 7
- 230000000694 effects Effects 0.000 claims description 3
- 239000000758 substrate Substances 0.000 claims description 2
- 238000001514 detection method Methods 0.000 abstract description 10
- 238000000034 method Methods 0.000 abstract description 10
- 210000002969 egg yolk Anatomy 0.000 abstract description 8
- 238000002360 preparation method Methods 0.000 abstract description 6
- JQWHASGSAFIOCM-UHFFFAOYSA-M sodium periodate Chemical compound [Na+].[O-]I(=O)(=O)=O JQWHASGSAFIOCM-UHFFFAOYSA-M 0.000 abstract description 6
- 201000010099 disease Diseases 0.000 abstract description 4
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract description 4
- 108060003951 Immunoglobulin Proteins 0.000 abstract description 3
- 230000003053 immunization Effects 0.000 abstract description 3
- 102000018358 immunoglobulin Human genes 0.000 abstract description 3
- 208000035473 Communicable disease Diseases 0.000 abstract description 2
- 238000002372 labelling Methods 0.000 abstract description 2
- 238000005185 salting out Methods 0.000 abstract 2
- 238000004587 chromatography analysis Methods 0.000 abstract 1
- 238000001742 protein purification Methods 0.000 abstract 1
- 108010001160 IgY Proteins 0.000 description 32
- 238000001556 precipitation Methods 0.000 description 8
- 239000003480 eluent Substances 0.000 description 6
- 239000000047 product Substances 0.000 description 5
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 4
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 description 4
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical class N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 4
- 238000010612 desalination reaction Methods 0.000 description 4
- 238000003018 immunoassay Methods 0.000 description 4
- 238000002347 injection Methods 0.000 description 4
- 239000007924 injection Substances 0.000 description 4
- 210000002966 serum Anatomy 0.000 description 4
- 239000006228 supernatant Substances 0.000 description 4
- 239000001913 cellulose Substances 0.000 description 3
- 230000002255 enzymatic effect Effects 0.000 description 3
- JRBJSXQPQWSCCF-UHFFFAOYSA-N 3,3'-Dimethoxybenzidine Chemical compound C1=C(N)C(OC)=CC(C=2C=C(OC)C(N)=CC=2)=C1 JRBJSXQPQWSCCF-UHFFFAOYSA-N 0.000 description 2
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- 239000002671 adjuvant Substances 0.000 description 2
- 239000000427 antigen Substances 0.000 description 2
- 102000036639 antigens Human genes 0.000 description 2
- 238000009360 aquaculture Methods 0.000 description 2
- 244000144974 aquaculture Species 0.000 description 2
- 238000010241 blood sampling Methods 0.000 description 2
- 239000000872 buffer Substances 0.000 description 2
- 229920002678 cellulose Polymers 0.000 description 2
- 230000001186 cumulative effect Effects 0.000 description 2
- 239000008367 deionised water Substances 0.000 description 2
- 229910021641 deionized water Inorganic materials 0.000 description 2
- 238000000502 dialysis Methods 0.000 description 2
- 238000002649 immunization Methods 0.000 description 2
- 238000011532 immunohistochemical staining Methods 0.000 description 2
- 238000005342 ion exchange Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 239000008363 phosphate buffer Substances 0.000 description 2
- 239000011734 sodium Substances 0.000 description 2
- 239000007974 sodium acetate buffer Substances 0.000 description 2
- 229910000029 sodium carbonate Inorganic materials 0.000 description 2
- 238000007920 subcutaneous administration Methods 0.000 description 2
- 210000003462 vein Anatomy 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 240000006927 Foeniculum vulgare Species 0.000 description 1
- 235000004204 Foeniculum vulgare Nutrition 0.000 description 1
- 229920001503 Glucan Polymers 0.000 description 1
- 108010093096 Immobilized Enzymes Proteins 0.000 description 1
- 229910021529 ammonia Inorganic materials 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 150000001450 anions Chemical class 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 230000008076 immune mechanism Effects 0.000 description 1
- 238000001114 immunoprecipitation Methods 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 230000002045 lasting effect Effects 0.000 description 1
- TWNIBLMWSKIRAT-VFUOTHLCSA-N levoglucosan Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@H]2CO[C@@H]1O2 TWNIBLMWSKIRAT-VFUOTHLCSA-N 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
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- Peptides Or Proteins (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
本发明提供的是兔抗鹅IgY+IgY(ΔFc)(H+L)辣根过氧化物酶标记抗体及其制备方法。本发明辣根过氧化物酶标记兔抗鹅IgY+IgY(ΔFc)(H+L)抗体是利用盐析、层析等蛋白纯化技术和酶标记技术，在提纯鹅卵黄免疫球蛋白的基础上，免疫家兔制备并纯化兔抗鹅IgY+IgY(ΔFc)(H+L)抗体，再通过改良过碘酸钠法将其与辣根过氧化物酶结合，并采用盐析及Sephodex G200纯化，从而制备辣根过氧化物酶标记兔抗鹅IgY+IgY(ΔFc)(H+L)抗体。应用此酶标记抗体可以建立多种鹅类疫病检测技术，预防鹅类多种传染病。The invention provides a rabbit anti-goose IgY+IgY(ΔFc)(H+L) horseradish peroxidase-labeled antibody and a preparation method thereof. The horseradish peroxidase-labeled rabbit anti-goose IgY+IgY(ΔFc)(H+L) antibody of the present invention uses protein purification techniques such as salting out and chromatography and enzyme labeling techniques, on the basis of purifying goose yolk immunoglobulin , Prepare and purify rabbit anti-goose IgY+IgY(ΔFc)(H+L) antibody by immunizing rabbits, then combine it with horseradish peroxidase through improved sodium periodate method, and purify by salting out and Sephodex G200 , so as to prepare horseradish peroxidase-labeled rabbit anti-goose IgY+IgY(ΔFc)(H+L) antibody. The enzyme-labeled antibody can be used to establish a variety of goose epidemic disease detection techniques and prevent various goose infectious diseases.
Description
(1) technical field
What the present invention relates to is a kind of enzyme antibody bond and preparation method thereof, specifically (H+L) antibody of the anti-goose IgY+IgY of horseradish peroxidase-labeled rabbit (Δ Fc).
(2) background technology
The enzyme immunoassay technology be with the reaction amplification of enzyme and Ag-Ab specificity binding characteristic be principle, based on a kind of novel detection technique of enzyme antibody and/or antigen bond.It comprises enzyme immunohistochemical staining and enzyme immunoassay technology (comprising solid and non-immobilized enzyme immunoassay technology), and the former is mainly used in the immune detection of tissue, cell, and the latter is mainly used in the detection of antigen/antibody.
Enzyme linked immunosorbent assay (ELISA) is with fastest developing speed in the enzyme immunoassay technology, most widely used a kind of, and it becomes a kind of generally acknowledged detection technique gradually with quick, accurate, sensitive characteristics.At present, this detection technique is used in the part animal epidemic detects, but only has the detection of only a few goose class disease to use this technology.Reason is a lot, is the one of the main reasons that hinders its development but wherein lack the enzymic-labelled antibody that a kind of high-quality commercialization produces.
China is the foster fowl big country in the world, and the main goose class in the world is cultured ground and country of consumption especially, and lasting, stable, the sound development of goose class aquaculture not only are directly connected to China's rural development, increasing peasant income, and are related to the sustainable and stable development of China's animal husbandry.And the anti-goose antibody of enzyme labeling is not only built the material platform for the detection of goose class eqpidemic disease, and more the research of the whole immune mechanism of goose class provides the material support, and finally provides technology platform for the development of goose class aquaculture.
(3) summary of the invention
The object of the present invention is to provide (H+L) antibody of the anti-goose IgY+IgY of horseradish peroxidase-labeled rabbit (Δ Fc).
The object of the present invention is achieved like this: employed abbreviation is represented respectively among the present invention: PBS: phosphate buffer; PB: phosphate buffer; DE52: cellulose anion exchanger; Sephodex G200: glucosan G200; IgY: immunoglobulin Y: IgY (Δ Fc): immunoglobulin Y (Δ Fc).
The anti-goose IgY+IgY of rabbit of the present invention (Δ Fc) (H+L) horseradish peroxidase-labeled antibody is:
(1) it is by (H+L) the enzyme antibody bond formed of antibody of horseradish peroxidase and the anti-goose IgY+IgY of rabbit (Δ Fc), and it can combine with goose immune globulin IgY and IgY (Δ Fc) specificity, and can make the substrate colour developing of horseradish peroxidase; (2) it be mole ratio between 1.0-2.0, enzymatic activity＞1000U/mg (face connection fennel ammonia process), ELISA working concentration＞1: 1000, the product of Weston-Blotting working concentration＞1: 500.
The anti-goose IgY+IgY of rabbit of the present invention (Δ Fc) (H+L) horseradish peroxidase-labeled antibody is to adopt such method to prepare:
1) purifying goose Yolk immunoglobulin IgY and IgY (Δ Fc)
In v/v is yolk: PBS (pH7.40.01mol/L): the ratio mixing of chloroform=1: 2: 3, the centrifugal 30min of 3000r/min, get supernatant, be respectively 18%, 14%, 9%, 14% anhydrous Na 2SO4 saltouts successively with final concentration (w/v), the centrifugal 30min of 3000r/min, wherein, get supernatant for the third time, the inferior precipitation of getting of its excess-three, the last precipitation suspends with an amount of PBS, 4 ℃ of PBS dialysis 72h, with the centrifugal 10～15min of dialysate 2000r/min, (PH7.4 0.01mol/L) is eluent, crosses DE with PB
52Cellulose column, 1ml/min, 1 pipe/5min collects first peak;
2) (H+L) preparation and the purifying of antibody of the anti-goose IgY+IgY of rabbit (Δ Fc)
Head exempts from, and Freund's complete adjuvant and goose Yolk immunoglobulin (0.4mg/ml) are that 1: 1 ratio is mixed 2ml/ the subcutaneous multi-point injection of rabbit back in v/v; Every 14～28d booster immunization, two exempt to use instead incomplete Freund later on, and the dosage mode is the same; Three exempt from 1ml (0.4mg/ml) auricular vein injection, the heart blood sampling, and 37 ℃, 1h, 4 ℃ are spent the night and separate out and collect serum; 10ml serum is saltoutd 3 times successively through 50% and 33% saturated ammonium sulfate, and ((0.01mol/L pH7.4) crosses DE for eluent with PB for 0.01mol/L, pH7.4) dissolving and to its desalination of dialysing with the PBS of 2～4ml for last precipitation
52The ion exchange column purifying, 1ml/min, 1 pipe/5min collects first peak;
3) improvement sodium periodate legal system is equipped with (H+L) antibody of the anti-goose IgY+IgY of horseradish peroxidase-labeled rabbit (Δ Fc)
N * 4mg horseradish peroxidase is dissolved in N * 1ml deionized water, adds 200-400 μ lNaIO
4(0.1mol/L), 20～25 ℃ are stirred 20min, in sodium-acetate buffer (pH4.4,0.001mol/L) 4 ℃ of dialysed overnight, the anti-goose antibody of rabbit that adds N * mg purifying, sodium carbonate buffer (pH9.5) the adjusting pH value that adds 0.2mol/L immediately causes 9-9.5, and 20～25 ℃ are stirred 2h (annotate: N is a constant);
4) purifying of label
Above-mentioned label is equally divided into 2 parts, a with the 50% saturated ammonium sulfate 30min that saltouts, the centrifuging and taking precipitation be partially soluble in a small amount of PBS (0.01mol/L, pH7.4), and in the PBS desalination of dialysing; Another part with PB (0.02mol/L, pH7.2) dialysed overnight, and be that eluent is crossed the SephodexG200 chromatographic column with it, 1ml/15min, the 2ml/ pipe merges two parts;
5) Quality Identification of enzymic-labelled antibody
280nm and 403nm measure its OD value, calculate its IgG content ((A280-A403 * 0.3) * 0.62mg/ml), enzyme content (A403 * 0.4mg/ml), mole ratio ((enzyme content/IgG content) * 4), bond mark rate (A403/A280 * 100%) enzyme conjugates productive rate (enzyme content * bond cumulative volume/add at first enzyme amount), the dianisidine method is measured the enzyme activity that label reaches, and directly ELISA surveys legal its best working concentration.When the enzyme conjugates productive rate of enzymic-labelled antibody between the 30%-60%, mole ratio between 1.0-2.0, enzyme conjugates mark rate＞60%, enzymatic activity＞1000U/mg, working concentration＞1: 1000 o'clock meets preparation standard.
Adopt method of the present invention can be used for:
1. this method can be used for purifying goose class Yolk immunoglobulin IgY and IgY (Δ Fc);
2. this method can be used for the Quality Identification of the anti-goose horseradish peroxidase-labeled of rabbit hole body;
The product that adopts the present invention to produce can be used for:
1. this product can be used for goose class relevant enzyme immunohistochemical staining.
2. this product can be used for goose class relevant various even phase and the non-even detection technique of immuno-enzymatic mutually, mainly comprises various ELISA tests, Weston-Blotting, the test of enzyme immunoprecipitation etc.
Product advantage of the present invention shows: use this enzymic-labelled antibody and can set up multiple goose class eqpidemic disease detection technique, the multiple infectious disease of prevention goose class.
(4) embodiment
1) purifying goose Yolk immunoglobulin IgY and IgY (Δ Fc)
In v/v is yolk: PBS (pH7.40.01mol/L): the ratio mixing of chloroform=1: 2: 3, the centrifugal 30min of 3000r/min, get supernatant, (w/v) is respectively 18% with final concentration, 14%, 9%, 14% anhydrous Na 2SO4 saltouts successively, the centrifugal 30min of 3000r/min wherein, gets supernatant for the third time, the inferior precipitation of getting of its excess-three, the last precipitation suspends with an amount of PBS, and 4 ℃ of PBS dialysis 72h are with the centrifugal 10～15min of dialysate 2000r/min, with PB (PH7.4,0.01mol/L) be eluent, cross DE 52 cellulose columns, 1ml/min, 1 pipe/5min collects first peak;
2) (H+L) preparation and the purifying of antibody of the anti-goose IgY+IgY of rabbit (Δ Fc)
Head exempts from, and Freund's complete adjuvant and goose Yolk immunoglobulin (0.4mg/ml) are that 1: 1 ratio is mixed 2ml/ the subcutaneous multi-point injection of rabbit back in v/v; Every 14～28d booster immunization, two exempt to use instead incomplete Freund later on, and the dosage mode is the same; Three exempt from 1ml (0.4mg/ml) auricular vein injection, the heart blood sampling, and 37 ℃, 1h, 4 ℃ are spent the night and separate out and collect serum.10ml serum is saltoutd 3 times successively through 50% and 33% saturated ammonium sulfate, and ((0.01mol/L pH7.4) crosses DE for eluent with PB for 0.01mol/L, pH7.4) dissolving and to its desalination of dialysing with the PBS of 2～4ml for last precipitation
52The ion exchange column purifying, 1ml/min, 1 pipe/5min collects first peak;
3) improvement sodium periodate legal system is equipped with (H+L) antibody of the anti-goose IgY+IgY of horseradish peroxidase-labeled rabbit (Δ Fc)
N * 4mg horseradish peroxidase is dissolved in N * 1ml deionized water, adds 200-400 μ lNaIO
4(0.1mol/L), 20～25 ℃ are stirred 20min, in sodium-acetate buffer (pH4.4,0.001mol/L) 4 ℃ of dialysed overnight, the anti-goose antibody of rabbit that adds N * mg purifying, sodium carbonate buffer (pH9.5) the adjusting pH value that adds 0.2mol/L immediately causes 9-9.5, and 20～25 ℃ are stirred 2h (annotate: N is a constant);
4) purifying of label
Above-mentioned label is equally divided into 2 parts, a with the 50% saturated ammonium sulfate 30min that saltouts, the centrifuging and taking precipitation be partially soluble in a small amount of PBS (0.01mol/L, pH7.4), and in the PBS desalination of dialysing; Another part with PB (0.02mol/L, pH7.2) dialysed overnight, and be that eluent is crossed the SephodexG200 chromatographic column with it, 1ml/15min, the 2ml/ pipe merges two parts;
5) Quality Identification of enzymic-labelled antibody
280nm and 403nm measure its OD value, calculate its IgG content ((A280-A403 * 0.3) * 0.62mg/ml), enzyme content (A403 * 0.4mg/ml), mole ratio ((enzyme content/IgG content) * 4), bond mark rate (A403/A280 * 100%) enzyme conjugates productive rate (enzyme content * bond cumulative volume/add at first enzyme amount), the dianisidine method is measured the enzyme activity that label reaches, and directly ELISA surveys legal its best working concentration.When the enzyme conjugates productive rate of enzymic-labelled antibody between the 30%-60%, mole ratio between 1.0-2.0, enzyme conjugates mark rate＞60%, enzymatic activity＞1000U/mg, working concentration＞1: 1000 o'clock meets preparation standard.
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| CN 200610009804 CN1818651B (en) | 2006-03-13 | 2006-03-13 | Rabbit Anti-Goose IgY+IgY(△Fc)(H+L) Horseradish Peroxidase Labeled Antibody |
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| CN 200610009804 CN1818651B (en) | 2006-03-13 | 2006-03-13 | Rabbit Anti-Goose IgY+IgY(△Fc)(H+L) Horseradish Peroxidase Labeled Antibody |
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Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101762689A (en) * | 2008-12-24 | 2010-06-30 | 中国农业大学 | Method for measuring content of IgY in yolk and/or serum |
| CN101899110B (en) * | 2010-07-16 | 2012-08-08 | 浙江大学 | Method for separating immune globulin IgY(delta Fc) from goose blood |
| CN117384295B (en) * | 2023-12-13 | 2024-03-08 | 北京索莱宝科技有限公司 | Mouse anti-goose IgY monoclonal antibody and application thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2001088162A2 (en) * | 2000-05-16 | 2001-11-22 | Genway Biotech, Inc. | Methods and vectors for generating antibodies in avian species and uses therefor |
| CN1438245A (en) * | 2003-02-01 | 2003-08-27 | 郭占军 | Infectious causative-agent related egg-yolk antibody preparation and use thereof |
| CN1509188A (en) * | 2001-05-15 | 2004-06-30 | �����������ֶ� | Immunoconjugates made of egg yolk antibodies, methods for their preparation and their use in diagnosis and therapy |
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2006
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2001088162A2 (en) * | 2000-05-16 | 2001-11-22 | Genway Biotech, Inc. | Methods and vectors for generating antibodies in avian species and uses therefor |
| CN1509188A (en) * | 2001-05-15 | 2004-06-30 | �����������ֶ� | Immunoconjugates made of egg yolk antibodies, methods for their preparation and their use in diagnosis and therapy |
| CN1438245A (en) * | 2003-02-01 | 2003-08-27 | 郭占军 | Infectious causative-agent related egg-yolk antibody preparation and use thereof |
Non-Patent Citations (2)
| Title |
|---|
| 贺云霞，等.鹅卵黄IgG的纯化及兔抗鹅IgG酶标抗体的制备.中国预防兽医学报27 5.2005,27(5),412-414. |
| 贺云霞,等.鹅卵黄IgG的纯化及兔抗鹅IgG酶标抗体的制备.中国预防兽医学报27 5.2005,27(5),412-414. * |
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Inventor after: Wang Junwei Inventor after: Sun Lingshuang Inventor after: Yu Tianfei Inventor before: Wang Junwei Inventor before: Sun Lingshuang |
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