CN1868484A - Method and device for high efficiency separation of puerarin - Google Patents
Method and device for high efficiency separation of puerarin Download PDFInfo
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- CN1868484A CN1868484A CNA200610088090XA CN200610088090A CN1868484A CN 1868484 A CN1868484 A CN 1868484A CN A200610088090X A CNA200610088090X A CN A200610088090XA CN 200610088090 A CN200610088090 A CN 200610088090A CN 1868484 A CN1868484 A CN 1868484A
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- RXUWDKBZZLIASQ-UHFFFAOYSA-N Puerarin Natural products OCC1OC(Oc2c(O)cc(O)c3C(=O)C(=COc23)c4ccc(O)cc4)C(O)C(O)C1O RXUWDKBZZLIASQ-UHFFFAOYSA-N 0.000 title claims abstract description 61
- HKEAFJYKMMKDOR-VPRICQMDSA-N puerarin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1C1=C(O)C=CC(C2=O)=C1OC=C2C1=CC=C(O)C=C1 HKEAFJYKMMKDOR-VPRICQMDSA-N 0.000 title claims abstract description 61
- 238000000034 method Methods 0.000 title claims abstract description 30
- 238000000926 separation method Methods 0.000 title claims abstract description 26
- 238000002425 crystallisation Methods 0.000 claims abstract description 70
- 230000008025 crystallization Effects 0.000 claims abstract description 70
- 239000002904 solvent Substances 0.000 claims abstract description 11
- 239000012530 fluid Substances 0.000 claims abstract description 10
- 238000000605 extraction Methods 0.000 claims abstract description 8
- IDGUHHHQCWSQLU-UHFFFAOYSA-N ethanol;hydrate Chemical compound O.CCO IDGUHHHQCWSQLU-UHFFFAOYSA-N 0.000 claims abstract description 4
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 31
- 238000007789 sealing Methods 0.000 claims description 3
- 239000012046 mixed solvent Substances 0.000 abstract description 7
- 230000008569 process Effects 0.000 abstract description 6
- 239000000284 extract Substances 0.000 abstract description 4
- 238000004519 manufacturing process Methods 0.000 abstract description 4
- 230000008901 benefit Effects 0.000 abstract description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 14
- 239000000047 product Substances 0.000 description 13
- 238000005516 engineering process Methods 0.000 description 9
- 239000013078 crystal Substances 0.000 description 7
- 238000004128 high performance liquid chromatography Methods 0.000 description 7
- 239000012043 crude product Substances 0.000 description 6
- 239000007787 solid Substances 0.000 description 6
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 5
- 238000000194 supercritical-fluid extraction Methods 0.000 description 5
- 238000000746 purification Methods 0.000 description 4
- PNEYBMLMFCGWSK-UHFFFAOYSA-N Alumina Chemical compound [O-2].[O-2].[O-2].[Al+3].[Al+3] PNEYBMLMFCGWSK-UHFFFAOYSA-N 0.000 description 3
- 239000000287 crude extract Substances 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- BOJKULTULYSRAS-OTESTREVSA-N Andrographolide Chemical compound C([C@H]1[C@]2(C)CC[C@@H](O)[C@]([C@H]2CCC1=C)(CO)C)\C=C1/[C@H](O)COC1=O BOJKULTULYSRAS-OTESTREVSA-N 0.000 description 2
- 229960000583 acetic acid Drugs 0.000 description 2
- ASLUCFFROXVMFL-UHFFFAOYSA-N andrographolide Natural products CC1(CO)C(O)CCC2(C)C(CC=C3/C(O)OCC3=O)C(=C)CCC12 ASLUCFFROXVMFL-UHFFFAOYSA-N 0.000 description 2
- MOLPUWBMSBJXER-YDGSQGCISA-N bilobalide Chemical compound O([C@H]1OC2=O)C(=O)[C@H](O)[C@@]11[C@@](C(C)(C)C)(O)C[C@H]3[C@@]21CC(=O)O3 MOLPUWBMSBJXER-YDGSQGCISA-N 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 229930003944 flavone Natural products 0.000 description 2
- 235000011949 flavones Nutrition 0.000 description 2
- 229930182486 flavonoid glycoside Natural products 0.000 description 2
- 150000007955 flavonoid glycosides Chemical class 0.000 description 2
- 238000002955 isolation Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 239000003960 organic solvent Substances 0.000 description 2
- 238000005192 partition Methods 0.000 description 2
- 230000001376 precipitating effect Effects 0.000 description 2
- 239000001397 quillaja saponaria molina bark Substances 0.000 description 2
- 238000001953 recrystallisation Methods 0.000 description 2
- 239000011347 resin Substances 0.000 description 2
- 229920005989 resin Polymers 0.000 description 2
- 229930182490 saponin Natural products 0.000 description 2
- 150000007949 saponins Chemical class 0.000 description 2
- 238000001179 sorption measurement Methods 0.000 description 2
- 229910001220 stainless steel Inorganic materials 0.000 description 2
- 239000010935 stainless steel Substances 0.000 description 2
- -1 terpene lactones Chemical class 0.000 description 2
- 235000007586 terpenes Nutrition 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 1
- 244000046146 Pueraria lobata Species 0.000 description 1
- 235000010575 Pueraria lobata Nutrition 0.000 description 1
- 229910000831 Steel Inorganic materials 0.000 description 1
- GAMYVSCDDLXAQW-AOIWZFSPSA-N Thermopsosid Natural products O(C)c1c(O)ccc(C=2Oc3c(c(O)cc(O[C@H]4[C@H](O)[C@@H](O)[C@H](O)[C@H](CO)O4)c3)C(=O)C=2)c1 GAMYVSCDDLXAQW-AOIWZFSPSA-N 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 230000001476 alcoholic effect Effects 0.000 description 1
- PYKYMHQGRFAEBM-UHFFFAOYSA-N anthraquinone Chemical class CCC(=O)c1c(O)c2C(=O)C3C(C=CC=C3O)C(=O)c2cc1CC(=O)OC PYKYMHQGRFAEBM-UHFFFAOYSA-N 0.000 description 1
- 150000004056 anthraquinones Chemical class 0.000 description 1
- 230000001093 anti-cancer Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000001311 chemical methods and process Methods 0.000 description 1
- 238000004440 column chromatography Methods 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000000857 drug effect Effects 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 239000000469 ethanolic extract Substances 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 150000002212 flavone derivatives Chemical class 0.000 description 1
- 150000002213 flavones Chemical class 0.000 description 1
- 239000012362 glacial acetic acid Substances 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 241000411851 herbal medicine Species 0.000 description 1
- 230000008676 import Effects 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 238000000622 liquid--liquid extraction Methods 0.000 description 1
- 230000010534 mechanism of action Effects 0.000 description 1
- 125000004108 n-butyl group Chemical group [H]C([H])([H])C([H])([H])C([H])([H])C([H])([H])* 0.000 description 1
- 229950000845 politef Drugs 0.000 description 1
- 238000002953 preparative HPLC Methods 0.000 description 1
- 238000010926 purge Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000000638 solvent extraction Methods 0.000 description 1
- 239000010959 steel Substances 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 230000009897 systematic effect Effects 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- VHBFFQKBGNRLFZ-UHFFFAOYSA-N vitamin p Natural products O1C2=CC=CC=C2C(=O)C=C1C1=CC=CC=C1 VHBFFQKBGNRLFZ-UHFFFAOYSA-N 0.000 description 1
- 238000003466 welding Methods 0.000 description 1
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- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
Abstract
一种葛根素的高效分离方法，采用超临界CO2流体为逆向萃取结晶溶剂，其通过乙醇－水混合溶剂溶解葛根素粗提取物，控制超临界流体结晶压力在10～30MPa范围，温度在50～70℃范围，结晶一次，葛根素纯度可由40％提高到80％以上。该处理过程，方法简便、成本低、结晶时间短、易于生产推广。A high-efficiency separation method of puerarin, which uses supercritical CO 2 fluid as the reverse extraction crystallization solvent, dissolves the crude puerarin extract through ethanol-water mixed solvent, controls the supercritical fluid crystallization pressure in the range of 10-30MPa, and the temperature at 50 In the range of ~70°C, once crystallized, the purity of puerarin can be increased from 40% to over 80%. The treatment process has the advantages of simple method, low cost, short crystallization time and easy production and popularization.
Description
One, technical field
The present invention relates to a kind of isolation and purification method and device of material, particularly effective ingredient crystallization purifying method and device in the kind of plant crude extract exactly are a kind of method and device of high efficiency separation of puerarin.
Two, background technology
Solution crystallization has important effect in the separating substances purge process; along with industrial expansion, efficient Crystallization Separation technology in the application in fields such as oil, chemical industry, biotechnology and environmental conservation more and more widely; the research of industrial crystallization technology and correlation theory also is pushed to the new stage, and the novel crystallization technology has obtained bigger progress both at home and abroad.
Crystallization is an important chemical process, and solute crystallizes out from solution and will experience two steps: nucleus generates and crystal growth.It is the nucleus that generates some in supersaturated solution that nucleus generates; And on the basis of nucleus, grow into crystal, then be crystal growth.The factor that influences whole crystallization process is a lot, as the speed of the existence of the degree of supersaturation of solution, impurity, stirring and various physical fields etc.In industrial crystallization, pursuing high crystal growth rate is in order to improve the production capacity of equipment, but in fact rate of crystalline growth is subjected to the restriction of problems such as desired specific product quality usually, do not allow to adopt the raising degree of supersaturation to improve growth rate, to under suitable degree of supersaturation, seek additive method and remove to promote crystal growing process.Crystalization in supercritical fluid separation method for example, Pan sees that professor waits the terpene lactones that once utilized supercritical fluid extraction Crystallization Separation method systematic study, supercritical fluid extraction Crystallization Separation method as bilobalide, andrographolide, and applied for national inventing patent (patent No.: ZL98 1 11546.2), (Pan sees also deeply systematically to have inquired into its mechanism of action, Zhang Wencheng, Xie Huiming etc. andrographolide is at supercritical CO
2Crystalline formation and growth [J] in the extraction. electron microscopic journal, 2003,22 (5): 377-379).
Because of micromolecule such as terpene lactones, anthraquinone class, low polar compound at supercritical CO
2In have certain dissolubility, relatively be fit to supercritical fluid extraction or extractive crystallization, and the chemical compound higher for molecular weight, that polarity is stronger, extract or extractive crystallization separates as this methods of very difficult employing such as flavonoid glycoside, Saponin classes.
Radix Puerariae has been prevented and cured diseases the existing long history of Herba indigoferae Pseudotinctoriae in the proved recipe as Chinese herbal medicine.Puerarin (the C that 80% (HPLC method) of present listing is above
20H
21O
9) product, its preparation technology mainly be with reference to Japanese bavin field hold two and side departure (the bavin field is held second-class: the chemical research of the research of oriental drug composition (the 1st newspaper) Radix Puerariae composition, pharmaceutical journal, 1959,79:755; Fang Qicheng etc.: the research of Radix Puerariae flavone, Chinese Medical Journal 1974, method 54:271) promptly uses ethanol extract with n-butyl alcohol-water liquid-liquid extraction, aluminium oxide decolouring, glacial acetic acid crystallization.The organic solvent that this technology is inflammable because of a large amount of uses, toxicity is big, the environmental pollution of production and the infringement of workers ' health have been caused, and cost height, production cycle is long, content of puerarin is low in the product, it is clean that the acetic acid that uses in the crystallization process is difficult to volatilization, makes that the impure height of its extract, solvent contamination are serious, poor stability, drug effect be low.
The extracting method of total flavones in the Radix Puerariae has been introduced in patent application 91110602.2 " kudzu vine root extract and preparation method and purposes ", and its purpose is to people provide a kind of medicine that can be used as anti-cancer, but products obtained therefrom purity is lower.
At present the preparation high purity puerarin mostly adopts macroporous resin adsorption to separate and organic solvent recrystallization or preparative hplc separation method, and its length consuming time, yield are low.
Three, summary of the invention
The present invention be directed to the existing experimentation for preparing the existing defective of method of high purity puerarin and carry out, be intended to simplify technology, raise the efficiency, provide a kind of for industrialized isolation and purification method.Technical problem to be solved is in specific device puerarin solution to be carried out Crystallization Separation with supercritical fluid.
The essence of the method for the high efficiency separation of puerarin that the present invention is alleged is the method that is prepared high purity puerarin by the puerarin crude extract, and this method is separated through primary crystallization, and puerarin content can be brought up to more than 80%.
The crystallization principle that technical scheme of the present invention is separated out according to solvent evaporates, solute at first is prepared into puerarin solution with the puerarin crude extract, in crystallization kettle this puerarin solution is carried out Crystallization Separation, it is characterized in that: adopt supercritical CO
2Fluid is just used supercritical CO as reverse extractive crystallization solvent
2Fluid extraction is also flung to solvent composition in the puerarin solution, and puerarin crystallization in solution is separated out.
Solvent should select it at supercritical CO
2Partition coefficient in the fluid is greater than the solvent of the partition coefficient of puerarin.Different solvents has different extracting pressure and temperature.Preferred puerarin ethanol water, 50～70 ℃ of extracting pressure 10～30MPa, extraction temperature are that 50～90% alcoholic solution is good as solvent with concentration.Experiment showed, that concentration of ethanol is that concentration expressed in percentage by weight or concentration expressed in percentage by volume influence are little, the size of puerarin concentration only influences the isolating time of extractive crystallization, has nothing to do with extracting pressure, temperature.
The device that this method is used comprises crystallization kettle 5, separating still 6, filter 7, compressor 8, heat exchanger 9 and CO
2Gas cylinder 10.This device is quite similar with supercritical fluid extraction equipment, be not both in the crystallization kettle 5 (extraction kettle that is equivalent to supercritical fluid extraction equipment) and be provided with crystallizer, crystallizer is used for adsorbing the puerarin crystal of separating out.Crystallizer is made up of the cylinder of two almost coaxials at least, and wherein the upper/lower terminal of urceolus 1 and still inner wall sealing are isolated still inwall and puerarin solution; The lower end of urceolus 1 and inner core 2 respectively is provided with annular plate washer 3, and annular plate washer 3 presss from both sides a sharp angle with barrel.Plate washer 3 is used for collecting the puerarin crystal that comes off on the barrel, and the fluidic effect of disturbance is arranged simultaneously.
For further disturbance fluid improves Crystallization Separation efficient, also disturbance ball 4 can be set at the entrance point of inner core 2.
The puerarin extractum of purity about 40% is mixed with puerarin solution with 50～90% ethanol, drops in the crystallization kettle, give heat and heat up, open CO
2Gas cylinder, 10～30MPa and 50～70 ℃ in the crystallization control still are closed gas cylinder, open pressure regulator valve, circulation Crystallization Separation 30min～100min.Puerarin is precipitating on crystallizer, and the second alcohol and water is recovered in separating still, crystallization once, drying can not obtain the puerarin product of purity more than 80%.
The present invention's supercritical CO
2It is obvious that the method that the crystallization of reverse extractant puerarin is separated out is separated with traditional macroporous resin adsorption, the solvent recrystallization method is compared advantage.Under the uniform temp condition, the separation and purification time of the inventive method is short, separation efficiency is high.The purity of the target product that primary crystallization precipitating of the present invention obtains is higher, is fit to the high efficiency separation purification of flavonoid glycoside, Saponin class material.This law technology is simple, need not complicated power consumption, technologies consuming time such as column chromatography.Its yield is also than the traditional handicraft height simultaneously, and 40% raw material is through primary crystallization, and puerarin purity surpasses 80%, and percent crystallization in massecuite 〉=35% is fit to produce in batches.
The crystalline rate of puerarin (%)=[quality of crystallized product (g) * content (%)] ÷ [applied sample amount (g) * content (%)] * 100%
Four, description of drawings
The process chart of this separation of Fig. 1 method
5 crystallization kettles, 6 separating still 7CO
2Gas cylinder 8 filters 9 compressors 10 heat exchangers
The cross-sectional view of Fig. 2 crystallizer
The outlet of 1 urceolus, 2 inner cores, 3 plate washers, 4 disturbance balls, 11 crystallization kettle imports, 12 crystallization kettles.
Five, the specific embodiment
(1), the method embodiment of high efficiency separation purified puerarin by utilizing is as follows:
Embodiment 1:
(1), to get 40% left and right sides puerarin crude product some, by the liquid-solid ratio ethanol of 5: 1 (v/m): water (90%: 10%) mixed solvent dissolves in 45 ℃ of water-baths;
(2), the puerarin mixed liquor of ethanol+water of preparing is positioned in the crystallization kettle, crystallization control pressure is 10MPa, and temperature is 70 ℃;
(3), through the 90min time, crystallization once, crystallized product;
(4), detect, can obtain 81.5% left and right sides puerarin, percent crystallization in massecuite is 37.0% through high performance liquid chromatography.
Embodiment 2:
(1), to get 40% left and right sides puerarin crude product some, by the liquid-solid ratio ethanol of 8: 1 (v/m): water (80%: 20%) mixed solvent dissolves in 45 ℃ of water-baths;
(2), the puerarin mixed liquor of ethanol+water of preparing is positioned in the crystallization kettle, crystallization control pressure is 15MPa, and temperature is 60 ℃;
(3), through the 90min time, crystallization once, crystallized product;
(4), detect, can obtain 82.7% right puerarin, percent crystallization in massecuite is 36.6% through high performance liquid chromatography.
Embodiment 3:
(1), to get 40% left and right sides puerarin crude product some, by the liquid-solid ratio ethanol of 10: 1 (v/m): water (90%: 10%) mixed solvent dissolves in 45 ℃ of water-baths;
(2), the puerarin mixed liquor of ethanol+water of preparing is positioned in the crystallization kettle, crystallization control pressure is 20MPa, and temperature is 55 ℃;
(3), through the 75min time, crystallization once, crystallized product;
(4), detect, can obtain 83.7% right puerarin, percent crystallization in massecuite is 37.5% through high performance liquid chromatography.
Embodiment 4:
(1), to get 40% left and right sides puerarin crude product some, by the liquid-solid ratio ethanol of 8: 1 (v/m): water (70%: 30%) mixed solvent dissolves in 45 ℃ of water-baths;
(2), the puerarin mixed liquor of ethanol+water of preparing is positioned in the crystallization kettle, crystallization control pressure is 25MPa, and temperature is 60 ℃;
(3), through the 45min time, crystallization once, crystallized product;
(4), detect, can obtain 82.2% right puerarin, percent crystallization in massecuite is 36.9% through high performance liquid chromatography.
Embodiment 5:
(1), to get 40% left and right sides puerarin crude product some, by the liquid-solid ratio ethanol of 5: 1 (v/m): water (50%: 50%) mixed solvent dissolves in 45 ℃ of water-baths;
(2), the puerarin mixed liquor of ethanol+water of preparing is positioned in the crystallization kettle, crystallization control pressure is 15MPa, and temperature is 65 ℃;
(3), through the 90min time, crystallization once, crystallized product;
(4), detect, can obtain 81.3% right puerarin, percent crystallization in massecuite is 36.3% through high performance liquid chromatography.
Embodiment 6:
(1), to get 40% left and right sides puerarin crude product some, by the liquid-solid ratio ethanol of 10: 1 (v/m): water (90%: 20%) mixed solvent dissolves in 45 ℃ of water-baths;
(2), the puerarin mixed liquor of ethanol+water of preparing is positioned in the crystallization kettle, crystallization control pressure is 10MPa, and temperature is 70 ℃;
(3), through the 90min time, crystallization once, crystallized product;
(4), detect, can obtain 80.3% right puerarin, percent crystallization in massecuite is 35.1% through high performance liquid chromatography.
(2) crystallizer
Get rustless steel processing urceolus 1, inner core 2 and annular plate washer 3.
The upper/lower terminal of urceolus 1 plays fixation simultaneously with politef and crystallization kettle inner wall sealing.Inner core 2 coaxial being fixed in the urceolus 1.
At the annular plate washer 3 of the inboard welding of urceolus 1 lower end barrel, make and barrel folder-sharp angle.
Weld annular plate washer 3 in both sides inside and outside the inner core 2 lower end barrels, make with barrel inside and outside both sides all accompany sharp angle.
Suspend a disturbance ball 4 in midair in inner core 2 lower ends with stainless steel silk on axial location, disturbance ball 4 is hollow stainless steel ball.
Claims (5)
1, a kind of method of high efficiency separation of puerarin is in crystallization kettle puerarin solution to be carried out Crystallization Separation, it is characterized in that: use supercritical CO
2Fluid extraction is also flung to solvent in the puerarin solution, and puerarin crystallization in solution is separated out.
2, the method for high efficiency separation of puerarin according to claim 1 is characterized in that: use supercritical CO
2Fluid carries out Crystallization Separation to the puerarin ethanol water, 50～70 ℃ of extracting pressure 10～30MPa, extraction temperature.
3, the separation of high efficiency separation of puerarin according to claim 1 and 2 is characterized in that: described puerarin ethanol water is that puerarin is dissolved in the solution that forms in 50～90% the ethanol.
4, the device of being used by the described separation method of claim 1 comprises crystallization kettle (5), separating still (6), filter (7), compressor (8), heat exchanger (9) and CO
2Gas cylinder (10), it is characterized in that: in crystallization kettle (5), crystallizer is arranged, crystallizer is made up of the urceolus (1) and the inner core (2) of two almost coaxials at least, urceolus (1) upper/lower terminal and still inner wall sealing, the lower end of urceolus (1) and inner core (2) respectively is provided with annular plate washer (3), and plate washer (3) presss from both sides a sharp angle with barrel.
5, device according to claim 4 is characterized in that: the entrance point at inner core (2) is provided with disturbance ball (4).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CNB200610088090XA CN100375624C (en) | 2006-06-23 | 2006-06-23 | Method and device for high efficiency separation of puerarin |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB200610088090XA CN100375624C (en) | 2006-06-23 | 2006-06-23 | Method and device for high efficiency separation of puerarin |
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| CN1868484A true CN1868484A (en) | 2006-11-29 |
| CN100375624C CN100375624C (en) | 2008-03-19 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20120130066A1 (en) * | 2010-11-22 | 2012-05-24 | The Hong Kong Polytechnic University | Method for precipitating a solute from a solution |
| CN103204848A (en) * | 2012-01-11 | 2013-07-17 | 随州市康汇保健品有限公司 | Method for extracting puerarin |
| CN104844584A (en) * | 2015-04-17 | 2015-08-19 | 荆楚理工学院 | Method for extracting puerarin from residue of radix puerariae |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1276378A (en) * | 1999-06-03 | 2000-12-13 | 西北大学 | Process for extracting high-purity kakonein for medicine |
| CN1233652C (en) * | 2004-03-23 | 2005-12-28 | 合肥工业大学 | Crystallizing and purifying Method for preparing puerarin in high purification |
-
2006
- 2006-06-23 CN CNB200610088090XA patent/CN100375624C/en not_active Expired - Fee Related
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20120130066A1 (en) * | 2010-11-22 | 2012-05-24 | The Hong Kong Polytechnic University | Method for precipitating a solute from a solution |
| US9138705B2 (en) * | 2010-11-22 | 2015-09-22 | Hong Kong Polytechnic University | Method for precipitating a solute from a solution |
| CN103204848A (en) * | 2012-01-11 | 2013-07-17 | 随州市康汇保健品有限公司 | Method for extracting puerarin |
| CN104844584A (en) * | 2015-04-17 | 2015-08-19 | 荆楚理工学院 | Method for extracting puerarin from residue of radix puerariae |
| CN104844584B (en) * | 2015-04-17 | 2017-07-28 | 荆楚理工学院 | A kind of method that Puerarin is extracted in Pueraria lobota slag |
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| Publication number | Publication date |
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| CN100375624C (en) | 2008-03-19 |
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