CN1862260B - Liquid delivery device and analysis system - Google Patents

Abstract

The problem to be solved by the present invention is that, when a conventional liquid conveying device filled with oil operates a plurality of liquids, the flow of oil generated by its operation affects other liquids. The technical solution of the present invention is that, in the liquid delivery device, the delivery liquid is surrounded by air-separated oil droplets for operation. In addition, as a liquid supply method in which a plurality of liquids are successively surrounded and treated with oil droplets, one oil droplet is supplied corresponding to one liquid droplet from the liquid introduction part to generate a liquid surrounded by oil droplets. According to the present invention, even when handling a plurality of liquids, it is possible to operate stably without affecting other liquids. As a liquid continuously surrounded by oil droplets, it can be handled in the liquid delivery device and easily applied in the analysis system.

Description

Liquid delivery device and analysis system

technical field

The present invention relates to systems for manipulating liquids on substrates. It is especially concerned with analytical or reaction systems where many liquids are transported.

Background technique

As an analysis device for detecting the amount of components contained in a sample, a spectroscopic analysis device is widely used, which is to irradiate white light emitted from a halogen lamp etc. The grating splits the light transmitted through the reaction solution, extracts necessary wavelength components, and calculates the absorbance to measure the amount of the target component. Alternatively, white light may be irradiated onto the reaction solution after being separated by a diffraction grating. In these analytical devices, conventionally, a sample and a reagent are dispensed into a plastic or glass reaction container, and they are mixed to form a reaction solution, which is irradiated with light to measure the amount of the component.

However, in recent years, in order to reduce the cost of reagents and reduce the load on the environment, it is required to reduce the amount of the reaction solution used in the analysis, and there is a problem that the operation of the reaction solution is changed when the amount of the reaction solution is reduced by the conventional method. It is difficult to obtain, and it cannot be accurately measured due to air bubbles generated during dispensing and mixing. Therefore, techniques for correctly handling minute amounts of liquid are required.

As one method of handling a minute amount of liquid, there is a method of electrically controlling the liquid transported on electrodes formed on a planar substrate. One of the representatives of this method is, by forming the liquid that is transported into a granular liquid between two opposing substrates with a plurality of electrodes and sandwiching it, the electrodes disposed along the surface between the two opposing substrates The electrodes apply a voltage, thereby transporting the liquid. (For example, Non-Patent Document 1-Applied Pyhysics Letters, Vol, 77, No.11, pp.1725-1726, 2000, Non-Patent Document 2-IEEE15th, Int. Conf. MEMS Jan 2002, p.97-100.). A device consisting of two opposing substrates is referred to as a liquid transport device. For this method, generally, a plurality of electrodes are formed on one substrate along a liquid delivery path through which the liquid is delivered, and an electrode connected to the ground is also provided on the other substrate. When a voltage is applied to an electrode in the lower part of the granular liquid, according to the electrowetting phenomenon (for example, non-patent document 3-Polymr 37 (1996) 2465-2470), the wettability on the electrode to which the voltage is applied becomes good, which The granular liquid moves to be placed on the electrode to which the voltage is applied. The fluid is delivered by doing this repeatedly.

In addition, it is also reported that liquid is distributed to branches using an electrode array having a plurality of branches, or that liquids are merged at a position where a plurality of grooves join (for example, Patent Document 1 - Japanese Patent Application Laid-Open No. 10-267801). In addition, a case where a single granular liquid is divided is also reported (for example, Patent Document 2 - US Patent Publication No. 6565727). In addition, a system that transports a sample and detects it inside a liquid transport device has also been reported (for example, non-patent document 4-Micro Total Analysis Systems 2003 p.1287-1290).

Advantages of such a liquid transport device for transporting liquid include that, compared with a container surrounded by walls, it is less affected by air bubbles due to the use of a substrate. Here, there are two kinds of reports on the media filling the interior of the liquid delivery device. One is a case where the inside is filled with oil as described in Non-Patent Document 1, and the other is a case where the inside is filled with air as described in Non-Patent Document 2.

On the other hand, as a liquid transport method for an automatic analyzer, there is a report of introducing and transporting an aqueous solution segment into a conduit containing a liquid such as silicone oil (for example, Patent Document 3 - US Patent Publication No. 3479141). With regard to devices for aspirating, dispensing separated liquids, or conveying in a liquid stream, there are reports of delivering multiple sample segments with air segments and immiscible liquid segments spaced apart from each other (for example, Patent Document 4 - U.S. Patent Gazette Bulletin No. 4259291).

Moreover, there is also a liquid surrounded by oil droplets, which is transported by electrowetting (non-patent literature 5-Lab-on-a-Chip: Platforms Devices, and Applications, Conf.5591, SPIEO Ptics East, Philadelphia, Oct.25 -28, 2004) report.

In the case of filling the inside of the liquid delivery device with oil, since the conveyed granular liquid is surrounded by oil, the surface with the liquid delivery device is less likely to be polluted, the friction between the liquid and the liquid delivery device is reduced by the oil and the movement required Voltage drop, can prevent the evaporation of liquid and other advantages. However, it is necessary to seal the bottom surface and the side surface of the liquid conveying device so that oil does not leak, and installation is also labor-intensive. The problem to be considered is that when the liquid is manipulated, the oil will flow and other liquids will be affected. For example, when a liquid is introduced into a liquid delivery device, problems to be considered are that the measuring tube for dispensing the liquid and the introduced granular liquid flow in the oil and move other liquids arranged in the liquid delivery device. Where the liquid moves uncontrollably, there is a possibility that it will come into contact with and mix with other liquids or that the liquid will break away from the liquid delivery channel and cannot be delivered. Especially in an analysis system, it is necessary to perform many operations for various liquids, such as dispensing, transporting, mixing, detecting, and discharging, for many samples and reagents. Therefore, it is important that operations on one liquid do not affect other liquids.

In addition, when considering application to an analysis system for detection in a liquid transport device, there is a problem that when air bubbles enter the oil from an inlet for introducing liquid, the air bubbles interfere with measurement in the measurement section. On the contrary, in the case of filling the liquid delivery device with air, since there is no need to seal the bottom and side surfaces of the liquid delivery device, installation is easy; and since the liquid is surrounded by air, the operation of a certain liquid will not Effects on other liquids have the advantage of being able to operate independently and stably on many liquids. However, unlike the case of being surrounded by oil, since the liquid directly contacts the surface of the liquid delivery device, it is considered that the internal components of the liquid are easily adsorbed, or that the friction between the liquid and the liquid delivery device is relatively large and causes movement. The required voltage increases, and evaporation of the liquid, etc., cannot be prevented. In an analysis system using a liquid transport device, in order to measure the concentration of a component in a sample as a liquid to be transported, it is important to prevent adsorption and evaporation of components in the liquid.

In an embodiment in which the liquid to be transported is surrounded by droplets of the liquid for transport that do not mix with the liquid to be transported, and the liquid for transport is transported together with the liquid to be transported, it is necessary to surround the liquid for transport appropriately. Although it is necessary for a general analysis system to continuously detect multiple samples, when using this transport method to detect multiple samples, in order to prevent contamination of the sample and improve the efficiency of detection, it is necessary to properly and easily perform this encirclement. .

Contents of the invention

The object of the present invention is to provide a device that has both the advantages of filling with oil and filling with air, is easy to install, can stably transport many liquids, is difficult to absorb the components inside the liquid, and can reduce the voltage required for movement. , and a liquid delivery device that can prevent the sample from evaporating, and an analysis system that continuously transports many samples together with the delivery liquid.

The liquid transporting device provided by the present invention adopts separate transporting liquids to surround the transporting liquid, and together with the transporting liquid, transports the transporting liquid. Therefore, it is easy to install, can transport multiple liquids stably, and the components inside the liquid are difficult to absorb, so that the voltage required for movement can be reduced, and the evaporation of the sample can be prevented. The analysis system provided by the present invention sequentially introduces the transported liquid into the separated transported liquid which is not mixed with the transported liquid, so as to continuously transport the transported liquid together with the transported liquid.

As an example of an analysis system, it has: a first unit provided with a part for supplying the first liquid and a part for supplying the second liquid; a first introduction part for introducing the first liquid from the part for supplying the first liquid; In the discharge part that discharges the above-mentioned first liquid, there is a liquid delivery channel that has a plurality of electrodes and connects the above-mentioned introduction part and the above-mentioned discharge part, a measurement part provided on at least a part of the above-mentioned liquid delivery channel, and at least a part of the plurality of electrodes. A second unit of a voltage applying member for applying a voltage; a third unit having a detection system for detecting the above-mentioned measuring part; a fourth unit for discharging liquid from the above-mentioned discharge part; it is characterized in that the above-mentioned second liquid is the same as the above-mentioned first If the liquids are immiscible, the first unit divides the second liquid and supplies it to the second unit so that the separated first liquid is contained therein, and the voltage applying part applies a voltage to at least a part of the plurality of electrodes, so that A complex of the divided first liquid and the divided second liquid including the divided first liquid moves along the liquid transport path.

As another example of the analysis system, it has: a first unit having a part for supplying the first liquid and a part for supplying the second liquid; a first introduction part for introducing the first liquid from the part for supplying the first liquid, A second introduction part for introducing the second liquid from the part for supplying the second liquid, a discharge part for discharging the first liquid, a liquid delivery channel having a plurality of electrodes and connecting the first introduction part and the discharge part, A second unit comprising a measurement unit provided on at least a part of the liquid transport channel and a voltage applying member for applying a voltage to at least a part of the plurality of electrodes; a third unit including a detection system disposed adjacent to the measurement unit; A fourth unit for discharging liquid from the discharge part; characterized in that the second liquid is immiscible with the first liquid, and the part for supplying the first liquid separately sends the first liquid and supplies the second liquid. The liquid part sends out the second liquid separately, and the voltage applying part applies a voltage to at least a part of the plurality of electrodes, so that the first liquid separated by the part for supplying the first liquid flows along the liquid from the first introduction part. The transfer channel moves toward the second introduction part, and moves the first liquid contained in at least a part of the second liquid introduced into the second introduction part along the liquid transfer channel.

If the present invention is used, compared with the previous liquid delivery device filled with oil, it is easy to install, the liquid will not move uncontrollably due to the oil flow generated by the liquid operation such as dispensing and transportation, and it can stably and independently carry out many liquids. operation. In addition, the liquid surrounded by the separated oil droplets can be continuously introduced into the liquid delivery device, which can improve the efficiency of an analysis system that processes many samples.

Description of drawings

Fig. 1 is a sectional view showing a liquid transfer channel of a liquid transfer device according to Embodiment 1 of the present invention.

Fig. 2 is a schematic diagram illustrating the liquid delivery sequence in Embodiment 1 of the present invention.

Fig. 3 is a cross-sectional view showing another structure of a liquid transfer channel according to Embodiment 1 of the present invention.

Fig. 4 is a cross-sectional view showing another structure of a liquid transfer channel according to Embodiment 1 of the present invention.

Fig. 5 is a cross-sectional view showing another structure of a liquid transfer channel according to Embodiment 1 of the present invention.

Fig. 6 is a cross-sectional view showing another structure of a liquid transfer channel according to Embodiment 1 of the present invention.

Fig. 7 is a graph showing the speed comparison in the liquid transport device of the present invention.

Fig. 8 is an explanatory diagram for transporting a liquid containing substances present in oil of Example 1 of the present invention.

Fig. 9 is a schematic diagram illustrating Embodiment 2 of the present invention.

Fig. 10 is a schematic diagram illustrating Embodiment 2 of the present invention.

Fig. 11 is a schematic diagram illustrating Embodiment 2 of the present invention.

Fig. 12 is a schematic diagram illustrating Embodiment 2 of the present invention.

Fig. 13 is a schematic diagram illustrating Embodiment 3 of the present invention.

Fig. 14 is a schematic diagram illustrating Embodiment 4 of the present invention.

Fig. 15 is a schematic diagram illustrating Embodiment 4 of the present invention.

Fig. 16 is a schematic diagram illustrating an analysis system of Embodiment 5 of the present invention.

Fig. 17 is a diagram showing the sequence of operations in the liquid transport device when the analysis system of the present invention is used.

Fig. 18 is a layout diagram of various parts in the liquid delivery device of the present invention.

Fig. 19 is an external view of the liquid delivery device of the present invention.

Fig. 20 is a schematic diagram of the control system of the present invention.

Fig. 21 is a cross-sectional view of a sample introduction port in Example 5 of the present invention.

Fig. 22 is a cross-sectional view of a sample introduction port in Example 5 of the present invention.

Fig. 23 is a sectional view of a reagent inlet in Example 5 of the present invention.

Fig. 24 is a cross-sectional view of a measurement unit in Example 5 of the present invention.

Fig. 25 is a cross-sectional view of a measurement unit in Example 5 of the present invention.

Fig. 26 is a sectional view of a discharge port according to Embodiment 5 of the present invention.

Fig. 27 is a cross-sectional view of a discharge port according to Embodiment 5 of the present invention.

Fig. 28 is a schematic diagram illustrating Embodiment 6 of the present invention.

Fig. 29 is a schematic diagram illustrating Embodiment 5 of the present invention.

Fig. 30 is a schematic diagram illustrating Embodiment 7 of the present invention.

Fig. 31 is a schematic diagram illustrating Embodiment 7 of the present invention.

Detailed ways

Example 1

This embodiment shows a procedure for transporting a liquid surrounded by oil droplets using oil droplets as droplets of the transport liquid in the liquid transport device. FIG. 1 shows a cross-sectional view of the liquid delivery channel of the liquid delivery device 10 of this embodiment. The liquid transport device 10 is composed of a lower substrate 8 and an upper substrate 9 . The lower substrate 8 is provided with a plurality of control electrodes 5 on the upper surface of the insulating substrate 4 along the conveying direction of the liquid (oil droplet) 1, and its surface is covered with an insulating film 7. The upper substrate 9 is provided with a common electrode 6 on the lower surface of the insulating substrate 4', and its surface is covered with an insulating film 7'. Furthermore, the surfaces of the respective insulating films 7, 7' are provided with hydrophobic properties to facilitate liquid transport, and hydrophobic films 100, 100' are applied to the surfaces. The transported liquid 1 is arranged between the two substrates, surrounded by oil droplets 2 . That is, the transported liquid 1 is surrounded by the oil droplet 2 and becomes a complex of the liquid droplet of the liquid 1 and the oil droplet 2 . Here, being enclosed means that one oil droplet is at a position substantially covering the outer surface of one liquid droplet (liquid). Air 3 exists around the oil droplet 2 .

In this embodiment, the insulating substrates 4, 4' are made of quartz, the control electrode 5 and the common electrode 6 are made of indium-tin oxide (ITO-Indium-TinOxide), and the insulating films 7, 7' are made of chemical vapor deposition ( CVD-Chemi Vapor Deposition) film-formed SiO ₂ , and CYTOP (registered trademark) manufactured by Asahi Glass Co., Ltd. was used as the hydrophobic film. The thickness of ITO was 70 nm, and the thickness of the insulating film 7 formed by CVD was 200 nm. In addition, the distance between the lower substrate 8 and the upper substrate 9 was set to 0.5 mm. A 0.9 wt% NaCl solution was used as the liquid 1, and silicone oil was used as the oil droplet 2, and the liquid volumes were 5 μL each. By providing the liquid surrounded by oil droplets between the lower substrate 8 and the upper substrate 9, the effect of preventing the oil from evaporating is improved, and the oil is prevented from being separated from the liquid by gravity.

Fig. 2 shows a perspective view from above of the liquid delivery device used in this embodiment. For the sake of simplification and ease of understanding, in this application, only the liquid 1, the oil drop 2, and the control electrode 5 are shown in the perspective views of Fig. 2 and Fig. 8-Fig. The common electrode 6 located above the control electrode 5 is connected to the ground, and a voltage is applied between the common electrode 6 and a part of the control electrodes 5 . In this application, the control electrode 5 to which no voltage is applied is in a floating state without being connected to any place. When the applied voltage is cut off, once the control electrode 5 is connected to the ground after the voltage application is stopped, the control electrode 5 is in a floating state. . Therefore, when the applied voltage is turned off, it is possible to prevent the insulating film and the hydrophobic film on the control electrode from becoming partially charged. When a voltage is applied between the common electrode 6 and the control electrode 5, the liquid 1 having a region partially overlapping the control electrode 5 moves and is placed on the control electrode 5 to which the voltage is applied, i.e. , the liquid moves as if placed on the control electrode 5 to which the voltage is applied. At the beginning, as shown in Figure 2 (a), a plurality of control electrodes 5 are arranged along the liquid delivery channel, and when there is liquid 1 surrounded by oil droplets 2 near the control electrode 5b, a voltage is applied to the control electrode 5b, as shown in Figure 2 As shown in (b), the liquid 1 is moved so as to be placed on the control electrode 5b.

Next, when a voltage is applied to the control electrode 5c and the voltage applied to the control electrode 5b is cut off, as shown in FIG. The gas-liquid interface with the air 3 moves together with the oil droplet 2 as shown in FIG. 2( d ), and is placed on the control electrode 5c. Furthermore, when a voltage is applied to the control electrode 5d and the applied voltage of the control electrode 5c is cut off, the liquid 1 squeezes the gas-liquid interface between the oil droplet 2 and the air 3 as shown in FIG. It moves together with the oil droplet 2 and moves onto the control electrode 5d. Through such repetition, the liquid 1 can be transported while being surrounded by the oil droplets 2 . And, the liquid 1 surrounded by the oil droplets 2 equals to move along the arrangement of the control electrodes. As shown in this embodiment, by surrounding the liquid with the liquid for transfer, adsorption of internal components of the liquid to be transferred to the surface of the substrate can be prevented, and evaporation of the liquid can be prevented. Furthermore, since the friction between the liquid 1 and the liquid transport device 10 is small, the liquid can be transported even at a low voltage compared to the case where the liquid transport device is filled with air. When the voltage applied to the control electrode is too high, it is useful to reduce the voltage because there is a risk of destroying the insulating film. Since the oil droplet 2 is used as the transport liquid and is separated, the oil flow caused by the transport liquid 1 will not be transmitted to other liquids, and many liquids in the liquid transport device can be stably and independently operated.

In this embodiment, NaCl solution was used as the liquid, but ionic liquids such as pure water and buffer solutions may also be used. It can also be blood and solutions containing DNA. It is also possible to contain latex particles, cells, magnetic beads in the liquid. Although silicone oil is used as the oil droplet 2, it may be a liquid that does not mix with the liquid to be transported, such as vegetable oil, paraffin oil, Krytox (registered trademark) manufactured by DuPont, or a fluorine-based solvent. liquid. Quartz is used as the insulating substrates 4, 4', but they may be substrates in which an insulating film such as an oxide film is formed on a conductive substrate such as Si or a resinous substrate. The insulating films 7 and 7' are made of SiO ₂ formed by CVD, but insulating films such as polysilazane, SiN, and parylene may be used. As the hydrophobic membranes 100 and 100', CYTOP manufactured by Asahi Glass Co., Ltd. is used, but TeflonAF (registered trademark) manufactured by DuPont Co., silicon-based hydrophobic membranes, or CF (Fluorocarbon film) membranes formed by CVD may also be used. ).

In this embodiment, although the hydrophobic film is formed on the insulating film, it may be a hydrophobic insulating film or an insulating hydrophobic film. In this case, since the number of steps is reduced, device fabrication becomes easier. In this embodiment, one common electrode 6 is provided, but a plurality of common electrodes 6 may be arranged on the upper substrate 9 as shown in FIG. 3 . In this case, there is an effect of improving the positional accuracy of the liquid during transportation. In this embodiment, although the control electrode 5 and the common electrode 6 are covered with insulating films 7 and 7' respectively, as shown in FIG. There is no insulating film 7'. In this case, a simple structure can be produced. In addition, the surface of the common electrode 6 may not be coated with the hydrophobic film 100, 100'. In this case, a simpler structure can be produced.

As shown in Figure 5, the common electrode 6 etc. are arranged between the control electrodes 5 of the lower substrate 8 in advance, the common electrode 6 is not arranged on the surface of the upper substrate 9, and a plurality of electrodes are provided on the lower substrate 8, then Any one of the plurality of electrodes may be used as the control electrode 5 , and any other one may be used as the common electrode 6 . In this case, since there is no need to form an electrode film on the upper substrate, the structure is simpler. Furthermore, the surface of the insulating substrate 4' of the upper substrate 9 may not be coated with a hydrophobic film. In this case, a simpler structure can be made. In addition, as shown in FIG. 6, the upper substrate 9 may not be provided, and only the lower substrate 8 may be used for transportation. The structure at this time is further simplified.

In this embodiment, although the lower substrate 8 and the upper substrate 9 constituting the liquid transport device 10 are arranged horizontally, it is also possible to make a part or the entire surface of the lower substrate 8 and the upper substrate 9 parallel to the horizontal surface. The directions are arranged at an angle. In the previous liquid conveying device filled with oil, when the angle is formed with the horizontal direction, there will be oil leakage, but if the present invention is used, since the oil is held by two horizontal substrates, the oil is difficult to flow, and it can be used with The horizontal direction forms an angle. If an angle can be formed with the horizontal direction, the installation area of the entire device can be reduced, which is useful. In addition, although silicone oil is used to surround the liquid 1 in this embodiment, it is also possible to further surround the silicone oil with a fluorine-based oil or a fluorine-based solvent.

Next, the transport speed of the oil droplet transported while the liquid 1 surrounded by the oil droplet 2 is pressed against the gas-liquid interface of the oil droplet 2 and the transport speed in the air when the surrounding of the liquid 1 is air are compared. Fig. 7 shows the speed change of the liquid 1 with respect to the voltage change in various cases. In the case of oil droplet delivery, the liquid can be delivered from 20V, but in the case of air delivery, the liquid cannot be delivered at 20V, and the liquid can be delivered from 40V, so it can be seen that the voltage required for delivery can be reduced. This is considered to be due to the fact that oil is added between the liquid 1 and the surface of the liquid delivery device to reduce the friction. When the voltage applied to the electrodes is too high, it is useful to lower the voltage used to transport the liquid because there is a risk of breaking the insulating film.

The case where the substance contained in the oil droplet is transported simultaneously with the liquid will be described using FIG. 8 . As shown in Figure 8(a), when there are two liquids (droplets, hereinafter the same) 1a, 1b in one oil droplet 2 in the liquid delivery channel, as shown in Figure 8(b), when the control electrode 5e When a voltage is applied, only the liquid 1b which has an overlapping area with the control electrode 5e moves onto the control electrode 5e. At this time, the liquid 1a squeezes the gas-liquid interface of the oil and air of the oil droplet 2, and is transported together with the liquid 1b as shown in FIG. 8(c). Furthermore, as shown in Fig. 8(d), when a voltage is applied to the control electrode 5f and the voltage applied to the control electrode 5e is cut off, the liquid 1b moves to the control electrode 5f, and the liquid 1a also squeezes the gas-liquid interface between oil and air and move. The same movement is also possible when there are two or more liquids in the oil droplet 2 . As described above, when there are a plurality of liquids (droplets) in the oil droplet 2, the liquid droplets other than the voltage-applied droplet can also move together by pressing the gas-liquid interface of the oil droplet. Therefore, when two or more liquids are simultaneously transported, it is only necessary to control one liquid by surrounding them with the same oil droplet, and the control of the liquid becomes easy.

In this embodiment, as the substance in the oil droplet 2, a liquid different from the liquid to which the voltage is applied is used as an example, but any substance contained in the oil droplet 2 is sufficient, and even a solid such as a particle can move in the same way. . Normally, oil cannot be transported in a controlled manner in a liquid transport device, but with the present invention, oil can be moved to a predetermined position by transporting a liquid contained in oil as oil droplets. Therefore, by moving and mixing many oils, the substances contained in the oils can also be reacted.

Example 2

This example shows the sequence of mixing two liquids, etc., surrounded by oil droplets. The following is divided into combination (synthesis) and stirring to explain the mixing order.

As shown in Figure 9 (a), when the liquid (droplet) 1a surrounded by the oil droplet 2a and the liquid 1b surrounded by the oil droplet 2b are disposed on the control electrode 5, they are positioned between the two liquids (droplets) and each When the control voltage is applied to the control electrodes 5a, 5b partially overlapping the droplets, the liquids (droplets) 1a, 1b are moved to the respective partially overlapping control electrodes as shown in FIG. 9(b). Next, the control electrodes arranged between the two liquids (droplets) as shown in FIG. The voltage is applied to the electrode 5c, and the voltage applied to the control electrodes 5b and 5d which are control electrodes adjacent to the control electrode 5c is cut off. Therefore, the droplets of the two liquids will move to the control electrode 5c; after the oil 2a and the oil 2b are combined as shown in Figure 9(d), the liquid 1a and the liquid 1b will be combined as shown in Figure 9(e) to become an oil Surrounded by liquid 1. Since the combined liquid is surrounded by oil droplets as in this embodiment, the flow of oil generated by the combination does not spread to liquids other than the combined liquid, and many liquids in the liquid delivery device can be stably and independently operated.

Next, the procedure for stirring the liquid component surrounded by oil droplets is shown. FIG. 10 shows an arrangement diagram of control electrodes for stirring the liquid 1 . As shown in FIG. 10, when the liquid 1 in the oil droplet 2 is located on a plurality of electrodes (indicated by squares in the figure) arranged substantially in a straight line, a voltage is sequentially applied to the control electrodes 5, and the liquid 1 can be stirred by reciprocating movement. The ingredients inside. Moreover, as shown in FIG. 11, even if the control electrodes are arranged on a plurality of substantially straight lines in different directions, and the substantially straight lines are crossed, by repeatedly moving the liquid 1 back and forth in two different directions, it is possible to perform Stir. At this time, diffusion is facilitated by deforming part of the liquid by sending it in different directions. As shown in FIG. 12 , by applying a voltage to control electrodes along the moving direction of the liquid 1 on the control electrodes arranged in a two-dimensional array, the moving direction of the liquid 1 can also be two-dimensionally controlled and stirred. For example, even if the liquid 1 is moved like a circle, it can be stirred. In this case, during the movement of the liquid in a circular manner, parts of the liquid deform, promoting diffusion. At this time, if the size of the oil droplet 2 is large, the liquid 1 moves inside the oil droplet without contacting the gas-liquid interface between the oil and the gas, and can be stirred more quickly.

As in the present embodiment, by surrounding the stirring liquid with oil droplets, even if the liquid is moved for stirring, the flow of the oil is not transmitted to other liquids, so it is possible to stably control liquids other than the stirring liquid. By performing the mixing operation of merging and stirring in the above oil droplets, the flow of oil generated by the mixing operation is not transmitted to other liquids, and many liquids in the liquid delivery device can be stably and independently operated. Such a mixing operation is important in analysis. For example, in order to measure the total protein (TP-Total Protein) item, which is a biochemical analysis item for measuring the total protein amount contained in blood, by mixing 1 μL of blood in the sample, It can be obtained by mixing 10 μL of Otocella TP reagent manufactured by Daiichi Chemicals Co., Ltd. with the reagent, and measuring the absorbance 10 minutes later.

Example 3

This embodiment shows the sequence of separating one liquid 1 surrounded by oil droplets 2 into two liquids 1 surrounded by oil droplets 2 .

When the liquid 1 surrounded by oil droplets 2 is placed on the control electrode 5 arranged in a straight line as shown in FIG. 13( a), a part of the liquid 1 overlaps as shown in FIG. The nearby control electrode 5c, as well as the control electrodes 5b and 5d adjacent to the control electrode located near the center of the liquid and symmetrically positioned with respect to this control electrode, are simultaneously or substantially simultaneously voltage applied. In this way, the liquid is deformed so as to become elongated laterally as shown in FIG. 13(c). Next, the voltage applied to the control electrode 5c located near the center of the liquid is cut off. In this way, as shown in Fig. 13(d), two liquids (droplets) 1a, 1b are separated. At this time, each of the control electrode 5a arranged earlier than the control electrode 5b in the direction toward one end of the control electrode row and the control electrode 5e arranged earlier than the control electrode 5d in the direction toward the other end of the control electrode row By cutting off the applied voltage of the control electrodes 5b and 5d, as shown in FIG. drops) 1b. As shown in this embodiment, by separating two liquids in oil droplets, oil flow due to the separation operation does not affect liquids other than the separated liquid, and many liquids in the liquid delivery device can be stably and independently operated.

Example 4

This example shows the sequence of generating liquid surrounded by oil droplets 2a within the liquid delivery device.

The liquid 1 and the oil droplet 2 are individually arranged on the control electrode 5 arranged linearly as shown in FIG. 14( a ). Here, in a plurality of control electrode rows, when a voltage is applied to the control electrode 5b arranged in the direction of the electrode where the center of the oil droplet 2 is located, as seen from the electrode where the center of the liquid 1 is located, as shown in Figure 14 (b) The liquid 1 then moves onto the control electrode 5b. Next, as shown in FIG. 14( c), the control electrode which is the destination of the liquid 1 is arranged in the direction toward the electrode at the center of the oil droplet 2, and is located at the electrode at the center of the oil droplet 2. A voltage is applied to the control electrode 5c arranged in the direction of the electrode located in the center of the liquid 1, and the voltage applied to the control electrode 5b is cut off. At this time, when the liquid droplet 1 comes into contact with the oil droplet 2, the oil droplet 2 moves as shown in FIG. 14(d) and surrounds the entire liquid 1, whereby the liquid 1 surrounded by the oil droplet can be produced. As shown in this example, a liquid surrounded by oil droplets can be easily generated by delivering a liquid within a liquid delivery device and bringing it into contact with oil. For example, when analyzing with a liquid delivery device, by separating the sample into a plurality of liquids like this embodiment in the liquid delivery device, and measuring them separately, many items can be measured at the same time, which has the effect of improving efficiency. Effect.

According to the physical range or the state of hydrophilicity and hydrophobicity of the surface, it shows the order in which the liquid (droplet) surrounded by oil droplets is generated from the oil buffer accumulated in a part of the liquid transport device. When the liquid 1 and the oil region 11 formed as an oil pool are individually arranged on the control electrodes 5a, 5b, 5c, 5d, 5e, and 5f arranged substantially in a straight line as shown in FIG. 15(a), the control electrode 5b is applied When the voltage is applied, the liquid 1 moves to the control electrode 5b as shown in Fig. 15(b). At this time, a part of the liquid 1 overlaps with the region where the oil region 11 is located. Next, as shown in FIG. 15(c), a voltage is applied to the control electrode 5c, and the voltage applied to the control electrode 5b is cut off. Then, the liquid 1 moves to the control electrode 5c, completely surrounds the liquid region 11, and then applies a voltage to the control electrode 5d as shown in FIG. 15(d), and then moves to the control electrode 5d.

Next, as shown in Fig. 15(e), a voltage is applied to the control electrode 5e, and the voltage of the control electrode 5d is cut off. The liquid 1 is then moved to the outside of the oil zone 11 . At this time, although a part of the oil is still in contact with the oil region 11, a certain amount of oil is separated from the oil region 11 by further applying a voltage to the control electrode 5f as shown in FIG. A liquid 1 surrounded by oil droplets 2 can be produced. In addition, by optimizing the range of the oil region 11, the surface state of hydrophilicity and hydrophobicity, and the electrode shape, etc., oil can be separated from the oil region and produced more easily. As shown in this example, the liquid surrounded by the oil can be easily generated by sending the liquid to the oil area where the oil is accumulated and bringing it into contact. By continuously feeding liquid to the oil area, it is possible to use one oil area to generate several oil-surrounded liquids.

Example 5

This embodiment shows that the liquid delivery device is applied to the analysis system, and the sample and the reagent are respectively introduced into a region in the liquid delivery device as a granular liquid, that is, as a droplet, as a region surrounded by a delivery liquid, that is, an oil droplet. Oil droplets and samples or composite droplets composed of oil droplets and reagents are transported, the composite droplets are combined and used as a reaction solution, stirred, detected, and discharged together with surrounding oil droplets.

Fig. 16 shows the structure of the analysis system. The analysis system has the following parts: a liquid delivery device 10 for dispensing a sample 21 into the liquid delivery device 10; a sample unit 12 for dispensing a reagent 25 contained in a reagent tank into the liquid delivery device 10 The reagent unit 13 is used to detect the sample 21 contained in the sample tank, for example, the detection unit 14 for measuring the internal components of the sample 21 and the discharge unit for discharging the sample 21, the reagent 25 and the oil droplet 2 15. A sample 21 and an oil 22 are arranged in the sample unit 12, and can be introduced into the liquid transport device 10 from one area, that is, the sample inlet 23, by the sample tube 16 and the oil tube 17, respectively.

Reagent 25 and oil 22 are arranged in reagent unit 13 , and can be introduced into liquid delivery device 10 from one area, that is, reagent inlet 24 , by reagent tube 18 and oil tube 17 , respectively.

The detection unit 14 is provided adjacent to the measurement unit provided on at least a part of the liquid transport channel connecting the sample or the like introduction unit and the discharge unit.

The discharge unit 15 is provided with a transport device 19 and a waste liquid tank 27 , and the liquid detected by the detection unit is discharged from the discharge port 26 to the waste liquid tank 27 by the transport device 19 .

Initially, in the sample unit 12, after a certain amount of oil 22 accommodated in the oil tank is supplied from the oil gauge tube 17 to the sample inlet 23, a certain amount of sample 21 is sucked into the sample gauge tube 16, The sample 21 is dispensed by sending a certain amount of the inhaled sample 21 into the liquid transport device 10 from the sample introduction port 23 . In this way, the dispensed certain amount of oil and the dispensed certain amount of sample are correspondingly arranged in the liquid delivery device 10 . At this time, since the sample is introduced after the oil is supplied to the sample introduction port 23, the sample can be introduced without adhering to the surface of the liquid transport device. On the other hand, also in the reagent unit 13 , after a certain amount of oil 22 is supplied from the oil probe 17 to the reagent inlet 24 , a certain amount of the reagent 25 is delivered from the reagent dispenser 18 and the reagent 25 is dispensed. In this way, a certain amount of oil to be dispensed and a certain amount of reagent to be dispensed are correspondingly arranged in the liquid delivery device 10 . In addition, like the sample, since the reagent is introduced after the oil is supplied to the reagent introduction port 24, the sample can be introduced without adhering to the surface of the liquid transport device. The sample 21 and reagent 25 dispensed in the liquid delivery device 10 are transported separately inside the liquid delivery device 10, detected by the detection unit 14, sucked from the discharge port 26 by the delivery device 19 of the discharge unit 15, and discharged to In the waste liquid tank 27.

FIG. 17 shows an example of the flow of operations within the liquid delivery device 10 . In the liquid transport device 10, first, the sample 21 is dispensed from the sample introduction port 23, and the reagent 25 is dispensed from the reagent introduction port 24, and then the dispensed sample 21 and the dispensed reagent 25 are mixed to form a reaction solution. After 31, the absorbance of the reaction solution 31 is measured, and finally the reaction solution 31 is discharged from the discharge port.

FIG. 18 is a diagram showing the arrangement of various parts for performing various operations in the liquid transport device 10 . In the liquid transport device 10, corresponding to each operation in FIG. 17, there are a sample introduction part 108 for introducing the sample 21, and a reagent introduction part 109 for introducing the reagent 25, and the sample 21 and the reagent 25 are combined and stirred to form a reaction solution. The mixing part 29 of 31, the measuring part 30 for measuring the reaction solution 31, the discharge part 110 for discharging the reaction solution 31 and other parts, each part is arranged in the liquid delivery device 10 in the order of the operation flow in Fig. 17, and each part is used. The liquid delivery channel 28 is connected. A control electrode is arranged on at least one of each of the liquid transport channel 28, the sample introduction part 108, the reagent introduction part 109, the mixing part 29, the measurement part 30, and the discharge part 110. By controlling the voltage applied to the control electrode, the test can be controlled. Transport and analysis of liquids such as samples and reagents. The measurement unit 30 is provided on at least a part of the liquid transport channel. The sample 21 dispensed from the sample introduction part 108 is transported through the liquid transport channel 28, mixed with the reagent 25 similarly dispensed and transported from the reagent introduction part 109 in the mixing part 29, and becomes the reaction solution 31, which is transported by the liquid. The channel 28 conveys, and after the measurement part 30 measures the component, it is conveyed and discharged from the discharge part 110 . In this way, various operations can be easily performed on a large number of samples since the liquid introduction part to the discharge part is arranged in the order of operations in the liquid transport device.

Fig. 19 shows an external view of the liquid transport device used in this example. In this embodiment, spacers 32 with a height of 0.5 mm are sandwiched between two upper and lower substrates 8 and 9, and fixed at a certain interval. In the case of filling the internal liquid delivery device with oil, although it is necessary to seal the side surface so that the oil does not leak out, in this embodiment, since the liquid is transported by surrounding the liquid with air-separated oil droplets, it is not necessary to seal the side surface. It is made so that no oil leaks, and the installation of the liquid conveying device becomes easy.

FIG. 20 shows the structure of the voltage control means 101 for operating the liquid 1 in the liquid delivery device 10. As shown in FIG. This control device is provided in the analysis system shown in FIG. 16 and has a control computer 102 and a communication unit 103 for applying a voltage controlled by the control computer 102 to predetermined electrodes in the liquid transport device 10 . A CRT, a printer, and a power supply are connected to the computer 102 for control. The computer 102 for control is provided with: an input unit for inputting appropriate conditions related to an analysis object and a liquid delivery method; a voltage control pattern storage unit for storing voltage control patterns corresponding to various liquid delivery methods; a voltage control pattern adjustment unit for determining a combination of voltage control patterns corresponding to the analysis object; and a voltage application control unit for applying a voltage corresponding to the combination of voltage control patterns determined by the voltage control pattern adjustment unit to the liquid transport device 10 . The communication part 103 is connected to the control electrode 5, and when the liquid 1 is controlled, the voltage controlled by the voltage application control part is applied to a predetermined electrode through the communication part 103 according to the information input from the input part.

FIG. 21 shows a cross-sectional configuration diagram of the sample introduction unit 108 . On the sample introduction port 23 of the upper side substrate 9, an oil gauge tube 17 for introducing the oil 22 contained in the container and a tube for dispensing the sample 21 contained in the container are respectively provided in a manner capable of moving up and down. Sample measuring tube 16. The reagent introduction part 109 can also have the same structure as that of FIG. 21 except that the sample tube 16 is replaced with the reagent tube 18 for dispensing the reagent 25 contained in the container. When the sample measuring tube 16 comes into contact with the oil droplet 2 dispensed into the sample introduction part 108 , the sample is dispensed separately into the oil droplet 2 . Therefore, it is possible to reliably place the sample sample in the oil droplet.

Furthermore, a hydrophobic film or the like may be applied to at least a part of the outer side of the sample tube 16 to impart lipophilicity. At this time, the affinity between the oil 22 and the sample tube 16 is improved, and the sample 21 can be sent out into the oil droplet 2 more reliably.

Here, although the structure in which one sample 21 is sent out as one droplet to the inside of one oil droplet 2 is shown, by applying a voltage to the control electrode 5 to move the droplet after sending one droplet, the One or more liquid droplets of the sample 21 are sent to the inside of the oil droplet 2 . In this case, liquid dispensing can be performed without affecting the liquid provided in the delivery device other than the oil droplet 2 to be delivered. Therefore, it is also possible to generate a situation where many liquids exist in one oil droplet as shown in FIG. 8 .

In addition, it is also possible to make the following structure, that is, as shown in FIG. 22, prepare the container 22' containing the oil 22 and the container 21' containing the sample 21, first, use the measuring tube 16 as shown in FIG. 22(a). After the oil 22 is sucked from the container 22', the sample 21 is sucked from the container 21' as shown in FIG. , and then each oil 22 is sent out and injected into the liquid delivery device 10 as shown in Fig. 22(c). In this way, as a structure for dispensing into the liquid delivery device, it is not necessary to provide two types of measuring tubes, the sample measuring tube and the oil measuring tube, and the structure can be simplified. In the structure of the present invention, by sending the sample 21 into the oil drop 2, when the test tube passes through the gas-liquid interface of the oil drop 2 and the air 3, it is subjected to the force caused by the surface tension of the gas-liquid interface. The sample 21 does not adhere to the measuring tube 16, and the minute sample 21 can be detached and dispensed stably. In addition, the reagent 25 can also be dispensed in the reagent introduction part 109 by the same method. Conventionally, in the liquid delivery device 10 filled with oil 22, when such a dispensing was performed, although the oil flow caused by the up and down of the oil interface and the introduced liquid occurred, by using the separated oil as in this embodiment The drop surrounds the liquid so that it can be dispensed without affecting other liquids within the liquid delivery device.

In addition, in this embodiment, the oil 22 is supplied to the reagent introduction part 108 using the oil probe 17, but the sample 21 surrounded by the oil 22 may be dispensed into the liquid delivery device 10 using a flow cell. Fig. 23 shows a cross-sectional structure of a reagent introduction part when a flow cell is used. The reagent 25 can also be dispensed in the reagent introduction part 109 in the same way. The sample tube 16 and the oil tube 17 are connected to the flow cell 33 , and the flow cell tube 34 extends from the flow cell 33 to the liquid delivery device 10 . After starting to send the quantity of sample 21 into the flow cell, the quantity of sample 21 is separated by causing the oil 22 to flow around the sample measuring tube 16, by squeezing each oil 22 into the liquid delivery device 10, Thus, the sample 21 surrounded by the oil droplets 2 is dispensed. With such a structure, the liquid can be dispensed into the liquid transport device 10 while the sample is in contact with the oil.

Alternatively, the oil 22 may be supplied by the valve 112 as shown in FIG. 29 . In this case, the oil suction time can be shortened, and when the sample is continuously dispensed, the oil supply time is shortened, and the efficiency can be improved. When supplying oil, connect the B side and the C side of the valve, and use the syringe 111 to store oil in the syringe. Next, the A side and the B side of the valve are communicated as shown in FIG. 29( a ), and the sample 21 is aspirated with the syringe 111 . The sample 21 can be dispensed by introducing the sample 21 into the liquid delivery device 10 with the syringe 111 as shown in FIG. 29(b). That is, the liquid delivery unit having a valve and a syringe sucks the sample after sucking the oil, sends out the sample surrounded by the oil, and introduces the sample surrounded by the oil into the liquid transfer device 10 . The reagent 25 can also be dispensed in the reagent introduction part 109 in the same way.

In the mixing section 29 , the combination and stirring of the sample 21 and the reagent 25 are performed by the method shown in Example 2. According to this embodiment, the flow of oil generated by mixing a plurality of liquids is not transmitted to other liquids other than the mixed liquid, and a plurality of liquids can be stably and independently operated in the liquid delivery device.

FIG. 24 shows configurations of the detection unit 14 and the measurement unit 30 . In this example, LED35 was used to measure the absorbance. The light 36 emitted from the LED 35 is incident on the reaction liquid 31 by the irradiation lens 37 , and the transmitted light is detected by the light emitting diode 38 . Though the present embodiment uses LED35, also can use halogen lamp 39 as shown in Figure 25, irradiate the light 36 that introduces by illuminating fiber 40 to measurement part 30 with irradiating lens 37, condense the transmission light on condensing lens 41 with irradiating lens 37. On the condensing optical fiber 42, the light is split into necessary wavelengths by a detection system 43 having a spectroscopic means for detection. In addition, in this embodiment, although light is used for measurement in the measurement part, it is also possible to use electrodes to measure the impedance of liquid or oil droplets, or arrange electrodes with ion-sensitive membranes to electrically measure the components inside the liquid.

In the case of filling the liquid delivery device with oil, when air bubbles enter from the inlet and outlet, although the possibility that the air bubbles will contact the liquid and affect the delivery and measurement of the liquid is considered, in order to remove the air bubbles from the oil, Since the air bubbles are brought into contact with the interface of oil and air, it is conceivable to remove the air bubbles by removing all the oil from the liquid delivery device and adding oil again. By surrounding the liquid with oil droplets as in the present embodiment, the air bubbles can be easily brought into contact with the interface between the oil and air, and the air bubbles can be removed from the oil. Therefore, stable measurement can also be performed during the measurement.

FIG. 26 shows a cross-sectional configuration diagram of the discharge unit 110 . The reaction liquid 31 and oil droplets 2 sent to the discharge port 26 are sucked by the transfer device 19 of the unit 15 and discharged to the waste liquid tank 27 . In the case where the inside of the liquid delivery device is filled with oil, when the liquid is discharged, a flow is generated in the oil by suction of the liquid and oil, but as in this embodiment, by discharging the liquid surrounded by oil droplets, there will be no flow caused by the oil Affects other liquids within the liquid delivery device, enabling stable manipulation of many liquids. Since the oil droplets 2 are discharged in the waste liquid tank 27, and the accumulated oil 22 and the reaction liquid 21 are separated by using the difference in specific gravity, even if many reaction liquids and oil droplets surrounding the reaction liquid are discharged, the treatment of the oil and the reaction liquid is difficult. easy. In addition, with conventional oil-filled liquid delivery devices, the oil in contact with the sample and reagent is subsequently contacted with the circulating sample and reagent, and the components of the sample and reagent are mixed through the oil. On the other hand, if this structure is used, since the sample, reagent, or reaction liquid and the oil in contact with it can be discharged together, the following sample, reagent, and components will not be mixed, and the oil in the liquid delivery device can be kept clean. .

Discharge Even if the discharge port and the conveying device 19 are not provided, discharge can be made from the side or bottom of the device. Fig. 27 shows an example thereof. An inclined surface is provided on the lower substrate 8, and the liquid is transported to the outlet with the inclined surface to be discharged, and then discharged to the waste liquid tank arranged at the lower part while being assisted by gravity. At this time, it is also possible to connect the waste liquid tank to the ground and discharge it to the waste liquid tank side by electric field force. In the conventional case where the inside of the liquid conveying device is filled with oil, it is considered that it is difficult to discharge the liquid 1 by sealing the side surface so as not to leak oil, but if this structure is used, it is not necessary to seal the side surface or the bottom surface It is made oil-tight, so liquid can be discharged from the side or bottom, and the knot of the discharge part becomes easy.

According to the structure of this embodiment, by enclosing the sample and the reagent in oil droplets and transporting them separately, it is possible to stably handle many liquids in the liquid transport device. In addition, although the application to the analysis system is aimed at the present embodiment, it is of course also applicable to the use of many liquids in the liquid transport device by adding the reaction liquid and the oil discharged from the discharge port to their respective containers. In the reaction system formed by the reaction, and in the mixing system producing a mixture of many liquids.

Example 6

This example illustrates a method of connecting multiple fluid delivery devices. By using a liquid delivery device with an unsealed side, multiple liquid delivery devices can be combined into one liquid delivery device. Figure 28 shows an example of combining two liquid delivery devices. The sides of the liquid delivery device are open to the outside, and a plurality of liquid delivery devices 10a and 10b are connected by an adhesive 44 on the sides of the liquid delivery device. A silicon-based adhesive is used as the adhesive 44 , but a Teflon (registered trademark)-based hydrophobic film or the like may be used to bring the liquid transport devices into contact with each other. In the case of using a Teflon (registered trademark)-based hydrophobic film, since adhesion is unnecessary, the liquid transport device can be easily separated after being connected. Also, it is configured such that the delivery channels in each fluid delivery device function as one fluid delivery channel when the devices are connected. The sample is introduced into the liquid transport device 10a from the sample introduction port 23, mixed with the reagent introduced from the reagent introduction port 24a at the mixing section 29a, transported through the liquid transport channel 28, and moved in the liquid transport device 10b. The reagent introduced from the reagent introduction port 24 b is mixed with the mixing unit 29 b in the liquid transport device 10 b and discharged from the discharge port 26 . In this way, a liquid can be transferred between a plurality of liquid transfer devices, and when reacting with a plurality of reagents and performing various operations, by connecting the liquid transfer devices, a plurality of operating parts can be arranged. In addition, when operating many liquids independently, although it is also possible to have a plurality of liquid delivery channels in one liquid delivery device, it is also possible to use a liquid delivery device to distribute many liquids to multiple liquid delivery devices. operate in. Previously, it was difficult to connect liquid delivery devices to each other because the sides were sealed inside the liquid delivery device filled with oil, but with the present invention, it is easy to connect multiple Liquid delivery device. As in this embodiment, if a plurality of liquid transport devices can be connected, the liquid transport device can be applied to analysis systems, reaction systems, and mixing systems that independently perform various operations on many liquids.

Example 7

FIG. 30 shows that the oil introduction port 113 is respectively provided at a place different from the sample introduction port 23 and the reagent introduction port 24, and is introduced into the liquid transport device 10 from the respective ports, and the sample is transported in the liquid transport device 10. 21 of the liquid and make it in contact with the oil analysis system. According to this configuration, liquid surrounded by oil is easily generated.

Fig. 31 shows a cross-sectional view of the sample introduction unit. The sample 21 is aspirated by the sample measuring tube 16 and dispensed from the sample introduction port 23 . The oil 22 is sucked with the oil measuring tube 17, and injected from the oil inlet 113. The sample 21 and the oil 22 introduced separately are positioned on the electrodes where the electrodes are arranged in the liquid transport device 10 . The sample 21 and oil 22 on the electrodes are transported at least in the order shown in Embodiment 4, that is, in the order shown in Figure 14 and Figure 15, of any one of the liquid droplet of the sample and the oil droplet, and can generate a sample surrounded by oil. liquid (droplet). For example, the sample 21 that is dividedly dispensed is delivered to the oil introduction part from the sample introduction part along the liquid delivery channel in which the control electrode is arranged, and at least a part of the oil 22 that is divided into the oil introduction part is enclosed as an oil The enclosed liquid surrounded by the droplet can then be transported along the liquid transport channel.

The structure of the mixing part and the discharge part can be made the same as that of the sixth embodiment.

Claims (13)

1. An analysis system having: a first unit having means for supplying the first liquid and means for supplying the second liquid; It is equipped with a first introduction part for introducing the first liquid from a member that supplies the first liquid, a discharge part for discharging the first liquid, a liquid delivery channel that has a plurality of electrodes and connects the introduction part and the discharge part, and is provided a second unit of a voltage applying member for applying a voltage to at least a part of the plurality of electrodes; A third unit equipped with a detection system for detecting the above-mentioned measurement unit; A fourth unit for discharging liquid from the above-mentioned discharge portion; characterized in that, The second liquid is immiscible with the first liquid, the first unit divides the second liquid and supplies it to the second unit so that the divided first liquid is contained therein, and the voltage applying member is applied to the plurality of A voltage is applied to at least a part of the electrodes so that a composite of the divided first liquid and the divided second liquid enclosing the divided first liquid moves along the liquid transport channel. 2. The analysis system according to claim 1, wherein: The means for supplying the first liquid has a container for containing the first liquid and a first measuring tube for sucking and sending out the first liquid, and when the first measuring tube is brought into contact with the second liquid introduced into the introduction part, the The above-mentioned first liquid is introduced into the inside of the above-mentioned introduced second liquid. 3. The analysis system according to claim 2, wherein: The means for supplying the above-mentioned second liquid has a second measuring tube for sucking and sending out the above-mentioned second liquid. 4. The analysis system according to claim 2, wherein: The above-mentioned first measuring tube is provided with a hydrophobic film on at least a part of its outer surface. 5. The analysis system according to claim 1, wherein: There is also a dispensing measuring tube for dispensing the above-mentioned first liquid and the above-mentioned second liquid, the part for supplying the above-mentioned first liquid has a first container for containing the above-mentioned first liquid, and the part for supplying the above-mentioned second liquid has a a second container containing the second liquid, the dispensing tube sucks the first liquid after sucking the second liquid from the second container, and introduces the sucked second liquid and the first liquid into the Second unit. 6. The analysis system according to claim 1, wherein: There is also a flow cell connected to the above-mentioned liquid delivery channel and a flow cell measuring tube connected to the above-mentioned flow cell. The part for supplying the above-mentioned first liquid has a first container for containing the above-mentioned first liquid and suction and delivery of the above-mentioned first liquid. A first measuring tube for a liquid, the part for supplying the second liquid has a second container for holding the second liquid and a second measuring tube for sucking and sending out the second liquid, and the measuring tube for the flow cell is fed from the above-mentioned The first tube and the second tube respectively introduce the first liquid and the second liquid, and the complex is introduced into the second unit by the flow of the second liquid. 7. The analysis system according to claim 1, wherein: The part for supplying the above-mentioned first liquid has a first container for containing the above-mentioned first liquid, and the part for supplying the above-mentioned second liquid has a second container for containing the above-mentioned second liquid and a liquid delivery device for sending the above-mentioned second liquid. The above-mentioned liquid delivery unit further has a suction unit, and the above-mentioned liquid delivery unit sucks the first liquid after sucking the second liquid from the second container to form the complex, and introduces the complex into the second container. unit. 8. The analysis system according to claim 1, wherein: The voltage applying member is a member for applying the voltage to move the plurality of complexes, and the surroundings of each of the plurality of complexes are filled with gas. 9. An analysis system having: a first unit having means for supplying the first liquid and means for supplying the second liquid; A first introduction part for introducing the first liquid from a member supplying the first liquid, a second introduction part for introducing the second liquid from a part supplying the second liquid, and a discharge part for discharging the first liquid, A liquid transport channel that has a plurality of electrodes and connects the first introduction part and the discharge part, a measurement part provided on at least a part of the liquid transport channel, and a voltage applying member that applies a voltage to at least a part of the plurality of electrodes. Dyad; A third unit comprising a detection system disposed adjacent to the measurement unit; A fourth unit for discharging liquid from the above-mentioned discharge portion; characterized in that, The above-mentioned second liquid is immiscible with the above-mentioned first liquid, the part for supplying the above-mentioned first liquid sends out the above-mentioned first liquid separately, and the part for supplying the above-mentioned second liquid sends out the above-mentioned second liquid separately, and the above-mentioned voltage applying part is applied to the above-mentioned A voltage is applied to at least a part of the plurality of electrodes so that the first liquid divided by the member for supplying the first liquid moves from the first introduction part to the second introduction part along the liquid transfer channel, and the first liquid introduced into the first liquid The first liquid contained in at least a part of the second liquid in the two introduction parts moves along the liquid delivery channel. 10. The analysis system according to claim 9, wherein: The first unit has a plurality of means for supplying the first liquid and a plurality of the first introduction parts. 11. The analysis system according to claim 9, wherein: The means for supplying the first liquid has a first measuring tube for sucking and delivering the first liquid, and the means for supplying the second liquid has a second measuring tube for sucking and delivering the second liquid. 12. The analysis system according to claim 11, wherein: The first measuring tube sends out the first liquid to the first introduction part, and the second measuring tube sends out the second liquid to the second introducing part. 13. The analysis system according to claim 9, wherein: The voltage applying means applies the voltage so that the first liquid contained in at least a part of the second liquid introduced into the plurality of second introduction parts is transferred, and the second liquid introduced into the plurality of second introduction parts Each of at least a part of the first liquid contained therein is filled with gas around the second liquid in which the first liquid is contained.

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