CN1858231A - Microbial sensitive diaphragm - Google Patents
Microbial sensitive diaphragm Download PDFInfo
- Publication number
- CN1858231A CN1858231A CN 200610016683 CN200610016683A CN1858231A CN 1858231 A CN1858231 A CN 1858231A CN 200610016683 CN200610016683 CN 200610016683 CN 200610016683 A CN200610016683 A CN 200610016683A CN 1858231 A CN1858231 A CN 1858231A
- Authority
- CN
- China
- Prior art keywords
- diaphragm
- microbial
- microorganism
- sensitive diaphragm
- avidin
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
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- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The present invention relates to a kind of microbial sensing diaphragm for microbial detection, and the microbial sensing diaphragm is used for detecting microbe in biological microscope. The microbial sensing diaphragm is one transparent film coated with avidin. The microbial sensing diaphragm is used as sensing and displaying element in microscopic examination with biological microscope, and the avidin has sensing and displaying effect on various kinds of microbes to result n fast and accurate detection of various kinds of microbes in biological microscope.
Description
Technical field
The present invention relates to a kind of microbial sensitive diaphragm that is used for microorganism detection.Can examine and determine multiple microorganism by the biomicroscope Direct Recognition by this microbial sensitive diaphragm.
Background technology
The accurate detection of microorganism is the distinct issues that biological educational circles, the world of medicine, food circle need need to be resolved hurrily.Generally adopt the culture dish cultured method to come culturing micro-organisms at present, observe the colony number in the culture dish afterwards, in order to judge multiple microorganism.This method exists shortcomings such as length detection time.
Biomicroscope microscopy microorganism is the biology method in common.Can see various microorganisms under the biomicroscope, but because the microorganism of being seen under the biomicroscope is very little, again owing to when biomicroscope microscopy microorganism, can not differentiate microorganism and impurity, therefore, can not use the method for biomicroscope microscopy when detecting various microorganism, need other proprietary microorganism detection methods.
And at present all there is complex operation in the detection method of the various microorganisms except that biomicroscope microscopy method, wastes time and energy or detects the lower shortcoming of accuracy rate.
Summary of the invention
The objective of the invention is to propose a kind of microbial sensitive diaphragm that is used for microorganism detection, can be implemented under the biomicroscope whether have microorganism in the Direct Recognition calibrating analyte by this microbial sensitive diaphragm.
A kind of microbial sensitive diaphragm that is used for microorganism detection of the present invention is to be coated with avidin on the diaphragm of a printing opacity.
Described printing opacity diaphragm is to be made by glass or plastics or the scraps of paper, and described avidin is coated on this diaphragm-operated surface by transparent adhesive tape.
Adopt microbial sensitive diaphragm of the present invention on biomicroscope, to detect method of microorganism and principle as follows:
1, is ready to the sample of material that contains microorganism that will detect;
2, the sample of material that will contain microorganism as requested is diluted to the aqueous solution of normality, carries out quantitative sampling then from this aqueous solution;
3, the above-mentioned sample of taking is applied on the microbial sensitive diaphragm of the present invention, certain time treats that the microorganism in the sample fully combines with avidin on the diaphragm;
4, place aseptic aqueous solution or other solution to clean the above-mentioned microbial sensitive diaphragm that had been coated with sample again, microorganism will be retained on this microbial sensitive diaphragm, other non-microorganism material then is dissolved in aseptic aqueous solution or other solution, this microbial sensitive diaphragm is taken out from sterilized water liquid or other solution, wait to do;
5, above-mentioned dried microbial sensitive diaphragm is placed direct viewing on the biomicroscope, on it as see and leave dirt settling and promptly illustrate in this detected sample and contain microorganism.
Sensing, the display element of the special use that microbial sensitive diaphragm of the present invention uses during as the biomicroscope microscopy, because sensing, demonstration take place when running into microorganism the avidin on the diaphragm, therefore can use the method for biomicroscope microscopy that microorganism is carried out accurate detection.
Embodiment
Specific descriptions by the following examples are described in further detail the present invention.
Embodiment 1
As diaphragm, cohere the microbial sensitive diaphragm of the present invention of avidin by the transparent glass thin slice on the surface.
Glass flake like the microbe carrier glass sheet that the use microscope is used or cover glass or the other types applies non-setting adhesive or other glue classes thereon, and avidin is cohered on coated glue, has made microbiological sensor diaphragm of the present invention.
Embodiment 2
As egative film, cohere the microbial sensitive diaphragm of the present invention of avidin by transparent plastic sheet on the surface.
Use sheet or web-like or other curved surface plastic tabs, apply non-setting adhesive or other glue classes thereon, then avidin is cohered on coated glue, made microbial sensitive diaphragm of the present invention.
Embodiment 3
By the thin printing opacity scraps of paper is diaphragm, coheres the microbial sensitive diaphragm of the present invention of avidin on the surface.
Use the thin printing opacity scraps of paper, apply non-setting adhesive or other glue classes thereon, then avidin is cohered on coated glue, made microbial sensitive diaphragm of the present invention.
Claims (2)
1. a microbial sensitive diaphragm that is used for microorganism detection is characterized in that it being to be coated with avidin on the diaphragm of a printing opacity.
2. microbial sensitive diaphragm according to claim 1 is characterized in that described printing opacity diaphragm is to be made by glass or plastics or the scraps of paper, and described avidin is coated on this diaphragm-operated surface by transparent adhesive tape.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200610016683 CN1858231A (en) | 2006-03-20 | 2006-03-20 | Microbial sensitive diaphragm |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200610016683 CN1858231A (en) | 2006-03-20 | 2006-03-20 | Microbial sensitive diaphragm |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1858231A true CN1858231A (en) | 2006-11-08 |
Family
ID=37297189
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200610016683 Pending CN1858231A (en) | 2006-03-20 | 2006-03-20 | Microbial sensitive diaphragm |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1858231A (en) |
-
2006
- 2006-03-20 CN CN 200610016683 patent/CN1858231A/en active Pending
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |