CN1850249A - Composition with function of relieving cough and calming asthma and preparing method - Google Patents

Abstract

The invention discloses a composition with functions of relieving cough and relieving asthma. , Chuanbei, Baiqian, licorice, aster, Ophiopogon japonicus, Bletilla striata, Shegan, Zhebei and other medicinal materials are used as raw materials, and are prepared by treating them with different physical or chemical methods according to the different physical and chemical properties of the effective components of each herb. Effective formulation.

Description

A composition with antitussive and antiasthmatic effect and preparation method thereof

technical field

The invention relates to a composition with the functions of relieving cough and relieving asthma, in particular to a composition with functions of relieving cough and relieving asthma which is made from Chinese herbal medicine.

Background technique

Cough and asthma is one of the most common chronic diseases in the world. At present, there are nearly 200 million patients in the world. At present, the annual cost of asthma treatment in the United States has exceeded 6 billion US dollars. The prevalence rate in my country is close to 1%, and the number of patients is more than 10 million. . It is a disease that seriously endangers people's physical and mental health, weakens their ability to work, and reduces their quality of life, and it is difficult to be cured. Asthma has become a serious public nuisance problem. Therefore, actively preventing and treating asthma, reducing the morbidity and mortality of asthma, and effectively controlling and alleviating the symptoms of asthma attacks are common tasks faced by the medical community all over the world. In recent years, especially in the past 10 years, great progress has been made in the research on the pathogenesis of asthma and the prevention and treatment of asthma. In recent years, in-depth studies on the pathogenesis of asthma have shown that bronchial asthma is not a kind of "reversible bronchial obstruction", but an airway that involves the participation of various inflammatory cells such as eosinophils, mast cells and T cells. Chronic inflammation. The pathogenesis involves a variety of inflammatory cells, especially the role and status of peripheral blood eosinophils (EOS) and T lymphocytes. This airway inflammation makes the susceptible person have airway responsiveness to various provocative factors, and can cause airway narrowing, manifested as recurrent wheezing, dyspnea, chest tightness or coughing and other symptoms, often at night and ( or) Onset and exacerbation in the morning, often with extensive and variable reversible airflow limitation, most patients can be treated or relieved by themselves.

Doctor trained in Western medicine thinks that anti-infection treatment is the basic principle of asthma treatment. Treatment drugs mainly include the following:

1 Glucocorticoids Glucocorticoids are still the most effective anti-inflammatory drugs at present. The essence of asthma is chronic non-specific airway inflammation. Hormones have effective anti-inflammatory effects, which can inhibit delayed allergic reactions and reduce airway sensitivity , but the side effects of hormones are large. There are many reports that excessive dosage of inhaled glucocorticoids will still produce hormone-like side effects, and may cause drug dependence or drug resistance. Clinically, steroid-resistant asthma and steroid-dependent asthma have appeared .

2 Theophylline Theophylline drugs have been used in the treatment of bronchial asthma and chronic obstructive pulmonary disease for decades. Theophylline can inhibit phosphodiesterase and increase the levels of cyclic adenosine monophosphate and cyclic guanosine monophosphate , diastolic bronchial smooth muscle also has: anti-inflammatory effect, immunoregulatory effect, antagonizing adenosine receptor, inducing B cell apoptosis, increasing diaphragmatic muscle tension, reducing diaphragmatic muscle fatigue, etc. Small, easy to cause poisoning and other adverse reactions.

3. β ₂ -receptor agonists β ₂ -receptor agonists were once promoted as the first choice drug for the treatment of bronchial asthma, but some research results show that standard treatment with B2 receptor agonists alone can make asthma worse .

4 Anticholinergic M-receptor blockers have a long history in the treatment of bronchial asthma. They can inhibit the release of acetylcholine from the motor nerves of the vagus nerve in bronchial smooth muscle to relieve asthma. However, due to their poor selectivity to M receptors, they can cause dry mouth, Systemic side effects such as thick sputum and central nervous system excitement.

Chinese medicine preparations should play an important role in the treatment of bronchial asthma, and they have a broad world. Meanwhile, Chinese medicines also have antitussive function in the treatment of asthma.

According to the guiding principles of clinical research on new Chinese medicines for the treatment of asthma, bronchial asthma can be divided into: cold wheeze, heat wheeze, wind wheeze and deficiency wheeze. Chinese patent 02132645 discloses a traditional Chinese medicine composition for treating asthma and its preparation method. Comprehensive treatment effect, so as to achieve the purpose of treating asthma. However, in addition to cough-relieving, phlegm-relieving and asthma-relieving drugs, the medicinal materials used mainly include Codonopsis pilosula, American ginseng, gecko, astragalus, medlar and other tonic traditional Chinese medicines, which are used for asthma due to deficiency, while the present invention is mainly used for asthma due to heat. At present, there are Linggui Kechuanning Capsules, Kechuan Pills, Miracle Loquat Zhike Granules, Jizhi Syrup and other products on the market, which have the effect of relieving cough and relieving asthma, but the curative effect is mediocre. Therefore need on the market a kind of medicine that can effectively control and alleviate asthma attack symptom for relieving cough and relieving asthma.

Contents of the invention

The object of the present invention is to provide a composition with antitussive and antiasthmatic effects, which can effectively control and alleviate the symptoms of asthma attacks.

Another object of the present invention is to provide a method for the preparation of the composition.

In order to achieve the above object, according to the understanding and treatment principles of cough and asthma diseases in our motherland, we refer to modern pharmacological research achievements and previous various proven prescriptions, remove the bad and extract the essence, and combine the new medicine of the present invention according to the theory of traditional Chinese medicine. square.

The raw material composition of preparation medicine of the present invention comprises as follows by weight ratio:

Coltsfoot 3-15 parts Bellflower 4-15 parts Lily 4-20 parts Mulberry 2-9 parts Chuanbei 2-9 parts Baiqian 2-9 parts

1-6 parts of Peucedanum, 1-6 parts of Chenpi, 5-20 parts of Trichosanthes, 3-15 parts of Licorice, 3-15 parts of Aster, 3-15 parts of Ophiopogon japonicus

Schisandra 1-6 parts Baibu 1-6 parts Almond 2-9 parts Zhebei 3-15 parts Shegan 1-6 parts Bletilla striata 2-9 parts

Agrimony 1-6 parts, mulberry leaves 2-9 parts, yam 10-50 parts.

The raw material for preparing the medicine of the present invention also contains the following components in weight ratio:

2-9 parts of green fruit 1-6 parts of mint 5-30 parts of ephedra 2-9 parts of burdock

15-60 parts of gypsum, 5-20 parts of jujube kernel, 3-15 parts of sand ginseng, 4-20 parts of donkey-hide gelatin.

The raw material for preparing the medicine of the present invention also contains the following components in weight ratio:

4-20 parts of ginger 8-40 parts of radish 40-200 parts of pear paste

10-60 parts of rock sugar, 5-25 parts of honey, 10-50 parts of lotus root.

The optimum weight proportion of above-mentioned raw material is:

Coltsfoot 6 parts Bellflower 7.5 parts Lily 9 parts Sangpi 4.5 parts Chuanbei 4.5 parts Peucedanum 3 parts

3 parts of tangerine peel 4.5 parts of Baiqian 10.5 parts of Gualou 6 parts of licorice 6 parts of aster 6 parts of Ophiopogon japonicus

Schisandra 3 parts Baibu 3 parts Almond 4.5 parts Zhebei 7.5 parts Shegan 3 parts Bletilla striata 4.5 parts

Agrimony 3 parts mulberry leaves 4.5 parts yam 24 parts green fruit 4.5 parts mint 3 parts ephedra 15 parts

30 parts of gypsum 9 parts of donkey-hide gelatin 4.5 parts of burdock seed 9 parts of jujube kernel 7.5 parts of sand ginseng 30 parts of rock sugar

8.9 parts of ginger, 20.75 parts of radish, 90 parts of pear paste, 12.5 parts of honey, 23.5 parts of lotus root.

Lily in the prescription mentioned above is Sunburned Lily, mulberry is Sunburned Mulberry, Ziyuan is Sunburned Ziyuan, Baibu is Sunburned Baibu; Almond is fried almond, Zaoren is fried Zaoren; Yam, ginger, radish, lotus root For raw or fresh products.

According to the conventional preparation process, the above raw materials can be prepared into common clinical pharmaceutical dosage forms, for example, pills, tablets, granules, oral liquids, capsules, ointments, emulsions, chewing agents, mixtures and the like.

Pills, oral liquids, mixtures and granules are preferred.

The preparation technology of preparation of the present invention is as follows:

Add 5-10 times water to the rest of the medicines except ginger, radish, lotus root, donkey-hide gelatin, honey, pear paste, and rock sugar, soak for 0.5-3 hours, add ginger, radish, and lotus root to decoct 1-4 times, add water 5-5 times each time 10 times water, decoct for 1-3 hours; combine the decoction, filter, concentrate under reduced pressure to contain 1-3g of medicinal materials per milliliter, add ethanol to 40%-85% alcohol content, cool down for 12-48 hours, filter After that, it is concentrated under reduced pressure to become an extract or dried, and then added donkey-hide gelatin powder, honey, pear extract, rock sugar and auxiliary materials in appropriate amounts to make various clinically acceptable dosage forms by conventional preparation methods, to obtain final product.

The composition of the invention has good curative effect in treating acute and chronic bronchial asthma. In order to show the therapeutic effect of the medicine of the present invention on acute and chronic bronchial asthma, we have done a large number of experimental studies, and the following experimental examples are used to further illustrate the present invention.

Pharmacodynamics of the preparation of the present invention

1. Antitussive effect

(1) Effect on cough caused by solid ammonia water in mice

Objective: To observe the effect of the present invention on the incubation period and frequency of coughing caused by ammonia water in mice.

Test drug

1. Drug name: The preparation of the present invention is produced by Lunan Pharmaceutical Co., Ltd.

2. Control drug: aminophylline tablets produced by Jilin Changbaishan Pharmaceutical Group Co., Ltd.

3. Reagent: Ammonia water, produced by Shanghai Reagent No. 1 Comprehensive Operation Company.

4. Instrument: Toshiba air compressor, made in Japan.

【animal】

1. Source, species, product name, qualification certificate: Provided by the Experimental Animal Center of Anhui Institute of Medical Sciences, Kunming mice, outbred closed strain, Wanyi Dongzhun No. 01.

2. Weight: 20±2g.

3. Gender: ♂♀ half and half.

4. Number of animals: 50 animals, 10 animals in each group, more than 50 animals are used for screening when purchased.

5. Experimental environment: the laboratory temperature is 18-28°C, and the relative humidity is 40-70%.

6. Pellet feed: provided by Fuyang Yingquan Kangle Experimental Animal Feed Co., Ltd.

【experiment method】

With reference to the method in the literature, 50 mice with a body weight of 18-22g were selected, half and half for ♂♀, and randomly divided into 5 groups, which were respectively normal saline group, control drug group, and small, medium and large doses of the preparation of the present invention. group (respectively equivalent to 5.0, 10, and 20 times of clinical use), were given 0.2ml/10g of the corresponding test solution by intragastric administration, once in the morning and afternoon of the first day, and 0.5h after the medicine was finished on the second day. The mice were placed in a 5000ml glass bell jar, and ammonia water (28%) was sprayed into the bell jar at a constant pressure of 400-500mmHg for 5 seconds, and then the cough latency and cough times (3min) of the mice were observed and recorded. Animals were screened 24 hours before the first administration, and then grouped after screening. Coughing criteria were: contraction of abdominal muscles, mouth wide open at the same time, sometimes with a coughing sound. If the incubation period is less than 1 minute, and the typical cough occurs more than 3 times within 1 minute, it is a sensitive animal with "cough", otherwise it will be eliminated as no cough.

【test results】

After small, medium and large doses of the present invention (respectively equivalent to 5.0, 10, 20 times of clinical use) are given to mice by intragastric administration for 3 times, the same as the dosage form of the control group, the cough incubation period of mice caused by ammonia water can be prolonged, and at the same time Compared with the normal saline group, the number of coughs was significantly reduced. Compared with the control group, the incubation period was P<0.05, P<0.05 and P<0.01, respectively, and the number of coughs was P<0.01 and P<0.01 compared with the control group. and P<0.01, but no significant dose-effect relationship. Prompt that the present invention has certain antitussive effect. see table 1

Table 1 preparation of the present invention is to the influence (X ± SD) of cough caused by solid ammonia water in mice group Number of animals (only) Incubation period (s) Number of coughs (times) Physiological saline control group The small dose of the present invention The medium dose of the present invention The large dose of the present invention 1010101010 41.3±10.958.5±15.7*55.8±16.4*57.6±18.5*59.3±12.1** 19.6±4.511.3±4.8**12.7±5.4**10.9±6.9**10.4±5.5**

Note: Compared with normal saline group, *P<0.05, **P<0.01

(2) Effect on guinea pigs cough caused by solid citric acid

【Test purpose】Observe the effect of the preparation of the present invention on the incubation period and frequency of cough caused by citric acid in guinea pigs.

【Test drug】

1. Drug name: The preparation of the present invention is produced by Lunan Pharmaceutical Co., Ltd.

2. Control drug: Aminophylline Tablets produced by Jilin Changbaishan Pharmaceutical Group Co., Ltd.

3. Reagent: citric acid, produced by China National Pharmaceutical Group Shanghai Chemical Reagent Company.

4. Instrument: Toshiba air compressor, made in Japan.

【animal】

1. Source, species, product name, certificate of conformity: British guinea pigs, provided by the Experimental Animal Center of Anhui Medical University.

2. Weight: 150-200g.

3. Gender: ♂♀ both

4. Number of animals: 40 animals, 8 animals in each group, more than 40 animals are used for screening when purchased.

5. Experimental environment: the laboratory temperature is 18-28°C, and the relative humidity is 40-70%.

6. Pellet feed: provided by Fuyang Yingquan Kangle Experimental Animal Feed Co., Ltd.

【experiment method】

With reference to the literature, take pure white guinea pigs with a body weight of 150-200 (sensitive to cough and asthma), and use them both for ♂♀. After screening 40 guinea pigs, they are randomly divided into 5 groups, each with 8 guinea pigs, respectively normal saline group (1.0ml/100g ), the contrast drug group, the small, medium and large dose groups of the preparation of the present invention are respectively equivalent to 1.875, 3.75, 7.5 times of the clinical dosage, and the administration volume is 1.0ml/100g. The experiment was carried out 0.5h after administration in the morning of the second day. Put the animal in a 5000ml airtight bell jar, spray 17.5% citric acid into the bell jar through the nozzle at a constant pressure of 400-500mmHg, spray for 1min, then observe and record the cough latency and cough frequency of the guinea pig within 6min, and compare between groups And make statistical analysis. Firstly, the guinea pigs were screened one day before the administration, and the guinea pigs who coughed less than 10 times or more than 30 times within 6 minutes were eliminated.

【test results】

The small, medium, and large doses of the present invention are equivalent to 1.875, 3.75, and 7.5 times of the clinical dosage. After intragastric administration to guinea pigs for 3 times, it can prolong the cough incubation period of guinea pigs caused by citric acid and reduce the number of coughs at the same time as the control group. , compared with the normal saline group, it has significant significance. Compared with the control group, the incubation period is P<0.05, P<0.05 and P<0.01 respectively, and the cough frequency is respectively P<0.05, P>0.05 and P<0.05 compared with the normal saline group. <0.05, suggesting that the present invention has a certain antitussive effect. see table 2

Table 2 small the present invention is to guinea pig because of the influence (X ± SD) of cough caused by citron group Number of animals (only) Incubation period (s) Number of coughs (times) Physiological saline control group The small dose of the present invention The medium dose of the present invention The large dose of the present invention 88888 61.5±29.1103.4±36.7*105.8±40.3*106.4±43.6*127.6±45.3** 16.5±6.210.0±4.7*10.5±4.5*11.0±4.910.5±3.7*

Note: Compared with normal saline group, *P<0.05, **P<0.01

2. expectorant effect

(1) Effects on the amount of phenol red sputum discharge in the respiratory tract of mice

[Test purpose] To observe the effect of the preparation of the present invention on the amount of sputum excretion in the respiratory tract of mice.

【Test drug】

1. Drug name: The preparation of the present invention is produced by Lunan Pharmaceutical Co., Ltd.

2. Control drug: Jizhi Syrup produced by Fuling Pharmaceutical Factory of Taiji Group

【Reagents and Instruments】

1. Phenol red: Xudong Chemical Factory, Chaoyang District, Beijing, batch number: 930609.

2. 721 Spectrophotometer, produced by Shanghai Third Analytical Instrument Factory.

3. 80-2 Sedimentation Centrifuge, product of Shanghai Surgical Instrument Factory.

4. A number of surgical instruments.

【animal】

1. Source, species, product name, qualification certificate: Provided by the Experimental Animal Center of Anhui Institute of Medical Sciences, Kunming mice, outbred closed strain, Wanyi Dongzhun No. 01.

2. Weight: 20±2g.

3. Gender: ♂♀ half and half.

4. Number of animals: 50, 10 in each group.

5. Experimental environment: the laboratory temperature is 18-28°C, and the relative humidity is 40-70%.

6. Pellet feed: provided by Fuyang Yingquan Kangle Experimental Animal Feed Co., Ltd.

【experiment method】

1. Determination of the maximum absorption peak of phenol red

Referring to the literature method, the determination of phenol red content was slightly changed. Precisely prepare phenol red mother solution with a concentration of 1mg/ml in a 100ml volumetric flask, and then prepare solutions containing 0.01, 0.1, 0.5, 0.7, 1, 3, 5, 10μg of phenol red per ml, and distribute them in eight 10ml volumetric flasks . Take two tubes of the solution randomly, and use the 0 tube of unknown phenol red as a control, and use a 721 spectrophotometer to measure the optical density within the wavelength range of 420-570nm. Finally, the wavelength of the maximum absorption peak of phenol red is at 560nm.

2. Draw a phenol red standard curve

Measure the optical density value of the phenol red solution prepared above at a wavelength of 560nm. As a result, the linear correlation between the content of phenol red in each tube and the optical density is very significant (correlation coefficient r=0.9990, P<0.001), each data And the linear equation is as follows:

Table 3 Phenol red standard curve and equation serial number Concentration (μg/ml) OD value Linear Equations and Correlation Coefficients 123456789 00.010.10.50.713510 00.0010.0060.0220.0320.0540.1680.3010.572 y=17.247x+0.0414 μg/ml R ² =0.9990 R=0.9995

3. Get 50 mice with a body weight of 18-22g, half and half for ♂♀, and be divided into 5 groups at random, which are respectively normal saline group, matched group (shortly supported syrup), small, middle and large dose groups of the preparation group of the present invention (respectively) Equivalent to 5, 10, and 20 times of the clinical dosage) were given 0.2ml/10g of the corresponding test solution by intragastric administration, once in the morning and afternoon of the first day, and tested 0.5h after the drug was given on the second day. Before the experiment, the mice were fasted for 8-12 hours, and each mouse was intraperitoneally injected with 0.5ml of phenol red solution after 30 minutes of administration. After 30 minutes, they were killed by devertebral dislocation and fixed in the supine position. The neck was straightened, the trachea was dissected, and a thread was threaded under the trachea for fixed endotracheal intubation. Endotracheal intubation (with a No. 9 needle ground flat on the head) is inserted into the trachea from the thyroid cartilage for about 0.3cm, ligated and fixed with the prepared silk thread, and 0.5ml of 5d/dl NaHCO ₃ solution is drawn into the trachea with a 1ml syringe Internally, push and pump repeatedly for 3 times, and then gavage the respiratory tract. Finally, use a syringe to extract the lavage fluid into the test tube, operate 3 times according to the above method, wash 9 times, absorb a total of 1.5ml of sodium carbonate solution, combine about 1.2-1.5ml of the eluate, and take the supernatant after centrifugation. The colorimeter was used, and the OD value was substituted into the equation to obtain the concentration of phenol red, and the results were analyzed statistically.

【test results】

After the small, medium and large doses of the present invention (respectively equivalent to 5, 10 and 20 times of the clinical dosage) are administered to mice for 3 times, the amount of phenol red expectoration in the respiratory tract of the mice can be increased, and compared with the normal saline group, it has Significant significance, respectively P<0.05, P<0.01 and P<0.05, compared with the control group, has a better pharmacological effect. see table 4

Table 4 The present invention is to the influence (X ± SD) of mouse respiratory tract phenol red sputum output group Number of animals (only) Respiratory tract phenol red concentration (μg/ml) Physiological saline control group The small dose of the present invention The medium dose of the present invention The large dose of the present invention 998109 2.728±0.9773.976±1.433*4.351±1.593*4.841±1.804**4.399±1.725*

Note: Compared with normal saline group, *P<0.05, **P<0.01

(2), the impact on rat capillary sputum output

【Test purpose】Observe the influence of the present invention on rat capillary sputum discharge.

【Test drug】

1. Drug name: the preparation of the present invention, produced by Lunan Pharmaceutical Co., Ltd.

2. Control drug: Linggui Kechuanning Capsule produced by Jiyuan General Pharmaceutical Factory.

【animal】

1. Source, species, product name, qualification certificate: Provided by the Experimental Animal Center of Anhui Medical University, Wister rats, outbred closed strain, Wanyi Dongzhun No. 02.

2. Weight: 200±20g.

3. Gender: ♂♀ half and half.

4. Number of animals: 30, 6 in each group.

5. Experimental environment: the laboratory temperature is 18-28°C, and the relative humidity is 40-70%.

6. Pellet feed: provided by Fuyang Yingquan Kangle Experimental Animal Feed Co., Ltd.

【Reagents and Instruments】

1. Capillary: inner diameter 0.8mm, length 5-6cm.

2. A number of surgical instruments.

3. Urethane: China National Pharmaceutical (Group) Shanghai Chemical Reagent Company, 20011105.

【experiment method】

Get body weight 180-220g rat 30, ♂♀ half and half, every group 6, be divided into 5 groups at random, be respectively normal saline group, matched group is given Linggui Kechuanning, preparation of the present invention is small, middle and large dosage group ( Respectively equivalent to 2.5, 5, and 10 times of the clinical dosage) were given 1.0ml/100g corresponding test solution by intragastric administration respectively. After fasting without food and water for 8-12 hours before the experiment, the rats were anesthetized by intraperitoneal injection of urethane 1.0 g/kg, and fixed in supine position (keep warm). Cut the skin in the middle of the neck and separate the trachea. Prick a small hole with a sharp injection needle between the cartilage rings in the middle of the lower edge of the thyroid cartilage, and then insert a glass capillary so that the capillary just touches the bottom surface of the trachea to suck the sputum from the rear of the trachea. When the sputum in the capillary is full, change another one immediately. The expectorant effect of drugs was evaluated by the length of sputum sucked by capillary. After recording the normal secretion 2 hours before the administration, continue to observe the sputum secretion for another 4 hours after intragastric administration (after 1 hour of administration, repeat the administration once at the same dose). The average hourly secretion and the sputum secretion compared with the normal saline group were compared before and after administration, and statistical analysis was made.

【test results】

After small, medium and large doses of the preparation of the present invention (equivalent to 2.5, 5, and 10 times of clinical dosage) are given to rats by intragastric administration for 2 times, the amount of expectoration in rat capillaries can be increased, which has significant significance compared with that of the normal saline group, respectively. It is P<0.05, P<0.05 and P<0.01; compared with before administration, it has very significant significance, respectively P<0.01, P<0.05 and P<0.001, compared with Linggui Kechuanning, it has better pharmacological effects. see table 5

Table 5 The present invention is to the influence (X ± SD) of rat capillary sputum output group Number of animals (only) Capillary sputum output (cm/h) Before administration after administration Physiological saline control group The small dose of the present invention The medium dose of the present invention The large dose of the present invention 66666 1.08±0.161.13±0.161.12±0.161.10±0.151.07±0.23 1.22±0.23 1.70±0.24* ^ΔΔΔ 1.72±0.38* ^ΔΔ 1.79±0.25** ^ΔΔΔ 1.77±0.27** ^ΔΔ

Note: Compared with normal saline group, *P<0.05, **P<0.01

3. Antiasthmatic effect——the effect on guinea pig asthma induced by histamine

[Test purpose] To observe the effect of the present invention on guinea pig asthma caused by histamine.

【Test drug】

1. Drug name: The preparation of the present invention is produced by Lunan Pharmaceutical Co., Ltd.

2. Control drug: Miracle Loquat Zhike Granule, Guizhou Miracle Pharmaceutical Co., Ltd.

3. Reagent: histamine phosphate, produced by China National Institute for the Control of Pharmaceutical and Biological Products, 25mg/bottle, batch number: 5109411.

4. Instrument: Toshiba air compressor, made in Japan.

【animal】

1. Source, species, product name, certificate of conformity: British guinea pigs, provided by the Experimental Animal Center of Anhui Medical University.

2. Weight 150-200g.

3. Gender: ♂♀ both.

4. Number of animals: 40 animals, 8 animals in each group, more than 40 animals are used for screening when purchased.

5. Experimental environment: the laboratory temperature is 18-28°C, and the relative humidity is 40-70%.

6. Pellet feed: provided by Fuyang Yingquan Kangle Experimental Animal Feed Co., Ltd.

【experiment method】

Get pre-selected 40 guinea pigs with a body weight of 150-200g, ♂♀ for both use, and randomly divided into 5 groups, 8 in each group, respectively normal saline group (1.0ml/100g), the control group was given Miracle Loquat Zhike Granules, the small, Medium and high dose groups (equivalent to 1.875, 3.75, 7.5 times of clinical dosage). The administration volume was 1.0ml/100g, and the administration was administered once in the morning and afternoon of the first day, and the experiment was carried out 0.5h after the administration in the morning of the second day. Put the animal in a 5000ml airtight bell jar, spray 1ml of 2mg/ml histamine phosphate with a pressure of 400-500mmHg, the animal will produce an "asthma" reaction after a certain incubation period after inhaling the above liquid, and the "asthma" reaction is as follows: The procedure was divided into 4 grades: Grade I rapid breathing, Grade II dyspnea, Grade III convulsions, and Grade IV falls. Once the animal convulsed, the cover was opened, and if necessary, artificial respiration was provided to prevent the animal from suffocating to death. Most animals can have III or IV grade reaction within 90S, generally not exceeding 150S, those exceeding 150S can be considered as insensitive, and will not be used. Screening is carried out the day before administration, and the incubation period exceeding 360S is calculated as 360S.

【test results】

After guinea pigs inhale a certain amount of histamine phosphate through the respiratory tract, they can create an "asthma" model similar to humans. After small, medium and large doses (equivalent to 1.875, 3.75 and 7.5 times of the clinical dosage) of the preparation of the present invention are administered to guinea pigs for 3 times, they can resist the "asthma" symptoms caused by histamine in animals, which is manifested as prolongation of the animal's fall latency, Compared with the normal saline group, it has very significant significance, after statistical processing, they are respectively P<0.01, P<0.001 and P<0.001. Compared with Miracle Loquat Zhike Granules, it has better pharmacological effects. See Table 6

Table 6 The influence (X ± SD) of preparation of the present invention on guinea pig asthma caused by histamine group Number of animals (only) Incubation period (s) Physiological saline control group The small dose of the present invention The medium dose of the present invention The large dose of the present invention 88888 110.6±38.5206.1±108.8*207.6±95.0*234.3±99.7**242.0±110.8**

Note: Compared with normal saline group, *P<0.05, **P<0.01

The following examples are for further disclosing the present invention, and it should be noted that these examples are only preferred modes of the present invention, and do not limit the scope of protection claimed by the present invention.

Example 1

Coltsfoot 3 parts Bellflower 15 parts Lily 20 parts Mulberry 9 parts Chuanbei 9 parts Baiqian 9 parts Agrimony 6 parts

6 parts of Peucedanum, 6 parts of tangerine peel, 20 parts of melon, 15 parts of licorice, 15 parts of aster, 15 parts of Ophiopogon japonicus, 9 parts of mulberry leaves

6 parts of Schisandra, 6 parts of Baibu, 9 parts of almond, 15 parts of Zhebei, 6 parts of Shegan, 9 parts of Bletilla striata, 50 parts of yam

Add 5 times of water to the above medicines, soak for 0.5 hours, decoct for 1 hour, filter, add 5 times of water, and decoct for 1 hour; combine the decoction liquid, filter, and concentrate under reduced pressure until the medicinal materials: water is about 1:1 , adjust the alcohol to 40%, cool down for 48 hours, filter, concentrate under reduced pressure to form an extract, add dextrin and powdered sugar to make granules, dry, make granules, and pack to obtain granules.

Example 2

Coltsfoot 15 parts Bellflower 4 parts Lily 20 parts Mulberry 9 parts Chuanbei 9 parts Baiqian 9 parts Agrimony 6 parts

Peucedanum 6 parts tangerine peel 6 parts melon 20 parts licorice 15 parts asters 15 parts radix 15 parts mulberry leaves 9 parts

6 parts of Schisandra, 6 parts of Baibu, 9 parts of almond, 15 parts of Zhebei, 6 parts of Shegan, 9 parts of Bletilla striata, 50 parts of yam

Add 10 times water to the above drug, soak for 3 hours, and decoct for 1 hour; combine the decoction liquid, filter, concentrate under reduced pressure until the medicinal material: water is about 2:1, adjust the alcohol to 85%, and refrigerate for 12 hours. Filtrate, concentrate under reduced pressure to form an extract and dry, add appropriate amount of dextrin and powdered sugar to make soft materials, granulate with a granulator, dry, and pack to obtain granules.

Example 3

Coltsfoot 15 parts Bellflower 15 parts Lily 4 parts Mulberry 9 parts Chuanbei 9 parts Baiqian 9 parts Agrimony 6 parts

6 parts of Peucedanum, 6 parts of tangerine peel, 20 parts of melon, 15 parts of licorice, 15 parts of aster, 15 parts of Ophiopogon japonicus, 9 parts of mulberry leaves

6 parts of Schisandra, 6 parts of Baibu, 9 parts of Almond, 15 parts of Zhebei, 6 parts of Shegan, 9 parts of Bletilla striata, 50 parts of Chinese yam

Add 8 times of water to the above medicine, soak for 1 hour, decoct for 3 hours, filter, add 10 times of water to the dregs of the medicine, and decoct for 2 hours; combine the decoction, filter, and concentrate under reduced pressure to contain 3g of medicinal materials per ml , add ethanol to 50% alcohol content, cool down for 48 hours, filter, concentrate under reduced pressure to form an extract, dry, add donkey-hide gelatin powder and appropriate amount of starch to make granules, fill capsules, and obtain capsules.

Example 4

Coltsfoot 15 parts Bellflower 15 parts Lily 20 parts Mulberry 2 parts Chuanbei 9 parts Baiqian 9 parts Agrimony 6 parts

6 parts of Peucedanum, 6 parts of tangerine peel, 20 parts of melon, 15 parts of licorice, 15 parts of aster, 15 parts of Ophiopogon japonicus, 9 parts of mulberry leaves

6 parts of Schisandra, 6 parts of Baibu, 9 parts of almond, 15 parts of Zhebei, 6 parts of Shegan, 9 parts of Bletilla striata, 50 parts of yam

Add the above medicines to 6 times of water, soak for 2 hours, decoct for 2 hours, filter, add 8 times of water to the medicinal residues, and decoct for 2 hours; combine the decoction liquid, filter, and concentrate under reduced pressure to contain 1g of medicinal materials per ml , add ethanol to 60% alcohol content, cool down for 48 hours, filter, concentrate under reduced pressure to obtain an extract, dry, add powdered sugar and starch in an appropriate amount to make granules, and press into tablets to obtain tablets.

Example 5

Coltsfoot 15 parts Bellflower 15 parts Lily 20 parts Mulberry 9 parts Chuanbei 2 parts Baiqian 9 parts Agrimony 6 parts

6 parts of Peucedanum, 6 parts of tangerine peel, 20 parts of melon, 15 parts of licorice, 15 parts of aster, 15 parts of Radix Ophiopogon japonicus, 9 parts of mulberry leaves

6 parts of Schisandra, 6 parts of Baibu, 9 parts of almond, 15 parts of Zhebei, 6 parts of Shegan, 9 parts of Bletilla striata, 50 parts of yam

Add 8 times of water to the above medicine, soak for 1 hour, decoct for 1.5 hours, filter, add 8 times of water to the medicinal residue, decoct for 2 hours, filter, add 8 times of water to the medicinal residue, decoct for 2 hours, filter filter, concentrate under reduced pressure to contain 1.5g of medicinal materials per milliliter, add ethanol to contain 70% alcohol, cool down for 24 hours, filter, concentrate under reduced pressure to obtain an extract, dry, add appropriate amount of starch, Pan made into pills, that is, pills.

Example 6

Coltsfoot 15 parts Bellflower 15 parts Lily 20 parts Mulberry 9 parts Chuanbei 9 parts Baiqian 2 parts Agrimony 6 parts

6 parts of Peucedanum, 6 parts of tangerine peel, 20 parts of melon, 15 parts of licorice, 15 parts of aster, 15 parts of Ophiopogon japonicus, 9 parts of mulberry leaves

6 parts of Schisandra, 6 parts of Baibu, 9 parts of Almond, 15 parts of Zhebei, 6 parts of Shegan, 9 parts of Bletilla striata, 50 parts of Chinese yam

Add 10 times of water to the above medicine, soak for 1.5 hours, decoct for 2 hours, filter, add 8 times of water to the medicinal residue, decoct for 1.5 hours, filter, add 8 times of water to the medicinal residue, decoct for 1 hour, filter filter, concentrate under reduced pressure to contain 2 g of medicinal materials per milliliter, add ethanol to contain 70% alcohol, cool down for 24 hours, filter, concentrate under reduced pressure to obtain an extract, dry, mix, and sieve , that is, powder.

Example 7

Coltsfoot 15 parts Bellflower 15 parts Lily 20 parts Mulberry 9 parts Chuanbei 9 parts Baiqian 9 parts Agrimony 6 parts

1 part Peucedanum, 6 parts tangerine peel, 20 parts melon, 15 parts licorice, 15 parts aster, 15 parts Ophiopogon japonicus, 9 parts mulberry leaves

6 parts of Schisandra, 6 parts of Baibu, 9 parts of Almond, 15 parts of Zhebei, 6 parts of Shegan, 9 parts of Bletilla striata, 50 parts of Chinese yam

The above medicines are crushed and sieved, an appropriate amount of adhesive honey is added, and pellets are obtained to obtain pills.

Example 8

Coltsfoot 15 parts Bellflower 15 parts Lily 20 parts Mulberry 9 parts Chuanbei 9 parts Baiqian 9 parts Agrimony 6 parts

6 parts of Peucedanum, 1 part of tangerine peel, 20 parts of melon, 15 parts of licorice, 15 parts of aster, 15 parts of Ophiopogon japonicus, 9 parts of mulberry leaves

6 parts of Schisandra, 6 parts of Baibu, 9 parts of Almond, 15 parts of Zhebei, 6 parts of Shegan, 9 parts of Bletilla striata, 50 parts of Chinese yam

Add 10 times of water to the above medicine, soak for 2 hours, decoct for 3 hours, filter, add 10 times of water to the medicinal residue, decoct for 2 hours, filter, add 8 times of water to the medicinal residue, decoct for 1.5 hours, filter Overdose, combine the decoction liquid, filter, concentrate under reduced pressure to 3g of medicinal materials per milliliter, add ethanol to contain 65% alcohol, cool down for 48 hours, filter, concentrate under reduced pressure until medicinal materials: water is about 2:1, adjust Alcohol reaches 80%, cool down for 48 hours, filter, concentrate under reduced pressure to form extract and alcohol reflux extract, add donkey-hide gelatin solution, add 10 times water, stir, cool down for 48 hours, filter, concentrate the filtrate, To adjust the pH to 4, add 0.1% activated carbon, heat and boil for 30 minutes, filter, add water for injection to the full amount, filter, fine filter, freeze-dry, aseptically subpackage, test, and obtain the powder injection.

Example 10

Coltsfoot 15 parts Bellflower 15 parts Lily 20 parts Mulberry 9 parts Chuanbei 9 parts Baiqian 9 parts Agrimony 6 parts

6 parts of Peucedanum, 6 parts of tangerine peel, 5 parts of Trichosanthes, 15 parts of licorice, 15 parts of aster, 15 parts of Ophiopogon japonicus, 9 parts of mulberry leaves

6 parts of Schisandra, 6 parts of Baibu, 9 parts of Almond, 15 parts of Zhebei, 6 parts of Shegan, 9 parts of Bletilla striata, 50 parts of Chinese yam

Add 8 times of water to the above medicine, soak for 1.5 hours, decoct for 1.5 hours, filter, add 8 times of water to the medicinal residue, decoct for 1.5 hours, filter, add 6 times of water to the medicinal residue, decoct for 1.5 hours, filter Over, combine the decoction liquid, filter, concentrate under reduced pressure to 2g of medicinal material per milliliter, add ethanol to 75% alcohol content, cool down for 48 hours, add 8 times of water, cool down for 48 hours, filter, and concentrate the filtrate to Adjust the pH to 7, add 0.1% activated carbon, heat and boil for 30 minutes, filter, add water for injection to the full amount, filter, fine filter, seal and sterilize, and obtain the infusion solution.

Example 11

Coltsfoot 15 parts Bellflower 15 parts Lily 20 parts Mulberry 9 parts Chuanbei 9 parts Baiqian 9 parts Agrimony 6 parts

6 parts of Peucedanum, 6 parts of tangerine peel, 20 parts of melon, 3 parts of licorice, 15 parts of aster, 15 parts of Ophiopogon japonicus, 9 parts of mulberry leaves

6 parts of Schisandra, 6 parts of Baibu, 9 parts of Almond, 15 parts of Zhebei, 6 parts of Shegan, 9 parts of Bletilla striata, 50 parts of Chinese yam

Add 5 times of water to the above medicine, soak for 1.5 hours, decoct for 3 hours, filter, add 10 times of water to the medicinal residue, decoct for 2 hours, filter, add 8 times of water to the medicinal residue, decoct for 1.5 hours, filter Add 8 times more water to the dregs of the medicine, decoct for 1.5 hours, filter, combine the decoction, concentrate under reduced pressure to contain 2.5g of medicinal materials per milliliter, add ethanol to 55% alcohol content, cool down for 48 hours, filter, Concentrate under reduced pressure into an extract, add ethanol of specified concentration, stir, and filter to obtain tincture.

Example 12

Coltsfoot 15 parts Bellflower 15 parts Lily 20 parts Mulberry 9 parts Chuanbei 9 parts Baiqian 9 parts Agrimony 6 parts

6 parts of Peucedanum, 6 parts of tangerine peel, 20 parts of melon, 15 parts of licorice, 3 parts of aster, 15 parts of Ophiopogon japonicus, 9 parts of mulberry leaves

6 parts of Schisandra, 6 parts of Baibu, 9 parts of almond, 15 parts of Zhebei, 6 parts of Shegan, 9 parts of Bletilla striata, 50 parts of yam

Add 10 times of water to the above medicine, soak for 0.5 hours, decoct for 3 hours, filter, add 10 times of water to the medicinal residue, decoct for 2 hours, filter, add 8 times of water to the medicinal residue, decoct for 1.5 hours, filter After passing, combine the decoction liquid, filter, concentrate under reduced pressure to 2g of medicinal materials per milliliter, add ethanol to contain 55% alcohol, cool down for 24 hours, filter, concentrate under reduced pressure to obtain extract, dry, heat and melt the matrix, add Dry the medicinal powder, mix it evenly, pour it into a suppository mold coated with a release agent, cool it down, and take it out to obtain a suppository.

Example 13

Coltsfoot 15 parts Bellflower 15 parts Lily 20 parts Mulberry 9 parts Chuanbei 9 parts Baiqian 9 parts Agrimony 6 parts

6 parts of Peucedanum, 6 parts of tangerine peel, 20 parts of melon, 15 parts of licorice, 15 parts of aster, 3 parts of Ophiopogon japonicus, 9 parts of mulberry leaves

6 parts of Schisandra, 6 parts of Baibu, 9 parts of Almond, 15 parts of Zhebei, 6 parts of Shegan, 9 parts of Bletilla striata, 50 parts of Chinese yam

Add 10 times of water to the above medicine, soak for 0.5 hours, decoct for 2.5 hours, filter, add 10 times of water to the medicinal residues, decoct for 2 hours, filter, add 8 times of water to the medicinal residues, decoct for 1.5 hours, filter Overdose, combine the decoction liquid, filter, concentrate under reduced pressure to 2g of medicinal material per milliliter, add ethanol to contain 60% alcohol, cool down for 24 hours, filter, concentrate under reduced pressure to obtain extract, add appropriate amount of water, filter, and Add an appropriate amount of sucrose and water, heat to boil, dissolve, filter, concentrate to make syrup, mix with the above concentrate, boil, let cool, add preservatives and flavors, add water to dilute to obtain syrup.

Example 14

Coltsfoot 15 parts Bellflower 15 parts Lily 20 parts Mulberry 9 parts Chuanbei 9 parts Baiqian 9 parts Agrimony 6 parts

6 parts of Peucedanum, 6 parts of tangerine peel, 20 parts of melon, 15 parts of licorice, 15 parts of aster, 15 parts of Ophiopogon japonicus, 9 parts of mulberry leaves

Schisandra 1 part Baibu 6 parts Almond 9 parts Zhebei 15 parts Shegan 6 parts Bletilla striata 9 parts yam 50 parts

Add 6 times of water to the above medicine, soak for 0.5 hours, decoct for 1 hour, filter, add 10 times of water to the medicinal residue, decoct for 1 hour, filter, add 8 times of water to the medicinal residue, decoct for 0.5 hour, filter Overdose, combine the decoction liquid, filter, concentrate under reduced pressure to contain 1.5g of medicinal materials per milliliter, add ethanol to contain 50% alcohol, cool down for 48 hours, filter, concentrate under reduced pressure to obtain extract, add appropriate amount of water, filter, Add water to the full amount, filter, fill, and sterilize to obtain the mixture.

Example 15

Coltsfoot 15 parts Bellflower 15 parts Lily 20 parts Mulberry 9 parts Chuanbei 9 parts Baiqian 9 parts Agrimony 6 parts

6 parts of Peucedanum, 6 parts of tangerine peel, 20 parts of melon, 15 parts of licorice, 15 parts of aster, 15 parts of Ophiopogon japonicus, 9 parts of mulberry leaves

6 parts of Schisandra, 1 part of Baibu, 9 parts of almond, 15 parts of Zhebei, 6 parts of Shegan, 9 parts of Bletilla striata, 50 parts of yam

Add 6 times of water to the above medicine, soak for 0.5 hours, decoct for 1 hour, filter, add 10 times of water to the medicinal residue, decoct for 1 hour, filter, add 8 times of water to the medicinal residue, decoct for 0.5 hour, filter Pass, combine the decoction liquid, filter, concentrate under reduced pressure to 1.5g of medicinal materials per milliliter, add ethanol to contain 50% alcohol, cool down for 48 hours, filter, concentrate under reduced pressure to obtain extract, dry and pulverize, add appropriate amount of paste Essence, magnesium stearate, stevioside, mixed evenly, and granulated by a dry granulator to obtain the product.

Example 16

Coltsfoot 15 parts Bellflower 15 parts Lily 20 parts Mulberry 9 parts Chuanbei 9 parts Baiqian 9 parts Agrimony 6 parts

6 parts of Peucedanum, 6 parts of tangerine peel, 20 parts of melon, 15 parts of licorice, 15 parts of aster, 15 parts of Ophiopogon japonicus, 9 parts of mulberry leaves

6 parts of Schisandra, 6 parts of Baibu, 2 parts of Almond, 15 parts of Zhebei, 6 parts of Shegan, 9 parts of Bletilla striata, 50 parts of Chinese yam

Add 6 times of water to the above medicine, soak for 0.5 hours, decoct for 1 hour, filter, add 10 times of water to the medicinal residue, decoct for 1 hour, filter, add 8 times of water to the medicinal residue, decoct for 0.5 hour, filter Overdose, combine the decoction liquid, filter, concentrate under reduced pressure to contain 1.5g of medicinal materials per milliliter, add ethanol to contain 50% alcohol, cool down for 48 hours, filter, concentrate under reduced pressure to obtain extract, add appropriate amount of water, filter, Take another amount of sucrose and water, heat to boil, dissolve, filter, concentrate to make syrup, mix with the above concentrate, boil, let cool, add preservatives and flavors, add water to dilute to obtain syrup.

Example 17

Coltsfoot 15 parts Bellflower 15 parts Lily 20 parts Mulberry 9 parts Chuanbei 9 parts Baiqian 9 parts Agrimony 6 parts

6 parts of Peucedanum, 6 parts of tangerine peel, 20 parts of melon, 15 parts of licorice, 15 parts of aster, 15 parts of Ophiopogon japonicus, 9 parts of mulberry leaves

6 parts of Schisandra, 6 parts of Baibu, 9 parts of Almond, 3 parts of Zhebei, 6 parts of Shegan, 9 parts of Bletilla striata, 50 parts of Chinese yam

Add the above medicine to 4 times of water, soak for 0.5 hours, add water to 10 times of water, decoct for 2 hours, filter, add 8 times of water to the medicinal residue, decoct for 1.5 hours, filter, combine the decoction liquid, filter, Concentrate under reduced pressure to contain 2 g of medicinal materials per milliliter, add ethanol to contain 50% alcohol, cool down for 12 hours, filter, concentrate under reduced pressure to obtain an extract, dry, add sugar powder, granulate, dry, and pack to obtain granules.

Example 18

Coltsfoot 15 parts Bellflower 15 parts Lily 20 parts Mulberry 9 parts Chuanbei 9 parts Baiqian 9 parts Agrimony 6 parts

6 parts of Peucedanum, 6 parts of tangerine peel, 20 parts of melon, 15 parts of licorice, 15 parts of aster, 15 parts of Ophiopogon japonicus, 9 parts of mulberry leaves

6 parts of Schisandra, 6 parts of Baibu, 9 parts of Almond, 15 parts of Zhebei, 1 part of Shegan, 9 parts of Bletilla striata, 50 parts of Chinese yam

Add 4 times of water to the above medicine, soak for 0.5 hours, add water to 8 times of water, decoct for 2.5 hours, filter, add 6 times of water to the medicine dregs, decoct for 2 hours, filter, combine the decoction liquid, filter, reduce Concentrate under pressure to contain 1g of medicinal materials per ml, add ethanol to contain 60% alcohol, cool down for 24 hours, filter, concentrate under reduced pressure to obtain an extract, dry, add lactose and aspartame, mix well, dry granulate, pack, That is, granules.

Example 19

Coltsfoot 15 parts Bellflower 15 parts Lily 20 parts Mulberry 9 parts Chuan Bei 9 parts Baiqian 9 parts Agrimony 1 part

6 parts of Peucedanum, 6 parts of tangerine peel, 20 parts of melon, 15 parts of licorice, 15 parts of aster, 15 parts of Ophiopogon japonicus, 9 parts of mulberry leaves

6 parts of Schisandra, 6 parts of Baibu, 9 parts of Almond, 15 parts of Zhebei, 6 parts of Shegan, 9 parts of Bletilla striata, 50 parts of Chinese yam

Add 5 times of water to the above medicine, soak for 0.5 hours, add water to 10 times of water, decoct for 3 hours, filter, add 8 times of water to the medicine dregs, decoct for 2 hours, filter, combine the decoction liquid, filter, reduce Concentrate under pressure to contain 3g of medicinal materials per milliliter, add ethanol to contain 45% alcohol, cool down for 24 hours, filter, concentrate under reduced pressure to obtain an extract, dry, add starch, mix evenly, dry granulate, pack into capsules, and obtain capsules .

Example 20

Coltsfoot 15 parts Bellflower 15 parts Lily 20 parts Mulberry 9 parts Chuanbei 9 parts Baiqian 9 parts Agrimony 6 parts

6 parts of Peucedanum, 6 parts of tangerine peel, 20 parts of melon, 15 parts of licorice, 15 parts of aster, 15 parts of Ophiopogon japonicus, 9 parts of mulberry leaves

6 parts of Schisandra, 6 parts of Baibu, 9 parts of almond, 15 parts of Zhebei, 6 parts of Shegan, 2 parts of Bletilla striata, 50 parts of yam

Add 5 times of water to the above medicine, soak for 0.5 hours, add water to 10 times of water, decoct for 3 hours, filter, add 8 times of water to the medicine dregs, decoct for 2 hours, filter, combine the decoction liquid, filter, reduce Concentrate under pressure to contain 3g of medicinal materials per ml, add ethanol to contain 45% alcohol, cool down for 24 hours, filter, concentrate under reduced pressure to obtain an extract, dry, add starch and mix, dry granulate, tablet, quality inspection, packaging , that is, tablets.

Example 21

Coltsfoot 15 parts Bellflower 15 parts Lily 20 parts Mulberry 9 parts Chuanbei 9 parts Baiqian 9 parts Agrimony 6 parts

6 parts of Peucedanum, 6 parts of tangerine peel, 20 parts of melon, 15 parts of licorice, 15 parts of aster, 15 parts of Ophiopogon japonicus, 2 parts of mulberry leaves

6 parts of Schisandra, 6 parts of Baibu, 9 parts of Almond, 15 parts of Zhebei, 6 parts of Shegan, 9 parts of Bletilla striata, 50 parts of Chinese yam

Add 6 times of water to the above medicine, soak for 1 hour, add water to 10 times of water, decoct for 2.5 hours, filter, add 8 times of water to the medicine dregs, decoct for 2 hours, filter, combine the decoction, filter, reduce Concentrate under pressure to contain 2.5g of medicinal materials per milliliter, add ethanol to 75% alcohol content, precipitate for 12 hours, filter, concentrate under reduced pressure to obtain an extract, add 10 times the amount of water, cool down for 12 hours, filter, concentrate, add water to the full amount , filtering, filling, sterilizing, quality inspection, and packaging to obtain the oral liquid.

Example 22

Coltsfoot 15 parts Bellflower 15 parts Lily 20 parts Mulberry 9 parts Chuanbei 9 parts Baiqian 9 parts Agrimony 6 parts

6 parts of Peucedanum, 6 parts of tangerine peel, 20 parts of melon, 15 parts of licorice, 15 parts of aster, 15 parts of Ophiopogon japonicus, 9 parts of mulberry leaves

6 parts of Schisandra, 6 parts of Baibu, 9 parts of almond, 15 parts of Zhebei, 6 parts of Shegan, 9 parts of Bletilla striata, 10 parts of yam

Add water to decoct the above medicine twice, add 10 times of water each time, and decoct for 2 hours; combine the decoction liquid, filter, concentrate under reduced pressure to contain 2 g of medicinal material per ml, add ethanol to contain 45% alcohol, and cool down for 12 hours, filter, concentrate under reduced pressure to extract 10 times the amount of water, cool down for 24 hours, filter, the filtrate is concentrated to 4 grams of raw medicinal materials per ml, add 6 times of water to cool down, filter, add water to the filtrate to the full amount, pour Pack, sterilize, and obtain the oral liquid.

Example 23

Coltsfoot 6 parts Bellflower 7.5 parts Lily 9 parts Mulberry 4.5 parts Chuanbei 4.5 parts Peucedanum 3 parts

3 parts of tangerine peel 4.5 parts of Baiqian 10.5 parts of Trichosanthes 6 parts of licorice 6 parts of asters 6 parts of Ophiopogon japonicus

Schisandra 3 parts Baibu 3 parts Almond 4.5 parts Zhebei 7.5 parts Shegan 3 parts Bletilla striata 4.5 parts

Agrimony 3 parts mulberry leaves 4.5 parts yam 24 parts green fruit 4.5 parts mint 3 parts ephedra 15 parts

30 parts of gypsum 9 parts of donkey-hide gelatin 4.5 parts of burdock seed 9 parts of jujube kernel 7.5 parts of sand ginseng 30 parts of rock sugar

8.9 parts of ginger, 20.75 parts of radish, 90 parts of pear paste, 12.5 parts of honey, 23.5 parts of lotus root.

Add water to decoct the above medicines for 2 times, add 8 times of water each time, and decoct for 1 hour each time; combine the decoction liquid, filter, concentrate under reduced pressure to contain 2 g of medicinal materials per ml, add ethanol to 50% alcohol content, cool Sink for 24 hours, filter, concentrate under reduced pressure to form an extract, add 10 times the amount of water, precipitate for 24 hours, filter, concentrate, heat and dissolve donkey-hide gelatin, honey, pear extract, and rock sugar, then combine with the medicinal solution, filter, and add water to The whole amount is filtered, filled and sterilized to obtain the oral liquid.

Example 24

Coltsfoot 6 parts Bellflower 7.5 parts Lily 10 parts Mulberry 4.5 parts Chuanbei 4.5 parts Baiqian 4.5 parts

3 parts of Peucedanum, 3 parts of tangerine peel, 10 parts of melon, 7.5 parts of licorice, 7.5 parts of aster, 7.5 parts of Ophiopogon japonicus

6 parts of Schisandra 6 parts of Baibu 4.5 parts of Almond 7.5 parts of Zhebei 3 parts of Shegan 4.5 parts of Bletilla striata

Agrimony 3 parts, mulberry leaves 4.5 parts, yam 25 parts. 4.5 parts of green fruit 3 parts of mint 15 parts of ephedra

4.5 parts of burdock seed, 30 parts of gypsum, 10 parts of jujube kernel, 10 parts of donkey-hide gelatin, 7.5 parts of sand ginseng

Add water to decoct the above medicines for 3 times, add 6 times of water each time, and decoct for 1.5 hours each time; combine the decoction liquid, filter, concentrate under reduced pressure to contain 1g of medicinal materials per ml, add ethanol until the alcohol content is 70%, and cool Settled for 48 hours, filtered, concentrated under reduced pressure to form extract, dried, crushed, pulverized donkey-hide gelatin, rock sugar and drug extract powder, mixed with honey and pear extract, added an appropriate amount of wetting agent to make granules, dried, packaged, that is Get granules.

Example 25

Coltsfoot 6 parts Bellflower 7.5 parts Lily 9 parts Mulberry 4.5 parts Chuanbei 4.5 parts Peucedanum 3 parts

3 parts of tangerine peel 4.5 parts of Baiqian 10.5 parts of Trichosanthes 6 parts of licorice 6 parts of asters 6 parts of Ophiopogon japonicus

3 parts of Schisandra 3 parts of Baibu 4.5 parts of Almond 7.5 parts of Zhebei 3 parts of Shegan 4.5 parts of Bletilla striata

Agrimony 3 parts mulberry leaves 4.5 parts yam 24 parts green fruit 4.5 parts mint 3 parts ephedra 15 parts

30 parts of gypsum 9 parts of donkey-hide gelatin 4.5 parts of burdock seed 9 parts of jujube kernel 7.5 parts of sand ginseng 30 parts of rock sugar

8.9 parts of ginger, 20.75 parts of radish, 90 parts of pear paste, 12.5 parts of honey, 23.5 parts of lotus root

Add 10 times water to other medicines except ginger, radish, lotus root, donkey-hide gelatin, honey, pear paste and rock sugar, soak for 1 hour, add ginger, radish and lotus root to decoct twice, add 8 times water each time, decoct each time 1 hour; combine the decoction, filter, concentrate under reduced pressure to contain 2g of medicinal materials per ml, add ethanol to 55% alcohol content, cool down for 24 hours, filter, concentrate under reduced pressure to obtain an extract, add 10 times the amount of water, and precipitate After 24 hours, filter, heat and dissolve donkey-hide gelatin, honey, pear extract, and rock sugar, combine with the liquid medicine, filter, add water to the full amount, filter, fill, and sterilize to obtain the oral liquid.

Claims (8)

1, a kind of compositions with function of relieving cough and calming asthma is characterized in that it is made by the raw material that comprises following weight parts:

Flos Farfarae 3-15 part Radix Platycodonis 4-15 part Bulbus Lilii 4-20 part Cortex Mori 2-9 part Bulbus Fritillariae Cirrhosae 2-9 part Rhizoma Cynanchi Stauntonii 2-9 part

Radix Peucedani 1-6 part Pericarpium Citri Reticulatae 1-6 part Fructus Trichosanthis 5-20 part Radix Glycyrrhizae 3-15 part Radix Asteris 3-15 part 3-15 Radix Ophiopogonis part

Fructus Schisandrae Chinensis 1-6 part Radix Stemonae 1-6 part Semen Armeniacae Amarum 2-9 part Bulbus Fritillariae Thunbergii 3-15 part Rhizoma Belamcandae 1-6 part Pseudobulbus Bletillae (Rhizoma Bletillae) 2-9 part

Herba Agrimoniae 1-6 part Folium Mori 2-9 part Rhizoma Dioscoreae 10-50 part.

2, compositions according to claim 1 is characterized in that it also comprises:

Fructus Canarii 2-9 part Herba Menthae 1-6 part Herba Ephedrae 5-30 part Fructus Arctii 2-9 part

Gypsum Fibrosum 15-60 part Semen Ziziphi Spinosae 5-20 part Radix Adenophorae (Radix Glehniae) 3-15 part Colla Corii Asini 4-20 part.

3, compositions according to claim 2 is characterized in that it also comprises:

Rhizoma Zingiberis Recens 4-20 part Radix Raphani 8-40 part pear paste 40-200 part

Crystal sugar 10-60 part Mel 5-25 part Rhizoma Nelumbinis 10-50 part.

4, compositions according to claim 3 is characterized in that said raw material weight ratio is:

3 parts of 4.5 parts of Radix Peucedanis of 4.5 parts of Bulbus Fritillariae Cirrhosae of 9 parts of Cortex Mori of 7.5 portions of Bulbus Liliies of 6 parts of Radix Platycodoniss of Flos Farfarae

6 parts of 6 parts of Radix Asteriss of 10.5 portions of Radix Glycyrrhizaes of 4.5 parts of Fructus Trichosanthis of 3 parts of Rhizoma Cynanchi Stauntoniis of Pericarpium Citri Reticulatae 6 parts of Radix Ophiopogonis

4.5 parts of 3 portions of Pseudobulbus Bletillae (Rhizoma Bletillae)s of 7.5 parts of Rhizoma Belamcandae of 4.5 parts of Bulbus Fritillariae Thunbergii of 3 parts of Semen Armeniacae Amarums of 3 parts of Radixs Stemonae of Fructus Schisandrae Chinensis

15 parts in 3 portions of Herba Ephedraes of 4.5 portions of Herba Menthaes of 24 portions of Fructus Canariis of 4.5 portions of Rhizoma Dioscoreaes of 3 parts of Folium Mori of Herba Agrimoniae

30 parts in 7.5 portions of crystal sugar of 9 parts of Radix Adenophorae (Radix Glehniae) of 4.5 parts of Semen Ziziphi Spinosaes of 9 parts of Fructus Arctiis of 30 parts of Colla Corii Asini of Gypsum Fibrosum

23.5 parts of 12.5 portions of Rhizoma Nelumbinis of 90 parts of Mel of 20.75 parts of pear pastes of 8.9 portions of Radix Raphani of Rhizoma Zingiberis Recens.

5, according to the described compositions of claim 1-4, it is characterized in that Bulbus Lilii is a honey-fried BULBUS LILII in the said composition, Cortex Mori is a baked mulberry bark, and Radix Asteris is for processing Radix Asteris, and the Radix Stemonae is a Radix Stemonae (processed); Semen Armeniacae Amarum is a stir-baked SEMEN ARMENIACAE AMARUM, and Semen Ziziphi Spinosae is a Semen Ziziphi Spinosae (parched); Rhizoma Dioscoreae, Rhizoma Zingiberis Recens, Radix Raphani, Rhizoma Nelumbinis make a living product or bright product.

6,, it is characterized in that the made dosage form of said composition is any receptible dosage form clinically according to the described compositions of claim 1-5.

7, compositions according to claim 6 is characterized in that the said dosage form of said composition is pill, oral liquid, mixture, granule.

8,, it is characterized in that it prepares according to following method according to the described compositions of claim 1-7:

All the other medicines except that Rhizoma Zingiberis Recens, Radix Raphani, Rhizoma Nelumbinis, Colla Corii Asini, Mel, pear paste, crystal sugar are added 5-10 times of water, soaked 0.5-3 hour, input Rhizoma Zingiberis Recens, Radix Raphani, Rhizoma Nelumbinis decoct 1-4 time, add water 5-10 times water at every turn, decoct 1-3 hour; Merge decoction liquor, filter, be evaporated to every milliliter and contain medical material 1-3g, add ethanol to containing pure 40%-85%, Cryoprecipitation 12-48 hour, filter, concentrating under reduced pressure becomes extractum or drying, add Colla Corii Asini powder, Mel, pear paste, crystal sugar and right amount of auxiliary materials and make clinical receptible various dosage forms, promptly with the conventional formulation method.