CN1736489A - Process for preparing nano-granule of albumin - Google Patents
Process for preparing nano-granule of albumin Download PDFInfo
- Publication number
- CN1736489A CN1736489A CNA2004100466775A CN200410046677A CN1736489A CN 1736489 A CN1736489 A CN 1736489A CN A2004100466775 A CNA2004100466775 A CN A2004100466775A CN 200410046677 A CN200410046677 A CN 200410046677A CN 1736489 A CN1736489 A CN 1736489A
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- Prior art keywords
- albumin
- solution
- present
- granule
- preparing nano
- Prior art date
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- 108010088751 Albumins Proteins 0.000 title claims abstract description 13
- 102000009027 Albumins Human genes 0.000 title claims abstract description 13
- 239000008187 granular material Substances 0.000 title abstract 2
- 238000004519 manufacturing process Methods 0.000 title abstract 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 10
- 108091003079 Bovine Serum Albumin Proteins 0.000 claims abstract description 4
- 229940098773 bovine serum albumin Drugs 0.000 claims abstract description 4
- 239000003431 cross linking reagent Substances 0.000 claims abstract description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 3
- 238000002360 preparation method Methods 0.000 claims description 6
- 238000013019 agitation Methods 0.000 claims description 3
- 230000001476 alcoholic effect Effects 0.000 claims description 3
- 230000006837 decompression Effects 0.000 claims description 3
- 239000003229 sclerosing agent Substances 0.000 claims description 3
- 239000012153 distilled water Substances 0.000 claims description 2
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 abstract description 2
- 238000005119 centrifugation Methods 0.000 abstract 1
- 239000003795 chemical substances by application Substances 0.000 abstract 1
- 229960000587 glutaral Drugs 0.000 abstract 1
- 238000003756 stirring Methods 0.000 abstract 1
- 239000003814 drug Substances 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 238000001415 gene therapy Methods 0.000 description 3
- 230000008685 targeting Effects 0.000 description 3
- 210000004185 liver Anatomy 0.000 description 2
- 230000000259 anti-tumor effect Effects 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 238000002512 chemotherapy Methods 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000002502 liposome Substances 0.000 description 1
- 201000007270 liver cancer Diseases 0.000 description 1
- 208000014018 liver neoplasm Diseases 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 239000002539 nanocarrier Substances 0.000 description 1
- 230000002980 postoperative effect Effects 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 239000013603 viral vector Substances 0.000 description 1
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- Medicinal Preparation (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
Disclosed is a process for preparing nano-granule of albumin which comprises, charging certain amount of bovine serum albumin into water, adjusting pH with HCl, slowly loading ethanol solution, instilling right amount of glutaric dialdehyde as hardening agent, stirring, stewing 2h at room temperature, decompressing to volatilize residual ethanol, subjecting the solution to centrifugation treatment, finally removing residual albumin and cross linking agent.
Description
Technical field
The present invention relates to a kind of preparation method to gene therapy of liver cancer medicine nano-carrier.
Technical background
Primary hepatocarcinoma is one of most popular 10 kinds of malignant tumor in the world, and annual about 38.6 ten thousand people in the whole world die from hepatocarcinoma, and wherein 45% in China.Present routine clinical treatment, the excision rate is low, and the chemotherapy targeting is poor, and toxic and side effects is obvious.In recent years, gene therapy obtains a lot of achievements aspect basic research, however because the defective of viral vector, and liposome easily degrades, factors such as instability make gene therapy be subjected to certain restriction.
Summary of the invention
Purpose of the present invention is that a kind of preparation method with albumin nano granular that targeting is strong, medicine comprises the rate height, drug release rate is controlled will be provided.
The preparation method of albumin nano granular of the present invention is: get a certain amount of bovine serum albumin and add in the entry, adjust the ph value with HCl, slowly pour alcoholic solution to solution into and milky occurs, splash into an amount of sclerosing agent glutaraldehyde, agitation as appropriate at room temperature leaves standstill 2h, after decompression makes remaining ethanol volatilization, gained solution is removed remaining albumin and cross-linking agent by centrifugal treating, promptly obtains albumin nano granular of the present invention.
The albumin nano granular that the present invention obtains has the liver property that significantly becomes to liver, and its Targeting Performance significantly reduces the toxicity of chemotherapeutics, can improve curative effect.Can also improve the antitumous effect of chemotherapeutics, significantly suppress the growth of minimal disease, reduce postoperative recurrence greatly.
Specific implementation method
Specifically introduce the step of a albumin nano granular of preparation below in conjunction with embodiment.
Getting an amount of bovine serum albumin adds in the 100ml distilled water, adjust ph value to 5.5 with 0.1N HCl, slowly pour alcoholic solution into, milky occurs until solution, dropwise splash into the sclerosing agent that concentration is 0.06～12.5 μ g/mg, agitation as appropriate, at room temperature leave standstill 2h, after decompression made remaining ethanol volatilization, gained solution centrifugal 30min when 17000rmp promptly got the product that the present invention obtains to remove remaining albumin and cross-linking agent.
Claims (1)
1, a kind of preparation method of albumin nano granular, making and settlement are equipped with the product that a the present invention obtains, the steps include: to get an amount of bovine serum albumin adds in the 100ml distilled water, adjust ph value to 5.5 with 0.1N HCl, slowly pour alcoholic solution into, milky appears until solution, dropwise splash into the sclerosing agent that concentration is 0.06～12.5 μ g/mg, agitation as appropriate, at room temperature leave standstill 2h, after decompression made remaining ethanol volatilization, gained solution centrifugal 30min when 17000rmp promptly got the product that the present invention obtains to remove remaining albumin and cross-linking agent.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2004100466775A CN1736489A (en) | 2004-08-19 | 2004-08-19 | Process for preparing nano-granule of albumin |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2004100466775A CN1736489A (en) | 2004-08-19 | 2004-08-19 | Process for preparing nano-granule of albumin |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1736489A true CN1736489A (en) | 2006-02-22 |
Family
ID=36079560
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNA2004100466775A Pending CN1736489A (en) | 2004-08-19 | 2004-08-19 | Process for preparing nano-granule of albumin |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1736489A (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103169968A (en) * | 2013-03-12 | 2013-06-26 | 中国科学院理化技术研究所 | Hydrophobic chlorin photosensitizer nano-drug preparation based on albumin, preparation method and application thereof |
| CN103356485A (en) * | 2012-04-10 | 2013-10-23 | 青岛大学医学院附属医院 | Hirudin albumin nanoparticles and preparation method thereof |
| WO2016000653A1 (en) * | 2014-07-03 | 2016-01-07 | Cspc Zhongqi Pharmaceutical Technology (Shijiazhuang) Co., Ltd. | Purified therapeutic nanoparticles and preparation methods thereof |
| CN108801736A (en) * | 2018-06-29 | 2018-11-13 | 闽南师范大学 | A kind of acetaldehyde crosslinking bovine serum albumin(BSA) and its preparation and application |
| CN109528627A (en) * | 2018-12-03 | 2019-03-29 | 深圳大学 | A kind of Ru-BSA hydrogel and the preparation method and application thereof |
| WO2020135123A1 (en) * | 2018-12-24 | 2020-07-02 | 华中科技大学 | Method for preparing albumin nanoparticles based on free radical oxidation |
-
2004
- 2004-08-19 CN CNA2004100466775A patent/CN1736489A/en active Pending
Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103356485A (en) * | 2012-04-10 | 2013-10-23 | 青岛大学医学院附属医院 | Hirudin albumin nanoparticles and preparation method thereof |
| CN103356485B (en) * | 2012-04-10 | 2019-06-07 | 青岛大学附属医院 | Hirudin albumin nano granular and preparation method thereof |
| CN103169968B (en) * | 2013-03-12 | 2014-11-26 | 中国科学院理化技术研究所 | Hydrophobic chlorin photosensitizer nano-drug preparation based on albumin, preparation method and application thereof |
| CN103169968A (en) * | 2013-03-12 | 2013-06-26 | 中国科学院理化技术研究所 | Hydrophobic chlorin photosensitizer nano-drug preparation based on albumin, preparation method and application thereof |
| CN106687110B (en) * | 2014-07-03 | 2020-09-18 | 石药集团中奇制药技术（石家庄）有限公司 | A kind of purified therapeutic nanoparticle and preparation method thereof |
| WO2016000653A1 (en) * | 2014-07-03 | 2016-01-07 | Cspc Zhongqi Pharmaceutical Technology (Shijiazhuang) Co., Ltd. | Purified therapeutic nanoparticles and preparation methods thereof |
| CN106687110A (en) * | 2014-07-03 | 2017-05-17 | 石药集团中奇制药技术（石家庄）有限公司 | A purified therapeutic nanoparticle and its preparation method |
| US10500165B2 (en) | 2014-07-03 | 2019-12-10 | Cspc Zhongqi Pharmaceutical Technology (Shijiazhuang) Co., Ltd. | Purified therapeutic nanoparticles and preparation methods thereof |
| CN108801736A (en) * | 2018-06-29 | 2018-11-13 | 闽南师范大学 | A kind of acetaldehyde crosslinking bovine serum albumin(BSA) and its preparation and application |
| CN108801736B (en) * | 2018-06-29 | 2021-07-30 | 闽南师范大学 | A kind of acetaldehyde cross-linked bovine serum albumin and its preparation and application |
| CN109528627A (en) * | 2018-12-03 | 2019-03-29 | 深圳大学 | A kind of Ru-BSA hydrogel and the preparation method and application thereof |
| CN109528627B (en) * | 2018-12-03 | 2022-04-15 | 深圳大学 | Ru-BSA hydrogel and preparation method and application thereof |
| WO2020135123A1 (en) * | 2018-12-24 | 2020-07-02 | 华中科技大学 | Method for preparing albumin nanoparticles based on free radical oxidation |
| US11318105B2 (en) | 2018-12-24 | 2022-05-03 | Huazhong University Of Science And Technology | Method for preparing albumin nanoparticles based on free radical oxidation |
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| C06 | Publication | ||
| PB01 | Publication | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |