CN1731152A - All-fiber completely solidified biological micro-array chip scanner - Google Patents
All-fiber completely solidified biological micro-array chip scanner Download PDFInfo
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- CN1731152A CN1731152A CN 200510028878 CN200510028878A CN1731152A CN 1731152 A CN1731152 A CN 1731152A CN 200510028878 CN200510028878 CN 200510028878 CN 200510028878 A CN200510028878 A CN 200510028878A CN 1731152 A CN1731152 A CN 1731152A
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Abstract
The invention relates to an all optical fiber all solid biology micro array chip scanner in the field of biological medical treatment detector technology. It comprises: a plurality of lasers, a wavelength-division multiplexer, a light circular, a light alidade, a biology chip, a plurality of electric filters, a plurality of light probes and a data read card, wherein the plurality of lasers are separately connected with the wavelength-division multiplexer by optical fiber; the wavelength-division multiplexer is connected with the light circular by optical fiber; one end of the light circular is connected with the light alidade by optical fiber; the biology chip is laid on one end of the light alidade; the other end of the light circular is series connected with the plurality of electric filters which are connected with the plurality of light probes; the plurality of light probes are connected with the data read card by data wire.
Description
Technical field
What the present invention relates to is a kind of scanner of technical field of measurement and test, specifically, is a kind of all-fiber completely solidified biological micro-array chip scanner.
Background technology
Biochip is a new technology that develops rapidly in the current life science; by the miniature organism chemical analysis system of micro-processing technology at the solid surface structure; can realize to protein, DNA and other biological component accurately, fast, the detection and the analysis of large information capacity, be the powerful measure of obtaining associated biomolecule information efficiently on a large scale.The nineties in last century, U.S. Affymatrix company realized that the high density of dna probe molecule is integrated, had released business-like genetic chip and chip scanning reading apparatus.Biochip scanner adopts laser confocal scanning instrument or CCD camera technique usually.The laser co-focusing chip scanner is made excitation source with laser, and photomultiplier detects, thereby sensitivity is higher, can detect every square micron several molecule at zero point fluorescence.Produce minimum hot spot behind the laser focusing, therefore have higher resolution, can reach several microns.Focus on the back in addition and produce the generation of strength laser fluorescence excitation, can improve detection sensitivity greatly.And utilize its high-velocity scanning, and action time is extremely short, thereby avoids photobleaching, and the laser co-focusing chip scanner is that present technology is the most advanced, the scanner that applicability is best.This class scanner typically has the ScanArray family chip scanner of AppliedBioScience company.
Find by prior art documents, the paper that S.W.Paddock delivered " Confocallaser scanning microscopy " (laser co-focusing microscan) .Biotechniques, 1999,27 (5): 992-996, (1999 be published in " biotechnology "), with Michael B.Sinclair, Jerilyn A.Timlin, David M.Haaland, the paper that and Margaret Werner-Washburne is delivered " Design; construction; characterization; and application of a hyperspectral microarrayscanner " (high its design of spectrum microarray scanner, structure, feature and application) APPLIED OPTICS_Vol.43, this two literary composition of No.10_1 April 2004 (2004 be published in " applied optics ") describes typical laser co-focusing and CCD chip scanner technology in detail, the confocal system of scanner is by the reflection/transmission diaphragm, prism, attenuator, gather the fluorescence object lens, survey object lens, narrow band filter slice, the aperture space becomes the phase element to form, laser enters light path system after reflection, attenuator decay laser energy, reflection enters object lens again, object lens focus the light beam on the chip, scattering fluorescence on the chip is received by object lens again simultaneously, after reflection by narrow band filter slice filtering, surveys object lens focusing, after the aperture space becomes to carry out filtering mutually, receive fluorescence information by photomultiplier.Its weak point is: for to make optical system stable operation and adjustment that enough body spaces and heavy base must be arranged, cause the bulky and preponderance of scanner 1.; If the increase of exciting light sources number is body volume and weight extremely difficult control in product design then.2. the scanner inner optical system is very responsive to vibrations, carrying or move the precision very easily cause optical system and lose, as survey enter behind the object lens focusing aperture space Cheng Xiangqi precision with regard to very easily because of vibrations are shifted, make noise improve.3. processing technology is complicated and with high costs, and adjustment needs professional optical engineering personnel.4. to the parasitic light sensitivity.
Summary of the invention
The object of the invention is to overcome deficiency of the prior art, a kind of all-fiber completely solidified biological micro-array chip scanner is provided, make it connect each specific optical device by optical fiber, reduce the susceptibility of each device to various vibrations and temperature, improve optical stability and instrument adaptability, solve Machine Design and processing problems that complicated and high optical system is adjusted effectively, thereby effectively reduced the cost of scanner.
The present invention is achieved by the following technical solutions, the present invention includes: several laser instruments, wavelength division multiplexer, optical circulator, optical collimator, biochip, several wave filters, several photo-detectors, the data read card, several laser instruments are connected with wavelength division multiplexer by optical fiber respectively, wavelength division multiplexer is connected by optical fiber with optical circulator, one end of optical circulator is connected by optical fiber with optical collimator, biochip is held level with both hands towards one of optical collimator emission light signal and is put, the other end of optical circulator is connected in series by optical fiber with several wave filters successively, several wave filters are connected by optical fiber with several photo-detectors respectively, several photo-detectors link to each other wave filter by data line with the data read card, the individual number average of photo-detector is identical with the number of laser instrument.
Described laser instrument, its wavelength excitation wavelength with its corresponding biochip coloring agent respectively are identical.
Described wavelength division multiplexer, its operation wavelength is identical with the wavelength of its corresponding laser instrument.
Described optical collimator can be the optical fiber GRIN Lens, also can be Liar.
Described wave filter, its operation wavelength is identical with its corresponding laser instrument institute excited fluorescent wavelength.
Described photo-detector is photomultiplier, avalanche diode, photodiode or semiconductor optical device.
The course of work of the present invention is: the LASER Light Source that several laser instruments produce is respectively after Optical Fiber Transmission, synthesize into optical fiber by the wavelength division multiplexer connection, light sources emission multi beam different wavelength of laser is to wavelength division multiplexer, after transmitting, simple optical fiber enters optical circulator by its synthetic single channel light, light is by behind the optical circulator, project after focusing on through optical collimator again and do the generation of dual wavelength fluorescence excitation on the biochip, the fluorescence excitation signal of being collected by collimating apparatus enters optical fiber by collimating apparatus, is resent in the optical circulator.The light of exporting from the another port of optical circulator enters several wave filters of wavelength of fluorescence correspondence with it again, leach the fluorescence excitation of different wave length respectively by several wave filters, survey by several photo-detectors respectively and change into electric signal, read by the data read card again.
The invention has the beneficial effects as follows: 1. because of adopting all solid stateization design, machinery and optical stability are fabulous, and all elements are almost identical with semiconductor element, long service life; 2. light path system design transmits light signal with optical fiber, and is more insensitive and can significantly save the whole machine body space to parasitic light, dwindles cabinet size, alleviates instruments weight, and the body mechanism design is more flexible; But 3., can develop more kinds ofly exciting light wavelength input synchronously and can not allow volume and weight out of control because of the mechanism design flexible operating, simultaneously also can the combination of relevant biochemistry detection equipment structure dress with other, raising product surcharge.4. simplify production procedure in a large number, make it to be easy to control, must not invest high optic test carrier and optical instrument on production line, reduce the production line cost of investment, thereby make cost decreases; 5. it is more insensitive to vibrations that all solid stateization optical element is located the back in body mechanism, is easy to carrying, for ease of maintenaince maintenance.6. all solid stateization optical element provides objective specification quantized data, and adjustment efficient height is more easily trained the maintenance personal.7. being the three-dimensional or two-dimentional free space transmission of optical signals is converted in the optical fiber of one dimension with other technologies scheme difference, light signal being transmitted be easier to control and management, is to innovate on the light path system structure with regard to biochip scanner.
Description of drawings
Fig. 1 is a structural representation of the present invention
Embodiment
As shown in Figure 1, the present invention includes: laser instrument 1,2, wavelength division multiplexer 3, optical circulator 4, optical collimator 5, biochip 6, wave filter 7, wave filter 8, photo-detector 9, photo-detector 10, data read card 11, laser instrument 1,2 are connected with wavelength division multiplexer 3 by two optical fiber respectively, wavelength division multiplexer 3 and optical circulator 4 are connected by optical fiber, one end of optical circulator 4 and optical collimator 5 are connected by optical fiber, biochip 6 is held level with both hands towards one of optical collimator 5 emission light signals and is put, the other end of optical circulator 4 successively with wave filter 7, wave filter 8 is connected in series by optical fiber, wave filter 7, wave filter 8 respectively with photo-detector 9, photo-detector 10 connects by optical fiber, photo-detector 9, photo-detector 10 links to each other with data read card 11 by data line.
Described two laser instruments 1,2, its wavelength excitation wavelength with its corresponding biochip coloring agent respectively are identical.
Described wavelength division multiplexer 3, its operation wavelength is identical with the wavelength of its corresponding laser instrument 1,2.
Described optical collimator 5 is optical fiber GRIN Lens or Liar.
Described wave filter 7, its operation wavelength are identical with 1 excited fluorescent wavelength of its corresponding laser instrument respectively.Described wave filter 8, its operation wavelength are identical with 2 excited fluorescent wavelength of its corresponding laser instrument respectively.1 excited fluorescent of laser instrument enters photo-detector by wave filter 7, and 2 excited fluorescent of laser instrument enter photo-detector by wave filter 8.
Described photo- detector 9,10 is photomultiplier, avalanche diode, photodiode or semiconductor optical device.
Claims (6)
1. all-fiber completely solidified biological micro-array chip scanner, comprise: several laser instruments (1,2), wavelength division multiplexer (3), optical circulator (4), optical collimator (5), biochip (6), several wave filters (7,8), several photo-detectors (9,10), data read card (11), it is characterized in that, several laser instruments (1,2) be connected with wavelength division multiplexer (3) by optical fiber respectively, wavelength division multiplexer (3) is connected by optical fiber with optical circulator (4), one end of optical circulator (4) is connected by optical fiber with optical collimator (5), biochip (6) is held level with both hands towards one of optical collimator (5) emission light signal and is put, the other end of optical circulator (4) successively with several wave filters (7,8) be connected in series by optical fiber, several wave filters (7,8) respectively with several photo-detectors (9,10) connect by optical fiber, several photo-detectors (9,10) link to each other wave filter (7 by data line with data read card (11), 8), photo-detector (9,10) individual number average and laser instrument (1,2) number is identical.
2. all-fiber completely solidified biological micro-array chip scanner according to claim 1 is characterized in that, described several laser instruments (1,2), its wavelength excitation wavelength with its corresponding biochip coloring agent respectively are identical.
3. all-fiber completely solidified biological micro-array chip scanner according to claim 1 is characterized in that, described wavelength division multiplexer (3), and its operation wavelength is identical with the wavelength of its corresponding laser instrument (1,2).
4. all-fiber completely solidified biological micro-array chip scanner according to claim 1 is characterized in that, described optical collimator (5) is optical fiber GRIN Lens or Liar.
5. all-fiber completely solidified biological micro-array chip scanner according to claim 1 is characterized in that, described wave filter (7,8), and its operation wavelength is identical with its corresponding laser instrument (1,2) institute's excited fluorescent wavelength respectively.
6. all-fiber completely solidified biological micro-array chip scanner according to claim 1 is characterized in that, described photo-detector (9,10) is photomultiplier, avalanche diode, photodiode or semiconductor optical device.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200510028878 CN1731152A (en) | 2005-08-18 | 2005-08-18 | All-fiber completely solidified biological micro-array chip scanner |
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| CN 200510028878 CN1731152A (en) | 2005-08-18 | 2005-08-18 | All-fiber completely solidified biological micro-array chip scanner |
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| CN1731152A true CN1731152A (en) | 2006-02-08 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101589303B (en) * | 2007-01-17 | 2012-01-11 | 霍尼韦尔国际公司 | Microarray reader based on evanescent wave detection and method of reading a microarray |
| CN103775951A (en) * | 2012-10-19 | 2014-05-07 | 上海铭源数康生物芯片有限公司 | Light-emitting diode (LED) array based standard plane light source |
-
2005
- 2005-08-18 CN CN 200510028878 patent/CN1731152A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101589303B (en) * | 2007-01-17 | 2012-01-11 | 霍尼韦尔国际公司 | Microarray reader based on evanescent wave detection and method of reading a microarray |
| CN103775951A (en) * | 2012-10-19 | 2014-05-07 | 上海铭源数康生物芯片有限公司 | Light-emitting diode (LED) array based standard plane light source |
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