CN1703148A

CN1703148A - System and preparation method for synergistic disinfection and purification of organic compounds and metal ions

Info

Publication number: CN1703148A
Application number: CNA2003801011713A
Authority: CN
Inventors: 布雷德利·J·埃尔德雷德
Original assignee: Individual
Current assignee: Individual
Priority date: 2002-10-08
Filing date: 2003-10-07
Publication date: 2005-11-30
Anticipated expiration: 2023-10-07
Also published as: ES2357760T3; CN1703148B

Abstract

The present invention discloses a new and useful disinfection and purification system consisting of organic compounds and selected metal ions having a synergistic effect, as well as a method of preparation and use. One of the advantages of this system is that surfaces, fluids or media can be quickly and easily treated to disinfect bacteria, molds, viruses, algae and protozoa using a non-toxic, non-chlorinated mixture that effectively kills all such organisms within minutes or hours. The disinfection and purification system remains effective for long periods of time, maintaining its disinfection capabilities, thereby making it highly effective for storing or transporting media such as potable drinking water. The disinfection and purification system may be used in conjunction with a carbon block or membrane filter to disinfect filtered liquids, such as water, for potable purposes. The disinfection and purification system may be infused into a semi-permeable surface or applied to a non-porous surface such that the disinfectant compounds of the system are delivered to the surface of the object for disinfection by leaching, capillary action, pure contact, water activation and/or permeation/diffusion.

Description

The system of organic compound and metal ion synergistic disinfection and purification and preparation method

The invention technical field

The present invention relates to be mainly used in the bactericidal composition of disinfectant, the organic compound that the said composition utilization is selected and the concurrent disinfection performance of selected metal ion.The sterilization invention can be used for including but not limited in the various application: public water system (PWS); Bottled water/stock water; Use a little/inlet point (POU/POE) system at home, be used for the urgent water sterilization of disaster form, military, travelling usefulness, camping usefulness, mountain-climbing is used, commercial distribution and/or batch processing device, hydrocooler/distributor, food surface sterilization, fruits and vegetables sterilization rinsing, meat, fish and poultry sterilization, fruit juice and beverage sterilization (comprising apple juice), the ice preparation of commercialization/house, medical and tooth device, hot spring, drinking water and the recirculation water used in as the pond, industrial treatment and waste water system, steamer, aircraft, recreational vehicle and space.The sterilization invention also is widely used in the drinking water disinfection of the third world and developing country.When using each constituent concentration of drinking water of government's management and control or recommendation, the present invention can several minutes or hour in water sterilization efficiently is provided, remaining decontaminant concentration is an effective dose.When do not need several minutes or hour disinfect the time also can use the present invention during in the concentration that reduces basically, and still can make water sterilization effectively, as stocking under the regimen condition of planning directly to use.When the restriction of inapplicable this drinking water concentration or when recommending, when being higher than the drinking water of management and control or recommendation, concentration also can use the present invention, as the food rinsing, industrial treatment and recreational water.

The present invention can be used as independent disinfectant processing or can use with other purification techniques, filters or other microsieve filtration technology as carbon brick.This extra purification techniques can be used to remove degranulation, organic/inorganic impurity and taste/stink, and provides and provide effective size exclusion to remove method to big microorganism such as Cryptosporidium and Giardia.The present invention can use with other disinfection technologies, as ultraviolet light, oxidative chemicals and thermal treatment, so that organic matter such as Cryptosporidium and Giardia inactivation or kill them.

The present invention utilizes selected metal ion and natural plant extracts and/or alcohol as disinfectant.

Background technology

Federal and management and control mechanism of government, world's publilc health tissue and the non-expert public think that the microbial safety drinking water is basic needs.Can not overemphasize more effective, available, extensive use and easy-to-use water treatment disinfection technology advantage to flourishing (industrialization) and developing country etc.

World health organization (WHO) estimate that world population about 20% or 1,700,000,000 people lack improvement with the safe water supply, therefore annual 3～4 million peoples especially children because of dangerous drinking water death.In the U.S., U.S. CDC (CDC) estimates, comprises report and the not morbidity of report, and annual have 940,000 cases and possibility 900 people's death because of infecting through water microorganism.Some people comprises that ill other people of baby, the elderly, organ transplant patient, cancer therapy patients, AIDS patient and immune system are easier to suffer from through water borne disease.

U.S. environmental protection mechanism (EPA) regulation and the management and control U.S. surpass the drinking water quality of 170,000 public water systems (PWS).Wherein, surpass 160,000 PWS and be divided into " little " or " minimum " class, and serve 25 and 3,300 people.The present invention can be used as the disinfectant of PWS.EPA has recognized that many shortcomings of chlorine (among the PWS disinfectant of normal use), and this is very important.EPA has produced interest to the new water technology selected, and investment is to its research.Making the significant disadvantages of disinfectant with chlorine is to form disinfection byproduct (DBP) (DBP), and some of them are poisonous and may be carcinogen.This has limited the use of chlorine and/or has required extra processing to form to reduce DBP.In addition, the physics of water to be clean and/or chemical property have limited the effect of chlorine.The restrictive factor of chlorine purifying functions comprises the existence of water temperature, pH, organic carbon, the organic and inorganic matter and the turbidity of dissolving.Use chlorine is made disinfectant and is also had taste and bad-smell problem and and the relevant problem of processing chlorine.

The plurality of optional of its current approval extensive use of drinking water disinfection of having selected technical limitations in the disinfectant selected of EPA and oxidant Guide Book.In addition, many selectable technology still form DBP and/or inorganic byproduct.Except that chlorine dioxide and monochloramine, these alternative disinfectants all can not provide remaining sterilization in distribution system or container, therefore need to replenish the chlorine of some form of use.

For becoming real effective and powerful drinking water disinfect and multiple application, need be to bacterium and virus effective disinfectant/disinfectant all.

To other selections of chlorine, all can not obtain surpassing the inactivation virus of 2-log usually as ion, permanganate, chloramines and ozone.In some cases, these alternative disinfectants can not provide measurable inactivation virus, especially in the water of high muddy degree, high content of organics.CDC recommends to use iodine, uses but only be used for short-term emergency, and has significant adverse taste and aesthetic drawbacks.In a word, there is not the alternative disinfectants of approval can obtain surpassing inactivation bacterium and the virus of 3-log always, and also can avoid DBP to form and effective disinfectant remnants are provided, to store at long-time water and/or microorganism inoculates back (taking place usually) microbial safety water is provided when water is stocked in actual use.

Except PWS, new effective water sterilization safety method has multiple extra application.These are included in the office of the microbial quality that needs the pass edema with the heart involved and the water dispenser in the public building, outdoor activities are as the drinking water treatment of travelling, camping usefulness, recreational vehicle is used, encamp and camp season, use point (POU) device as demineralizer and carbon filter, the driving water treating apparatus, the fruits and vegetables rinsing, meat, fish and poultry rinsing, the storage of commercial fishing work and transportation ice, military as the far ultraviolet use, military big water gaging is stored or is handled, and steamer, aircraft application, NASA (NASA) uses etc.

Handle undrinkable water and make its microbial safety, generally include treatment technology such as sub-micron filtration, ultraviolet light, ozone, chlorine, iodine and/or other sterilization chemical to be fit to usefulness at home.Some treating apparatus utilize resin or the medium through biocidal compound such as iodine dipping, make this biocide of certain level discharge in the water inlet.Although size exclusion and ultraviolet light based system can be removed certain micro-organisms basically immediately or be made its inactivation, they can not provide the disinfectant of any remnants to prevent the organism regrowth.Particle, high content of organics, turbidity and number of chemical composition are handled the ultraviolet light base tangible adverse effect, therefore is not suitable for polytype water.They also need certain class power supply, and no matter be (interchange) AC or (direct current) DC in essence.The size exclusion based method is effectively for removing virus removal and some smaller bacteria usually, and limits output influenced because of filter blocks.

The invention provides the new disinfection technology that utilizes metal ion and natural derivative mixture.It can be widely used in the untreated natural waters that shows multiple physico and/or the municipal water of processing.It also is widely used for the undrinkable water application as previously mentioned, comprises recreational water processing (heating bath box, swimming pool, treatment pond), the dentistry production line apparatus, and food preparation, water is usefulness again, industrial cooling circuit, cooling tower etc.It can be used as disinfects separately or can use with other water technologies.

Although separately, mix with other ions or mix the use metal ion with other materials such as free chlorine and hydrogen peroxide and in some water type, obtaining certain success aspect the inactivation of bacteria, do not have scientific literature report certain mixing can be after comprising a few hours or a couple of days longer time of contact to multiple test water continuously effective aspect virally inactivated.When the effective target of in attempting to prepare, determining and the microbial safety drinking water of standard based on the guidance standard of the surface water processing rule (SWTR) of EPA and/or its test microbes water purifier and agreement, and especially true when using short time of contact.Similarly, although non-expert has opposite requirement, after the test of costliness, when independent use Citrus extract during, even when pure water has long-term time of contact under as the municipal water handled or untreated natural waters (as the water of obtaining) test condition from river, small stream, river, pond, lake, spring or well, can not reduce virus effectively as the plant extracts that in most preferred embodiment of the present invention, uses.The mixing of the really independent ion of laboratory tests or independent plant extracts or ion and glycerine all can not reduce virus effectively.Under identical and harsher test condition, it is virally inactivated that identical test confirms that always the present invention can make.The mixing of using independent ion, independent plant extracts or ion and alcohol (glycerine) is to testing the result below obtaining through the various water chamber of experimentizing of bacterium and/or virus inoculation:

After municipal water matrix 60 minute contact time of the mixed copper of copper/silver ion-independent silver ion of use or specific ratio and silver ion (10: 1～30: 1) and inoculation Klebsiella is reduced 5log and 6log respectively separately.Copper ions alone is not effectively, with time of contact of the municipal water 60 of inoculation and 240 minutes after reduce respectively less than 3log and 5log.After 90 minute contact time, the maximum reduction of Klebsiella to be compared less than 5log, copper/silver ion treatment is lower to the Pseudomonas aeruginosa effect in the municipal water of inoculation.

To using independent silver or copper ion with the untreated natural waters of Klebsiella inoculation as the water of obtaining from river, small stream, river, pond, lake, spring or well water, its log reduces less than 3-log after 120 minute contact time.After 120 minute contact time, use its log of combined silver and copper ion to reduce less than 4-log.

In test specification, being proved independent copper/silver ion that MS2 virus is had an edge effect does not have log to reduce in minimum time of contact in for example less than 24 hours.Do not observe MS2 virus after 6 hours times of contact and reduce, in the time of 24 hours, observe less than 3-log virus and reduce.Untreated natural waters to inoculation obtains similar as the water of obtaining from river, small stream, river, pond, lake, spring or well or more obviously reduces the inactivation result, uses copper or copper/silver ion to obtain inactivation less than 1log after 4 hour time of contact.Even after using the municipal water of independent silver ion and inoculation or untreated natural waters to reach 24 hours time of contact, do not reduce MS2 effectively as the water of from river, small stream, river, pond, lake, spring or well, obtaining yet.Therefore, use independent copper and/or the silver ion virus of can not sterilizing satisfactorily.

In a word, no matter be used singly or in combination independent ion pair municipal water or untreated natural waters all can not obtain acceptable sterilization as bacterium in the water of obtaining and virus from river, small stream, river, pond, lake, spring or well.

Plant extracts, promptly independent Citiricidal ^TM-use no matter be the independent Citricidal that is dissolved in water or the glyceryl ^TMWater with the Klebsiella inoculation is all obtained inconsistent performance.The log of municipal water to inoculation after 60 minutes is reduced to 4log～6log.Even after 120 minutes or the longer contact, the untreated natural waters of inoculation observed to log as the water of obtaining from river, small stream, river, pond, lake, spring or well reduce less than 3log.Citricidal ^TMUsually invalid fully to MS2 virus, though with do not observe reduction after the municipal water of inoculation or the untreated natural waters of inoculation contacted as the water of obtaining in 24 hours from river, small stream, river, pond, lake, spring or well yet.

In a word, plant extracts (is Citricidal ^TMAll can not obtain acceptable sterilization to municipal water or untreated natural waters as bacterium in the water of from river, small stream, river, pond, lake, spring or well, obtaining and virus separately).

Metal ion that mixes and glycerine (alcohol)-mixing uses copper and silver ion and glycerine that the water with the Klebsiella inoculation is all obtained inconsistent performance.The log of municipal water to inoculation after 60 minutes is reduced to 5log～6log.Nonvaccinated untreated natural waters is reduced less than 1.5log as the heterotroph log in the water of obtaining from river, small stream, river, pond, lake, spring or well.Municipal water or untreated natural waters to inoculation use the ion and the glycerine that mix not to observe the reduction of MS2 virus as the water of obtaining from river, small stream, river, pond, lake, spring or well.

In a word, the ion of mixing and glycerine all can not obtain acceptable sterilization to municipal water or untreated natural waters as bacterium in the water of obtaining and virus from river, small stream, river, pond, lake, spring or well.

As previously mentioned, the invention provides unique metal ion and natural plant extracts and alcohol that mixes.Therefore, it is a new method, and does not appear in the newspapers in document or existing patent.Before this, many researchers attempt to find suitable selectable chlorination, but each all has shortcoming.The researcher attempts to utilize the anti-microbial property of copper and silver ion, by with them and various other its effects of composition hybrid optimization, especially to virus.Yet former effort can not be developed in than short contacting time bacterium and the viral any copper/silver-colored mixing disinfectant that obtains acceptable inactivation.Some correlative studys are summarized as follows.

Domek etc., be disclosed in Domek, M., M.LeChevallier, S.Cameron and G.McFeters, 1984, Evidence for the Role of Copper in the Injury Processof Coliform Bacteria in Drinking Water, among the Appui Environ.Microbiol.48:289-293, the E.coli in the existence of the low-level copper infringement drinking water sample, and this effect is that dosage is relevant.Test shows subsequently reduces because of the picked-up of cell oxygen and the glucose utilization of copper loss evil, and metabolic end product changes.

Kutz etc., as be disclosed in Kutz, S., L.Landeen, M.Yahya and C.Gerba.1988, Microbiological Evaluation of Copper:Silver Desinfection Units, Proceedings of the Fourth Conference on Progress in ClinicalDisinfection, S.U.N.Y., Binghamton, NY, April 11-13,1988, detect independent copper (Cu) and silver (Ag) ion that electrolysis generates, free chlorine (FC) reaches seven types of bacteriums of Cu/Ag ions plus low levels FC antagonism separately.Their result shows, compares with independent processing, and all bacteria tested are faster with the mixed processing inactivation.

Landeen etc., as be disclosed in Landeen, L., M.Yahya and C.Gerba 1989, effect of Copper/Silver Ions ﹠amp; The horizontal s of of Reduced Free Chlorine inInactivation of Legionella prreumophila, Appl.Environ.Microbiol.55:3045-3050, also use Legionella as the target organism at copper test and silver ion in the presence of the FC or not, report that the sterilization statistical significance with mixed processing improves.

Yahya etc., as be disclosed in Yahya, M., L. Landeen, M.Mesina, S.Kutz, R.Schultze and C.Gerba, Desinfection of Bacteria in water Systems byUsing Electrolytically Generated Copper:Silver ﹠amp; The horizontal s of of Reduced FreeChlorine, Can J.Microbiol.36:109-116, use Staphylococcus sp. to carry out similar test, before this studies show that comparing processing with colon bacillus more has patience, and to FC is added in the processing advantage and separately the restriction of copper/silver ion obtain similar conclusion.

Margolin etc., as be disclosed in Margolin, A.B.Control of Microorganisms inSource Water and Drinking Water.Pp.274-284, In:Manual ofEnvironmental Microbiology, Hurst, C.J., Ed., ASM Press, WashingtonD.C., 2002, be evaluated under the situation of FC adding and not adding, dipping copper makes MS2 and polyovirus inactivation.They report that sterilization shows more great patience (being respectively 1.3log and 4.0log inactivation in 12 hours) than MS2 to polyovirus to copper, and add the inactivation that FC obviously strengthens these two kinds of Virus Types.

Abad etc., as be disclosed in Abad, F., R.Pinto, J.Diez and A.Bosch, 1994, the horizontal s of of Disinfection of Human Enteric Viruses in Water by Copped Silver inCombination with Low Chlorine, Appl.Environ.Microbiol.60 (7): 2377-2383, copper test and silver ion mix enteroviral effect with low-level FC.They report and add silver by copper to add 0.5mg/L FC more ineffective to polyovirus than independent 1mg/L FC.The author also observes under test condition, and the contact of 120 minutes disinfectant of adenovirus require reduces to obtain 3log, and hepatitis a virus (HAV) and human rotavirus have more patience.The author thinks, (when using) but copper and silver in the water makes the high-level FC of viral pathogens sterilization that candidate reliably can not be provided for being used to.Yet they have also reported copper and the stability of silver-colored level in test cabinet, and initial input is respectively after 75% and 44%, 60 day and can detects.

Lin etc., as be disclosed in Lin, Y., R.Vidic, J.Stout and V.Lu, 1996, Individualand Combined effect s of Copper and Silver Ion on Inactivation ofLegionella Pneumophila, Wat.Res.30 (8): 1905-1913 detects independent copper and silver ion and the mixing effect to L.pneumophila serum group 1.These authors notice that copper is more effective than independent silver, are 2.5 hours but need time of contact, so that complete (6log) inactivation of Legionella (silver needs 24 hours).

Obviously, Lin etc. also notice and depend on used concentration, and copper and silver ion can obtain addition or synergy, and think that their immixture is greater than from independent copper or silver-colored observed effect.

Rohr etc., as be disclosed in Rohr, U., S.Weber, F.Selenka and M.Wilhelm, 2000, Impact of Silver and Copper on the Survival of ameba and cilium Protozoa in Vitro, Int.J.Hyg.Environ.Health 203:87-89 detects at external copper and silver ion ameba and the protozoic effect of cilium.The author reports in German drinking water management and control restriction (Ag and Cu are respectively 10 and 100mg/L), and mixed processing does not make tests the protozoa inactivation.

Batterman etc., as be disclosed in Batterman, S., K.Mancy, S.Wang, L.Zhang, J.Warila, O., Lev, H.Shuval and B.Fattal, 2001, Evaluation ofthe effect of a New Secondary Disinfectant Formulation Using Hydrogen peroxide and Silver and the Formulation of Disinfection By-productsResulting From Interactions With Convention Disinfectants, EPA STARGrant No.R825362, in EPA STAR work, assessment hydrogen peroxide (H ₂O ₂) add the effect of copper and silver ion mixed processing.For bacterium, they report that hydrogen peroxide is lower than silver ion efficient, and silver ion compares H ₂O ₂Low with the silver ion mixing efficiency, and H ₂O ₂With silver ion mixing ratio H ₂O ₂It is low to add copper ion efficient.Yet it is unacceptable virally inactivated that the author thinks that mixing disinfectant can obtain.Make MS2 colibacteriophage inactivation 4log need 6 hour time of contact, poorer to the effect of polyovirus, only obtain the inactivation of 0.15log after 12 hour time of contact.

From the top research that this states as can be seen, although separately or other materials mix and use copper and silver ion that inactivation of bacteria is had certain success, in short contacting time to the virus in the multiple test water not combination be proved effectively.

Similarly, the prior art patent disclosure example of multiple antibacterial agent, the metal ion that it comprises separately or mixes with other materials.Yet the prior art patent is instructed the decontamination system that is used for undrinkable water usually, as swimming pool and hot spring.In several examples, the prior art patent comprises the treatment system that is used for drinking water.These prior art patents are not all instructed and are used the EPA of plant extracts, pure and mild metal ion to drink concentration, so that contain the water sterilization of bacterium, algae, protozoa, virus and mould, thereby effectively kill speed several minutes or hour in impure before this water source can be drunk, and provide disinfectant remnants, with the several months or year in remain valid ground killing bacteria and virus or make its inactivation, and do not form disinfection byproduct (DBP).

Disclosure of the Invention

In view of the not expection or instruct organic compound and selected metal ion that synergy is arranged of the inherent limitations of known type sterilization and purification systems and prior art, the invention provides a kind of by organic compound and sterilization and the purification systems formed by synergistic action with selected metal ions, and preparation method and purposes, it is designed to allow fast and effectively disinfecting surface of user, microorganism in medium or fluid such as the water is (as bacterium, virus, protozoa, algae and mould), this similar decontamination system with patent applied for or commercialization is obviously different, and is a kind of improvement.Thereby (the following detailed description) of catalogue of the present invention provides a kind of new and practical and by organic compound and sterilization and the purification systems is made up of synergistic action with selected metal ions, reaches preparation method and purposes.The present invention has many extra new features, relates to the problem that solution is not solved in the prior art, and is as follows:

-mixture of active principles, it is specified to user's designated surface with the user and produces the collaborative antibacterial action that strengthens in medium or fluid such as the water; And

-independent treatment technology is used to make water sterilization, and the feasible water of seeing from bacterium, virucide, mould, marine alga and protozoa angle can be drunk safely.

In the most preferred embodiment, the composition of treatment mixture is made up of following material usually:

1. mixture of active principles, it comprises:

A) one or more water soluble metallic compounds (including but not limited to solution metal sulphate, slaine or metal citrate or some other metal ion sources) are as one or more metal ion solution sources that are selected from the metal among the 1b of periodic table of elements family, 2b, 3b, 4b, 5b, 6b, 7b, 8,3a, 4a and the 5a;

B) one or more plant extractss; And

C) one or more alcohol.

The composition of treatment mixture also can be made up of following material:

2. mixture of active principles, it comprises:

A) one or more water soluble metallic compounds (including but not limited to solution metal sulphate, slaine or metal citrate or some other metal ion sources) are as one or more metal ion solution sources that are selected from the metal among the 1b of periodic table of elements family, 2b, 3b, 4b, 5b, 6b, 7b, 8,3a, 4a and the 5a; And

B) one or more plant extractss.

In the another kind of composition of treatment mixture, it also can be made up of following material:

3. mixture of active principles, it comprises:

B) one or more alcohol.

Although common main purpose of the present invention is to avoid the use of disinfectant, as chlorine, radiation, ozone and temperature etc., but the following composition of treatment mixture comprises these disinfectants, but obviously reduction of concentration and/or exposure is used side effect thereby reduce it:

4. mixture of active principles, it comprises:

B) one or more plant extractss; And/or

C) one or more alcohol; And/or

D) one or more sterilization compounds are as ozone or be selected from halogen or derivatives thereof such as halogenated hydrocarbons, acid amides, amine or the halo dioxide of the 7a of periodic table of elements family; And/or

E) in case mixture is applied to disinfecting substance or mixes with it, make mixture, comprise ultraviolet and infrared etc., sound wave explosion and cold or hot limiting temperature as radiation through antibiotic physics challenge or processing.

For understanding following detailed description better, for understanding the contribution of the present invention better, very broadly understand key character of the present invention simultaneously to prior art.Hereinafter additional features of the present invention will be described, in case submit application comprehensively to, this will become the theme of claim.

In this connection, before describing at least a embodiment of the present invention in detail, should be appreciated that the present invention in application, method and apparatus are not limited to the following describes CONSTRUCTED SPECIFICATION described in the book and element arrangements.The present invention, method and apparatus can become other embodiments, and can implement in every way and carry out.In addition, should be appreciated that word used herein and term be used for the explanation, do not limit the present invention in any way or claims in protection domain.

Therefore, will be understood by those skilled in the art that open based on design can be at an easy rate as other structural designs of implementing the several purposes of the present invention, the basis of method and system.Therefore importantly claim is believed to comprise this equivalent construction and method, as long as they do not break away from the spirit and scope of the present invention.

In addition, the purpose of above-mentioned summary is that this area scientist, engineer and the practitioner who makes United States Patent (USP) trademark office and the general public, particularly is unfamiliar with patent and law term or word can understand the essence and the characteristic of present techniques content apace by skimming.Summary also is not used in restriction the application's invention, and the application is defined by the claims, the protection domain that does not also limit the present invention in any way.

Therefore, an object of the present invention is to provide a kind of new and practical and by organic compound and sterilization and the purification systems formed by synergistic action with selected metal ions, and preparation method and purposes, it has the multiple advantage of above-mentioned sterilization and purification systems and has many new features, thereby obtain by organic compound and sterilization and the purification systems formed by synergistic action with selected metal ions, and preparation method and purposes, in the sterilization of any prior art of independent or any combination and purification systems and manufacture method, this all not have to expect, obviously, suggestion or even hint.

Another object of the present invention provides a kind of by organic compound and sterilization and the purification systems be made up of synergistic action with selected metal ions, and it can be easy to and be produced efficiently, instructs, sells and used by the terminal use.

Another object of the present invention provides a kind of by organic compound and sterilization and the purification systems be made up of synergistic action with selected metal ions, and it is durable and reliable structure.

Another object of the present invention provides a kind of by organic compound and sterilization and the purification systems be made up of synergistic action with selected metal ions, the material of its preparation and cost are all lower, can lower price be sold to the user thus, thereby make this economical and practical for the public who buys with sterilization of forming by synergistic action with selected metal ions and purification systems by organic compound.

Another object of the present invention provides a kind of by organic compound and sterilization and the purification systems be made up of synergistic action with selected metal ions, the disinfectant remnants that it all can be remained valid in several months or year.Up to the present, the present invention keeps its sterilization and catharsis in 21 weeks.Collect the municipal water sample (dechlorination) of preferred embodiment of the invention protection concentration, and under environmental condition, preserve.After initial treatment, regularly remove two parts of samples of the water of processing, and to be seeded to concentration be 10 ⁶～10 ⁷The Kelbsiella/ml sample.In the collection period in 21 weeks, when 30,60 and 90 minute contact time, the log of Klebsiella reduction remains unchanged complete deactivation when 60 and/or 90 minute contact time.This test is still being carried out, and can expect the present invention to the water sample of stocking with the present invention's processing, the ability of the ground killing bacteria of should sustainablely remaining valid.This is the feature of the only thing of the present invention keeping the ability of disinfectant remnants effectively extremely for a long time.The result of remaining efficacy study shows that in Fig. 5 its data according to Fig. 6 A, Fig. 6 B and Fig. 6 C are prepared from.

Another object of the present invention provides a kind of sterilization mechanism, and it allows to make in the water sample of the processing of repeated contamination/inoculation and storage microorganism deactivated.In fact, this is meant that the present invention is not consumed in the first time and disinfection reactions subsequently.Ability of the present invention is by a series of experiment confirms.Collect the municipal water sample (dechlorination) of preferred embodiment of the invention protection concentration, and under environmental condition, preserve.Store after one month, remove the sample of the water of processing, and with 10 ⁶～10 ⁷The inoculation of Kelbsiella/ml sample.Then, in cycle time at interval the time, Klebsiella inoculates same sample with same concentrations, and further adding disinfectant not.This thing happens in 21 weeks, and still carrying out.After five inoculations, when 30,60 and 90 minute contact time, the inactivation effect of Klebsiella remains unchanged, and observes to killing (6～7log) fully in the time of 90 minutes.Since the 6th inoculation, when 30 minute contact time, observe kill efficacy and reduce.After 3 extra inoculations, observe kill efficacy and reduce when 90 minute contact time, although after 120 minute contact time, (7log) kills the Klebsiella of inoculation fully.Ongoing Therapy lasted is followed the tracks of, and comprises and surpass 90 minutes longer time of contact, to be evaluated at the ability that the invention provides effective sterilization after the extra inoculation.The result who repeats subject study shows that in Fig. 7 its data according to Fig. 8 A, Fig. 8 B and Fig. 8 C are prepared from.

Another object of the present invention provides a kind of disinfectant solution or material, and its final mixed form shows the longer shelf-life and do not need special storage requirement.Laboratory tests confirm this really situation of the present invention.Make the working solution of four kinds of constituent concentrations of most preferred embodiment of the present invention, then at South Hero, in the not controlling temperature environment of VT, indoor storage is 13 months in airtight container.This preparation in storage period through the temperature of-20～100+, repeatedly freezing and thaw.It is stored in the translucent container, and through nature daytime and evening, stores the disinfectant that compares " storage " disinfectant and new system after 13 months.To with 10 ⁶～10 ⁷The performance of the municipal water of Kelbsiella/ml sample inoculation is not observed the significant difference between new system and " storage " disinfectant, kills the organism of inoculation in 60～90 minute contact time fully.

Another object of the present invention provides a kind of by organic compound and sterilization and the purification systems be made up of synergistic action with selected metal ions, and some advantage that it has the prior art system has overcome the shortcoming of prior art simultaneously.

Of the present invention these, other purposes and various feature of the present invention will list in claims.For understanding the present invention, its service advantages and specific application target better, should the preferred embodiments of the invention have wherein been illustrated with reference to appended explanation.

Implement best mode of the present invention

The most preferred embodiment-in the most preferred embodiment, the present invention uses two kinds of selected metal ion copper and silver-colored Drinking Water, the solution that it is selected from copper sulphate or copper citrate and silver sulfate or silver citrate and places pending water.The metal ion of dissolving mixes with plant extracts that is selected from the shaddock seed and pure glycerine.In the most preferred embodiment, activity component concentration should be in final water:

A) be that 750ppb is no more than government's management and control admissible concentration (as EPA applicatory or WHO standard) that the user selectes at most in copper in drinking water;

B) silver is no more than government's management and control admissible concentration (as EPA applicatory or WHO standard) that the user selectes at most for 37.5ppb in drinking water;

C) where applicable shaddock seed extract (is Citricidal in drinking water ^TM) reach 80-110ppm (i.e. 10 or 100mg Nutribiotic ^TMBrand shaddock seed extract) is no more than government's management and control admissible concentration (as EPA applicatory or WHO standard) that the user selectes at most; And

D) where applicable, glycerine reaches 160-220ppm and is no more than government's management and control admissible concentration (as EPA applicatory or WHO standard) that the user selectes at most in drinking water.

In drinking water treatment, stock solution can be produced, and makes the copper/silver-colored storage capacity that is added in 1 premium on currency be respectively 1.5ml and 0.75ml.By regulating stock solution intensity, so if this will be quite easily change 5 gallons of volumes-handle (20L+/-) water, do not need to add 30ml copper and 15ml silver so, consider to use obvious a spot of volume from packaged convenience and other relevant issues angles on the contrary, but still have disinfection efficacy.Be called as Citricidal ^TMCommercial shaddock seed extract (GSE) usually its commercial packing be 33%Citricidal ^TMLiquid form with 67% glycerine.This has determined above-mentioned Citricidal ^TMWith the glycerine ratio.Yet, the Citricidal of use dry powder form ^TMCan make these two kinds of component ratios different with glycerine.In addition, use commerce Citricidal easily ^TMNot necessarily.Other plant extract independent or that mix with equivalent all has disinfecting power.

Show effective sterilization with 1: 1～50: 1 bronze medal/silver ion ratios.Copper and silver ratio are to have best disinfecting power at 20: 1 o'clock.For the present invention who uses long time of contact used, as for the water of stocking of not planning to use immediately, ion when any or all each constituent concentration that comprises that the present invention forms all was proved and can obviously reduces.The ability that reduces concentration when length during time of contact is common and consistent by CT value (concentration * time) notion of the theoretical establishment of classics sterilization.Therefore, if the concentration of these compositions does not satisfy government's management and control level obviously, can use higher concentration so, therefore improved its antibiotic validity, otherwise or, if be suitable for longer time of contact, concentration so will be lower so, and the present invention is still effective for drinking water.Because its high viscosity is used " dripping " to measure GSE and (is used commercial Citricidal ^TM/ glycerol mixture).Use liquid-drop machine to measure 1 milliliter of Citricidal ^TM40 of/glycerine, and be added in the commercial GSE bottle.When in 1 liter of pending water, using 10 Citricidal ^TMDuring/glycerol mixture, its concentration is corresponding to 0.25ml Citricidal ^TM/ glycerine/premium on currency.About 0.125ml Citricidal ^TM/ glycerine or still less with copper and the silver ion synergy 1 liter of filtered water of just can sterilizing effectively, and every liter of turbidity need about 0.25ml Citricidal ^TM/ glycerine.

The concentration of most preferred embodiment does not change basically in time in the time of in being dissolved in water, therefore realizes effectively " immediately " sterilization and can provide effective sterilization remaining.This specific character of the present invention is specially adapted to keep stocking the drinkable property of water, and this is might be inoculated by unwanted microorganism owing to stocking water.

Use the most preferred embodiment to carry out initial validation test, and comprise the test of each independent compound, combined experiments then is to obtain having the most preferred embodiment of maximum concurrent disinfection ability.Various test water are carried out initial trial, comprise the municipal water of filtration, dechlorination, self-faced water (water of untreated natural waters) and synthetic water (EPA test water #3-is disclosed in the EPA guidance standard) as from river, small stream, river, pond, lake, spring or well, obtaining.Use the various water types of Klebsiellaterrigena, Pseudomonas aerugiwsa and MS2 bacteriophage seeded to test.Bacterium is seeded to usually and is initially 6～7log concentration (colony-forming units, CFU, every ml sample); Virus inoculation is to obtain 5log concentration (plaque forming unit (PFU), every ml sample) in each test water matrix.

With the Citricidal in copper and silver ion and the glyceryl ^TMMix to produce the obvious synergistic effect, compare with any separate constituent or partially mixed composition, disinfection efficacy is improved basically.Carry out repeated test/test confirmation in two years, use the present invention all to make bacterium and virus have higher log to reduce to the municipal water of inoculation and the untreated natural waters of inoculation as the water of obtaining from river, small stream, river, pond, lake, spring or well, the sample that makes can be drunk by microorganism according to EPA and WHO standard.

The most preferred embodiment made the Klebsiella complete deactivation (6+log) in the municipal water of inoculation in 30 minutes, (turbidity＞30NTU, total organic carbon (TOC)＞100mg/L) have 4+log to reduce as the water obtained from river, small stream, river, pond, lake, spring or well to the untreated natural waters of inoculation in 60 minutes.P.aeruginosa in the municipal water of inoculating in 90 minute contact time kills (6+log) fully.

The synergy of most preferred embodiment is more obvious to virus.MS2 complete deactivation (5+log) in the municipal water of in 15～30 minutes time of contact, inoculating.The untreated natural waters of inoculation as the water of from river, small stream, river, pond, lake, spring or well, obtaining in (turbidity＞10NTU and TOC＞200mg/L), MS2 (5+log) inactivation fully in 30～60 minutes.In the synthetic water of inoculation (turbidity＞30NTU, TDS 1500mg/L is consistent with the disclosed EPA test water of EPA guidance standard #3 with pH 9.0-), MS2 reduces 4+log in 60 minutes, complete (5+log) inactivation in the time of 120 minutes.

Test result in Fig. 5, Fig. 6 A, Fig. 6 B, Fig. 6 C, Fig. 7, Fig. 8 A, Fig. 8 B and the most preferred embodiment shown in the 8C clearly confirmed and any separate constituent or partially mixed comparing, and (is Citricidal with copper and silver ion and shaddock seed extract ^TM) and glycerine mix in a manner described and improved disinfecting power basically.

Implement cascade filter type of the present invention

Mixed copper and silver ion and Citricidal in the above-mentioned best examination of the present invention ^TMWith glycerin disinfectant can with any filter coupling, as depicted in figs. 1 and 2 those carbon piece or millipore filters.This combination will filter unwanted particle or biological substance, and these materials can exert an influence to the drinkable property of water or not influence, but can produce adverse influence to its aesthetic feeling and taste.

In device shown in Figure 1, water to be sterilized and that purify at first passes through standard commercial carbon piece filter, and this moment, water was by second chamber, wherein copper and silver ion and Citricidal ^TMSet in advance with the best mode mixing disinfectant concentration of glycerine, described disinfectant (this can be sheet, liquid or powder type) exists with the scheduled volume that is enough to make the user select the water sterilization of volume.When water passed through second chamber, its physical action forced copper and the silver ion and the Citricidal of mixing ^TMEnter in the solution with glycerine.Contain position that the water of the compound of dissolving selectes by the user then as the 3rd chamber or hold-up vessel, its size is enough to hold according to used disinfectant quality sterilization and the volume water that purifies.

In another embodiment shown in Figure 2, water to be sterilized and that purify at first passes through standard commercial carbon piece filter, and this moment, water was by the identical chamber as carbon piece filter, wherein copper and silver ion and Citricidal ^TMSet in advance with the best mode mixing disinfectant concentration of glycerine, described disinfectant (this can be sheet, liquid or powder type) exists with the scheduled volume that is enough to make the user select the water sterilization of volume.In case leave carbon piece filter, because water contact sterilizing mixture, its physical action forces copper and the silver ion and the Citricidal of mixing ^TMEnter in the solution with glycerine.Contain position that the water of the compound of dissolving selectes by the user then as second chamber or hold-up vessel, its size is enough to hold according to used disinfectant quality sterilization and the volume water that purifies.

Implement the surface based fashion of inculcating of the present invention

Many industry such as food service industry need the food on the treatment surface, and the common support bacteria growth in surface, thereby may and cause human diseases even death really.Of the present invention inculcating in the surface based fashion shown in Figure 4, the semipermeable materials, polymer or the similar material that are fit to are used to make required working surface.Use mixed copper and silver ion and Citricidal in the above-mentioned best mode of the present invention then ^TMHandle material (processing can be undertaken by injection, dipping, spraying, pressure treatment and/or other mechanical devices) with glycerin disinfectant, make disinfectant as far as possible fully be comprised the material absorption of product.Fashionable when in the material manufacturing, adding as composition, the mixed copper in the above-mentioned best mode of the present invention and silver ion and Citricidal ^TMCan be added in the material structure compound substance as shown in Figure 3 and polymer with glycerin disinfectant.

As working surface the time, disinfectant keeps sterilization from the teeth outwards by leaching, capillarity, pure contact, water activation and/or infiltration/diffusion.This will prevent that bacterium from growing from the teeth outwards, thereby prevent the infectious disease diffusion, and disinfectant is transferred to the material that contacts with working surface.

About the further discussion of use of the present invention and mode of operation, from above-mentioned explanation, be obvious.Therefore, use and mode of operation further are not discussed.

About above-mentioned explanation, should be realized that, the variant that the optimum size relation of each several part of the present invention and structure comprise size, quality of materials, shape, form, function and mode of operation, assembling and use, and be clear and obvious for those skilled in the art, the present invention also comprises all equivalence relations described in the specification.

Therefore, only exemplarily illustrated principle of the present invention above.In addition because those skilled in the art can make numerous modifications and variations, therefore be not intended to limit the invention to shown in and described precision architecture and operation, so all modification and equivalents of being fit to all fall within the scope of protection of the present invention.For example, those skilled in the art obviously can reduce the concentration and the ratio of active component disclosed by the invention, and improve time of contact to obtain required sterilization result, on the contrary the concentration by improving active component and than and reduce and obtain required sterilization result required time of contact.Novelty of the present invention obviously is the concurrent disinfection effect of described material or active component group, and does not consider its relative concentration, some that should be realized that these materials than with concentration will be than described in the most preferred embodiment better.

Claims

1. sterilize and cleansing composition for one kind, it comprises:

(a) at least a metal that is selected among the 1b of periodic table of elements family, 2b, 3b, 4b, 5b, 6b, 7b, 8,3a, 4a and the 5a;

(b) at least a plant extracts; And

(c) at least a alcohol.

2. sterilize and cleansing composition for one kind, it comprises:

(a) at least a metal that is selected among the 1b of periodic table of elements family, 2b, 3b, 4b, 5b, 6b, 7b, 8,3a, 4a and the 5a; And

(b) at least a plant extracts.

3. sterilize and cleansing composition for one kind, it comprises:

(b) at least a alcohol.

4. sterilization as claimed in claim 1 and cleansing composition, wherein at least a selected metal is a copper.

5. sterilization as claimed in claim 1 and cleansing composition, wherein at least a selected metal are silver.

6. sterilization as claimed in claim 2 and cleansing composition, wherein at least a selected metal is a copper.

7. sterilization as claimed in claim 2 and cleansing composition, wherein at least a selected metal are silver.

8. sterilization as claimed in claim 3 and cleansing composition, wherein at least a selected metal is a copper.

9. sterilization as claimed in claim 3 and cleansing composition, wherein at least a selected metal are silver.

10. sterilization as claimed in claim 1 and cleansing composition, wherein selected metal is copper and silver.

11. sterilization as claimed in claim 2 and cleansing composition, wherein selected metal is copper and silver.

12. sterilization as claimed in claim 3 and cleansing composition, wherein selected metal is copper and silver.

13. sterilization as claimed in claim 1 and cleansing composition, wherein said plant extracts is selected from angiosperm.

14. sterilization as claimed in claim 1 and cleansing composition, wherein said alcohol is glycerine.

15. sterilization as claimed in claim 2 and cleansing composition, wherein said plant extracts is selected from angiosperm.

16. sterilization as claimed in claim 3 and cleansing composition, wherein said alcohol is glycerine.

17. sterilization as claimed in claim 1 and cleansing composition, wherein said plant extracts are the extracts that comes from shaddock.

18. sterilization as claimed in claim 2 and cleansing composition, wherein said plant extracts are the extracts that comes from shaddock.

19. a method for preparing sterilization as claimed in claim 1 and cleansing composition, it comprises the steps:

Select the metal among at least a periodic table of elements 1b of family, 2b, 3b, 4b, 5b, 6b, 7b, 8,3a, 4a and the 5a;

Select described plant extracts;

Select described alcohol; And

Mix described selected metal and described selected plant extracts and described selected alcohol.

20. a method for preparing sterilization as claimed in claim 2 and cleansing composition, it comprises the steps:

Select described plant extracts; And

Mix described selected metal and described selected plant extracts.

21. a method for preparing sterilization as claimed in claim 3 and cleansing composition, it comprises the steps:

Select described alcohol; And

Mix described selected metal and described selected alcohol.

22. one kind is used the sterilization of preparation method's preparation as claimed in claim 19 and the method for cleansing composition, it comprises the steps:

Inculcate mixture and enter the article that the user selectes.

23. one kind is used the sterilization of preparation method's preparation as claimed in claim 19 and the method for cleansing composition, it comprises the steps:

Mixture is coated in the surface of the selected article of user.

24. one kind is used the sterilization of preparation method's preparation as claimed in claim 19 and the method for cleansing composition, it comprises the steps:

Dissolving mixt enters the liquid that the user selectes.

25. one kind is used the sterilization of preparation method's preparation as claimed in claim 20 and the method for cleansing composition, it comprises the steps:

Inculcate mixture and enter the article that the user selectes.

26. one kind is used the sterilization of preparation method's preparation as claimed in claim 20 and the method for cleansing composition, it comprises the steps:

Mixture is coated in the surface of the selected article of user.

27. one kind is used the sterilization of preparation method's preparation as claimed in claim 20 and the method for cleansing composition, it comprises the steps:

Dissolving mixt enters the liquid that the user selectes.

28. one kind is used the sterilization of preparation method's preparation as claimed in claim 21 and the method for cleansing composition, it comprises the steps:

Inculcate mixture and enter the article that the user selectes.

29. one kind is used the sterilization of preparation method's preparation as claimed in claim 21 and the method for cleansing composition, it comprises the steps:

Mixture is coated in the surface of the selected article of user.

30. one kind is used the sterilization of preparation method's preparation as claimed in claim 21 and the method for cleansing composition, it comprises the steps:

Dissolving mixt enters the liquid that the user selectes.

31. a method for preparing sterilization as claimed in claim 1 and cleansing composition, it comprises the steps:

Select described plant extracts;

Select described alcohol;

Select at least a disinfectant compound that is selected from halogen, halogenated hydrocarbons, acid amides, amine and the halo dioxide of ozone, peroxide, the 7a of periodic table of elements family; And

Mix described selected metal and described selected plant extracts, described selected pure and mild described selected disinfectant compound.

32. a method for preparing sterilization as claimed in claim 2 and cleansing composition, it comprises the steps:

Select described plant extracts;

Mix described selected metal and described selected plant extracts and described selected disinfectant compound.

33. a method for preparing sterilization as claimed in claim 3 and cleansing composition, it comprises the steps:

Select described alcohol;

Mix described selected metal and described selected pure and mild described selected disinfectant compound.

34. one kind is used the sterilization of preparation method's preparation as claimed in claim 31 and the method for cleansing composition, it comprises the steps:

Inculcate mixture and enter the article that the user selectes.

35. one kind is used the sterilization of preparation method's preparation as claimed in claim 31 and the method for cleansing composition, it comprises the steps:

Mixture is coated in the surface of the selected article of user.

36. one kind is used the sterilization of preparation method's preparation as claimed in claim 31 and the method for cleansing composition, it comprises the steps:

Dissolving mixt enters the liquid that the user selectes.

37. one kind is used the sterilization of preparation method's preparation as claimed in claim 32 and the method for cleansing composition, it comprises the steps:

Inculcate mixture and enter the article that the user selectes.

38. one kind is used the sterilization of preparation method's preparation as claimed in claim 32 and the method for cleansing composition, it comprises the steps:

Mixture is coated in the surface of the selected article of user.

39. one kind is used the sterilization of preparation method's preparation as claimed in claim 32 and the method for cleansing composition, it comprises the steps:

Dissolving mixt enters the liquid that the user selectes.

40. one kind is used the sterilization of preparation method's preparation as claimed in claim 33 and the method for cleansing composition, it comprises the steps:

Inculcate mixture and enter the article that the user selectes.

41. one kind is used the sterilization of preparation method's preparation as claimed in claim 33 and the method for cleansing composition, it comprises the steps:

Mixture is coated in the surface of the selected article of user.

42. one kind is used the sterilization of preparation method's preparation as claimed in claim 33 and the method for cleansing composition, it comprises the steps:

Dissolving mixt enters the liquid that the user selectes.

43. one kind is used the sterilization of preparation method's preparation as claimed in claim 19 and the method for cleansing composition, it comprises the steps:

Inculcate mixture and enter the article that the user selectes; And

Make mixture inculcate article through at least a processing that is selected from radiation, light, sound wave explosion and limiting temperature.

44. one kind is used the sterilization of preparation method's preparation as claimed in claim 19 and the method for cleansing composition, it comprises the steps:

Mixture is coated in the surface of the selected article of user; And

Make the mixture coating surface through at least a processing that is selected from radiation, light, sound wave explosion and limiting temperature.

45. one kind is used the sterilization of preparation method's preparation as claimed in claim 19 and the method for cleansing composition, it comprises the steps:

Dissolving mixt enters the liquid that the user selectes; And

The liquid that makes dissolving mixt is through at least a processing that is selected from radiation, light, sound wave explosion and limiting temperature.

46. one kind is used the sterilization of preparation method's preparation as claimed in claim 20 and the method for cleansing composition, it comprises the steps:

Inculcate mixture and enter the article that the user selectes; And

47. one kind is used the sterilization of preparation method's preparation as claimed in claim 20 and the method for cleansing composition, it comprises the steps:

Mixture is coated in the surface of the selected article of user; And

48. one kind is used the sterilization of preparation method's preparation as claimed in claim 20 and the method for cleansing composition, it comprises the steps:

Dissolving mixt enters the liquid that the user selectes; And

49. one kind is used the sterilization of preparation method's preparation as claimed in claim 21 and the method for cleansing composition, it comprises the steps:

Inculcate mixture and enter the article that the user selectes; And

50. one kind is used the sterilization of preparation method's preparation as claimed in claim 21 and the method for cleansing composition, it comprises the steps:

Mixture is coated in the surface of the selected article of user; And

51. one kind is used the sterilization of preparation method's preparation as claimed in claim 21 and the method for cleansing composition, it comprises the steps:

Dissolving mixt enters the liquid that the user selectes; And

52. one kind is used the sterilization of preparation method's preparation as claimed in claim 31 and the method for cleansing composition, it comprises the steps:

Inculcate mixture and enter the article that the user selectes; And

53. one kind is used the sterilization of preparation method's preparation as claimed in claim 31 and the method for cleansing composition, it comprises the steps:

Mixture is coated in the surface of the selected article of user; And

54. one kind is used the sterilization of preparation method's preparation as claimed in claim 31 and the method for cleansing composition, it comprises the steps:

Dissolving mixt enters the liquid that the user selectes; And

55. one kind is used the sterilization of preparation method's preparation as claimed in claim 32 and the method for cleansing composition, it comprises the steps:

Inculcate mixture and enter the article that the user selectes; And

56. one kind is used the sterilization of preparation method's preparation as claimed in claim 32 and the method for cleansing composition, it comprises the steps:

Mixture is coated in the surface of the selected article of user; And

57. one kind is used the sterilization of preparation method's preparation as claimed in claim 32 and the method for cleansing composition, it comprises the steps:

Dissolving mixt enters the liquid that the user selectes; And

58. one kind is used the sterilization of preparation method's preparation as claimed in claim 33 and the method for cleansing composition, it comprises the steps:

Inculcate mixture and enter the article that the user selectes; And

59. one kind is used the sterilization of preparation method's preparation as claimed in claim 33 and the method for cleansing composition, it comprises the steps:

Mixture is coated in the surface of the selected article of user; And

60. one kind is used the sterilization of preparation method's preparation as claimed in claim 33 and the method for cleansing composition, it comprises the steps:

Dissolving mixt enters the liquid that the user selectes; And

61. one kind is used the sterilization of preparation method's preparation as claimed in claim 19 and the method for cleansing composition, it comprises the steps:

Mixture is placed filter, and the fluid that makes the user select must pass through this filter, and dissolves this mixture in the fluid that the user selectes.

62. one kind is used the sterilization of preparation method's preparation as claimed in claim 20 and the method for cleansing composition, it comprises the steps:

63. one kind is used the sterilization of preparation method's preparation as claimed in claim 21 and the method for cleansing composition, it comprises the steps:

64. one kind is used the sterilization of preparation method's preparation as claimed in claim 31 and the method for cleansing composition, it comprises the steps:

65. one kind is used the sterilization of preparation method's preparation as claimed in claim 32 and the method for cleansing composition, it comprises the steps:

66. one kind is used the sterilization of preparation method's preparation as claimed in claim 33 and the method for cleansing composition, it comprises the steps: